Publications by authors named "Shanshan Liu"

629 Publications

Development and validation of the Professional Nursing Support Scale for Chinese patients with cancer.

Int J Nurs Pract 2021 Oct 17:e13020. Epub 2021 Oct 17.

Alice Centre for Nursing Studies, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.

Purpose: This study aimed to develop and test the psychometric properties of a Professional Nursing Support Scale for patients with cancer in mainland China.

Methods: The study was conducted in two phases from January 2017 to January 2020. Phase I involved item generation and developing a preliminary version of Professional Nursing Support Scale through literature review, qualitative interviews, content validity evaluation and a pilot study. Phase II involved psychometric properties testing of the Professional Nursing Support Scale in 700 patients with cancer recruited from four public hospitals in Anhui Province, China.

Results: A 49-item Professional Nursing Support Scale was finally developed. Exploratory factor analysis showed a four-factor structure of the 49-item Professional Nursing Support Scale, accounting for 56.95% of variance. Cronbach's α ranged from 0.91 to 0.94 for the four subscales. Confirmatory factor analysis further confirmed the best model fit of four-factor structure of the Professional Nursing Support Scale. The Professional Nursing Support Scale also showed an acceptable concurrent validity with Supportive Care Needs Scale short form 34 (r = 0.75, p < 0.001) and Psychological Distress Thermometer (r = 0.39, p < 0.001).

Conclusions: The Professional Nursing Support Scale is a reliable and valid instrument to assess perceived needs of professional nursing support and support acquisition in relation to the care in Chinese patients with cancer.
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http://dx.doi.org/10.1111/ijn.13020DOI Listing
October 2021

CHD1L augments autophagy-mediated migration of hepatocellular carcinoma through targeting ZKSCAN3.

Cell Death Dis 2021 Oct 15;12(10):950. Epub 2021 Oct 15.

Affiliated Cancer Hospital and Institute of Guangzhou Medical University, Guangzhou, Guangdong, China.

Autophagy is an important biological process in normal cells. However, how it affects tumor progression still remains poorly understood. Herein, we demonstrated that the oncogenic protein Chromodomain-helicase-DNA-binding-protein 1-like gene (CHD1L) might promote HCC cells migration and metastasis through autophagy. CHD1L could bind to the promotor region of Zinc finger with KRAB and SCAN domain 3 (ZKSCAN3), a pivotal autophagy suppressor, and inhibit its transcription. We established inducible CHD1L conditional knockout cell line (CHD1L-iKO cell) and found that the deletion of CHD1L significantly increased ZKSCAN3 expression both at mRNA and protein level. Deletion of CHD1L impaired the autophagic flux and migration of HCC cells, while specifically inhibiting ZKSCAN3 blocked these effects. Further exploration demonstrated that the enhanced tumor cell migration and metastasis induced by CHD1L was mediated through ZKSCAN3-induced autophagic degradation of Paxillin. In summary, we have characterized a previously unknown function of CHD1L in regulating tumor migration via ZKSCAN3-mediated autophagy in HCC. Further inhibition of CHD1L and its downstream autophagy signaling might shed new light on cancer therapeutics.
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http://dx.doi.org/10.1038/s41419-021-04254-xDOI Listing
October 2021

Genetic association between CDKN2B-AS1 polymorphisms and the susceptibility of primary open-angle glaucoma (POAG): a meta-analysis from 21,775 subjects.

Ir J Med Sci 2021 Oct 14. Epub 2021 Oct 14.

Department of Ophthalmology, The Fourth People's Hospital of Shenyang, Huanggu District, 20 Huanghe South Street, Shenyang, 11031, China.

Background: Primary open-angle glaucoma (POAG) is affected by both genetics and environmental factors. CDKN2B-AS1 polymorphisms have been reported to be involved in the pathogenesis of POAG. However, the results of the genetic associations between the CDKN2B-AS1 polymorphisms and POAG risk were inconclusive.

Aims: This study aimed to evaluate the correlation of CDKN2B-AS1 polymorphisms and POAG susceptibility using a meta-analysis.

Methods: Meta-analysis was performed by searching PubMed, Web of science, the Cochrane database of system reviews, CNKI, and Embase databases. The relationship of CDKN2B-AS1 rs4977756, rs10120688, rs2157719, and rs7049105 polymorphisms and POAG risk was evaluated by the odds ratios (ORs) and 95% confidence intervals (CIs).

Results: Eleven studies with 8290 cases and 13,485 controls were included in the present meta-analysis. The alleles of rs4977756 and rs10120688 significantly increased the risk of POAG (rs4977756: OR = 1.20, 95%CI = 1.03-1.39, p = 0.02; rs10120688: OR = 1.36, 95%CI = 1.29-1.44, p < 0.00001). As for ethnicity, rs4977756 polymorphism significantly increased POAG risk in Caucasians (OR = 1.33, 95%CI = 1.12-1.57, p = 0.0009), but not in Asians. In addition, the rs2157719 allele was significantly associated with POAG risk in Asians (OR = 0.66, 95%CI = 0.55-0.80, p < 0.0001), but not in Caucasians (p > 0.05).

Conclusions: The CDKN2B-AS1 rs4977756 might increase the POAG risk in Caucasian population, and rs2157719 might decrease the POAG risk in Asian population, while rs10120688 might increase the risk of POAG.
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http://dx.doi.org/10.1007/s11845-021-02794-xDOI Listing
October 2021

Can family doctor contracted services facilitate orderly visits in the referral system? A frontier policy study from Shanghai, China.

Int J Health Plann Manage 2021 Oct 9. Epub 2021 Oct 9.

School of Social Development and Public Policy, Fudan University, Shanghai, China.

Background: China committed to establishing a family doctor (FD)-based referral system following the medical reform in 2009. This paper explored the effect of FD on establishing the anticipated system.

Methods: Two waves of survey were conducted in Shanghai, China. 2754 and 1995 individuals were sampled in 2013 and 2016 respectively. We compared orderly visiting behaviour between contracted and non-contracted residents. Logistic regression models were performed to further test the effect of FD on orderly visits.

Results: More contracted residents first-contacted community health service centres (CHSCs; 45.48%) than non-contracted residents (28.93%). Contracted residents were also more likely to refer to specialists via CHSCs than the non-contracted (9.84% vs. 2.60%). The odds ratio (OR) for first-contact at CHSCs by contract status was 1.569 in 2013, but increased to 1.675 in 2016. Being contracted with a FD was associated with referral behaviour, but the OR declined from 2.692 to 2.487 over years.

Conclusion: The survey from Shanghai showed that FD had a significant effect on attracting first-contact at CHSCs and referral via CHSCs; however, the effect on the latter decreased. The effectiveness of the FD role on referral behaviour requires a well-established referral system, which has not yet been completely achieved in China.
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http://dx.doi.org/10.1002/hpm.3346DOI Listing
October 2021

Transmembrane protein 97 exhibits oncogenic properties via enhancing LRP6-mediated Wnt signaling in breast cancer.

Cell Death Dis 2021 Oct 6;12(10):912. Epub 2021 Oct 6.

Guangdong Provincial Key Laboratory of Regional Immunity and Diseases, International Cancer Center, Department of Pharmacology, Shenzhen University Health Science Center, 518055, Shenzhen, China.

Upregulation of transmembrane protein 97 (TMEM97) has been associated with progression and poor outcome in multiple human cancers, including breast cancer. Recent studies suggest that TMEM97 may be involved in the activation of the Wnt/β-catenin pathway. However, the molecular mechanism of TMEM97 action on Wnt/β-catenin signaling is completely unclear. In the current study, TMEM97 was identified as an LRP6-interacting protein. TMEM97 could interact with LRP6 intracellular domain and enhance LRP6-mediated Wnt signaling in a CK1δ/ε-dependent manner. The binding of TMEM97 to LRP6 facilitated the recruitment of CK1δ/ε to LRP6 complex, resulting in LRP6 phosphorylation at Ser 1490 and the stabilization of β-catenin. In breast cancer cells, knockout of TMEM97 attenuated the Wnt/β-catenin signaling cascade via regulating LRP6 phosphorylation, leading to a decrease in the expression of Wnt target genes AXIN2, LEF1, and survivin. TMEM97 deficiency also suppressed cell viability, proliferation, colony formation, migration, invasion, and stemness properties in breast cancer cells. Importantly, TMEM97 knockout suppressed tumor growth through downregulating the Wnt/β-catenin signaling pathway in a breast cancer xenograft model. Taken together, our results revealed that TMEM97 is a positive modulator of canonical Wnt signaling. TMEM97-mediated Wnt signaling is implicated in the tumorigenesis of breast cancer, and its targeted inhibition may be a promising therapeutic strategy for breast cancer.
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http://dx.doi.org/10.1038/s41419-021-04211-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8494741PMC
October 2021

Is a Prognostic Biomarker Correlated With Immune Infiltration in Hepatocellular Carcinoma.

Front Oncol 2021 14;11:713017. Epub 2021 Sep 14.

Department of Pathology, Chongqing Medical University, Chongqing, China.

Background: Recently, it has been reported that angiotensin II receptor-associated protein () plays a substantial role in tumor progression. Nevertheless, the possible role of in hepatocellular carcinoma (HCC) remains unrecognized.

Methods: The metabolic gene rapid visualizer, Cancer Cell Line Encyclopedia, Human Protein Atlas, and Hepatocellular Carcinoma Database were used to analyze the expression of in HCC tissues and normal liver tissues or adjacent tissues. Kaplan-Meier plotter and UALCAN analysis were used to assess the prognostic and diagnostic value of LinkedOmics and cBioPortal were used to explore the genes co-expressed with in HCC. To further understand the potential mechanism of in HCC, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment pathway analyses were performed using software, the protein-protein interaction (PPI) network was established using the STRING database, and the immune infiltration and T-cell exhaustion related to were explored Timer and GEPIA. In addition, immunohistochemistry was used to detect the expression of AGTRAP protein in HCC tissues and paired adjacent tissues from clinical specimens.

Results: This study found that the mRNA and protein levels of AGTRAP in HCC tissues were higher than those in normal liver tissues and adjacent tissues, and higher mRNA levels of were associated with higher histological grade and a poor overall survival in HCC patients. The area under the receiver operating characteristic curve (AUC) of was 0.856, suggesting that it could be a diagnostic marker for HCC. Moreover, the alteration rate of in HCC was 8%, and was involved in HCC probably through the NF-κB and MAPK signaling pathways. Furthermore, was positively correlated with the infiltration of CD8 T cells, CD4 T cells, B cells, macrophages, dendritic cells, and neutrophils, and the levels of were significantly correlated with T-cell exhaustion biomarkers. The immunohistochemistry results confirmed that the protein levels of AGTRAP were consistently higher in HCC tissues than in paired adjacent tissues.

Conclusion: The clinical value of and its correlation with immune infiltration in HCC was effectively identified in clinical data from multiple recognized databases. These findings indicate that could serve as a potential biomarker in the treatment of HCC, thereby informing its prognosis, diagnosis, and even immunotherapy.
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http://dx.doi.org/10.3389/fonc.2021.713017DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8477650PMC
September 2021

Genome-Wide Identification and Functional Characterization of the Trans-Isopentenyl Diphosphate Synthases Gene Family in .

Front Plant Sci 2021 13;12:708697. Epub 2021 Sep 13.

School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China.

Trans-isopentenyl diphosphate synthases (TIDSs) genes are known to be important determinants for terpene diversity and the accumulation of terpenoids. The essential oil of , which is rich in monoterpenes, sesquiterpenes, and other aromatic compounds, has a wide range of pharmacological activities and has therefore attracted considerable interest. However, the gene family, and its relationship to the camphor tree ( L. Presl.), has not yet been characterized. In this study, we identified 10 genes in the genome of the borneol chemotype that were unevenly distributed on chromosomes. Synteny analysis revealed that the gene family in this species likely expanded through segmental duplication events. Furthermore, cis-element analyses demonstrated that TIDS (CcTIDS) genes can respond to multiple abiotic stresses. Finally, functional characterization of eight putative short-chain TIDS proteins revealed that CcTIDS3 and CcTIDS9 exhibit farnesyl diphosphate synthase (FPPS) activity, while CcTIDS1 and CcTIDS2 encode geranylgeranyl diphosphate synthases (GGPPS). Although, CcTIDS8 and CcTIDS10 were found to be catalytically inactive alone, they were able to bind to each other to form a heterodimeric functional geranyl diphosphate synthase (GPPS) , and this interaction was confirmed using a yeast two-hybrid assay. Furthermore, transcriptome analysis revealed that the , , , and genes were found to be more active in roots as compared to stems and leaves, which were verified by quantitative real-time PCR (qRT-PCR). These novel results provide a foundation for further exploration of the role of the gene family in camphor trees, and also provide a potential mechanism by which the production of camphor tree essential oil could be increased for pharmacological purposes through metabolic engineering.
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http://dx.doi.org/10.3389/fpls.2021.708697DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8475955PMC
September 2021

CHD1L prevents lipopolysaccharide-induced hepatocellular carcinomar cell death by activating hnRNP A2/B1-nmMYLK axis.

Cell Death Dis 2021 Sep 29;12(10):891. Epub 2021 Sep 29.

Affiliated Cancer Hospital & Institute, Guangzhou Medical University, Guangzhou, China.

Chromodomain helicase/ATPase DNA-binding protein 1-like gene (CHD1L) has been characterized to be a driver gene in hepatocellular carcinoma (HCC). However, the intrinsic connections between CHD1L and intestinal dysbacteriosis-related inflammation reaction in HCC progression remain incompletely understood. In this study, a specific correlation between CHD1L and nonmuscle isoform of myosin light chain kinase (nmMLCK/nmMYLK), a newly identified molecule associated NF-κB signaling transduction, was disclosed in HCC. CHD1L promotes nmMYLK expression and prevents lipopolysaccharide (LPS) induced tumor cell death. In vitro experiment demonstrated that overexpressed nmMYLK is essential for CHD1L to maintain HCC cell alive, while knocking down nmMYLK significantly attenuate the oncogenic roles of CHD1L. Mechanism analysis revealed that nmMYLK can prevent Caspase-8 from combining with MyD88, an important linker of TLRs signaling pathway, while, knocking down nmMYLK facilitate the MyD88 combines with Caspase-8 and lead to the proteolytic cascade of Caspase as well as the consequent cell apoptosis. Mechanism analysis showed that CHD1L promotes the nmMYLK expression potentially through upregulating the heterogeneous nuclear ribonucleoproteins A2/B1 (hnRNP A2/B1) expression, which can bind to myosin light chain kinase (MYLK) pre-mRNA and lead to the regnant translation of nmMYLK. In summary, this work characterizes a previously unknown role of CHD1L in preventing LPS-induced tumor cell death through activating hnRNP A2/B1-nmMYLK axis. Further inhibition of CHD1L and its downstream signaling could be a novel promising strategy in HCC treatment.
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http://dx.doi.org/10.1038/s41419-021-04167-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8481269PMC
September 2021

anti-PRV activity of dihydromyricetin from .

Nat Prod Res 2021 Sep 29:1-4. Epub 2021 Sep 29.

College of Agriculture, Tongren Polytechnic College, Tongren, China.

Pseudorabies (PR) is an acute infectious disease caused by pseudorabies virus (PRV). There are no available drugs due to the emergence of variant of PRV. Dihydromyricetin (DMY) is a flavonoid extracted from (), which has a variety of pharmacological activities. In this study, we aim to investigate the anti-PRV activity of DMY extracted from . MTT assay was used to detect the cytotoxicity and antiviral activity of DMY. The results detected by flow cytometry and qRT-PCR showed that DMY played anti-PRV role mainly by interfering with the process of virus invasion into host cell and inhibiting the occurrence of pyroptosis This suggested that anti-pyroptosis may be an important antiviral mechanism for DMY which is expected to be a potential anti-PRV drug.
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http://dx.doi.org/10.1080/14786419.2021.1982935DOI Listing
September 2021

Smilax china L. flavonoid alleviates HFHS-induced inflammation by regulating the gut-liver axis in mice.

Phytomedicine 2021 Sep 5:153728. Epub 2021 Sep 5.

Jiangxi Key Laboratory of Natural Product and Functional Food, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China. Electronic address:

Background: Smilax china L., a traditional Chinese herb, has been used to treat various inflammatory disorders, particularly pelvic inflammation. The anti-inflammatory activity of the plant extract has been reported in several in vivo experimental models. However, the underlying anti-inflammatory mechanisms and the role of gut microbiota in mice on Smilax china L. flavonoid (SCF) treatment are poorly understand.

Purpose: To investigate the role of SCF in providing the anti-inflammatory response and the role of gut microbiota in high-fat/high-sucrose (HFHS)-induced obese mice for 12 weeks.

Study Design And Methods: C57BL/6J mice were randomly divided into seven groups, normal chow (NC), HFHS, Orlistat, SCE, and low-, medium-, high- doses of SCF for 12 weeks. The body weight, liver weight, serum concentrations of lipopolysaccharide (LPS), and inflammatory cytokines in mice were assessed. The gene and protein expression levels of inflammation-related markers were measured by qRT-PCR and Western blot. Finally, the composition of gut microbiota was detected by analyzing 16S rDNA gene sequences.

Results: SCF supplement reduced body weight gain, adipose tissue and liver indexes, attenuated serum levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6, LPS, and increased IL-10, and adiponectin. SCF significantly reduced the mRNA expression levels of TNF-α, IL-6, and increased the expression of AMPK, PPAR-γ, and IL-10 in mice's liver and adipose tissues. In addition, the TLR4, p-IκBα, NF-κB, and p65 protein expression levels were reduced after the SCF supplement. Moreover, SCF treatment ameliorated HFHS-induced gut dysbiosis, as revealed by an increased intestinal barrier protective species (Akkermansia spp). The relative abundance of Streptococcaceae, Faecalibaculum, and endotoxin-producing Desulfovibrionaceae were significantly decreased on SCF supplements.

Conclusion: The results showed that SCF effectively inhibits HFHS-induced inflammation by suppressing the LPS-producing bacteria and pro-inflammatory bacteria group. Furthermore, the abundance of gut barrier protective species Akkermansia spp was increased to alleviate inflammatory response, inhibiting the LPS-TLR4/NF-κB signaling pathway. Thus, SCF may be a promising prophylactic for diet-induced inflammatory diseases through the gut-liver axis in mice.
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http://dx.doi.org/10.1016/j.phymed.2021.153728DOI Listing
September 2021

clusterProfiler 4.0: A universal enrichment tool for interpreting omics data.

Innovation (N Y) 2021 Aug 1;2(3):100141. Epub 2021 Jul 1.

Department of Bioinformatics, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.

Functional enrichment analysis is pivotal for interpreting high-throughput omics data in life science. It is crucial for this type of tool to use the latest annotation databases for as many organisms as possible. To meet these requirements, we present here an updated version of our popular Bioconductor package, clusterProfiler 4.0. This package has been enhanced considerably compared with its original version published 9 years ago. The new version provides a universal interface for functional enrichment analysis in thousands of organisms based on internally supported ontologies and pathways as well as annotation data provided by users or derived from online databases. It also extends the and packages to offer tidy interfaces for data operation and visualization. Other new features include gene set enrichment analysis and comparison of enrichment results from multiple gene lists. We anticipate that clusterProfiler 4.0 will be applied to a wide range of scenarios across diverse organisms.
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http://dx.doi.org/10.1016/j.xinn.2021.100141DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8454663PMC
August 2021

Comparative genomics provides insights into the aquatic adaptations of mammals.

Proc Natl Acad Sci U S A 2021 Sep;118(37)

BGI-Qingdao, BGI-Shenzhen, Qingdao 266555, China.

The ancestors of marine mammals once roamed the land and independently committed to an aquatic lifestyle. These macroevolutionary transitions have intrigued scientists for centuries. Here, we generated high-quality genome assemblies of 17 marine mammals (11 cetaceans and six pinnipeds), including eight assemblies at the chromosome level. Incorporating previously published data, we reconstructed the marine mammal phylogeny and population histories and identified numerous idiosyncratic and convergent genomic variations that possibly contributed to the transition from land to water in marine mammal lineages. Genes associated with the formation of blubber (), vascular development (), and heat production by brown adipose tissue () had unique changes that may contribute to marine mammal thermoregulation. We also observed many lineage-specific changes in the marine mammals, including genes associated with deep diving and navigation. Our study advances understanding of the timing, pattern, and molecular changes associated with the evolution of mammalian lineages adapting to aquatic life.
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http://dx.doi.org/10.1073/pnas.2106080118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8449357PMC
September 2021

Evaluation of trans fatty acids, carbonyl compounds and bioactive minor components in commercial linseed oils.

Food Chem 2022 Feb 22;369:130930. Epub 2021 Aug 22.

Department of Entomology and Nematology, University of California, Davis, CA 95616, USA. Electronic address:

Trans fatty acids (TFAs), associated with the risks of coronary heart disease and diabetes, are formed by isomerization of unsaturated fatty acids during refining of linseed oils. In this study, TFAs and the chemical characteristics (acid value, peroxide value, carbonyl compounds, bioactive minor components and fatty acids) in 32 commercial linseed oils were investigated, and the correlation among them were further analyzed. Results showed that C18:3 TFAs were predominant TFAs in linseed oils and about 9% of the samples had TFA contents above 2 g/100 g fat, as well as the average level of TFA in the refined samples was higher than that in the unrefined oils. The correlation analyses suggested C18:3 TFAs exhibited significant negative correlations with acid value, levels of acetone, trans-2-nonenal, campesterol and α-linolenic acid. These results provided a comprehensive insight of TFAs in linseed oil and had important implications for consumers and linseed oil industry.
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http://dx.doi.org/10.1016/j.foodchem.2021.130930DOI Listing
February 2022

Acetylcholine-synthesizing macrophages in subcutaneous fat are regulated by β -adrenergic signaling.

EMBO J 2021 Aug 30:e106061. Epub 2021 Aug 30.

Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA.

Non-neuronal cholinergic signaling, mediated by acetylcholine, plays important roles in physiological processes including inflammation and immunity. Our group first discovered evidence of non-neuronal cholinergic circuitry in adipose tissue, whereby immune cells secrete acetylcholine to activate beige adipocytes during adaptive thermogenesis. Here, we reveal that macrophages are the cellular protagonists responsible for secreting acetylcholine to regulate thermogenic activation in subcutaneous fat, and we term these cells cholinergic adipose macrophages (ChAMs). An adaptive increase in ChAM abundance is evident following acute cold exposure, and macrophage-specific deletion of choline acetyltransferase (ChAT), the enzyme for acetylcholine biosynthesis, impairs the cold-induced thermogenic capacity of mice. Further, using pharmacological and genetic approaches, we show that ChAMs are regulated via adrenergic signaling, specifically through the β adrenergic receptor. These findings demonstrate that macrophages are an essential adipose tissue source of acetylcholine for the regulation of adaptive thermogenesis, and may be useful for therapeutic targeting in metabolic diseases.
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http://dx.doi.org/10.15252/embj.2020106061DOI Listing
August 2021

Cytokine storms and pyroptosis are primarily responsible for the rapid death of mice infected with pseudorabies virus.

R Soc Open Sci 2021 Aug 25;8(8):210296. Epub 2021 Aug 25.

College of Agriculture, Tongren Polytechnic College, Bijiang District, Tongren City, Guizhou 554300, People's Republic of China.

Pseudorabies virus (PRV), the causative agent of Aujeszky's disease, is one of the most harmful pathogens to the pig industry. PRV can infect and kill a variety of mammals. Nevertheless, the underlying pathogenesis related to PRV is still unclear. This study aims to investigate the pathogenesis induced by PRV in a mouse model. The mice infected with the PRV-HLJ strain developed severe clinical manifestations at 36 h post-infection (hpi), and mortality occurred within 48-72 hpi. Hematoxylin-eosin staining and qRT-PCR methods were used to detect the pathological damage and expression of cytokines related to an immune reaction in brain tissue, respectively. The cytokine storms caused by IFN-α, IFN-, TNF-α, IL-1, IL-6 and IL-18 were related to the histopathological changes induced by PRV. This pattern of cytokine secretion depicts an image of typical cytokine storms, characterized by dysregulated secretion of pro-inflammatory cytokines and imbalanced pro-inflammatory and anti-inflammatory responses. In addition, the pyroptosis pathway was also activated by PRV by elevating the expression levels of nod-like receptor protein 3, Caspase-1, Gasdermin-D and interleukin-1/18. These findings provide a way for further understanding the molecular basis in PRV pathogenesis.
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http://dx.doi.org/10.1098/rsos.210296DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8385338PMC
August 2021

Robust Multi-Frame Super-Resolution Based on Adaptive Half-Quadratic Function and Local Structure Tensor Weighted BTV.

Sensors (Basel) 2021 Aug 17;21(16). Epub 2021 Aug 17.

College of Computer Science, Sichuan University, Chengdu 610065, China.

It is difficult to improve image resolution in hardware due to the limitations of technology and too high costs, but most application fields need high resolution images, so super-resolution technology has been produced. This paper mainly uses information redundancy to realize multi-frame super-resolution. In recent years, many researchers have proposed a variety of multi-frame super-resolution methods, but it is very difficult to preserve the image edge and texture details and remove the influence of noise effectively in practical applications. In this paper, a minimum variance method is proposed to select the low resolution images with appropriate quality quickly for super-resolution. The half-quadratic function is used as the loss function to minimize the observation error between the estimated high resolution image and low-resolution images. The function parameter is determined adaptively according to observation errors of each low-resolution image. The combination of a local structure tensor and Bilateral Total Variation (BTV) as image prior knowledge preserves the details of the image and suppresses the noise simultaneously. The experimental results on synthetic data and real data show that our proposed method can better preserve the details of the image than the existing methods.
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http://dx.doi.org/10.3390/s21165533DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402259PMC
August 2021

Sentinel lymph node mapping in early-stage cervical cancer: Meta-analysis.

Medicine (Baltimore) 2021 Aug;100(34):e27035

Department of Obstetrics and Gynecology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, P.R. China.

Background: The value of sentinel lymph node (SLN) mapping for early-stage cervical cancer remains controversial. Therefore, we collected data to investigate the feasibility and diagnostic accuracy of SLN in patients with early-stage (IA-IIA) cervical cancer.

Methods: We searched Embase, PubMed, and the Cochrane Library databases issued before June 1, 2020. The sample size of the selected study was at least 10 patients with early-stage (IA-IIA) cervical cancer, the pooled detection rates and the separate detection rate (overall detection rate, bilateral detection rate) using blue dye with Tc, technetium 99 (Tc) and indocyanine green (ICG) technique of early-stage cervical cancer was reported. R-3.6.1 software was used to evaluate pooled detection rate and sensitivity.

Results: Two thousand one hundred sixty-four patients included for analysis in 28 studies ranging from 12 to 405 patients. The combined overall detection rate of SLN mapping was 95% with a 72% pooled bilateral detection rate. The sensitivity of the combined overall detection rate of SLN mapping was 94.99% as well as a sensitivity of 72.43% bilateral detection rate. The overall detection rate of SLN was 96% for blue dye with Tc, 95% for Tc, 98% for ICG technique. The bilateral detection rate of SLN was 76% for blue dye with Tc, 63% for Tc, 85% for ICG technique. The sensitivity of the overall detection rate of SLN mapping was 97.76% as well as a sensitivity of 84.96% bilateral detection rate of ICG technique.

Conclusion: In early-stage cervical cancer, overall detection rate of SLN mapping is elevated while bilateral detection rate is lower. The overall detection rate (98%) as well as bilateral rate (85%) of ICG seems to be a better SLN mapping technique among the method of SLN mapping (using blue dye with Tc, Tc or ICG). We believe SLN mapping may be considered contemporary technique which could provide additional benefits over traditional pelvic lymphadenectomy. While promising results in SLN mapping has been found, larger patient samples, including randomized studies, are required at the same time.
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http://dx.doi.org/10.1097/MD.0000000000027035DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8389869PMC
August 2021

Eldecalcitol inhibits the progression of oral cancer by suppressing the expression of GPx-1.

Oral Dis 2021 Aug 24. Epub 2021 Aug 24.

Center of Osteoporosis and Bone Mineral Research, Shandong University, Jinan, China.

Objectives: This study aimed to investigate the role of eldecalcitol in the progression of oral squamous cell carcinoma and to explore the related mechanism.

Materials And Methods: The effects of eldecalcitol on the proliferation, cell cycle, apoptosis, and migration of oral cancer cells (SCC-15 and CAL-27) were evaluated with cell counting kit-8, flow cytometry, quantitative real-time polymerase chain reaction, western blotting, and scratch assay. Mouse xenograft tumor model was established to further confirm the role of eldecalcitol in the progression of oral cancer. Immunohistochemistry, quantitative real-time polymerase chain reaction, and western blotting were used to detect glutathione peroxidase-1 expression in oral cancer tissue and cells treated with eldecalcitol.

Results: Eldecalcitol was found to inhibit the proliferation and migration of SCC-15 and CAL-27 cells significantly, block the cell cycle in the G0/G1 phase, and enhance the apoptosis. In addition, glutathione peroxidase-1 was downregulated by eldecalcitol and acted as an important medium of eldecalcitol in inhibiting the proliferation and migration of SCC-15 and CAL-27 cells, as well as promoting their apoptosis.

Conclusions: Eldecalcitol may inhibit the progression of oral cancer by suppressing the expression of glutathione peroxidase-1, which may provide new insight into the application of eldecalcitol as a potential anti-cancer drug.
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http://dx.doi.org/10.1111/odi.14010DOI Listing
August 2021

Aggregation-Induced Emission Materials that Aid in Pharmaceutical Research.

Adv Healthc Mater 2021 Aug 21:e2101067. Epub 2021 Aug 21.

State Key Laboratory of Luminescent Materials and Devices, Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregates, Center for Aggregation-Induced Emission, South China University of Technology, Guangzhou, 510640, China.

The in situ visualization of drugs can improve the understanding of their pharmacokinetics and mechanisms. Aggregation-induced emission (AIE) materials, which can aid in the visualization of drugs, are gradually being employed in pharmaceutical research due to their excellent fluorescence properties, good biocompatibility, and extremely high sensitivity. Herein, the progress of AIE materials in pharmaceutical research, including AIE carriers for drug delivery, AIE multifunctional prodrugs, and AIE compounds as bioactive reagents for theranostics is briefly described. Moreover, the opportunities and challenges of AIE materials in pharmaceutical research are discussed in depth. It is believed that versatile AIE materials hold great promise for the promotion of pharmacological research and can facilitate significant advancements in clinical fields.
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http://dx.doi.org/10.1002/adhm.202101067DOI Listing
August 2021

Chromatin lncRNA Platr10 controls stem cell pluripotency by coordinating an intrachromosomal regulatory network.

Genome Biol 2021 08 19;22(1):233. Epub 2021 Aug 19.

Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong, 510530, People's Republic of China.

Background: A specific 3-dimensional intrachromosomal architecture of core stem cell factor genes is required to reprogram a somatic cell into pluripotency. As little is known about the epigenetic readers that orchestrate this architectural remodeling, we used a novel chromatin RNA in situ reverse transcription sequencing (CRIST-seq) approach to profile long noncoding RNAs (lncRNAs) in the Oct4 promoter.

Results: We identify Platr10 as an Oct4 - Sox2 binding lncRNA that is activated in somatic cell reprogramming. Platr10 is essential for the maintenance of pluripotency, and lack of this lncRNA causes stem cells to exit from pluripotency. In fibroblasts, ectopically expressed Platr10 functions in trans to activate core stem cell factor genes and enhance pluripotent reprogramming. Using RNA reverse transcription-associated trap sequencing (RAT-seq), we show that Platr10 interacts with multiple pluripotency-associated genes, including Oct4, Sox2, Klf4, and c-Myc, which have been extensively used to reprogram somatic cells. Mechanistically, we demonstrate that Platr10 helps orchestrate intrachromosomal promoter-enhancer looping and recruits TET1, the enzyme that actively induces DNA demethylation for the initiation of pluripotency. We further show that Platr10 contains an Oct4 binding element that interacts with the Oct4 promoter and a TET1-binding element that recruits TET1. Mutation of either of these two elements abolishes Platr10 activity.

Conclusion: These data suggest that Platr10 functions as a novel chromatin RNA molecule to control pluripotency in trans by modulating chromatin architecture and regulating DNA methylation in the core stem cell factor network.
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http://dx.doi.org/10.1186/s13059-021-02444-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8375132PMC
August 2021

An efficient chromium(III)-catalyzed aerobic oxidation of methylarenes in water for the green preparation of corresponding acids.

Dalton Trans 2021 Sep 21;50(36):12413-12418. Epub 2021 Sep 21.

Key Lab of Organic Optoelectronics & Molecular Engineering of Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, P.R. China.

A highly efficient method to oxidize methylarenes to their corresponding acids with a reusable Cr catalyst was developed. The reaction can be carried out in water with 1 atm oxygen and KSO as cooxidants, proceeds under green and mild conditions, and is suitable for the oxidation of both electron-deficient and electron-rich methylarenes, including heteroaryl methylarenes, even at the gram level. The excellent result, together with its simplicity of operation and the ability to continuously reuse the catalyst, makes this new methodology environmentally benign and cost-effective. The generality of this methodology gives it the potential for use on an industrial scale. Differing from the accepted oxidation mechanism of toluene, GC-MS studies and DFT calculations have revealed that the key benzyl alcohol intermediate is formed under the synergetic effect of the chromium and molybdenum in the Cr catalyst, which can be further oxidized to afford benzaldehyde and finally benzoic acid.
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http://dx.doi.org/10.1039/d1dt01967hDOI Listing
September 2021

Notum suppresses the osteogenic differentiation of periodontal ligament stem cells through the Wnt/Beta catenin signaling pathway.

Arch Oral Biol 2021 Oct 21;130:105211. Epub 2021 Jul 21.

Department of Bone Metabolism, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, 250012, China. Electronic address:

Objectives: The aims of this study were to explore: (ⅰ) the effect of Notum on periodontitis in vivo; (ⅱ) the effect of Notum on the osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) in vitro; and (ⅲ) the potential mechanism of Notum in inhibiting the osteogenic differentiation of hPDLSCs.

Design: C57BL/6J mice were randomly assigned into two groups: control group (n = 4) and periodontitis group (n = 4). Immunohistochemical staining was used to evaluate the expression of Notum. In in vitro experiments, Western blot, qRT- PCR and ELISA were used to examine the expression of Notum in a lipopolysaccharide-induced inflammation model. Alkaline phosphatase staining was used to evaluate alkaline phosphatase activity. Western blot and qRT - PCR were used to measure the expression of osteogenic-related markers after adding human recombinant Notum and Notum inhibitor ABC99. In addition, LiCl, an agonist of the Wnt/Beta-catenin signaling pathway, was added to explore using Western blot whether Notum was involved in regulating the osteogenic differentiation of human periodontal ligament stem cells through the Wnt/Beta-catenin signaling pathway.

Results: Notum was highly expressed in periodontal tissues of mice and lipopolysaccharide-induced inflammation cell model. The protein and messenger ribonucleic acid levels of hPDLSCs osteogenic markers were reduced after adding human recombinant Notum. However, the inhibitory effect of Notum on the osteogenic differentiation of hPDLSCs could be significantly reversed by adding LiCl.

Conclusion: These results demonstrated that Notum inhibited the osteogenic differentiation of hPDLSCs probably via the Wnt/Beta-catenin the downstream signaling pathway.
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http://dx.doi.org/10.1016/j.archoralbio.2021.105211DOI Listing
October 2021

Low-Density Fluorinated Silane Solvent Enhancing Deep Cycle Lithium-Sulfur Batteries' Lifetime.

Adv Mater 2021 Sep 2;33(38):e2102034. Epub 2021 Aug 2.

Beijing Advanced Innovation Center for Materials Genome Engineering, Key Laboratory for Renewable Energy, Beijing Key Laboratory for New Energy Materials and Devices, Institute of Physics, Chinese Academy of Sciences, Beijing, 100190, China.

The lithium metal anode (LMA) instability at deep cycle with high utilization is a crucial barrier for developing lithium (Li) metal batteries, resulting in excessive Li inventory and electrolyte demand. This issue becomes more severe in capacity-type lithium-sulfur (Li-S) batteries. High-concentration or localized high-concentration electrolytes are noted as effective strategies to stabilize Li metal but usually lead to a high electrolyte density (>1.4 g mL ). Here we propose a bifunctional fluorinated silane-based electrolyte with a low density of 1.0 g mL that not only is much lighter than conventional electrolytes (≈1.2 g mL ) but also form a robust solid electrolyte interface to minimize Li depletion. Therefore, the Li loss rate is reduced over 4.5-fold with the proposed electrolyte relative to its conventional counterpart. When paired with onefold excess LMA at the electrolyte weight/cell capacity (E/C) ratio of 4.5 g Ah , the Li-S pouch cell using our electrolyte can survive for 103 cycles, much longer than with the conventional electrolyte (38 cycles). This demonstrates that our electrolyte not only reduces the E/C ratio but also enhances the cyclic stability of Li-S batteries under limited Li amounts.
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http://dx.doi.org/10.1002/adma.202102034DOI Listing
September 2021

The BTB/POZ domain protein GmBTB/POZ promotes the ubiquitination and degradation of the soybean AP2/ERF-like transcription factor GmAP2 to regulate the defense response to Phytophthora sojae.

J Exp Bot 2021 Aug 2. Epub 2021 Aug 2.

Soybean Research Institute of Northeast Agricultural University, Key Laboratory of Soybean Biology of Chinese Education Ministry, Harbin, China.

Phytophthora root and stem rot in soybean [Glycine max (L.) Merr.] is a destructive disease worldwide. Improving soybean resistance to the causal pathogen, Phytophthora sojae, is a major target for breeders; however, it remains largely unclear how the pathogen regulates the various affected signaling pathways in the host, which consist of complex networks including key transcription factors and their targets. We previously demonstrated that GmBTB/POZ enhances soybean resistance to P. sojae and associated defense response. Here, we report that GmBTB/POZ interacts with transcription factor GmAP2 and promotes the ubiquitination of GmAP2. The GmAP2-RNAi transgenic soybean hairy roots exhibited an enhanced resistance to P. sojae, whereas GmAP2-overexpressing hairy roots showed P. sojae hypersensitivity. Subsequently, GmWRKY33 was identified as a target of GmAP2, which represses its expression by directly binding to the GmWRKY33 promoter. GmWRKY33 acts as a positive regulator in the response of soybean to P. sojae. Additionally, the overexpression of GmBTB/POZ released the GmAP2-regulated suppression of GmWRKY33 expression in the GmAP2-OE soybean hairy roots and increased their resistance to P. sojae. Taken together, these results indicate a novel regulatory mechanism, the GmBTB/POZ-GmAP2 modulation of the P. sojae resistance response, which putatively regulates the downstream target gene GmWRKY33 in soybean.
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http://dx.doi.org/10.1093/jxb/erab363DOI Listing
August 2021

Prognostic marker identification based on weighted gene co-expression network analysis and associated confirmation in gastric cancer.

Bioengineered 2021 12;12(1):4666-4680

Department of Clinical Laboratory, The Affiliated Hospital of Guilin Medical University, Guangxi, Guilin, China.

The aim of this study was to explore the potential molecular mechanisms of Gastric cancer (GC) and identify new prognostic markers for GC. RNA sequencing data were downloaded from the Gene Expression Omnibus database, and 418 differentially expressed genes (DEGs) were screened. Weighted correlation network analysis (WGCNA) was performed to identify six hub modules related to the clinical features of GC. Cytoscape software was used to identify five hub genes in the co-expression network, including CST1, CEMIP, COL8A1, PMEPA1, and MSLN. The TCGA database was used to verify hub gene expression in GC. The overall survival in the high CEMIP expression group was significantly lower than that of patients in the low CEMIP expression group. CEMIP expression was also found to be negatively correlated with B cell and CD4 + T cell infiltration. Further, associated in vitro experiments confirmed that CEMIP downregulation suppressed the proliferation and migration of GC cells and impaired the chemoresistance of GC cells to 5-fluorouracil.Our study effectively identified and validated prognostic biomarkers for GC, laying a new foundation for the therapeutic target, occurrence, and development of gastric cancer.
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http://dx.doi.org/10.1080/21655979.2021.1957645DOI Listing
December 2021

Highly sensitive time-resolved fluoroimmunoassay for the quantitative onsite detection of Alternaria longipes in tobacco.

J Appl Microbiol 2021 Jul 27. Epub 2021 Jul 27.

China National Tobacco Quality Supervision and Test Center, Zhengzhou, China.

Aims: Alternaria longipes is a causal agent of brown spot of tobacco, which remains a serious threat to tobacco production. Herein, we established a detection method for A. longipes in tobacco samples based on the principle of time-resolved fluoroimmunoassay, in order to fulfil the requirement of rapid, sensitive and accurate detection in situ.

Methods And Results: A monoclonal antibody against A. longipes was generated, and its purity and titration were assessed using western blot and ELISA. The size of europium (III) nanospheres was measured to confirm successful antibody conjugation. The method described here can detect A. longipes protein lysates as low as 0.78 ng ml , with recovery rates ranging from 85.96% to 99.67% in spiked tobacco. The specificity was also confirmed using a panel of microorganisms.

Conclusions: The fluorescent strips allow rapid and sensitive onsite detection of A. longipes in tobacco samples, with high accuracy, specificity, and repeatability.

Significance And Impact Of The Study: This novel detection method provides convenience of using crude samples without complex procedures, and therefore allows rapid onsite detection by end users and quick responses towards A. longipes, which is critical for disease control and elimination of phytopathogens.
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http://dx.doi.org/10.1111/jam.15233DOI Listing
July 2021

[Retracted] MicroRNA‑148a inhibits migration and invasion of ovarian cancer cells via targeting sphingosine‑1‑phosphate receptor 1.

Mol Med Rep 2021 Sep 19;24(3). Epub 2021 Jul 19.

Department of Gynecology and Obstetrics, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China.

Following the publication of this paper, it was drawn to the Editors' attention by a concerned reader that certain of the western blotting data shown in Fig. 4A and B were strikingly similar to data appearing in different form in other articles by different authors. Owing to the fact that the contentious data in the above article had already been published elsewhere, or were already under consideration for publication, prior to its submission to , the Editor has decided that this paper should be retracted from the Journal. The authors were asked for an explanation to account for these concerns, but the Editorial Office did not receive any reply. The Editor apologizes to the readership for any inconvenience caused. [the original article was published in 12: 3775‑3780, 2015; DOI: 10.3892/mmr.2015.3827].
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http://dx.doi.org/10.3892/mmr.2021.12298DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8299192PMC
September 2021

[Retracted] MicroRNA‑148a inhibits the proliferation and promotes the paclitaxel‑induced apoptosis of ovarian cancer cells by targeting PDIA3.

Mol Med Rep 2021 Sep 19;24(3). Epub 2021 Jul 19.

Department of Gynecology and Obstetrics, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China.

Following the publication of this paper, it was drawn to the Editors' attention by a concerned reader that the cell apoptosis assay data shown in Figs. 1C and 4D were strikingly similar to data appearing in different form in other articles by different authors. Owing to the fact that the contentious data in the above article had already been published elsewhere, or were already under consideration for publication, prior to its submission to , the Editor has decided that this paper should be retracted from the Journal. The authors were asked for an explanation to account for these concerns, but the Editorial Office did not receive any reply. The Editor apologizes to the readership for any inconvenience caused. [the original article was published in 12: 3923‑3929, 2015; DOI: 10.3892/mmr.2015.3826].
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http://dx.doi.org/10.3892/mmr.2021.12294DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8299188PMC
September 2021

Glycerol-3-phosphate biosynthesis regenerates cytosolic NAD to alleviate mitochondrial disease.

Cell Metab 2021 Oct 15;33(10):1974-1987.e9. Epub 2021 Jul 15.

Graduate School of Peking Union Medical College, Beijing 100730, China; National Institute of Biological Sciences, Beijing 102206, China; Beijing Key Laboratory of Cell Biology for Animal Aging, Beijing 102206, China; Tsinghua Institute of Multidisciplinary Biomedical Research, Tsinghua University, Beijing 102206, China. Electronic address:

Electron transport chain (ETC) dysfunction or hypoxia causes toxic NADH accumulation. How cells regenerate NAD under such conditions remains elusive. Here, integrating bioinformatic analysis and experimental validation, we identify glycerol-3-phosphate (Gro3P) biosynthesis as an endogenous NAD-regeneration pathway. Under genetic or pharmacological ETC inhibition, disrupting Gro3P synthesis inhibits yeast proliferation, shortens lifespan of C. elegans, impairs growth of cancer cells in culture and in xenografts, and causes metabolic derangements in mouse liver. Moreover, the Gro3P shuttle selectively regenerates cytosolic NAD under mitochondrial complex I inhibition; enhancing Gro3P synthesis promotes shuttle activity to restore proliferation of complex I-impaired cells. Mouse brain has much lower levels of Gro3P synthesis enzymes as compared with other organs. Strikingly, enhancing Gro3P synthesis suppresses neuroinflammation and extends lifespan in the Ndufs4 mice. Collectively, our results reveal Gro3P biosynthesis as an evolutionarily conserved coordinator of NADH/NAD redox homeostasis and present a therapeutic target for mitochondrial complex I diseases.
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http://dx.doi.org/10.1016/j.cmet.2021.06.013DOI Listing
October 2021
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