Publications by authors named "Shanshan Wu"

251 Publications

Colorimetric isothermal nucleic acid detection of SARS-CoV-2 with dye combination.

Heliyon 2021 Apr 21;7(4):e06886. Epub 2021 Apr 21.

Applied Biology Laboratory, College of Pharmaceutical and Biological Engineering, Shenyang University of Chemical Technology, 110142, Shenyang, China.

RT-LAMP detection of SARS-CoV-2 has been demonstrated to be a valuable diagnostic method for the diagnosis of COVID-19, which can rapidly screen carriers of the virus to effectively control the spread of the SARS-CoV-2. Here, we present a combination of dyes for isothermal detection of SARS-CoV-2 as a commercial alternative, with expanded colorimetric spectrum. We compared them with commercial reagents and proved their suitability and sensitivity through clinical RNA samples. In addition, together with commercial single dye indicators, we believe the expanded color spectrum developed here as an indicator of rapid detection will promote the diagnosis of COVID-19.
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http://dx.doi.org/10.1016/j.heliyon.2021.e06886DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059943PMC
April 2021

Long non-coding RNA LEISA promotes progression of lung adenocarcinoma via enhancing interaction between STAT3 and IL-6 promoter.

Oncogene 2021 Apr 15. Epub 2021 Apr 15.

Department of Endocrinology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China.

Long non-coding RNAs (lncRNAs) are emerging as a new class of regulators for a variety of biological processes and have been suggested to play pivotal roles in cancer development and progression. Our current study found that a lncRNA, designated enhancing IL-6/STAT3 signaling activation (LEISA, ENST00000603468), functioned as an oncogenic lncRNA in lung adenocarcinoma (LAD), a major form of non-small cell lung carcinoma, which is one of the most frequently diagnosed malignancies with high morbidity and mortality worldwide, and was involved in the regulation of STAT3 induced IL-6 transcription. Our data showed that LEISA was highly expressed in, and correlated with the clinical progression and prognosis of LAD. Ectopic expression of LEISA promoted the proliferation and suppressed apoptosis of LAD cells in vitro and in vivo. Mechanistically, we demonstrated that LEISA recruited STAT3 to bind the promoter of IL-6 and upregulated IL-6 expression. Taken together, our work identifies LEISA as a potential diagnostic biomarker and therapeutic target for LAD.
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http://dx.doi.org/10.1038/s41388-021-01769-7DOI Listing
April 2021

Association between exposure to ambient air pollutants and the outcomes of in vitro fertilization treatment: A multicenter retrospective study.

Environ Int 2021 Apr 2;153:106544. Epub 2021 Apr 2.

Center of Reproductive Medicine, Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, No. 39 Huaxiang Road, Tiexi District, Shenyang, Liaoning 110022, PR China; Key Laboratory of Reproductive Dysfunction Disease and Fertility Remodeling of Liaoning Province, PR China. Electronic address:

Background: Exposure to ambient air pollution has been reported to be inversely correlated with human reproductive health. However, the results of previous studies exploring the association between air pollution and in vitro fertilization (IVF) outcomes are conflicting, and further research is needed to clarify this association.

Objectives: This study aimed to investigate the associations between exposure to air pollutants and IVF outcomes.

Methods: We conducted a multicenter retrospective cohort study involving 20,835 patients from four cities in Northern China, contributing to 11,787 fresh embryo transfer cycles, 9050 freeze-all cycles, and 17,676 frozen-thawed embryo transfer (FET) cycles during 2014-2018. We calculated the daily average concentrations of six criteria air pollutants (PM, PM, O, NO, CO, and SO) during different exposure windows in IVF treatment timeline using data from the air monitoring station nearest to the residential site as approximate individual exposure. Generalized estimation equation models were used to assess the association between air pollution exposure and IVF outcomes.

Results: Exposure to O, NO, and CO during most exposure windows in fresh embryo transfer cycles were correlated with lower possibilities of biochemical pregnancy, clinical pregnancy, and live birth. An inverse association of exposure to O and SO with pregnancy outcomes was observed in FET cycles. In addition, we found a significant association of exposure to air pollutants with a higher risk of ectopic pregnancy and lower oocyte yield.

Conclusions: Our study provided large-scale human evidence of the association between air pollution and adverse human reproductive outcomes in the population opting for IVF. Thus, exposure to air pollutants in the population opting for IVF should be limited to improve treatment outcomes.
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http://dx.doi.org/10.1016/j.envint.2021.106544DOI Listing
April 2021

Transcriptome Regulation by Oncogenic ALK Pathway in Mammalian Cortical Development Revealed by Single-Cell RNA Sequencing.

Cereb Cortex 2021 Apr 1. Epub 2021 Apr 1.

Shanghai Key Laboratory of Psychotic Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, 200030 China.

Precise regulation of embryonic neurodevelopment is crucial for proper structural organization and functioning of the adult brain. The key molecular machinery orchestrating this process remains unclear. Anaplastic lymphoma kinase (ALK) is an oncogenic receptor-type protein tyrosine kinase that is specifically and transiently expressed in developing nervous system. However, its role in the mammalian brain development is unknown. We found that transient embryonic ALK inactivation caused long-lasting abnormalities in the adult mouse brain, including impaired neuronal connectivity and cognition, along with delayed neuronal migration and decreased neuronal proliferation during neurodevelopment. scRNA-seq on human cerebral organoids revealed a delayed transition of cell-type composition. Molecular characterization identified a group of differentially expressed genes (DEGs) that were temporally regulated by ALK at distinct developmental stages. In addition to oncogenes, many DEGs found by scRNA-seq are associated with neurological or neuropsychiatric disorders. Our study demonstrates a pivotal role of oncogenic ALK pathway in neurodevelopment and characterized cell-type-specific transcriptome regulated by ALK for better understanding mammalian cortical development.
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http://dx.doi.org/10.1093/cercor/bhab058DOI Listing
April 2021

Dimethyl Fumarate Induces Metabolic Crisie to Suppress Pancreatic Carcinoma.

Front Pharmacol 2021 22;12:617714. Epub 2021 Feb 22.

Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

Dimethyl fumarate (DMF) is an approved drug used in the treatment of multiple sclerosis (MS) and psoriasis therapy. Multiple studies have demonstrated other pharmacological activities of DMF such as an anti-cancer agent. In particular, studies have shown that DMF can modulate the NRF2/HO1/NQO1 antioxidant signal pathway and inactivate NF-κB to suppress the growth of colon and breast cancer cells, and induce cell death. In this study, we aimed to evaluate the anti-tumor activities of DMF in pancreatic cancer (PC) focusing on cell death as the predominant mechanism of response. We showed that both mitochondrial respiration and aerobic glycolysis were severely depressed following treatment with DMF and the effects could be abrogated by treatment with L-cysteine and N-acetyl-L-cysteine (NAC). Importantly, we verified that DMF induced metabolic crisis and that cell death was not related to alterations in ROS. Our data implied that MTHFD1 could be a potential downstream target of DMF identified by molecular docking analysis. Finally, we confirmed that MTHFD1 is up-regulated in PC and overexpression of MTHFD1 was negatively related to outcomes of PC patients. Our data indicate that DMF induces metabolic crisie to suppress cell growth and could be a potential novel therapy in the treatment of PC.
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http://dx.doi.org/10.3389/fphar.2021.617714DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7937954PMC
February 2021

Comparison of fibrosis regression of entecavir alone or combined with pegylated interferon alpha2a in patients with chronic hepatitis B.

Hepatol Int 2021 Mar 7. Epub 2021 Mar 7.

Liver Research Center, Beijing Key Laboratory of Translational Medicine On Liver Cirrhosis, Beijing Friendship Hospital, National Clinical Research Center of Digestive Diseases, Capital Medical University, 95 Yong-an Road, Xi-Cheng District, Beijing, 100050, China.

Background And Aims: Antiviral treatment with necleos(t)ide analogues contributes to histological improvement and virologic response in chronic hepatitis B (CHB) patients. However, whether adding pegylated interferon alpha2a (Peg-IFN-α-2a) can help additional clinical benefit, particularly on fibrosis regression was still unknown.

Methods: Chronic hepatitis B patients with pre-treatment biopsy-proven Ishak fibrosis score 2, 3 or 4 were randomly assigned to entecavir (ETV) alone or ETV plus Peg-IFN-α-2a (Peg-IFN-α-2a add-on) group (1:2 ratio). Post-treatment liver biopsy was performed at week 78. Fibrosis regression was defined as decrease in Ishak fibrosis score by ≥ 1 stage or predominantly regressive categorized by P-I-R score. Serum HBV DNA levels were assessed at baseline and every 26 weeks, while HBsAg and HBeAg were evaluated at baseline and every 52 weeks.

Results: A total of 218 treatment-naive CHB patients were randomly assigned to ETV alone or Peg-IFN-α-2a add-on group. Totals of 155 patients (ETV alone: Peg-IFN-α-2a add-on, 47:108) were included in statistical analysis. Fibrosis regression rates were 68% (32/47) in the ETV alone and 56% (60/108) in Peg-IFN-α-2a add-on group (p = 0.144). Both groups showed a similar trend of virological suppression during the process of 104-week antiviral therapy (p = 0.132). HBeAg or HBsAg loss or seroconversion rates in the ETV alone group were lower than Peg-IFN-α-2a add-on group though without statistical significance.

Conclusions: Peg-IFN-α-2a add-on therapy did not yield additional fibrosis regression and virologic response than ETV alone therapy.
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http://dx.doi.org/10.1007/s12072-021-10162-1DOI Listing
March 2021

Hepatocellular Carcinoma Prediction Models in Chronic Hepatitis B: A Systematic Review of 14 Models and External Validation.

Clin Gastroenterol Hepatol 2021 Mar 3. Epub 2021 Mar 3.

National Clinical Research Center of Digestive Diseases, Beijing Friendship Hospital, Capital Medical University, Beijing, Mainland China; Genomics Research Center, Academia Sinica, Taipei, Taiwan; Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan; Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan. Electronic address:

Background & Aims: The aim of our study was to characterize the performance of hepatocellular carcinoma (HCC) prediction models in chronic hepatitis B (CHB) patients through meta-analysis followed by external validation.

Methods: We performed a systematic review and meta-analysis of current literature, followed by external validation in independent multi-center cohort with 986 patients with CHB undergoing entecavir treatment (median follow-up: 4.7 years). Model performance to predict HCC within 3, 5, 7, and 10 years was assessed using area under receiver operating characteristic curve (AUROC) and calibration index. Subgroup analysis were conducted by treatment status, cirrhotic, race and baseline alanine aminotransferase.

Results: We identified 14 models with 123,885 patients (5,452 HCC cases), with REACH-B, CU-HCC, GAG-HCC, PAGE-B and mPAGE-B models being broadly externally validated. Discrimination was generally acceptable for all models, with pooled AUC ranging from 0.70 (95% CI, 0.63-0.76 for REACH-B) to 0.83 (95% CI, 0.78-0.87 for REAL-B) for 3-year, 0.68 (95% CI, 0.64-0.73 for REACH-B) to 0.81 (95% CI, 0.77-0.85 for REAL-B) for 5-year and 0.70 (95% CI, 0.58-0.80 for PAGE-B) to 0.81 (95% CI, 0.78-0.84 for REAL-B and 0.77-0.86 for AASL-HCC) for 10-year prediction. However, calibration performance was poorly reported in most studies. In external validation cohort, REAL-B showed highest discrimination with 0.76 (95% CI, 0.69-0.83) and 0.75 (95% CI, 0.70-0.81) for 3 and 5-year prediction. The REAL-B model was also well calibrated in the external validation cohort (3-year Brier score 0.066). Results were consistent in subgroup analyses.

Conclusions: In a systematic review of available HCC models, the REAL-B model exhibited best discrimination and calibration.
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http://dx.doi.org/10.1016/j.cgh.2021.02.040DOI Listing
March 2021

Role of Mutations in the Resistance to Aminoglycosides and β-Lactams in serovar Typhimurium.

Front Microbiol 2021 4;12:604079. Epub 2021 Feb 4.

State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.

Although it has been reported that deletion of the response regulator, CpxR, in the CpxRA system confers sensitivity to aminoglycosides (AGAs) and β-lactams in serovar Typhimurium, the regulatory effects of CpxA on multidrug resistance (MDR) are yet to be fully investigated in this organism. Here, to explore the role of CpxA in MDR, various mutants including a null mutant (JSΔ), a site-directed mutant (JSΔ) and an internal in-frame deletion mutant (JSΔ ) of the serovar Typhimurium strain JS, were constructed. It was revealed that and deletion mutants have opposing roles in the regulation of resistance to AGAs and β-lactams. Amikacin and cefuroxime can activate the CpxRA system, which results in increased resistance of the wild-type compared with the deletion mutant. All the mutations significantly increased resistance to AGAs and β-lactams due to CpxRA system activation the phosphorylation of CpxR. Moreover, AckA-Pta-dependent activation of CpxR increased the antibiotic resistance of deletion mutants. Further research revealed that the AcrAB-TolC conferred resistance to some AGAs and β-lactams but does not influence the regulation of resistance by CpxRA against these antibiotics. The detection of candidate MDR-related CpxR regulons revealed that the mRNA expression levels of , , , , , and were upregulated and that of was downregulated in various mutants. Furthermore, the expression levels of and mRNAs were downregulated only in JSΔ . These results suggested that mutations contribute to AGAs and β-lactams resistance, which is dependent on CpxR.
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http://dx.doi.org/10.3389/fmicb.2021.604079DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889517PMC
February 2021

Silver nanoparticle driven signal amplification for electrochemical chiral discrimination of amino acids.

Analyst 2021 Mar 19;146(5):1612-1619. Epub 2021 Feb 19.

Jiangsu Key Laboratory of Advanced Materials and Technology, Changzhou University, Changzhou 213164, China.

β-Cyclodextrin (β-CD) modified silver nanoparticles (AgNPs), denoted as β-CD/AgNPs, were prepared by a simple one-pot method. Due to the inherent chirality of β-CD, the developed β-CD/AgNPs exhibited higher affinity toward l-tyrosine (l-Tyr) than d-tyrosine (d-Tyr), leading to serious aggregation of AgNPs in the presence of l-Tyr. Consequently, the l-Tyr induced aggregation of AgNPs can result in signal amplification in the differential pulse voltammograms (DPVs) of l-Tyr, which can be applied for the electrochemical chiral discrimination of the Tyr enantiomers. Other chiral amino acids including tryptophan and phenylalanine can also be successfully discriminated with the β-CD/AgNPs, suggesting high universality of the developed chiral sensor.
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http://dx.doi.org/10.1039/d1an00119aDOI Listing
March 2021

Effect of Strain-Specific Biofilm Properties on the Retention of Colloids in Saturated Porous Media under Conditions of Stormwater Biofiltration.

Environ Sci Technol 2021 02 1;55(4):2585-2596. Epub 2021 Feb 1.

Department of Environmental Health and Engineering, Johns Hopkins University, 3400 N. Charles Street, Baltimore, Maryland 21218, United States.

Filter performance can be affected by bacterial colonization of the filtration media, yet little is known about how naturally occurring bacteria modify the surface properties of filtration media to affect colloidal removal. We used sand columns and simulated stormwater conditions to study the retention of model colloidal particles, carboxyl-modified-latex (CML) beads, in porous media colonized by naturally occurring bacterial strains. Colloid retention varied substantially across identical columns colonized by different, in some cases closely related, bacterial strains in a cell density independent manner. Atomic force microscopy was applied to quantify the interaction energy between CML beads and each bacterial strain's biofilm surface. We found interaction energy between CML and each strain was significantly different, with adhesive energies between the biofilm and CML, presumed to be associated with polymer-surface bonding, a better predictor of CML retention than other strain characteristics. Overall, the findings suggest that interactions with biopolymers in naturally occurring bacterial biofilms strongly influence colloid retention in porous media. This work highlights the need for more investigation into the role of biofilm microbial community composition on colloid removal in porous media to improve biofilter design and operation.
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http://dx.doi.org/10.1021/acs.est.0c06177DOI Listing
February 2021

Effects of Acute Visual Stimulation Exercise on Attention Processes: An ERP Study.

Int J Environ Res Public Health 2021 01 27;18(3). Epub 2021 Jan 27.

Division of Sport Science, Pusan National University, Busan 46241, Korea.

Backgrounds: It remains to be determined whether visual stimuli during exercise differentially influence the attention process. The purpose of the present study was to examine if different color stimuli during aerobic exercise are associated with different attention processes.

Methods: 22 college students completed a four 30-min running session during the presentation of different color stimuli (blue, green, red, and yellow) and without color stimulus on separate visits. The Kanizsa triangle task was administrated before and immediately after exercise to assess the attention process. Behavioral performance (accuracy and response time (RT)) and event-related potential (P2, N2b and P3a) were recorded during the test.

Results: Valid/invalid cue RT during the Kaniza test performance was significantly faster following the presentation of color stimuli during treadmill exercise compared to the seated rest. During exercise, these changes were larger after green and yellow stimuli than red in invalid cue RT. P2, N2b and P3a amplitudes of green were significantly larger than the other colors for both valid and invalid cues. Red color showed the lowest P2 and P3a amplitudes for both valid and invalid cues among colors.

Conclusion: The distinctive neurocognitive changes during aerobic exercise suggest different effects of color stimuli on visual search attention, attention capture, attentional orienting and processing speed. This study will be a first step to understand the optimal environmental setting during exercise for subsequent improvements in the attention process.
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http://dx.doi.org/10.3390/ijerph18031107DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7908307PMC
January 2021

The risk factors for cognitive dysfunction in elderly patients after laparoscopic surgery: A retrospective analysis.

Medicine (Baltimore) 2021 Jan;100(2):e23977

Key Laboratory of Birth Defects and Related Diseases of Women and Children, West China Second Hospital of Sichuan University, China.

Abstract: Postoperative cognitive dysfunction (POCD) is very common in clinical settings, it is necessary to analyze the risk factors for POCD in elderly patients after laparoscopic surgery to provide insights into clinical surgery management.Elderly patients undergone laparoscopy between September 1, 2018 and March 31, 2020 were included. The mini-mental state examination (MMSE) scale was used to evaluate the POCD, and the characteristics and clinical data of patients with and without POCD were collected and compared. Logistic regression was used to analyze the potential influencing factors.A total of 572 patients with laparoscopic surgery were included. The incidence of POCD was 11.89%. There were significant differences in the history of cerebral infarction, preemptive analgesia, preoperative use of dexmedetomidine, general anesthesia combined with continuous epidural block, duration of surgery, low SpO2 during anesthesia induction, PaCO2 after pneumoperitoneum, postoperative PCEA and VAS score at the third day after operation (all P < .05). Logistic regression analysis revealed that history of cerebral infarction (OR3.12, 1.02∼5.13), low SpO2 during anesthesia induction (OR2.03, 1.19∼4.47), Longer duration of surgery (OR1.82, 1.01∼3.16) were risk factors for POCD in elderly patients with laparoscopic surgery, while postoperative PCEA (OR0.43, 0.01∼0.91), General anesthesia combined with continuous epidural block (OR0.59, 0.04∼0.87), preoperative use of dexmedetomidine (OR0.70, 0.08∼0.94) and preemptive analgesia (OR0.75, 0.13-0.90) were the protective factors for POCD in elderly patients with laparoscopic surgery.For the elderly patients undergoing laparoscopic surgery, the health care providers should be fully alert to the POCD based on those relevant factors.
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http://dx.doi.org/10.1097/MD.0000000000023977DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7808459PMC
January 2021

Cell cycle during neuronal migration and neocortical lamination.

Int J Dev Neurosci 2021 Apr 24;81(2):209-219. Epub 2021 Jan 24.

National Health Commission, Key Laboratory of Birth Defect Prevention, Henan Scientific and Technical Institute of Reproductive Health, Zhengzhou, China.

Objectives: In order to understand the relationships between neocortical lamination and cell cycle, various cells, such as neural stem cell, migrating postmitotic neuron, Cajal-Retzius (CR) cell, and mature pyramidal cell in various cell phases were investigated in mouse cortices.

Methods: With mouse neocortex and hippocampus, the immunofluorescent labeling, BrdU assay, and DiI tracing technique were implemented in the study.

Results: (1) During mouse development, the neocortex expressed different proteins, such as FOXP2, CDP, and Nestin, which could be used as the markers for cortical lamination. (2) The neural stem cells were mainly located in the subventricular zone, with the expressions of Nestin, Cyclin A2, Cyclin E1, and CDT1, suggesting that they were in the repeated cell cycle. Furthermore, the migrating neurons in the neocortex were Cyclin D1- (G1 phase-specific marker) positive, suggesting that they were in the G1 phase. However, Pyramidal cells that developed from postmitotic migrating neurons and settled in the cortical plate were Cyclin D1- negative, suggesting that they were in the G0 phase. (3) Reelin positive CR cells appeared in the molecular layer of the neocortex in early embryonic day (E10), which could express Cyclin A2, Cyclin E1, and CDT1 as pyramidal cells, but not Cyclin D1, suggesting that they may have exited the cell cycle and entered the G0 phase.

Conclusion: The neural migration, neural proliferation, and cell cycle alterations play an important role during cortical lamination. During the cortical development and lamination, the neural stem cells and migrating postmitotic neurons are in different cell cycle phases, but pyramidal cells and CR cells have exited the cell cycle.
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http://dx.doi.org/10.1002/jdn.10091DOI Listing
April 2021

Oridonin interrupts cellular bioenergetics to suppress glioma cell growth by down-regulating PCK2.

Phytother Res 2021 Jan 13. Epub 2021 Jan 13.

Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research, Department of Neurosurgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

We aim to evaluate the tumor metabolic suppressive activity of Oridonin (extract of Rabdosia rubescens) in glioma and elucidate its potential mechanism. Effects of Oridonin on U251/U87 cells were determined by CCK8, RTCA, colony formation, flow cytometry, wound healing, and Transwell assay. Xenograft tumor model to evaluate the effect of Oridonin on glioma cells in vivo. Cellular bioenergetics were measured by Seahorse. RNA-seq was performed to screen potential biological pathways in Oridonin treated cells. Bioinformatics analysis of PCK2 in glioma was performed based on TCGA/CGGA. Endogenous PCK2 was knocked-down by lentivirus packaged shRNA. We found Oridonin significantly inhibited cell growth in U251/U87 in vitro and in vivo. Both oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were decreased in Oridonin-treated U251/U87 cells. Oridonin treatment led to PCK2 down-regulation. Additionally, PCK2 was up-regulated in higher grade glioma and correlated with poor outcomes. Furthermore, PCK2 depletion significantly inhibited cell growth and decreased OCR/ECAR in U251/U87 which coincided with the effects of Oridonin. Therefore, we evaluated the potent anti-tumor property of Oridonin in glioma. Importantly, we demonstrated that PCK2 might be a novel target of Oridonin on glioma by inducing energy crisis and increasing oxidative stress.
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http://dx.doi.org/10.1002/ptr.7009DOI Listing
January 2021

Sedimentary record of nutrients and sources of organic matter in the Shuanglong reservoir, Dianchi watershed, China.

Environ Sci Pollut Res Int 2021 Apr 5;28(14):17763-17774. Epub 2021 Jan 5.

School of Resources and Environmental Engineering, Shandong University of Technology, Zibo, 255049, Shandong, China.

In the last few decades, the eutrophication of lakes has been a serious issue in the middle and lower reaches of the Yangtze River watershed. To explore the relationship between lake systems and anthropogenic activities, sediments were collected from the Shuanglong reservoir in the Dianchi watershed in Southwest China. Total nitrogen (TN), total phosphorus (TP), total organic carbon (TOC), and the carbon isotopic ratio (δC) were analyzed in sediment cores to reconstruct the effects of natural succession and human activities on the past lacustrine environmental conditions. A reliable chronology of the sediment core was established by using the Pb dating technique, which indicated that the age span of the 70-cm sediment core is from the years 1871 to 2011. Above - 31 cm depth in the core, TN, TP, TOC, C/N, and δC increased significantly, indicating that eutrophication has occurred since the 1980s. By combining the indicators of δC and C/N, it was shown that terrestrial and lacustrine components were the main sources of organic matter (OM) in the reservoir, which was mostly controlled by terrestrial C plants and algae. Since the 1980s, increased sewage discharge, fish aquaculture, fertilizer application, population, and economic strength have sped up the eutrophication process, and the eutrophication was further intensified in 2001.
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http://dx.doi.org/10.1007/s11356-020-12085-0DOI Listing
April 2021

Fine Customization of Calcium Phosphate Nanostructures with Site-Specific Modification by DNA Templated Mineralization.

ACS Nano 2021 01 17;15(1):1555-1565. Epub 2020 Dec 17.

Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, 1037 Luoyu Load, Wuhan 430074, China.

Calcium phosphate (Ca-P) is the most abundant biomineral in hard tissues with diverse microstructures, which in nature ensure a broad range of functionalities with virtually similar and simple chemical compositions. Artificial fabrication of rationally designed Ca-P materials with arbitrary microstructures is a long-standing challenge for inorganic chemists. Although DNA nanotechnology has been elegantly used to modulate the nanofabrication of inorganic materials because of its programmability, encoding customized Ca-P mineralization with high structural precision remains unachievable because of fast affinity-driven crystal growth. Herein, this long-standing ambition has been skillfully fulfilled by taking advantage of crystallization via a particle attachment (CPA) process. The derived hybrid materials not only well inherited the structural details encoded by the DNA template but also exhibited significantly enhanced mechanical strength, even after heating. Moreover, this method preserved preinstalled synthetic functionalities on the DNA surface, allowing for downstream site-specific modification.
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http://dx.doi.org/10.1021/acsnano.0c08998DOI Listing
January 2021

Insight into the heavy metal binding properties of dissolved organic matter in mine water affected by water-rock interaction of coal seam goaf.

Chemosphere 2021 Feb 28;265:129134. Epub 2020 Nov 28.

School of Chemical & Environment Engineering, China University of Mining and Technology (Beijing), Beijing, 100083, China.

The water-rock interaction has a significant effect on the binding characteristics of dissolved organic matter and heavy metals when mine water flows in goaf. This study used fluorescence excitation-emission matrix (EEM) quenching combined with parallel factor (PARAFAC) analysis to characterize the binding properties of DOM with Fe (Ⅲ), Fe (Ⅱ+Ⅲ) and Mn (Ⅱ) in mine water under rock-influenced conditions. Two protein-like components and two humic-like components were identified by PARAFAC, in which protein-like components dominated (75.9%). The fluorescence intensity of each component can all be weakened, especially the stability constant (logK) value of Fe (Ⅱ+Ⅲ) with fulvic-like acid and humic-like acid was the largest and the binding was more stable. Clay minerals and iron-bearing minerals in rocks had significant effects on the binding characteristics of DOM and metal ions under water-rock interaction. Iron ions released by the oxidation of pyrite and siderite in sandstone can reduce the fluorescence intensity of the derived components. The competitive adsorption effect of clay minerals on metal ions made the fluorescence intensity of the derived components under the action of sandstone containing less clay minerals (19.5%) be lower than that of mudstone (31.3%). Meanwhile, the process of water-rock interaction was accompanied by microbial activities to convert protein-like components into fulvic-like and humic-like components, or higher levels of stable substances. This study shows that when assessing the potential ability of mine water DOM and metal ions binding and migration during the flow of water in goaf it is crucial to take into account the presence of water-rock interaction.
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http://dx.doi.org/10.1016/j.chemosphere.2020.129134DOI Listing
February 2021

Screening varices in patients with HBV-related cirrhosis on antiviral therapy: Platelet alone or together with LSM.

Liver Int 2021 02 18;41(2):369-377. Epub 2020 Dec 18.

Liver Research Center, Beijing Friendship Hospital, Capital Medical University, Beijing Key Laboratory of Translational Medicine on Liver Cirrhosis, National Clinical Research Center for Digestive Diseases, Beijing, China.

Background & Aims: Non-invasive assessment criteria to rule out high-risk varices (HRV) in compensated hepatitis B virus (HBV) cirrhosis on antiviral therapy remains unclear.

Methods: HBV-related compensated cirrhotic patients who underwent screening endoscopy during antiviral therapy were enrolled and randomly divided into the derivation and validation sets. HRV were defined as medium to large varices or small varices with red signs. Univariate and multivariate logistic analysis were used to determine the parameters associated with HRV.

Results: A total of 436 HBV-related compensated cirrhotic patients screened for varices were enrolled, the median duration of antiviral therapy was 4 years (IQR: 2.5-5.5 years). In the derivation set (N = 290, 17.2% with HRV), only platelet (PLT) count (OR = 0.972, 95% CI 0.961-0.984, P < .05) was independently associated with HRV, whereas liver stiffness measurement was not associated with the presence of HRV. With a PLT count cut-off value of 105 × 10 /L, unnecessary endoscopies could be spared in 56.9% patients, with a 3.6%. risk of missing HRV. In the validation cohort (N = 146, 16.4% with HRV), the proportion of patients that could safely spare endoscopies (61.0%) identified by this PLT count cut-off value was higher than that obtained by using Baveno VI criteria (34.9%), with an acceptable risk of missing HRV (3.4%).

Conclusion: Compared with the 'Baveno VI criteria or beyond' criteria, PLT count higher than 105 × 10 /L could safely spare more screening endoscopies without increasing the risk of missing HRV in patients with HBV-related compensated cirrhosis on antiviral therapy.
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http://dx.doi.org/10.1111/liv.14752DOI Listing
February 2021

Surface dual redox cycles of Mn(III)/Mn(IV) and Cu(I)/Cu(II) for heterogeneous peroxymonosulfate activation to degrade diclofenac: Performance, mechanism and toxicity assessment.

J Hazard Mater 2021 May 18;410:124623. Epub 2020 Nov 18.

Guangdong Key Laboratory of Environmental Pollution and Health, School of Environment, Jinan University, Guangzhou 511443, PR China. Electronic address:

Advanced oxidation processes (AOPs) based on heterogeneous catalytic activated peroxymonosulfate (PMS) have been becoming alternatives to conventional wastewater treatment technologies to directly degrade chemical contaminants. To build dual/multi redox cycles of different metal ions may be an effective means for better PMS activation. Herein, this study designed MnO/CuBiO with dual redox cycles of Mn(III)/Mn(IV) and Cu(I)/Cu(II) to activate PMS for efficiently decomposing and mineralizing diclofenac sodium (DCF). Under optimal reaction conditions, DCF (50 mg/L) was degraded totally within 10 min, and TOC removal rate reached up to 74.3%. The possible mechanism of PMS activation by MnO/CuBiO was proposed, wherein dual redox cycles of Mn(III)/Mn(IV) and Cu(I)/Cu(II) on MnO/CuBiO effectively facilitated PMS activation to generate ·O, O, SO· and ·OH, which was responsible for DCF degradation. Moreover, combined with degraded products detected by high resolution liquid chromatography coupled to mass spectrometry and corresponding toxic assessment results, the possible degradation pathways of DCF were proposed and the relative toxicity of degraded products was evaluated. This work may be useful for developing stronger heterogeneous activators of PMS to construct more efficient AOPs for purifying wastewater.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124623DOI Listing
May 2021

Drainage smell and peritonitis are efficient indicators of anastomotic leakage after laparoscopic rectal cancer resection.

J Int Med Res 2020 Nov;48(11):300060520970766

Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing Key Laboratory of Cancer Invasion and Metastasis Research & National Clinical Research Center for Digestive Diseases, Beijing, P.R. China.

Objective: Anastomotic leakage (AL) is a frequent complication after laparoscopic rectal cancer resection (LRCR). The main objective of the present study was to identify accurate indicators of AL after LRCR.

Methods: A retrospective case-control study was performed of 185 patients who underwent laparoscopic surgery for rectal cancer between March 2012 and February 2017 at Beijing Friendship Hospital. Potential indicators of AL were examined via univariate and multivariate analyses. The performance of multivariate analysis was evaluated using receiver operating characteristic (ROC) curves.

Results: The overall AL rate was 17.84%. Multivariate analysis identified drainage smell (odds ratio [OR = 35.318, 95% confidence interval [CI] = 7.114 to 175.338) and peritonitis [OR = 17.475, 95% CI = 1.540 to 198.318) as independent indicators of AL. The area under the ROC curve was 0.720 (95% CI = 0.606 to 0.835).

Conclusion: Drainage smell and peritonitis could be reliable and accurate indicators of AL after LRCR.
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http://dx.doi.org/10.1177/0300060520970766DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7686604PMC
November 2020

J-curve relationship between admission SBP and 2-year cardiovascular mortality in older patients admitted for acute coronary syndrome.

J Hypertens 2021 May;39(5):926-934

Department of Internal Medicine and Geriatrics.

Objective: To investigate the relationship between admission SBP and subsequent cardiovascular and all-cause mortality in older patients hospitalized for acute coronary syndrome (ACS).

Methods: This is a retrospective observational study. Data from the CBD Bank (Cardiovascular Center Beijing Friendship Hospital Database Bank) were used to analyze the cardiovascular and all-cause mortality during hospitalization and over the follow-up period in relation to admission SBP among patients aged at least 65 years admitted for ACS from December 2012 through July 2019. Results were presented according to SBP quartiles: Q1, less than 120 mmHg; Q2, from 120 to 129 mmHg; Q3, from 130 to 143 mmHg; and Q4, at or above 144 mmHg.

Results: A total of 6785 patients were included in this cohort study. Mean (SD) patient age was 74.0 (6.5) years, and 47.6% were women. Mean (SD) follow-up time was 2.54 (1.82) years. A nonlinear relation was observed between SBP at admission and cardiovascular and all-cause mortality during hospitalization and over the follow-up period using restricted cubic splines. After adjustment for potential confounders, patients in Q1 had higher risk for 2-year cardiovascular death by Cox proportional hazard model compared with patients in Q2 [hazard ratio, 1.58; 95% confidence interval (CI), 1.12-2.21, P = 0.009], whereas patients in Q3 or Q4 exhibited a trend towards increased risk for 2-year cardiovascular death (hazard ratio, 1.33, 95% CI, 0.95-1.86, P = 0.094, for Q3 vs. Q2; and hazard ratio, 1.28, 95% CI, 0.91-1.82, P = 0.160, for Q4 vs. Q2). Meanwhile, when compared with patients in Q1, patients in Q2 had lower risk for 2-year cardiovascular death (hazard ratio, 0.64; 95% CI, 0.45-0.89, P = 0.009) whereas patients in Q3 or Q4 had similar risk for cardiovascular death (hazard ratio, 0.85, 95% CI, 0.63-1.14, P = 0.272, for Q3 vs. Q1; and hazard ratio, 0.82, 95% CI, 0.59-1.13, P = 0.221, for Q4 vs. Q1). However, low-admission SBP was not an independent predictor of 2-year all-cause mortality in this population.

Conclusion: Among patients aged at least 65 years admitted for ACS, there is a J-curve relationship between supine admission SBP and risk for 2-year cardiovascular death, with a nadir at 120-129 mmHg.
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http://dx.doi.org/10.1097/HJH.0000000000002737DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8048722PMC
May 2021

J-curve relationship between admission SBP and 2-year cardiovascular mortality in older patients admitted for acute coronary syndrome.

J Hypertens 2021 May;39(5):926-934

Department of Internal Medicine and Geriatrics.

Objective: To investigate the relationship between admission SBP and subsequent cardiovascular and all-cause mortality in older patients hospitalized for acute coronary syndrome (ACS).

Methods: This is a retrospective observational study. Data from the CBD Bank (Cardiovascular Center Beijing Friendship Hospital Database Bank) were used to analyze the cardiovascular and all-cause mortality during hospitalization and over the follow-up period in relation to admission SBP among patients aged at least 65 years admitted for ACS from December 2012 through July 2019. Results were presented according to SBP quartiles: Q1, less than 120 mmHg; Q2, from 120 to 129 mmHg; Q3, from 130 to 143 mmHg; and Q4, at or above 144 mmHg.

Results: A total of 6785 patients were included in this cohort study. Mean (SD) patient age was 74.0 (6.5) years, and 47.6% were women. Mean (SD) follow-up time was 2.54 (1.82) years. A nonlinear relation was observed between SBP at admission and cardiovascular and all-cause mortality during hospitalization and over the follow-up period using restricted cubic splines. After adjustment for potential confounders, patients in Q1 had higher risk for 2-year cardiovascular death by Cox proportional hazard model compared with patients in Q2 [hazard ratio, 1.58; 95% confidence interval (CI), 1.12-2.21, P = 0.009], whereas patients in Q3 or Q4 exhibited a trend towards increased risk for 2-year cardiovascular death (hazard ratio, 1.33, 95% CI, 0.95-1.86, P = 0.094, for Q3 vs. Q2; and hazard ratio, 1.28, 95% CI, 0.91-1.82, P = 0.160, for Q4 vs. Q2). Meanwhile, when compared with patients in Q1, patients in Q2 had lower risk for 2-year cardiovascular death (hazard ratio, 0.64; 95% CI, 0.45-0.89, P = 0.009) whereas patients in Q3 or Q4 had similar risk for cardiovascular death (hazard ratio, 0.85, 95% CI, 0.63-1.14, P = 0.272, for Q3 vs. Q1; and hazard ratio, 0.82, 95% CI, 0.59-1.13, P = 0.221, for Q4 vs. Q1). However, low-admission SBP was not an independent predictor of 2-year all-cause mortality in this population.

Conclusion: Among patients aged at least 65 years admitted for ACS, there is a J-curve relationship between supine admission SBP and risk for 2-year cardiovascular death, with a nadir at 120-129 mmHg.
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http://dx.doi.org/10.1097/HJH.0000000000002737DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8048722PMC
May 2021

Loose combined cutting seton for patients with high intersphincteric fistula: a retrospective study.

Ann Transl Med 2020 Oct;8(19):1236

Qihuang Class of 2017, Beijing University of Chinese Medicine, Beijing, China.

Background: Achieving a complete cure while maintaining continence constitutes a considerable challenge in the treatment of patients with high anal fistula. This study aimed to evaluate the effectiveness of loose combined cutting seton (LCCS) for treating patients with high intersphincteric fistula.

Methods: Consecutive patients with high intersphincteric fistula who underwent LCCS were retrospectively enrolled. Patient data including demographics, medical history, comorbidities, details of the fistula, operative procedure, and prognosis were collected. Postoperative pain was assessed using a visual analog scale (VAS), which ranged from 0 (no pain) to 10 (extremely severe pain). The severity of fecal incontinence was assessed using the Wexner Continence Grading Scale, with a total score ranging from 0 (no incontinence) to 20 (complete incontinence). The primary outcome was the healing rate of fistula. Secondary outcomes included the recurrence rate of fistula and the severity of fecal incontinence.

Results: The 22 patients (male: female =18:4) in our study had a median follow-up of 55 (range, 32-568) days. The healing rate was 100%, and none of the patients experienced fistula recurrence. At the follow-up visit, 19 patients (86.4%) reported no fecal incontinence. The median total Wexner score was 0. 95.5% patients had VAS score of 0 and only 1 patient (4.5%) had a VAS score of 1, which indicated a low level of postoperative pain.

Conclusions: LCCS achieved a high healing rate with an increased level of continence, as well as a low level of postoperative pain, in most patients with high anal fistula in our study. Further randomized controlled trials are needed to confirm the effectiveness of this novel seton-based technique.
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http://dx.doi.org/10.21037/atm-20-6123DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7607110PMC
October 2020

Circular RNA HIPK3 plays a carcinogenic role in cervical cancer progression via regulating miR-485-3p/FGF2 axis.

J Investig Med 2021 Mar 11;69(3):768-774. Epub 2020 Nov 11.

Obstetrics and Gynecology, Linyi Central Hospital, Linyi, Shandong, China

Circular RNA (circRNA) is an endogenous RNA molecule with a stable closed-loop structure. The circular RNA HIPK3 (circHIPK3) is highly expressed in hepatocellular carcinoma and facilitates tumor growth. However, its role in cervical cancer (CC) and its regulatory mechanisms are not well-studied. This study aimed for investigating the function of circHIPK3 on proliferation and metastasis of CC cells. In this study, quantitative real-time PCR assay was adopted to delve into the circHIPK3 expression in CC cell lines. Cell counting kit-8 and colony formation assays were used to evaluate the influence of overexpression and knockdown of circHIPK3 on CC cell proliferation. Dual-luciferase reporter assay was employed to probe into the binding of miR-485-3p to circHIPK3 and miR-485-3p to the 3' untranslated region (UTR) of fibroblast growth factor 2 (FGF2), respectively. FGF2 protein expression was detected by western blot analysis. This study confirmed that circHIPK3 was highly expressed in CC tissues. Overexpressed circHIPK3 could remarkably expedite the proliferation, migration and invasion of SiHa cells, and knocking down circHIPK3 could significantly impede the proliferation, migration and invasion of HeLa cells. MiR-485-3p can directly bind to circHIPK3 and the 3'UTR of FGF2. Overexpression of circHIPK3 triggered the upregulation of FGF2 expression while knockdown of circHIPK3 reduced FGF2 expression in CC cells, and the transfection of miR-485-3p mimics reversed the upregulation of FGF2 expression and enhanced malignant phenotypes in CC cells with overexpressed circHIPK3.
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http://dx.doi.org/10.1136/jim-2020-001537DOI Listing
March 2021

Comments on "Prognostic Value of the Pretreatment Primary Lesion Quantitative Dynamic Contrast-Enhanced Magnetic Resonance Imaging for Nasopharyngeal Carcinoma".

Acad Radiol 2020 12 7;27(12):1794. Epub 2020 Nov 7.

The Second Affiliated Hospital of Guangzhou University of Chinese Medicine & Guangdong Provincial Hospital of Chinese Medicine, 111 Da De Lu, Guangzhou, Guangdong Province 510120, P.R. China. Electronic address:

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http://dx.doi.org/10.1016/j.acra.2020.08.019DOI Listing
December 2020

Efficacy and safety of rituximab for systemic lupus erythematosus treatment: a meta-analysis.

Afr Health Sci 2020 Jun;20(2):871-884

Clinical Laboratory Department, The Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, No. 33 Beiling Street, Huanggu District, Shenyang, 110032, Liaoning, China.

Background: Given the inconsistency of previous studies and the newly emerging evidence, we decided to conduct a meta-analysis.

Methods: The meta-analysis included 2 randomized controlled trials and 13 observational studies 742 patients in total. Qualified studies were properly searched from databases . Data were analyzed by the RevMan 5.3 software. Results were demonstrated as WMD , SMD and RR with 95% CIs, I and P value.

Results: we observed that a remarkable increase of complement C3 in the rituximab group than placebo group (WMDfixed= 7.67mg/dL, 95%CIs=-0.16~15.50, I=0%, P=0.05). A significant increase of complement C4 was observed in the rituximab group than placebo group (WMDfixed=3.14mg/dL, 95%CIs=1.06~5.22, I=0%, P=0.003). Notably decreased peripheral CD19+B cells in rituximab group than placebo group (WMDfixed=-117.93n/µl, 95%CIs=-172.94~-62.91, I=0%, P<0.0001) in RCTs. Patients with severe or refractory SLE got more satisfactory efficacy results after receiving rituximab in observational studies, such as British Isles Lupus Assessment Group index score, SLE Disease Activity Index score, complement C3/C4, anti-dsDNA antibodies, peripheral CD19B cells and so on. Safety profiles were no difference between rituximab and placebo groups.

Conclusion: although the efficacy of rituximab is highly controversial for SLE, our study shows that rituximab presents a satisfying efficacy and safety for SLE.
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http://dx.doi.org/10.4314/ahs.v20i2.41DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7609121PMC
June 2020

Supplementation of Bacillus sp. DU-106 reduces hypercholesterolemia and ameliorates gut dysbiosis in high-fat diet rats.

Appl Microbiol Biotechnol 2021 Jan 31;105(1):287-299. Epub 2020 Oct 31.

College of Food Science, South China Agricultural University, Wushan Road 483, Tianhe District, Guangzhou, 510642, China.

Gut microbiota modulation by a probiotic is a novel therapy for hypercholesterolemia mitigation. This study initially investigated the potential hypocholesterolemic effect of Bacillus sp. DU-106 in hypercholesterolemic rats and explored its potential relation with gut microbiota. Sprague-Dawley rats received a high-fat diet, or a high-fat diet supplemented with 7.5 × 10 and 1.5 × 10 CFU/kg bw/day Bacillus sp. DU-106 (low-dose and high-dose groups). At the end of 9 weeks, Bacillus sp. DU-106 treatment significantly decreased the body weight, liver index, and total cholesterol. 16S rRNA sequencing showed that Bacillus sp. DU-106 intervention significantly increased bacterial richness and particularly increased the genus abundance of Turicibacter, Acinetobacter, Brevundimonas, and Bacillus and significantly decreased the abundance of Ralstonia. Metabolomic data further indicated that the supplementation of Bacillus sp. DU-106 remarkably changed the gut metabolic profiles of hypercholesterolemic rats and, in particular, elevated the metabolites of indole-3-acetate, methylsuccinic acid, creatine, glutamic acid, threonine, lysine, ascorbic acid, and pyridoxamine. Spearman's correlation analysis showed the close relation between the different genera and metabolites. In conclusion, Bacillus sp. DU-106 supplement ameliorated high-fat diet-induced hypercholesterolemia and showed potential probiotic benefits for the intestine. KEY POINTS: • A novel potential probiotic Bacillus sp. DU-106 ameliorated hypercholesterolemia in rats. • Bacillus sp. DU-106 supplement regulated gut microbiome structure and richness. • Bacillus sp. DU-106 supplement changed metabolic profiles in high-fat diet rats. • Significant correlations were observed between differential genera and metabolites.
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http://dx.doi.org/10.1007/s00253-020-10977-2DOI Listing
January 2021

Incomplete protective effect of coronary collateral circulation for acute myocardial infarction patients.

Medicine (Baltimore) 2020 Oct;99(43):e22750

Department of Cardiology, Beijing Friendship Hospital Affiliated with Capital Medical University, Beijing, China.

The short-term and long-term effects of coronary collateral circulation (CCC) discovered after acute myocardial infarction (AMI) are still debatable. This retrospective cohort study aimed to explore the clinical significance of CCC for AMI patients.A consecutive series of 323 AMI patients with CCC and 1339 AMI subjects without CCC were enrolled, most of them received percutaneous coronary intervention after AMI. Comparisons between CCC subjects and non-CCC population and between CCC sub-groups were applied regarded to basic clinical characteristics, stenosis extent indicated by Gensini score, myocardial infarction size estimated by peak concentration of troponin I (TnI), and left ventricular function evaluated by peak value of N-terminal pro-brain natriuretic peptide (NT-proBNP). Multiple linear regressions for NT-proBNP and TnI, and Kaplan-Meier curves for 5-years' main cardiovascular event (MACE) were also analyzed.CCC might provide incomplete protection by preventing excessive myocardial infarction but not a poorer heart function during AMI and CCC had no obvious protective effect on 5-years' MACE for AMI patients. More attentions should be paid to heart function for CCC patients during AMI.
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http://dx.doi.org/10.1097/MD.0000000000022750DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7581137PMC
October 2020

CT Radiomics in Predicting EGFR Mutation in Non-small Cell Lung Cancer: A Single Institutional Study.

Front Oncol 2020 7;10:542957. Epub 2020 Oct 7.

Department of Radiology, The Affiliated Hospital of Guizhou Medical University, Guiyang, China.

To evaluate the value of CT radiomics in predicting the epidermal growth factor receptor (EGFR) mutation of patients with non-small cell lung cancer (NSCLC), and combing with the clinical characteristic to construct the prediction model. Sixty-seven cases of NSCLC confirmed by pathology were enrolled. The pre-treatment chest CT enhanced images were used in Radiomics analysis. Two experienced radiologists delineated the region of interest (ROI) on open source software 3D-Slicer. The feature of ROI was extracted by Pyradiomics software package and a total of 849 features were extracted. By calculating Pearson correlation coefficient between pair-wise features and LASSO method for feature screening. The prediction model was constructed by logical regression, diagnostic efficacy of the model by the area under the receiver operating characteristic (ROC) curve was calculated. Based on clinical model and the radiomics model, the AUC under the ROC was 0.8387 and 0.8815, respectively. The model combining clinical and radiomics features perfect best, the AUC under the ROC was 0.9724, the sensitivity and specificity were 85.3 and 90.9%, respectively. Compared with clinical features or radiomics features alone, the model constructed by combining clinical and pre-treatment chest enhanced CT features may show more utility for improved patient stratification in EGFR mutation and EGFR wild.
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http://dx.doi.org/10.3389/fonc.2020.542957DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7576846PMC
October 2020

Development of MR-based preoperative nomograms predicting DNA copy number subtype in lower grade gliomas with prognostic implication.

Eur Radiol 2021 Apr 6;31(4):2094-2105. Epub 2020 Oct 6.

The Second Affiliated Hospital of Guangzhou University of Chinese Medicine & Guangdong Provincial Hospital of Chinese Medicine, 111 Da De Lu, Guangzhou, 510120, Guangdong Province, People's Republic of China.

Objectives: We aimed to determine the value of MR-based preoperative nomograms in predicting DNA copy number (CN) subtype in lower grade glioma (LGG) patients.

Methods: The overall survival (OS) data were analyzed. MRI data of 170 subjects were retrospectively analyzed. The correlation was explored by univariate and multivariate regression analysis.

Results: CN2 subtype was associated with shortest median OS (CN2 subtype vs. others: 46.8 vs. 221.7 months, p < 0.05). The time-dependent receiver operating characteristic for the CN2 subtype was 0.80 (95% CI: 0.74-0.85) for survival at 1 year, 0.80 (95% CI: 0.75-0.85) for survival at 2 years, and 0.77 (95% CI: 0.73-0.83) for survival at 3 years. On multivariate analysis, hemorrhage (OR: 0.118; p < 0.001; 95% CI: 0.037-0.376), poorly defined margin (OR: 4.592; p < 0.001; 95% CI: 1.965-10.730), extranodular growth (OR: 0.247; p = 0.006; 95% CI: 0.091-0.671), and volume ≥ 60 cm (OR: 4.734.256; p < 0.001; 95% CI: 2.051-10.924) were associated with CN1 subtype (AUC: 0.781). Proportion CE tumor (OR: 5.905; p = 0.007; 95% CI: 1.622-21.493), extranodular growth (OR: 9.047; p = 0.001; 95% CI: 2.349-34.846), width ≥ median (OR: 0.231; p = 0.049; 95% CI: 0.054-0.998), and depth ≥ median (OR: 0.192; p = 0.023; 95% CI: 0.046-0.799) were associated with CN2 subtype (AUC: 0.854). Necrosis/cystic (OR: 6.128; p = 0.007; 95% CI: 1.635-22.968), hemorrhage (OR: 5.752; p = 0.002; 95% CI: 1.953-16.942), poorly defined margin (OR: 0.164; p < 0.001; 95% CI: 0.063-0.427), and volume ≥ median (OR: 4.422; p < 0.001; 95% CI: 1.925-10.160) were associated with CN3 subtype (AUC: 0.808). All three nomograms showed good discrimination and calibration. Decision curve analysis supported that all nomograms were clinically useful. The average accuracy of the tenfold cross-validation was 0.680 (CN1), 0.794 (CN2), and 0.894 (CN3), respectively.

Conclusions: The shortest OS was observed in patients with CN2 subtype. This preliminary radiogenomics analysis revealed that the MR-based preoperative nomograms provide individualized prediction of DNA copy number subtype in LGG patients.

Key Points: • This preliminary radiogenomics analysis of LGG revealed that the MR-based preoperative nomograms provide individualized prediction of DNA copy number subtype in LGG patients. • The AUC for the ROC curve was 0.781 for CN1 subtype, 0.854 for CN2 subtype, and 0.808 for CN3 subtype. Decision curve analysis supported that all nomograms were clinically useful. • The sensitivity was 0.779 (CN1), 0.731 (CN2), and 0.851 (CN3), respectively. The specificity was 0.664 (CN1), 0.872 (CN2), and 0.625 (CN3), respectively. And the accuracy was 0.717 (CN1), 0.849 (CN2), and 0.692 (CN3), respectively.
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http://dx.doi.org/10.1007/s00330-020-07350-2DOI Listing
April 2021