Publications by authors named "Seyyed Amir Yasin Ahmadi"

11 Publications

  • Page 1 of 1

Designing a Logistic Regression Model for a Dataset to Predict Diabetic Foot Ulcer in Diabetic Patients: High-Density Lipoprotein (HDL) Cholesterol Was the Negative Predictor.

J Diabetes Res 2021 16;2021:5521493. Epub 2021 Mar 16.

Endocrine Research Center, Institute of Endocrinology and Metabolism, Iran University of Medical Sciences, Tehran, Iran.

Objectives: Although the risk factors for diabetic neuropathy and diabetic foot ulcer have been detected, there was no practical modeling for their prediction. We aimed to design a logistic regression model on an Iranian dataset to predict the probability of experiencing diabetic foot ulcers up to a considered age in diabetic patients.

Methods: The present study was a statistical modeling on a previously published dataset. The covariates were sex, age, body mass index (BMI), fasting blood sugar (FBS), hemoglobin A1C (HbA1C), low-density lipoprotein (LDL), high-density lipoprotein (HDL), triglyceride (TG), insulin dependency, and statin use. The final model of logistic regression was designed through a manual stepwise method. To study the performance of the model, an area under receiver operating characteristic (AUC) curve was reported. A scoring system was defined according to the coefficients to be used in logistic function for calculation of the probability.

Results: The pretest probability for the outcome was 30.83%. The final model consisted of age (1 = 0.133), BMI (2 = 0.194), FBS (3 = 0.011), HDL (4 = -0.118), and insulin dependency (5 = 0.986) ( < 0.1). The performance of the model was definitely acceptable (AUC = 0.914).

Conclusion: This model can be used clinically for consulting the patients. The only negative predictor of the risk is HDL cholesterol. Keeping the HDL level more than 50 (mg/dl) is strongly suggested. Logistic regression modeling is a simple and practical method to be used in the clinic.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1155/2021/5521493DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7994070PMC
March 2021

Association of gene polymorphisms and serum E-Selectin level with risk of coronary artery disease in lur population of Iran.

Arch Physiol Biochem 2020 Oct 6:1-6. Epub 2020 Oct 6.

Cardiovascular Research Center, Shahid Rahimi Hospital, Lorestan University of Medical Sciences, Khorramabad, Iran.

Background: Adhesion molecules like E-selectin have important role in pathogenesis of atherosclerosis. and polymorphisms of gene and E-selectin serum level may affect the risk of coronary artery disease (CAD).

Methods: A total of 145 normal individuals and 154 patients diagnosed with CAD from the Lur population of Iran undergoing coronary angiography were enrolled. Genetic polymorphisms of were determined using PCR-RFLP. Serum level of soluble E-selectin was measured using Elisa.

Results: T allele in polymorphism was significantly associated with an increased risk of atherosclerosis ( = 0.018). No significant association was observed for polymorphism. The mean serum level of soluble E-selectin in the patient group was significantly higher than the control group ( < 0.001).

Conclusions: Allele type in polymorphism plays a role in increasing the risk of developing CAD. Furthermore, since serum E-selectin level is associated with systemic inflammation, it contributes to the increased risk of the disease.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/13813455.2020.1828481DOI Listing
October 2020

Pre-Implantation Effects of Progesterone Administration on Ovarian Angiogenesis after Ovarian Stimulation: A Histological, Hormonal, and Molecular Analysis.

JBRA Assist Reprod 2020 Jul 14;24(3):289-295. Epub 2020 Jul 14.

Department of Anatomical Sciences, Lorestan University of Medical Sciences, Khorramabad, Iran.

Objective: Progesterone (P4) is known to directly affect ovarian tissue angiogenesis. The present study was designed to show how P4 affects ovarian angiogenesis in hormonal, histological, and molecular levels.

Methods: Fifteen adult female NMRI mice were divided into three groups: Control Group; Case Group I (ovarian stimulation alone); and Case Group II (ovarian stimulation followed by P4 administration). Blood and ovarian tissue samples were assessed for hormonal, histological, and molecular alterations. Gene expression for ovarian vascular endothelium growth factor (VEGF) and hypoxia-inducible factor-1 alpha (HIF-1α) was analyzed using real-time PCR.

Results: Ovarian hormone levels were increased in the case groups compared with the control group (p<0.05). Quantitative corpus luteum parameters were increased in the case groups compared with the control group (p<0.05). Quantitative ovarian vascular parameters were significantly different in the case groups compared with the control group. Gene expression analyses shows that the mice in Case Group I had higher levels of ovarian VEGF expression than the mice in the control group (p<0.05). No significant difference in gene expression was observed for HIF-1ɑ.

Conclusion: Treatment with P4 after ovarian stimulation enhanced ovarian angiogenesis by increasing hormone levels and causing significant structural changes.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.5935/1518-0557.20190076DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7365533PMC
July 2020

Recurrent Spontaneous Abortion (RSA) and Maternal KIR Genes: A Comprehensive Meta-Analysis.

JBRA Assist Reprod 2020 05 1;24(2):197-213. Epub 2020 May 1.

Student Research Committee, Iran University of Medical Sciences, Tehran, Iran.

Natural killer cells (NKs) are the most important cells in the fetomaternal immune tolerance induced through interaction of maternal killer-cell immunoglobulin-like receptors (KIR) and fetal human leucocyte antigens (HLA). Hence, we intend to perform a meta-analysis on the role of maternal KIR genes diversity in recurrent spontaneous abortion (RSA). The present paper is a meta-analysis of previous genetic association studies and our previous original study. The results showed that KIR3DL1 was a significantly protecting factor for RSA (p=0.044; OR=0.833 [0.698-0.995]; fixed effect model). KIR2DS2 (p=0.034; OR=1.195 [1.013-1.408]; fixed effect model) and KIR2DS3 (p=0.013; OR=1.246 [1.047-1.483]; fixed effect model) were significantly risk factors for RSA. For KIR2DS1 there was a high heterogeneity and publication bias. Briefly, the inhibitory gene KIR3DL1 was a protecting factor, and the activating genes KIR2DS2 and KIR2DS3 were risk factors for RSA. However, the effect sizes were not suitable. We suggest further studies on different causes of pregnancy loss, to find the role of KIR2DS1.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.5935/1518-0557.20190067DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7169921PMC
May 2020

Effect of selenium on freezing-thawing damage of mice spermatogonial stem cell: a model to preserve fertility in childhood cancers.

Stem Cell Investig 2019 26;6:36. Epub 2019 Nov 26.

Department of Anatomical Sciences, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Background: During treatment of childhood cancers, fertility of boys may be affected. Therefore, freezing spermatogonial stem cell (SSC) is recommended. However, freezing-thawing process may cause damage to SSCs. This study was conducted to evaluate protective effects of selenium on freezing-thawing damage of mice SSCs using investigation of cell viability and investigation of apoptosis related genes expression including , , , and .

Methods: SSCs were extracted from 80 6-day-old mice. The SSCs were divided into four groups: cryopreservation along with selenium (low and high dose), vitrification along with selenium (low and high dose), cryopreservation control, and vitrification control. Trypan blue staining and real-time polymerase chain reaction (real-time PCR) were used to investigate cell viability and gene expression, respectively.

Result: Comparison of cell viability in the experimental groups did not show a significant association. Expression of and was significantly lower in cryopreservation group with low-dose selenium. Expression of was significantly lower in cryopreservation group with high-dose selenium. Expression of and was significantly lower in vitrification group with low-dose selenium, and expression of was significantly upper. Expression of and was significantly lower in vitrification group with high-dose selenium, and expression of was significantly upper (P<0.001).

Conclusions: Selenium had dose dependent effect on apoptosis related genes profile. The only evident effect was the effect of low-dose selenium in cryopreservation on inhibition of apoptosis via extrinsic pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/sci.2019.10.01DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6917556PMC
November 2019

An introduction to the role of immunology in medical anthropology and molecular epidemiology.

Biomed Pharmacother 2019 Jan 28;109:2203-2209. Epub 2018 Nov 28.

Research Office for the History of Persian Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran; Department of Anatomical Sciences, Lorestan University of Medical Sciences, Khorramabad, Iran. Electronic address:

Medical anthropology is a multi-disciplinary approach to the medical sciences and humanities. Immunology is of the basic medical sciences dealing with anthropology as a science which involves in recognition of self and non-self. We performed this review paper to introduce the role of immunology in medical anthropology and molecular epidemiology. This narrative review was based on the authors' original experience and current literature. We discussed about human leukocyte antigens (HLA) and killer-cell immunoglobulin-like receptors (KIR) and their disease associations. Bioinformatics and biostatistics help us to use this topic in evidence-based medicine. Immunogenetics is an important part of the molecular anthropology being a part of medical anthropology in turn. There were different notions of the integration of immunology and medical anthropology including environmental, ecological and cultural effects, historical and philosophical approaches, immunological biomarkers in different patients, and immunogenetics. Such studies can be used in pharmacogenomics and personalized medicine especially for immunotherapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biopha.2018.11.085DOI Listing
January 2019

Association of vitamin D level and vitamin D deficiency with risk of preeclampsia: A systematic review and updated meta-analysis.

Taiwan J Obstet Gynecol 2018 Apr;57(2):241-247

Department of Immunology, Lorestan University of Medical Sciences, Khorramabad, Iran. Electronic address:

Objectives: Because of the immune modulatory effects of vitamin D3 in preeclampsia, we intend to have a systematic review and meta-analysis on association of both 25-hydroxy vitamin D (25-OHD) level (parametric approach) and 25-OHD deficiency (non-parametric approach) with preeclampsia. As well, for the parametric part, we used receiver operating characteristic (ROC) curve model.

Materials And Methods: We used Web of Science, PubMed and Science Direct data bases through searching in titles. Google Scholar search engine was used in order to find missing papers. Finally 23 studies were imported. Both random and fixed models were reported.

Results: Based on the forest plot, lower levels of 25-OHD were significantly associated with risk of preeclampsia (fixed and random P < 0.001). Based on the forest plot, vitamin D deficiency (25-OHD < 20 ng/ml) was significantly associated with risk of preeclampsia (fixed P < 0.0001; random P = 0.0029; fixed OR = 1.33; random OR = 1.54). Based on ROC curve results, we found 2 cutoffs of 10.60 and 20.05 ng/ml.

Conclusion: Women with vitamin D deficiency at cutoff 20 ng/ml are more at risk of preeclampsia. This association can be specific up to 90% at 10.60 ng/ml cutoff. Treatment of vitamin D deficiency is necessary before pregnancy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.tjog.2018.02.013DOI Listing
April 2018

Protective effects of melatonin and ghrelin on spermatogenesis: A narrative review of the literature.

Int J Reprod Biomed 2017 May;15(5):265-272

Department of Veterinary Pathology, Islamic Azad University, Tabriz Branch, Tabriz, Iran.

Spermatocytogenesis starts from lumens of seminiferous cords and after migration to the basal membrane ends to the lumens again. We attempt to review the protective effects of melatonin and ghrelin on Spermatocytogenesis and in particular on spermatogonial stem cells, as two rather newly-discovered hormones. Testicular freezing prior to chemotherapy and radiotherapy is one of the ways of preserving fertility in children with cancer. The freezing has two methods of slow-freezing (cryopreservation) and rapid-freezing (vitrification). Administration of melatonin can maintain the quality of the germ cells underwent such processes, as well as ghrelin, can protect germ cells from the toxicities secondary to ischemic injuries, and pathologic apoptosis. This review indicates that in vitro or in vivo administration of melatonin or ghrelin, could be effective to preserve fertilization and also they can be used in assisted reproductive technologies to improve the quality of sperms. Future original studies should be propelled toward human studies, of course with observing the ethics.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5510579PMC
May 2017

A genomic study on distribution of () and alleles in Lak population of Iran.

Genom Data 2017 Mar 10;11:3-6. Epub 2016 Nov 10.

Research Office for the History of Persian Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran; Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran.

Anthropological studies based on the highly polymorphic gene, (), provide useful information for bone marrow donor registry, forensic medicine, disease association studies, as well as infertility treatment, designing peptide vaccines against tumors, and infectious or autoimmune diseases. The aim of this study was to determine and allele frequencies in 100 unrelated Lak/lᴂk/individuals from Lorestan province of Iran. Finally, we compared the results with that previously described in Iranian population. Commercial HLA-Type kits from BAG (Lich, Germany) company were used for determination of the and allele frequencies in genomic DNA, based on polymerase chain reaction with sequence specific primer (PCR-SSP) assay. The differences between the populations in distribution of and alleles were estimated by chi-squared test with Yate's correction. The most frequent alleles were *24 (20%), *02 (18%), *03 (12%) and *11 (10%), and the most frequent alleles were *35 (24%), *51 (16%), *18 (6%) and *38 (6%) in Lak population. *66 (1%), *74(1%) and *48 (1%), *55(1%) were the least observed frequencies in Lak population. Our results based on and allele frequencies showed that Lak population possesses the previously reported general features of Iranians but still with unique.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.gdata.2016.11.012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5122700PMC
March 2017

Colorectal cancer and the genes in the human genome: A meta-analysis.

Genom Data 2016 Dec 1;10:118-126. Epub 2016 Nov 1.

Research Office for the History of Persian Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.

Colorectal cancer is one of the most common types of inflammation-based cancers and is occurred due to growth and spread of cancer cells in colon and/or rectum. Previously genetic association of cell cycle genes, both proto-oncogenes and the tumor suppressors has been proved. But there were few studies about association of immune related genes such as . Thus we intend to perform a meta-analysis to find the association of different genes of and susceptibility to be affected by colorectal cancer. The overall population of the four studies investigated in our meta-analysis was 953 individuals (470 individuals with colorectal cancer and 483 individuals in control groups). After the analyses, we concluded that colorectal cancer is affected by and also there were no protecting gene. This result shows the inflammatory basis of this cancer. In other words, in contrast to leukemia and blood cancers, colorectal cancers seem to be affected by hyper activity of natural killer-cells (NKs). Whys and therefore of this paradox, is suggested to be investigated further.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.gdata.2016.10.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5099266PMC
December 2016

Multiple sclerosis is accompanied by lack of gene: A meta-analysis.

Genom Data 2016 Dec 28;10:75-78. Epub 2016 Sep 28.

Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran.

Multiple sclerosis (MS) is a disease in which we can recognize destruction of the myelin that is around nerve cells of brain and spinal cord called as oligodendrocytes. Both genetic and environmental factors play roles in MS. One of these genes is the -- () which expressed on surface of natural killer cells (NKs). These genes have loci (not locus) in human genome, so they inherit as haplotypes. The results of previous studies show that different genes of may affect both susceptibility and resistance to such autoimmune disorders that their pathogenesis in MS is still unclear. Since NKs play key roles in immune tolerance, we intend to perform a meta-analysis for the correlation of genes and MS. We used the software comprehensive meta-analysis for data of totally 568 MS patients and 280 controls. Among the 14 genes of in the human genome, lack of is accompanied by MS. No gene found to be a risk factor for MS. Further studies on other molecules of NKs like CD94 and NKG2a is suggested.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.gdata.2016.09.009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5054263PMC
December 2016