Publications by authors named "Seokhwan Chung"

3 Publications

  • Page 1 of 1

Microwave-Assisted Synthesis of Carbon Dot - Iron Oxide Nanoparticles for Fluorescence Imaging and Therapy.

Front Bioeng Biotechnol 2021 6;9:711534. Epub 2021 Jul 6.

Department of Materials Science and Engineering, University of Washington, Seattle, WA, United States.

Fluorescence microscopy is commonly used to image specific parts of a biological system, and is applicable for early diagnosis of cancer. Current fluorescent probes, such as organic dyes and quantum dots, suffer from poor solubility and high toxicity, respectively, demonstrating a need for a colloidal stable and non-toxic fluorescent probe. Here we present an iron oxide and carbon dot (CD) based nanoparticle (CNPCP) that displays optical properties similar to those of conventional fluorescent probe and also exhibits good biocompatibility. Fluorescent CDs were synthesized from glucosamine onto chitosan - polyethylene glycol (PEG) graft copolymer using microwave irradiation. These NPs were monodispersed in aqueous environments and displayed excitation-dependent fluorescence; they demonstrated good size stability and fluorescence intensity in biological media. evaluation of CNP as fluorescent probes in cancer cell lines showed that these NPs caused little toxicity, and allowed fast and quantitative imaging. Model therapeutic doxorubicin (DOX) was conjugated onto the NPs (CNPCP-DOX) to demonstrate the multifunctionality of the NPs, and studies showed that CNPCP-DOX was able to kill cancer cells in a dose dependent manner. These results indicate the potential of using CNPCPs as fluorescent probes capable of delivering chemotherapeutics.
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http://dx.doi.org/10.3389/fbioe.2021.711534DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290417PMC
July 2021

Iron oxide nanoparticles for immune cell labeling and cancer immunotherapy.

Nanoscale Horiz 2021 09 20;6(9):696-717. Epub 2021 Jul 20.

Department of Materials Science and Engineering, University of Washington, Seattle, Washington 98195, USA.

Cancer immunotherapy is a novel approach to cancer treatment that leverages components of the immune system as opposed to chemotherapeutics or radiation. Cell migration is an integral process in a therapeutic immune response, and the ability to track and image the migration of immune cells in vivo allows for better characterization of the disease and monitoring of the therapeutic outcomes. Iron oxide nanoparticles (IONPs) are promising candidates for use in immunotherapy as they are biocompatible, have flexible surface chemistry, and display magnetic properties that may be used in contrast-enhanced magnetic resonance imaging (MRI). In this review, advances in application of IONPs in cell tracking and cancer immunotherapy are presented. Following a brief overview of the cancer immunity cycle, developments in labeling and tracking various immune cells using IONPs are highlighted. We also discuss factors that influence the effectiveness of IONPs as MRI contrast agents. Finally, we outline different approaches for cancer immunotherapy and highlight current efforts that utilize IONPs to stimulate immune cells to enhance their activity and response to cancer.
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http://dx.doi.org/10.1039/d1nh00179eDOI Listing
September 2021

Graphene Quantum Dots and Their Applications in Bioimaging, Biosensing, and Therapy.

Adv Mater 2021 Jun 12;33(22):e1904362. Epub 2019 Dec 12.

Department of Materials Science and Engineering, University of Washington, Seattle, WA, 98195, USA.

Graphene quantum dots (GQDs) are carbon-based, nanoscale particles that exhibit excellent chemical, physical, and biological properties that allow them to excel in a wide range of applications in nanomedicine. The unique electronic structure of GQDs confers functional attributes onto these nanomaterials such as strong and tunable photoluminescence for use in fluorescence bioimaging and biosensing, a high loading capacity of aromatic compounds for small-molecule drug delivery, and the ability to absorb incident radiation for use in the cancer-killing techniques of photothermal and photodynamic therapy. Recent advances in the development of GQDs as novel, multifunctional biomaterials are presented with a focus on their physicochemical, electronic, magnetic, and biological properties, along with a discussion of technical progress in the synthesis of GQDs. Progress toward the application of GQDs in bioimaging, biosensing, and therapy is reviewed, along with a discussion of the current limitations and future directions of this exciting material.
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http://dx.doi.org/10.1002/adma.201904362DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7289657PMC
June 2021
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