Mr Sawan Kumar Jha, M.Sc. - University of Helsinki - Graduate student

Mr Sawan Kumar Jha

M.Sc.

University of Helsinki

Graduate student

Helsinki | Finland

ORCID logohttps://orcid.org/0000-0003-1898-4928

Mr Sawan Kumar Jha, M.Sc. - University of Helsinki - Graduate student

Mr Sawan Kumar Jha

M.Sc.

Introduction

Primary Affiliation: University of Helsinki - Helsinki , Finland

Experience

Jan 2015
University of Helsinki
Ph.D fellow
Medicine

Publications

7Publications

99Reads

-Profile Views

60PubMed Central Citations

Key molecules in lymphatic development, function, and identification.

Ann Anat 2018 Sep 26;219:25-34. Epub 2018 May 26.

Translational Cancer Biology Research Program, University of Helsinki, Finland; Wihuri Research Institute, Biomedicum Helsinki, Finland. Electronic address:

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.aanat.2018.05.003DOI Listing
September 2018
11 Reads
2.080 Impact Factor

Biology of Vascular Endothelial Growth Factor C in the Morphogenesis of Lymphatic Vessels.

Front Bioeng Biotechnol 2018 12;6. Epub 2018 Feb 12.

Translational Cancer Biology Research Program, University of Helsinki, Helsinki, Finland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fbioe.2018.00007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5816233PMC
February 2018
4 Reads

Efficient activation of the lymphangiogenic growth factor VEGF-C requires the C-terminal domain of VEGF-C and the N-terminal domain of CCBE1.

Sci Rep 2017 07 7;7(1):4916. Epub 2017 Jul 7.

Translational Cancer Biology Research Program, Biomedicum Helsinki, University of Helsinki, PO Box 63, Haartmaninkatu 8, Helsinki, 00014, Finland.

The collagen- and calcium-binding EGF domains 1 (CCBE1) protein is necessary for lymphangiogenesis. Its C-terminal collagen-like domain was shown to be required for the activation of the major lymphangiogenic growth factor VEGF-C (Vascular Endothelial Growth Factor-C) along with the ADAMTS3 (A Disintegrin And Metalloproteinase with Thrombospondin Motifs-3) protease. However, it remained unclear how the N-terminal domain of CCBE1 contributed to lymphangiogenic signaling. Here, we show that efficient activation of VEGF-C requires its C-terminal domain both in vitro and in a transgenic mouse model. The N-terminal EGF-like domain of CCBE1 increased VEGFR-3 signaling by colocalizing pro-VEGF-C with its activating protease to the lymphatic endothelial cell surface. When the ADAMTS3 amounts were limited, proteolytic activation of pro-VEGF-C was supported by the N-terminal domain of CCBE1, but not by its C-terminal domain. A single amino acid substitution in ADAMTS3, identified from a lymphedema patient, was associated with abnormal CCBE1 localization. These results show that CCBE1 promotes VEGFR-3 signaling and lymphangiogenesis by different mechanisms, which are mediated independently by the two domains of CCBE1: by enhancing the cleavage activity of ADAMTS3 and by facilitating the colocalization of VEGF-C and ADAMTS3. These new insights should be valuable in developing new strategies to therapeutically target VEGF-C/VEGFR-3-induced lymphangiogenesis.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-017-04982-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5501841PMC
July 2017
13 Reads
20 Citations
5.078 Impact Factor

Factors regulating the substrate specificity of cytosolic phospholipase A-alpha in vitro.

Biochim Biophys Acta 2016 Nov 1;1861(11):1597-1604. Epub 2016 Jul 1.

Faculty of Medicine, Department of Biochemistry and Developmental Biology, Finland. Electronic address:

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbalip.2016.06.022DOI Listing
November 2016
21 Reads

Identification of selective cytotoxic and synthetic lethal drug responses in triple negative breast cancer cells.

Mol Cancer 2016 05 10;15(1):34. Epub 2016 May 10.

Institute for Molecular Medicine Finland, University of Helsinki, Helsinki, Finland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12943-016-0517-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4862054PMC
May 2016
16 Reads
6 Citations
4.260 Impact Factor

Systematic Mapping of Kinase Addiction Combinations in Breast Cancer Cells by Integrating Drug Sensitivity and Selectivity Profiles.

Chem Biol 2015 Aug 23;22(8):1144-55. Epub 2015 Jul 23.

Institute for Molecular Medicine Finland, FIMM, University of Helsinki, 00014 Helsinki, Finland. Electronic address:

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chembiol.2015.06.021DOI Listing
August 2015
20 Reads
4 Citations
6.645 Impact Factor

CCBE1 enhances lymphangiogenesis via A disintegrin and metalloprotease with thrombospondin motifs-3-mediated vascular endothelial growth factor-C activation.

Circulation 2014 May 19;129(19):1962-71. Epub 2014 Feb 19.

Wihuri Research Institute (M.J., A.A., V.-M.L., R.K., K.A.), Translational Cancer Biology Program (M.J., S.K.J., D.T., A.A., T.H., K.A.), and Department of Biomedicine (M.J.), Biomedicum Helsinki, University of Helsinki, Helsinki, Finland; Biotechnology Programme, Spanish National Cancer Research Centre, Madrid, Spain (S.O.); Hubrecht Institute, Utrecht, The Netherlands (T.K.); and Helsinki University Central Hospital, Helsinki, Finland (K.A.).

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1161/CIRCULATIONAHA.113.002779DOI Listing
May 2014
14 Reads
30 Citations
14.430 Impact Factor

Top co-authors

Michael Jeltsch
Michael Jeltsch

University of Helsinki

5
Leena Karhinen
Leena Karhinen

Institute of Biotechnology

2
Bhagwan Yadav
Bhagwan Yadav

University of Helsinki

2
Tero Aittokallio
Tero Aittokallio

Institute for Molecular Medicine Finland (FIMM)

2
Krister Wennerberg
Krister Wennerberg

University of North Carolina

2
Prson Gautam
Prson Gautam

Wihuri Research Institute and Research Programs Unit

2
Agnieszka Szwajda
Agnieszka Szwajda

University of Helsinki

2
Kari Alitalo
Kari Alitalo

Wihuri Research Institute and Translational Cancer Biology Program

2