Publications by authors named "Sai Zhang"

294 Publications

BiO(OH)I solid solution with rich oxygen vacancies: interlayer guest hydroxyl for improved photocatalytic properties.

J Colloid Interface Sci 2021 Jul 16;605:1-12. Epub 2021 Jul 16.

College of Urban and Rural Construction, Hebei Agricultural University, Baoding 071001, China.

A series of BiO(OH)I solid solution (SS) catalysts were successfully prepared by ion exchange of I and OH between the [BiO] layers. The morphology and microstructure were studied in depth using X-ray diffraction (XRD), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), and Brunauer-Emmett-Teller (BET) method, etc. Tunable absorption in the visible-light region was achieved by changing the proportion of OH to I. Due to the etching effect of OH, oxygen vacancies (OVs) greatly increased for the SS catalysts, and were confirmed by X-ray photoelectron spectroscopy (XPS), UV-vis diffuse reflectance spectroscopy (DRS), and electron paramagnetic spectroscopy (EPR). The unique composition of OH, I, OV, and [BiO] layers in BiO(OH)I materials resulted in diverse photoexcitations. The BiO(OH)I photocatalyst displayed a 10-fold-improved 2-chlorophenol (2-CP) degradation rate compared to BiOI. The interfacial reaction process by the photoinduced valence-band holes and conduction-band electrons proved to be a more efficient pathway for organic pollutant degradation by the BiO(OH)I SS photocatalyst. The OVs in the SS photocatalyst facilitated photoexcited and electron migration and transformation.
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http://dx.doi.org/10.1016/j.jcis.2021.07.060DOI Listing
July 2021

Epileptiform activity in mouse hippocampal slices induced by moderate changes in extracellular Mg, Ca, and K.

BMC Neurosci 2021 Jul 23;22(1):46. Epub 2021 Jul 23.

Krembil Research Institute, University Health Network, Toronto, ON, Canada.

Background: Rodent brain slices-particularly hippocampal slices-are widely used in experimental investigations of epileptiform activity. Oxygenated artificial cerebrospinal fluid (ACSF) is used to maintain slices in vitro. Physiological or standard ACSF containing 3-3.5 mM K, 1-2 mM Mg, and 1-3 mM Ca generally does not induce population epileptiform activity, which can be induced by ACSF with high K (8-10 mM), low Mg, or low Ca alone or in combination. While low-Mg ACSF without intentionally added Mg salt but with contaminating Mg (≤ 50-80 µM) from other salts can induce robust epileptiform activity in slices, it is unclear whether such epileptiform activity can be achieved using ACSF with moderately decreased Mg. To explore this issue, we examined the effects of moderately modified (m)ACSF with 0.8 mM Mg, 1.3 mM Ca, and 5.7 mM K on induction of epileptiform discharges in mouse hippocampal slices.

Results: Hippocampal slices were prepared from young (21-28 days old), middle-aged (13-14 months old), and aged (24-26 months old) C57/BL6 mice. Conventional thin (0.4 mm) and thick (0.6 mm) slices were obtained using a vibratome and pretreated with mACSF at 35-36 °C for 1 h prior to recordings. During perfusion with mACSF at 35-36 °C, spontaneous or self-sustained epileptiform field potentials following high-frequency stimulation were frequently recorded in slices pretreated with mACSF but not in those without the pretreatment. Seizure-like ictal discharges were more common in thick slices than in thin slices.

Conclusions: Prolonged exposure to mACSF by pretreatment and subsequent perfusion can induce epileptiform field potentials in mouse hippocampal slices.
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http://dx.doi.org/10.1186/s12868-021-00650-3DOI Listing
July 2021

Value of Barthel, PLAN and NIHSS scores for predicting the death of patients with acute ischemic stroke during their 5-year follow-up.

J Clin Neurosci 2021 Aug 8;90:94-98. Epub 2021 Jun 8.

Department of Neurology, The First Hospital of Hebei Medical University, Shijiazhuang, Hebei 050031, China. Electronic address:

Objective: This study aimed to investigate the value of Barthel, PLAN, and NIHSS scores for predicting death in the 5-year follow-up after patients with AIS are discharged and find a simple and convenient predictive scale.

Methods: Data were prospectively collected from 678 patients with AIS. Patients' death after 5 years of follow-up was considered the final event. The predictors of death were examined through single-factor and multivariate analysis. The receiver operating characteristic curve (ROC) of the patients' Barthel, PLAN, and NIHSS scores was drawn, and the area under the curve (AUC) was calculated. Differences in the predictive power of the three scales were compared using MedCalc. The goodness of fit between predictive and actual models was evaluated with the Hosmer-Lemeshow method.

Results: Multivariate analysis suggested that the BI score was an independent predictor of death from AIS in the 5-year follow-up. The Barthel, PLAN, and NIHSS scale scores predicted the 5-year mortality AUC values of AIS were 0.687 [95% CI, (0.649-0.722)], 0.621 [95% CI, (0.583-0.659)], 0.637 [95% CI, (0.599-0.674)], the Hosmer-Lemeshow test revealed P > 0.05, indicating that the three models had a good fit. In pairwise comparison, the AUC value of the BI score was significantly greater than that of the NIHSS scores (Pc = 0.0189). BI and PLAN scores were very close to statistical significance (Pc = 0.0513). However, PLAN and NIHSS scores had no significant differences (Pc = 1.7493).

Conclusion: Simple BI scores had a high predictive value for the death of Chinese patients with AIS within 5 years.
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http://dx.doi.org/10.1016/j.jocn.2021.05.049DOI Listing
August 2021

Identification and functional characterization of sex pheromone receptors in mirid bugs (Heteroptera: Miridae).

Insect Biochem Mol Biol 2021 Jul 4;136:103621. Epub 2021 Jul 4.

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China; Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China. Electronic address:

Mirid bugs are a group of important insect pests that cause large annual losses in agricultural production. Many studies have focused on the isolation and identification of sex pheromones in mirid bugs, and the components and biological activity of the sex pheromones have also been studied as a way to control these pests. However, few studies have focused on the mechanisms of pheromone perception. In this study, we identified the odorant receptor repertoire in three mirid bug species, Apolygus lucorum, Adelphocoris lineolatus, and Adelphocoris suturalis using antennal transcriptome sequencing and bioinformatics analysis. The candidate pheromone receptor (PR) genes were then identified by comparative transcriptomic and expression pattern analysis. Importantly, in vitro functional studies have shown that the candidate PRs have robust responses to the main mirid bug sex pheromone components (E)-2-hexenyl butyrate (E2HB) and hexyl butyrate (HB). Our study uncovered the mechanism of pheromone peripheral coding in these three species and elucidated the mechanism by which mirid bugs can specifically recognize a mate. Moreover, the results of our study will provide a theoretical basis for screening effective sex attractants or mating disturbance agents at the molecular and neural levels for enhanced control of these destructive pests.
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http://dx.doi.org/10.1016/j.ibmb.2021.103621DOI Listing
July 2021

The copper-catalyzed synthesis of dihydrooxazoles from α,β-unsaturated ketoximes and activated ketones.

Chem Commun (Camb) 2021 Jul 1;57(59):7272-7275. Epub 2021 Jul 1.

College of Biotechnology and Pharmaceutical Engineering, State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, 30 Puzhu Rd S, Nanjing, 211816, China.

The first copper-catalyzed [3+2]-type condensation reaction of α,β-unsaturated ketoximes with activated ketones has been described for the synthesis of dihydrooxazoles, especially trifluoromethyl-decorated dihydrooxazoles. Notable features of this method include its broad substrate scope, good functional group tolerance, and simple operation.
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http://dx.doi.org/10.1039/d1cc02422aDOI Listing
July 2021

Common and rare variant analyses combined with single-cell multiomics reveal cell-type-specific molecular mechanisms of COVID-19 severity.

medRxiv 2021 Jun 21. Epub 2021 Jun 21.

The determinants of severe COVID-19 in non-elderly adults are poorly understood, which limits opportunities for early intervention and treatment. Here we present novel machine learning frameworks for identifying common and rare disease-associated genetic variation, which outperform conventional approaches. By integrating single-cell multiomics profiling of human lungs to link genetic signals to cell-type-specific functions, we have discovered and validated over 1,000 risk genes underlying severe COVID-19 across 19 cell types. Identified risk genes are overexpressed in healthy lungs but relatively downregulated in severely diseased lungs. Genetic risk for severe COVID-19, within both common and rare variants, is particularly enriched in natural killer (NK) cells, which places these immune cells upstream in the pathogenesis of severe disease. Mendelian randomization indicates that failed NKG2D-mediated activation of NK cells leads to critical illness. Network analysis further links multiple pathways associated with NK cell activation, including type-I-interferon-mediated signalling, to severe COVID-19. Our rare variant model, PULSE, enables sensitive prediction of severe disease in non-elderly patients based on whole-exome sequencing; individualized predictions are accurate independent of age and sex, and are consistent across multiple populations and cohorts. Risk stratification based on exome sequencing has the potential to facilitate post-exposure prophylaxis in at-risk individuals, potentially based around augmentation of NK cell function. Overall, our study characterizes a comprehensive genetic landscape of COVID-19 severity and provides novel insights into the molecular mechanisms of severe disease, leading to new therapeutic targets and sensitive detection of at-risk individuals.
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http://dx.doi.org/10.1101/2021.06.15.21258703DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8240695PMC
June 2021

A fundamental viewpoint on the hydrogen spillover phenomenon of electrocatalytic hydrogen evolution.

Nat Commun 2021 Jun 9;12(1):3502. Epub 2021 Jun 9.

Key Laboratory of Special Functional and Smart Polymer Materials of Ministry of Industry and Information Technology, School of Chemistry and Chemical Engineering, Northwestern Polytechnical University, Xi'an, China.

Hydrogen spillover phenomenon of metal-supported electrocatalysts can significantly impact their activity in hydrogen evolution reaction (HER). However, design of active electrocatalysts faces grand challenges due to the insufficient understandings on how to overcome this thermodynamically and kinetically adverse process. Here we theoretically profile that the interfacial charge accumulation induces by the large work function difference between metal and support (∆Φ) and sequentially strong interfacial proton adsorption construct a high energy barrier for hydrogen transfer. Theoretical simulations and control experiments rationalize that small ∆Φ induces interfacial charge dilution and relocation, thereby weakening interfacial proton adsorption and enabling efficient hydrogen spillover for HER. Experimentally, a series of Pt alloys-CoP catalysts with tailorable ∆Φ show a strong ∆Φ-dependent HER activity, in which PtIr/CoP with the smallest ∆Φ = 0.02 eV delivers the best HER performance. These findings have conclusively identified ∆Φ as the criterion in guiding the design of hydrogen spillover-based binary HER electrocatalysts.
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http://dx.doi.org/10.1038/s41467-021-23750-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190308PMC
June 2021

Spatial intimacy of binary active-sites for selective sequential hydrogenation-condensation of nitriles into secondary imines.

Nat Commun 2021 Jun 7;12(1):3382. Epub 2021 Jun 7.

Key Laboratory of Special Functional and Smart Polymer Materials of Ministry of Industry and Information Technology, School of Chemistry and Chemical Engineering, Northwestern Polytechnical University, Xi'an, China.

Precisely controlling the spatial intimacy of multiple active sites at sub-nanoscale in heterogeneous catalysts can improve their selectivity and activity. Herein, we realize a highly selective nitrile-to-secondary imine transformation through a cascaded hydrogenation and condensation process by Pt/CoBO comprising the binary active sites of the single-dispersed Pt and interfacial Lewis acidic B. Atomic Pt sites with large inter-distances (>nanometers) only activate hydrogen for nitrile hydrogenation, but inhibit condensation. Both adjacent B…B on CoBO and neighbouring Pt…B pairs with close intimacy of ~0.45 nm can satisfy the spatial prerequisites for condensation. Mechanism investigations demonstrate the energetically favorable pathway occurred on adjacent Lewis acidic B sites through the nitrile adsorption (acid-base interaction), hydrogenation via hydrogen spillover from Pt to B sites and sequential condensation. Strong intermolecular tension and steric hindrance of secondary imines on active sites lead to their effective desorption and thereby a high chemoselectivity of secondary imines.
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http://dx.doi.org/10.1038/s41467-021-23705-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184996PMC
June 2021

Collagen/heparin sulfate scaffold combined with mesenchymal stem cells treatment for canines with spinal cord injury: A pilot feasibility study.

J Orthop Surg (Hong Kong) 2021 May-Aug;29(2):23094990211012293

Tianjin Key Laboratory of Neurotrauma Repair, Pingjin Hospital Brain Center, Characteristic Medical Center of Chinese People's Armed Police Force, Tianjin, China.

Background: Due to endogenous neuronal deficiency and glial scar formation, spinal cord injury (SCI) often leads to irreversible neurological loss. Accumulating evidence has shown that a suitable scaffold has important value for promoting nerve regeneration after SCI. Collagen/heparin sulfate scaffold (CHSS) has shown effect for guiding axonal regeneration and decreasing glial scar deposition after SCI. The current research aimed to evaluate the utility of the CHSSs adsorbed with mesenchymal stem cells (MSCs) on nerve regeneration, and functional recovery after acute complete SCI.

Methods: CHSSs were prepared, and evaluated for biocompatibility. The CHSSs adsorbed with MSCs were transplanted into these canines with complete SCI.

Results: We observed that MSCs had good biocompatibility with CHSSs. In complete transverse SCI models, the implantation of CHSS co-cultured with MSCs exhibited significant improvement in locomotion, motor evoked potential, magnetic resonance imaging, diffusion tensor imaging, and urodynamic parameters. Meanwhile, nerve fibers were markedly improved in the CHSS adsorbed with MSCs group. Moreover, we observed that the implantation of CHSS combined with MSCs modulated inflammatory cytokine levels.

Conclusions: The results preliminarily demonstrated that the transplantation of MSCs on a CHSS could improve the recovery of motor function after SCI. Thus, implanting the MSCs-laden CHSS is a promising combinatorial therapy for treatment in acute SCI.
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http://dx.doi.org/10.1177/23094990211012293DOI Listing
June 2021

Physical exercise is a risk factor for amyotrophic lateral sclerosis: Convergent evidence from Mendelian randomisation, transcriptomics and risk genotypes.

EBioMedicine 2021 Jun 26;68:103397. Epub 2021 May 26.

Sheffield Institute for Translational Neuroscience (SITraN), University of Sheffield, Sheffield, UK. Electronic address:

Background: Amyotrophic lateral sclerosis (ALS) is a universally fatal neurodegenerative disease. ALS is determined by gene-environment interactions and improved understanding of these interactions may lead to effective personalised medicine. The role of physical exercise in the development of ALS is currently controversial.

Methods: First, we dissected the exercise-ALS relationship in a series of two-sample Mendelian randomisation (MR) experiments. Next we tested for enrichment of ALS genetic risk within exercise-associated transcriptome changes. Finally, we applied a validated physical activity questionnaire in a small cohort of genetically selected ALS patients.

Findings: We present MR evidence supporting a causal relationship between genetic liability to frequent and strenuous leisure-time exercise and ALS using a liberal instrument (multiplicative random effects IVW, p=0.01). Transcriptomic analysis revealed that genes with altered expression in response to acute exercise are enriched with known ALS risk genes (permutation test, p=0.013) including C9ORF72, and with ALS-associated rare variants of uncertain significance. Questionnaire evidence revealed that age of onset is inversely proportional to historical physical activity for C9ORF72-ALS (Cox proportional hazards model, Wald test p=0.007, likelihood ratio test p=0.01, concordance=74%) but not for non-C9ORF72-ALS. Variability in average physical activity was lower in C9ORF72-ALS compared to both non-C9ORF72-ALS (F-test, p=0.002) and neurologically normal controls (F-test, p=0.049) which is consistent with a homogeneous effect of physical activity in all C9ORF72-ALS patients.

Interpretation: Our MR approach suggests a positive causal relationship between ALS and physical exercise. Exercise is likely to cause motor neuron injury only in patients with a risk-genotype. Consistent with this we have shown that ALS risk genes are activated in response to exercise. In particular, we propose that G4C2-repeat expansion of C9ORF72 predisposes to exercise-induced ALS.

Funding: We acknowledge support from the Wellcome Trust (JCK, 216596/Z/19/Z), NIHR (PJS, NF-SI-0617-10077; IS-BRC-1215-20017) and NIH (MPS, CEGS 5P50HG00773504, 1P50HL083800, 1R01HL101388, 1R01-HL122939, S10OD025212, P30DK116074, and UM1HG009442).
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http://dx.doi.org/10.1016/j.ebiom.2021.103397DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170114PMC
June 2021

Identification of VWF as a Novel Biomarker in Lung Adenocarcinoma by Comprehensive Analysis.

Front Oncol 2021 22;11:639600. Epub 2021 Apr 22.

NHC Key Laboratory of Cancer Proteomics, Department of Oncology, Xiangya Hospital, Central South University, Changsha, China.

Lung adenocarcinoma (LUAD) is one of the most malignant tumors with high morbidity and mortality worldwide due to the lack of reliable methods for early diagnosis and effective treatment. It's imperative to study the mechanism of its development and explore new biomarkers for early detection of LUAD. In this study, the Gene Expression Omnibus (GEO) dataset GSE43458 and The Cancer Genome Atlas (TCGA) were used to explore the differential co-expressed genes between LUAD and normal samples. Three hundred sixity-six co-expressed genes were identified by differential gene expression analysis and Weighted Gene Co-expression Network Analysis (WGCNA) method. Those genes were mainly enriched in ameboidal-type cell migration (biological process), collagen-containing extracellular matrix (cell component), and extracellular matrix structure constituent (molecular function). The protein-protein network (PPI) was constructed and 10 hub genes were identified, including IL6, VWF, CDH5, PECAM1, EDN1, BDNF, CAV1, SPP1, TEK, and SELE. The expression level of hub genes was validated in the GEPIA database, compared with normal tissues, VWF is lowly expressed and SPP1 is upregulated in LUAD tissues. The survival analysis showed increased expression of SPP1 indicated unfavorable prognosis whereas high expression of VWF suggested favorable prognosis in LUAD (p < 0.05). Based on the immune infiltration analysis, the relationship between SPP1 and VWF expression and macrophage, neutrophil, and dendritic cell infiltration was weak in LUAD. Quantitative real-time PCR (qRT-PCR) and western blotting were used to validate the expression of VWF and SPP1 in normal human bronchial epithelial (HBE) cell and three LUAD cell lines, H1299, H1975, and A549. Immunohistochemistry (IHC) was further performed to detect the expression of VWF in 10 cases LUAD samples and matched normal tissues. In summary, the data suggest that VWF is a potential novel biomarker for prognosis of LUAD.
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http://dx.doi.org/10.3389/fonc.2021.639600DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100660PMC
April 2021

Asymmetric Catalytic Approach to Multilayer 3D Chirality.

Chemistry 2021 May 1;27(30):7977. Epub 2021 May 1.

Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, Texas, 79409, USA.

Invited for the cover of this issue is Guigen Li and co-workers at Texas Tech University and Nanjing University. The cover artwork shows that chirality phenomena exists in the universe and in nature, including at micro and molecular levels. Read the full text of the article at 10.1002/chem.202100700.
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http://dx.doi.org/10.1002/chem.202101318DOI Listing
May 2021

Preparation and characterization of selenium-rich polysaccharide from Phellinus igniarius and its effects on wound healing.

Carbohydr Polym 2021 Jul 24;264:117982. Epub 2021 Mar 24.

Shandong Key Laboratory of Proteins and Peptides Pharmaceutical Engineering, Shandong Universities Key Laboratory of Biopharmaceuticals, School of Life Science and Technology, Weifang Medical University, Weifang, 261053, China. Electronic address:

The modified of polysaccharides show various bio-activities. In our work, Phellinus igniarius Selenium-enriched mycelias polysaccharides (PSeP) were prepared from Phellinus igniarius, and its antioxidant and anti-inflammatory effects on injured mice were evaluated. The selenium content and physical properties of polysaccharides were characterized by GC, HPGPC, and FT-IR analysis. The results showed that PSeP could reduce reactive oxygen species (ROS) levels, myeloperoxidase (MPO) activity as well as malondialdehyde (MDA) content. Meanwhile, it increased the enzyme activities of glutathione peroxidase (GSH-Px) and catalase (CAT). Finally, it showed obvious wound healing effects in vivo. Moreover, PSeP could clear the ROS without obvious cytotoxicity. PSeP could further improve its ability to clear ROS level to promote skin wound healing in mice three days in advance.
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http://dx.doi.org/10.1016/j.carbpol.2021.117982DOI Listing
July 2021

Asymmetric Catalytic Approach to Multilayer 3D Chirality.

Chemistry 2021 May 6;27(30):8013-8020. Epub 2021 May 6.

Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409, USA.

The first asymmetric catalytic approach to multilayer 3D chirality has been achieved by using Suzuki-Miyaura cross-couplings. New chiral catalysts were designed and screened under various catalytic systems that proved chiral amide-phosphines to be more efficient ligands than other candidates. The multilayer 3D framework was unambiguously determined by X-ray structural analysis showing a parallel pattern of three layers consisting of top, middle and bottom aromatic rings. The X-ray structure of a catalyst complex, dichloride complex of Pd-phosphine amide, was obtained revealing an interesting asymmetric environment nearby the Pd metal center. Three rings of multilayer 3D products can be readily changed by varying aromatic ring-anchored starting materials. The resulting multilayer products displayed strong luminescence under UV irradiation and strong aggregation-induced emission (AIE). In the future, this work would benefit not only the field of asymmetric synthesis but also materials science, in particular polarized organic electronics, optoelectronics and photovoltaics.
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http://dx.doi.org/10.1002/chem.202100700DOI Listing
May 2021

YALI0C22088g from Yarrowia lipolytica catalyses the conversion of l-methionine into volatile organic sulfur-containing compounds.

Microb Biotechnol 2021 Jul 1;14(4):1462-1471. Epub 2021 Apr 1.

Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Key Laboratory of Industrial Microbiology, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei University of Technology, Wuhan, 430068, China.

The enzymatic conversion of l-methionine (l-Met) into volatile organic sulfur-containing compounds (VOSCs) plays an important role in developing the characteristic aroma of foods. However, the mechanism for the direct conversion of l-Met into VOSCs is still unclear in yeast cells used to make food products. Here, we show that the transcription profile of YALI0C22088g from Yarrowia lipolytica correlates positively with l-Met addition. YALI0C22088g catalyses the γ-elimination of l-Met, directly converting l-Met into VOSCs. YALI0C22088g also exhibits strong C-S lysis activities towards l-cystathionine and the other sulfur-containing compounds and forms a distinct cystathionine-γ-lyase subgroup. We identified eight key amino acid residues in YALI0C22088g, and we inferred that the size of the tunnel and the charges carried by the entrance amino acid residue are the determinants for the enzymatic conversion of l-Met into VOSCs. These findings reveal the formation mechanism of VOSCs produced directly from l-Met via the demethiolation pathway in Yarrowia lipolytica, which provides a rationale for engineering the enzymatic conversion of l-Met into VOSCs and thus stimulates the enzymatic production of aroma compounds.
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http://dx.doi.org/10.1111/1751-7915.13796DOI Listing
July 2021

Precision medicine in women with epilepsy: The challenge, systematic review, and future direction.

Epilepsy Behav 2021 05 25;118:107928. Epub 2021 Mar 25.

Department of Department of Neurology & Neurological Sciences, Stanford University School of Medicine, Stanford, CA 94305, USA.

Epilepsy is one of the most prevalent neurologic conditions, affecting almost 70 million people worldwide. In the United States, 1.3 million women with epilepsy (WWE) are in their active reproductive years. Women with epilepsy (WWE) face gender-specific challenges such as pregnancy, seizure exacerbation with hormonal pattern fluctuations, contraception, fertility, and menopause. Precision medicine, which applies state-of-the art molecular profiling to diagnostic, prognostic, and therapeutic problems, has the potential to advance the care of WWE by precisely tailoring individualized management to each patient's needs. For example, antiseizure medications (ASMs) are among the most common teratogens prescribed to women of childbearing potential. Teratogens act in a dose-dependent manner on a susceptible genotype. However, the genotypes at risk for ASM-induced teratogenic deficits are unknown. Here we summarize current challenging issues for WWE, review the state-of-art tools for clinical precision medicine approaches, perform a systematic review of pharmacogenomic approaches in management for WWE, and discuss potential future directions in this field. We envision a future in which precision medicine enables a new practice style that puts focus on early detection, prediction, and targeted therapies for WWE.
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http://dx.doi.org/10.1016/j.yebeh.2021.107928DOI Listing
May 2021

The corticospinal tract structure of collagen/silk fibroin scaffold implants using 3D printing promotes functional recovery after complete spinal cord transection in rats.

J Mater Sci Mater Med 2021 Mar 22;32(4):31. Epub 2021 Mar 22.

Tianjin Key Laboratory of Neurotrauma Repair, Pingjin Hospital Brain Center, Characteristic Medical Center of PAPF, Tianjin, 300162, China.

No effective treatment has been established for nerve dysfunction caused by spinal cord injury (SCI). Orderly axonal growth at the site of spinal cord transection and creation of an appropriate biological microenvironment are important for functional recovery. To axially guiding axonal growth, designing a collagen/silk fibroin scaffold fabricated with 3D printing technology (3D-C/SF) emulated the corticospinal tract. The normal collagen/silk fibroin scaffold with freeze-drying technology (C/SF) or 3D-C/SF scaffold were implanted into rats with completely transected SCI to evaluate its effect on nerve repair during an 8-week observation period. Electrophysiological analysis and locomotor performance showed that the 3D-C/SF implants contributed to significant improvements in the neurogolical function of rats compared to C/SF group. By magnetic resonance imaging, 3D-C/SF implants promoted a striking degree of axonal regeneration and connection between the proximal and distal SCI sites. Compared with C/SF group, rats with 3D-C/SF scaffold exhibited fewer lesions and disordered structures in histological analysis and more GAP43-positive profiles at the lesion site. The above results indicated that the corticospinal tract structure of 3D printing collagen/silk fibroin scaffold improved axonal regeneration and promoted orderly connections within the neural network, which could provided a promising and innovative approach for tissue repair after SCI.
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http://dx.doi.org/10.1007/s10856-021-06500-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7985105PMC
March 2021

Characterizing RNA Pseudouridylation by Convolutional Neural Networks.

Genomics Proteomics Bioinformatics 2021 Feb 22. Epub 2021 Feb 22.

Institute for Interdisciplinary Information Sciences, Tsinghua University, Beijing 100084, China; MOE Key Laboratory of Bioinformatics, Tsinghua University, Beijing 100084, China. Electronic address:

Pseudouridine (Ψ) is the most prevalent post-transcriptional RNA modification and is widespread in small cellular RNAs and mRNAs. However, the functions, mechanisms and precise distribution of Ψs (especially in mRNAs) still remain largely unclear. The landscape of Ψs across the transcriptome has not yet been fully delineated. Here, we present a highly effective model based on a convolutional neural network (CNN), called PULSE, to analyze large-scale profiling data of Ψ sites and characterize the contextual sequence features of pseudouridylation. PULSE, consisting of two alternatively stacked convolution and pooling layers followed by a fully-connected neural network, can automatically learn the hidden patterns of pseudouridylation from the local sequence information. Extensive validation tests demonstrated that PULSE can outperform other state-of-the-art prediction methods and achieve high prediction accuracy, thus enabling us to further characterize the transcriptome-wide landscape of Ψ sites. We further showed that the prediction results derived from PULSE can provide novel insights into understanding the functional roles of pseudouridylation, such as the regulations of RNA secondary structure, codon usage, translation, and RNA stability, and the connection to single nucleotide variants.
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http://dx.doi.org/10.1016/j.gpb.2019.11.015DOI Listing
February 2021

Triple-Columned and Multiple-Layered 3D Polymers: Design, Synthesis, Aggregation-Induced Emission (AIE), and Computational Study.

Research (Wash D C) 2021 8;2021:3565791. Epub 2021 Feb 8.

Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, Texas 79409-1061, USA.

Conjugated polymers and oligomers have great potentials in various fields, especially in materials and biological sciences because of their intriguing electronic and optoelectronic properties. In recent years, the through-space conjugation system has emerged as a new assembled pattern of multidimensional polymers. Here, a novel series of structurally condensed multicolumn/multilayer 3D polymers and oligomers have been designed and synthesized through one-pot Suzuki polycondensation (SPC). The intramolecularly stacked arrangement of polymers can be supported by either X-ray structural analysis or computational analysis. In all cases, polymers were obtained with modest to good yields, as determined by GPC and H-NMR. MALDI-TOF analysis has proven the speculation of the step-growth process of this polymerization. The computational study of ab initio and DFT calculations based on trimer and pentamer models gives details of the structures and the electronic transition. Experimental results of optical and AIE research confirmed by calculation indicates that the present work would facilitate the research and applications in materials.
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http://dx.doi.org/10.34133/2021/3565791DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7888304PMC
February 2021

Effects of salicylic acid, zinc and glycine betaine on morpho-physiological growth and yield of maize under drought stress.

Sci Rep 2021 Feb 4;11(1):3195. Epub 2021 Feb 4.

College of Agronomy and Biotechnology, Southwest University, Chongqing, 400715, China.

Drought is one of the major environmental stresses that negatively affect the maize (Zea mays L.) growth and production throughout the world. Foliar applications of plant growth regulators, micronutrients or osmoprotectants for stimulating drought-tolerance in plants have been intensively reported. A controlled pot experiment was conducted to study the relative efficacy of salicylic acid (SA), zinc (Zn), and glycine betaine (GB) foliar applications on morphology, chlorophyll contents, relative water content (RWC), gas-exchange attributes, activities of antioxidant enzymes, accumulations of reactive oxygen species (ROS) and osmolytes, and yield attributes of maize plants exposed to two soil water conditions (85% field capacity: well-watered, 50% field capacity: drought stress) during critical growth stages. Drought stress significantly reduced the morphological parameters, yield and its components, RWC, chlorophyll contents, and gas-exchange parameters except for intercellular CO concentration, compared with well water conditions. However, the foliar applications considerably enhanced all the above parameters under drought. Drought stress significantly (p < 0.05) increased the hydrogen peroxide and superoxide anion contents, and enhanced the lipid peroxidation rate measured in terms of malonaldehyde (MDA) content. However, ROS and MDA contents were substantially decreased by foliar applications under drought stress. Antioxidant enzymes activity, proline content, and the soluble sugar were increased by foliar treatments under both well-watered and drought-stressed conditions. Overall, the application of GB was the most effective among all compounds to enhance the drought tolerance in maize through reduced levels of ROS, increased activities of antioxidant enzymes and higher accumulation of osmolytes contents.
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http://dx.doi.org/10.1038/s41598-021-82264-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7862227PMC
February 2021

Bloodletting Puncture at Hand Twelve Jing-Well Points Relieves Brain Edema after Severe Traumatic Brain Injury in Rats via Inhibiting MAPK Signaling Pathway.

Chin J Integr Med 2021 Apr 30;27(4):291-299. Epub 2021 Jan 30.

Research Center of Experimental Acupuncture Science, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China.

Objective: To investigate whether blood-brain barrier (BBB) served a key role in the edema-relief effect of bloodletting puncture at hand twelve Jing-well points (HTWP) in traumatic brain injury (TBI) and the potential molecular signaling pathways.

Methods: Adult male Sprague-Dawley rats were assigned to the sham-operated (sham), TBI, and bloodletting puncture (bloodletting) groups (n=24 per group) using a randomized number table. The TBI model rats were induced by cortical contusion and then bloodletting puncture were performed at HTWP twice a day for 2 days. The neurological function and cerebral edema were evaluated by modified neurological severity score (mNSS), cerebral water content, magnetic resonance imaging and hematoxylin and eosin staining. Cerebral blood flow was measured by laser speckles. The protein levels of aquaporin 4 (AQP4), matrix metalloproteinases 9 (MMP9) and mitogen-activated protein kinase pathway (MAPK) signaling were detected by immunofluorescence staining and Western blot.

Results: Compared with TBI group, bloodletting puncture improved neurological function at 24 and 48 h, alleviated cerebral edema at 48 h, and reduced the permeability of BBB induced by TBI (all P<0.05). The AQP4 and MMP9 which would disrupt the integrity of BBB were downregulated by bloodletting puncture (P<0.05 or P<0.01). In addition, the extracellular signal-regulated kinase (ERK) and p38 signaling pathways were inhibited by bloodletting puncture (P<0.05).

Conclusions: Bloodletting puncture at HTWP might play a significant role in protecting BBB through regulating the expressions of MMP9 and AQP4 as well as corresponding regulatory upstream ERK and p38 signaling pathways. Therefore, bloodletting puncture at HTWP may be a promising therapeutic strategy for TBI-induced cerebral edema.
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http://dx.doi.org/10.1007/s11655-021-3326-5DOI Listing
April 2021

[Corrigendum] Ouabain suppresses the growth and migration abilities of glioma U‑87MG cells through inhibiting the Akt/mTOR signaling pathway and downregulating the expression of HIF‑1α.

Mol Med Rep 2021 03 26;23(3). Epub 2021 Jan 26.

Institute of TBI and Neuroscience of Chinese People's Armed Police Force, Tianjin Key Laboratory of Neurotrauma Repair, Center for Neurology and Neurosurgery of Affiliated Hospital of Logistics University of PAP, Tianjin 300162, P.R. China.

Subsequently to the publication of the above paper, an interested reader drew to the authors' attention that several pairings of panels in Fig. 5, as shown on p. 5599, were strikingly similar. After having examined their original data, the authors realized that they uploaded some images incorrectly during the process of compiling this figure, and that there were duplicated data panels in this figure. However, the authors were able to consult their original data, and had access to the correct images. The revised version of Fig. 5, showing the correct data for the Akt/Control, p‑Akt/Control, mTOR/0.05 μM Ouabain, HIF‑1α/0.05 μM Ouabain and Akt/0.5 μM Ouabain experiments, is shown opposite. Note that the replacement of the erroneous data does not affect either the results or the conclusions reported in this paper, and all the authors agree to this Corrigendum. The authors are grateful to the Editor of Molecular Medicine Reports for granting them this opportunity to publish a Corrigendum, and apologize to the readership for any inconvenience caused. [the original article was published in Molecular Medicine Reports 17: 5595‑5600, 2018; DOI: 10.3892/mmr.2018.8587].
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http://dx.doi.org/10.3892/mmr.2021.11836DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7821337PMC
March 2021

Implantation of regenerative complexes in traumatic brain injury canine models enhances the reconstruction of neural networks and motor function recovery.

Theranostics 2021 1;11(2):768-788. Epub 2021 Jan 1.

Tianjin Key Laboratory of Neurotrauma Repair, Institute of Traumatic Brain Injury and Neuroscience, Center for Neurology and Neurosurgery of Characteristic Medical Center of Chinese People's Armed Police Force (PAP), Chenglin Road No.220, Tianjin 300162, China.

The combination of medical and tissue engineering in neural regeneration studies is a promising field. Collagen, silk fibroin and seed cells are suitable options and have been widely used in the repair of spinal cord injury. In this study, we aimed to determine whether the implantation of a complex fabricated with collagen/silk fibroin (SF) and the human umbilical cord mesenchymal stem cells (hUCMSCs) can promote cerebral cortex repair and motor functional recovery in a canine model of traumatic brain injury (TBI). A porous scaffold was fabricated with cross-linked collagen and SF. Its physical properties and degeneration rate were measured. The scaffolds were co-cultured with hUCMSCs after which an implantable complex was formed. After complex implantation to a canine model of TBI, the motor evoked potential (MEP) and magnetic resonance imaging (MRI) were used to evaluate the integrity of the cerebral cortex. The neurologic score, motion capture, surface electromyography (sEMG), and vertical ground reaction force (vGRF) were measured in the analysis of motor functions. In vitro analysis of inflammation levels was performed by Elisa while immunohistochemistry was used in track the fate of hUCMSCs. In situ hybridization, transmission electron microscope, and immunofluorescence were used to assess neural and vascular regeneration. Favorable physical properties, suitable degradation rate, and biocompatibility were observed in the collagen/SF scaffolds. The group with complex implantation exhibited the best cerebral cortex integrity and motor functions. The implantation also led to the regeneration of more blood vessels and nerve fibers, less glial fibers, and inflammatory factors. Implantation of this complex enhanced therapy in traumatic brain injury (TBI) through structural repair and functional recovery. These effects exhibit the translational prospects for the clinical application of this complex.
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http://dx.doi.org/10.7150/thno.50540DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7738861PMC
January 2021

The complete plastid genome of (Orchidaceae, Vandeae).

Mitochondrial DNA B Resour 2020 Jan 21;5(1):390-391. Epub 2020 Jan 21.

Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou, PR China.

The complete plastid genome of was determined and analyzed in this work. The plastome was 147,715 bp in length with 84,094 bp of the large single-copy (LSC) region, 12,073 bp of the small single-copy (SSC) region and 25,774 bp of the inverted repeat (IRs) regions. The genome contained 120 genes, 74 protein-coding genes, 38 tRNA genes, and 8 rRNA genes. Phylogenetic analysis of 20 Aeridinae plastomes suggested three groups of were divided, and was sister to .
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http://dx.doi.org/10.1080/23802359.2019.1704185DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7748776PMC
January 2020

The complete plastid genome of (Orchidaceae, Aeridinae).

Mitochondrial DNA B Resour 2020 Jan 21;5(1):384-385. Epub 2020 Jan 21.

Fujian Colleges and Universities Engineering Research Institute of Conservation and Utilization of Natural Bioresources, College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, China.

The complete plastid genome of was determined and analyzed in this work. The plastome was 149,689 bp in length with 86,778 bp of the large single-copy (LSC) region, 12,129 bp of the small single-copy (SSC) region and 25,391 bp of the inverted repeat (IR) regions. The genome contained 120 genes, 74 protein-coding genes, 38 tRNA genes, and 8 rRNA genes. Phylogenetic analysis of 17 Aeridinae plastomes suggested four groups were divided, and was sister to .
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http://dx.doi.org/10.1080/23802359.2019.1703594DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7748503PMC
January 2020

Complete plastid genome of (Orchidaceae, Goodyerinae).

Mitochondrial DNA B Resour 2019 Dec 11;5(1):127-128. Epub 2019 Dec 11.

Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou, China.

The first complete plastid genome of , , was assembled and analyzed in this study. The total genome was 147,218 bp in length, consisting of a large single-copy region (LSC) of 81,081 bp, a small single-copy region (SSC) of 14,769 bp, and two inverted repeat regions (IRA and IRB) of 25,684 bp. The genome contained 131 genes, including 38 transfer RNA (tRNA) genes, 8 ribosomal RNA (rRNA) genes and 85 protein-coding genes. Phylogenomic analysis indicated that nested within Goodyerinae.
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http://dx.doi.org/10.1080/23802359.2019.1698347DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7720991PMC
December 2019

The complete plastid genome of (Orchidaceae, Aeridinae).

Mitochondrial DNA B Resour 2019 Nov 12;4(2):3985-3986. Epub 2019 Nov 12.

Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou, China.

The complete plastid genome of was determined and analyzed in this work. The plastome was 146,681 bp in length with 83,920 bp of the large single-copy (LSC) region, 11,751 bp of the small single-copy (SSC) region and 25,505 bp of the inverted repeat (IR) regions. The genome contained 120 genes, 74 protein-coding genes, 38 tRNA genes, and 8 rRNA genes. Phylogenetic analysis suggested is sister to plus .
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http://dx.doi.org/10.1080/23802359.2019.1688702DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7707680PMC
November 2019

Overexpression of LVRN impedes the invasion of trophoblasts by inhibiting epithelial-mesenchymal transition.

Acta Biochim Biophys Sin (Shanghai) 2021 Feb;53(2):249-257

Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China.

Laeverin (LVRN) was first detected on the outer layer of the chorion laeve and migrating extravillous trophoblasts (EVTs). It is an enzyme that plays an important role in the placentation and pathophysiology of preeclampsia (PE). Previous studies have indicated that LVRN may be required for the invasion of human trophoblast cells. Paradoxically, LVRN was found to be highly expressed in the trophoblasts of PE patients with impaired invasive capacities. In this study, we detected the expression of LVRN in the placentas of PE patients (n=5) and normal term pregnancy women (n=5) as a control group by immunohistochemistry. LVRN was elevated in decidua (P=0.0083) and villi (P=0.0079) of PE patients. Next, LVRN was overexpressed via adeno-associated virus-mediated gene transfer in trophoblastic cell lines HTR8, Swan71, and JAR. Matrigel transwell assay and wound healing assay showed that overexpression of LVRN impeded the invasion of these three cell lines. Western blot analysis showed that LVRN overexpression caused downregulation of N-cadherin and vimentin and upregulation of E-cadherin, suggesting the inhibitory role of LVRN in epithelial-mesenchymal transition (EMT). Moreover, our data indicated that long noncoding RNA NONSTAT103348 (lnc10-7) was elevated in PE patients. Silencing lnc10-7 led to decreased LVRN expression. Taken together, although the basal level of LVRN may be crucial for cell invasion, overexpression of LVRN may abrogate the cell invasiveness, suggesting a multifaceted role of LVRN in the pathogenesis of PE.
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http://dx.doi.org/10.1093/abbs/gmaa167DOI Listing
February 2021
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