Publications by authors named "Samar El-Kholy"

12 Publications

  • Page 1 of 1

Alveolar ridge preservation using autogenous whole-tooth versus demineralized dentin grafts: A randomized controlled clinical trial.

Clin Oral Implants Res 2021 May 1;32(5):539-548. Epub 2021 Mar 1.

Oral Medicine and Periodontology Department, Faculty of Dentistry, Cairo University, Cairo, Egypt.

Objective: The objective of this randomized controlled trial was to evaluate the radiographic changes and histologic healing following alveolar ridge preservation (ARP) using autogenous whole tooth (AWTG), test group, versus autogenous demineralized dentin graft (ADDG), control group.

Material And Methods: Twenty non-molar teeth indicated for extraction were randomized into two groups (n = 10/group). Extracted teeth were prepared into AWTG or ADDG (0.6N HCl; 30 min), inserted into extraction sockets and covered by collagen membranes. Cone-beam computed tomography (CBCT) scans at baseline and six months were compared to assess ridge-dimensional changes. At six months, bone biopsies of engrafted sites were harvested and analyzed histomorphometrically.

Results: All sites healed uneventfully. Reduction was 0.85 ± 0.38 mm and 1.02 ± 0.45 mm in ridge width, 0.61 ± 0.20 mm and 0.72 ± 0.27 mm in buccal and 0.66 ± 0.31 mm and 0.56 ± 0.24 mm in lingual ridge height for the AWTG and ADDG group, respectively (p > .05). Histologically, no inflammatory reactions were noticeable and all samples showed new bone formation. Qualitatively, graft-bone amalgamations were more pronounced in ADDG samples. Histomorphometrically, new bone, graft remnants and soft tissue occupied 37.55% ± 8.94%, 17.05% ± 5.58% and 45.4% ± 4.06% of the areas in the AWTG group and 48.4% ± 11.56%, 11.45% ± 4.13% and 40.15% ± 7.73% in the ADDG group of the examined areas, respectively (p > .05).

Conclusions: AWTG and ADDG are similarly effective in ARP. Yet, histologically ADDG seems to demonstrate better graft remodeling, integration and osteoinductive properties.
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http://dx.doi.org/10.1111/clr.13722DOI Listing
May 2021

Consequences of a short-term exposure to a sub lethal concentration of CdO nanoparticles on key life history traits in the fruit fly (Drosophila melanogaster).

J Hazard Mater 2021 05 26;410:124671. Epub 2020 Nov 26.

Zoology Department, Faculty of Science, Tanta University, 31527 Tanta, Egypt; General Zoology, Institute for Biology, Martin Luther University Halle-Wittenberg, Hoher Weg 8, 06120 Halle (Saale), Germany. Electronic address:

Nanoparticles of cadmium oxide (CdO NPs) are among the most common industrial metal oxide nanoparticles. Early adulthood (P1) fruit flies (D. melanogaster) were exposed for 7 days to a sub lethal concentration (0.03 mg CdO NPs/mL, which was 20% of the LC), spiked into food media to test whether short episodes of CdO NPs exposures early in adult life have long-lasting effects on life history traits such as fecundity well beyond exposure times. All studied life history traits, as well as climbing behavior were adversely affected by exposure to CdO NPs. A blistered wing phenotype was also observed in the non-exposed progeny (F1) of adult flies (P1) and their fecundity was significantly decreased (-50%) compared to the fecundity of non-exposed (control) F1 flies. Expressions of antioxidant enzymes encoding genes; catalase and superoxide dismutase (SOD2) were significantly up regulated in P1 flies compared to control. Expression of metallothionein encoding genes (MTn A-D) were significantly up-regulated in both parent flies (P1) and their progeny (F1) after exposure of P1 flies to CdO NPs compared to non-exposed control flies, suggesting long-term potential effects. Taken together, these findings indicate that short-term exposure to a sub-lethal CdO NP concentration is sufficient to have long-lasting, adverse effects on fruit flies.
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http://dx.doi.org/10.1016/j.jhazmat.2020.124671DOI Listing
May 2021

WITHDRAWN: Reduced fitness of the mosquito Culex pipiens (Diptera: Culicidae) after feeding on a blood meal with hepatitis C virus.

J Invertebr Pathol 2021 Jan 28:107522. Epub 2021 Jan 28.

Department of Zoology, Faculty of Science, Tanta University, Tanta 31527, Egypt.

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http://dx.doi.org/10.1016/j.jip.2020.107522DOI Listing
January 2021

Botulinum toxin type A in chronic non-dyshidrotic palmar eczema: A side-by-side comparative study.

J Dermatol 2020 Jun 14;47(6):601-608. Epub 2020 Apr 14.

Department of Dermatology, Venereology and Andrology, Faculty of Medicine, Alexandria University, Alexandria, Egypt.

New indications are being reported for intradermal botulinum toxin type A (BTX-A) owing to its anti-inflammatory and antipruritic actions. Its successful use for dyshidrotic hand eczema and lichen simplex has been reported in a few cases, while its utility in dry palmar eczema not associated with hyperhidrosis has not yet been investigated. The aim of this study was the assessment of the additive efficacy and tolerability of BTX-A in chronic dry palmar eczema. This prospective non-randomized side-by-side comparative study included 30 cases of chronic bilateral dry palmar eczema with no associated hyperhidrosis. Combined emollients and topical mid-potency steroid on one side were compared with an additive 100 units of intradermal BTX-A on the other side for efficacy and tolerability using both patient- and physician-oriented scores over a period of 6 months. Timing and extent of improvement and relapse were recorded on both sides, together with the frequency of development of side-effects. Both lines were effective and well tolerated, with significantly greater reduction of symptom and sign scores and higher overall patient satisfaction on the side receiving BTX-A, an effect which lasted for a significantly longer duration on this side (4 months) as compared with the other side (1 month). In conclusion, intradermal BTX-A at a dose of 100 units/palm is beneficial and well tolerated in chronic dry palmar eczema. Compared with topical steroid and emollients alone, its addition yielded superior efficacy that was longer lasting and more satisfactory to the patients, while exerting a steroid-sparing effect.
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http://dx.doi.org/10.1111/1346-8138.15344DOI Listing
June 2020

Hormonal modulation of cannibalistic behaviors in mosquito (Culex pipiens) larvae.

J Insect Physiol 2018 Aug - Sep;109:144-148. Epub 2018 Aug 2.

Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt.

Cannibalism has been observed occasionally in a limited number of different animal species, but the underlying mechanisms that foster this behavior are mostly unknown. Here we show that mosquito (Culex pipiens) larvae show this behavior towards conspecifics under certain conditions. Cannibalism was only observed in 4th instar larvae and only in response to starvation. Well fed animals never showed any cannibalistic behavior. Starvation induced cannibalism of Culex 4th instar was predominantly directed towards 3rd instars rather than to 1st or 2nd instar larvae. Specific mandibular structures of these larvae enable this cannibalistic behavior. We could show that treatment with the biogenic amine octopamine, which is known to be involved in the control of starvation and aggression, increased the rate of cannibalism of food-deprived 4th instar larvae significantly. Incubation with the octopamine receptor antagonist phentolamine suppressed this cannibalistic behavior. Moreover, octopamine not only increased the rate of cannibalism, it also induced a shift towards smaller prey. A role of octopamine in this starvation induced behavior was further supported by direct measurements of the total content of this important neuroactive compound. Taken together, we could show that 4th instar mosquito larvae showed cannibalistic behavior after starvation and that this behavior apparently depends on octopamine.
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http://dx.doi.org/10.1016/j.jinsphys.2018.08.001DOI Listing
September 2019

Impaired Wnt signaling in dopamine containing neurons is associated with pathogenesis in a rotenone triggered Drosophila Parkinson's disease model.

Sci Rep 2018 02 5;8(1):2372. Epub 2018 Feb 5.

Kiel University, Zoological Institute, Department Molecular Physiology, Kiel, Germany.

Parkinson's disease, which is the one of the most common neurodegenerative movement disorder, is characterized by a progressive loss of dopamine containing neurons. The mechanisms underlying disease initiation and development are not well understood and causative therapies are currently not available. To elucidate the molecular processes during early stages of Parkinson's disease, we utilized a Drosophila model. To induce Parkinson's disease-like phenotypes, we treated flies with the pesticide rotenone and isolated dopamine producing neurons of animals that were at an early disease stage. Transcriptomic analyses revealed that gene ontologies associated with regulation of cell death and neuronal functions were significantly enriched. Moreover, the activities of the MAPK/EGFR- and TGF-β signaling pathways were enhanced, while the Wnt pathway was dampened. In order to evaluate the role of Wnt signaling for survival of dopaminergic neurons in the disease model, we rescued the reduced Wnt signaling activity by ectopic overexpression of armadillo/β-catenin. This intervention rescued the rotenone induced movement impairments in the Drosophila model. Taken together, this initial study showed a highly relevant role of Wnt signaling for dopamine producing neurons during pathogenesis in Parkinson's disease and it implies that interfering with this pathway might by a suitable therapeutic option for the future.
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http://dx.doi.org/10.1038/s41598-018-20836-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5799194PMC
February 2018

The Role of Monoaminergic Neurotransmission for Metabolic Control in the Fruit Fly .

Front Syst Neurosci 2017 22;11:60. Epub 2017 Aug 22.

Laboratory of Molecular Physiology, Department of Zoology, Kiel UniversityKiel, Germany.

Hormones control various metabolic traits comprising fat deposition or starvation resistance. Here we show that two invertebrate neurohormones, octopamine (OA) and tyramine (TA) as well as their associated receptors, had a major impact on these metabolic traits. Animals devoid of the monoamine OA develop a severe obesity phenotype. Using flies defective in the expression of receptors for OA and TA, we aimed to decipher the contributions of single receptors for these metabolic phenotypes. Whereas those animals impaired in , 2r and share the obesity phenotype of OA-deficient (-deficient) animals, the 1r, 2r deficient flies showed reduced insulin release, which is opposed to the situation found in -deficient animals. On the other hand, OAMB deficient flies were leaner than controls, implying that the regulation of this phenotype is more complex than anticipated. Other phenotypes seen in animals, such as the reduced ability to perform complex movements tasks can mainly be attributed to the 2r. Tissue-specific RNAi experiments revealed a very complex interorgan communication leading to the different metabolic phenotypes observed in OA or OA and TA-deficient flies.
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http://dx.doi.org/10.3389/fnsys.2017.00060DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5572263PMC
August 2017

Effect of Transplantation of Bone Marrow Derived Mesenchymal Stem Cells and Platelets Rich Plasma on Experimental Model of Radiation Induced Oral Mucosal Injury in Albino Rats.

Int J Dent 2017 26;2017:8634540. Epub 2017 Feb 26.

Radiation Oncology, National Cancer Institute, Cairo University, Giza, Egypt.

Normal tissue damage following radiotherapy is still a major problem in cancer treatment. Therefore, the current work aimed at exploring the possible role of systemically injected bone marrow derived mesenchymal stem cells (BM-MSCs) and/or locally injected platelet rich plasma (PRP) in ameliorating the side effects of ionizing radiation on the rat's tongue. Twelve rats served as control group (N) and 48 rats received a single radiation dose of 13 Gy to the head and neck region; then, they were equally divided into 4 experimental groups: irradiated only (C), irradiated + MSCs (S), irradiated + (PRP) (P), and combined group (PS). Animal scarification occurred in 3 and 7 days after radiation. Then, tongues were dissected and examined histologically and for expression of bcl-2 by RT-PCR. Histological examination of the treated groups (S), (P), and (PS) revealed an obvious improvement in the histological structure of the tongue, compared to group (C), in addition to upregulated expression of bcl-2, indicating decreased apoptotic activity. . BM-MSCs and PRP have shown positive effect in minimizing the epithelial atrophy of normal oral mucosa after regional radiotherapy, which was emphasized by decreasing apoptotic activity in these tissues. Nevertheless, combined use of BM-MSCs and PRP did not reveal the assumed synergetic effect in oral tissue protection.
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http://dx.doi.org/10.1155/2017/8634540DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5346393PMC
February 2017

Expression analysis of octopamine and tyramine receptors in Drosophila.

Cell Tissue Res 2015 Sep 7;361(3):669-84. Epub 2015 Mar 7.

Zoological Institute, Molecular Physiology, Christian-Albrechts University Kiel, Kiel, Germany.

The monoamines octopamine and tyramine, which are the invertebrate counterparts of epinephrine and norepinephrine, transmit their action through sets of G protein-coupled receptors. Four different octopamine receptors (Oamb, Octß1R, Octß2R, Octß3R) and 3 different tyramine receptors (TyrR, TyrRII, TyrRIII) are present in the fruit fly Drosophila melanogaster. Utilizing the presumptive promoter regions of all 7 octopamine and tyramine receptors, the Gal4/UAS system is utilized to elucidate their complete expression pattern in larvae as well as in adult flies. All these receptors show strong expression in the nervous system but their exact expression patterns vary substantially. Common to all octopamine and tyramine receptors is their expression in mushroom bodies, centers for learning and memory in insects. Outside the central nervous system, the differences in the expression patterns are more conspicuous. However, four of them are present in the tracheal system, where they show different regional preferences within this organ. On the other hand, TyrR appears to be the only receptor present in the heart muscles and TyrRII the only one expressed in oenocytes. Skeletal muscles express octß2R, Oamb and TyrRIII, with octß2R being present in almost all larval muscles. Taken together, this study provides comprehensive information about the sites of expression of all octopamine and tyramine receptors in the fruit fly, thus facilitating future research in the field.
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http://dx.doi.org/10.1007/s00441-015-2137-4DOI Listing
September 2015

The octopamine receptor octß2R is essential for ovulation and fertilization in the fruit fly Drosophila melanogaster.

Arch Insect Biochem Physiol 2015 Mar 29;88(3):168-78. Epub 2014 Oct 29.

Christian-Albrechts University Kiel, Zoological Institute, Molecular Physiology, Kiel, Germany.

The biogenic monoamine octopamine is essential for ovulation and fertilization in insects. Release of this hormone from neurons in the thoracoabdominal ganglion triggers ovulation and sperm release from the spermathecae. Here we show that the effects of octopamine on ovulation are mediated by at least two different octopamine receptors. In addition to the Oamb receptor that is present in the epithelium of the oviduct, the octß2R receptor is essential for ovulation and fertilization. Octß2R is widely expressed in the female reproductive tract. Most prominent is expression in the oviduct muscle and the spermathecae. Animals deficient in expression of the receptor show a severe egg-laying defect. The corresponding females have a much larger ovary that is caused by egg retention in the ovary. Moreover, the very few laid eggs are not fertilized, indicating problems in the process of sperm delivery. We assume that octß2R acts in a similar way as ß2-adrenoreceptors in smooth muscles, were activation of this receptor induces an increase in cAMP levels that lead to relaxation of the muscle. Taken together, our findings show that octopaminergic control of ovulation and fertilization is more complex than anticipated and that various receptors located in different cells act together to enable a well-orchestrated activity of the female reproductive system in response to copulation.
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http://dx.doi.org/10.1002/arch.21211DOI Listing
March 2015

Transcriptional regionalization of the fruit fly's airway epithelium.

PLoS One 2014 14;9(7):e102534. Epub 2014 Jul 14.

University of Kiel, Dept. Molecular Physiology, Kiel, Germany; German Center for Lung Research (DZL), Airway Research Center North (ARCN), Germany.

Although airway epithelia are primarily devoted to gas exchange, they have to fulfil a number of different tasks including organ maintenance and the epithelial immune response to fight airborne pathogens. These different tasks are at least partially accomplished by specialized cell types in the epithelium. In addition, a proximal to distal gradient mirroring the transition from airflow conduction to real gas exchange, is also operative. We analysed the airway system of larval Drosophila melanogaster with respect to region-specific expression in the proximal to distal axis. The larval airway system is made of epithelial cells only. We found differential expression between major trunks of the airways and more distal ones comprising primary, secondary and terminal ones. A more detailed analysis was performed using DNA-microarray analysis to identify cohorts of genes that are either predominantly expressed in the dorsal trunks or in the primary/secondary/terminal branches of the airways. Among these differentially expressed genes are especially those involved in signal transduction. Wnt-signalling associated genes for example are predominantly found in secondary/terminal airways. In addition, some G-protein coupled receptors are differentially expressed between both regions of the airways, exemplified by those activated by octopamine or tyramine, the invertebrate counterparts of epinephrine and norepinephrine. Whereas the OAMB is predominantly found in terminal airway regions, the oct3βR has higher expression levels in dorsal trunks. In addition, we observed a significant association of both, genes predominantly expressed in dorsal trunks or in primary to terminal branches branches with those regulated by hypoxia. Taken together, this observed differential expression is indicative for a proximal to distal transcriptional regionalization presumably reflecting functional differences in these parts of the fly's airway system.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0102534PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4097054PMC
January 2016

Effect of 60 minutes exposure to electromagnetic field on fecundity, learning and memory, speed of movement and whole body protein of the fruit fly Drosophila melanogaster.

J Egypt Soc Parasitol 2012 Dec;42(3):639-48

Department of Zoology, Faculty of Science, Tanta University, Tanta 31527, Egypt.

This study investigated the effect of four different electrical devices as source of electromagnetic field on fecundity, learning and memory function, speed of movement, in addition to the whole body proteins of the fruit fly Drosophila melanogaster. The results showed that exposure to EMF has no significant effect on adult fecundity (ANOVA and Duncan's test) but alters learning and memory function in Drosophila larvae, especially those exposed to mobile phone. Highly significant differences occurred in the larval speed of movement after exposure to EMF, with maximal effect occurred for larvae exposed to mobile phone (their speed of movement increased 2.5 times of wild type). Some protein bands serve as characters for exposure to certain electrical devices which suggest that exposure to EMF may affect the whole body proteins.
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http://dx.doi.org/10.12816/0006347DOI Listing
December 2012
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