Publications by authors named "Samaneh Mirzaei"

20 Publications

  • Page 1 of 1

Design and psychometric evaluation of schools' resilience tool in Emergencies and disasters: A mixed-method.

PLoS One 2021 22;16(7):e0253906. Epub 2021 Jul 22.

Department of Anesthesiology and Critical Care, Shahid Rahnemoun Hospital, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Background: In addition to their educational role, resilient schools have a good capacity in response to disasters. Due to the large student population, the schools can be a safe and secure environment during disasters, in addition to maintaining their performance after. Given the role and importance of the schools, the impact of culture and environment on resilience, without any indigenous and comprehensive tool for measuring the resilience in Iran, the study aimed to design and psychometrically evaluate the measurement tools.

Method: This study was conducted using a mixed-method sequential explanatory approach. The research was conducted in two main phases of production on items based on hybrid model and the psychometric evaluation of the tool. The second phase included validity (formal, content and construction) and reliability (multiplex internal similarity, consistency and reliability).

Result: The integration of systematic and qualitative steps resulted in entering 91 items into the pool of items. After formal and content validity, 73 items remained and 44 were omitted in exploratory factor analysis. A questionnaire with 5 factors explained 52.08% of total variance. Finally, after the confirmatory factor analysis, the questionnaire was extracted with 29 questions and 5 factors including "functional", "architectural", "equipment", "education" and "safety". Internal similarity and stability in all factors were evaluated as good.

Conclusion: The result showed that the 29-item questionnaire of school resilience in emergencies and disasters is valid and reliable, that can be used to evaluate school resilience. On the other hand, the questionnaire on assessment of school resilience in disasters enables intervention to improve its capacity.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0253906PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8297909PMC
July 2021

The effect of implementation of evidence-based eye care protocol for patients in the intensive care units on superficial eye disorders.

BMC Ophthalmol 2021 Jul 13;21(1):275. Epub 2021 Jul 13.

Department of Nursing, Research Centre for Nursing and Midwifery Care, Mother and Newbern Health Research Centre, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Background: Superficial eye disorders are one of the most common complications of improper eye care in intensive care units that can lead to corneal ulcers and permanent eye damage. The aim of this study was to determine the effect of the implementation of eye care protocol on the incidence of infection and superficial eye disorders in patients admitted to intensive care units.

Methods: This study was a cross-over clinical trial that was performed on 32 patients admitted to the intensive care unit with reduced or no blink reflex following loss of consciousness or receiving sedatives. The eye of the test group received eye care according to the protocol and the eye of the control group received the routine care of the ward. The data collection form included demographic and clinical information and the clinical score scale of superficial eye disorders, which were completed in 7 days for both eyes. Data analysis was performed by McNemar and Cochran tests with a 95 % confidence interval.

Results: In the study of superficial eye disorders, the frequency of dacryorrhea and hyperemia was not significantly different in the second to seventh days in the control and test eyes (P < 0.05). The frequency of xerophthalmia was not significantly different between the control and the test eyes on the second to third days (P < 0.05), but there was a significant difference on the fourth, fifth, sixth, and seventh days (P = 0.0001). Also, the frequency of corneal opacity was not significantly different in the second and third days (P < 0.05), but in the fourth (P < 0.05), fifth, sixth, and seventh days, this difference was significant (P = 0.0001).

Conclusions: Based on the results, although the implementation of eye care protocol has been able to have a significant effect on reducing ocular complications and problems, routine eye care in the intensive care unit also has clinical effectiveness. Therefore, in order to prevent and completely eliminate eye disorders in the intensive care unit, more evidence and research are needed.

Trial Registration: The trial was retrospectively registered on https://en.irct.ir/trial/43493 on 13 November 2019 (13.11.2019) with registration number [IRCT20140307016870N5].
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http://dx.doi.org/10.1186/s12886-021-02034-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8276465PMC
July 2021

Effect of Training Eye Care Clinical Guideline for ICU Patients on Clinical Competence of Eye Care in Nurses.

Crit Care Res Pract 2021 12;2021:6669538. Epub 2021 Jan 12.

Nursing Department, Nursing and Midwifery Research Center, Maternal and Newborn Health Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Introduction: Sight is one of the most important and vital human senses. Lack of proper eye care (EC) in anesthetized patients can lead to serious ocular complications and even vision loss. Insufficient knowledge, attitude, and skills of nurses are considered as a barrier to providing EC in the intensive care unit (ICU). The aim of the present study was to determine the effect of training EC clinical practice guidelines for ICU patients on nurses' knowledge, attitude, and practice of EC.

Methods: This was an interventional study with a pre-post design performed on 60 ICU nurses. For the experimental group, EC clinical guideline training was performed for anesthetized patients in three sessions. The data collection tool included nurses' clinical competence of the EC questionnaire with a possible score range of 0-86. This tool consists of three domains, including knowledge (0-18), attitude (0-28), and practice (0-40), which was completed in a self-assessment manner before and three months after the training program. Data analysis was carried out using SPSS16. . The mean scores of knowledge, attitude, and practice after the intervention in the experimental and control groups were 15.03 ± 2.72 and 11.11 ± 3.50, 25.65 ± 3.47 and 22.07 ± 3.08, and 33.88 ± 4.14 and 28.5 ± 55.08, respectively, which were statistically significant ( ≤ 0.001). Also, the total score of clinical competence of EC after the intervention in the experimental and control groups was 74.56 ± 7.93 and 61.74 ± 9.66, which showed a significant difference ( ≤ 0.001).

Conclusion: Training nurses based on EC clinical guidelines for anesthetized patients can improve the knowledge, attitude, and practice of ICU nurses. Evidence-based EC practice requires continuous training based on clinical guidelines and EC practice monitoring by nursing managers according to EC clinical guideline for an anesthetized patient.
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http://dx.doi.org/10.1155/2021/6669538DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7817315PMC
January 2021

Operational Strategies for Establishing Disaster-Resilient Schools: A Qualitative Study.

Adv J Emerg Med 2020 1;4(2):e23. Epub 2019 Sep 1.

Department of Civil Engineering, Yazd Branch, Islamic Azad University, Yazd, Iran.

Introduction: Resilient schools can warranty students' health and survival at disasters. It is obligatory that schools be prepared for natural challenges through local programs. Considering the great population of students, disaster-resilient schools can be a safe and suitable environment for students at the time of disaster.

Objective: This study aims to identify certain operational strategies for establishing schools resilient to natural disasters.

Method: This qualitative study was based on conventional content analysis. Using purposive sampling method, 24 experts in the fields of health in disasters, construction engineering, psychology, teaching, and administrative management participated in the study. Maximum variation sampling continued until data saturation was achieved. The data collected via unstructured interviews were analyzed with Graneheim and Lundmen's conventional content analysis.

Results: Content analysis resulted in four main categories as operational strategies for establishing disaster-resilient schools including: 1) "construction and non-construction optimization", with four subcategories of construct risk management, optimization of construct architecture and physical structure, correct construct localization, and promotion of non-construct safety, 2) "promotion of organizational coordination and interactions" with two subcategories, namely improvement in intra-organizational communication and improvement in extra-organizational communication, 3) "improvement in education" with three subcategories of holding educational courses for families and students, holding educational courses for managers and personnel, and holding simulated exercises, and 4) "process promotion" with four subcategories of increased preparedness, correct planning, creation of organizational structure, and rehabilitation facilitation.

Conclusion: Various factors affecting schools' response to disasters form operational strategies to establish disaster-resilient schools. These strategies influence pre- and post-disaster preparedness. Awareness of these components followed by preparedness prior to disasters can save students' lives, improve school performance after disasters, and aid in establishing disaster-resilient schools as safe lodgings.
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http://dx.doi.org/10.22114/ajem.v0i0.241DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7163258PMC
September 2019

Institutional Accreditation in Medical Education: The Experience of The Survey Visit Teams.

J Educ Health Promot 2020 28;9:39. Epub 2020 Feb 28.

Associate Professor, Medical Education Department, Medical Education Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.

Objectives: This study discussed about challenges and opportunities of institutional accreditation in Iranian medical universities. The lesson learned of the first round of the accreditation would direct initiatives and solutions for future accreditation.

Methodology: This research is a qualitative content analysis research that studied the experience of the survey visit teams. Semistructured interviews with a purposive sampling approach were done to reach data saturation. Constant comparative method was used to analyze the data.

Results: The emergent themes were incompatible scheduling with accreditation workload, accreditation sustainability, the weakness of the survey instrument, advantage of the survey instrument, the quality of evaluators' survey, the opportunity of progress for universities, accreditation perspective, defendable cost opportunity, perceived injustice, and the leading strategies. In this study, the relationship between categories and themes was shown with a systematic approach.

Conclusion: In medical education systems, it is necessary to continue the research and development studies using the findings in each accreditation period to achieve the prospect of international accreditation.
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http://dx.doi.org/10.4103/jehp.jehp_518_19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161652PMC
February 2020

Assessment of school resilience in disasters: A cross-sectional study.

J Educ Health Promot 2020 30;9:15. Epub 2020 Jan 30.

Department of Civil Engineering, Yazd Branch, Islamic Azad University, Yazd, Iran.

Background And Objectives: School resilience is defined as risk-reducing strategies used to create a safe environment for students when faced natural disasters. Resilient schools, in addition to their educational role, provide a suitable capacity for responding to disasters and rehabilitation after the incidence. This study determined the level of disaster resilience of schools in Yazd, central Iran.

Materials And Methods: This is a descriptive-analytic study conducted among 400 schools and 367 participants in Yazd, 2018. To collect data, we used the school resilience in disasters questionnaire (α =0.95 and intraclass correlation coefficient = 0.97 [95% confidence interval: 0.96-0.98]) containing 48 questions. We also analyzed the gleaned data through the Pearson correlation coefficient, one-way ANOVA, and independent -test.

Results: The total score of school disaster resilience was 153.30 ± 29.57. In these schools, the function had the highest (47.76 ± 13.96), and safety had the lowest (6.74 ± 3.18) score among all areas of school disaster resilience. There was a positive significant correlation between total resilience and areas of function, education, structural, nonstructural, architecture, commute routes, safety, location, and equipment ( < 0.001). Location had the smallest ( = 0.424) and function had the greatest ( = 0.854) correlation with total resilience.

Conclusion: It can help the school management board in assessing the level of resilience of their school and determining the priorities for disaster risk reduction. Awareness of the status of resilience can help policy-makers and experts create an effective program for increasing resilience.
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http://dx.doi.org/10.4103/jehp.jehp_389_19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7034159PMC
January 2020

Cost-Effective Double-Layer Hydrogel Composites for Wound Dressing Applications.

Polymers (Basel) 2018 Mar 12;10(3). Epub 2018 Mar 12.

Centre for NanoScale Science and Technology, College of Science and Engineering, Flinders University, Tonsley, SA 5042, Australia.

Although poly vinyl alcohol-poly acrylic acid (PVA-PAA) composites have been widely used for biomedical applications, their incorporation into double-layer assembled thin films has been limited because the interfacial binding materials negatively influence the water uptake capacity of PVA. To minimize the effect of interfacial binding, a simple method for the fabrication of a double-layered PVA-PAA hydrogel was introduced, and its biomedical properties were evaluated in this study. Our results revealed that the addition of PAA layers on the surface of PVA significantly increased the swelling properties. Compared to PVA, the equilibrium swelling ratio of the PVA-PAA hydrogel increased ( = 0.035) and its water vapour permeability significantly decreased ( = 0.04). Statistical analysis revealed that an increase in pH value from 7 to 10 as well as the addition of PAA at pH = 7 significantly increased the adhesion force ( < 0.04). The mechanical properties-including ultimate tensile strength, modulus, and elongation at break-remained approximately untouched compared to PVA. A significant increase in biocompatibility was found after day 7 ( = 0.016). A higher release rate for tetracycline was found at pH = 8 compared to neutral pH.
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http://dx.doi.org/10.3390/polym10030305DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6415111PMC
March 2018

Enhancement of osteogenic differentiation of adipose-derived stem cells by PRP modified nanofibrous scaffold.

Cytotechnology 2018 Dec 6;70(6):1487-1498. Epub 2018 Aug 6.

Stem Cell Technology Research Center, Tehran, Iran.

Recent developments in bone tissue engineering have paved the way for more efficient and cost-effective strategies. Additionally, utilization of autologous sources has been considered very desirable and is increasingly growing. Recently, activated platelet rich plasma (PRP) has been widely used in the field of bone tissue engineering, since it harbours a huge number of growth factors that can enhance osteogenesis and bone regeneration. In the present study, the osteogenic effects of PRP coated nanofibrous PES/PVA scaffolds on adipose-derived mesenchymal stem cells have been investigated. Common osteogenic markers were assayed by real time PCR. Alkaline phosphate activity, calcium deposition and Alizarin red staining assays were performed as well. The results revealed that the highest osteogenic differentiation occurred when cells were cultured on PRP coated PES/PVA scaffolds. Interestingly, direct application of PRP to culture media had no additive effects on osteogenesis of cells cultured on PRP coated PES/PVA scaffolds or those receiving typical osteogenic factors. The highest osteogenic effects were achieved by the simplest and most cost-effective method, i.e. merely by using PRP coated scaffolds. PRP coated PES/PVA scaffolds can maximally induce osteogenesis with no need for extrinsic factors. The major contribution of this paper to the current researches on bone regeneration is to suggest an easy, cost-effective approach to enhance osteogenesis via PRP coated scaffolds, with no additional external growth factors.
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http://dx.doi.org/10.1007/s10616-018-0226-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269372PMC
December 2018

The effect of nanofibre-based polyethersulfone (PES) scaffold on the chondrogenesis of human induced pluripotent stem cells.

Artif Cells Nanomed Biotechnol 2018 Dec 6;46(8):1948-1956. Epub 2017 Nov 6.

e Cellular and Molecular Biology Research Center , Shahid Beheshti University of Medical Sciences , Tehran , Iran.

Human induced pluripotent stem cells (iPSCs) have been shown to have promising potential for regenerative medicine and tissue engineering applications. Chondrogenic differentiation of iPSCs is important for application in cartilage tissue engineering. In this study, we considered the effect of nanofibre-based polyethersulfone (PES) scaffold on the chondrogenesis of iPSCs. IPSC cells were cultured on the PES scaffold and scaffold free method. After 21 d, real-time PCR was performed to evaluate the cartilage-specific genes in the mRNA levels. For confirm our results, we have done immunocytochemistry and scanning electron microscopy (SEM) imaging. According to the results, higher significant expressions of common chondrogenic-related genes such as aggrecan, collagen type II and collagen type X were observed in PES seeded human iPSCs when compared to the mRNA levels measured in scaffold free method. Expression of collagen type I down regulated in both methods. Also, both methods were showed a similar pattern of expression of SOX9. Our results showed that nanofibre-based PES scaffold enhanced the chondrogenesis of iPSCs and the highest capacity for differentiation into chondrocyte-like cells. These cells and PES scaffold were demonstrated to have great efficiency for treatment of cartilage damages and lesions.
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http://dx.doi.org/10.1080/21691401.2017.1396998DOI Listing
December 2018

Natural Compounds for Skin Tissue Engineering by Electrospinning of Nylon-Beta Vulgaris.

ASAIO J 2018 Mar/Apr;64(2):261-269

Natural compounds containing polysaccharide ingredients have been employed as candidates for treatment of skin tissue. Herein, for the first time, electrospinning setup was proposed to fabricate an efficient composite nanofibrous structure of Beta vulgaris (obtained from Beet [Chenopodiaceae or Amaranthaceae]) belonged to polysaccharides and an elastic polymer named nylon 66 for skin tissue engineering. Both prepared scaffolds including noncomposite and composite types were studied by Scanning electron microscope (SEM), Fourier transform infrared (FTIR) spectroscopy, mechanical assay, and contact angle. Scanning electron microscope examinations have approved the uniform and homogeneous structure of composite nanofibers containing nylon polymer and B. vulgaris extract. FTIR spectroscopy was endorsed the presence of B. vulgaris extract within the interwoven mat of nanofibers. Also, measurement of mechanical property with cell-laden composite scaffolds approved the desirable similarity between corresponding scaffold and native skin tissue. To our surprise, it was found that compared with nylon nanofibrous scaffold, composite sample containing B. vulgaris extract has lower contact angle indicating a higher hydrophilic surface. After cell seeding process of keratinocyte cells on composite and noncomposite scaffolds, SEM and 3[4,5-dimethylthiazoyl-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assays approved higher number of attached cells onto the corresponding composite electrospun membrane. Epidermal gene expression such as involucrin, cytokeratin 10, and cytokeratin 14 was observed through real-time polymerase chain reaction (PCR) technique. Furthermore, immunocytochemistry results (cytokeratin 10 and loricrin) approved that the original property of keratinocytes was strongly preserved using composite scaffold. The corresponding study tries to introduce a new type of natural-based scaffolds for dermal tissue engineering that exhibits an elastic behavior similar to native skin tissue.
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http://dx.doi.org/10.1097/MAT.0000000000000611DOI Listing
March 2019

Different Porosities of Chitosan Can Influence the Osteogenic Differentiation Potential of Stem Cells.

J Cell Biochem 2018 01 17;119(1):625-633. Epub 2017 Jul 17.

Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Scaffolds porosity has an important role in in vitro and in vivo differentiation process of stem cells with given the amount of space available to the cells to proliferate and differentiate. In the present study, chitosan with three porosities including 10%, 15%, and 20% that created by gelatin were used for investigation of the proliferation and osteogenic differentiation potential of adipose-derived stem cells (ADSCs). In order to be more like the scaffold to natural bone tissue, freeze-drying method was used in the scaffold preparation. Scaffold morphology, cell attachment, and toxicity were evaluated using scanning electron microscopy and MTT assay. Then, osteogenic differentiation potential of ADSCs cultured on chitosan with different porosities was evaluated by common osteogenic markers such as Alizarin red staining, ALP activity, calcium content, and osteogenic-related genes expression via real-time RT-PCR. Although all scaffolds supported the proliferation and differentiation of ADSCs, but 10% scaffold demonstrated higher amount of osteogenic markers in comparison with the other porosities and control groups. Taking together, it can be concluded that osteogenic differentiation well done in the scaffolds with lower porosity because density of the cells will increase by forcing resulted from the scaffold, so osteogenic differentiation of the stem cells have an inverse association with scaffold porosity. J. Cell. Biochem. 119: 625-633, 2018. © 2017 Wiley Periodicals, Inc.
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http://dx.doi.org/10.1002/jcb.26223DOI Listing
January 2018

Inactive Hepatitis B Carrier and Pregnancy Outcomes: A Systematic Review and Meta-analysis.

Iran J Public Health 2017 Apr;46(4):468-474

Dept. of Reproductive Health, Student Research Committee, School of Nursing and Midwifery, Shahroud University of Medical Sciences, Shahroud, Iran.

Background: We aimed to explore whether maternal asymptomatic hepatitis B (HB) infection effects on pre-term rupture of membranous (PROM), stillbirth, preeclampsia, eclampsia, gestational hypertension, or antepartum hemorrhage.

Methods: We searched the PubMed, Scopus, and ISI web of science from 1990 to Feb 2015. In addition, electronic literature searches supplemented by searching the gray literature (e.g., conference abstracts thesis and the result of technical reports) and scanning the reference lists of included studies and relevant systematic reviews. We explored statistical heterogeneity using the, I2 and tau-squared (Tau2) statistical tests.

Results: Eighteen studies were included. Preterm rupture of membranous (PROM), stillbirth, preeclampsia, eclampsia, gestational hypertension and antepartum hemorrhage were considerable outcomes in this survey. The results showed no significant association between inactive HB and these complications in pregnancy. The small amounts of -value and chi-square and large amount of I2 suggested the probable heterogeneity in this part, which we tried to modify with statistical methods such as subgroup analysis.

Conclusion: Inactive HB infection did not increase the risk of adversely mentioned outcomes in this study. Further, well-designed studies should be performed to confirm the results.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5439035PMC
April 2017

Enhanced chondrogenic differentiation of stem cells using an optimized electrospun nanofibrous PLLA/PEG scaffolds loaded with glucosamine.

J Biomed Mater Res A 2017 Sep 6;105(9):2461-2474. Epub 2017 Jun 6.

Stem Cell Technology Research Center, Tehran, 1997775555, Iran.

Recently, tissue engineering has become one of the most important approaches in medical research for the treatment of injuries and lesions. In the present study, poly(l-lactide) acid (PLLA), and polyethylene glycol (PEG) with different ratios and PEG molecular weights were used in order to produce appropriate nanofibrous scaffolds using the electrospinning technique for cartilage tissue engineering applications. Glucosamine was also incorporated into the polymeric scaffolds to enhance the biological properties. Mesenchymal stem cells and chondrocytes were used to monitor the differentiation yield. Water absorption test, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, real-time reverse transcription polymerase chain reaction and mechanical properties analyses were performed to characterize the prepared scaffolds. All fabricated scaffolds had porous and nanofibrous structure with interconnected pores. The PLLA-PEG scaffolds containing PEG with the molecular weights of 3000 and 20,000 and ratio of 85:15 was selected for glucosamine incorporation and cell culture studies due to their superior mechanical properties. According to our data, it was identified that PLLA/PEG 20,000 containing glucosamine had the most capability to support protein absorption, stem cell attachment, and proliferation. Chondrogenic-related genes such as sex-determining region Y-Box 9 (SOX9) and collagen type II were shown to be expressed on these scaffolds higher than those observed on control groups. Taking together, it was demonstrated that in combination with PLLA, PEG 20,000 is a suitable substrate to improve the mechanical and physical properties of nanofibrous scaffolds. In addition, glucosamine-PLLA/PEG 20,000 was shown to support stem cell attachment, proliferation, and chondrogenic differentiation and so holds promising potential for cartilage tissue engineering applications. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2461-2474, 2017.
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http://dx.doi.org/10.1002/jbm.a.36104DOI Listing
September 2017

Mesalazine Has No Effect on Mucosal Immune Biomarkers in Patients with Diarrhea-Dominant Irritable Bowel Syndrome Referred to Shariati Hospital: A Randomized Double-Blind, Placebo-Controlled Trial.

Middle East J Dig Dis 2017 Jan;9(1):20-25

Associate Professor, Digestive Disease Research Institute, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.

BACKGROUND Intestinal mast cells may cause gastrointestinal symptoms in patients with diarrhea-dominant irritable bowel syndrome (IBS). The objective of this study was to determine the effect of mesalazine on the number of lamina propria mast cells and clinical manifestations of patients with diarrhea-dominant IBS referred to Shariati Hospital affiliated to Tehran University of Medical Sciences. METHODS This was a randomized placebo-controlled double-blind trial conducted on 49 patients with diarrhea-dominant IBS. The patients were randomly assigned to one of the experiment or control groups. The patients in experiment group took 2400 mg mesalazine daily in three divided doses for 8 weeks and the patient in control group took placebo on the same basis. Our first targeted outcome was an assigned downturn of mast cells number to the safe colonic baseline and the next one was a marked palliation of disease symptoms. Data were analyzed conforming intention-to-treat method. We used MANCOVA test to compare our both assigned outcomes in the two groups. We also compared the data with baseline values in both groups.All statistical tests were performed at the significance level of 0.05. RESULTS There was no significant difference between Mesalazine and placebo groups regarding the number of mast cells ( value=0.396), abdominal pain ( value=0.054), bloating ( value=0.365), defecation urgency ( value=0.212), and defecation frequency ( value=0.702). CONCLUSION Mesalazine had no significant effect either on the number of mast cells or on the severity of disease symptoms. This finding seems to be inconsistent with the hypothesis indicating immune mechanisms as potential therapeutic targets in IBS. The possible difference in this effect of Mesalazine should be evaluated in further studies among populations varying in race, ethnic, and geographical characteristics.
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http://dx.doi.org/10.15171/mejdd.2016.47DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5308130PMC
January 2017

The Effect of Renal Transplantation on Respiratory Muscle Strength in Patients with End Stage Renal Disease.

Tanaffos 2016 ;15(2):83-88

Department of Internal Medicine, Pulmonology Ward, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Background: There is evidence of musculoskeletal and respiratory involvement in patients with chronic kidney disease (CKD). This is attributed to protein calorie imbalance that is caused by the disease process, and hemodialysis and is generally referred to as uremic myopathy. This results in calcification of respiratory muscles such as diaphragm and intercostal muscles. There are limited data about respiratory muscle strength in patients with CKD. We intended to evaluate the effect of kidney transplantation on respiratory muscles strength in patients with CKD.

Materials And Methods: Spirometry was used to measure maximal inspiratory mouth pressure (P), which was calculated by using the lung residual volume and maximal respiratory pressures at the mouth (P), 2 days before and again 30 days after kidney transplantation in 26 patients with ESRD. PIMAX and PEMAX values less than 60% of the predicted value were considered abnormal.

Results: Mean ± SD PIMAX values showed significant increase from 31.88 ± 8.58 cmH2O before kidney transplantation to 37.65 ± 13.39 cmH2O after transplant (P < 0.001). Similarly, a significant increase in PEMAX values was observed from 33.04 ± 16.12 cmH2O to 39.19 ± 20.34 cmH2O (P < 0.001). Nineteen patients (73.1%) showed significant increases in PIMAX and PEMAX values. Mean serum creatinine decreased from 6.94 to 1.32 (P < 0.001) after transplant.

Conclusion: Although both PIMAX and PEMAX values increased significantly after kidney transplant, these measurements were still below lower limit of normal. This suggests that factors other than uremic myopathy may contribute to respiratory muscle weakness in patients CKD.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5127619PMC
January 2016

Hepatogenic Differentiation of Human Induced Pluripotent Stem cells on Collagen-Coated Polyethersulfone Nanofibers.

ASAIO J 2017 May/Jun;63(3):316-323

From the *Stem Cell and Regenerative Medicine, Institute of Medical Biotechnology, National Institute of Genetic Engineering & Biotechnology (NIGEB), Tehran, Iran; †Department of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical sciences, Tehran, Iran; ‡Department of Hematology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran; and §Stem Cell Technology Research Center, Tehran, Iran.

Many scientists have been fascinated with induced pluripotent stem cells (iPSCs) for cell replacement therapies. Nanofibrous biocompatible scaffolds have been shown to foster better cell adhesion and improve stem cell differentiation. In the current study, after fabrication using electrospinning technique and surface modifications, the characteristics of polyethersulfone (PES) nanofibers were determined by scanning electron microscopy, attenuated total reflection Fourier transform infrared spectroscopy, and 3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyltetrazolium bromide (MTT) assay. Then, the hepatogenic potential of iPSCs was evaluated using real-time reverse transcription polymerase chain reaction (RT-PCR) and immunocytochemistry (ICC) after culture on collagen-coated polyethersulfone (PES/COL) scaffolds. After scaffolds characterization, analysis of two important definitive endoderm specific markers (Sox17 and Foxa2) using real-time RT-PCR and ICC indicated increase in their mRNA and protein levels after 5 days of hepatogenic induction. In addition, to determine hepatic differentiation of iPSCs cultured on PES/COL, the expression of albumin and α-fetoprotein was evaluated by ICC after 20 days. Real-time RT-PCR analysis showed increased expression of albumin, TAT, cytokeratin 19, and Cyp7A1 genes during the course of differentiation program. Finally, enzyme-linked immunosorbent assay analysis demonstrated an increased expression of albumin in the protein level after 28 days of differentiation. In conclusion, our results demonstrated that PES/COL nanofibrous scaffolds could be a proper substrate to significantly increase the hepatogenic differentiation potential of iPSCs and could also be introduced as a promising candidate for liver tissue engineering applications.
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http://dx.doi.org/10.1097/MAT.0000000000000469DOI Listing
January 2018

Predictive factors of restless leg syndrome in hemodialysis patients.

J Renal Inj Prev 2016 20;5(2):89-93. Epub 2016 May 20.

Department of Nursing, School of Nursing and Midwifery, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.

Introduction: The restless leg syndrome (RLS) is a neurologic disorder suffering the hemodialysis patients. Although the pathophysiology of this syndrome remains unknown yet, an investigation of the parameters pertinent to it may help to develop the related medical knowledge and to improve the therapeutic-care interventions in this regard.

Objectives: The correlation between the RLSs on individual, clinical, and laboratory indices in patients undergoing hemodialysis.

Patients And Methods: This descriptive-analytic study was conducted on 104 hemodialysis patients. Diagnosis of RLS was made using the International RLS Standard Questionnaire. The data on individual, clinical, and laboratory indices were obtained from patients' recorded files and interviews.

Results: Based on our findings, 28.8% of the patients undergoing hemodialysis were affected with mild RLS, 41.7% with moderate RLS, and 29.5% with severe RLS. There was a statistically significant correlation between affliction with RLS on the one hand, and age and gender, on the other (P < 0.05). However, there was no significant correlation between RLS and education level, occupation, length of hemodialysis, fasting blood sugar (FBS), hepatitis B and C, serum blood urea nitrogen (BUN), creatinine (Cr), iron, hemoglobin (Hb) level and also KT/Vor URR (P < 0.05).

Conclusion: Regarding the high prevalence of RLS among the hemodialysis patients, there is the necessity for taking more care of these patients to reduce the somatic complications of the RLS especially among the elderly and female patients and to control the blood sugar of these patients at the normal level.
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http://dx.doi.org/10.15171/jrip.2016.19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4962676PMC
July 2016

Right Ventricular Thrombus and Cerebral Artery Aneurysm in a Patient with Behçet's Disease.

Sultan Qaboos Univ Med J 2016 May 15;16(2):e250-3. Epub 2016 May 15.

Preventive Cardiovascular Research Centre Kermanshah, Kermanshah University of Medical Sciences, Kermanshah, Iran.

We report a 35-year-old woman referred to the Imam Ali Hospital, Kermanshah, Iran, in July 2014 for evaluation of postoperative dyspnoea after neurosurgery performed seven days previously for a ruptured cerebral artery aneurysm. She was known to have Behçet's disease with a history of recurrent oral and genital aphthous ulcers and uveitis. At referral, her symptoms included vertigo, dysarthria, palpitations and chest pain. Transthoracic echocardiography (TTE) revealed a large thrombus in her right ventricle outflow tract and open-heart surgery was performed eight days after the previous surgery to remove the clot. The postoperative period was complicated by transient acute renal failure, which resolved spontaneously. The patient was discharged 13 days after the cardiac surgery on warfarin, prednisolone, azathioprine and cyclophosphamide. Cyclophosphamide and azathioprine were discontinued after three months as the symptoms had completely resolved; however, prednisolone was continued due to recurrent uveitis. A 10-month follow-up TTE scan revealed no thrombus recurrence and treatment with warfarin and prednisolone was continued.
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http://dx.doi.org/10.18295/squmj.2016.16.02.020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4868529PMC
May 2016

Chronic hepatitis B infection is not associated with increased risk of vascular mortality while having an association with metabolic syndrome.

J Med Virol 2016 Jul 15;88(7):1230-7. Epub 2016 Jan 15.

Liver and Pancreatobiliary Diseases Research Center, Digestive Disease Research Institute, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.

This study aimed to assess the association of chronic hepatitis B (CHB) with vascular mortality and metabolic syndrome (MS) using data from a large population-based cohort study in Iran. A total of 12,781 participants (2249 treatment-naïve CHB and 10,532 without CHB) were studied. Logistic regression model was used to assess the association between MS and CHB with adjustment for age, ALT, PLT, alcohol intake, smoking, exercise, and socioeconomic status. MS was defined according to the ATPIII guidelines. Cox proportional hazards model was used to assess the hazard ratios for overall and vascular related mortality. There was a significant association between CHB infection and overall mortality (hazard ratio (95%CI) of 1.44 (1.16-1.79), P < 0.001) after adjusting for other confounders. However, we found no association between CHB infection and mortality from vascular events (hazard ratio (95%CI) of 1.31 (0.93-1.84), P = 0.124) even after subgroup analysis by ALT. Furthermore, increased risk of overall mortality in CHB infected individuals was not related to MS and vice versa (P for interaction = 0.06). We noted a significant direct association between CHB infection and MS in women (OR (95%CI); 1.23 (1.07-1.42), P < 0.004). However, CHB was inversely associated with MS in men (OR (95%CI), 0.85 (0.79-0.99). This gender dependent association was related to high BP levels in women. In this study no association between CHB infection and mortality from vascular events was found. Further longitudinal studies should be done to investigate the exact impact of HBV infection on metabolic parameters and vascular pathology.
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http://dx.doi.org/10.1002/jmv.24466DOI Listing
July 2016

Evaluation of Complications of Heart Surgery in Children With Congenital Heart Disease at Dena Hospital of Shiraz.

Glob J Health Sci 2015 Aug 23;8(5):33-8. Epub 2015 Aug 23.

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Introduction: Today, with progress in the field of congenital heart surgery, different complicated actions are done in children. These actions may be associated with several complications, especially open heart surgery in which the cardiopulmonary bypass (CPB) is used. Serious complications can be caused high morbidity and mortality rates. Present study has been performed to determine the incidence of morbidity and mortality in cardiac surgery in children.

Method: In a cross-sectional retrospective, records of 203 patients undergoing surgery for congenital heart disease in Dena hospital during 2013-2015 were reviewed for incidence of complications. Data was analyzed by using descriptive and analytical statistics and using SPSS version 18.

Results: The mean age of samples was 3/65±4/47 years. The majority of samples (73/8%) were undergoing open surgery. The overall adverse cardiovascular complications were respectively, renal complications (44/3%), lung (40/3%), anemia (35/9%), heart (34/4%), gastrointestinal (17/2%), brain (14/2%), need for re-intubation of the trachea 11/3%), infection (7/8%) required reoperation (5/9%) and vascular complications (1/4%).

Conclusion: High incidence of complications after congenital heart surgery makes necessary attention to complications and their treatment after surgery. It is necessary to apply the measures and careful monitoring of patients to minimize these effects.
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http://dx.doi.org/10.5539/gjhs.v8n5p33DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4877226PMC
August 2015
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