Publications by authors named "Sen Chen"

138 Publications

C Solid-State NMR Analysis of the Chemical Structure in Petroleum Coke during Idealized In Situ Combustion Conditions.

ACS Omega 2021 Jun 7;6(23):15479-15485. Epub 2021 Jun 7.

Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.

Petroleum cokes with different chemical structures and oxygen-containing functional groups were obtained from two kinds of naphthenic- and paraffin-base crude oils by simulating an in situ combustion (ISC) process with the same reaction atmosphere and different reaction temperatures. C wide-cavity solid-state nuclear magnetic resonance (C NMR) spectroscopy was used to identify and investigate the oxygen-containing functional groups of petroleum cokes obtained under different compositions and reaction temperatures. This study demonstrated that with the increase of coking temperature, the content of alkyl side chain and active oxygen-containing functional groups in naphthenic-base crude coke decreased obviously, while the content of aromatic carbon increased. The C NMR analysis of the two kinds of petroleum cokes obtained at 500 °C further revealed that the paraffin-base petroleum coke retained a high content of oxygen- and nitrogen-rich functional groups, while the naphthenic-base petroleum coke had a lower amount of carbonyl groups and oxygen-containing functional groups.
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http://dx.doi.org/10.1021/acsomega.1c02055DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8210435PMC
June 2021

Association between baseline and changes in serum uric acid and incident metabolic syndrome: a nation-wide cohort study and updated meta-analysis.

Nutr Metab (Lond) 2021 Jun 9;18(1):59. Epub 2021 Jun 9.

The George Institute for Global Health, Faculty of Medicine, University of New South Wales, Sydney, Australia.

Background: To prospectively examine the associations of baseline serum uric acid (SUA) and SUA changes with incident metabolic syndrome (MetS) and update the evidence through a meta-analysis.

Methods: Our analyses were based on the China Health and Retirement Longitudinal Study from 2011-2012 to 2015-2016. The exposures were baseline SUA and SUA changes, and the outcome was incident MetS assessed in 2015-2016. Logistic regression was used to estimate odds ratios (ORs) and 95% confidence intervals (CIs). A meta-analysis was conducted to synthesize evidence from all cohort studies on the same topic.

Results: Of 3779 participants (47.2% men; mean age: 59.5 years) without MetS, 452 participants developed MetS after a follow-up of 4 years. Compared to the lowest quartiles, the adjusted ORs (95% CIs) for MetS were 1.08 (0.77-1.50), 1.32 (0.95-1.82), and 1.55 (1.12-2.16) for three higher quartiles of baseline SUA, and 1.23 (0.89-1.71), 1.39 (1.00-1.93), and 1.89 (1.38-2.58) for three higher quartiles of SUA changes. Each increment of 1 mg/dL of baseline SUA level was associated with 19% higher odds of MetS (adjusted OR 1.19; 95% CI 1.07-1.33). In the meta-analysis of 24 cohort studies among 140,913 participants, the pooled relative risk (95% CI) was 1.32 (1.25-1.40) for the highest versus lowest SUA category, and 1.15 (1.09-1.21) for each 1 mg/dL increase in the SUA level.

Conclusions: Both baseline SUA and longitudinal SUA changes were positively associated with risk of MetS among middle-aged and elderly Chinese, which was supported by findings from a comprehensive meta-analysis across multiple populations. SUA levels might need to be monitored closely for subsequent risk of MetS in clinical practice.
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http://dx.doi.org/10.1186/s12986-021-00584-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8191036PMC
June 2021

Identification of Novel Compound Heterozygous Mutations in Two Chinese Families with Autosomal Recessive Nonsyndromic Hearing Loss.

Neural Plast 2021 14;2021:9957712. Epub 2021 May 14.

Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.

Congenital deafness is one of the most common causes of disability in humans, and more than half of cases are caused by genetic factors. Mutations of the gene are the third most common cause of hereditary hearing loss. Using next-generation sequencing combined with auditory tests, two novel compound heterozygous variants c.2802_2812del/c.5681T>C and c.5681T>C/c.6340G>A in the gene were identified in probands from two irrelevant Chinese families. Auditory phenotypes of the probands are consistent with the previously reported for recessive variants in the gene. The two novel variants, c.2802_2812del and c.5681T>C, were identified as deleterious mutations by bioinformatics analysis. Our findings extend the gene mutation spectrum and provide more information for rapid and precise molecular diagnosis of congenital deafness.
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http://dx.doi.org/10.1155/2021/9957712DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8140830PMC
May 2021

FOXG1 promotes aging inner ear hair cell survival through activation of the autophagy pathway.

Autophagy 2021 May 19:1-22. Epub 2021 May 19.

Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Presbycusis is the cumulative effect of aging on hearing. Recent studies have shown that common mitochondrial gene deletions are closely related to deafness caused by degenerative changes in the auditory system, and some of these nuclear factors are proposed to participate in the regulation of mitochondrial function. However, the detailed mechanisms involved in age-related degeneration of the auditory systems have not yet been fully elucidated. In this study, we found that FOXG1 plays an important role in the auditory degeneration process through regulation of macroautophagy/autophagy. Inhibition of FOXG1 decreased the autophagy activity and led to the accumulation of reactive oxygen species and subsequent apoptosis of cochlear hair cells. Recent clinical studies have found that aspirin plays important roles in the prevention and treatment of various diseases by regulating autophagy and mitochondria function. In this study, we found that aspirin increased the expression of FOXG1, which further activated autophagy and reduced the production of reactive oxygen species and inhibited apoptosis, and thus promoted the survival of mimetic aging HCs and HC-like OC-1 cells. This study demonstrates the regulatory function of the FOXG1 transcription factor through the autophagy pathway during hair cell degeneration in presbycusis, and it provides a new molecular approach for the treatment of age-related hearing loss.: AHL: age-related hearing loss; baf: bafilomycin A1; CD: common deletion; D-gal: D-galactose; GO: glucose oxidase; HC: hair cells; mtDNA: mitochondrial DNA; RAP: rapamycin; ROS: reactive oxygen species; TMRE: tetramethylrhodamine, ethyl ester.
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http://dx.doi.org/10.1080/15548627.2021.1916194DOI Listing
May 2021

Scrotal septal flap and two-stage operation for complex hypospadias: A retrospective study.

World J Clin Cases 2021 May;9(13):2983-2993

The 2 Department of Plastic Surgery, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100144, China.

Background: Complex hypospadias is a surgical challenge.

Aim: To present the long-term outcomes of two-stage repair of complex hypospadias using a scrotal septal flap.

Methods: This was a retrospective study of patients with complex hypospadias who were operated on between January 1, 2001, and January 1, 2019, at a single hospital using a scrotal septal flap (two-stage surgery) or prepuce flap (one-stage surgery; control group). In the scrotal group, the urethra was first repaired using oral mucosa; in the second stage, a scrotal septal flap was used as a second imper-meable layer. Maximal/average urinary flow rates after surgery were compared. All patients were followed for ≥ 6 mo (range: 6-96 mo).

Results: Ninety-seven patients were included (46 in the scrotal group and 51 in the prepuce group). The maximal urinary flow rate was 15.4 ± 2.1 mL/s in the scrotal group and 14.3 ± 3.0 mL/s in the control group ( = 0.035). The average urinary flow rate was 8.4 ± 2.3 mL/s in the scrotal group and 7.5 ± 1.5 mL/s in the control group ( = 0.019). The proportion of patients achieving good therapeutic effects was higher in the scrotal group than in the control group [24 (52.2%) 16 (31.4%), = 0.042; 34 (73.9%) 25 (49.0%), = 0.014]. The scrotal flap two-stage surgery was independently associated with a higher maximal urinary flow rate (OR = 2.416, 95%CI: 1.026-5.689, = 0.044) and with a higher average flow rate (OR = 2.484, 95%CI: 1.054-5.854, = 0.038).

Conclusion: In complex hypospadias, a scrotal septal flap could be a versatile and reliable option for resurfacing the penis.
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http://dx.doi.org/10.12998/wjcc.v9.i13.2983DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8080759PMC
May 2021

N-Acetylcysteine Combined With Dexamethasone Treatment Improves Sudden Sensorineural Hearing Loss and Attenuates Hair Cell Death Caused by ROS Stress.

Front Cell Dev Biol 2021 18;9:659486. Epub 2021 Mar 18.

Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Sudden sensorineural hearing loss (SSNHL) is a common emergency in the world. Increasing evidence of imbalance of oxidant-antioxidant were found in SSNHL patients. Steroids combined with antioxidants may be a potential strategy for the treatment of SSNHL. In cochlear explant experiment, we found that N-acetylcysteine (NAC) combined with dexamethasone can effectively protect hair cells from oxidative stress when they were both at ineffective concentrations alone. A clinic trial was designed to explore whether oral NAC combined with intratympanic dexamethasone (ITD) as a salvage treatment has a better therapeutic effect. 41 patients with SSNHL were randomized to two groups. 23 patients in control group received ITD therapy alone, while 18 patient s in NAC group were treated with oral NAC and ITD. The patients were followed-up on day 1st (initiation of treatment) and day 14th. Overall, there was no statistical difference in final pure-tone threshold average (PTA) improvement between those two groups. However, a significant hearing gain at 8,000 Hz was observed in NAC group. Moreover, the hearing recovery rates of NAC group is much higher than that in control group. These results demonstrated that oral NAC in combination with ITD therapy is a more effective therapy for SSNHL than ITD alone.
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http://dx.doi.org/10.3389/fcell.2021.659486DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8014036PMC
March 2021

[The value of genetic diagnosis of deafness in evaluating the prognosis of cochlear implantation].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2021 Mar;35(3):274-281

Congenital deafness is known as the most common birth defect, and most sufferers from it manifest sensorineural hearing loss (SNHL), with hereditary factors responsible for approximately 60% of the cases of deafness. At present, cochlear implantation (CI) is regarded as the most mature and effective solution to treating severe and extremely severe SNHL. However, the outcome of implantation varies due to different genetic factors. With whole genome sequencing advancing, more deafness mutant genes and their types have been identified, which is conducive to clarifying the efficacy of CI in the patients with different mutations for clinical practice. This paper is aimed to summarize the different effects of CI on hereditary deafness and the potential mechanism discovered in recent years, and to clarify the role played by the genetic diagnosis of deafness in evaluating the efficacy of cochlear implantation.
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http://dx.doi.org/10.13201/j.issn.2096-7993.2021.03.020DOI Listing
March 2021

[Nonsyndromic deafness due to compound heterozygous mutation of the gene].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2021 Mar;35(3):229-233;237

Department of Otorhinolaryngology Head and Neck Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,430022,China.

To identify the pathogenic gene mutation of two patients with non-syndromic deafness(NSHL). Two patient with NSHL and their parents were selected in the research object. Each participant provided 3-5 mL of peripheral venous blood, which was used to establish a DNA library. Next generation sequencing was used to detect the sequence of the patient's genome, and the sequencing results were compared with the human genome sequence (GRCh)37/hg19. Sanger sequencing was used to verify the parents' genome sequence. Finally the patient's pathogenic gene mutation was confirmed.Amino acid conservatism and single nucleotide polymorphisms of the mutant sites were analyzed using a variety of databases and software. The mutation was located to gene in the chromosomal location 10q21-q22. Complex heterozygous mutations consist of c. 1343T>C and c. 7991_7993delTCA. Parents are heterozygous carriers of a single mutation. The next generation sequencing technology were used to screen the pathogenic gene mutation of inherited deafness. Combined with the genetic sequencing results of parents, the specific pathogenic gene mutation of deafness patients can be identified. While the pathogenicity of complex heterozygous mutation were explained by various pathogenicity analysis methods.
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http://dx.doi.org/10.13201/j.issn.2096-7993.2021.03.008DOI Listing
March 2021

Liquid Metal Foaming via Decomposition Agents.

ACS Appl Mater Interfaces 2021 Apr 31;13(14):17093-17103. Epub 2021 Mar 31.

Beijing Key Lab of CryoBiomedical Engineering and Key Lab of Cryogenics, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

As an emerging functional material, the liquid metal has demonstrated its encouraging potential in several areas with practical trials, while its global uniformity including high density and limited macroscopic interface might become a barrier for some tough application scenarios. Here, we proposed the concept of liquid metal foaming via decomposition agents, aiming to develop a generalized way to make porous foam metallic fluid, which would pave the way in achieving more structured features and adaptability of liquid metals. By introducing a greenness strategy with the help of an ecofriendly foaming agent, we realized a series of designed targeted liquid metal foams (LMFs). Compared with common liquid metals, LMFs possess many excellent properties, such as abundant interfaces, tunable conductivity, and adjustable stiffness, due to the controllable regulation of their porous structure. According to these unique characteristics, diversified values of LMFs were obtained. Benefiting from the naturally enriched interface in LMFs, the hydrogen evolution of LMFs in neutral deionized water was more efficient and more productive. Additionally, the compact LMF-air battery with high performance was originally manufactured. Moreover, the tunable LMF-enabled four-dimensional (4D) electromagnetic shielding materials possess excellent shielding performance. This material could open up broad vistas for the application of LMs.
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http://dx.doi.org/10.1021/acsami.1c01731DOI Listing
April 2021

A New Modified Labiaplasty Combined with Wedge De-Epithelialization on the Medial Side and Edge Resection.

Aesthetic Plast Surg 2021 Aug 8;45(4):1869-1876. Epub 2021 Mar 8.

Plastic Surgery Hospital, Peking Union Medical College, Chinese Academy of Medical Science, 33 Badachu road, Shijingshan District, Beijing, 100144, China.

Background: Labiaplasty (labia minora reduction) has been increasingly requested in recent years. The surgical procedures previously reported in the literature may have some complications that affect patient satisfaction.

Methods: Patients who underwent a new bilateral labiaplasty procedure that combined wedge de-epithelialization on the medial side with edge resection on the lateral side were retrospectively reviewed. Satisfaction rates were assessed, and questionnaires, namely the Female Sexual Function Index (FSFI) and Genital Appearance Satisfaction (GAS) scale, were distributed and analyzed. The data from the study group were compared with data from cases who underwent traditional labiaplasty with wedge resection (control group).

Results: From October 2015 to February 2020, fifty-one women underwent modified labiaplasty, while 26 women underwent traditional labiaplasty. A total of 94.1% (48/51) of patients in the modified group were satisfied with the genital appearance. Compared with the 96.2% (25/26) satisfaction rate in the control group, the difference was not statistically significant. A total of 43/55 valid questionnaires were returned, including 29 in the modified labiaplasty group and 14 in the control group. There was a significant improvement in the postoperative GAS scale compared to the preoperative scale in both groups (P<0.05). The new modified method had a significant FSFI improvement after the operation compared with preoperative scores (P<0.05), while the FSFI improvement was not significant in the control group (P>0.05). There was no significant difference in the FSFI score improvement, postoperative GAS score or postoperative FSFI between the two groups (P>0.05).

Conclusion: This new modified labiaplasty is a satisfying and safe method with low risks, and it may result in better sexual sensitivity for the patients LEVEL OF EVIDENCE IV: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine Ratings, please refer to Table of Contents or online Instructions to Authors www.springer.com/00266 .
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http://dx.doi.org/10.1007/s00266-021-02137-2DOI Listing
August 2021

CCDC154 Mutant Caused Abnormal Remodeling of the Otic Capsule and Hearing Loss in Mice.

Front Cell Dev Biol 2021 4;9:637011. Epub 2021 Feb 4.

Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Osteopetrosis is a rare inherited bone disease characterized by dysfunction of osteoclasts, causing impaired bone resorption and remodeling, which ultimately leads to increased bone mass and density. Hearing loss is one of the most common complications of osteopetrosis. However, the etiology and pathogenesis of auditory damage still need to be explored. In this study, we found that a spontaneous mutation of coiled-coil domain-containing 154 (CCDC154) gene, a new osteopetrosis-related gene, induced congenital deafness in mice. Homozygous mutant mice showed moderate to severe hearing loss, while heterozygous or wild-type (WT) littermates displayed normal hearing. Pathological observation showed that abnormal bony remodeling of the otic capsule, characterized by increased vascularization and multiple cavitary lesions, was found in homozygous mutant mice. Normal structure of the organ of Corti and no substantial hair cell or spiral ganglion neuron loss was observed in homozygous mutant mice. Our results indicate that mutation of the osteopetrosis-related gene CCDC154 can induce syndromic hereditary deafness in mice. Bony remodeling disorders of the auditory ossicles and otic capsule are involved in the hearing loss caused by CDCC154 mutation.
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http://dx.doi.org/10.3389/fcell.2021.637011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7889813PMC
February 2021

Pervasive liquid metal printed electronics: From concept incubation to industry.

Authors:
Sen Chen Jing Liu

iScience 2021 Jan 5;24(1):102026. Epub 2021 Jan 5.

Beijing Key Lab of Cryo-Biomedical Engineering, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

Electronic devices play vital role in modern civilization. Compared to conventional electronic manufacturing, the recently emerging liquid metal printed electronics (LMPE) is opening many extraordinary opportunities, such as large-area printing, pervasive adaptability, flexibility for personal use, low cost, high performance, and environmental friendliness. More uniquely, liquid metal printing allows customize electronic products on demand to fabricate electronics spanning from 2D plane surface to 3D structure and on any desired substrates. This deems it to reshape modern electronics and integrated circuits field. So far, a variety of technological breakthroughs in this new generation electronic engineering area have been made in the process of developing various liquid metal functional inks, printing machines and applications, which significantly stimulate the quick incubation and formation of a new electronic industry. Clearly, sorting out the major R&D directions and clarifying future challenges is crucial for the large scale industrialization of LMPE. This perspective article is dedicated to briefly outline the representative principles and key technologies lying behind, and illustrate the milestone products and equipment thus invented for the coming LMPE industry. In addition, we evaluate the corresponding industrialization trends and promising roadmap and interpret future prospects for the new era of pervasive electronics when anyone can freely use such a tool to print out himself functional electronic device to fulfill various purposes at anywhere and anytime.
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http://dx.doi.org/10.1016/j.isci.2020.102026DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7814192PMC
January 2021

Local Macrophage-Related Immune Response Is Involved in Cochlear Epithelial Damage in Distinct -Related Hereditary Deafness Models.

Front Cell Dev Biol 2020 11;8:597769. Epub 2021 Jan 11.

Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

The macrophage-related immune response is an important component of the cochlear response to different exogenous stresses, including noise, ototoxic antibiotics, toxins, or viral infection. However, the role of the immune response in hereditary deafness caused by genetic mutations is rarely explored. , encoding connexin 26 (Cx26), is the most common deafness gene of hereditary deafness. In this study, two distinct Cx26-null mouse models were established to investigate the types and underlying mechanisms of immune responses. In a systemic Cx26-null model, macrophage recruitment was observed, associated with extensive cell degeneration of the cochlear epithelium. In a targeted-cell Cx26-null model, knockout of Cx26 was restricted to specific supporting cells (SCs), which led to preferential loss of local outer hair cells (OHCs). This local OHC loss can also induce a macrophage-related immune response. Common inflammatory factors, including TNF-α, IL-1β, Icam-1, Mif, Cx3cr1, Tlr4, Ccl2, and Ccr2, did not change significantly, while mRNA of Cx3cl1 was upregulated. Quantitative immunofluorescence showed that the protein expression of CX3CL1 in Deiters cells, a type of SC coupled with OHCs, increased significantly after OHC death. OHC loss caused the secondary death of spiral ganglion neurons (SGNs), while the remaining SGNs expressed high levels of CX3CL1 with infiltrated macrophages. Taken together, our results indicate that CX3CL1 signaling regulates macrophage recruitment and that enhancement of macrophage antigen-presenting function is associated with cell degeneration in Cx26-null mice.
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http://dx.doi.org/10.3389/fcell.2020.597769DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829512PMC
January 2021

Association between metabolic syndrome and incident chronic kidney disease among Chinese: A nation-wide cohort study and updated meta-analysis.

Diabetes Metab Res Rev 2021 Jan 19. Epub 2021 Jan 19.

Division of Epidemiology, Department of Medicine, Vanderbilt Epidemiology Center, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

Aims: We prospectively examined the relationship between metabolic syndrome (MetS) and incident chronic kidney disease (CKD) among middle-aged and elderly Chinese, and conducted a systematic review and meta-analysis of all cohort studies on this topic.

Materials And Methods: Our research data were derived from the China Health and Retirement Longitudinal Study. Participants (n=5752, age ≥45 years) without CKD (defined as estimated glomerular filtration rate <60 ml/min/1.73m ) at baseline were followed up for 4 years. We applied logistic regressions to examine the association of MetS with incident CKD. In addition, we pooled our effect estimates and those from previous cohort studies in the meta-analysis.

Results: In a 4-years follow-up, 61 (4.27%) developed CKD in participants with MetS versus 102 (2.36%) in participants without MetS. After adjustment for potential confounders, odds ratio for incident CKD was 1.82 [95% confidence interval (95% CI): 1.19-2.78] comparing participants with MetS with those without MetS. There was a linear positive association between the number of MetS components and incident CKD (p for trend <0.001). In the updated meta-analysis of 25 studies among 350,655 participants with 29,368 incident cases of CKD, the pooled relative risk of developing CKD in participants with MetS was 1.34 (95% CI: 1.28-1.39), compared with those without MetS.

Conclusions: Individuals with MetS had higher risk of incident CKD in middle-aged and elderly Chinese adults, which was supported by a comprehensive review of cohort studies from multiple populations. It may be advisable to routinely monitor renal functions among individuals with MetS.
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http://dx.doi.org/10.1002/dmrr.3437DOI Listing
January 2021

Mussel-Inspired Multifunctional Integrated Liquid Metal-Based Magnetic Suspensions with Rheological, Magnetic, Electrical, and Thermal Reinforcement.

ACS Appl Mater Interfaces 2021 Feb 19;13(4):5256-5265. Epub 2021 Jan 19.

Key Laboratory of Cryogenics and Beijing Key Laboratory of Cyro-Biomedical Engineering, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

Magnetic liquid metal is regarded as a promising material due to its integration of fluidic, metallic, and magnetic properties simultaneously. Previously, few methods of fabricating magnetic liquid metal have been proposed. However, either the alloying reaction inside the matrix or the poor performance in electrical and thermal conduction is troublesome in practical applications. Here, inspired by the mussel in nature, polydopamine is introduced to in situ reduce and immobilize silver shells on the surface of iron particles, and then the modified particles mix with liquid metal to prepare liquid metal-based magnetic suspensions (LMMSs). The silver shells can prevent iron particles from alloying with liquid metal and enhance the electrical and thermal conductivities of the LMMS concurrently. Besides, the LMMS thus obtained can keep its magnetism intact for a long period, at least during the 60 days of the test. Compared to directly mixing bare iron particles with liquid metal, the maximum electrical conductivities increase by at least 13.69% and the thermal conductivities increase by almost 4 times in the LMMS. The LMMS also exhibits potential applications in patterning and magnetic manipulation. This work puts forward a new strategy for preparing a LMMS with appealing properties and its broad applications are expected in the future.
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http://dx.doi.org/10.1021/acsami.0c20485DOI Listing
February 2021

LncRNA LOC100912373 modulates PDK1 expression by sponging miR-17-5p to promote the proliferation of fibroblast-like synoviocytes in rheumatoid arthritis.

Am J Transl Res 2020 15;12(12):7709-7723. Epub 2020 Dec 15.

Experimental Center of Clinical Research, The First Affiliated Hospital of Anhui University of Chinese Medicine Hefei, Anhui, China.

Rheumatoid arthritis (RA) is a common autoimmune disease and characterized by chronic inflammation, abnormal synovial cell proliferation, and joint swelling and tenderness, and it causes patients substantial pain. To date, the pathogenesis of RA remains unclear, and specific treatment is still lacking in the clinic. Evidence from previous research indicated that the long noncoding RNA (lncRNA) LOC100912373 is a key lncRNA and involved in RA. However, our understanding of the specific mechanism of lncRNA LOC100912373 in RA development and progression is still in its infancy. In this study, fibroblast-like synoviocytes (FLSs) were cultured by enzyme-dispersed and substrate-attached explant methods. The MTT method, flow cytometry and transmission electron microscopy were used to determine the effect of lncRNA LOC100912373 on FLSs. The expression of key genes such as lncRNA LOC100912373, miR-17-5p, PDK1 and AKT in FLSs was detected by RT-qPCR, immunofluorescence and Western blot. The localization of lncRNA LOC100912373 was determined by fluorescence in situ hybridization. The specific targeting relationship between lncRNA LOC100912373 and miR-17-5p/PDK1 was verified by RNA immunoprecipitation and luciferase reporter gene analysis. The results showed that lncRNA LOC100912373 localized in the cytoplasm and was highly expressed in the synovial tissues and FLSs of AA rats. LncRNA LOC100912373 overexpression promoted the proliferation of FLSs. In addition, lncRNA LOC100912373 could bind to miR-17-5p, and the expression of lncRNA LOC100912373 was negatively correlated with miR-17-5p and positively correlated with PDK1/AKT. In conclusion, lncRNA LOC100912373 may upregulate the expression of PDK1 by sponging miR-17-5p, accelerating the phosphorylation of AKT and inducing the proliferation of FLSs, thus promoting the occurrence and development of RA.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7791483PMC
December 2020

Facial Animation With Free Functional Gracilis Transfer Innervated by the Cross-Facial Nerve Graft.

J Craniofac Surg 2021 Jul-Aug 01;32(5):1754-1757

The 2nd Department of Plastic Surgery, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: The treatment of long-standing facial paralysis has always been a challenge for plastic surgery. The purpose of this study was to demonstrate that the free functional gracilis transfer innervated by the cross-facial nerve graft (CFNG) is still an ideal option, even though there are many new surgical options available.

Methods: A retrospective survey was made on 12 patients who received free functional gracilis transfer innervated by the CFNG. A modified version of the House-Brackmann scale was used to evaluate the movement of the corners of mouth after surgery. Patients were also asked about their satisfaction with the operation. In addition, an objective test was performed to assess the postoperative angle improvement by measuring the angle formed between the horizontal line of both corners of the lips and the vertical midline.

Results: All grafts survived well. No severe complication occurred. Three patients received further surgical operations for aesthetic reasons. The movement of the corners of mouth was classified as excellent in 8 cases, good in three cases, and fair in one cases. The static angle and dynamic angle of postoperation improved and the range of dynamic angle improvement was larger than that of static angle.

Conclusion: Free functional gracilis transfer innervated by the CFNG is an ideal technique for facial paralysis. It can effectively improve the facial dynamic of the affected side.
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http://dx.doi.org/10.1097/SCS.0000000000007251DOI Listing
November 2020

Global prevalence of congenital heart disease in school-age children: a meta-analysis and systematic review.

BMC Cardiovasc Disord 2020 11 19;20(1):488. Epub 2020 Nov 19.

Division of Cardiovascular Sciences, University of Manchester, AV Hill Building, Manchester, M13 9PT, UK.

Background: Congenital heart disease (CHD) is the commonest birth defect. Studies estimating the prevalence of CHD in school-age children could therefore contribute to quantifying unmet health needs for diagnosis and treatment, particularly in lower-income countries. Data at school age are considerably sparser, and individual studies have generally been of small size. We conducted a literature-based meta-analysis to investigate global trends over a 40-year period.

Methods And Results: Studies reporting on CHD prevalence in school-age children (4-18 years old) from 1970 to 2017 were identified from PubMed, EMBASE, Web of Science and Google Scholar. According to the inclusion criteria, 42 studies including 2,638,475 children, reporting the prevalence of unrepaired CHDs (both pre-school diagnoses and first-time school-age diagnoses), and nine studies including 395,571 children, specifically reporting the prevalence of CHD first diagnosed at school ages, were included. Data were combined using random-effects models. The prevalence of unrepaired CHD in school children during the entire period of study was 3.809 (95% confidence intervals 3.075-4.621)/1000. A lower proportion of male than female school children had unrepaired CHD (OR = 0.84 [95% CI 0.74-0.95]; p = 0.001). Between 1970-1974 and 1995-1999, there was no significant change in the prevalence of unrepaired CHD at school age; subsequently there was an approximately 2.5-fold increase from 1.985 (95% CI 1.074-3.173)/1000 in 1995-1999 to 4.832 (95% CI 3.425-6.480)/1000 in 2010-2014, (p = 0.009). Among all CHD conditions, atrial septal defects and ventricular septal defects chiefly accounted for this increasing trend. The summarised prevalence (1970-2017) of CHD diagnoses first made in childhood was 1.384 (0.955, 1.891)/1000; during this time there was a fall from 2.050 [1.362, 2.877]/1000 pre-1995 to 0.848 [0.626, 1.104]/1000 in 1995-2014 (p = 0.04).

Conclusions: Globally, these data show an increased prevalence of CHD (mainly mild CHD conditions) recognised at birth/infancy or early childhood, but remaining unrepaired at school-age. In parallel there has been a decrease of first-time CHD diagnoses in school-age children. These together imply a favourable shift of CHD recognition time to earlier in the life course. Despite this, substantial inequalities between higher and lower income countries remain. Increased healthcare resources for people born with CHD, particularly in poorer countries, are required.
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http://dx.doi.org/10.1186/s12872-020-01781-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7678306PMC
November 2020

High performance liquid metal thermal interface materials.

Nanotechnology 2021 Feb;32(9):092001

Beijing Key Lab of Cryo-Biomedical Engineering, Key Lab of Cryogenics, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, People's Republic of China.

Conventional thermal interface materials (TIMs) as widely used in thermal management area is inherently limited by their relatively low thermal conductivity. From an alternative, the newly emerging liquid metal based thermal interface materials (LM-TIMs) open a rather promising way, which can pronouncedly improve the thermal contact resistance and offers tremendous opportunities for making powerful thermal management materials. The LM-TIMs thus prepared exhibits superior thermal conductivity over many conventional TIMs which guarantees its significant application prospect. And the nanoparticles mediated or tuned liquid metal further enable ever conductive LM-TIMs which suggests the ultimate goal of thermal management. In this review, a systematic interpretation on the basic features of LM-TIMs was presented. Representative exploration and progress on LM-TIMs were summarized. Typical approaches toward nanotechnology enhanced high performance LM-TIMs were illustrated. The perspect of this new generation thermal management material were outlined. Some involved challenges were raised. This work is expected to provide a guide line for future research in this field.
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http://dx.doi.org/10.1088/1361-6528/abcbc2DOI Listing
February 2021

Influence of self-management exercise intervention on the cancer related fatigue severity and self-management efficacy of patients with non-small cell lung cancer after operation.

J Pak Med Assoc 2020 Sep;70 [Special Issue](9):88-93

Department of Clinical Skills Training Center, Hubei University of Medicine, shiyan, China.

Objective: To investigate the influence of self-management exercise intervention on postoperative cancer-related fatigue severity and self-management efficacy of patients with non-small cell lung cancer.

Methods: Seventy patients with non-small cell lung cancer were selected in Pidu district people's hospital from January 2018 to January 2019 and randomised into 2 groups with the random number table, including control group (35 cases) and experimental group (35 cases). The control group received conventional intervention, while the experimental group was intervened by self-management exercise based on the conventional intervention. The baseline clinical characteristics, simple fatigue scale scores, self-efficacy indicators, and physical and mental health scores of 2 groups were compared.

Results: The simple fatigue scale score of both groups were measured six weeks after surgery. The experimental group was significantly lower than that of control group (P<0.05). The self-efficacy scores of continuous walking for 30 minutes six weeks after the operation in both groups continuously increased with time and the result of the experimental group was significantly higher than that of control group (P<0.05). Similarly the postoperative balance ability self-efficacy scores of both groups were on the rise with time but the score of experimental group was significantly higher than that of control group after 6 weeks of surgical treatment (P<0.05). The postoperative health scores of both groups improved with time and the physical and mental health scores of experimental group were significantly higher than those of control group after 6 weeks of surgical treatment (P<0.05).

Conclusions: Self-management exercise intervention can effectively alleviate postoperative cancer-related fatigue of patients with non-small cell lung cancer, enhance self-management efficiency and contribute to improving the status of body functions.
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September 2020

Motion behaviour of ellipsoidal granular system under vertical vibration and airflow.

Soft Matter 2020 Oct;16(41):9559-9567

School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, China and Beijing Engineering Research Centre of Energy Saving and Environmental Protection, Beijing, 100083, China.

We studied the motion behaviour of ellipsoid particles under vertical vibration and airflow. Three typical convection patterns were observed when submitted to vertical vibration with frequency (f) from 20 Hz to 80 Hz and dimensionless vibration acceleration (Γ) from one to six. We studied the effects of f and Γ on the change of convection patterns. We quantitatively studied the effects of f, Γ, airflow direction, airflow velocity, and particle shape on the convection area and intensity using the area fraction λ and average velocity vz characterizing the convection area and intensity, respectively. Results showed that the convection first occured occurred in the upper part of the granular system. Increasing f and A can both increase the convection area and strengthen the convection intensity. A had a greater influence than f at the same Γ. The wheat particles were more likely to enter the global convection state under the action of the airflow in the opposite direction of gravity. The maximum convection intensity of wheat particles under the airflow in the opposite direction of gravity was approximately 30-35% of the value measured under the airflow along the direction of gravity. The convection area and maximum convection intensity of the spherical particles were approximately 85% and 93% of the measured values for the ellipsoidal particles, respectively. We also analysed the effects of f, Γ, airflow direction, airflow velocity, and particle shape on the convection area on the basis of energy dissipation.
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http://dx.doi.org/10.1039/d0sm01129kDOI Listing
October 2020

A Novel Spontaneous Mutation of the Gene Associated with Waardenburg Syndrome Type II.

Neural Plast 2020 28;2020:9260807. Epub 2020 Aug 28.

Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.

Waardenburg syndrome (WS), also known as auditory-pigmentary syndrome, is the most common cause of syndromic hearing loss. It is responsible for 2-5% of congenital deafness. WS is classified into four types depending on the clinical phenotypes. Currently, pathogenic mutation of , , , , , or can cause corresponding types of WS. Among them, mutation is responsible for approximately 15% of type II WS or 50% of type IV WS. We report the case of a proband in a Chinese family who was diagnosed with WS type II. Whole exome sequencing (WES) of the proband detected a novel heterozygous spontaneous mutation: c.246delC. According to analysis based on nucleic acid and amino acid sequences, this mutation may produce a truncated protein, with loss of the HMG structure domain. Therefore, this truncated protein may fail to activate the expression of the gene, which regulates melanocytic development and plays a key role in WS. Our finding expands the database of mutations associated with WS and provides more information regarding the molecular mechanism of WS.
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http://dx.doi.org/10.1155/2020/9260807DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7474791PMC
August 2020

Chromatin binding of FOXA1 is promoted by LSD1-mediated demethylation in prostate cancer.

Nat Genet 2020 10 31;52(10):1011-1017. Epub 2020 Aug 31.

Center for Personalized Cancer Therapy, University of Massachusetts Boston, Boston, MA, USA.

FOXA1 functions as a pioneer transcription factor by facilitating the access to chromatin for steroid hormone receptors, such as androgen receptor and estrogen receptor, but mechanisms regulating its binding to chromatin remain elusive. LSD1 (KDM1A) acts as a transcriptional repressor by demethylating mono/dimethylated histone H3 lysine 4 (H3K4me1/2), but also acts as a steroid hormone receptor coactivator through mechanisms that are unclear. Here we show, in prostate cancer cells, that LSD1 associates with FOXA1 and active enhancer markers, and that LSD1 inhibition globally disrupts FOXA1 chromatin binding. Mechanistically, we demonstrate that LSD1 positively regulates FOXA1 binding by demethylating lysine 270, adjacent to the wing2 region of the FOXA1 DNA-binding domain. Acting through FOXA1, LSD1 inhibition broadly disrupted androgen-receptor binding and its transcriptional output, and dramatically decreased prostate cancer growth alone and in synergy with androgen-receptor antagonist treatment in vivo. These mechanistic insights suggest new therapeutic strategies in steroid-driven cancers.
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http://dx.doi.org/10.1038/s41588-020-0681-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7541538PMC
October 2020

Low-Temperature Triggered Shape Transformation of Liquid Metal Microdroplets.

ACS Appl Mater Interfaces 2020 Aug 12;12(34):38386-38396. Epub 2020 Aug 12.

Department of Vehicle Engineering College of Engineering China Agricultural University Beijing 100083, P. R. China.

Shape transformable materials that can respond to external environments have attracted widespread interest over the fields of soft robotics, flexible electronics, and tissue engineering. Among stimuli-responsive materials, liquid metals exhibit rather unique characteristics of versatile morphological changes upon diverse stimuli, including chemicals, electrical field, and mechanical force, etc. Herein, a superfast (few milliseconds), large-scaled (13.8% deformation increase), and fierce (cracks formation) transformation of liquid metal microdroplets (LMMs) with strong impulse expanded force due to liquid-solid phase transition in a dual fluid system composed of LMMs and aqueous solution is reported. When subject to low-temperature stimulus, LMM would transform from ellipsoidal shape to amorphous shape induced by thermal stress, driving the shape morphing. Furthermore, the phase changes of LMMs as well as the formation of surrounding ice crystals are proven to be responsible for this phenomenal behavior. The densification of ice crystals is demonstrated to play a significant role in the transformable behavior. In particular, these nonconductive LMMs in aqueous solutions are discovered to turn into conducive materials with an impedance change of about 10 times. The present discovery is of fundamental and practical significance, and would open new venues in fields such as fluid mechanics, thermal science, flexible electronics, biomedicine, and so forth.
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http://dx.doi.org/10.1021/acsami.0c10409DOI Listing
August 2020

Preclinical evidence that MNK/eIF4E inhibition by cercosporamide enhances the response to antiangiogenic TKI and mTOR inhibitor in renal cell carcinoma.

Biochem Biophys Res Commun 2020 09 30;530(1):142-148. Epub 2020 Jul 30.

Department of Oncology, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyang, Hubei Province, China. Electronic address:

Eukaryotic translation initiation factor 4E (eIF4E) is deregulated in patients with renal cell carcinoma (RCC) and associated with poor prognosis, and is activated and regulated by Mnk kinases. In this study, we investigated the anti-RCC potential of a unique Mnk inhibitor cercosporamide. We showed that cercosporamide is active against RCC cells via suppressing growth, survival and migration. Combination indices value indicated that the combination of cercosporamide with sunitinib or temsirolimus are synergistic in RCC. In two independent RCC xenograft mouse models, complete tumor growth arrest or reverse was observed throughout the duration of drug treatment in the combination of cercosporamide with sunitinib or temsirolimus groups. Of note, cercosporamide inhibited RCC angiogenesis via negatively regulating a number of RCC endothelial cellular events including morphogenesis, migration, growth and survival. Mechanistically, we found that cercosporamide suppressed pro-angiogenic factors VEGF and HIFα, inhibited EMT and reduced pro-survival and cell cycle proteins; and furthermore this was attributed to cercosporamide's ability in inhibiting eIF4E. This work demonstrates the anti-RCC activity of cercosporamide through targeting both RCC tumor cells and angiogenesis, and provides the first preclinical proof-of-concept of evidence of Mnk inhibition for RCC treatment.
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http://dx.doi.org/10.1016/j.bbrc.2020.06.133DOI Listing
September 2020

Staged Buccal Mucosa Graft Urethroplasty for Proximal Hypospadias in Children: A Short- to Mid-Term Follow-Up Retrospective Study.

Eur J Pediatr Surg 2020 Aug 20. Epub 2020 Aug 20.

The 2nd Department of Plastic Surgery, Chinese Academy of Medical Sciences & Peking Union Medical College Plastic Surgery Hospital and Institute, Shijingshan District, China.

Introduction:  The aim of this study is to report our experience with modified staged buccal mucosa graft urethroplasty for the repair of proximal hypospadias in children and adolescents.

Materials And Methods:  A total of 183 patients were treated at the Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College between 2010 and 2019. The patients were grouped according to age: (1) within 1.5-year-old group, (2) prepuberty group (Tanner stage 1), and (3) puberty group.

Results:  In total, 153 patients were included in this study. Thirty-six patients had complications: seven after stage one; 29 after stage two; one patient had two complications. Maximum flow rates were 11.80 ± 1.46 mL/s in the 1.5-year-old group, 13.24 ± 2.61 mL/s in the prepuberty group, and 13.60 ± 2.20 mL/s in the puberty group ( = 0.199). Average flow rates were 6.86 ± 1.37, 7.94 ± 1.74, and 7.88 ± 1.22 mL/s, respectively ( = 0.203). The optimal hypospadias objective scoring evaluation score of 16 was seen in 117 patients (76%), the score of 15 in 23 patients (15%), 14 in 10 (7%), and 13 in 3 patients (2%). Patients with an uncomplicated treatment (no complication) had a higher clinical outcome than patients with a complication (15.8 ± 0.53 vs. 15.3 ± 0.97, with or without complication,  = 0.000). Multivariable analyses showed that previous treatment was closely related to the complication rate ( = 0.016).

Conclusion:  The modified procedure allows for two-stage repair of proximal hypospadias with good results with a low complication rate and good functional results. Delaying operation did not increase complication rates in our research.
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http://dx.doi.org/10.1055/s-0040-1715477DOI Listing
August 2020

The Use of Higher Proportions of Platelet-Rich Plasma to Enrich Microfat Has Negative Effects: A Preclinical Study.

Plast Reconstr Surg 2020 08;146(2):228e-229e

Plastic Surgery Hospital, Chinese Academy of Medical Sciences and, Peking Union Medical College, Beijing, People's Republic of China.

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http://dx.doi.org/10.1097/PRS.0000000000006991DOI Listing
August 2020

Machine learning of serum metabolic patterns encodes early-stage lung adenocarcinoma.

Nat Commun 2020 07 16;11(1):3556. Epub 2020 Jul 16.

State Key Laboratory for Oncogenes and Related Genes, School of Biomedical Engineering, Shanghai Jiao Tong University, 200030, Shanghai, P. R. China.

Early cancer detection greatly increases the chances for successful treatment, but available diagnostics for some tumours, including lung adenocarcinoma (LA), are limited. An ideal early-stage diagnosis of LA for large-scale clinical use must address quick detection, low invasiveness, and high performance. Here, we conduct machine learning of serum metabolic patterns to detect early-stage LA. We extract direct metabolic patterns by the optimized ferric particle-assisted laser desorption/ionization mass spectrometry within 1 s using only 50 nL of serum. We define a metabolic range of 100-400 Da with 143 m/z features. We diagnose early-stage LA with sensitivity~70-90% and specificity~90-93% through the sparse regression machine learning of patterns. We identify a biomarker panel of seven metabolites and relevant pathways to distinguish early-stage LA from controls (p < 0.05). Our approach advances the design of metabolic analysis for early cancer detection and holds promise as an efficient test for low-cost rollout to clinics.
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http://dx.doi.org/10.1038/s41467-020-17347-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7366718PMC
July 2020

Mechanical Signals Induce Dedifferentiation of Mature Adipocytes and Increase the Retention Rate of Fat Grafts.

Plast Reconstr Surg 2020 10;146(4):506e-507e

Plastic Surgery Hospital, Chinese Academy of Medical Sciences and, Peking Union Medical College, Beijing, People's Republic of China.

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http://dx.doi.org/10.1097/PRS.0000000000007206DOI Listing
October 2020
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