Publications by authors named "Sari M-R Tuusa"

2 Publications

  • Page 1 of 1

Reconstruction of critical size calvarial bone defects in rabbits with glass-fiber-reinforced composite with bioactive glass granule coating.

J Biomed Mater Res B Appl Biomater 2008 Feb;84(2):510-9

Department of Prosthetic Dentistry and Biomaterials Science, Institute of Dentistry, University of Turku, Turku, Finland.

Unlabelled: The aim of this study was to evaluate glass-fiber-reinforced composite as a bone reconstruction material in the critical size defects in rabbit calvarial bones. The bone defect healing process and inflammatory reactions were evaluated histologically at 4 and 12 weeks postoperatively. Possible neuropathological effects on brain tissue were evaluated. The release of residual monomers from the fiber-reinforced composite (FRC) was analyzed by high performance liquid chromatograph (HPLC).

Results: At 4 weeks postoperatively, fibrous connective tissue ingrowth to implant structures was seen. Healing had started as new bone formation from defect margins, as well as woven bone islets in the middle of the defect. Woven bone was also seen inside the implant. Inflammation reaction was slight. At 12 weeks, part of the new bone had matured to lamellar-type, and inflammation reaction was slight to moderate. Control defects had healed by fibrous connective tissue. Histological examinations of the brain revealed no obvious damage to brain morphology. In HPLC analysis, the release of residual 1,4-butanedioldimethacrylate and methylmethacrylate from polymerized FRC was low.

Conclusions: This FRC-implant was shown to promote the healing process of critical size calvarial bone defect in rabbits. After some modifications to the material properties, this type of implant has the potential to become an alternative for the reconstruction of bone defects in the head and neck area in the future.
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February 2008

Residual monomers released from glass-fibre-reinforced composite photopolymerised in contact with bone and blood.

J Mater Sci Mater Med 2005 Jan;16(1):15-20

Department of Prosthetic Dentistry & Biomaterials Research, Institute of Dentistry, University of Turku, Turku, Finland.

Purpose: The aim of this study was to determine the quantity of residual monomers of glass fibre-reinforced composite released into water from the composite that had been photopolymerized in contact with bone and blood.

Materials And Methods: E-glass fibre reinforced composite (FRC) made of E-glass fibre veil and the bis-GMA-TEGDMA-PMMA resin system was used in the study. In the first group, pieces of non-polymerised FRC were photopolymerised (40 s) in air which influenced the oxygen inhibited resin layer (positive control). In the second group, the FRC was polymerized between two glass plates allowing both surfaces to be well polymerized (negative control). In the test groups, the FRC was polymerized in contact with bone or in contact with blood. FRC specimens from all four groups were incubated in three milliliters of deionised water at 37 degrees C for three days. At the end of the incubation period, the residual monomers were extracted from the water with dichloromethane, and the residual monomers of TEGDMA and bis-GMA quantitatively analysed by HPLC. The degree of monomer conversion was measured by FTIR from the surface of the test specimen. Differences between the groups were analysed using one-way ANOVA (p < 0.05).

Results: The total quantity of residual monomers released from FRC polymerized in contact with bone was lower (0.55 wt%) than in the positive control group (0.97 wt%) (p = 0.021), and only slightly exceeded that of the negative control group (0.42 wt%) (p = 0.717). The total quantity of monomers released from FRC polymerized in contact with blood was at the level of the negative control group. The main residual monomer released was TEGDMA. The surfaces of the positive and negative controls showed a clear difference between the degree of monomer conversion, 34.0 and 62.8%, respectively, when analysed with FTIR (p < 0.001).

Conclusion: The surface of the bone or contact with blood did not significantly inhibit the photoinitiated free radical polymerisation of the dimethacrylate monomer system of the FRC.
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January 2005