Publications by authors named "Sarah Sucayan"

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Direct observation of dynamic mechanical regulation of DNA condensation by environmental stimuli.

Angew Chem Int Ed Engl 2014 Sep 21;53(40):10631-5. Epub 2014 Aug 21.

Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742 (USA).

Gene delivery is a promising way to treat hereditary diseases and cancer; however, there is little understanding of DNA:carrier complex mechanical properties, which may be critical for the protection and release of nucleic acids. We applied optical tweezers to directly measure single-molecule mechanical properties of DNA condensed using 19-mer poly-L-lysine (PLL) or branched histidine-lysine (HK) peptides. Force-extension profiles indicate that both carriers condense DNA actively, showing force plateaus during stretching and relaxation cycles. As the environment such as carrier concentration, pH, and the presence of zinc ions changes, DNA:HK complexes showed dynamically regulated mechanical properties at multiple force levels. The fundamental knowledge from this study can be applied to design a mechanically tailored complex which may enhance transfection efficiency by controlling the stability of the complex temporally and spatially.
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September 2014