Publications by authors named "Shadab Dabagh"

4 Publications

  • Page 1 of 1

Papain grafted into the silica coated iron-based magnetic nanoparticles 'IONPs@SiO-PPN' as a new delivery vehicle to the HeLa cells.

Nanotechnology 2020 May 24;31(19):195603. Epub 2020 Jan 24.

Isfahan Clinical Toxicology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran. Institute of Bioproduct Development, Department of Bioprocess Engineering, Faculty of Chemical and Energy Engineering, Universiti Teknologi Malaysia, Skudai 81110, Johor Bahru, Johor, Malaysia.

The present study aims at engineering, fabrication, characterization, and qualifications of papain (PPN) conjugated SiO-coated iron oxide nanoparticles 'IONPs@SiO-PPN'. Initially fabricated iron oxide nanoparticles (IONPs) were coated with silica (SiO) using sol-gel method to hinder the aggregation and to enhance biocompatibility. Next, PPN was loaded as an anticancer agent into the silica coated IONPs (IONPs@SiO) for the delivery of papain to the HeLa cancer cells. This fabricated silica-coated based magnetic nanoparticle is introduced as a new physiologically-compatible and stable drug delivery vehicle for delivering of PPN to the HeLa cancer cell line. The IONPs@SiO-PPN were characterized using FT-IR, AAS, FESEM, XRD, DLS, and VSM equipment. Silica was amended on the surface of iron oxide nanoparticles (IONPs, γ-FeO) to modify its biocompatibility and stability. The solvent evaporation method was used to activate PPN vectorization. The following tests were performed to highlight the compatibility of our proposed delivery vehicle: in vitro toxicity assay, in vivo acute systemic toxicity test, and the histology examination. The results demonstrated that IONPs@SiO-PPN successfully reduced the IC values compared with the native PPN. Also, the structural alternations of HeLa cells exposed to IONPs@SiO-PPN exhibited higher typical hallmarks of apoptosis compared to the cells treated with the native PPN. The in vivo acute toxicity test indicated no clinical signs of distress/discomfort or weight loss in Balb/C mice a week after the intravenous injection of IONPs@SiO (10 mg kg). Besides, the tissues architectures were not affected and the pathological inflammatory alternations detection failed. In conclusion, IONPs@SiO-PPN can be chosen as a potent candidate for further medical applications in the future, for instance as a drug delivery vehicle or hyperthermia agent.
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http://dx.doi.org/10.1088/1361-6528/ab6fd4DOI Listing
May 2020

Effect of Sintering on Di-Electric Characteristics of Al-Cu Doped Cobalt Ferrite Nanoparticles.

J Nanosci Nanotechnol 2019 Jul;19(7):4142-4146

Laser Center, Ibnu Sina Institute for Scientific and Industrial Research, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia.

Aluminium substituted cobalt-copper CoCuFeAlO₄, (x ═ 0.8) nanoparticles are grown and sintered at different temperature in the range 600 to 900 °C. XRD analysis on nanoparticles prepared at sintered temperatures of 700 °C and 800 °C confirms the spinel structure and presence of hematite phase (alpha ferrite) in them. The dielectric behaviour of the prepared nano-particles is investigated. Although crystallinity improved with increase in sintering temperature and there was a dielectric loss at higher probe analyser frequency. The synthesized nanoparticles an average particle size of 20-24 nm while the FTIR absorption in regions of 586-595 cm and 450-460 cm indicated the presence of intrinsic vibrations of the tetrahedral and octahedral complexes respectively. Electrical resistivity as a function of temperature confirms the semiconducting nature of the Cu-Al substituted cobalt ferrite, and is attributed to the hopping mechanism between Fe Fe ions and Co Cu, Co Al. The lower values of dielectric constants and dielectric losses make Al-Cu doped cobalt ferrite, a potential material for microwave and radio wave absorber applications.
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http://dx.doi.org/10.1166/jnn.2019.16331DOI Listing
July 2019

Synthesis, characterization and in vitro evaluation of exquisite targeting SPIONs-PEG-HER in HER2+ human breast cancer cells.

Nanotechnology 2016 Mar 10;27(10):105601. Epub 2016 Feb 10.

Department of Bioprocess Engineering, Faculty of Chemical Engineering, Universiti Teknologi Malaysia, Skudai 81310, Johor Bahru, Johor, Malaysia.

A stable, biocompatible and exquisite SPIONs-PEG-HER targeting complex was developed. Initially synthesized superparamagnetic iron oxide nanoparticles (SPIONs) were silanized using 3-aminopropyltrimethoxysilane (APS) as the coupling agent in order to allow the covalent bonding of polyethylene glycol (PEG) to the SPIONs to improve the biocompatibility of the SPIONs. SPIONs-PEG were then conjugated with herceptin (HER) to permit the SPIONs-PEG-HER to target the specific receptors expressed over the surface of the HER2+ metastatic breast cancer cells. Each preparation step was physico-chemically analyzed and characterized by a number of analytical methods including AAS, FTIR spectroscopy, XRD, FESEM, TEM, DLS and VSM. The biocompatibility of SPIONs-PEG-HER was evaluated in vitro on HSF-1184 (human skin fibroblast cells), SK-BR-3 (human breast cancer cells, HER+), MDA-MB-231 (human breast cancer cells, HER-) and MDA-MB-468 (human breast cancer cells, HER-) cell lines by performing MTT and trypan blue assays. The hemolysis analysis results of the SPIONs-PEG-HER and SPIONs-PEG did not indicate any sign of lysis while in contact with erythrocytes. Additionally, there were no morphological changes seen in RBCs after incubation with SPIONs-PEG-HER and SPIONs-PEG under a light microscope. The qualitative and quantitative in vitro targeting studies confirmed the high level of SPION-PEG-HER binding to SK-BR-3 (HER2+ metastatic breast cancer cells). Thus, the results reflected that the SPIONs-PEG-HER can be chosen as a favorable biomaterial for biomedical applications, chiefly magnetic hyperthermia, in the future.
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http://dx.doi.org/10.1088/0957-4484/27/10/105601DOI Listing
March 2016

Impact of annealing on surface morphology and photoluminescence of self-assembled Ge and Si quantum dots.

J Nanosci Nanotechnol 2014 Jul;14(7):5266-71

Controlled growth and characterization of germanium (Ge) and silicon (Si) nanostructure are the key issues for optoelectronic device fabrication. The role of post-annealing on the structural and optical properties of radio frequency (rf) magnetron sputtering grown of Ge and Si quantum dots (QDs) deposited on Si(100) substrate is studied. Atomic force microscopy confirmed the formation of Si and Ge QDs with estimated sizes lower than -17 nm and -14 nm respectively. The X-ray diffraction analysis revealed the formation of Si and Ge QDs accompanied by SiO2 with estimated sizes of -5 and -7 nm for post-annealed Si and pre-annealed Ge QDs respectively. The room temperature photoluminescence spectra for Ge and Si demonstrated an emission peak at 3.20 and 2.72 eV respectively, which are attributed to the electron and hole recombination within QDs. A shift in the PL peak is observed through annealing which is ascribable to the changes in size of QDs and quantum confinement effect. The thermal annealing at 600 degrees C is found to play an important role in controlling the shape, number density, root mean square (rms) roughness and the energy shift of the luminescence band for both Si and Ge QDs. The influence of annealing on growth morphology for Ge QDs is appeared to be stronger than Si.
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http://dx.doi.org/10.1166/jnn.2014.8710DOI Listing
July 2014