Publications by authors named "Satoshi Takeuchi"

230 Publications

Severe hypertriglyceridemia induced by S-1: Subsequent case series of four patients and further review of the literature.

Int J Clin Pharmacol Ther 2021 Sep 10. Epub 2021 Sep 10.

Objective: We previously reported a case where S-1, containing tegafur, gimeracil, and oteracil potassium, induced severe hypertriglyceridemia. After the case, we regularly monitored serum lipid levels and surprisingly observed an additional 4 cases within 1.5 years. We here report the treatment process.

Case Report: At least 3 patients exhibited hyperlipidemia at baseline; in 2 of them, this was caused by previous fluoropyrimidine treatment. One patient experienced grade 4 hypertriglyceridemia, and the other 3 grade 3 hypertriglyceridemia. One patient developed temporary serum triglyceride elevation during the S-1 administration period, and the 3 experienced persistent elevation. The severity of serum triglyceride level worsened with increasing administration and peaked in cycles 2 - 6. Fenofibrate 80 - 160 mg/day and S-1 dose reduction were effective, with some significantly and others gradually decreasing to grade 0 - 1.

Discussion: The summarized clinical features are as follows: (1) Severe hypertriglyceridemia tends to appear after several treatment cycles and worsens with increasing administration. (2) It tends to occur in patients with hyperlipidemia at baseline. (3) Patients previously affected with fluoropyrimidines-induced hypertriglyceridemia can experience S-1 symptoms. (4) In some cases, it might decrease after the S-1 suspension period. (5) Fibrates and S-1 dose reductions were effective. As the final fluoropyrimidine product is fluorouracil, its presence or that of its metabolizing enzymes and the genetic background of the patients might have affected the results. We should be aware of the risk of temporal and asymptomatic occurrence of S-1-induced hypertriglyceridemia for early detection with appropriate treatment.
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http://dx.doi.org/10.5414/CP204091DOI Listing
September 2021

Bilateral suprascapular notches are asymmetrically shaped in a third of the Asian population.

Knee Surg Sports Traumatol Arthrosc 2021 Aug 21. Epub 2021 Aug 21.

Department of Musculoskeletal Sports Medicine, Research and Innovation, Nagoya City University Graduate School of Medical Science, Nagoya, Japan.

Purpose: The side-to-side differences within an individual's suprascapular notch (SSN) and the clinical characteristics of an ossified superior transverse scapular ligament are unclear. Therefore, the morphological asymmetry of the SSN was investigated, and the factors associated with the ossification of the superior transverse scapular ligament were analyzed.

Methods: Two hundred and seventy-six computed tomography images were retrospectively analyzed, which included those of both scapulae of Asian patients (mean age, 62.1 ± 19.1 years; males, 197) with high-energy injuries or respiratory diseases. Variations in the SSN were classified into six types based on Rengachary's classification using reconstructed three-dimensional computed tomography. The group with a type VI SSN (completely ossified superior transverse scapular ligament) in at least one scapula was compared with the other group for age, sex, and chronic comorbidities.

Results: Among 276 patients, 95 (34.4%) had asymmetric SSNs and 15 (5.4%) had type VI SSNs. There were no significant differences in age, sex, or comorbidities between both the groups. However, on comparing age groups, the prevalence of type VI SSN was higher in patients aged > 70 years than in those aged < 70 years. Fifteen patients had type VI SSNs, which were unilateral in 10 patients.

Conclusion: Asymmetric SSNs were observed in a third of the Asian patients. There were variations in SSNs between individuals and also within an individual. In the cases with suprascapular nerve paralysis, the difference in SSN morphology compared to a healthy side should be considered.

Level Of Evidence: III.
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http://dx.doi.org/10.1007/s00167-021-06679-5DOI Listing
August 2021

Excited-State Proton Transfer Dynamics in LSSmOrange Studied by Time-Resolved Impulsive Stimulated Raman Spectroscopy.

J Phys Chem Lett 2021 Aug 2;12(31):7466-7473. Epub 2021 Aug 2.

Molecular Spectroscopy Laboratory, RIKEN, 2-1 Hirosawa, Wako 351-0198, Japan.

LSSmOrange is a fluorescent protein that exhibits a large energy gap between absorption and emission, which makes it a useful tool for multicolor bioimaging. This characteristic of LSSmOrange originates from excited-state proton transfer (ESPT): The neutral chromophore is predominantly present in the ground state while the bright fluorescence is emitted from the anionic excited state after ESPT. Interestingly, it was reported that this ESPT process follows bimodal dynamics, but its origin has not clearly been understood. We investigate ESPT of LSSmOrange using time-resolved impulsive stimulated Raman spectroscopy (TR-ISRS) that provides femtosecond time-resolved Raman spectra. The results indicate that the bimodal ESPT dynamics originates from the structural heterogeneity of the chromophore. Species-associated Raman spectra obtained by spectral analysis based on singular value decomposition (SVD) suggest that and chromophores coexist in the ground state. It is considered that these two forms are photoexcited and undergo ESPT in parallel, resulting in the bimodal dynamics of ESPT in LSSmOrange.
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http://dx.doi.org/10.1021/acs.jpclett.1c01653DOI Listing
August 2021

Superb microvascular imaging (SMI) detects increased vascularity of the torn anterior cruciate ligament.

Knee Surg Sports Traumatol Arthrosc 2021 Jun 13. Epub 2021 Jun 13.

Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, PA, USA.

Purpose: Ultrasound with superb microvascular imaging (SMI) is a novel microvascular imaging technology which may be useful to assess the vascularity of the torn anterior cruciate ligament (ACL) as a potential measure of healing potential following surgery. This study aimed to quantify the vascularity of the torn and intact ACL using ultrasound with SMI.

Methods: 23 patients (mean age ± standard deviation, 27.1 ± 12.8 years), who were diagnosed with an ACL tear with an intact contralateral ACL were enrolled (ACL injury group). Ten healthy volunteers (36.1 ± 4.9 years) who had intact ACLs in both knees were also recruited (ACL healthy controls). The vascularity of the ACL was assessed using SMI within 15 mm from the tibial insertion in both knees. The amount of the vascular signal was assessed using a semi-quantitative grading scale (vascularity grade: grade 0-3) and a quantified ratio of vascularized area with respect to total area of the region of interest (vascularity ratio).

Results: In the ACL injury group, a significantly higher vascularity grade and ratio were observed in the torn ACL (vascularity grade 0-3: 1, 8, 7, and 7 patients, respectively; vascularity ratio: 1.3 ± 1.4%) than the contralateral intact ACL (vascularity grade 0-3: 21, 1, 1, and 0 patients, respectively; vascularity ratio: 0.1 ± 0.5%) (P < 0.001), whereas no significant difference was observed between both ACLs in the ACL healthy control group.

Conclusions: SMI was useful to assess the increased vascularity in torn ACL, which may reflect the potential for, or state of, ACL maturation following reconstruction or repair.

Level Of Evidence: Level III.
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http://dx.doi.org/10.1007/s00167-021-06640-6DOI Listing
June 2021

Skeletal Structure of the Chromophore of Photoactive Yellow Protein in the Excited State Investigated by Ultraviolet Femtosecond Stimulated Raman Spectroscopy.

J Phys Chem B 2021 06 8;125(23):6154-6161. Epub 2021 Jun 8.

Molecular Spectroscopy Laboratory, RIKEN, 2-1 Hirosawa, Wako 351-0198, Japan.

We studied ultrafast structural dynamics of photoactive yellow protein (PYP) using ultraviolet femtosecond stimulated Raman spectroscopy. By employing the Raman pump and probe pulses in the ultraviolet region, resonantly enhanced, rich vibrational features of the excited-state chromophore were observed in the fingerprint region. In contrast to the marked spectral change reported for the excited-state chromophore in solution, in the protein, all of the observed Raman bands in the fingerprint region did not show any noticeable spectral shifts nor band shape changes during the excited-state lifetime of PYP. This indicates that the significant skeletal change does not occur on the chromophore in the excited state of PYP and that the conformation is retained in its lifetime. Based on the femtosecond Raman data of PYP obtained so far, we discuss a comprehensive picture of the excited-state structural dynamics of PYP.
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http://dx.doi.org/10.1021/acs.jpcb.1c02828DOI Listing
June 2021

Dupilumab shows slow, steady effectiveness for intractable prurigo in patients with atopic dermatitis.

J Dermatol 2021 May 20;48(5):638-644. Epub 2021 Mar 20.

Department of Dermatology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.

Prurigo lesions in atopic dermatitis are intractable. This single-center, retrospective study examined dupilumab's clinical effects on intractable prurigo. Twenty adult atopic dermatitis patients (12 with prurigo, eight without) were administrated dupilumab. Its effects on itching and disease severity were examined with Numerical Rating Scale-Itch (NRS-I), Eczema Area and Severity Index (EASI), and Investigator Global Assessment (IGA) scores; body surface areas (BSA); and thymus- and activation-regulated chemokine (TARC), total immunoglobulin (Ig)E, and eosinophil levels. NRS-I scores, EASI scores, TARC levels, and total IgE levels before dupilumab treatment were not statistically different between the prurigo and non-prurigo groups. With dupilumab treatment, NRS-I scores, EASI scores, IGA scores, BSA, TARC levels, and total IgE levels were significantly reduced from baseline in both groups at 1-2 months and onward, but skin symptom improvement in the prurigo group was slower than in the non-prurigo group, as evidenced by significantly higher EASI scores, BSA, and TARC levels at several time points during the 12 months of dupilumab treatment. Prurigo patients were slower in EASI-50 achievement and significantly lower in EASI-90 achievement at 12 months than non-prurigo patients. Adherence to dupilumab was not different, but total equivalent amounts of concomitant therapeutic agents (corticosteroids and tacrolimus) used during dupilumab treatment were significantly higher in the prurigo group (median, 56.2 g/week) than in the non-prurigo group (median, 33.7 g/week). There were 2.2 adverse events per patient on average; ocular complaints were most frequent. Dupilumab was effective in treating intractable prurigo, but despite significantly greater concomitant therapeutic agent use, skin symptom improvement was slower in prurigo patients than in non-prurigo patients.
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http://dx.doi.org/10.1111/1346-8138.15843DOI Listing
May 2021

Mode-Specific Vibrational Analysis of Exciton Delocalization and Structural Dynamics in Conjugated Oligomers.

Angew Chem Int Ed Engl 2021 Jul 6;60(31):16999-17008. Epub 2021 May 6.

Department of Chemistry, Spectroscopy Laboratory for Functional, π-Electronic Systems, Yonsei University, Seoul, 03722, Korea.

Exciton delocalization in organic semiconducting polymers, affected by structures at a molecular level, plays a crucial role in modulating relaxation pathways, such as charge generation and singlet fission, which can boost device efficiency. However, the structural diversity of polymers and broad signals from typical electronic spectroscopy have their limits when it comes to revealing the interplay between local structures and exciton delocalization. To tackle these problems, we apply femtosecond stimulated Raman spectroscopy in archetypical conjugated oligothiophenes with different chain lengths. We observed Raman frequency dispersions of symmetric bond stretching modes and mode-specific kinetics in the S Raman spectra, which underpins the subtle and complex interplay between exciton delocalization and bond length alternation along the conjugation coordinate. Our results provide a more general picture of exciton delocalization in the context of molecular structures for conjugated materials.
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http://dx.doi.org/10.1002/anie.202102168DOI Listing
July 2021

An improved quantitative ultrasonographic technique could assess anterior translation of the glenohumeral joint accurately and reliably.

Knee Surg Sports Traumatol Arthrosc 2021 Aug 8;29(8):2595-2605. Epub 2021 Feb 8.

Orthopaedic Robotics Laboratory, University of Pittsburgh, 300 Technology Drive, Pittsburgh, PA, 15219, USA.

Purpose: Since poor repeatability of the load and shift test using a grading scale has been reported, an objective and quantitative method to assess anterior translation should be established to assess glenohumeral joint function. The purpose of this study was to assess the accuracy and repeatability of the ultrasonographic techniques to quantify anterior translation of the glenohumeral joint.

Methods: Eight fresh-frozen cadaveric shoulders were used. For the standard technique, the ultrasound transducer was positioned on the anterolateral aspect of the shoulder viewing the coracoid process, glenoid, and humeral head. For the revised technique, the transducer was positioned on the anterior aspect of the shoulder, perpendicular to the scapular plane, viewing the conjoint tendon, glenoid, and humeral head. During the load and shift test, the distance between anterior edges of the glenoid and the humeral head was measured. The difference between distances before and after applying an anterior load was calculated as an anterior translation and compared with the anterior translation assessed using a motion tracking system. The repeatability and accuracy of both techniques were analyzed statistically.

Results: Intra- and inter-observer repeatability was good-excellent for both ultrasonographic techniques (ICC, 0.889-0.998). The revised technique achieved a stronger correlation to the anterior translations obtained using the motion tracking system (R = 0.810-0.913, p < 0.001) than the standard technique (R = 0.619-0.806, p < 0.001).

Conclusion: Better accuracy and repeatability was found in the revised technique than the standard technique. The revised technique will be useful to determine the individual laxity and modify the treatment plan and return-to-sports protocol.

Level Of Evidence: III.
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http://dx.doi.org/10.1007/s00167-021-06459-1DOI Listing
August 2021

Preoperative ultrasound predicts the intraoperative diameter of the quadriceps tendon autograft more accurately than preoperative magnetic resonance imaging for anterior cruciate ligament reconstruction.

Knee Surg Sports Traumatol Arthrosc 2021 Jan 18. Epub 2021 Jan 18.

Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, PA, USA.

Purpose: Sizing of potential autografts is essential to match the native anterior cruciate ligament (ACL) dimensions when performing ACL reconstruction (ACLR). We aimed to investigate the accuracy and reliability of the thickness and cross-sectional area (CSA) assessments for the prediction of the intraoperative diameter of the QT autograft using preoperative ultrasound and MRI.

Methods: Thirty patients (mean age ± standard deviation, 19.9 ± 5.0 years), who underwent ACLR using QT autograft, were included. The maximum thickness of the QT was assessed at 15 and 30 mm proximal using ultrasound with a long axis image, and at 15 mm proximal to the superior pole of the patella using MRI with a sagittal image. The CSA was assessed at the central 10 mm of the medial-lateral QT width at 30 mm proximal using ultrasound with a short axis image, and at 15 mm proximal to the superior pole of the patella using MRI with an axial image. Intraoperatively, QT autograft was harvested with a 10 mm width and the diameter was measured using a graft sizing device.

Results: Intra- and inter-observer reliabilities of all measurements using ultrasound and MRI were good (Intra-class correlation coefficient, 0.720-0.941). Correlation coefficient with the intraoperative diameter of the QT autograft was higher in ultrasound (R = 0.738-0.791, P < 0.001) than MRI (R = 0.449-0.543, P = 0.002-0.013).

Conclusions: Preoperative ultrasound predicted the intraoperative diameter of the QT autograft more accurately than MRI. Ultrasound may be used clinically to assure a sufficiently large QT autograft diameter to match the diameter of the patient's native ACL.

Level Of Evidence: Level III.
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http://dx.doi.org/10.1007/s00167-020-06408-4DOI Listing
January 2021

Characteristics of Femoral Torsion Angles and Relationships Between Femoral Torsion Angles and Hip Rotational Motion Variables in Adult Baseball Players.

Orthop J Sports Med 2020 Dec 18;8(12):2325967120966319. Epub 2020 Dec 18.

Department of Orthopaedic Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.

Background: Abnormalities in hip rotational motion (HRM) are risk factors for throwing injuries. To evaluate hip rotational motion, it is necessary to assess the torsion angle. However, no studies have investigated the femoral torsion angle (FTA) in baseball players.

Purpose: To investigate differences in hip FTA of adult baseball players through use of ultrasonography to evaluate the relationship between the FTA and HRM.

Study Design: Cross-sectional study; Level of evidence, 3.

Methods: A total of 149 elite male baseball players (mean age, 20.0 ± 1.8 years; 64 pitchers, 85 position players) were enrolled in this study. Ultrasonographic assessment of FTA was performed based on the tilting angles of the anterior surface of the femoral neck and condylar axis. The hip internal rotation (HIR) and hip external rotation (HER) were assessed at 0° and 90° of flexion. Data related to FTA and HRM were compared between hips and between pitchers and position players.

Results: No significant difference was observed in the FTAs between hips for any player (lead hip, 20.5° ± 9.2°; trail hip, 19.6° ± 9.8°; = .276). No significant difference was observed in FTAs of both hips between pitchers and position players (lead hip, 20.1° ± 9.4° and 20.9° ± 9.0°, respectively, = .957; trail hip, 19.5° ± 9.8° and 19.7° ± 9.8°, = .999). In terms of HER in 90° of hip flexion in both hips, significant differences were observed in HRM variables between pitchers and position players (lead hip, 33.0° ± 8.9° and 37.5° ± 9.8°, respectively, = .024; trail hip, 35.6° ± 9.6° and 40.4° ± 10.2°, = .035). Linear regression analysis revealed a significant agreement between the FTA and HIR at both 0° ( = 0.298, < .001) and 90° of hip flexion ( = 0.279, < .001).

Conclusion: Our findings indicated that FTAs were not different between the hips of elite baseball players. Abnormalities of the HRM are not caused by differences in the FTA but rather involve soft tissue tightness around the hip area or other bony morphologic factors in the hip joint.
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http://dx.doi.org/10.1177/2325967120966319DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7750773PMC
December 2020

Novel TLR2xTLR4 Bispecific Antibody Inhibits Bacterial Sepsis.

Monoclon Antib Immunodiagn Immunother 2021 Feb 21;40(1):6-10. Epub 2020 Dec 21.

Drug Discovery Research, Astellas Pharma, Inc., Tsukuba, Ibaraki, Japan.

Toll-like receptors (TLRs) sense microbial infection through recognition of pathogen-associated molecular patterns. For example, TLR4 responds to the lipopolysaccharide of gram-negative bacteria, whereas TLR2 recognizes a broad range of microbial ligands. Both receptors are, therefore, compelling targets for treating sepsis. Here, we developed a TLR2xTLR4 tetravalent bispecific antibody designated ICU-1, which inhibits both receptors. The inhibitory activity of ICU-1 was comparable to that of the parental antibodies in neutralization assays using a human monocyte cell line. Moreover, ICU-1 completely blocked stimulation of human peripheral blood mononuclear cells by clinically relevant bacterial species. These findings provide convincing evidence that ICU-1 offers a novel approach for treating bacterial sepsis.
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http://dx.doi.org/10.1089/mab.2020.0025DOI Listing
February 2021

Comparison of pleiotropic effects of statins vs fibrates on laboratory parameters in patients with dyslipidemia: A retrospective cohort study.

Medicine (Baltimore) 2020 Dec;99(50):e23427

Division of Pharmacology, Department of Biomedical Sciences, Nihon University School of Medicine.

Differences in the mechanism of action and potential pleiotropic effects between statins and fibrates would potentially drive a different effect on various laboratory parameters, but this remains controversial because of a paucity of reports comparing them. Therefore, the aim of this study was to compare the effects of statins and fibrates on laboratory parameters in Japanese patients in routine clinical practice.This retrospective cohort study included patients with dyslipidemia who had been newly treated with statin or fibrate monotherapy between January 2005 and December 2017. Patients were randomly matched into two sets of pairs by sex, age, and baseline triglyceride (TG) or low-density lipoprotein (LDL) cholesterol level. The 830 patients in TG-matched pairs (415 fibrate users and 415 matched statin users) and 1172 patients in LDL cholesterol-matched pairs (586 fibrate users and 586 matched statin users) were included in this study. Generalized estimating equations were used to estimate the effects of the drugs on serum creatinine level, estimated glomerular filtration rate (eGFR), urea nitrogen, hemoglobin A1c, aspartate aminotransferase, and alanine aminotransferase (ALT), in addition to LDL cholesterol and TG levels, and red blood cell (RBC) and platelet (PLT) counts, up to 12 months after the start of study drug administration.In TG-matched pairs, the increases in creatinine and urea nitrogen levels (P = .010 and P < .001, respectively) and the decreases in eGFR, ALT level and RBC count (P < .001, P = .003, and P = .014, respectively) were greater in fibrate users than in statin users. The decrease in PLT count was greater in statin users than in fibrate users (P < .001). The mean changes in aspartate aminotransferase and hemoglobin A1c levels were not significantly different between statin users and fibrate users. In LDL cholesterol-matched pairs, the differences in changes of all laboratory parameter levels between statin users and fibrate users were similar to those in TG-matched pairs.We demonstrate here that fibrates have a greater effect of increasing creatinine and urea nitrogen levels and of reducing eGFR, ALT level, and RBC count than statins, and that the lowering effect on PLT count is greater with statins than with fibrates.
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http://dx.doi.org/10.1097/MD.0000000000023427DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738156PMC
December 2020

Generation and Characterization of a Novel Anti-Rat TLR4/MD2 Antibody with Potent Neutralizing Activity .

Monoclon Antib Immunodiagn Immunother 2020 Dec 7;39(6):217-221. Epub 2020 Dec 7.

Drug Discovery Research, Astellas Pharma, Inc., Tsukuba, Ibaraki, Japan.

Toll-like receptor 4 (TLR4) plays a critical role in the innate immune system and is involved in the pathogenesis of multiple diseases. Here, we report the antagonistic and ratized antibody, 52-1H4 e2 (e2), which completely inhibited lipopolysaccharide-induced interleukin-6 secretion . The average serum drug concentration was above 10 μg/mL for 28 days in rats injected with e2. The novel anti-rat TLR4/myeloid differentiation factor 2 antibody, e2, may be a useful tool for investigating the role of TLR4 in rat disease models.
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http://dx.doi.org/10.1089/mab.2020.0020DOI Listing
December 2020

Anti-cancer immunotherapy using cancer-derived multiple epitope-peptides cocktail vaccination clinical studies in patients with refractory/persistent disease of uterine cervical cancer and ovarian cancer [phase 2].

Oncoimmunology 2020 11 11;9(1):1838189. Epub 2020 Nov 11.

Department of Cancer, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.

We had conducted phase 1/2 studies of cancer vaccination therapy using neo-tumor antigens in patients with refractory/persistent cervical cancer (CC) and ovarian cancer (OC) to assess the feasibility and efficacy. Enrollees must be refractory/persistent disease for usual treatments with Human Leukocyte Antigen-A*0201 or A*2402. The targets were epitope peptides obtained from driver genes in surviving pathways as follows: for CC A*0201, peptides from Up Regulating Lung Cancer 10 gene (URLC10) and Hypoxia-inducible gene 2 (HIG-2) and for OC A*0201, HIG2, VEGFR (vascular epithelial growth factor receptor) 1 and 2 were used. For CC A*2402, Forkhead Box M1 (FOXM1), Maternal Embryonic Leucine zipper Kinase (MELK), and Holliday Junction Recognition Protein (HJURP) were used. For OC A*2402, cocktails of peptides from FOXM1, MELK, HJURP, VEGFR1, and VEGFR2 were used. Subcutaneous administration was performed with adjuvant weekly. The toxicity profiles and tumor-response were analyzed in eight-week interval. Sixty-six patients were accrued, and 64 were evaluable for adverse events (AEs), and 35 for response. AEs of G2/3 dermatologic reaction (DR) of injection site had been identified in 15.6% and no other severe AEs were detected. Response rate in OC and CC were 22.9% and 20%, respectively. Median overall survival showed longer in performance status (PS) 0 (versus PS1/2), in CRP negative (versus positive) and in DR positive (versus negative) such as 8.7 m versus 1.2 m ( < .001), 8.8 m versus 3.0 m ( < .05) and 10.2 m versus 1.2 m ( < .001), respectively. In conclusion, our vaccination therapy was feasible and effective in this cohort of patients.
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http://dx.doi.org/10.1080/2162402X.2020.1838189DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7671072PMC
November 2020

Coherent Vibration and Femtosecond Dynamics of the Platinum Complex Oligomers upon Intermolecular Bond Formation in the Excited State.

Angew Chem Int Ed Engl 2020 Dec 16;59(51):23154-23161. Epub 2020 Nov 16.

Molecular Spectroscopy Laboratory, RIKEN, 2-1 Hirosawa, Wako, 351-0198, Japan.

Femtosecond time-resolved absorption and picosecond time-resolved emission measurements were carried out for highly concentrated aqueous solutions of K [Pt(CN) ] to investigate excited-state dynamics of the [Pt(CN) ] oligomers formed with metallophilic interactions. Time-resolved absorption spectra exhibit complicated dynamics that are represented with five time constants. Among them, the 90-ps and 400-ps dynamics were assigned to the S → T intersystem crossing of the trimer and tetramer coexisting in the solution by comparison with the fluorescence decays. Clear oscillations of transient absorption were observed in the first few picoseconds, and the frequency-detected-wavelength 2D analysis revealed that the 135-cm and 65-cm oscillations arise from the Pt-Pt stretch motions of the S trimer and S tetramer, respectively. The obtained time-resolved spectroscopic data provide a clear view of the excited-state dynamics of the [Pt(CN) ] oligomers in the femto-/picosecond time region.
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http://dx.doi.org/10.1002/anie.202011813DOI Listing
December 2020

Phase II trial of combination treatment with S-1/cetuximab in patients with platinum-ineligible recurrent and/or metastatic squamous cell carcinoma of the head and neck.

Int J Clin Oncol 2021 Jan 29;26(1):51-58. Epub 2020 Sep 29.

Department of Medical Oncology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, North 15 West 7, Kita-ku, Sapporo, 060-8638, Japan.

Background: The standard of care for first-line treatment of recurrent and/or metastatic squamous cell carcinoma of the head and neck (R/M SCCHN) in patients who cannot tolerate platinum-based regimens has not been clarified. We aimed to develop a new treatment regimen for patients with R/M SCCHN who are ineligible for platinum-based therapy, by evaluating the effects and safety of tegafur/gimeracil/oteracil (S-1) and cetuximab.

Methods: Platinum-ineligibility was defined as: elderly (aged ≥ 75 years), poor PS, comorbidity, platinum resistance and refusal to undergo platinum-based therapy. Patients received S-1 (80 mg/m/day for 14 days followed by a seven-day break) and cetuximab (initial dose, 400 mg/m, followed by 250 mg/m weekly) until disease progression or unacceptable toxicity. The primary endpoint was overall response rate (ORR).

Results: Between September 2014 and September 2018, we enrolled 23 patients. Among the 21 patients who were evaluable, 20 were male [median age, 69 years (range 49-82)]. The ORR was 9 (43%) of 21 patients [95% confidence interval (CI) 22-66]. One and eight patients achieved complete response (CR) and partial response (PR), respectively. The median overall survival (OS) was 13.7 months (95% CI 9.0-18.3) and progression-free survival (PFS) was 5.7 months (95% CI 3.1-8.2). Grade 3/4 adverse events included acneiform rash and skin reactions (33%), hypomagnesemia (19%), hand-foot syndrome (14%), fatigue (14%), mucositis (10%), and anorexia (10%).

Conclusions: Combination treatment with S-1 and cetuximab was effective and tolerated well by patients with platinum-ineligible R/M SCCHN. Registered clinical trial number: UMIN000015123.
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http://dx.doi.org/10.1007/s10147-020-01788-6DOI Listing
January 2021

Time-Domain Observation of Surface-Enhanced Coherent Raman Scattering with 10-10 Enhancement.

J Phys Chem Lett 2020 Aug 23;11(15):6305-6311. Epub 2020 Jul 23.

Molecular Spectroscopy Laboratory, RIKEN, 2-1 Hirosawa, Wako 351-0198, Japan.

Combining surface-enhanced Raman scattering (SERS) with the coherent nonlinear Raman technique is a promising route for achieving higher sensitivity and time-resolved SERS measurements, yet such attempts have just been started. Here, we report time-domain Raman measurements of -1,2-bis(4-pyridyl)ethylene (BPE) adsorbed on gold nanoparticle assemblies (GNAs), which were carried out with impulsive stimulated Raman spectroscopy using sub-8 fs pulses. We observe coherent nuclear wavepacket motion of BPE on GNAs with drastic enhancement through the surface plasmon resonance, which provides information on the Raman-active vibrations in the time domain. Through Fourier transform of the measured time-domain Raman data, we obtained SERS spectra of BPE on GNAs with enhancement factors as high as 10-10. The present study not only demonstrates applicability of time-domain nonlinear Raman techniques in SERS, i.e., surface-enhanced impulsive stimulated Raman spectroscopy (SE-ISRS), but also provides a technical basis for femtosecond time-resolved SE-ISRS experiments to track ultrafast dynamics of the adsorbates.
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http://dx.doi.org/10.1021/acs.jpclett.0c01411DOI Listing
August 2020

Femtosecond Polarization Switching in the Crystal of a [CrCo] Dinuclear Complex.

Angew Chem Int Ed Engl 2020 Sep 29;59(37):15865-15869. Epub 2020 Jun 29.

Molecular Spectroscopy Laboratory, RIKEN, and, Ultrafast Spectroscopy Research Team, RIKEN Center for Advanced Photonics (RAP), 2-1 Hirosawa, Wako, 351-0198, Japan.

Capability to control macroscopic molecular properties with external stimuli offers the possibility to exploit molecules as switching devices of various types. However, application of such molecular-level switching has often been limited by its speed and thus efficiency. Herein, we demonstrate ultrafast, photoinduced polarization switching in the crystal of a [CrCo] dinuclear complex by ultrafast pump-probe spectroscopy in the visible and mid-infrared regions. The photoinduced polarization switching was found to have a time constant of 280 fs, which makes the [CrCo] complex crystal the fastest polarization-switching material realized using the metastable state. Moreover, the pump-probe data in the visible region reveal the pronounced appearance of coherent nuclear wavepacket motion with a frequency as low as 22 cm , which we attribute to a lattice vibrational mode. The pronounced non-Condon effect for its resonance Raman enhancement implies that this mode couples the relevant electronic states, thereby facilitating the ultrafast polarization switching.
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http://dx.doi.org/10.1002/anie.202004583DOI Listing
September 2020

Phase 1/2 study of concurrent chemoradiotherapy with weekly irinotecan hydrochloride for advanced/recurrence uterine cancer: A multi-institutional study of Kansai Clinical Oncology Group.

Chin J Cancer Res 2020 Apr;32(2):218-227

Department of Obstetrics and Gynecology, National Hospital Organization Kobe Medical Center, Kobe 6540155, Japan.

Objective: Concurrent chemoradiotherapy using cisplatin was thought to be standard treatment for squamous cell carcinoma of cervix, but it had not been effective for adenocarcinoma. Concurrent chemoradiotherapy using irinotecan hydrochloride (CPT-11) had been effective for colorectal cancer, thus, we chose CPT-11 as a candidate for gynecologic adenocarcinoma. To evaluate the maximum tolerated dose (MTD) of weekly CPT-11 with external pelvic radiotherapy, a phase 1/2 study was conducted according to modified Fibonacci method.

Methods: Eligible patients were advanced uterine cancer with measurable diseases [performance score (PS): 0-2]. Study period was from August 1st, 2002 to December 31st, 2008. The starting dose level (DL) of CPT-11 was 30 mg/m (DL1) given weekly for 4 weeks. Subsequently, dose escalation was scheduled in 10 mg/m increments to 60 mg/m (DL4). The fixed radiotherapy consisted of whole pelvic 1.8 Gy/d, once a day in weekday for five weeks and it amounted to 45 Gy (25 fractions) in total.

Results: Seventeen patients were enrolled. As for toxicities, one (1/17: 5.9%) grade (G) 4 neutropenia lasting 7 days had been seen in DL4. G2 diarrhea was identified in 35.3% (6/17) of the patients, and 11.8% (2/17) G3 diarrhea was observed in DL3 and DL4. Thus, the MTD of CPT-11 was defined as dose of 60 mg/m. The recommended dose was decided as 50 mg/m. The response rate was 88.2% [9 complete response (CR), 3 partial response (PR), 3 stable disease (SD), 2 not evaluable (NE)]. Disease control rate at 1 month after treatment completion was 100% but distant metastases were found in 24% (4/17) in longer outcome.

Conclusions: MTD was 60 mg/m and recommended dose was set as 50 mg/m. This concurrent chemoradiation using weekly CPT-11 was feasible at 50 mg/m, and it might be effective even in adenocarcinoma of the uterus.
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http://dx.doi.org/10.21147/j.issn.1000-9604.2020.02.09DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7219098PMC
April 2020

In situ cross-sectional area of the quadriceps tendon using preoperative magnetic resonance imaging significantly correlates with the intraoperative diameter of the quadriceps tendon autograft.

Knee Surg Sports Traumatol Arthrosc 2021 Mar 24;29(3):742-749. Epub 2020 Apr 24.

Department of Orthopaedic Surgery, University of Pittsburgh, Kaufman Medical Building, Suite 1011, 3941 Fifth Avenue, Pittsburgh, PA, 15203, USA.

Purpose: Preoperative assessment to determine the sizes of potential autografts is necessary for individualized anterior cruciate ligament reconstruction (ACLR). However, no study has investigated the prediction of the intraoperative diameter of the quadriceps tendon (QT) autograft based upon preoperative imaging. This study investigated the correlation between the intraoperative diameter of a QT autograft and in situ thickness or cross-sectional area (CSA) measured using preoperative MRI.

Methods: Thirty-one knees of 31 patients (mean age 20.9 ± 5.0 years) who underwent individualized anatomic ACLR using all soft tissue QT autograft were included retrospectively. At 15 mm proximal to the superior pole of the patella, the maximum QT thickness was assessed in the sagittal plane and the CSA was assessed at the central 10 mm of the QT in the axial plane. The angle between the axial plane and a line perpendicular to the QT longitudinal axis was used to calculate an adjusted CSA using a cosine function. Intraoperatively, each QT autograft was harvested with 10 mm width and the diameter was measured using a graft sizing device.

Results: Intra- and inter-observer reliabilities of all measurements using preoperative MRI were excellent (intra-class correlation coefficient, 0.833-0.970). Significant correlations were observed between the thickness, CSA, or adjusted CSA, and the intraoperative diameter (R = 0.434, 0.607, and 0.540, respectively; P < 0.05).

Conclusions: The CSA correlated most strongly with the QT autograft diameter. For individualized anatomic ACLR, measuring in situ CSA can be useful for preoperative planning of appropriate graft choices prior to surgery.

Level Of Evidence: III.
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http://dx.doi.org/10.1007/s00167-020-05993-8DOI Listing
March 2021

Prevalence, clinical course, and predictive factors of immune checkpoint inhibitor monotherapy-associated hepatitis in Japan.

J Gastroenterol Hepatol 2020 Oct 31;35(10):1782-1788. Epub 2020 Mar 31.

Department of Gastroenterology and Hepatology, Graduate School of Medicine, Hokkaido University, Sapporo, Japan.

Background And Aim: Immune checkpoint inhibitors (ICI) have revolutionized anti-malignancy therapy and thus have been increasingly used. Although ICI may cause immune-related adverse events (irAE) in various organs, including the liver, the prevalence and predictive factors of irAE have not been clarified.

Methods: In this retrospective study, consecutive patients who had malignancies and were treated with ICI without other chemotherapeutic agents at Hokkaido University Hospital between 2014 and 2019 were screened. Patients were excluded if they were < 20 years old and had insufficient clinical data.

Results: Of the 233 patients screened, 202 patients met the inclusion criteria and were included in the analysis. The patients were aged 25-92 years, and 60.9% were male. The patients received nivolumab (n = 137), pembrolizumab (n = 45), ipilimumab (n = 17), atezolizumab (n = 2), and avelumab (n = 1). The prevalence of any grade and grade ≥ 3 irAE hepatitis was 8.4% (17/202) and 4.0% (8/202), respectively. irAE hepatitis occurred at a median duration of 42 days in any grade and 36 days in grade ≥ 3 after ICI initiation. The clinical course of grade ≥ 3 irAE hepatitis was generally favorable; however, 50% required corticosteroid treatment and two patients required additional mycophenolate mofetil. Female sex and history of ICI treatment were significantly associated with the incidence of grade ≥ 3 irAE hepatitis.

Conclusions: Grade ≥ 3 irAE hepatitis was observed in 4.0% of the patients who were treated with ICI. Female sex and history of ICI treatment were significantly associated with the incidence of grade ≥ 3 irAE hepatitis.
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http://dx.doi.org/10.1111/jgh.15041DOI Listing
October 2020

A phase 2 study of intraperitoneal carboplatin plus intravenous dose-dense paclitaxel in front-line treatment of suboptimal residual ovarian cancer.

Br J Cancer 2020 03 31;122(6):766-770. Epub 2020 Jan 31.

Department of Gynecologic Oncology, Saitama Medical University International Medical Center, Hidaka, Saitama, 350-1298, Japan.

Background: We evaluated the efficacy of intraperitoneal (IP) carboplatin in combination with dose-dense paclitaxel (ddTCip) for suboptimal residual ovarian cancer.

Methods: This was a phase 2 study to evaluate ddTCip. Patients with stage II-IV ovarian carcinoma, who underwent primary cytoreductive surgery and had radiologically evaluable disease after surgery, were eligible to participate in this study. IP carboplatin (AUC = 6) was administered on day 1, and intravenous paclitaxel (80 mg/m) was administered on days 1, 8 and 15. The primary endpoint was response rate. Secondary endpoints included progression-free survival (PFS), overall survival (OS) and safety. Interval- debulking surgery followed by the same regimen was allowed when indicated.

Results: A total of 117 patients were considered eligible for this study prior to surgery and temporarily registered. Of the 117 patients, 76 patients met the inclusion criteria and were enrolled in this study. Fifty-nine (83.1%) patients had objective clinical responses. Median PFS and OS were 18.3 and 55.5 months, respectively. Sixty-four (84.2%) patients had grade 3/4 neutropenia, 43 (56.5%) patients had anaemia and 17 (22.4%) patients had thrombocytopenia. Port-related adverse events occurred in nine (11.8%) patients.

Conclusions: Front-line chemotherapy with ddTCip therapy appears safe and effective, even for patients with suboptimal residual ovarian cancer.

Trial Registration: UMIN Clinical Trials Registry (ID: UMIN000001713) on February 16th, 2009.
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http://dx.doi.org/10.1038/s41416-020-0734-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7078205PMC
March 2020

Metachronous liver metastases after long-term follow-up of endoscopic resection for rectal neuroendocrine neoplasms: a report of three cases.

Surg Case Rep 2020 Jan 15;6(1):22. Epub 2020 Jan 15.

Department of Gastroenterological Surgery II, Division of Surgery, Faculty of Medicine, Hokkaido University, West-7, North-15, Kita-ku, Sapporo, 060-8638, Japan.

Background: Rectal neuroendocrine neoplasms (NENs) are rare, but their incidence has increased in recent years. The metastasis rate is low in cases of a tumor diameter < 1 cm or depth of invasion lower than the submucosa; therefore, the European Neuroendocrine Tumor Society (ENETS) and the North American Neuroendocrine Tumor Society (NANETS) consensus guidelines recommend endoscopic resection. Since little has been reported on the long-term prognosis of endoscopic resection for rectal NEN, consensus is lacking regarding the follow-up period after endoscopic resection.

Case Presentation: Here, we report three cases of metachronous liver metastasis after long-term follow-up of endoscopic mucosal resection (EMR) for rectal NEN. The pathological findings indicated a depth lower than the submucosa and complete radical resection in all cases and lymphovascular invasion in only one case. All three cases showed metachronous multiple liver metastases after 9-13 years of follow-up for EMR, despite achieving complete resection and without muscular invasion.

Conclusions: Metachronous liver metastases may occur after long interval following endoscopic resection; thus, long-term follow-up is necessary after endoscopic resection for rectal NEN.
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http://dx.doi.org/10.1186/s40792-020-0792-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6962411PMC
January 2020

Tracking Photoinduced Au-Au Bond Formation through Transient Terahertz Vibrations Observed by Femtosecond Time-Domain Raman Spectroscopy.

J Am Chem Soc 2019 Dec 27;141(49):19296-19303. Epub 2019 Nov 27.

Molecular Spectroscopy Laboratory , RIKEN , 2-1 Hirosawa , Wako 351-0198 , Japan.

Real-time observation of chemical bond formation and subsequent nuclear rearrangements is an ultimate goal of chemical science. Yet, such attempts have been hampered by the technical difficulty of triggering bond formation at well-defined, desired timing. The trimer of dicyanoaurate complex ([Au(CN)]) is an ideal system for achieving this aim because the tight covalent Au-Au bonds are formed upon photoexcitation. Despite the apparent simplicity of the system, however, recent time-resolved studies failed to construct a consistent picture of its ultrafast dynamics. Here, we report femtosecond time-domain Raman tracking of ultrafast structural dynamics of the [Au(CN)] trimer upon photoinduced Au-Au bond formation. The obtained Raman data reveal that the Au-Au breathing vibration at ∼90 cm exhibits a gradual frequency upshift in a few picoseconds, demonstrating a continuous bent-to-linear structural change on the triplet-state potential energy surface upon the Au-Au bond formation. The comprehensive ultrafast spectroscopic study settles the controversy on this prototypical molecular assembly.
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http://dx.doi.org/10.1021/jacs.9b06950DOI Listing
December 2019

Protein Dynamics Preceding Photoisomerization of the Retinal Chromophore in Bacteriorhodopsin Revealed by Deep-UV Femtosecond Stimulated Raman Spectroscopy.

J Phys Chem Lett 2019 Sep 30;10(18):5422-5427. Epub 2019 Aug 30.

Molecular Spectroscopy Laboratory , RIKEN , 2-1 Hirosawa , Wako 351-0198 , Japan.

Bacteriorhodopsin is a prototypical photoreceptor protein that functions as a light-driven proton pump. The retinal chromophore of bacteriorhodopsin undergoes C═C trans-to-cis isomerization upon photoexcitation, and it has been believed to be the first event that triggers the cascaded structural changes in bacteriorhodopsin. We investigated the protein dynamics of bacteriorhodopsin using deep-ultraviolet resonance femtosecond stimulated Raman spectroscopy. It was found that the stimulated Raman signals of tryptophan and tyrosine residues exhibit significant changes within 0.2 ps after photoexcitation while they do not noticeably change during the isomerization process. This result implies that the protein environment changes first, and its change is small during isomerization. The obtained femtosecond stimulated Raman data indicate that ultrafast change is induced in the protein part by the sudden creation of the large dipole of the excited-state chromophore, providing an environment that realizes efficient and selective isomerization.
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http://dx.doi.org/10.1021/acs.jpclett.9b02283DOI Listing
September 2019

Serum squamous cell carcinoma antigen (SCCA)-2 correlates with clinical severity of pediatric atopic dermatitis in Ishigaki cohort.

J Dermatol Sci 2019 Aug 24;95(2):70-75. Epub 2019 Jul 24.

Department of Dermatology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.

Background: We sometimes encounter difficulties in assessing the severity of pediatric atopic dermatitis (AD) using currently available biomarkers such as thymus and activation-regulated chemokine (TARC) due to the higher baseline values in non-AD children. Recent case control studies have indicated the usefulness of squamous cell carcinoma antigens (SCCAs) in pediatric and adult AD. Notably, SCCAs are induced by IL-4 and IL-13, vital Th2 cytokines that play important roles in AD etiology.

Objectives: Relatively low prevalence and mild disease severity of pediatric AD are observed in our Ishigaki cohort presumably due to the moisturising subtropical climate, which could conversely mean possible higher allergic potential of non-AD subjects towards AD. Thus, the purpose of this study was to further investigate the feasibility of using SCCAs together with TARC and periostin as biomarkers for pediatric AD even in the Ishigaki cohort.

Methods: We enrolled 1459 nursery school children and identified 96 as having AD through 2009-2011. As statistical analyses, we performed Student's t-test, correlation analysis, and receiver and operating characteristic (ROC) analysis.

Results: Serum SCCA1, SCCA2, periostin and TARC levels were all significantly increased in AD compared with those in non-AD, but only serum SCCA2 showed a significant increase in AD when assessed in each age group or in subgroup analysis. Among the biomarkers tested, serum SCCA2 also showed the best correlations with clinical AD severity and TARC and showed the best diagnosability for AD in ROC analysis.

Conclusions: SCCA2 is a potent biomarker for pediatric AD in the Ishigaki cohort.
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http://dx.doi.org/10.1016/j.jdermsci.2019.07.005DOI Listing
August 2019

Tumor characteristics and outcome of uterine carcinosarcoma in women aged ≥80 years.

Surg Oncol 2019 Jun 7;29:25-32. Epub 2019 Feb 7.

Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, Magee-Womens Hospital, University of Pittsburgh, PA, USA.

Objective: To examine clinico-pathological characteristics and outcomes of uterine carcinosarcoma (UCS) in women aged ≥80 years.

Methods: This is a secondary analysis of a previous multicenter retrospective study examining 906 women with stage I-IV UCS who underwent primary hysterectomy. Patient demographics, treatment types, tumor characteristics, and survival were examined across aged ≥80 (n = 82 [9.1%]), aged 60-79, (n = 526 [58.1%]), and aged <60 (n = 298 [32.9%]).

Results: Women in the aged ≥80 group were more likely to be Caucasian, undergo simple hysterectomy without lymphadenectomy, and receive no postoperative therapy (all, P < 0.05). Tumors in the aged ≥80 group were more likely to have high-grade carcinoma, heterologous sarcoma, and sarcoma dominance but less likely to have lympho-vascular space invasion (all, P < 0.05). Lymphadenectomy did not improve survival in the aged ≥80 group (P > 0.05), whereas lymphadenectomy was protective for survival in the younger groups (both, P < 0.05). Postoperative chemotherapy was associated with improved progression-free survival (PFS) in the aged ≥80 group (hazard ratio [HR] 0.44, 95% confidence interval [CI] 0.22-0.89, P = 0.021). With chemotherapy treatment, women in the aged ≥80 group had PFS similar to those in the aged 60-79 group (HR 0.97, 95%CI 0.51-1.83, P = 0.92). In contrast, without chemotherapy treatment, women in the aged ≥80 group had significantly decreased PFS compared to the aged 60-79 group (HR 1.62, 95%CI 1.09-2.40, P = 0.016). Similar associations were observed for postoperative radiotherapy.

Conclusion: Nearly 10% of women with UCS are aged ≥80 that are characterized by aggressive tumor factors. Postoperative therapy but not extensive surgery may improve survival in this age group.
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http://dx.doi.org/10.1016/j.suronc.2019.02.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7523232PMC
June 2019

Fifth-order time-domain Raman spectroscopy of photoactive yellow protein for visualizing vibrational coupling in its excited state.

Sci Adv 2019 06 7;5(6):eaau4490. Epub 2019 Jun 7.

Molecular Spectroscopy Laboratory, RIKEN, 2-1 Hirosawa, Wako 351-0198, Japan.

We report fifth-order time-domain Raman spectroscopy of photoactive yellow protein (PYP), with the aim to visualize vibrational coupling in its excited state. After the ultrashort actinic pump pulse prepared the vibrational coherence and population in the excited state, the evolving vibrational structure was tracked by time-resolved impulsive stimulated Raman spectroscopy using sub-7-fs pulses. The obtained fifth-order time-domain Raman data were translated to a two-dimensional (2D) frequency-frequency correlation map, which visualizes the correlation between low- and high-frequency vibrational modes of the excited state. The 2D map of PYP reveals a cross peak, indicating the coupling between the phenolic C─O stretch mode of the chromophore and the low-frequency modes (~160 cm), assignable to the intermolecular motions involving the surrounding hydrogen-bonded amino acids. The unveiled coupling suggests the importance of the low-frequency vibrational motion in the primary photoreaction of PYP, highlighting the unique capability of this spectroscopic approach for studying ultrafast reaction dynamics.
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http://dx.doi.org/10.1126/sciadv.aau4490DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6555629PMC
June 2019

Ultrasonographic Assessment of Femoral Torsion Angle Based on Tilting Angles of Femoral Neck and Condylar Axis.

Ultrasound Med Biol 2019 08 4;45(8):1970-1976. Epub 2019 May 4.

Department of Orthopaedic Surgery, Nagoya City University Graduate School of Medical Science, Nagoya, Japan. Electronic address:

Femoral anteversion has been assessed with ultrasound using femoral neck tilting angle (FN-TA); however, femoral torsion angle (FTA), which is defined using FN-TA and condylar axis tilting angle, has not been assessed with ultrasound. This study aimed to establish the ultrasonographic assessment of FTA (US-FTA) by comparing data obtained through US-FTA and computed tomography (CT). Twenty-one patients (age range, 38-82 y) with 21 intact hips were included. In the US-FTA, the femoral head and anterior tubercle of the greater trochanter were used as bony landmarks. The intra-rater and inter-rater reliabilities and standard error of measurement (SEM) of US-FTA were 0.994 (SEM 0.93) and 0.994 (SEM 0.94), respectively. A strong agreement was found between FTA variables obtained with ultrasound and CT (R = 0.939, p < 0.001). Ultrasound is useful and can be a valid alternative to CT for the evaluation of the femoral torsion angle without radiation exposure.
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http://dx.doi.org/10.1016/j.ultrasmedbio.2019.03.022DOI Listing
August 2019
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