Shahram Misaghi

Shahram Misaghi

UNVERIFIED PROFILE

Are you Shahram Misaghi?   Register this Author

Register author
Shahram Misaghi

Shahram Misaghi

Publications by authors named "Shahram Misaghi"

Are you Shahram Misaghi?   Register this Author

33Publications

1009Reads

26Profile Views

Utilizing a regulated targeted integration cell line development approach to systematically investigate what makes an antibody difficult to express.

Biotechnol Prog 2019 03 10;35(2):e2772. Epub 2019 Jan 10.

Dept. of Cell Culture, Genentech Inc., South San Francisco, California, 94080.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/btpr.2772DOI Listing
March 2019

High Intracellular Seed Train BiP Levels Correlate With Poor Production Culture Performance in CHO Cells.

Biotechnol J 2018 Oct 24;13(10):e1700746. Epub 2018 Apr 24.

Department of Early Stage Cell Culture, Genentech, Inc., South San Francisco, CA, 94080, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/biot.201700746DOI Listing
October 2018

Beating the odds: The poisson distribution of all input cells during limiting dilution grossly underestimates whether a cell line is clonally-derived or not.

Biotechnol Prog 2018 05 9;34(3):559-569. Epub 2017 Oct 9.

Early Stage Cell Culture (ESCC) Department, Genentech, Inc., 1 DNA Way, South San Francisco, California, 94080.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/btpr.2560DOI Listing
May 2018

Supplementation of Nucleosides During Selection can Reduce Sequence Variant Levels in CHO Cells Using GS/MSX Selection System.

Biotechnol J 2018 Jan 17;13(1). Epub 2017 Aug 17.

Department of Early Stage Cell Culture, Genentech, Inc., South San Francisco, CA, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/biot.201700335DOI Listing
January 2018

Evaluation of heavy chain C-terminal deletions on productivity and product quality of monoclonal antibodies in Chinese hamster ovary (CHO) cells.

Biotechnol Prog 2017 May 24;33(3):786-794. Epub 2017 Mar 24.

Dept. of Early Stage Cell Culture, Genentech Inc, 1 DNA Way, South San Francisco, CA, 94080.

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1002/btpr.2444
Publisher Site
http://dx.doi.org/10.1002/btpr.2444DOI Listing
May 2017

Slashing the timelines: Opting to generate high-titer clonal lines faster via viability-based single cell sorting.

Biotechnol Prog 2016 Jan-Feb;32(1):198-207. Epub 2015 Dec 12.

Dept. of Immunology, Genentech, Inc, 1 DNA Way, South San Francisco, CA, 94080.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/btpr.2204DOI Listing
November 2016

Carboxypeptidase D is the only enzyme responsible for antibody C-terminal lysine cleavage in Chinese hamster ovary (CHO) cells.

Biotechnol Bioeng 2016 10 6;113(10):2100-6. Epub 2016 Apr 6.

Department of Early Stage Cell Culture, Genentech Inc., 1 DNA Way, South San Francisco, California 94080.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/bit.25977DOI Listing
October 2016

NLRP3 recruitment by NLRC4 during Salmonella infection.

J Exp Med 2016 05 2;213(6):877-85. Epub 2016 May 2.

Department of Physiological Chemistry, Genentech Inc., South San Francisco, CA 94080

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1084/jem.20132234DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4886354PMC
May 2016

It's time to regulate: coping with product-induced nongenetic clonal instability in CHO cell lines via regulated protein expression.

Biotechnol Prog 2014 Nov-Dec;30(6):1432-40. Epub 2014 Aug 16.

Dept. of Early Stage Cell Culture, Genentech, Inc, 1 DNA way, South San, Francisco, CA, 94080.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/btpr.1970DOI Listing
August 2015

Resilient immortals, characterizing and utilizing Bax/Bak deficient Chinese hamster ovary (CHO) cells for high titer antibody production.

Biotechnol Prog 2013 May-Jun;29(3):727-37. Epub 2013 Apr 18.

Dept. of Early Stage Cell Culture, Genentech, Inc., 1 DNA way, South San Francisco, CA, 94080, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/btpr.1722DOI Listing
January 2014

Phosphorylation of NLRC4 is critical for inflammasome activation.

Nature 2012 Oct 12;490(7421):539-42. Epub 2012 Aug 12.

Department of Physiological Chemistry, Genentech Inc., 1 DNA Way, South San Francisco, California 94080, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1038/nature11429DOI Listing
October 2012

Pannexin-1 is required for ATP release during apoptosis but not for inflammasome activation.

J Immunol 2011 Jun 20;186(11):6553-61. Epub 2011 Apr 20.

Physiological Chemistry Department, Genentech, Inc, South San Francisco, CA 94080, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.4049/jimmunol.1100478DOI Listing
June 2011

Prolonged endoplasmic reticulum stress promotes mislocalization of immunoglobulins to the cytoplasm.

Mol Immunol 2010 May 28;47(9):1719-27. Epub 2010 Mar 28.

The Institute of Drug Research, The School of Pharmacy, The Hebrew University, Jerusalem 91120, Israel.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.molimm.2010.03.006DOI Listing
May 2010

Glyburide inhibits the Cryopyrin/Nalp3 inflammasome.

J Cell Biol 2009 Oct;187(1):61-70

Department of Physiological Chemistry, Genentech, South San Francisco, CA 94080, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1083/jcb.200903124DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2762099PMC
October 2009

DNA targets of AID evolutionary link between antibody somatic hypermutation and class switch recombination.

Adv Immunol 2009 ;101:163-89

Genentech, Immunology Discovery Group, South San Francisco, California 94080, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/S0065-2776(08)01005-5DOI Listing
April 2009

Sequence and structure evolved separately in a ribosomal ubiquitin variant.

EMBO J 2007 Jul 28;26(14):3474-83. Epub 2007 Jun 28.

Department of Biology, Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Cambridge, MA 02142, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1038/sj.emboj.7601772DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1933403PMC
July 2007

ElaD, a Deubiquitinating protease expressed by E. coli.

PLoS One 2007 Apr 18;2(4):e381. Epub 2007 Apr 18.

Immunology Program, Harvard Medical School, Boston, Massachusetts, United States of America.

View Article

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0000381PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1847702PMC
April 2007

Chlamydia trachomatis infection induces cleavage of the mitotic cyclin B1.

Infect Immun 2006 Oct;74(10):5602-8

Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Ave., Boston, MA 02115, USA.

View Article

Download full-text PDF

Source
http://iai.asm.org/cgi/doi/10.1128/IAI.00266-06
Publisher Site
http://dx.doi.org/10.1128/IAI.00266-06DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1594933PMC
October 2006

Identification by functional proteomics of a deubiquitinating/deNeddylating enzyme in Plasmodium falciparum.

Mol Microbiol 2006 Sep;61(5):1187-95

Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1365-2958.2006.05307.xDOI Listing
September 2006

Chlamydia trachomatis-derived deubiquitinating enzymes in mammalian cells during infection.

Mol Microbiol 2006 Jul;61(1):142-50

Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, 02142, USA.

View Article

Download full-text PDF

Source
http://doi.wiley.com/10.1111/j.1365-2958.2006.05199.x
Publisher Site
http://dx.doi.org/10.1111/j.1365-2958.2006.05199.xDOI Listing
July 2006

Using a small molecule inhibitor of peptide: N-glycanase to probe its role in glycoprotein turnover.

Chem Biol 2004 Dec;11(12):1677-87

Department of Pathology, Harvard Medical School, Boston, MA 02115, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chembiol.2004.11.010DOI Listing
December 2004

Using a small molecule inhibitor of peptide: N-glycanase to probe its role in glycoprotein turnover.

Chem Biol 2004 Dec;11(12):1677-87

Department of Pathology, Harvard Medical School, Boston, MA 02115, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chembiol.2004.11.010DOI Listing
December 2004

Yeast N-glycanase distinguishes between native and non-native glycoproteins.

EMBO Rep 2004 Feb 9;5(2):201-6. Epub 2004 Jan 9.

Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1038/sj.embor.7400066DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1298977PMC
February 2004