Publications by authors named "Shan Shan Li"

307 Publications

hsa_circNFXL1_009 modulates apoptosis, proliferation, migration, and potassium channel activation in pulmonary hypertension.

Mol Ther Nucleic Acids 2021 Mar 28;23:1007-1019. Epub 2020 Sep 28.

School of Medicine, Nankai University, Tianjin, China.

In this study, we explored the circular RNA (circRNA) profile in pulmonary arterial hypertension (PAH) patients caused by chronic obstructive pulmonary disease (COPD) and the effects of hsa_circNFXL1_009 on abnormal proliferation, apoptosis, and migration of human pulmonary arterial smooth muscle cells (hPASMCs) driven by hypoxia. Using microarrays, we screened the circRNA profile in whole-blood samples from three pairs of subjects and found 158 dysregulated circRNAs in patients with PAH-COPD. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) analysis further validated that hsa_circNFXL1_009 was dramatically downregulated with the highest area under a receiver operating characteristic curve (ROC) in 21 pairs of subjects. Consistently, exposure to hypoxia markedly reduced the hsa_circNFXL1_009 level in cultured hPASMCs. Delivery of exogenous hsa_circNFXL1_009 attenuated hypoxia-induced proliferation, apoptotic resistance, and migration of hPASMCs, as evidenced by immunocytochemistry, 5-ethynyl-2'-deoxyuridine incorporation, wound healing, and a TUNEL (terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling) assay. A luciferase assay showed that hsa_circNFXL1_009 directly sponged hsa-miR-29b-2-5p (miR-29b) and positively regulated the expression of voltage-gated potassium (K) channel subfamily B member 1 () at the mRNA level. Using patch-clamp electrophysiology, we proved that overexpression of hsa_circNFXL1_009 promoted a whole-cell K current in hPASMCs. Taken together, these studies identify hsa_circNFXL1_009 as a key regulator of PAH, and it may be used as a potential therapeutic target for the treatment of PAH.
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http://dx.doi.org/10.1016/j.omtn.2020.09.029DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868929PMC
March 2021

Risk Factors for Stillbirth among Pregnant Women Infected with Syphilis in the Zhejiang Province of China, 2010-2016.

Can J Infect Dis Med Microbiol 2021 2;2021:8877962. Epub 2021 Feb 2.

Women's Hospital of Zhejiang University School of Medicine, Hangzhou, China.

Background: The World Health Organization estimated that about 1.36 million pregnant women suffered from syphilis in 2008, and nearly 66% of adverse effects occurred in those who were not tested or treated. Syphilis infection is one of the most common maternal factors associated with stillbirth.

Objective: This study aimed to determine the risk factors for stillbirth among pregnant women infected with syphilis.

Methods: In this retrospective study, data on stillbirth and gestational syphilis from 2010 to 2016 were extracted from the prevention of mother-to-child transmission (PMTCT) program database in the Zhejiang province. A total of 8,724 pregnant women infected with syphilis were included. Multiple logistic regression analysis was performed to determine the degree of association between gestational syphilis and stillbirth.

Results: We found that the stillbirth percentage among pregnant women infected with syphilis was 1.7% (152/8,724). Compared with live births, stillbirth was significantly associated with lower maternal age, not being married, lower gravidity, the history of syphilis, nonlatent syphilis stage, higher maternal serum titer for syphilis, inadequate treatment for syphilis, and later first antenatal care visit. In multiple logistic analysis, nonlatent syphilis (adjusted odds ratio (AOR) = 2.03; 95% CI = 1.17, 3.53) and maternal titers over 1 : 4 (AOR = 1.78; 95% CI = 1.25, 2.53) were risk factors for stillbirth, and adequate treatment was the only protective factor for stillbirth (AOR = 0.16; 95% CI = 0.10, 0.25).

Conclusions: Nonlatent syphilis and maternal titers over 1 : 4 were risk factors for stillbirth, and adequate treatment was the only protective factor for stillbirth.
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http://dx.doi.org/10.1155/2021/8877962DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7872764PMC
February 2021

An ultra-sensitive electrochemical sensor of Ni/Fe-LDH toward nitrobenzene with the assistance of surface functionalization engineering.

Talanta 2021 Apr 7;225:122087. Epub 2021 Jan 7.

Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, Department of materials science and engineering, Huaibei Normal University, Huaibei, 235000, PR China. Electronic address:

Hypersensitive detection of organic pollutions with high toxicity in drinking water always keeps its challenge in electroanalysis due to their low concentration and electrochemical redox inert. In this work, a novel nanomaterial modified electrode for the sensitive detection of nitrobenzene (NB) is presented, based on environmental friendly and cost-effective Ni/Fe layered double hydroxides functionalized with sodium dodecyl sulfate (Ni/Fe(SDS)-LDH). Such 2D layered composites were prepared and used to improve the sensitivity for NB detection, due to its good catalytic activity for NB reduction. Besides, the proposed electrode shows a remarkably promoted sensitivity to NB compared to Ni/Fe-LDHs modified one. It is because that the surface modifier SDS can provide more adsorption sites to significantly improve the adsorption of NB, which has been confirmed by the adsorption experiment and the characterization of Fourier transform infrared spectroscopy (FTIR). As a result, an impressive sensing behaviour is achieved at the proposed Ni/Fe(SDS)-LDHs modified electrode with a sensitivity of 15.79 μA μM cm. This work provides a promising way to build more advanced nanomaterials to electrochemical detection of organic pollution based on energetically synergizing of adsorption by surface functionalization engineering.
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http://dx.doi.org/10.1016/j.talanta.2021.122087DOI Listing
April 2021

The dopamine D1-D2DR complex in the rat spinal cord promotes neuropathic pain by increasing neuronal excitability after chronic constriction injury.

Exp Mol Med 2021 Feb 9. Epub 2021 Feb 9.

Jiangsu Key Laboratory of TCM Evaluation and Translational Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu, 211198, China.

Dopamine D1 receptor (D1DR) and D2 receptor (D2DR) are closely associated with pain modulation, but their exact effects on neuropathic pain and the underlying mechanisms remain to be identified. Our research revealed that intrathecal administration of D1DR and D2DR antagonists inhibited D1-D2DR complex formation and ameliorated mechanical and thermal hypersensitivity in chronic constriction injury (CCI) rats. The D1-D2DR complex was formed in the rat spinal cord, and the antinociceptive effects of D1DR and D2DR antagonists could be reversed by D1DR, D2DR, and D1-D2DR agonists. Gαq, PLC, and IP3 inhibitors also alleviated CCI-induced neuropathic pain. D1DR, D2DR, and D1-D2DR complex agonists all increased the intracellular calcium concentration in primary cultured spinal neurons, and this increase could be reversed by D1DR, D2DR antagonists and Gαq, IP3, PLC inhibitors. D1DR and D2DR antagonists significantly reduced the expression of p-PKC γ, p-CaMKII, p-CREB, and p-MAPKs. Levo-corydalmine (l-CDL), a monomeric compound in Corydalis yanhusuo W.T. Wang, was found to obviously suppress the formation of the spinal D1-D2DR complex to alleviate neuropathic pain in CCI rats and to decrease the intracellular calcium concentration in spinal neurons. l-CDL-induced inhibition of p-PKC γ, p-MAPKs, p-CREB, and p-CaMKII was also reversed by D1DR, D2DR, and D1-D2DR complex agonists. In conclusion, these results indicate that D1DR and D2DR form a complex and in turn couple with the Gαq protein to increase neuronal excitability via PKC γ, CaMKII, MAPK, and CREB signaling in the spinal cords of CCI rats; thus, they may serve as potential drug targets for neuropathic pain therapy.
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http://dx.doi.org/10.1038/s12276-021-00563-5DOI Listing
February 2021

Dealing with large blood vessels within a pancreatic pseudocyst discovered during endoscopic transgastric pseudocyst fenestration (with video).

Gastrointest Endosc 2021 Feb 2. Epub 2021 Feb 2.

Department of Gastroenterology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

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http://dx.doi.org/10.1016/j.gie.2021.01.031DOI Listing
February 2021

Platelet-derived microvesicles induce calcium oscillations and promote VSMC migration TRPV4.

Theranostics 2021 1;11(5):2410-2423. Epub 2021 Jan 1.

Institute of Mechanobiology& Medical Engineering, School of Life Sciences &Biotechnology, Shanghai Jiao Tong University, Shanghai, China.

Abnormal migration of vascular smooth muscle cells (VSMCs) from the media to the interior is a critical process during the intimal restenosis caused by vascular injury. Here, we determined the role of platelet-derived microvesicles (PMVs) released by activated platelets in VSMC migration. A percutaneous transluminal angioplasty balloon dilatation catheter was used to establish vascular intimal injury. Collagen I was used to activate PMVs, mimicking collagen exposure during intimal injury. To determine the effects of PMVs on VSMC migration , scratch wound healing assays were performed. Fluorescence resonance energy transfer was used to detect variations of calcium dynamics in VSMCs. Morphological results showed that neointimal hyperplasia was markedly increased after balloon injury of the carotid artery in rats, and the main component was VSMCs. PMVs significantly promoted single cell migration and wound closure . Fluorescence resonance energy transfer revealed that PMVs induced temporal and dynamic calcium oscillations in the cytoplasms of VSMCs. The influx of extracellular calcium, but not calcium from intracellular stores, was involved in the process described above. The channel antagonist GSK219 and specific siRNA revealed that a membrane calcium channel, transient receptor potential vanilloid 4 (TRPV4), participated in the calcium oscillations and VSMC migration induced by PMVs. TRPV4 participated in the calcium oscillations and VSMC migration induced by PMVs. PMVs and the related molecules might be novel therapeutic targets for vascular remodeling during vascular injury.
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http://dx.doi.org/10.7150/thno.47182DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7797689PMC
January 2021

Enzymatic basis for stepwise C-glycosylation in the formation of flavonoid di-C-glycosides in sacred lotus (Nelumbo nucifera Gaertn.).

Plant J 2021 Jan 24. Epub 2021 Jan 24.

Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.

Lotus plumule, the embryo of sacred lotus (Nelumbo nucifera) seed, contains high accumulation of secondary metabolites including flavonoids and possesses important pharmaceutical value. Flavonoid C-glycosides, which accumulate exclusively in lotus plumule, attract considerable attention due to their unique chemical structures and special bioactivities in recent decades. Besides mono-C-glycosides, lotus plumule also accumulates various kinds of di-C-glycosides by unclear mechanisms. In this study, we identified two C-glycosyltransferases (CGTs) genes by mining sacred lotus genome data and provided in vitro and in planta evidences that these two enzymes (NnCGT1 and NnCGT2, also designated as UGT708N1 and UGT708N2, respectively) exhibited CGT activity. Recombinant UGT708N1 and UGT708N2 can C-glycosylate 2-hydroxyflavanones and 2-hydroxynaringenin C-glucoside, forming flavone mono-C-glycosides and di-C-glycosides after dehydration, respectively. In addition, the above reactions were successfully catalyzed by cell-free extracts from tobacco leaves transiently expressing NnCGT1 or NnCGT2. Finally, enzyme assays using cell-free extracts of lotus plumule suggested that flavone di-C-glycosides (vicenin-1, vicenin-3, schaftoside and isoschaftoside) were biosynthesized through sequentially C-glucosylating and C-arabinosylating/C-xylosylating 2-hydroxynaringenin. Taken together, our results provide novel insights into the biosynthesis of flavonoid di-C-glycosides by proposing a new flavone C-glycosides biosynthetic pathway of N. nucifera and identifying a novel UGT (UGT708N2) that specifically catalyzes the second glycosylation, C-arabinosylating and C-xylosylating 2-hydroxynaringenin C-glucoside.
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http://dx.doi.org/10.1111/tpj.15168DOI Listing
January 2021

Palladium Catalyzed Cascade Azidation/Carbonylation of Aryl Halides with Sodium Azide for the Synthesis of Amides.

Chem Asian J 2021 Mar 2;16(5):503-506. Epub 2021 Feb 2.

School of Biotechnology and Health Sciences, Wuyi University, 529020, Jiangmen, Guangdong, P. R. China.

Amide synthesis is one of the most important transformations in organic chemistry due to their ubiquitous presence in our daily life. In this communication, a palladium catalyzed cascade azidation/carbonylation of aryl halides for the synthesis of amides was developed. Both iodo- and bromobenzene derivatives were transformed to the corresponding amides using PdCl /xantphos as the catalyst system and sodium azide as the nitrogen-source. The reaction proceeds via a cascade azidation/carbonylation process. A range of alkyl and halogen substituted amides were prepared in moderate to good yields.
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http://dx.doi.org/10.1002/asia.202001463DOI Listing
March 2021

ACTL6A Promotes the Proliferation of Esophageal Squamous Cell Carcinoma Cells and Correlates with Poor Clinical Outcomes.

Onco Targets Ther 2021 13;14:199-211. Epub 2021 Jan 13.

Department of Pathology, School of Basic Medical Sciences, Zhengzhou University and First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, People's Republic of China.

Background: ACTL6A, a regulatory subunit of ATP-dependent chromatin-remodeling complexes SWI/SNF, has been identified as a central oncogenic driver in many tumor types.

Materials And Methods: We used immunohistochemistry (IHC) to detect ACTL6A expression in esophageal squamous cell carcinoma (ESCC) tissues. Then, the effect of ACTL6A on proliferation and DNA synthesis was explored by using cell counting kit 8 (CCK8) and EdU retention assays. The potential oncogenic mechanism of ACTL6A in ESCC cells was also analyzed by flow cytometry and Western blotting. We further established an ESCC xenograft mouse model to validate the in vitro results.

Results: ACTL6A expression, localized in cancer cell nuclei, was markedly higher in ESCC tissues than in the corresponding noncancerous tissues (P<0.001) and was positively associated with tumor size, histological differentiation, T stage and tumor-node-metastasis (TNM) stage. Kaplan-Meier analysis revealed that high ACTL6A expression was significantly associated with poor overall survival (OS) (P = 0.008, HR= 2.562, 95% CI: 1.241-5.289), and decision curve analysis (DCA) demonstrated that ACTL6A could increase the clinical prognostic efficiency of the original clinical prediction model. Further in vitro experiments showed that ACTL6A knockdown led to inhibition of cell proliferation and DNA synthesis in ESCC cell lines, while overexpression of ACTL6A had the opposite effects. ACTL6A knockdown resulted in G1 phase arrest, with downregulation of cyclin D1, CDK2 and S6K1/pS6 pathway proteins and upregulation of p21 and p27, while overexpression of ACTL6A facilitated the entry of more cells into S phase with upregulated cyclin D1, CDK2 and S6K1/pS6 pathway proteins and downregulated p21 and p27. Finally, a xenograft mouse model of ESCC cells validated the results in vitro.

Conclusion: ACTL6A expression may affect the proliferation and DNA synthesis of ESCC cells by facilitating ESCC cell cycle redistribution via the S6K1/pS6 pathway. Therefore, ACTL6A may potentially become an alternative therapeutic target for ESCC.
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http://dx.doi.org/10.2147/OTT.S288807DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7812043PMC
January 2021

Evaluation of a novel Cardiac Peri-Operative Transfusion Trigger Scoring system in patients with coronary artery disease.

BMC Cardiovasc Disord 2021 Jan 19;21(1):40. Epub 2021 Jan 19.

Department of Anesthesiology, The First Affiliated Hospital of Xinjiang Medical University, 37 Liyushan South Road, Xinshi District, Urumqi, 830054, Xinjiang, China.

Background: A simple and accurate scoring system to guide perioperative blood transfusion in patients with coronary artery disease (CAD) undergoing cardiac surgery is lacking. The trigger point for blood transfusions for these patients may be different from existing transfusion guidelines. This study aimed to evaluate the safety and efficacy of a new scoring strategy for use in guiding transfusion decisions in patients with CAD.

Methods: A multicenter randomized controlled trial was conducted at three third-level grade-A hospitals from January 2015 to May 2018. Data of 254 patients in a Cardiac Peri-Operative Transfusion Trigger Score (cPOTTS) group and 246 patients in a group receiving conventional evaluation of the need for transfusion (conventional group) were analysed. The requirements for transfusion and the per capita consumption of red blood cells (RBCs) were compared between groups.

Results: Baseline characteristics of the two groups were comparable. Logistic regression analyses revealed no significant differences between the two groups in primary outcomes (1-year mortality and perioperative ischemic cardiac events), secondary outcomes (shock, infections, and renal impairment), ICU admission, and ICU stay duration. However, patients in the cPOTTS group had significantly shorter hospital stays, lower hospital costs, lower utilization rate and lower per capita consumption of transfused RBCs than controls. Stratified analyses revealed no significant differences between groups in associations between baseline characteristics and perioperative ischemic cardiac events, except for hemofiltration or dialysis and NYHA class in I.

Conclusions: This novel scoring system offered a practical and straightforward guideline of perioperative blood transfusion in patients with CAD. Trial registration chiCTR1800016561(2017/7/19).
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http://dx.doi.org/10.1186/s12872-021-01854-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7814977PMC
January 2021

Zero-valent iron nanomaterial Fe@FeMnO for ultrasensitive electroanalysis of As(iii): Fe influenced surficial redox potential.

Chem Commun (Camb) 2021 Feb 11;57(11):1324-1327. Epub 2021 Jan 11.

Key Laboratory of Environmental Optics and Technology, and Environmental Materials and Pollution Control Laboratory, Institute of Solid State Physics, HFIPS, Chinese Academy of Sciences, Hefei 230031, China.

A novel zero-valent iron nanomaterial (Fe@FeMnO) was synthesized and achieved an ultrasensitive electrochemical detection of As(iii). It was found that the enhanced sensitivity is attributed to the surficial catalytic redox couple Fe(ii)/Fe(iii) induced by Fe of Fe@FeMnO. Besides, the catalytic kinetics was modelled and simulated, and the strong influence of the oxidation potential of the catalytic redox species on sensitivity was revealed. By tailoring the surficial atomic and electronic structures of the material, the redox potential can be altered, which can be used for controlling the electro-sensitivity and selectivity in electroanalysis.
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http://dx.doi.org/10.1039/d0cc07256gDOI Listing
February 2021

Analysis of Chemical Components in the Roots of Eight Intersubgeneric Hybrids of Paeonia.

Chem Biodivers 2021 Feb 3;18(2):e2000848. Epub 2021 Feb 3.

College of Landscape Architecture, Beijing Forestry University, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, Beijing, 100083, P. R. China.

Paeonia cultivars are famous ornamental plants, and some of them are also traditional Chinese medicinal resources. Intersubgeneric hybrids of Paeonia (IHPs) are formed by the hybridization of herbaceous peony (Paeonia lactiflora) and tree peony (Paeonia×suffruticosa or lutea hybrid tree peony). The phenotypic characteristics of IHPs are similar to those of herbaceous peony, and their root systems are large and vigorous. However, their medicinal value has not been reported yet. In this study, the roots of eight IHP samples were analyzed by high performance liquid chromatography quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF-MS/MS). A total of 18 compounds were identified, including phenols, paeonols, monoterpene glycosides, and tannins. The contents of monoterpene glycosides and tannins in IHPs were higher than herbaceous peony and tree peony, exceeding 44.76 mg/g DW and 11.50 mg/g DW, respectively. Three IHPs, 'Prairie Charm', 'Garden Treasure', and 'Yellow Emperor', with more types and a higher content of medicinal compounds, were screened out by cluster analysis. These IHPs have considerable potential for the development of medicinal resources.
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http://dx.doi.org/10.1002/cbdv.202000848DOI Listing
February 2021

Efficacy of different doses of dye-assisted internal limiting membrane peeling in idiopathic macular hole: a systematic review and network meta-analysis.

Int Ophthalmol 2021 Jan 3. Epub 2021 Jan 3.

Department of Ophthalmology, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China.

Purpose: Pars plana vitrectomy is the gold standard for the treatment of idiopathic macular hole. Several chromovitrectomy dyes have been used to improve the visualization of the internal limiting membrane (ILM), including indocyanine green, trypan blue (TB), brilliant blue G (BBG), and triamcinolone acetonide (TA). We conducted a network meta-analysis (NMA) to establish the optimum concentration of chromovitrectomy dye-assisted ILM peeling for IMH.

Methods: We searched PubMed, Embase, and Cochrane Library for relevant studies before January 2020. We performed a random-effects NMA using STATA version 15.1 to assess mean difference and odds ratios with 95% confidence intervals.

Results: We identified twelve retrospective trails and five randomized controlled trials (RCTs), comprising 1 492 patients of IMH on stage II-IV for ILM peeling. The results of IMH closure rate show that the effect of ILM peeling without dye was better than 0.25% ICG, the effects of ILM peeling with 0.5% ICG or TA were better than without dye, and the effects of ILM peeling with 0.05% BBG, 0.15% TB, 0.5% ICG or 0.05% ICG were better than 0.25% ICG. Ranking probability analysis shows that the rates of IMH closure after ILM peeling with 0.15% TB or 0.05% BBG were better than nine other concentrations of chromovitrectomy dyes.

Conclusion: The 0.15% TB and 0.05% BBG were recommended as the better efficient treatment-assisted ILM peeling for IMH closure. For retina specialists who prefer to use ICG to assist ILM peeling, 0.05% ICG may be a good choice. However, high-quality large-scale RCTs are recommended to confirm the NMA results.
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http://dx.doi.org/10.1007/s10792-020-01656-2DOI Listing
January 2021

A standardized pathological proposal for evaluating microvascular invasion of hepatocellular carcinoma: a multicenter study by LCPGC.

Hepatol Int 2020 Dec 28;14(6):1034-1047. Epub 2020 Dec 28.

Department of Pathology, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 225 Changhai Rd, Shanghai, 200438, China.

Background And Aims: Microvascular invasion (MVI) is a key pathological factor that severely affects the postoperative prognosis of patients with hepatocellular carcinoma (HCC). However, no MVI classification schemes based on standardized gross sampling protocols of HCC are available at present.

Methods: 119 HCC specimens were sampled at multiple sites (3-, 7-, and 13 points) for the optimum MVI detection rate. 16,144 resected HCCs were graded as M0, M1 or M2 by adopting three-tiered MVI grading (MVI-TTG) scheme based on the seven-point sampling protocol (SPSP). Survival analyses were performed on 2573 patients to explore the advantages of MVI-TTG.

Results: The MVI detection rate determined by SPSP was significantly higher than that determined by the 3-point sampling method (34.5% vs. 47.1%, p = 0.048), but was similar to that determined by the 13-point sampling method (47.1% vs. 51.3%, p = 0.517). Among 16,144 resected HCCs, the proportions of M0, M1 and M2 specimens according to SPSP were 53.4%, 26.2% and 20.4%, respectively. Postoperative survival analysis in 2573 HCC patients showed that the 3-year recurrence rates in M0, M1 and M2 MVI groups were 62.5%, 71.6% and 86.1%, respectively (p < 0.001), and the corresponding 3-year overall survival (OS) rates were 94.1%, 87.5% and 67.0%, respectively (p < 0.001). M1 grade was associated with early recurrence, while M2 grade was associated with both early and late recurrence. MVI-TTG had a larger area under the curve and net benefit rate than the two-tiered MVI grading scheme for predicting time to recurrence and OS.

Conclusions: SPSP is a practical method to balance the efficacy of sampling numbers and MVI detection rates. MVI-TTG based on SPSP is a better prognostic predictor than the two-tiered MVI scheme. The combined use of SPSP and MVI-TTG is recommended for the routine pathological diagnosis of HCC.
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http://dx.doi.org/10.1007/s12072-020-10111-4DOI Listing
December 2020

Blockade of spinal dopamine D1/D2 receptor suppresses activation of NMDA receptor through Gαq and Src kinase to attenuate chronic bone cancer pain.

J Adv Res 2021 Feb 13;28:139-148. Epub 2020 Aug 13.

Jiangsu Key Laboratory of TCM Evaluation and Translational Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu 211198, China.

Introduction: Spinal N-methyl-D-aspartate receptor (NMDAR) is vital in chronic pain, while NMDAR antagonists have severe side effects. NMDAR has been reported to be controlled by G protein coupled receptors (GPCRs), which might present new therapeutic targets to attenuate chronic pain. Dopamine receptors which belong to GPCRs have been reported could modulate the NMDA-mediated currents, while their exact effects on NMDAR in chronic bone cancer pain have not been elucidated.

Objectives: This study was aim to explore the effects and mechanisms of dopamine D1 receptor (D1DR) and D2 receptor (D2DR) on NMDAR in chronic bone cancer pain.

Methods: A model for bone cancer pain was established using intra-tibia bone cavity tumor cell implantation (TCI) of Walker 256 in rats. The nociception was assessed by Von Frey assay. A range of techniques including the fluorescent imaging plate reader, western blotting, and immunofluorescence were used to detect cell signaling pathways. Primary cultures of spinal neurons were used for in vitro evaluation.

Results: Both D1DR and D2DR antagonists decreased NMDA-induced upregulation of Ca oscillations in primary culture spinal neurons. Additionally, D1DR/D2DR antagonists inhibited spinal Calcitonin Gene-Related Peptide (CGRP) and c-Fos expression and alleviated bone cancer pain induced by TCI which could both be reversed by NMDA. And D1DR/D2DR antagonists decreased p-NR1, p-NR2B, and Gαq protein, p-Src expression. Both Gαq protein and Src inhibitors attenuated TCI-induced bone cancer pain, which also be reversed by NMDA. The Gαq protein inhibitor decreased p-Src expression. In addition, D1DR/D2DR antagonists, Src, and Gαq inhibitors inhibited spinal mitogen-activated protein kinase (MAPK) expression in TCI rats, which could be reversed by NMDA.

Conclusions: Spinal D1DR/D2DR inhibition eliminated NMDAR-mediated spinal neuron activation through Src kinase in a Gαq-protein-dependent manner to attenuate TCI-induced bone cancer pain, which might present a new therapeutic strategy for bone cancer pain.
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http://dx.doi.org/10.1016/j.jare.2020.08.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7753228PMC
February 2021

PTH1-34 inhibited TNF-α expression and antagonized TNF-α-induced MMP13 expression in MIO mice.

Int Immunopharmacol 2021 Feb 20;91:107191. Epub 2020 Dec 20.

Department of Rheumatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China. Electronic address:

This study aims to investigate the effects and mechanisms of parathyroid hormone [1-34] (PTH1-34) on TNF-α-stimulated mice chondrocytes, as well as cartilage from a meniscus injury induced osteoarthritis (MIO) mice model. The C57BL/6J mice received medial meniscectomy, and then administrated with PTH1-34. The results showed that PTH1-34 administration decreased secondary allodynia and the pain-related transcripts. The IHC, ELISA, Micro-CT imaging and histopathology analysis revealed the significantly improved subchondral plate thickness and bone porosity, the reduced pro-inflammatory cytokines in serum and joint fluid. In vitro, mice chondrocyte was treated with TNF-α or co-cultured with synovial cells. The results showed that TNF-α markedly upregulated the MMP13 expression, and the ERK1/2, NF-κB or PI3K signaling pathway inhibitors could reverse the induction effect of TNF-α on expression of MMP13 in chondrocytes. PTH1-34 alone has no effect on the expression of MMP13 and NF-κB signaling pathways, but the PTH1-34 could reverse the induction effect of TNF-α on MMP13 expression and NF-κB signaling pathway activation in chondrocytes. In addition, PTH1-34 administration inhibited the expression of TNF-α and MMP13, and chondrocyte viability, while the PKA repressor reversed the effect of PTH1-34 in chondrocytes co-cultured with synovial cells. In conclusion, PTH1-34 has an obvious analgesic and anti-inflammatory effect, inhibits the matrix synthesis and alleviates the progression of osteoarthritis. In vitro, PTH1-34 inhibited TNF-α expression and antagonized TNF-α-induced MMP13 expression via the PKA pathway and the NF-κB signaling pathways, respectively.
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http://dx.doi.org/10.1016/j.intimp.2020.107191DOI Listing
February 2021

Metabolomics Profiling and Diagnosis Biomarkers Searching for Drug-Induced Liver Injury Implicated to : A Cross-Sectional Cohort Study.

Front Med (Lausanne) 2020 30;7:592434. Epub 2020 Nov 30.

China Military Institute of Chinese Medicine, Fifth Medical Center of Chinese PLA General Hospital, Beijing, China.

The diagnosis of drug-induced liver injury (DILI) remains a challenge and the cases of Thunb. (PM) induced DILI (PM-DILI) have received much attention This study aimed to identify a simple and high-efficiency approach to PM-DILI diagnosis via metabolomics analysis. Plasma metabolites in 13 PM-DILI patients were profiled by liquid chromatography along with high-resolution mass spectrometry. Meanwhile, the metabolic characteristics of the PM-DILI were compared with that of autoimmune hepatitis (AIH), hepatitis B (HBV), and healthy volunteers. Twenty-four metabolites were identified to present significantly different levels in PM-DILI patients compared with HBV and AIH groups. These metabolites were enriched into glucose, amino acids, and sphingolipids metabolisms. Among these essential metabolites, the ratios of P-cresol sulfate vs. phenylalanine and inosine vs. bilirubin were further selected using a stepwise decision tree to construct a classification model in order to differentiate PM-DILI from HBV and AIH. The model was highly effective with sensitivity of 92.3% and specificity of 88.9%. This study presents an integrated view of the metabolic features of PM-DILI induced by herbal medicine, and the four-metabolite decision tree technique imparts a potent tool in clinical diagnosis.
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http://dx.doi.org/10.3389/fmed.2020.592434DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7734208PMC
November 2020

Cutaneous metastases of pancreatic carcinoma to the labia majora: A case report and review of literature.

World J Gastrointest Oncol 2020 Nov;12(11):1372-1380

Department of Dermatology, First Hospital of Jilin University, Changchun 130021, Jilin Province, China.

Background: Cutaneous metastases originating from pancreatic cancer are relatively rare. The most common reported site of metastasis is the umbilicus, and this manifestation is known as the Sister Mary Joseph's nodule. Non-umbilical cutaneous metastases are far less common, with only a few cases reported in the literature. Our case is the first case report, to our knowledge, on metastasis involving the labia majora and flat papules.

Case Summary: A 49-year-old Chinese female patient presented with a number of red, swollen papules on the vulva for 2 mo. Histological examination of the labia majora lesion revealed metastatic adenocarcinoma. The serum levels of tumor biomarkers CA199, CA242, and CA125 were significantly elevated. B-mode ultrasound-guided needle biopsy of the pancreas demonstrated moderately and poorly differentiated adenocarcinoma. The patient finally declined treatment for financial reasons and died 3 mo later.

Conclusion: Metastatic cutaneous lesions could indicate pancreatic cancer. Serum levels of tumor biomarkers may aid in diagnosing metastatic pancreatic adenocarcinoma.
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http://dx.doi.org/10.4251/wjgo.v12.i11.1372DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7667456PMC
November 2020

ATF4-modified serum exosomes derived from osteoarthritic mice inhibit osteoarthritis by inducing autophagy.

IUBMB Life 2021 Jan 28;73(1):146-158. Epub 2020 Nov 28.

Department of Rheumatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Activating transcription factor 4 (ATF4) is critical for chondrocyte proliferation and bone formation. Exosomes are considered as promising gene-delivery vehicles for the treatment of osteoarthritis (OA). This study utilized the serum-derived exosomes from OA mice as the gene-delivery vehicles for ATF4 gene therapy and explored their therapeutic effects on OA. Meniscus injury-induced OA model was established by the excision of anterior part of medial meniscus in the right knee of C57BL/6J mice. Exosomes were isolated from serum samples of sham and OA mice, and were referred to as sham-Exo and OA-Exo, respectively. ATF4-overexpressing OA-Exo (ATF4-OA-Exo) was developed by introducing ATF4 mRNA into OA-Exo via electroporation. Four weeks after surgery, OA mice received intra-articular injections of sham-Exo, OA-Exo, and ATF4-OA-Exo, respectively. The results showed that intra-articular injection of ATF4-OA-Exo alleviated articular cartilage degeneration or damage and inflammatory response of OA mice. Autophagy was weakened in knee joint cartilage of OA mice, which was partially restored by intra-articular injection of ATF4-OA-Exo. Further in vitro assays revealed that ATF4-OA-Exo promoted chondrocyte autophagy and inhibited chondrocyte apoptosis in the TNF-α- or tunicamycin-treated chondrocytes. Together, ATF4-modified serum exosomes derived from OA mice protect cartilage and alleviate OA progression by inducing autophagy.
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http://dx.doi.org/10.1002/iub.2414DOI Listing
January 2021

Notoginsenoside R1-Induced Neuronal Repair in Models of Alzheimer Disease Is Associated With an Alteration in Neuronal Hyperexcitability, Which Is Regulated by Nav.

Front Cell Neurosci 2020 4;14:280. Epub 2020 Sep 4.

Department of Neurosurgery, Shanghai Jiao Tong University Affiliated 6th People's Hospital, Shanghai, China.

Alzheimer disease is characterized by a progressive cognitive deficit and may be associated with an aberrant hyperexcitability of the neuronal network. Notoginsenoside R1 (R1), a major activity ingredient from , has demonstrated favorable changes in neuronal plasticity and induced neuroprotective effects in brain injuries, resulting from various disorders, however, the underlying mechanisms are still not well understood. In the present study, we aimed to explore the possible neuroprotective effects induced by R1 in a mouse model of AD and the mechanisms underlying these effects. Treatment with R1 significantly improved learning and memory functions and redressed neuronal hyperexcitability in amyloid precursor protein/presenilin-1 mice by altering the numbers and/or distribution of the members of voltage-gated sodium channels (Nav). Moreover, we determined whether R1 contributed to the regulation of neuronal excitability in Aβ-42-injured cells. Results of our study demonstrated that treatment with R1 rescued Aβ1-42-induced injured neurons by increasing cell viability. R1-induced alleviation in neuronal hyperexcitability might be associated with reduced Navβ2 cleavage, which partially reversed the abnormal distribution of Nav1.1α. These results suggested that R1 played a vital role in the recovery of Aβ1-42-induced neuronal injury and hyperexcitability, which is regulated by Nav proteins. Therefore, R1 may be a promising candidate in the treatment of AD.
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http://dx.doi.org/10.3389/fncel.2020.00280DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7500285PMC
September 2020

Secukinumab demonstrates high efficacy and a favorable safety profile over 52 weeks in Chinese patients with moderate to severe plaque psoriasis.

Chin Med J (Engl) 2020 Nov;133(22):2665-2673

Novartis Pharma AG, Basel 6463, Switzerland.

Background: Psoriasis is a chronic inflammatory skin disease, affecting about 0.6% of the Chinese population. Many patients are not well controlled by conventional treatments, thus there is need for new treatment regimens. In this study, we assessed the efficacy and safety of secukinumab in Chinese patients with moderate to severe plaque psoriasis.

Methods: This study was a 52-week, multicentre, randomized, double-blind, placebo-controlled, parallel-group, Phase 3 trial. A sub-population of study participants (≥18 years) of Chinese ethnicity were randomized to receive subcutaneous injections of 300 or 150 mg secukinumab, or placebo. The co-primary endpoints were psoriasis area severity index (PASI) 75 and Investigator's Global Assessment (IGA) 0/1 at Week 12.

Results: A total of 441 Chinese patients were enrolled in this study. Co-primary outcomes were achieved; 300 and 150 mg secukinumab were superior to placebo as shown in the proportion of patients that achieved PASI 75 (97.7% and 87.2% vs. 3.7%, respectively; P < 0.001), and IGA 0/1 (82.3% and 69.7% vs. 2.7%; P < 0.001) at Week 12. Treatment efficacy was maintained until Week 52. There was no increase in overall adverse events with secukinumab relative to placebo throughout the 52-week period.

Conclusion: Secukinumab is highly effective and well tolerated in Chinese patients with moderate to severe plaque psoriasis.

Trial Registration: ClinicalTrials.gov, NCT03066609; https://clinicaltrials.gov/ct2/show/record/NCT03066609.
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http://dx.doi.org/10.1097/CM9.0000000000001163DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7647502PMC
November 2020

[Research advances in the mechanism of acupuncture in regulating tumor immunosuppression].

Zhen Ci Yan Jiu 2020 Sep;45(9):767-70

Acupuncture Research Center, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China; College of Acupuncture and Massage, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.

As a characteristic therapy in traditional Chinese medicine, acupuncture has shown potential advantages in anti-tumor therapy, and one of the therapeutic effects of acupuncture is to improve the immunosuppressive conditions in patients with tumor. Based on the immunoregulatory effect of acupuncture, this article summarized the mechanism of acupuncture in regulating tumor immune status from the following aspects: stimulating the activation of natural killer cells, increasing the number of CD8+ T cells, and adjusting the balance between T helper 1 cells and T helper 2 cells and between regulatory T cells and T helper 17 cells. With reference to existing evidence, we believe that acupuncture can regulate the body's immunosuppressive conditions through a variety of targets, but further clinical and basic studies are needed to clarify its regulatory effect on tumor immune microenvironment and related mechanism of action.
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http://dx.doi.org/10.13702/j.1000-0607.190955DOI Listing
September 2020

Pressure-Induced Modulation of Electronic and Optical Properties of Surface O-Functionalized TiC MXene.

ACS Omega 2020 Sep 25;5(35):22248-22254. Epub 2020 Aug 25.

College of Physics and Engineering, Henan University of Science and Technology, Luoyang 471023, China.

Functionalized MXenes have gained increasing interest in the fields of thermoelectric materials, hydrogen storage, and so forth. In this work, pressure-induced band modulation and optical properties of the TiCO monolayer are investigated by using density functional theory with the hybrid (HSE06) functional. The calculation reveals that TiCO MXenes under pressure are stable because of the positive . TiCO undergoes a semiconductor-to-metal phase transition at about 7 GPa. The metallization of TiCO mainly results from the Ti-d state. Research indicates that there exist strong interactions between Ti-d and C-p, and Ti-d and O-p states, which are further confirmed by the charge analysis. In addition, the absorption is enhanced in the visible region with increasing pressure. We also observed some new absorption peaks in the visible region.
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http://dx.doi.org/10.1021/acsomega.0c02435DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7482309PMC
September 2020

The pathogenesis and treatment in antineutrophil cytoplasmic antibody associated vasculitis.

Am J Transl Res 2020 15;12(8):4094-4107. Epub 2020 Aug 15.

Department of Rheumatology, The First Affiliated Hospital of Zhengzhou University Zhengzhou, China.

Anti-neutrophil cytoplasmic antibody (ANCA) associated vasculitis (AAV) is a systemic autoimmune disease characterized by leukocytoclastic inflammation of small blood vessels. Commonly detected autoantibodies include anti-protease 3 (PR3) and anti-myeloperoxidase (MPO). Although cell necrosis plays an important role in the production of autoantibodies and the pathogenesis of AAV, the correlation between their titers and disease activity remains elusive. As improved detection techniques facilitate early diagnosis, a satisfactory efficacy can be achieved in patients with mild to medium severe AAV treated with glucocorticoids and immunosuppressants. However, resistant and relapsing AAV, sometimes life-threatening, do exist in clinical practice. In-depth understanding of pathogenesis of AAV may lend novel insight into the mechanism responsible for its formation and help find effective targeted therapies for refractory patients.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7476117PMC
August 2020

[Progress of research on acupuncture treatment of nonspecific low back pain].

Zhen Ci Yan Jiu 2020 Aug;45(8):682-6

Acupuncture Research Center, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.

Nonspecific low back pain (NLBP) is a heterogeneous disease with no definite pathological anatomical causes. Although acupuncture treatment of NLBP has been included in the international clinical guidelines for low back pain in many countries, the grade strength is still low, and the Results of clinical efficacy evaluation are controversial. Moderate evidence from international studies indicates that acupuncture treatment of NLBP has a clear short-term analgesic effect and can improve pain related depression symptoms to a certain extent, but the exploration of efficacy rules is relatively insufficient, and there is no specific operational scheme to guide the acupuncture treatment of NLBP. Domestic clinical studies are quite distinctive in the aspects of dose-effect acupoint selection, characteristic needling Methods, and acupuncture apparatuses, but lack of systematic research, and have low quality and weak evidence. In terms of the underlying mechanisms, acupuncture may achieve analgesic effect through anti-inflammation, relieving central sensitization, and improving the damage of local multifidus muscles and bones. However, due to the heterogeneity of NLBP disease, the current elaboration on the mechanism of acupuncture treatment of NLBP may not reach a consensus, and it is still worth further exploration.
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http://dx.doi.org/10.13702/j.1000-0607.190877DOI Listing
August 2020

Carboxymethyl cellulose-based cryogels for efficient heavy metal capture: Aluminum-mediated assembly process and sorption mechanism.

Int J Biol Macromol 2020 Dec 25;164:3275-3286. Epub 2020 Aug 25.

School of Light Industry and Chemical Engineering, Dalian Polytechnic University, Dalian 116034, China. Electronic address:

Heavy metal ions pollution is a terrible issue that needs to be efficiently treated as a matter of priority to construct our sustainable society. However, the easy-to-handling of high-performance biomass-derived sorbents with fascinating features like high sorption capacity, favorable separation and recycling remain challenging. Herein, the development of a novel bead-like adsorbent with above features, that is, Al(III)-assembled carboxymethyl cellulose beads were used for the removal of Pb(II), Ni(II) and Co(II) from aqueous solution. Characterization methods like FT-IR, SEM, XPS and TGA were employed to confirm its physicochemical properties. Removal of the three heavy metal ions at different pH values, initial concentration and contact time were discussed at batch adsorption experiments. Meanwhile, regeneration was also discussed deeply. The results revealed that the adsorption capacity of the sorbents for three heavy metals increases with increasing pH and the initial concentration. The adsorption isotherm could be described well by the Freundlich model, and the maximum adsorption capacity for Pb(II), Ni(II) and Co(II) were 550, 620 and 760 mg/g, respectively. Kinetics study indicated that the Pseudo-second-order model described the best correlation with experimental data, this suggested that the complexation may participated in the adsorption process. More significantly, this type of bead-like adsorbents displayed excellent reusability after four sequential cycles.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.08.186DOI Listing
December 2020

Measurements of the parapapillary atrophy area and other fundus morphological features in high myopia with or without posterior staphyloma and myopic traction maculopathy.

Int J Ophthalmol 2020 18;13(8):1272-1280. Epub 2020 Aug 18.

Department of Ophthalmology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China.

Aim: To investigate the affecting factors of parapapillary gamma and delta zones and other fundus morphological features in high myopia.

Methods: Seventy high myopia patients were included in this retrospective observational study and 47 patients were female. Patients were divided into three groups: no posterior staphyloma (no PS), PS with myopic traction maculopathy (PS with MTM), and PS without MTM using 3-dimensional magnetic resonance imaging and optical coherence tomography. MTM patients were further classified into three types [epiretinal membrane, macular hole, and macular retinoschisis (MRS)]. Diameters of the gamma and delta zones were measured among other morphometric variables using fundus photographs.

Results: Of the 70 individuals (127 eyes), the mean age was 57.46±13.56y. In univariate analysis, morphological features changed most dramatically in PS with MTM patients, who had the largest gamma zone diameters, the largest disk-fovea distance (DFD) and disk-fovea angle, and the smallest angle kappa and vertical distance of temporal arterial arcade. However, their horizontal delta zone diameter was smaller than in the patients with PS yet without MTM. In multivariate analysis, with axial length (AL) and age adjusted, the horizontal diameter in the delta zone of the PS without MTM group was still significantly larger than in the PS with MTM group (=0.024). Comparing the three subtypes of MTM patients, the diameters of the gamma zone and DFD in MRS group were the largest.

Conclusion: The characteristics of the gamma and delta zones change inconsistently in different stages of high myopia. These changes may be associated with anatomical changes caused by local traction. Factors such as PS, AL and age play an important role. These findings may provide a hint about the pathogenesis of traction in high myopia.
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http://dx.doi.org/10.18240/ijo.2020.08.14DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7387912PMC
August 2020

Identification of key genes for type 1 diabetes mellitus by network-based guilt by association.

Rev Assoc Med Bras (1992) 2020 Jun 20;66(6):778-783. Epub 2020 Jul 20.

Department of Internal Medicine, Linyi Luozhuang Central Hospital, Linyi, China.

OBJECTIVE This study aimed to propose a co-expression-network (CEN) based gene functional inference by extending the "Guilt by Association" (GBA) principle to predict candidate gene functions for type 1 diabetes mellitus (T1DM). METHODS Firstly, transcriptome data of T1DM were retrieved from the genomics data repository for differentially expressed gene (DEGs) analysis, and a weighted differential CEN was generated. The area under the receiver operating characteristics curve (AUC) was chosen to determine the performance metric for each Gene Ontology (GO) term. Differential expression analysis identified 325 DEGs in T1DM, and co-expression analysis generated a differential CEN of edge weight > 0.8. RESULTS A total of 282 GO annotations with DEGs > 20 remained for functional inference. By calculating the multifunctionality score of genes, gene function inference was performed to identify the optimal gene functions for T1DM based on the optimal ranking gene list. Considering an AUC > 0.7, six optimal gene functions for T1DM were identified, such as regulation of immune system process and receptor activity. CONCLUSIONS CEN-based gene functional inference by extending the GBA principle predicted 6 optimal gene functions for T1DM. The results may be potential paths for therapeutic or preventive treatments of T1DM.
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http://dx.doi.org/10.1590/1806-9282.66.6.778DOI Listing
June 2020

The blood protective effect of autologous platelet separation in aortic dissection.

Clin Hemorheol Microcirc 2020 ;76(3):361-366

Department of Blood Transfusion, Qilu Hospital of Shandong University, Jinan, Shandong, China.

Objective: This study aimed to retrospectively analyze the blood protective effect of autologous platelet separation in operations for acute aortic dissection.

Methods: A total of 130 patients with acute aortic dissection were enrolled into the present study. The average age of these patients was 52.962±10.5061 years old. These patients underwent the modified aortic arch replacement with the elephant trunk technique or endovascular aortic exclusion with covered stent. Among these patients, 68 patients who underwent autologous platelet separation were assigned to the platelet separation group, while the remaining patients were assigned to the control group. All operations were performed under deep hypothermic circulatory arrest. After anesthesia, 1-2 therapeutic doses of autologous platelets were isolated from patients in the platelet separation group, and these platelets were quickly infused back to these patients after heparin was neutralized by protamine at the end of the cardiopulmonary bypass. The preoperative and postoperative indexes in these two groups were compared.

Results: There were no statistically significant differences in age, gender, smoking history, drinking history and hypertension history between these two groups. Compared with controls, the transfusion volume of allogeneic platelets in the perioperative period significantly decreased in the platelet separation group (1.919±1.6226 vs. 0.794±1.1789, P < #x003C;< #x200A;0.05), and the use rate of allogeneic platelets also significantly decreased (74.19% vs. 45.59%, P < #x003C;< #x200A;0.05).

Conclusion: The intraoperative auto transfusion of platelets significantly reduced the volume of allogeneic platelet transfusion after the operation for aortic dissection, which has a significant blood protective effect.
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http://dx.doi.org/10.3233/CH-200871DOI Listing
February 2021

Effects of lifestyle interventions on rural patients with type 2 diabetes mellitus.

World J Diabetes 2020 Jun;11(6):261-268

Department of Osteoarthropathy, Yantaishan Hospital, Yantai 264025, Shandong Province, China.

Background: The prevalence of type 2 diabetes mellitus (T2DM) is rising rapidly in rural areas, and lifestyle interventions can effectively reduce the blood glucose levels of patients with T2DM. However, current dietary and exercise guidelines are still at experimental stages and are difficult for subjects to understand and implement. The Human Metabolism Analyzer provides real life interventions for the prevention and treatment of T2DM, and our pilot research has demonstrated its effectiveness and good compliance.

Aim: To investigate the effect of and compliance with lifestyle interventions in rural patients with T2DM.

Methods: A total of ten rural villages were randomly selected in Chaoshui Township, Penglai City, Shandong Province, China, to conduct health screening among residents aged 50 years or older. Each rural village represented a group, and 12 patients with T2DM were randomly selected from each group (total: 120) to participate in this study and receive real life lifestyle interventions and medication guidance. Lifestyle interventions included changing the meal order (A), postprandial activities (B), resistance exercise (C), and reverse abdominal breathing (D). Diabetes education was conducted at least once a month with a weekly phone follow-up to monitor exercise and diet. Waist circumference, blood pressure, body mass index (BMI), motor function, body composition, fasting blood glucose, and glycated hemoglobin (HbA1c) were analyzed before and 3 mo after the intervention. Moreover, patient compliance and adjustments of hypoglycemic drugs were evaluated.

Results: A total of 109 subjects completed the study. The compliance rates for lifestyle interventions A, B, C, and D were 57.79%, 60.55%, 64.22%, and 75.23%, respectively. Among the subjects who received hypoglycemic drugs, the dose was reduced 2 to 3 times based on blood glucose in 54 (67.50%) subjects and was tapered and discontinued in 5 (6.25%) subjects within 3 mo, with no significant fluctuations in blood glucose after dose reduction and withdrawal. After lifestyle interventions, waist circumference, BMI, fasting blood glucose, and HbA1c significantly decreased ( 0.001); motor function and body composition also significantly improved ( 0.001).

Conclusion: For patients with T2DM, compliance to real-life lifestyle interventions is good, and the interventions significantly improve metabolic indicators such as waist circumference, BMI, blood pressure, HbA1c, body composition, and motor function. Some patients are able to taper or discontinue hypoglycemic drugs.
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http://dx.doi.org/10.4239/wjd.v11.i6.261DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7284015PMC
June 2020