Publications by authors named "Shan Jin"

243 Publications

Identification and function analysis of an immune deficiency homolog in swimming crab, Portunus trituberculatus.

Fish Shellfish Immunol 2022 Jan 11;121:245-253. Epub 2022 Jan 11.

Key Laboratory of the Ministry of Education for Applied Marine Biotechnology, School of Marine Science, Ningbo University, Ningbo, 315211, China. Electronic address:

The immune deficiency (IMD) pathway is involved in both antiviral and antibacterial immune responses in Drosophila. IMD protein is the key adaptor to link the extracellular signal and the intracellular reaction to initiate the signal transduction in IMD pathway. In present study, the cDNA of the IMD (Pt-IMD) was identified from a marine crab, Portunus trituberculatus. The Pt-IMD is predicted to encode 170 amino acids with a death domain. Real-Time quantitative PCR analysis showed that Pt-IMD was constitutively expressed in hemocytes, intestine, gill, heart, muscle and hepatopancreas in normal crab. Moreover, the transcript of Pt-IMD in large-granule hemocytes is approximately 6-fold higher than semi-granular cells and agranular cells. Intracellular localization showed Pt-IMD was distributed mainly in the cytoplasm when it was over-expressed in Drosophila Schneider 2 (S2) cell. Functionally, over-expression of Pt-IMD could activate the promoters of Drosophila antimicrobial peptide genes (AMPs) in S2 cell. Furthermore, Pt-IMD expression was also knock-down by RNAi to determine the function of Pt-IMD on regulation of the expression of different antimicrobial peptides (AMPs) in crab. In the primary cultured hemocytes challenged with or without Vibrio alginolyticus, after Pt-IMD was knocked-down by specific long double strand RNA, the expression of anti-lipopolysaccharide factor1 (ALF1), ALF3, crustin1, crustin3, arasin2, hyastatin1and hyastatin3 have been significantly inhibited in normal cell or bacterial infected cell, while the expression of lysozyme was normal in non-infected cells and was significantly induced in bacterial infected cells, which compared to the non-specific dsRNA treated cells.
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http://dx.doi.org/10.1016/j.fsi.2022.01.014DOI Listing
January 2022

Cloning, Functional Characterization and Response to Cadmium Stress of the Thioredoxin-like Protein 1 Gene from .

Int J Mol Sci 2021 Dec 29;23(1). Epub 2021 Dec 29.

Key Laboratory of Applied Marine Biotechnology by the Ministry of Education, Ningbo University, Ningbo 315012, China.

Cadmium (Cd) is a heavy metal toxicant and is widely distributed in aquatic environments. It can cause excessive production of reactive oxygen species (ROS) in the organism, which in turn leads to a series of oxidative damages. Thioredoxin (Trx), a highly conserved disulfide reductase, plays an important role in maintaining the intracellular redox homeostasis in eukaryotes and prokaryotes. is an edible marine worm, an invertebrate that is extensively found on the mudflats of coastal China. To explore the molecular response of in mudflat organisms under Cd stress, we identified a new isoform (Trx-like protein 1 gene) from for the first time, designated as . Molecular and structural characterization, as well as multiple sequence and phylogenetic tree analysis, demonstrated that Trxl belongs to the Trx superfamily. transcripts were found to be ubiquitous in all tissues, and the highest expression level occurred in the coelomic fluid. Exposure to three sublethal concentrations of Cd resulted in the upregulation and then downregulation of expression levels over time in coelomic fluid of . The significant elevation of expression after 12 and 24 h of Cd exposure at 6 and 96 mg/L, respectively, might reflect its important role in the resistance to Cd stress. Recombinant Trxl (Trxl) showed prominent dose-dependent insulin-reducing and ABTS free radical-scavenging abilities. After exposure to 96 mg/L Cd for 24 h, the ROS level increased significantly in the coelomic fluid, suggesting that Cd induced oxidative stress in . Furthermore, the injection of Trxl during Cd exposure significantly reduced the ROS in the coelomic fluid. Our data suggest that Trxl has significant antioxidant capacity and can protect from Cd-induced oxidative stress.
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http://dx.doi.org/10.3390/ijms23010332DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8745482PMC
December 2021

Kidney Failure Risk Equation and Cost of Care in Patients with Chronic Kidney Disease.

Clin J Am Soc Nephrol 2022 Jan 30;17(1):17-26. Epub 2021 Dec 30.

Section of Nephrology, Kidney Health Centre, Saskatchewan, Canada.

Background And Objectives: Patients with CKD exhibit heterogeneity in their rates of progression to kidney failure. The kidney failure risk equation (KFRE) has been shown to accurately estimate progression to kidney failure in adults with CKD. Our objective was to determine health care utilization patterns of patients on the basis of their risk of progression.

Design, Setting, Participants, & Measurements: We conducted a retrospective cohort study of adults with CKD and eGFR of 15-59 ml/min per 1.73 m enrolled in multidisciplinary CKD clinics in the province of Saskatchewan, Canada. Data were collected from January 1, 2004 to December 31, 2012 and followed for 5 years (December 31, 2017). We stratified patients by eGFR and risk of progression and compared the number and cost of hospital admissions, physician visits, and prescription drugs.

Results: In total, 1003 adults were included in the study. Within the eGFR of 15-29 ml/min per 1.73 m group, the costs of hospital admissions, physician visits, and drug dispensations over the 5-year study period comparing high-risk patients with low-risk patients were (Canadian dollars) $89,265 versus $48,374 (=0.008), $23,423 versus $11,231 (<0.001), and $21,853 versus $16,757 (=0.01), respectively. Within the eGFR of 30-59 ml/min per 1.73 m group, the costs of hospital admissions, physician visits, and prescription drugs were $55,944 versus $36,740 (=0.10), $13,414 versus $10,370 (=0.08), and $20,394 versus $14,902 (=0.02) in high-risk patients in comparison with low-risk patients, respectively, for progression to kidney failure.

Conclusions: In patients with CKD and eGFR of 15-59 ml/min per 1.73 m followed in multidisciplinary clinics, the costs of hospital admissions, physician visits, and drugs were higher for patients at higher risk of progression to kidney failure by the KFRE compared with patients in the low-risk category. The high-risk group of patients with CKD and eGFR of 15-29 ml/min per 1.73 m had stronger association with hospitalizations costs, physician visits, and drug utilizations.
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http://dx.doi.org/10.2215/CJN.06770521DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8763151PMC
January 2022

Lipidomics analysis unravels changes from flavor precursors in different processing treatments of purple-leaf tea.

J Sci Food Agric 2021 Dec 17. Epub 2021 Dec 17.

College of Horticulture, Fujian Agriculture and Forestry University/Key Laboratory of Tea Science in University of Fujian Province, Fuzhou, China.

Background: Lipids are one of the most important bioactive compounds, affecting the character and quality of tea. However, the contribution of lipids to tea productions is still elusive. Here, we systematically identified the lipid profiles of green, oolong, and black teas in purple-leaf tea (Jinmingzao, JMZ) and green-leaf tea (Huangdan, HD), respectively.

Results: The lipids analysis showed regular accumulation in tea products with different manufacturing processes, among which the fatty acids, glycerolipids, glycerophospholipids, and sphingolipids contribute to the quality characteristics of tea products, including typical fatty acyl (FA), monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerols (DGDG), and phosphatidylcholine (PC). Compared tea materials with products, levels of fatty acids were up-regulated, while glycerolipids and glycerophospholipids were down-regulated in tea products. FA 18:3, FA 16:0, MGDG 36:6, DGDG 36:6, PC 34:3, and PC 36:6 were the negative contributors to green tea flavor formation of purple-leaf tea. The pathway analysis of significant lipids in materials and products of purple-leaf tea were enriched linolenic acid metabolism pathway and glycerolipid metabolism.

Conclusion: This study provides insights into the lipid metabolism profiles of different tea leaf colors, and found that fatty acids are essential precursors of black tea flavor formation.
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http://dx.doi.org/10.1002/jsfa.11721DOI Listing
December 2021

[Differential proteins in the prostate fluid with abnormal lecithin bodies and immunologic mechanism of chronic prostatitis patients].

Zhonghua Nan Ke Xue 2021 Jul;27(7):609-615

Department of Urology, The First Affiliated Hospital of Tianjin University of Chinese Medicine, Tianjin 300192, China.

Objective: To study the protein changes in the prostate fluid with abnormal lecithin bodies, and explore their relationship with the pathogenesis of chronic prostatitis.

Methods: Using the tandem mass tag (TMT) technology, we identified differential proteins in the prostate fluid with abnormal lecithin bodies in normal males and analyzed their biological functions by GO functional annotation and KEGG pathways.

Results: Totally, 377 differential proteins were identified, 238 up-regulated and the other 139 down-regulated, and 8 of the top 20 significant differential proteins were related to inflammation and immune regulation. In the top 30 of the 1 011 items enriched by GO annotation, 5 were related to immune function and 3 to inflammatory response, and of the 251 KEGG pathways, 9 were involved in inflammation and immune regulation.

Conclusions: Changes in lecithin bodies lead to proteomic changes in the prostatic fluid, which is closely related to protein- and pathway-associated inflammatory response and immune disorders, as well as to the immunologic mechanism of chronic prostatitis patients, and therefore can be used as an indicator of chronic prostatitis.
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July 2021

Specificity protein 1/microRNA-92b forms a feedback loop promoting the migration and invasion of head and neck squamous cell carcinoma.

Bioengineered 2021 12;12(2):11397-11409

Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, China Medical University, Liaoning Provincial Key Laboratory of Oral Diseases, No.117, Nanjing Bei Street, Heping District, Shenyang, Liaoning, 110002, People's Republic of China.

In this study we report a novel specificity protein 1 (SP1)/microRNA-92b (miR-92b) feedback loop regulating the migration and invasion of head and neck squamous cell carcinoma (HNSCC). Microarray and real-time Polymerase Chain Reaction (PCR) were used to detect gene expression in HNSCC tissues and cell lines. Transwell migration, invasion, wound healing and cell counting kit - 8 (CCK-8) cell assays were used to compare cell migration, invasion and proliferation abilities. Chromatin Immunoprecipitation (ChIP) assays were used to detect SP1 binding to the miR-92b promoter. Western blot was used to detect protein levels. An in vivo tumorigenesis experiment was used to evaluate the effect of SP1 knockdown on tumor growth and protein levels were evaluated by immunohistochemistry. We found that the miR-92b expression level was elevated in HNSCC primary focus tissue compared with adjacent normal tissue, and a higher level of miR-92b was related to a higher clinical stage and worse prognosis of HNSCC patients. MiR-92b and SP1 mutually promoted each expression and cooperatively facilitated the migration, invasion and proliferation of HNSCC cells. A decreased level of SP1/miR-92b resulted in a restraint of in vivo tumor growth. In conclusion, our results suggest that the SP1/miR-92b feedback loop generally promotes HNSCC invasion and metastasis, thus presenting a possible therapeutic target in the treatment of HNSCC patients.
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http://dx.doi.org/10.1080/21655979.2021.2008698DOI Listing
December 2021

Highlights of light meson spectroscopy at the BESIII experiment.

Natl Sci Rev 2021 Nov 2;8(11):nwab198. Epub 2021 Nov 2.

Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China.

Hadron spectroscopy provides a way to understand the dynamics of the strong interaction. For light hadron systems, only phenomenological models or lattice quantum chromodynamics (QCD) are applicable, because of the failure of perturbation expansions for QCD at low energy. Experimental data on light hadron spectroscopy are therefore crucial to provide necessary constraints on various theoretical models. Light meson spectroscopy has been studied using charmonium decays with the Beijing Spectrometer Experiment (BES) at the Beijing Electron-Positron Collider, operating at 2.0-4.6 GeV center-of-mass energy, for nearly three decades. Charmonium data with unprecedented statistics and well-defined initial and final states provide BESIII with unique opportunities to search for glueballs, hybrids and multi-quark states, as well as perform systematic studies of the properties of conventional light mesons. In this article, we review BESIII results that address these issues.
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http://dx.doi.org/10.1093/nsr/nwab198DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8645028PMC
November 2021

Impact of comorbid post traumatic stress disorder on multiple sclerosis in military veterans: A population-based cohort study.

Mult Scler 2021 Dec 2:13524585211058361. Epub 2021 Dec 2.

Multiple Sclerosis Center of Excellence, Veterans Affairs, and Department of Neurology, University of Maryland School of Medicine, Baltimore, MD, USA.

Background: Very little is known regarding the impact of post traumatic stress disorder (PTSD) on the course of multiple sclerosis (MS).

Objectives: To explore the impact of pre-existing PTSD on MS relapses, magnetic resonance imaging (MRI) activity, and disability in a large population-based cohort.

Methods: Military Veterans with MS and PTSD prior to symptom onset (MS,  = 96) were identified using the Department of Veterans Affairs MS databases. MS cases were matched to MS controls without PTSD ( = 95). Number of relapses, number of new T2 lesions and new gadolinium lesions on brain MRI, and neurological disability were abstracted between 2015 and 2019.

Results: The mean annualized relapse rate was greater in the MS group versus controls (0.23 vs 0.06, respectively;  < 0.05), as was the annualized mean number of new T2 and gadolinium-enhancing lesions on brain MRI (0.52 vs 0.16 and 0.29 vs 0.08, respectively;  < 0.05). Disability accrual (time to Disability Status Scale 6.0) was more rapid (23.7 vs 29.5 years,  < 0.05) in relapsing MS patients with PTSD.

Conclusion: Patients with MS have higher disease activity and reach disability endpoints more rapidly than controls. This is the first study to show PTSD as a potentially modifiable risk factor for MS relapses, MRI activity, and disability.
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http://dx.doi.org/10.1177/13524585211058361DOI Listing
December 2021

Long non-coding RNA MIR31HG as a prognostic predictor for malignant cancers: A meta- and bioinformatics analysis.

J Clin Lab Anal 2022 Jan 27;36(1):e24082. Epub 2021 Nov 27.

NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases (First Affiliated Hospital, School of Medicine, Shihezi University), Shihezi, China.

Background: The possible regulatory mechanism of MIR31HG in human cancers remains unclear, and reported results of the prognostic significance of MIR31HG expression are inconsistent.

Methods: The meta-analysis and related bioinformatics analysis were conducted to evaluate the role of MIR31HG in tumor progression.

Results: The result showed that high MIR31HG expression was not related to prognosis. However, in the stratified analysis, we found that the overexpression of MIR31HG resulted in worse OS, advanced TNM stage, and tumor differentiation in respiratory system cancers. Moreover, our results also found that MIR31HG overexpression was related to shorter OS in cervical cancer patients and head and neck tumors. In contrast, the MIR31HG was lower in digestive system tumors which contributed to shorter overall survival, advanced TNM stage, and distant metastasis. Furthermore, the bioinformatics analysis showed that MIR31HG was highly expressed in normal urinary bladder, small intestine, esophagus, stomach, and duodenum and low in colon, lung, and ovary. The results obtained from FireBrowse indicated that MIR31HG was highly expressed in LUSC, CESC, HNSC, and LUAD and low in STAD and BLCA. Gene Ontology analysis showed that the co-expressed genes of MIR31HG were most enriched in the biological processes of peptide metabolism and KEGG pathways were most enriched in Ras, Rap1, and PI3K-Akt signaling pathway.

Conclusion: MIR31HG may serve as a potential biomarker in human cancers.
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http://dx.doi.org/10.1002/jcla.24082DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8761471PMC
January 2022

Angle-resolved polarized Raman spectra of the basal and edge plane of MoS.

Opt Express 2021 Oct;29(21):32818-32825

Angle-resolved polarized (ARP) Raman spectroscopy can be utilized to characterize the Raman modes of two-dimensional layered materials based on crystal symmetry or crystal orientation. In this paper, the polarization properties of and A modes on the basal plane and edge plane of high purity 2H-MoS bulk crystal grown by chemical vapor transport (CVT) method were investigated by ARP Raman spectroscopy. The I and II type ARP Raman spectroscopy with four kinds of polarization configurations: αY, αX, βY, and βX were used to explore the intensity dependence of and A modes at different planes on the polarization direction of incident/scattered light. The results show that the and A modes exhibit different polarization properties dependent on the polarization of the incident laser and the in-plane rotation of the sample at different planes. The experimental results were confirmed and analyzed through theoretical calculation. Our work sheds light on the intriguing effect of the subtle atomic structure in stacked MoS layers on the resulting ARP Raman properties. This provides a reference for the study of other two-dimensional layered crystalline materials by ARP Raman spectroscopy.
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http://dx.doi.org/10.1364/OE.435835DOI Listing
October 2021

Substituent-mediated quantum interference toward a giant single-molecule conductance variation.

Nanotechnology 2021 Dec 9;33(9). Epub 2021 Dec 9.

Key Laboratory of the Ministry of Education for Advanced Catalysis Materials, Institute of Physical Chemistry, Zhejiang Normal University, Jinhua 321004, People's Republic of China.

Quantum interference (QI) in single molecular junctions shows a promising perspective for realizing conceptual nanoelectronics. However, controlling and modulating the QI remains a big challenge. Herein, two-type substituents at different positions of-linked benzene, namely electron-donating methoxy (-OMe) and electron-withdrawing nitryl (-NO), are designed and synthesized to investigate the substituent effects on QI. The calculated transmission coefficients() indicates that -OMe and -NOcould remove the antiresonance and destructive quantum interference (DQI)-induced transmission dips at position 2. -OMe could raise the antiresonance energy at position 4 while -NOgroups removes the DQI features. For substituents at position 5, both of them are nonactive for tuning QI. The conductance measurements by scanning tunneling microscopy break junction show a good agreement with the theoretical prediction. More than two order of magnitude single-molecule conductance on/off ratio could be achieved at the different positions of -NOsubstituent groups at room temperature. The present work proves chemical substituents can be used for tuning QI features in single molecular junctions, which provides a feasible way toward realization of high-performance molecular devices.
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http://dx.doi.org/10.1088/1361-6528/ac3b84DOI Listing
December 2021

Mechanically Induced Switching between Two Discrete Conductance States: A Potential Single-Molecule Variable Resistor.

ACS Appl Mater Interfaces 2021 Dec 19;13(48):57646-57653. Epub 2021 Nov 19.

Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, China.

The fabrication of solid-state single-molecule switches with high on-off conductance ratios has been proposed to advance conventional technology in areas such as molecular electronics. Herein, we employed the scanning tunneling microscope break junction (STM-BJ) technique to modulate conductance in single-molecule junctions using mechanically induced stretching. Compound possesses two dihydrobenzothiophene (DHBT) anchoring groups at the opposite ends linked with rigid alkyne side arms to form a gold-molecule-gold junction, while contains 4-pyridine-anchoring groups. The incorporation of ferrocene into the backbone of each compound allows rotational freedom to the cyclopentadienyl (Cp) rings to give two distinct conductance states (high and low) for each. Various control experiments and suspended junction compression/retraction measurements indicate that these high- and low-conductance plateaus are the results of conformational changes within the junctions (extended and folded states) brought about by mechanically induced stretching. A high-low switching factor of 42 was achieved for , whereas an exceptional conductance ratio in excess of 2 orders of magnitude (205) was observed for . To the best of our knowledge, this is the highest experimental on-off conductance switching ratio for a single-molecule junction exploiting the mechanically induced STM-BJ method. Computational studies indicated that the two disparate conductance states observed for and result from mechanically induced conformational changes due to an interplay between conductance and the dihedral angles associated with the electrode-molecule interfaces. Our study reveals the structure-function relationship that determines conductance in such flexible and dynamic systems and promotes the development of a single-molecule variable resistor with high on-off switching factors.
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http://dx.doi.org/10.1021/acsami.1c12151DOI Listing
December 2021

Identifying barriers to sustainable apple production: A stakeholder perspective.

J Environ Manage 2022 Jan 11;302(Pt B):114082. Epub 2021 Nov 11.

School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK. Electronic address:

Apple is one of the most important cash crops in China. However, negative economic, environmental and social impacts are associated with its production. This study aims to apply a holistic systems perspective to understand existing problems associated with apple production in China and use this information to improve its sustainability. A structured survey was administered to farmers (n = 245) in Shandong and Shanxi provinces, combined with semi-structured interviews with apple supply chain stakeholders (n = 25). Themes, dimensions and relationships were identified based on an inductive thematic analysis of interview data, and then triangulated against the survey data. Interpretive Structural Modelling and Cross-Impact Matrix Multiplication Applied to Classification methods were applied to investigate interrelationships and effects of the elicited elements within the system. The results indicated that various environmental, economic and social problems are associated with apple production in China, including environmental and health risks associated with synthetic input applications, yield instability, deterioration of apple quality, farmers' uncertainty about accessing routes to market, and the ageing farming workforce. The interaction of socio-economic and supply chain issues has contributed to the system "lock-in" to unsustainable practices within the apple production system. Existing agricultural policies were ineffective as they did not include policy leverage to mitigate the multiple factors driving lock-in to unsustainable practices within the system. The research has provided evidence to enable policymakers to develop effective and targeted strategies to facilitate sustainable production within the apple production system. In particular, the future policy mix should consider the entirety of the food system including perspectives and requirements of different stakeholders. The three-stage approach applied has demonstrated its feasibility of investigating sustainability issues facing a particular industry within a specific cultural and policy context.
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http://dx.doi.org/10.1016/j.jenvman.2021.114082DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8683745PMC
January 2022

Health Communication About Hospice Care in Chinese Media: Digital Topic Modeling Study.

JMIR Public Health Surveill 2021 10 21;7(10):e29375. Epub 2021 Oct 21.

Department of Public Health and Preventive Medicine, School of Medicine, Jinan University, Guangzhou, China.

Background: Hospice care, a type of end-of-life care provided for dying patients and their families, has been rooted in China since the 1980s. It can improve receivers' quality of life as well as ease their economic burden. The Chinese mass media have continued to actively dispel misconceptions surrounding hospice care and deliver the latest information to citizens.

Objective: This study aims to retrieve and analyze news reports on hospice care in order to gain insight into whether any differences existed in heath information delivered over time and to evaluate the role of mass media in health communication in recent years.

Methods: We searched the Huike (WiseSearch) news database for relevant news reports from Chinese mass media released between 2014 and 2019. We defined two time periods for this study: (1) January 1, 2014, to December 31, 2016, and (2) January 1, 2017, to December 31, 2019. The data cleaning process was completed using Python. We determined appropriate topic numbers for these two periods based on the coherence score and applied latent Dirichlet allocation topic modeling. Keywords for each topic and corresponding topics' names were then generated. The topics were plotted into different circles, and their distances on the 2D plane was represented by multidimensional scaling.

Results: After removing duplicated and irrelevant news articles, we obtained a total of 2227 articles. We chose 8 as the suitable topic number for both study periods and generated topic names and associated keywords. The top 3 most reported topics in the first period were patient treatment, hospice care stories, and development of health care services and health insurance, accounting for 18.68% (178/953), 16.58% (158/953), and 14.17% (135/953) of the collected reports, respectively. The top 3 most reported topics in the second period were hospice care stories, patient treatment, and development of health care services, accounting for 15.62% (199/953), 15.38% (15.38/953), and 14.27% (182/953), respectively.

Conclusions: Topic modeling of news reports gives us a better understanding of the patterns of health communication about hospice care by mass media. Chinese mass media frequently reported on hospice care in April of every year on account of a traditional Chinese festival. Moreover, an increase in coverage was observed in the second period. The two periods shared 6 similar topics, of which patient treatment outstrips hospice care stories was the most reported topic in the second period, implying the humanistic spirit behind the reports. Based on the findings of this study, we suggest stakeholders cooperate with the mass media when planning to update policies.
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http://dx.doi.org/10.2196/29375DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8569548PMC
October 2021

Pain-related health care costs for long-term care residents.

BMC Geriatr 2021 10 14;21(1):552. Epub 2021 Oct 14.

Department of Community Health Sciences, University of Manitoba, S113-750 Bannatyne Ave, Winnipeg, MB, R3E 0W3, Canada.

Background: We tested for differences in direct health care costs among long-term care (LTC) residents age 65 and older with clinically significant pain (CSP) and with no pain or non-daily mild pain (NP/NDMP). We are not aware of any other large scale investigation that examined the cost of pain in LTC environments.

Methods: Population-based administrative health data from Saskatchewan, Canada for 2004 to 2015 were used to compare direct health care costs for CSP and NP/NDMP groups up to one year after admission to LTC. Total accumulated costs for hospitalization, physician services, LTC, and prescription drugs were calculated in 2015 Canadian dollars. Group differences were tested using generalized linear models with generalized estimating equations.

Results: Amongst 24,870 LTC residents, 8289 (33.3%) were censored due to death or discharge in the 365-day study observation period. Of the 16,581 (66.7%) observed residents, 5683 (34.3%) had CSP at admission. Residents (66.3% female) had a mean age of 85 years (SD = 7.4). The mean annual total direct health care cost per resident was higher among the CSP group (CAD $8063) than the NP/NDMP group (CAD $6455). This difference was found even after including LTC costs, and for each cost component (i.e., CSP residents had higher hospitalization, physician, and prescription drug costs). Similar results were obtained after controlling for demographics, comorbidities, physical and cognitive impairment, prior health care costs, and facility characteristics.

Conclusion: The higher costs incurred by CSP residents compared to NP/NDMP residents are likely underestimated because pain problems are often missed in residents with dementia, who comprise a large portion of the LTC population. Improved pain care can reduce such costs and improve quality of life.
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http://dx.doi.org/10.1186/s12877-021-02424-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8515764PMC
October 2021

Rapid Conversion of a Au Ag into a Au Ag Nanocluster via Bisphosphine Ligand Engineering.

Chemistry 2021 Dec 25;27(70):17554-17558. Epub 2021 Oct 25.

Department of Chemistry and Centre for, Atomic Engineering of Advanced Materials, Key Laboratory of Structure and Functional Regulation of Hybrid Materials of Ministry of Education, Institutes of Physical Science and Information Technology and Anhui Province Key Laboratory of Chemistry for, Inorganic/Organic Hybrid Functionalized Materials, Anhui University, Hefei, Anhui, 230601, P. R. China.

The [Au Ag (SAdm) (Dppe) ] nanocluster with aggregation-induced emission (AIE) was synthesized from a non-fluorescent [Au Ag (SAdm) (Dppm) Cl ](SbF ) nanocluster via a ligand-exchange engineering (Dppe=1,2-Bis(diphenylphosphino)ethane, Dppm=Bis(diphenylphosphino)methane, HSAdm=1-Adamantanethiol). The nanocluster has a Au-doped icosahedral Au Ag core, capped by two Ag(SR) , one Ag(SR) and two Dppe ligands. By changing the achiral Dppe ligand into a chiral dbpb ligand ((2S,3S)-(-)-Bis(diphenylphosphino)butane or (2R,3R)-(+)-2,3-Bis(diphenylphosphino)butane), chiral nanoclusters are obtained. ESI-MS and UV-vis spectroscopy were performed to track the reaction. This work provides guidance for the construction of new clusters by etching clusters with multidentate phosphine ligands.
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http://dx.doi.org/10.1002/chem.202102858DOI Listing
December 2021

Discovery of Novel Polycyclic Polyprenylated Acylphloroglucinols from the Fruits of as Antitumor Agents by Suppressing the STAT3 Signaling.

Int J Mol Sci 2021 Sep 26;22(19). Epub 2021 Sep 26.

School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan 430074, China.

Pharmacologic studies have revealed that polycyclic polyprenylated acylphloroglucinols (PPAPs) collectively exhibit a broad range of biological activities, including antineoplastic potential. Here, six new PPAPs, named garcixanthochymones F-K (, , , , , and ), together with nine known analogues were isolated from the fruits of . Their structures were elucidated based on the spectroscopic data, including UV, HRESIMS, and NMR, and quantum chemical calculations. All the isolated PPAPs were tested for anti-proliferative activity against four human tumor cell lines, including SGC7901, A549, HepG2, and MCF-7. Most of the PPAPs possessed high anti-proliferative activity with IC values in the range of 0.89 to 36.98 μM, and significant apoptosis was observed in MCF-7 cells exposed to compounds and . Besides, docking results showed that compounds and could strongly combine with the Src homology 2 (SH2) domain of STAT3 via hydrogen bond and hydrophobic interaction, which is one of the key oncogenes and crucial therapeutic targets. Furthermore, compounds and efficiently downregulated the expression of p-STAT3 and pivotal effector proteins involved in oncogenic signaling pathways of MCF-7 cells.
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http://dx.doi.org/10.3390/ijms221910365DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8508737PMC
September 2021

[Ag(S-Bu)(Dppm)](SbF): an intermediate-sized metalloid silver nanocluster containing a building block of Ag.

Chem Commun (Camb) 2021 Oct 7;57(80):10383-10386. Epub 2021 Oct 7.

Institute of Physical Science and Information Technology, Anhui University, Hefei, Anhui, 230601, P. R. China; Key Laboratory of Structure and Functional Regulation of Hybrid Materials (Anhui University) Ministry of Education, Hefei, 230601, P. R. China.

An intermediate-sized atomically precise metalloid silver nanocluster [Ag(SR)(Dppm)](SbF) (Dppm = bis (diphenylphosphino)methane, SR = S-Bu) is reported, which comprises one building block Ag, six SR pentagons, one sole SR ligand, a DppmAg handle, and an Ag lid. Structurally, a decahedron Ag kernel is observed in the metalloid silver nanocluster. Moreover, the Ag unit provides insights into the growth of large clusters such as Ag(SR)Cl and Ag(SR)Br assembly. The observed decahedron Ag provides a deeper understanding on Marks decahedron in larger nanoclusters, and the [Ag(S-Bu)(Dppm)](SbF) uses Ag as a building block to predict the structure of larger metalloid nanoclusters.
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http://dx.doi.org/10.1039/d1cc04934hDOI Listing
October 2021

Structure Determination of the Cl-Enriched [Ag(SAdm)Cl] Nanocluster.

Inorg Chem 2021 Oct 13;60(19):14803-14809. Epub 2021 Sep 13.

Key Laboratory of Structure and Functional Regulation of Hybrid Materials, Ministry of Education, Anhui University, Hefei, Anhui 230601, P. R. China.

Cl atoms can serve as the innermost core, the peripheral ligand, or the counterions of metal nanoclusters. Herein, we report the structural determination a Cl-enriched [Ag(SAdm)Cl]. The ratio of Cl to AdmSH is quite high compared to those of other nanoclusters. Structurally, nine Cl atoms, existing at the interlayer of the inner kernel and the surface motif, serve as the bridging ligands to sustain the robustness of the whole structure. Interestingly, four Cl atoms on the motif structure can be substituted by Br. This work allows us to clear the regulation of Cl ligands in the structural construction of metal nanoclusters.
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http://dx.doi.org/10.1021/acs.inorgchem.1c02067DOI Listing
October 2021

The effect of retaining intact posterior capsule in congenital cataract surgery in children aged 4-8 years.

BMC Ophthalmol 2021 Sep 11;21(1):332. Epub 2021 Sep 11.

Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital of Capital Medical University, Beijing Key Laboratory of Ophthalmology & Visual Sciences, Beijing, 100005, China.

Background: The present study sought to observe the effect of retaining intact posterior capsule in congenital cataract surgery in children aged 4-8 years.

Methods: This is a retrospective case control study. Seventy-seven children (130 eyes) aged from 4 to 8 years who underwent cataract surgery were divided into two groups. In Group A, 50 eyes underwent phacoemulsification, intraocular lens implantation and posterior capsule capsulotomy combined with anterior vitrectomy. In Group B, 80 eyes underwent cataract phacoemulsification and intraocular lens implantation. The postoperative visual acuity and the rate of complications were compared.

Results: In all patients, cataract surgeries were performed evenly without intraoperative complications. The follow-up time ranged from 6 months to 42 months. No apparent visual axis opacity was detected in group A during the follow-up. By the last visit, apparent visual axis opacity was detected in 31 eyes (38.75%) in group B. Among them, 9 eyes (29.03%) with mild posterior capsule opacification (PCO) were treated with Nd:YAG laser, 3 eyes (9.68%) with thick proliferative membranes were treated with posterior capsule capsulotomy combined with anterior vitrectomy and proliferative membranes in 19 eyes (61.29%) were completely aspired and the posterior capsule was retained. During follow-up, only 2 (6.45%) eyes had PCO recurrence and were treated with Nd:YAG laser. The visual acuity was significantly higher than that before surgery in all patients.

Conclusions: For older children, the incidence of PCO will be low even if intact posterior capsule is retained. Either Nd:YAG laser or surgical treatment for PCO will be able to maintain good vision.
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http://dx.doi.org/10.1186/s12886-021-02098-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8436493PMC
September 2021

Development of influenza-associated disease burden pyramid in Shanghai, China, 2010-2017: a Bayesian modelling study.

BMJ Open 2021 09 8;11(9):e047526. Epub 2021 Sep 8.

School of Public Health, Fudan University, Shanghai, Shanghai, China

Objectives: Negative estimates can be produced when statistical modelling techniques are applied to estimate morbidity and mortality attributable to influenza. Based on the prior knowledge that influenza viruses are hazardous pathogens and have adverse health outcomes of respiratory and circulatory disease (R&C), we developed an improved model incorporating Bayes' theorem to estimate the disease burden of influenza in Shanghai, China, from 2010 to 2017.

Design: A modelling study using aggregated data from administrative systems on weekly R&C mortality and hospitalisation, influenza surveillance and meteorological data. We constrained the regression coefficients for influenza activity to be positive by truncating the prior distributions at zero.

Setting: Shanghai, China.

Participants: People registered with R&C deaths (450 298) and hospitalisations (2621 787, from 1 July 2013), and with influenza-like illness (ILI) outpatient visits (342 149) between 4 January 2010 and 31 December 2017.

Primary Outcome Measures: Influenza-associated disease burden (mortality, hospitalisation and outpatient visit rates) and clinical severity (outpatient-mortality, outpatient-hospitalisation and hospitalisation-mortality risks).

Results: Influenza was associated with an annual average of 15.49 (95% credibility interval (CrI) 9.06-22.06) excess R&C deaths, 100.65 (95% CrI 48.79-156.78) excess R&C hospitalisations and 914.95 (95% CrI 798.51-1023.66) excess ILI outpatient visits per 100 000 population in Shanghai. 97.23% and 80.24% excess R&C deaths and hospitalisations occurred in people aged ≥65 years. More than half of excess morbidity and mortality were associated with influenza A(H3N2) virus, and its severities were 1.65-fold to 3.54-fold and 1.47-fold to 2.16-fold higher than that for influenza A(H1N1) and B viruses, respectively.

Conclusions: The proposed Bayesian approach with reasonable prior information improved estimates of influenza-associated disease burden. Influenza A(H3N2) virus was generally associated with higher morbidity and mortality, and was relatively more severe compared with influenza A(H1N1) and B viruses. Targeted influenza prevention and control strategies for the elderly in Shanghai may substantially reduce the disease burden.
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http://dx.doi.org/10.1136/bmjopen-2020-047526DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438833PMC
September 2021

A Deep Non-negative Matrix Factorization Model for Big Data Representation Learning.

Front Neurorobot 2021 20;15:701194. Epub 2021 Jul 20.

School of Software, Dalian University of Technology, Dalian, China.

Nowadays, deep representations have been attracting much attention owing to the great performance in various tasks. However, the interpretability of deep representations poses a vast challenge on real-world applications. To alleviate the challenge, a deep matrix factorization method with non-negative constraints is proposed to learn deep part-based representations of interpretability for big data in this paper. Specifically, a deep architecture with a supervisor network suppressing noise in data and a student network learning deep representations of interpretability is designed, which is an end-to-end framework for pattern mining. Furthermore, to train the deep matrix factorization architecture, an interpretability loss is defined, including a symmetric loss, an apposition loss, and a non-negative constraint loss, which can ensure the knowledge transfer from the supervisor network to the student network, enhancing the robustness of deep representations. Finally, extensive experimental results on two benchmark datasets demonstrate the superiority of the deep matrix factorization method.
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http://dx.doi.org/10.3389/fnbot.2021.701194DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8329448PMC
July 2021

Ultrastructure evidence for vesicles and double-membrane structures involved in cytoplasmic elimination during spermiogenesis in large yellow croaker, Larimichthys crocea (Teleostei, Perciformes, Scienidae).

Micron 2021 Nov 22;150:103122. Epub 2021 Jul 22.

Key Laboratory of Applied Marine Biotechnology by the Ministry of Education, School of Marine Sciences, Ningbo University, Ningbo, Zhejiang, 315211, People's Republic of China. Electronic address:

Spermatids eliminate excess cytoplasm to form streamlined sperm during spermiogenesis, which mechanism is insufficiently elucidated in fish. In this study, we investigated the cytoplasmic elimination procedure in spermatid during spermiogenesis in the large yellow croaker (Larimichthys crocea) using transmission electron microscopy. The early spermatid is subrotund with a centrally located nucleus. With further development, nucleus polarizes into one side of the cell while the cytoplasm with numerous vesicles near the membrane migrates to the caudal region. Furthermore, exocytosis-like structures were detected in middle spermatid. In late spermatid, the vesicles are reduced and rarely observed. These findings indicate that vesicles may be involved in cytoplasmic elimination possibly via exocytosis. In the later spermatid, a double-membrane, autophagosome-like structure envelopes the cytoplasm, which may develop into a single-membrane structure, and gets discarded from the cell as a residual body from the caudal region. This suggests its potential functions in the formation of residual body and cytoplasmic elimination. Overall, our results revealed that polarized development of spermatid causes polarized distribution of cytoplasm necessary for cytoplasmic elimination. Moreover, they provide ultrastructure evidence for vesicles and double-membrane structures involved in discarding spermatid cytoplasm in large yellow croaker, thus offering novel insights into cytoplasmic elimination during spermiogenesis in fish.
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http://dx.doi.org/10.1016/j.micron.2021.103122DOI Listing
November 2021

PIWI-interacting RNAs are aberrantly expressed and may serve as novel biomarkers for diagnosis of lung adenocarcinoma.

Thorac Cancer 2021 09 3;12(18):2468-2477. Epub 2021 Aug 3.

Department of Respiratory and Critical Care Medicine, Qilu Hospital of Shandong University, Jinan, China.

Background: Lung adenocarcinoma (LUAD) is the main subtype of primary lung cancer and is a leading cause of cancer-related death worldwide. PIWI-interacting RNAs (piRNAs) are a type of small non-coding RNAs that may play crucial roles in cancer progression and serve as biomarkers for tumor detection. This study aimed to explore the expression profiles and diagnostic values of piRNAs in LUAD.

Methods: Small RNA sequencing was performed to investigate tissue piRNA profiles of LUAD. The expression of selected upregulated piRNAs were detected in tissues and serum exosome samples by quantitative real-time polymerase chain reaction (qRT-PCR). Serum exosomes were identified by transmission electron microscope, nanoparticle tracking analysis, and western blot analysis. Receiver operating characteristic (ROC) curve was adopted to quantify the diagnostic potentials of piRNAs in LUAD. Finally, a piRNA panel was developed by multivariate logistic regression model.

Results: We identified that 76 piRNAs were overexpressed and 9 piRNAs were underexpressed in LUAD tissues compared with adjacent non-tumor tissues. Among the top 10 overexpressed piRNAs, 4 piRNAs (piR-hsa-26925, piR-hsa-5444, piR-hsa-30636, and piR-hsa-8757) were verified by qRT-PCR to be significantly upregulated in LUAD tissues. Moreover, piR-hsa-26925 and piR-hsa-5444 had a significantly higher level in serum exosome samples of LUAD patients than those of healthy controls. We finally established a 2-piRNA panel composed of piR-hsa-26925 and piR-hsa-5444, which showed higher diagnostic performance for LUAD with an AUC of 0.833.

Conclusions: Our finding revealed the abnormally expressed piRNAs in LUAD, and serum exosomal piR-hsa-26925 and piR-hsa-5444 could serve as potential biomarkers for LUAD diagnosis.
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http://dx.doi.org/10.1111/1759-7714.14094DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8447905PMC
September 2021

Pterostilbene suppresses oxidative stress and allergic airway inflammation through AMPK/Sirt1 and Nrf2/HO-1 pathways.

Immun Inflamm Dis 2021 12 2;9(4):1406-1417. Epub 2021 Aug 2.

Jilin Key Laboratory for Immune and Targeting Research on Common Allergic Diseases, Yanbian University, Yanji, Jilin, China.

Introduction: Pterostilbene (Pts) may be used for allergic asthma treatment. The AMPK/Sirt1 and Nrf2/HO-1 pathways are potential targets for asthma treatement. However, the relationship between Pts and AMPK/Sirt1 and Nrf2/HO-1 pathways in asthma is unclear. Herein, we aim to explore the pharmacological effects of Pts on oxidative stress and allergic inflammatory response as well as the mechanism involving AMPK/Sirt1 and Nrf2/HO-1 pathways.

Methods: Asthma model was established in mice with ovalbumin (OVA). The model mice were treated by different concentrations of Pts. Lung pathological changes were observed through histological staining. In vitro, lipopolysaccharide (LPS)-stimulated 16HBE cells were treated with Pts. The siAMPKα2, siSirt1 and siNrf2 knockdown, and treatment with compound C, EX-527 or ML385 were also performed in 16HBE cells. Enzyme-linked immunosorbent assay was used to detect interleukin-4 (IL-4), IL-13, IL-5, total and OVA specific immunoglobulin E (IgE), and interferon γ (IFN-γ). Pneumonography was used to measure the airway hyperreactivity (AHR). Superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) levels were also detected. Immunohistochemistry, Western blot and immunofluorescence were used to measure protein levels.

Results: Pts significantly attenuated lung inflammatory cell infiltration and goblet cell proliferation. Meanwhile, Pts treatment could reduce IL-4, IL-13, IL-5, and IgE (total and OVA specific) levels in the asthma model mice. However, IFN-γ in bronchoalveolar lavage fluid was elevated. In addition, Pts reduced AHR. We also found that Pts treatment promoted serum SOD and CAT, and reduced MDA. In vitro results showed that Pts treatment promoted iNOS, TNF-α, COX-2, IL-1β, and IL-6 expressions in 16HBE cells, prolonged G0/G1 phase of the cell cycle, and resulted in a shortened G2M phase. Moreover, we found that Pts promoted the phosphorylation of AMPK in 16HBE, and meanwhile inhibited the increase of ROS induced by LPS. Additionally, Pts treatment inhibited p-AMPK, Sirt1, Nrf2 and HO-1, which in turn leads to the alleviation of AMPK/Sirt1 and Nrf2/HO-1 pathways.

Conclusion: Pts alleviated oxidative stress and allergic airway inflammation via regulation of AMPK/Sirt1and Nrf2/HO-1 signaling pathways.
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http://dx.doi.org/10.1002/iid3.490DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8589405PMC
December 2021

Macro-composition quantification combined with metabolomics analysis uncovered key dynamic chemical changes of aging white tea.

Food Chem 2022 Jan 21;366:130593. Epub 2021 Jul 21.

College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China. Electronic address:

It is a common belief in China that aging could improve the quality of white tea. However, the stored-induced compositional changes remain elusive. In this study, ten subsets of white tea samples, which had been stored for 1-, 2-, 3-, 4-, 5-, 6-, 7-, 10-, 11- and 13- years, were selected. Macro-compositions were quantified firstly. As the results showed, it was interesting to find total flavonoids, thearubigins (TRs), and theabrownines (TBs) increasing, accompanied with a gradual decrease of total polyphenols, which suggest a conversion of phenolic component in the aging process. Then, nontargeted metabolomics was further conducted on selected subsets of samples, including 1-, 7- and 13- years stored to profile their conversion. As a result, most different metabolites were related to flavonol glycosides and flavone glycosides, suggesting dynamic phenolic component changes were vital in aging. The partial least-squares-discriminant analysis (PLS-DA) also identified them as markers in distinguishing.
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http://dx.doi.org/10.1016/j.foodchem.2021.130593DOI Listing
January 2022

Chiral Phosphine-Copper Iodide Hybrid Cluster Assemblies for Circularly Polarized Luminescence.

J Am Chem Soc 2021 Jul 19;143(29):10860-10864. Epub 2021 Jul 19.

Division of Nanomaterials & Chemistry, Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, Anhui 230026, China.

Chiral chromophores and their ordered assemblies are intriguing for yielding circularly polarized luminescence (CPL) and exploring intrinsic structure-light emission relationships. With the extensively studied chiral organic molecules and inorganic nanoparticle assemblies for the amplified CPL, the assemblies of copper halide hybrid clusters have attracted intensive attention due to their potential efficient CPL. Here, we report robust chiral phosphine-copper iodide hybrid clusters and their layered assemblies in crystalline states for amplified CPL. We reveal that the intermolecular interactions endow the clusters with the capability of assembling into chiral crystalline CPL materials, including hexagonal platelet-shaped microcrystals ( ≈ 9.5 × 10) and highly oriented crystalline films ( ≈ 5 × 10). Owing to the high crystalline feature of the thin film, we demonstrate an electroluminescent device with bright electroluminescence (1200 cd m).
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http://dx.doi.org/10.1021/jacs.1c05476DOI Listing
July 2021

Anisotropic Evolution of Nanoclusters from Ag to Ag: Halogen- and Defect-Induced Epitaxial Growth in Nanoclusters.

J Phys Chem Lett 2021 Jul 13;12(28):6654-6660. Epub 2021 Jul 13.

Key Laboratory of Structure and Functional Regulation of Hybrid Materials of Ministry of Education, Institute of Physical Science and Information Technology, Anhui University, Hefei, Anhui 230601, P. R. China.

Halogens have widely served as handles for regulating the growth of nanoparticles and the control of their physicochemical properties. However, their regulatory mechanism is poorly understood. Nanoclusters are the early morphology of nanoparticles and play an important role in revealing the formation and growth of nanoparticles due to their precise structures. Here, we report that halogens induce the anisotropic growth of Ag(CHCOO)(SR)(CHCN) (, where SR = 4--butylbenzylmercaptan) into Ag(CHCOO)(SR)Cl (), where is converted from Ag(CHCOO)(SR) (). Experiments and theoretical simulations showed that halogen ions adsorb at both ends of the cluster, forming defect sites. The -SR-Ag- complexes fill the defects and complete the anisotropic transition from to . Circular dichroism spectra show that the chirality of decreases 15-fold compared with that of . This work provides important insights into the effects of halogens on the growth mechanism and property regulation for nanomaterials at the atomic level and the benefits of further applications of halogen-induced nanomaterials.
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http://dx.doi.org/10.1021/acs.jpclett.1c01713DOI Listing
July 2021

New atomically precise (M = Au/Ag) nanoclusters as excellent oxygen reduction reaction catalysts.

Chem Sci 2021 Jan 7;12(10):3660-3667. Epub 2021 Jan 7.

Department of Chemistry, Centre for Atomic Engineering of Advanced Materials, Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, Anhui University Hefei Anhui 230601 P. R. China

By introducing 1,1'-bis-(diphenylphosphino)ferrocene (dppf) as an activating ligand, two novel nanoclusters, (M = Au/Ag), have been controllably synthesized and structurally characterized. The atomically precise structures of the nanoclusters were determined by SCXC and further confirmed by ESI-TOF-MS, TGA, XPS, DPV, and FT-IR measurements. The nanoclusters supported on activated carbon (C) are exploited as efficient oxygen reduction reaction (ORR) catalysts in alkaline solutions. Density functional theory (DFT) calculations verify that the catalytic activities of the two cluster-based systems originate from the significant ensemble synergy effect between the M kernel and dppf ligand in . This work sheds lights on the preparation of cluster-based electrocatalysts and other catalysts that are activated and modified by peripheral ligands.
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http://dx.doi.org/10.1039/d0sc05923dDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179487PMC
January 2021

YWHAE as an HE4 interacting protein can influence the malignant behaviour of ovarian cancer by regulating the PI3K/AKT and MAPK pathways.

Cancer Cell Int 2021 Jun 9;21(1):302. Epub 2021 Jun 9.

Department of Obstetrics and Gynecology, Shengjing Hospital Affiliated to China MedicalUniversity, No. 36, Sanhao Street, Heping District, Shenyang, 110004, People's Republic of China.

Background: Malignant tumours of the female reproductive system threaten the lives and health of women worldwide, with ovarian cancer having the highest mortality rate. Based on previous work, this study analysed the expression and role of YWHAE in ovarian epithelial tumours.

Methods: The interaction between YWHAE and HE4 was evaluated via immunoprecipitation, western blot analysis, and cellular immunofluorescence. Immunohistochemistry was used to address the relationship between YWHAE expression, clinicopathological parameters, and patient prognosis. Changes in cell invasion, epithelial-mesenchymal transition, migration, proliferation, apoptosis, and cell cycle before and after differential expression of YWHAE were also explored in ovarian cancer cell lines and via in vivo experiments.

Results: YWHAE was found to interact with HE4, and its expression was positively correlated with HE4 expression. Moreover, YWHAE upregulation was associated with advanced stages of ovarian cancer and poor patient prognosis. In addition, YWHAE enhanced invasion, migration, and proliferation, but inhibited the apoptosis of ovarian cancer cells. These biological effects were found to be mediated by the AKT and MAPK signalling pathways.

Conclusions: Altogether, this study demonstrates that YWHAE is substantially upregulated in ovarian cancer tissues, representing a risk factor for the prognosis of ovarian cancer that is positively correlated with HE4 expression. Furthermore, YWHAE and its downstream pathways may represent new therapeutic targets for ovarian cancer.
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http://dx.doi.org/10.1186/s12935-021-01989-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190858PMC
June 2021
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