Publications by authors named "Shanshan Li"

1,073 Publications

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Prenatal exercise reprograms the development of hypertension progress and improves vascular health in SHR offspring.

Vascul Pharmacol 2021 Jun 8:106885. Epub 2021 Jun 8.

Department of Exercise Physiology, Beijing Sport University, Beijing 100084, China; Key Laboratory of Physical Fitness and Exercise, Ministry of Education, Beijing Sport University, Beijing, China. Electronic address:

Background: Upregulation of L-type voltage-gated Ca (Ca1.2) channel in the arterial myocytes is a hallmark feature of hypertension. However, whether maternal exercise during pregnancy has a sustained beneficial effect on the offspring of spontaneously hypertensive rats (SHRs) through epigenetic regulation of Ca1.2 channel is largely unknown.

Methods: Pregnant SHRs and Wistar-Kyoto rats were subjected to swimming and the vascular molecular and functional properties of male offspring were evaluated at embryonic (E) 20.5 day, 3 months (3 M), and 6 months (6 M).

Results: Exercise during pregnancy significantly decreased the resting blood pressure at 3 M but not 6 M in the offspring of SHR. Prenatal exercise significantly reduced the cardiovascular reactivity, the contribution of Ca1.2 channel to the vascular tone, and the whole-cell current density of Ca1.2 channel in both 3 M and 6 M offspring of SHR. Moreover, maternal exercise triggered hypermethylation of the promoter region of the Ca1.2 α1C gene (CACNA1C), with a concomitant decrease in its protein and mRNA expressions in SHR offspring at E20.5, 3 M, and 6 M. Tissue culture experiments further confirmed that 5-Aza-2'-deoxycytidine increased the structure and functional expression of Ca1.2 channel by inhibiting the DNA methylation of CACNA1C. However, the improvement of prenatal exercise on the blood pressure, function, and expression of Ca1.2 channel was attenuated in the offspring of SHRs at 6 M compared to the 3 M readout.

Conclusions: These data suggest that prenatal exercise improves the vascular function by the hypermethylation of CACNA1C in the arterial myocytes and delays the development of hypertension in the offspring of SHRs. However, these effects fade out with age.
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http://dx.doi.org/10.1016/j.vph.2021.106885DOI Listing
June 2021

Modeling leukemia with pediatric acute leukemia patient-derived iPSCs.

Stem Cell Res 2021 May 25;54:102404. Epub 2021 May 25.

Department of Hematology & Oncology, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University, National Health Committee Key Laboratory of Pediatric Hematology & Oncology, Shanghai 200127, China. Electronic address:

Objective: ediatric acute leukemia (AL) is the most common hematological malignancy in childhood. However, the limitation of clinical specimens hindered the progress of research. Therefore, new research platforms are urgently needed to establish and clarify the pathogenesis of pediatric AL, and it is necessary to try to find novel targeted therapies for the clinical use. Here, the induced pluripotent stem cells (iPSCs) derived from AL provide a reliable model for basic research.

Methods: eukemia cells were sorted by flow cytometry and then reprogrammed into iPSCs by Sendai virus. Cell cycle assay was used to analyze cell proliferation.

Results: iPS cell lines from T cell acute lymphoblastic leukemia (T-ALL) and acute myeloid leukemia (AML) cells were successfully established. The reprogramming efficiency of AML cells was much higher than that of ALL cells. Disease iPS cells switched off the expression of the disease marker genes at iPS and HPC stage. When different subtypes of AML-iPSCs were differentiated into hematopoietic progenitor cells, iPS derived from acute megakaryocytic leukemia was more readily differentiated into megakaryocyte-erythroid progenitors. Whereas, the differentiation of multipotent lymphoid progenitor (MLP) and granulocyte macrophage progenitor (GMP) were blocked. The iPS derived from acute monocyte leukemia (AMCL) also showed the differentiation of common myeloid progenitors (CMP), GMP and monocytes significantly increased but MLP differentiation was inhibited. The AML-iPSC could form teratomas and we could obverse three germ layers in vivo, indicating that the AML-iPSCs have full pluripotency. However, there were not enough blood cells in teratoma to identify the leukemia.

Conclusions: Our results provide a novel platform for AL research and critical insight into the difference of hematopoietic differentiation between ALL and AML.
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http://dx.doi.org/10.1016/j.scr.2021.102404DOI Listing
May 2021

Effect of exercise intervention on primary insomnia: a meta-analysis.

J Sports Med Phys Fitness 2021 Jun;61(6):857-866

Institute of Sport Science, Sichuan University, Chengdu, China -

Introduction: Exercise can have a positive effect on body functioning and immunity. This study systematically evaluated the effect of exercise intervention on patients diagnosed with primary insomnia. Based on the meta-analysis, we developed exercise recommendations to help improving sleep quality in these patients.

Evidence Acquisition: Using a combination of manual and electronic retrieval strategies, we examined the CNKI, VIP, Wanfang, Web of Science, SpringerLink, EBSCO, PubMed, Cochrane Library, and Embase databases; and identified randomized controlled trials that addressed the effects of exercise intervention on primary insomnia and were published before October 2019. Risk of bias was assessed based on the Cochrane Handbook 5.1.0, and meta-analysis was conducted using STATA 13.0 (StataCorp LLC; College Station, TX, USA).

Evidence Synthesis: Data were included for 1269 patients who received exercise interventions and 1203 patients who received drug therapy or no intervention (controls) in 23 trials of high quality. Meta-analysis showed that exercise intervention had a significant effect on the treatment of primary insomnia (SMD: -1.64, 95% CI: -2.08 to -1.19, P<0.001). Subgroup analysis showed significant effect sizes for older patients (>60 years) (SMD: -1.69, 95% CI: -2.40 to -0.97, P<0.001), aerobic exercise (SMD: -2.21, 95% CI: -2.89 to -1.53, P<0.001), interventions lasting 8-12 weeks (SMD: -2.58, 95% CI: -3.61 to -1.54, P<0.001), interventions lasting ≤60 min (SMD: -2.29, 95% CI: -3.66 to -0.92, P=0.001), Asian patients (SMD: -1.86, 95% CI: -2.42 to -1.31, P<0.001), and interventions ≤4 times/week (SMD: -1.70, 95% CI: -2.29 to -1.11, P<0.001). Both bias and sensitivity analyses suggested that our meta-analysis gave robust results.

Conclusions: Our results indicate that exercise intervention has a significant positive influence on primary insomnia, especially for older patients. We recommend that an exercise regimen of 60 min 4-5 times a week for 8-12 weeks can improve sleep quality and treat the symptoms of primary insomnia. Future studies should focus on verifying and extending our results by increasing sample size and improving the quality of studies included.
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http://dx.doi.org/10.23736/S0022-4707.21.11443-4DOI Listing
June 2021

Polyketide pesticides from actinomycetes.

Curr Opin Biotechnol 2021 Jun 5;69:299-307. Epub 2021 Jun 5.

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China. Electronic address:

Natural product derived pesticides have increased in popularity worldwide because of their high efficacy, eco-friendly nature and favorable safety profile. The development of polyketide pesticides from actinomycetes reflects this increase in popularity in the past decades. These pesticides, which include avermectins, spinosyns, polynactins, tetramycin and their analogues, have been successfully applied in crop protection. Moreover, the advance of biotechnology has led to continuous improvement in the discovery and production processes. In this review, we summarize these polyketide pesticides, their activities and provide insight into their development. We also discuss engineering strategies and the current status of industrial production for these pesticides. Given that actinomycetes are known to produce a wide range of bioactive secondary metabolites, the description of pesticide development and high yield strain improvement presented herein will facilitate further development of these valuable polyketide pesticides from actinomycetes.
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http://dx.doi.org/10.1016/j.copbio.2021.05.006DOI Listing
June 2021

Fe(III) reduction due to low pe+pH contributes to reducing Cd transfer within a soil-rice system.

J Hazard Mater 2021 08 15;415:125668. Epub 2021 Mar 15.

Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture and Rural Affairs/ Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China. Electronic address:

Effect of Fe redox state caused by low soil pe+pH levels on Cd uptake by rice is unclear. Rice grown in pots of Cd-contaminated paddy soil were subjected to different irrigation regimes: flooding, intermittent flooding (Int-FL), and sustained soil moisture at 70% water holding capacity (WHC). Results showed low pe+pH (5.52 and 7.09) in flooding treatment significantly increased relative abundances of Fe-reducing bacteria (FeRB) (6.29% and 4.51%), especially members within the Clostridium, Geobacter and Desulfuromonadia genera. Stimulation of FeRB activity induced Fe(III) reduction and increased Fe content in flooded soils, which promoted Cd sequestration in low-crystalline fraction of IP (IP-Feh-Cd) and Cd bonded to amorphous Fe-oxides (amFeox-Cd). The 24.9-62.4% higher amFeox-Cd content was the important factor for 20.4-44.2% lower CaCl-extractable Cd content in flooding treatment than those in other treatments. Soil submergence reduced Cd uptake by rice at tillering and booting stages, the critical periods of Cd transport in the soil-rice system, which was attributed to the increases in dissolved Fe and IP-Feh-Cd contents and decrease in CaCl-Cd content. Therefore, maintaining flooding during the tillering and booting stages may be an effective strategy to reduce Cd uptake by rice cultivated in Cd-contaminated soil.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125668DOI Listing
August 2021

The impacts of long-term exposure to PM on cancer hospitalizations in Brazil.

Environ Int 2021 Jun 1;154:106671. Epub 2021 Jun 1.

School of Public Health and Preventive Medicine, Monash University, Melbourne, Australia. Electronic address:

Background: Long-term exposure to PM has been linked to cancer incidence and mortality. However, it was unknown whether there was an association with cancer hospitalizations.

Methods: Data on cancer hospitalizations and annual PM concentrations were collected from 1,814 Brazilian cities during 2002-2015. A difference-in-difference approach with quasi-Poisson regression was applied to examine State-specific associations. The State-specific associations were pooled at a national level using random-effect meta-analyses. PM attributable burden were estimated for cancer hospitalization admissions, inpatient days and costs.

Results: We included 5,102,358 cancer hospitalizations (53.8% female). The mean annual concentration of PM was 7.0 μg/m (standard deviation: 4.0 μg/m). With each 1 μg/m increase in two-year-average (current year and previous one year) concentrations of PM, the relative risks (RR) of hospitalization were 1.04 (95% confidence interval [CI]: 1.02 to 1.07) for all-site cancers from 2002 to 2015 without sex and age differences. We estimated that 33.82% (95%CI: 14.97% to 47.84%) of total cancer hospitalizations could be attributed to PM exposure in Brazil during the study time. For every 100,000 population, 1,190 (95%CI: 527 to 1,836) cancer hospitalizations, 8,191 (95%CI: 3,627 to 11,587) inpatient days and US$788,775 (95%CI: $349,272 to $1,115,825) cost were attributable to PM exposure.

Conclusions: Long-term exposure to ambient PM was positively associated with hospitalization for many cancer types in Brazil. Inpatient days and cost would be saved if the annual PM exposure was reduced.
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http://dx.doi.org/10.1016/j.envint.2021.106671DOI Listing
June 2021

Association between air particulate matter pollution and blood cell counts of women preparing for pregnancy: Baseline analysis of a national birth cohort in China.

Environ Res 2021 May 30;200:111399. Epub 2021 May 30.

Environmental and Spatial Epidemiology Research Center, National Human Genetic Resources Center, Beijing, China; National Research Institute for Family Planning, Beijing, China. Electronic address:

Background: Limited evidence is known about whether long-term exposures to air borne particulate matters of 2.5 μm or less (PM) impact human hematologic index for women preparing for pregnancy. No study assessed the effect of PM, which is small enough to reach the blood circulation.

Objective: To evaluate whether exposure to PM and PM is associated with blood cell count of woman preparing for pregnancy.

Method: Based on the baseline data of a national birth cohort in China, we analysed the white blood cell (WBC), red blood cells (RBC) and thrombocyte counts of 1,203,565 women who are aged 18-45 years, being Han ethnicity, had no chronic disease and preparing for pregnancy. We matched their home addresses and examination date with daily concentrations of PM and PM which were estimated by a machine learning method with remote sensing, meteorological and land use information. Generalized additive mixed model to examine the associations between exposure to one-year average exposure to PMs prior to the health examination and the blood cells counts, after adjustment for potential individual variables.

Results: A 10 μg/m PM increment was associated with -1.49% (95%CI: 1.56%, -1.42%) difference in WBC count; with 0.33% (95%CI: 0.30%, 0.36%) difference of RBC count; and with 1.08% (95%CI: 1.01%, 1.15%) difference of thrombocyte count. For PM, the corresponding difference was -0.47% (95%CI: 0.54%, -0.39%) for WBC; was 0.06% (95%CI: 0.03%, 0.09%) for RBC; and was 1.10% (95%CI: 1.02%, 1.18%) for thrombocyte. Women working as workers, being overweight and with tobacco smoking exposure had higher associations between PMs and hematologic index than their counterparts (p < 0.05 for interaction test).

Conclusion: Long-term exposure to PMs were associated with decrement in WBC, as well as increment in RBC and thrombocytes among Han Chinese women preparing for pregnancy. Measures such as using air purifiers and wearing a mask in polluted areas should be improved to prevent women from the impact of PMs.
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http://dx.doi.org/10.1016/j.envres.2021.111399DOI Listing
May 2021

Large-Scale Spraying of Roads with Water Contributes to, Rather Than Prevents, Air Pollution.

Toxics 2021 May 28;9(6). Epub 2021 May 28.

International Laboratory for Air Quality and Health, Queensland University of Technology, 2 George Street, Brisbane, QLD 4000, Australia.

Spraying roads with water on a large scale in Chinese cities is one of the supplementary precaution or mitigation actions implemented to control severe air pollution events or heavy haze-fog events in which the mechanisms causing them are not yet fully understood. These air pollution events were usually characterized by higher air humidity. Therefore, there may be a link between this action and air pollution. In the present study, the impact of water spraying on the PM concentration and humidity in air was assessed by measuring chemical composition of the water, undertaking a simulated water spraying experiment, measuring residues and analyzing relevant data. We discovered that spraying large quantities of tap or river water on the roads leads to increased PM concentration and humidity, and that daily continuous spraying produces a cumulative effect on air pollution. Spraying the same amount of water produces greater increases in humidity and PM concentration during cool autumn and winter than during hot summer. Our results demonstrate that spraying roads with water increases, rather than decreases, the concentration of PM and thus is a new source of anthropogenic aerosol and air pollution. The higher vapor content and resultant humidity most likely create unfavorable meteorological conditions for the dispersion of air pollution in autumn and winter with low temperature.
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http://dx.doi.org/10.3390/toxics9060122DOI Listing
May 2021

Chinese Propolis Suppressed Pancreatic Cancer Panc-1 Cells Proliferation and Migration via Hippo-YAP Pathway.

Molecules 2021 May 10;26(9). Epub 2021 May 10.

College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.

Pancreatic cancer is one of the most malignant cancers with high mortality. Therefore, it is of great urgency to develop new agents that could improve the prognosis of Pancreatic cancer patients. Chinese propolis (CP), a flavonoid-rich beehive product, has been reported to have an anticancer effect. In this study, we applied CP to the human Pancreatic cancer cell line Panc-1 to verify its impact on tumor development. CP induced apoptosis in Panc-1 cells from 12.5 µg/mL in a time- and dose-dependent manner with an IC value of approximately 50 µg/mL. Apoptosis rate induced by CP was examined by Annexing FITC/PI assay. We found that 48 h treatment with 50 µg/mL CP resulted in 34.25 ± 3.81% apoptotic cells, as compared to 9.13 ± 1.76% in the control group. We further discovered that the Panc-1 cells tended to be arrested at G2/M phase after CP treatment, which is considered to contribute to the anti-proliferation effect of CP. Furthermore, our results demonstrated that CP suppressed Panc-1 cell migration by regulating epithelial-mesenchymal transition (EMT). Interestingly, the Hippo pathway was activated in Panc-1 cells after CP treatment, serving as a mechanism for the anti-pancreatic cancer effect of CP. These findings provide a possibility of beehive products as an alternative treatment for pancreatic cancer.
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http://dx.doi.org/10.3390/molecules26092803DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8126155PMC
May 2021

Suppression of Esophageal Squamous Cell Carcinoma Development by Mechanosensitive Protein Piezo1 Downregulation.

ACS Omega 2021 Apr 12;6(15):10196-10206. Epub 2021 Apr 12.

BGI College & Henan Institute of Medical and Pharmaceutical Sciences, Zhengzhou University, No. 40 Daxue Road, Zhengzhou 450052, China.

Esophageal squamous cell carcinoma (ESCC) is a malignant epithelial cancer of the esophageal epithelium. Piezo-type mechanosensitive ion channel component 1 (Piezo1), an essential mechanosensitive protein, plays an important role in maintaining cell biological functions under the stimulation of physiological force. Immunohistochemical and bioinformatic analyses of ESCC tissue samples indicate that Piezo1 expression is higher in ESCC tissues than in paracancerous tissues. shRNA-mediated Piezo1 downregulation in the ESCC lines EC9706 and EC109 showed that proliferation, migration, and invasion were suppressed by Piezo1 knockdown. Piezo1 downregulation suppresses ESCC migration and invasion in both cells and tissues via the epithelial-mesenchymal transition pathway. Moreover, G0/G1 to S-phase cell cycle progression was inhibited, and cell apoptosis was induced by Piezo1 downregulation. Furthermore, we observed an interaction between Piezo1 and p53 using immunoprecipitation. The protein levels of p53, downstream factor Bax, apoptosis executioner cleaved-caspase3, and caspase3 were significantly upregulated by the downregulation of Piezo1. The inhibited growth rate and upregulated expression of these related factors were validated using tumor-bearing mice. Therefore, Piezo1 downregulation induces ESCC apoptosis via a Piezo1-p53-Bax-Caspase 3 axis. In conclusion, Piezo1 downregulation suppresses ESCC development, and mechanosensitive protein Piezo1 can be considered a new target for ESCC therapy.
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http://dx.doi.org/10.1021/acsomega.1c00505DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153669PMC
April 2021

Exercise ameliorates aberrant synaptic plasticity without enhancing adult-born cell survival in the hippocampus of serotonin transporter knockout mice.

Brain Struct Funct 2021 Jul 30;226(6):1991-1999. Epub 2021 May 30.

Florey Institute of Neuroscience and Mental Health, Melbourne Brain Centre, University of Melbourne, Parkville, Melbourne, VIC, 3010, Australia.

Deficits in hippocampal cellular and synaptic plasticity are frequently associated with cognitive and mood disorders, and indeed common mechanisms of antidepressants are thought to involve neuroplastic processes. Here, we investigate hippocampal adult-born cell survival and synaptic plasticity (long-term potentiation, LTP, and long-term depression, LTD) in serotonin transporter (5-HTT) knockout (KO) mice. From 8 weeks of age, mice either continued in standard-housing conditions or were given access to voluntary running wheels for 1 month. Electrophysiology was performed on hippocampal slices to measure LTP and LTD, and immunohistochemistry was used to assess cell proliferation and subsequent survival in the dentate gyrus. The results revealed a reduced LTP in 5-HTT KO mice that was restored to wild-type (WT) levels after chronic exercise. While LTD appeared normal in 5-HTT KO, exercise decreased the magnitude of LTD in both WT and 5-HTT KO mice. Furthermore, although 5-HTT KO mice had normal hippocampal adult-born cell survival, they did not benefit from the pro-proliferative effects of exercise observed in WT animals. Taken together, these findings suggest that reduced 5-HTT expression is associated with significant alterations to functional neuroplasticity. Interestingly, 5-HTT appeared necessary for exercise-induced augmentation of adult-born hippocampal cell survival, yet exercise corrected the LTP impairment displayed by 5-HTT KO mice. Together, our findings further highlight the salience of serotonergic signalling in mediating the neurophysiological benefits of exercise.
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http://dx.doi.org/10.1007/s00429-021-02283-yDOI Listing
July 2021

Air pollution control efficacy and health impacts: A global observational study from 2000 to 2016.

Environ Pollut 2021 May 3;287:117211. Epub 2021 May 3.

School of Public Health and Management, Binzhou Medical University, Yantai, Shandong Province, 264003, PR China; School of Public Health and Preventive Medicine, Monash University, Melbourne, VIC, 3004, Australia. Electronic address:

Particulate matter with aerodynamic diameter ≤2.5 μm (PM) concentrations vary between countries with similar carbon dioxide (CO) emissions, which can be partially explained by differences in air pollution control efficacy. However, no indicator of air pollution control efficacy has yet been developed. We aimed to develop such an indicator, and to evaluate its global and temporal distribution and its association with country-level health metrics. A novel indicator, ambient population-weighted average PM concentration per unit per capita CO emission (PM/CO), was developed to assess country-specific air pollution control efficacy (abbreviated as APCI). We estimated and mapped the global average distribution of APCI and its changes during 2000-2016 across 196 countries. Pearson correlation coefficients and Generalized Additive Mixed Model (GAMM) were used to evaluate the relationship between APCI and health metrics. APCI varied by country with an inverse association with economic development. APCI showed an almost stable trend globally from 2000 to 2016, with the low-income groups increased and several countries (China, India, Bangladesh) decreased. The Pearson correlation coefficients between APCI and life expectancy at birth (LE), infant-mortality rate (IMR), under-five year of age mortality rate (U5MR) and logarithm of per capita GDP (LPGDP) were -0.57, 0.65, 0.66, -0.59 respectively (all P values < 0.001). APCI could explain international variation of LE, IMR and U5MR. The associations between APCI and LE, IMR, U5MR were independent of per capita GDP and climatic factors. We consider APCI to be a good indicator for air pollution control efficacy given its relation to important population health indicators. Our findings provide a new metric to interpret health inequity across the globe from the point of climate change and air pollution control efficacy.
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http://dx.doi.org/10.1016/j.envpol.2021.117211DOI Listing
May 2021

Discovery of STAT3 and Histone Deacetylase (HDAC) Dual-Pathway Inhibitors for the Treatment of Solid Cancer.

J Med Chem 2021 06 27;64(11):7468-7482. Epub 2021 May 27.

Jiangsu Key Laboratory of Bioactive Natural Product Research and State Key Laboratory of Natural Medicines, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, People's Republic of China.

Nowadays, simultaneous inhibition of multiple targets through drug combination is an important anticancer strategy owing to the complex mechanism behind tumorigenesis. Recent studies have demonstrated that the inhibition of histone deacetylases (HDACs) will lead to compensated activation of a notorious cancer-related drug target, signal transducer and activator of transcription 3 (STAT3), in breast cancer through a cascade, which probably limits the anti-proliferation effect of HDAC inhibitors in solid tumors. By incorporating the pharmacophore of the HDAC inhibitor SAHA (vorinostat) into the STAT3 inhibitor pterostilbene, a series of potent pterostilbene hydroxamic acid derivatives with dual-target inhibition activity were synthesized. An excellent hydroxamate derivate, compound , inhibited STAT3 ( = 33 nM) and HDAC (IC = 23.15 nM) with robust potency . Compound also showed potent anti-proliferation ability and . Our study provides the first STAT3 and HDAC dual-target inhibitor for further exploration.
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http://dx.doi.org/10.1021/acs.jmedchem.1c00136DOI Listing
June 2021

The predictive power of saliva electrolytes exceeds that of saliva microbiomes in diagnosing early childhood caries.

J Oral Microbiol 2021 May 13;13(1):1921486. Epub 2021 May 13.

School of Stomatology, Qingdao University, Qingdao, Shandong, China.

Early childhood caries (ECC) is one of the most prevalent chronic diseases affecting children worldwide, and thus its etiology, diagnosis, and prognosis are of particular clinical significance. This study aims to test the ability of salivary microbiome and electrolytes in diagnosing ECC, and their interplays within the same population. We here simultaneously profiled salivary microbiome and biochemical components of 331 children (166 caries-free (H group) and 165 caries-active children (C group)) aged 4-6 years. We identified both salivary microbial and biochemical dysbiosis associated with ECC. Remarkably, K, Cl, NH, Na, SO, Ca, Mg, and Br were enriched while pH and NO were depleted in ECC. Moreover, the dmft index (ECC severity) positively correlated with Cl, NH, Ca, Mg, Br, while negatively with pH and NO. Furthermore, machine-learning classification models were constructed based on these biomarkers from saliva microbiota, or electrolytes (and pH). Unexpectedly, the electrolyte-based classifier (AUROC = 0.94) outperformed microbiome-based (AUROC = 0.70) one and the composite-based one (with both microbial and biochemical data; AUC = 0.89) in predicting ECC. Collectively, these findings indicate ECC-associated alterations and interplays in the oral microbiota, electrolytes and pH, underscoring the necessity of developing diagnostic models with predictors from salivary electrolytes.
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http://dx.doi.org/10.1080/20002297.2021.1921486DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8131007PMC
May 2021

Synergistic effects of myogenic cells and fibroblasts on the promotion of engineered tendon regeneration with muscle derived cells.

Connect Tissue Res 2021 May 24:1-10. Epub 2021 May 24.

Department of Plastic and Reconstructive Surgery, Plastic Surgery Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing PR China.

Aims: Tendon development requires the coordinated interaction of muscles and tendons. Muscle-derived cells (MDCs), a mixed cell population containing both myogenic and fibroblastic cell subsets, have been found to be ideal seed cells for tendon regeneration. However, the necessity of these cell types for tendon regeneration has not yet been tested. In this study, we aim to explore the possible synergistic effects of myogenic cells and fibroblasts in engineered tendon regeneration.

Methods: MDCs were separated into rapidly adhering cell (RAC; fibroblasts) and slowly adhering cell (SAC; myogenic cells) populations. Myogenic- and tenogenic-related molecules were analyzed by immunofluorescent staining, RT-PCR and real-time PCR. The proliferative abilities of MDCs, RACs and SACs were also evaluated. Cell-scaffold constructs were implanted into nude mice, and subsequently evaluated for their histologic, ultrastructure, gene expression, and biomechanical characteristics.

Results: MDCs have better proliferative activity than RAC and SAC population. RACs could express higher levels of tenogenic-related molecules tenomodulin (TNMD) and scleraxis (SCX) than SACs. Whereas SACs only expressed myogenic-related molecules MyoD. In contrast to the tendons engineered using RACs and SACs, the tendons engineered using MDCs exhibited a relatively more mature and well-organized tissue structure and ultrastructure as well as better mechanical properties.

Conclusions: Fibroblasts in muscle may be the primary cell population involved in tendon regeneration and that myogenic cells are an important component of the niche and control the fibroblast activity during tendon regeneration. The synergistic effects between fibroblasts and myogenic cells significantly contribute to efficient and effective regeneration of engineered tendons.
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http://dx.doi.org/10.1080/03008207.2021.1924158DOI Listing
May 2021

Retraction Note to: A new synthetic derivative of cryptotanshinone KYZ3 as STAT3 inhibitor for triple-negative breast cancer therapy.

Cell Death Dis 2021 May 21;12(6):522. Epub 2021 May 21.

State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, 210009, Nanjing, China.

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http://dx.doi.org/10.1038/s41419-021-03809-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139949PMC
May 2021

Longitudinal Development of Refractive Error in Children Treated With Intravitreal Bevacizumab or Laser for Retinopathy of Prematurity.

Transl Vis Sci Technol 2021 Apr;10(4):14

Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, TX, USA.

Purpose: To compare the patterns of longitudinal refractive error development during the first 3.5 years in children with severe retinopathy of prematurity (ROP) treated with intravitreal bevacizumab (IVB) or laser photocoagulation.

Methods: This prospective cohort study enrolled extremely preterm infants (birth weight < 1000 g, gestational age 23-27 weeks) with type 1 ROP from multiple hospitals in Dallas between 1999 and 2017; IVB group (N = 22); laser group (N = 26). Cycloplegic retinoscopy was conducted from 0.04 years corrected age and every 0.5 to 1.0 years thereafter until 3.5 years old. Right eye spherical equivalent (SEQ) and astigmatism, anisometropia, and better-eye visual acuity were analyzed over time.

Results: In all children, both eyes were treated with the same modality. At the final visit, the prevalence of myopia (SEQ ≤ -1D) was 82.7% in the laser group and 47.7% in the IVB group (P < 0.05) with a mean SEQ of -8.0D ± 5.8D in the laser group versus -2.3D ± 4.2D in the IVB group (P < 0.001). Longitudinal SEQ were best fit with a bilinear model. Before one year, the rate of SEQ change was -5.0D/year in the laser group, but only -3.5D/year in the IVB group (T = -5.14, P < 0.001); after one year, there was a significant flattening of these slopes (T = 6.23, P < 0.001). Anisometropia in the IVB group was significantly less than in the laser group (P < 0.05). Final visual acuity in both groups was similar at 0.47 logMAR (∼ 20/60).

Conclusions: Children with severe ROP treated with IVB developed less myopic refractive error than those treated with laser largely because of a slower rate of refractive change during the first year of life.

Translational Relevance: These findings may inform decisions regarding ROP treatment timing and modality.
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http://dx.doi.org/10.1167/tvst.10.4.14DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8054622PMC
April 2021

Dietary Pattern and Long-Term Effects of Particulate Matter on Blood Pressure: A Large Cross-Sectional Study in Chinese Adults.

Hypertension 2021 Jul 17;78(1):184-194. Epub 2021 May 17.

West China School of Public Health, West China Fourth Hospital, Sichuan University, Chengdu, China (H.X., B.G., X.X., J. Wu, X.W., X.Z.).

[Figure: see text].
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http://dx.doi.org/10.1161/HYPERTENSIONAHA.121.17205DOI Listing
July 2021

Associations of residential greenness with hypertension and blood pressure in a Chinese rural population: a cross-sectional study.

Environ Sci Pollut Res Int 2021 May 14. Epub 2021 May 14.

Department of Global Health, School of Health Sciences, Wuhan University, Wuhan, Hubei, China.

Limited epidemiological literature identified the associations between residential greenness and hypertension in low-/middle-income countries. A random sampling strategy was adopted to recruit 39,259 residents, ≥ 18 years, and from 5 counties in central China. Blood pressure was measured based on the protocol of the American Heart Association. Hypertension was defined according to the 2010 Chinese guidelines for the management of hypertension. The satellite-derived normalized difference vegetation index (NDVI) and enhanced vegetation index (EVI) were applied to estimate the residential greenness. Mixed logit model and mixed linear model were utilized to explore the relationships of residential greenness with hypertension and blood pressure. Higher residential greenness was associated with lower odds of hypertension and blood pressure levels. For instance, an interquartile range (IQR) increase in NDVI was linked with lower odds of hypertension (OR = 0.92, 95%CI 0.88 to 0.95), a decrease of -0.88 mm Hg (95% CI -1.17 to -0.58) and -0.64 mm Hg (95% CI -0.82 to -0.46) in SBP and DBP, respectively. The effect of residential greenness was more pronounced in males, smokers, and drinkers. Long-term exposure to residential greenness was linked with lower odds of hypertension. More prospective studies are needed to verify the hypothesis.
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http://dx.doi.org/10.1007/s11356-021-14201-0DOI Listing
May 2021

Characterization of Brain Microstructural Abnormalities in High Myopia Patients: A Preliminary Diffusion Kurtosis Imaging Study.

Korean J Radiol 2021 May 4. Epub 2021 May 4.

Department of Radiology, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

Objective: To evaluate microstructural damage in high myopia (HM) patients using 3T diffusion kurtosis imaging (DKI).

Materials And Methods: This prospective study included 30 HM patients and 33 age- and sex-matched healthy controls (HCs) with DKI. Kurtosis parameters including kurtosis fractional anisotropy (FA), mean kurtosis (MK), axial kurtosis (AK), and radial kurtosis (RK) as well as diffusion metrics including FA, mean diffusivity, axial diffusivity (AD), and radial diffusivity derived from DKI were obtained. Group differences in these metrics were compared using tract-based spatial statistics. Partial correlation analysis was used to evaluate correlations between microstructural changes and disease duration.

Results: Compared to HCs, HM patients showed significantly reduced AK, RK, MK, and FA and significantly increased AD, predominately in the bilateral corticospinal tract, right inferior longitudinal fasciculus, superior longitudinal fasciculus, inferior fronto-occipital fasciculus, and left thalamus (all < 0.05, threshold-free cluster enhancement corrected). In addition, DKI-derived kurtosis parameters (AK, RK, and MK) had negative correlations ( = -0.448 to -0.376, all < 0.05) and diffusion parameter (AD) had positive correlations ( = 0.372 to 0.409, all < 0.05) with disease duration.

Conclusion: HM patients showed microstructural alterations in the brain regions responsible for motor conduction and vision-related functions. DKI is useful for detecting white matter abnormalities in HM patients, which might be helpful for exploring and monitoring the pathogenesis of the disease.
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http://dx.doi.org/10.3348/kjr.2020.0178DOI Listing
May 2021

Interplay Between Glucose Metabolism and Chromatin Modifications in Cancer.

Front Cell Dev Biol 2021 27;9:654337. Epub 2021 Apr 27.

State Key Laboratory of Biocatalysis and Enzyme Engineering, Environmental Microbial Technology Center of Hubei, School of Life Sciences, Hubei University, Wuhan, China.

Cancer cells reprogram glucose metabolism to meet their malignant proliferation needs and survival under a variety of stress conditions. The prominent metabolic reprogram is aerobic glycolysis, which can help cells accumulate precursors for biosynthesis of macromolecules. In addition to glycolysis, recent studies show that gluconeogenesis and TCA cycle play important roles in tumorigenesis. Here, we provide a comprehensive review about the role of glycolysis, gluconeogenesis, and TCA cycle in tumorigenesis with an emphasis on revealing the novel functions of the relevant enzymes and metabolites. These functions include regulation of cell metabolism, gene expression, cell apoptosis and autophagy. We also summarize the effect of glucose metabolism on chromatin modifications and how this relationship leads to cancer development. Understanding the link between cancer cell metabolism and chromatin modifications will help develop more effective cancer treatments.
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http://dx.doi.org/10.3389/fcell.2021.654337DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8110832PMC
April 2021

Corrigendum "Evaluation of chemical cross-linkers for in-depth structural analysis of G protein-coupled receptors through cross-linking mass spectrometry" [Analytica Chimica Acta 1102 (2020) 53-62].

Anal Chim Acta 2021 Jun 8;1165:338471. Epub 2021 Apr 8.

iHuman Institute, ShanghaiTech University, 201210, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, 201210, Shanghai, China. Electronic address:

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http://dx.doi.org/10.1016/j.aca.2021.338471DOI Listing
June 2021

Long-term exposure to particulate matter and residential greenness in relation to androgen and progesterone levels among rural Chinese adults.

Environ Int 2021 Aug 4;153:106483. Epub 2021 May 4.

Department of Epidemiology and Biostatistics, College of Public Health, Zhengzhou University, Zhengzhou, Henan, PR China. Electronic address:

Background: Population-based studies on the associations of long-term exposure to particulate matter (PM) with androgen and progesterone are still scant. Residential greenness is benefits health by promoting physical activity, reducing air pollution, and improving mental health, but it remains unclear whether it is related to androgen and progesterone levels among humans.

Aims: This study aimed to explore the individual and interactive effects of PM and residential greenness on serum testosterone and progesterone levels among rural Chinese adults.

Methods: A total of 6017 subjects were recruited from the baseline of the Henan Rural Cohort Study in 2016. Serum testosterone and progesterone were measured with liquid chromatography-tandem mass spectrometry. Particulate matters (PM) (PM, PM, and PM) were assessed by machine learning algorithms. Residential greenness was assessed using the normalized difference vegetation index (NDVI) within 500-m, 1000-m, and 3000-m buffers around participants' residences. The effects of air pollutants and residential greenness and their interaction on serum testosterone and progesterone levels were assessed using linear mixed-effects models with township as a random intercept.

Results: After adjusting for potential confounding factors, a 1 μg/m increase in PM or PM was associated with a 0.037 or 0.030 ng/ml increase in serum testosterone, respectively, in females and with a 0.111 or 0.182 ng/ml decrease in serum progesterone, respectively, in males. A 1 μg/m increase in PM, PM or PM was associated with a 0.222, 0.306, or 0.295 ng/ml decrease in serum progesterone, respectively, among females. Moreover, a 0.1-unit increase in NDVI was associated with a 0.310 ng/ml increase in serum testosterone and a 0.170 ng/ml increased in serum progesterone in males, as well as with a 0.143 ng/ml increase in serum progesterone in females. Interaction effects of PM and residential greenness on serum testosterone and progesterone levels were observed, indicating that the effects of residential greenness on serum testosterone and progesterone were modified by high levels of PM. In addition, physical activity significantly mediated 2.92% of the estimated association between greenness and testosterone levels.

Conclusions: Our study suggested that long-term exposure to PM was positively associated with serum testosterone in males but negatively associated with progesterone levels in both genderssin. In addition, positive associations of residential greenness with serum testosterone and progesterone levels were observed, but they were modified by high levels of PM. Furthermore, the estimated effects of residential greenness on testosterone levels were partly mediated by physical activity.
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http://dx.doi.org/10.1016/j.envint.2021.106483DOI Listing
August 2021

Impact of arabinoxylan on characteristics, stability and lipid oxidation of oil-in-water emulsions: Arabinoxylan from wheat bran, corn bran, rice bran, and rye bran.

Food Chem 2021 Oct 20;358:129813. Epub 2021 Apr 20.

College of Food Science, Sichuan Agricultural University, No.46 Xinkang Road, Yucheng District, Yaan, Sichuan 625014, China. Electronic address:

To investigate the impact of arabinoxylan (AX) on the physical and oxidative stability of oil-in-water emulsions, AX from wheat bran, corn bran, rice bran, or rye bran was incorporated into the production of whey protein isolate-stabilised emulsions. Decreased interfacial charge and increased mean particle diameters were recorded in all fresh emulsions with 0.1%-0.5% AX, as recorded by the ζ-potential and particle size measurement, indicating the adsorption of AX onto the oil droplets. No phase separation was observed in all emulsions with ≤0.3% AX after 14-day storage in dark. Spectrophotometric analysis demonstrated that all AX lowered the peroxide value and thiobarbituric acid reactive substance concentration in emulsions, with AX from rice bran being slightly more effective. Consequently, AX has the potential to be used as a natural interfacial antioxidant in emulsions, and the antioxidant capacity of AX varies with its source.
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http://dx.doi.org/10.1016/j.foodchem.2021.129813DOI Listing
October 2021

Study on Material Removal Model by Reciprocating Magnetorheological Polishing.

Micromachines (Basel) 2021 Apr 8;12(4). Epub 2021 Apr 8.

School of Mechanical Engineering and Automation, Northeastern University, Shenyang 110819, China.

In this study, a new reciprocating magnetorheological polishing (RMRP) method for a flat workpiece was proposed. Based on the RMRP principle and Preston equation, the material removal rate (MRR) model of the RMRP as well as its normal polishing pressure model was established. On this basis, the effects of different technological parameters including workpiece rotation speed, eccentric wheel rotation speed and eccentricity on the MRR of the workpiece were investigated. The K9 optical flat glass was polished with the RMRP setup to verify the MRR model. The experimental results showed that the effect of workpiece rotation speed on the MRR was much greater than that of eccentric wheel rotation speed and eccentricity, and the MRR increased from 0.0115 ± 0.0012 to 0.0443 ± 0.0015 μm/min as workpiece rotation speed rose. The optimum surface roughness reduced to Ra 50.8 ± 1.2 from initial Ra 330.3 ± 1.6 nm when the technical parameters of the workpiece rotation speed of 300 rpm, the eccentric wheel rotation speed of 20 rpm and the eccentricity of 0.02 m were applied. The average relative errors between the theoretical and experimental values were 16.77%, 10.59% and 7.38%, respectively, according to the effects of workpiece rotation speed, eccentric wheel rotation speed and eccentricity on MRR.
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http://dx.doi.org/10.3390/mi12040413DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8068226PMC
April 2021

Sand and dust storms in Asia: a call for global cooperation on climate change.

Lancet Planet Health 2021 Jun 27;5(6):e329-e330. Epub 2021 Apr 27.

School of Public Health and Preventive Medicine, Monash University, Melbourne, VIC 3004, Australia. Electronic address:

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http://dx.doi.org/10.1016/S2542-5196(21)00082-6DOI Listing
June 2021

An enzyme-responsive Gp1a-hydrogel for skin wound healing.

J Biomater Appl 2021 Apr 28:8853282211012870. Epub 2021 Apr 28.

Jiangsu Medical Engineering Research Center of Gene Detection and Department of Forensic Medicine, Xuzhou Medical University, Xuzhou, China.

Faster recovery and fewer scars are ideal wound healing. We have demonstrated that the cannabinoid receptor 2 (CB) agonist Gp1a is beneficial to skin wound healing, which inhibits inflammation and fibrogenesis while promoting re-epithelialization. However, the systemic administration is imprecise and overqualified for a local skin wound. Herein, we prepared Gp1a-gel using triglycerol monostearate (Tm) hydrogel and detected whether the Gp1a-gel worked effectively on mouse skin excision wounds. The results showed that Gp1a-gel might sustainably increase the CB for at least 8 days. It decreased inflammation and fibrogenesis while promoting wound enclosure and re-epithelialization. These results suggested Gp1a-gel may utilize as a potential formulation strategy to treat the skin wound.
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http://dx.doi.org/10.1177/08853282211012870DOI Listing
April 2021

Molecular analyses of the diversity and function of the family 1 β-glucosidase-producing microbial community in compost.

Can J Microbiol 2021 Apr 27. Epub 2021 Apr 27.

Northeast Agricultural University, 12430, Harbin, China, 150030;

The diversity and transcription efficiency of GH1 family β-glucosidase genes were investigated in natural and inoculated composts using a DNA clone library and real-time qPCR. Compositional differences were observed in the functional community between both composting processes. Proteobacteria, Actinobacteria, Firmicutes, and Chloroflexi were the dominant phyla. Twenty representative β-glucosidase genes were quantitatively analyzed from DNA and RNA pools. Principal component analysis and Pearson's correlation analysis showed that cellulose degradation is correlated with the composition and succession of functional microbial communities, and this correlation was mainly observed in Proteobacteria and Actinobacteria. Compared with inoculated compost, the functional microbial communities in natural compost with a low diversity index exhibited weak buffering capacity for function in response to environmental changes. This may explain the consistency and dysfunction of cellulose degradation and transcriptional regulation by dominant β-glucosidase genes. Except for the β-glucosidase genes encoding constitutive enzymes, individual β-glucosidase genes responded to environmental changes more drastically than the group β-glucosidase genes. Correlation results suggested that β-glucosidase genes belonging to Micrococcales played an important role in the regulation of intracellular β-glucosidase. These results indicated that the responses of functional microorganisms were different during both composting processes, and were reflected at both the individual and group levels.
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http://dx.doi.org/10.1139/cjm-2020-0576DOI Listing
April 2021

Association of long-term exposure to ambient air pollutants with blood lipids in Chinese adults: The China Multi-Ethnic Cohort study.

Environ Res 2021 Jun 22;197:111174. Epub 2021 Apr 22.

West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, China. Electronic address:

Background: Dyslipidemia is a crucial risk factor for cardiovascular diseases. Previous studies have suggested that air pollution is associated with blood lipids. However, little evidence exists in low- and middle-income regions. We aimed to investigate the association between air pollution and blood lipids in southwestern China.

Methods: We included 67,305 participants aged 30-79 years from the baseline data of the China Multi-Ethnic Cohort (CMEC) study. Three-year average concentrations of particles with diameters ≤1 μm (PM), particles with diameters ≤ 2.5 μm (PM), particles with diameters ≤ 10 μm (PM), nitrogen dioxide (NO), and ozone (O) were estimated using satellite-based spatiotemporal models. Individual serum lipids, including cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C), were measured. Linear, logistic, and quantile regression models were used to evaluate the association between ambient air pollution and blood lipids.

Results: All five air pollutants in our study were associated with lipid levels. Increased air pollution exposure was associated with a high risk of dyslipidemia. Each 10 μg/m increase in PM was associated with 0.92% (95% confidence interval (CI): 0.64%, 1.20%), 2.23% (95% CI: 1.44%, 3.02%), and 3.04% (95% CI: 2.61%, 3.47%) increases in TC, TG, and LDL-C levels, respectively, and a 2.03% (95% CI: 1.69%, 2.37%) decrease in HDL-C levels, and high risks of dyslipidemia (OR = 1.14, 95% CI: 1.10, 1.18). Stronger associations of air pollution with blood lipids were found in participants with high lipid levels than in those with low lipid levels.

Conclusion: Long-term exposure to air pollutants was associated with blood lipid levels and the risk of dyslipidemia. People with high lipid levels were more susceptible to air pollution. Therefore, air pollution prevention and control may help reduce the incidence of dyslipidemia and the burden of CVDs.
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http://dx.doi.org/10.1016/j.envres.2021.111174DOI Listing
June 2021