Publications by authors named "Saeed Mohammadi"

142 Publications

MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF THE PLAGUE VECTOR XENOPSYLLA BRASILIENSIS.

J Parasitol 2021 Mar;107(2):289-294

Centre for Ecological Genomics and Wildlife Conservation, Department of Zoology, University of Johannesburg, Johannesburg, South Africa.

Members of the flea family Pulicidae have been the focus of many studies due to their significance as diseases vectors of medical and veterinary importance and their cosmopolitan distribution. They often exhibit variation in morphological features that can make correct species identification and management challenging. This may also apply to Xenopsylla brasiliensis (Baker, 1904), an important plague vector. In the current study, we aimed to provide genetic tools for reliable species identification using a DNA barcoding approach. A total of 73 flea specimens was collected from a native host (Namaqua rock mouse, Micaelamys namaquensis) in South Africa and identified morphologically. In addition, we took measurements of 7 morphological characteristics. Subsequently, we successfully generated barcodes of the mitochondrial cytochrome c oxidase subunit I (COI) gene for X. brasiliensis. We validated this approach by comparing our data to COI sequences from Rwandan X. brasiliensis. While sequences from both regions suggested a close relationship between the 2 X. brasiliensis populations, both haplotype and nucleotide diversity were substantially larger for the South African specimens. This may be attributed to human-assisted spread, differences in habitat, and/or host species sampled and merits further study in the future.
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http://dx.doi.org/10.1645/20-44DOI Listing
March 2021

Molecular mechanisms of vitamin D plus Bisphenol A effects on adipogenesis in human adipose-derived mesenchymal stem cells.

Diabetol Metab Syndr 2021 Apr 9;13(1):41. Epub 2021 Apr 9.

Department of Biostatistics, School of Public Health, Iran University of Medical Sciences, Tehran, Iran.

Background: Obesity is considered a major health concern and mounting evidence suggests that the exposure to environmental endocrine disruptors, including Bisphenol-A (BPA), may enhance the risk to develop the disease. Moreover, growing documents propose that the vitamin D may contribute to adipogenic signaling and lipid accumulation during adipocyte differentiation. We focused on the molecular mechanism of vitamin D and BPA in human adipose-derived mesenchymal stem cells (hADMSCs) which vitamin D and BPA may influence adipose tissue development and function.

Methods: Human adipose-derived mesenchymal stem cells were cultured for 14 days in lipogenic differentiation media containing continuous concentrations of vitamin D plus BPA (0.1 nM or 10 nM). The expression of adipogenic markers including the peroxisome proliferator-activated receptor γ (PPARγ), CCAAT-enhancer-binding protein α (C/EBP α) CCAAT-enhancer-binding protein β (C/EBP β), fatty acid synthase (FASN), lipoprotein lipase (LPL), sterol regulatory element-binding protein-1c (SREBP1c), insulin-induced gene-2 (INSIG2), vitamin D receptor (VDR), estrogen receptor-beta (ER-β), fatty acid-binding protein-4 (FABP4), and glucose transporter-4 (GLUT4) was measured using Quantitative polymerase chain reaction (qPCR) and enzyme-linked immunosorbent assay (ELISA). Lipid accumulation was visualized with staining with Oil Red O.

Results: In the morphological assessment of mesenchymal stem cells treated with a concentration of 10 nM vitamin D plus BPA, more lipid accumulations were observed in comparison with the group with 0.1 nM concentration. Treatment of hADMSCs with vitamin D plus BPA (0.1 nM) significantly inhibited the induction of PPARγ, C/EBP β, C/EBP α, and FASN related to adipocyte differentiation and development. However, the exposure of cells to the concentration of 10 nM vitamin D plus BPA induced the expression of these genes associated to the adipogenesis. The remarkable increase in the level of SREBP1c was associated to the suppression of INSIG2 in treated preadipocytes with 10 nM vitamin D plus BPA. Our findings showed that the expression of VDR, ERβ, GLUT4, and FABP4 were upregulated through differentiation with the highest concentrations in 0.1 nM vitamin D plus BPA group for VDR, ERβ, and GLUT4.

Conclusions: Vitamin D plus BPA at concentration of 10 nM boosted the adipogenesis during the critical stages of adipocytes development, whereas it seems to inhibit this process at concentration of 0.1 nM.
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http://dx.doi.org/10.1186/s13098-021-00661-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8033712PMC
April 2021

1,25-Dihydroxyvitamin D3 modulates adipogenesis of human adipose-derived mesenchymal stem cells dose-dependently.

Nutr Metab (Lond) 2021 Mar 12;18(1):29. Epub 2021 Mar 12.

Department of Biostatics, School of Public Health, Iran University of Medical Sciences, Tehran, Iran.

Purpose: 1,25-dihydroxyvitamin D3 may regulate adipogenesis in adipocytes in-vitro, but little is known about possible molecular mechanisms related to the inhibitory effect of 1,25-dihydroxyvitamin D3 on adipogenesis in humans҆ adipose tissue.

Methodology: In this study, human adipose-derived mesenchymal stem cells (hASCs) were cultured for 14 days in adipogenic differentiation media containing concentrations of 1,25-dihydroxyvitamin D3 (10-10 M). The extent of adipogenic differentiation in ASCs was assessed by Oil Red O staining and quantitative polymerase chain reaction (PCR) to determine expression levels of key adipogenic markers.

Results: Our results showed that vitamin D receptor (VDR), as a mediator of most actions of 1,25-dihydroxyvitamin D3, glucose trasporter-4 (GLUT4),and fatty acid binding protein-4 (FABP4) was expressed in vitamin D-treated hASCs. However, the protein level of these markers was lower than the control group. Treatment of human preadipocytes with 1,25-dihydroxyvitamin D3 significantly altered expression of adipogenic markers and triglyceride accumulation in a dose-dependent manner. 1,25-dihydroxyvitamin D3 at concentration of 10 M enhanced expression of sterol regulatory element-binding protein-1c (SREBP1c), CCAAT-enhancer-binding protein-β (C/EBPβ), a mitotic clonal expansion, peroxisome proliferator-activated receptor-gamma (PPARγ), fatty acid synthase (FASN), a marker of de novo lipogenesis,and lipoprotein lipase (LPL).

Conclusion: Our findings revealed that 1,25-dihydroxyvitamin D3 may provoke adipocyte development in critical periods of adipogenesis at concentration of 10 M, thereby leading to a greater risk of obesity in adulthood and an augmented risk of obesity-related diseases including diabetes, cardiovascular diseases, and some cancers.
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http://dx.doi.org/10.1186/s12986-021-00561-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7953614PMC
March 2021

Bupleurum falcatum L. alleviates nociceptive and neuropathic pain: Potential mechanisms of action.

J Ethnopharmacol 2021 Jun 6;273:113990. Epub 2021 Mar 6.

Department of Physiology, School of Medicine, Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.

Ethnopharmacological Relevance: In Iranian folkloric medicine, Bupleurum falcatum L. (Chinese Thoroughwax) has been used as a selective analgesic remedy for several centuries.

Objective: The current research was conducted to explore the anti-nociceptive and anti-allodynic action of Bupleurum falcatum L. roots essential oil (BFEO) in Swiss mice.

Materials And Methods: Formalin-induced paw licking (FIPL) model was applied for exploring of BFEO antinociceptive effects (neurogenic or inflammatory pain). The involvements of L-arginine-NO-cGMP-KATP channel pathway and several receptors such as opioid, peroxisome proliferator-activated (PPA), cannabinoid, transient receptor potential vanilloid, and adrenergic receptors were assesses to detect the anti-nociceptive activity of BFEO. Cervical spinal cord contusion (CSC) paradigm was employed for induction of neuropathic pain.

Results: BFEO (100 mg/kg), in the FIPL model, produced significant antinociception compared to the control mice (p < 0.01). Furthermore, L-arginine, methylene blue, glibenclamide, naloxonazine, GW9662, and SR141716A pre-treatments restored the BFEO anti-nociceptive effects (p < 0.05) in the FIPL (second phase) test (p < 0.05). Intraperitoneal administration of saikosaponin A (one of the main constituents of BFEO) partially alleviated (p < 0.05) pain in FIPL test. Likewise, in CSC mice, the von Frey assay exhibited that BFEO could alter mechanical allodynia.

Conclusion: Finally, it seems that, in male mice, BFEO has both anti-allodynic and anti-nociceptive effects. The present data also suggest activating the L-arginine-NO-cGMP-KATP channel pathway as well as interaction of opioid, PPA, and cannabinoid receptors in the BFEO anti-nociceptive activities. These results also propose that BFEO could effectively attenuate allodynia in CSC mice.
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http://dx.doi.org/10.1016/j.jep.2021.113990DOI Listing
June 2021

The migration of metastatic breast cancer cells is regulated by matrix stiffness via YAP signalling.

Heliyon 2021 Feb 13;7(2):e06252. Epub 2021 Feb 13.

Department of Mechanical Engineering, McMaster University, Hamilton, ON L8S 0A3, Canada.

Matrix stiffness is a driver of breast cancer progression and mechanosensitive transcriptional activator YAP plays an important role in this process. However, the interplay between breast cancer and matrix stiffness, and the significance of this interplay remained largely unknown. Here, we showed an increase in YAP nuclear localization and a higher proliferation rate in both highly metastatic MDA-MB-231 cells and the non-metastatic counterpart MCF-7 cells when they were exposed to the stiff matrix. However, in response to the stiff matrix highly metastatic MDA-MB-231 cells instead of MCF-7 cells exhibited upregulated mobility, which was shown to be YAP-dependent. Consistently, MDA-MB-231 cells exhibited different focal adhesion dynamics from MCF-7 cells in response to matrix stiffness. These results suggested a YAP-dependent mechanism through which matrix stiffness regulates the migratory potential of metastatic breast cancer cells.
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http://dx.doi.org/10.1016/j.heliyon.2021.e06252DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7895759PMC
February 2021

Interpretation of Hematological, Biochemical, and Immunological Findings of COVID-19 Disease: Biomarkers Associated with Severity and Mortality.

Iran J Allergy Asthma Immunol 2021 Feb 11;20(1):46-66. Epub 2021 Feb 11.

Department of Biostatistics and Social Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.

The severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) spread rapidly all over the world in late 2019 and caused critical illness and death in some infected patients. This study aimed at examining several laboratory factors, especially inflammatory and immunological mediators, to identify severity and mortality associated biomarkers. Ninety-three hospitalized patients with confirmed coronavirus disease 2019 (COVID-19) were classified based on disease severity. The levels of biochemical, hematological, immunological, and inflammatory mediators were assessed, and their association with severity and mortality were evaluated. Hospitalized patients were mostly men (77.4%) with an average (standard deviation) age of 59.14 (14.81) years. The mortality rate was significantly higher in critical patients (85.7%). Increased serum levels of blood sugar, urea, creatinine, uric acid, phosphorus, total bilirubin, serum glutamic-oxaloacetic transaminase, serum glutamic-oxaloacetic transaminase, lactic dehydrogenase, C-reactive protein, ferritin, and procalcitonin were significantly prevalent (p=0.002, p<0.001, p<0.001, p=0.014, p=0.047, p=0.003, p<0.001, p<0.001, p<0.001, p<0.001, P<0.001, and p<0.001, respectively) in COVID-19 patients. Decreased red blood cell, hemoglobin, and hematocrit were significantly prevalent among COVID-19 patients than healthy control subjects (p<0.001 for all). Troponin-I, interleukin-6, neutrophil/lymphocyte ratio (NLR), procalcitonin, and D-dimer showed a significant association with the mortality of patients with specificity and sensitivity more than 60%. Age, sex, underlying diseases, blood oxygen pressure, complete blood count along with C-reactive protein, lactic dehydrogenase, procalcitonin, D-dimer, and interleukin-6 evaluation help to predict the severity and required management for COVID-19 patients. Further investigations are highly recommended in a larger cohort study for validation of the present findings.
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http://dx.doi.org/10.18502/ijaai.v20i1.5412DOI Listing
February 2021

Antinociceptive activity of . leaf extract: a mechanistic evaluation.

Res Pharm Sci 2020 Oct 19;15(5):463-472. Epub 2020 Oct 19.

Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, I.R. Iran.

Background And Purpose: , a medicinal herb, traditionally had been used for the treatment of stomachache pain. In this study, the possible efficacy of leaf methanolic extract (CBHE) and also cnicin, one of its major constituents, was measured on pain.

Experimental Approach: In this study, pain assessment tests include writhing, tail-flick (TF), and formalin- induced paw licking test (FIPLT) were used. To understand the possible mediated anti-nociceptive mechanism of CBHE, the opioid mechanism(s), and involvement of the L-arginine/ nitric oxide/cGMP/ATP-sensitive potassium channel pathway (LNCaP) were scrutinized.

Findings/results: In TF and writhing tests, CBHE (150 and 300 mg/kg, i.p) remarkably exhibited an anti-nociceptive effect compared to that of the control. Furthermore, CBHE (150 and 300 mg/kg, i.p) in comparison with the control showed a noteworthy anti-nociceptive effect ( < 0.01) in the tonic phase of FIPLT. In the writhing test, administration of selective opioid antagonist (naltrindole, nor-binaltorphimine, and naloxonazine) attenuated the anti-nociceptive effect of CBHE (300 mg/kg) in comparison with control. Moreover, pre-treatment with Nω-nitro-L-arginine methyl ester hydrochloride, L-arginine hydrochloride, and glibenclamide significantly blocked the CBHE (300 mg/kg) anti-nociception ( < 0.05) while administration of sodium nitroprusside remarkably potentiated ( < 0.05) the antinociception induced by CBHE in the tonic phase of the FIPLT. Besides, cnicin (30 mg/kg) showed noteworthy anti-nociceptive effects in writhing, TF, and FIPLT paradigms.

Conclusion And Implications: Taken together, we elucidate that both CBHE and cnicin demonstrated antinociceptive effects in behavioral tests. The possible mechanisms of CBHE antinociception may involve in various neural signaling and modulatory pathways including LNCaP and opioidergic mechanisms.
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http://dx.doi.org/10.4103/1735-5362.297849DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7879793PMC
October 2020

Giant Cell Tumor: Changing Behavior from Intraorbital to Intraosseous Mass.

J Curr Ophthalmol 2020 Oct-Dec;32(4):414-416. Epub 2020 Dec 12.

Eye Research Center, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran.

Purpose: To present a patient with giant cell tumor (GCT) of the orbit by changing behavior from an intraorbital mass to an intraosseous tumor.

Methods: A 16-year-old boy presented with pain, swelling, erythematous of the left upper and lower eyelids, proptosis, and diplopia. Ophthalmic examination revealed chemosis, conjunctival injection, limited elevation, depression as well as abduction in the left eye.

Results: Multislice computed tomography scan (CT scan) of the orbit and paranasal sinuses showed a hyperdense, oval, extraconal mass with bone erosion. Magnetic resonance imaging of the orbit showed an inferior lateral isointense, oval, extraconal mass that had indented the globe. The patient underwent superior lateral orbitotomy, and the orbital mass was excised. Two months later, the patient developed proptosis, severe chemosis, and eyelid erythema in the same eye. CT scan showed an intraosseous mass in the lateral wall of the orbit that had pushed the globe anteromedially. Intraosseous tumor was resected, and the lateral orbital wall was drilled during the second surgery. GCT was diagnosed based on pathological survey.

Conclusion: Following the resection of the orbital GCT, the tumor behavior may change to an intraosseous lesion.
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http://dx.doi.org/10.4103/JOCO.JOCO_63_20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7861103PMC
December 2020

Infectious keratitis: trends in microbiological and antibiotic sensitivity patterns.

Eye (Lond) 2021 Jan 19. Epub 2021 Jan 19.

Ophthalmology Department and Eye Research Center, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran.

Objectives: To report the spectrum and trends of isolated microorganisms and their antibiotic susceptibility profile in patients with infectious keratitis in a 6-year period at a referral centre in Tehran.

Methods: The microbiology records of all corneal scrapings with a diagnosis of infectious keratitis were reviewed.

Results: A total of 6282 corneal scrapings were performed during the study period, of which 2479 (39.5%) samples were culture positive. Pseudomonas aeruginosa was found to be the most common causative agent in patients with keratitis, although Streptococcus pneumonia was the most prevalently isolated microorganism in patients older than 50 years. Fusarium sp. was the most common responsible pathogen in patients with fungal keratitis. The prevalence of bacterial keratitis due to gram positive microorganisms increased over time, however the number of Pseudomonas keratitis decreased in the second half of the study. Gram negative organisms showed a good sensitivity to levofloxacin, however, 34.1% of S. aureus isolates and 29.7% of coagulase negative staphylococci were resistant to this antibiotic. The odds of ciprofloxacin and levofloxacin resistance increased 1.25 and 1.15 for each 1-year increase in culture date, respectively (P < 0.001, P = 0.004).

Conclusions: We documented an increasing trend in the percentage of gram positive bacteria. Levofloxacin monotherapy might still be a good option in patients with gram negative bacterial keratitis, however owing to increasing resistance of staphylococci to fluoroquinolones, a regimen consisting of a combination of fortified antibiotics may be more effective in staphylococcal keratitis.
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http://dx.doi.org/10.1038/s41433-020-01378-wDOI Listing
January 2021

Caring-Related Chronic Low Back Pain and Associated Factors among Mothers of Children with Cerebral Palsy.

Rehabil Res Pract 2020 30;2020:8854435. Epub 2020 Dec 30.

Student Research Committee, School of Public Health, Iran University of Medical Sciences, Tehran, Iran.

Background: Literature indicated some risk factors for low back pain; however, there is insufficient knowledge on the effect of caring-related physical activities and individual characteristics on Chronic Low Back Pain (CLBP) in mothers of children with Cerebral Palsy (CP).

Objective: The main aim of the current study was to determine the association between caring-related physical activities, Body Mass Index (BMI), education level, and CLBP in mothers of children with CP.

Design: Case-control observational study. . Pediatric rehabilitation clinics. . Mothers of children with CP. . Measures is comprised of a self-administered questionnaire that included the demographic characteristics items, pain visual analog scale, and three items of the job-related physical demands questionnaire. The logistic regression model served to assess the association.

Results: The control group included 81 healthy mothers, with a mean (SD) age of 39 (8.45) years, and the case group contained 90 mothers who suffered from CLBP, with a mean (SD) age of 37 (8.64) years. Performing lifting movements (OR 13.73, = 2.62, < .001), BMI (OR 11.85, = 2.47, = .011), repetitive bending (OR 7.67, = 2.04, = .010), forward-flexion (OR 6.71, = 1.91, = .033), and level of education (OR .21, = -1.53, = .020), in descending order of odds ratios, were found to be significant predictors of the CLBP in mothers of children with CP.

Conclusion: Avoiding caring-related harmful physical activities, maintaining body weight within a healthy range, and increasing knowledge for accurate lifting/handling techniques can be helpful to prevent the CLBP in mothers of children with CP.
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http://dx.doi.org/10.1155/2020/8854435DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7787802PMC
December 2020

Management of refractory Acanthamoeba keratitis, two cases.

Parasitol Res 2021 Mar 6;120(3):1121-1124. Epub 2021 Jan 6.

Ocular Trauma and Emergency Department, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran.

Acanthamoeba keratitis is a serious infection of the eye that can result in permanent visual impairment or blindness, caused by free-living amoebae of the genus Acanthamoeba. Early diagnosis is necessary for effective treatment of Acanthamoeba keratitis. Acanthamoeba is abundant in nature and can be found in water, soil, and air. Acanthamoeba keratitis is usually diagnosed by culture from a scraping of the eye or by confocal microscopy. In this paper, two complicated Acanthamoeba keratitis cases are reported.
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http://dx.doi.org/10.1007/s00436-020-06997-6DOI Listing
March 2021

Anti-M antibody and ABO blood grouping discrepancy: a report of three cases with review of literature.

Hematol Transfus Cell Ther 2020 Dec 5. Epub 2020 Dec 5.

Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran. Electronic address:

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http://dx.doi.org/10.1016/j.htct.2020.09.150DOI Listing
December 2020

Repeatability and agreement of total corneal and sublayer pachymetry with 2 different algorithms of Fourier-domain optical coherence tomography in myopic and postphotorefractive keratectomy eyes.

J Cataract Refract Surg 2020 12;46(12):1644-1651

From the Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran.

Purpose: To investigate repeatability and agreement of total corneal and sublayer pachymetry with 2 different algorithms of Fourier-domain optical coherence tomography (OCT) in myopic and postphotorefractive keratectomy (PRK) eyes.

Setting: Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran.

Design: Prospective observational study.

Methods: Total corneal, epithelial, and stromal thicknesses were measured using RTVue-XR OCT with Pachymetry + Cpwr (6.0 mm algorithm) and PachymetryWide (9.0 mm algorithm) scan patterns. The repeatability of 25 zones of 9.0 mm map and 17 zones of 6.0 mm map and the agreement between measurements of these 2 algorithms were calculated.

Results: Ninety-five myopic and 117 post-PRK patients were evaluated. By the 9.0 mm algorithm, coefficient of variation (CoV) for total cornea was 2.33% or lesser and 2.49% or lesser and for epithelium was 5.14% or lesser and 5.18% or lesser; and by the 6.0 mm algorithm, CoV for total cornea was 1.80% or lesser and 2.59% or lesser and for epithelial thickness was 3.08% or lesser and 4.80% or lesser in myopic and post-PRK eyes, respectively. Bland-Altman mean difference for epithelial thickness was 0.69 or lesser and 1.16 or lesser and 95% limits of agreement for epithelial thickness was 6.81 or lesser and 8.56 or lesser in myopic and post-PRK eyes, respectively.

Conclusions: Good repeatability was seen in measurements of total corneal, stromal, and epithelial thicknesses by both algorithms. Agreement of the 2 algorithms in central zone was also good. However, large range of variation in paracentral thickness measurements did not allow us to consider these algorithms as interchangeable.
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http://dx.doi.org/10.1097/j.jcrs.0000000000000348DOI Listing
December 2020

A ten-year report of microbial keratitis in pediatric population under five years in a tertiary eye center.

J Ophthalmic Inflamm Infect 2020 Nov 27;10(1):35. Epub 2020 Nov 27.

Ocular Trauma and Emergency Department, Eye Research Center, Farabi eye hospital, Tehran University of Medical Sciences, Tehran, Iran.

Purpose: To report characteristics of microbial keratitis in pediatric patients under five years.

Methods: Patients with infectious keratitis under the age of 5 years were included in this retrospective cross-sectional study for ten years. All patients were admitted and corneal scraping was performed in 81 children. Fortified empiric antibiotic eye drops including cefazolin (50 mg/cc) and amikacin (20 mg/cc) were started and the antibiotic regimen was continued or changed according to culture results. In the case of fungal keratitis, topical voriconazole (10 mg/cc) or natamycin (50 mg/cc) and topical chloramphenicol (5 mg/cc) were started. A tectonic procedure was done when corneal thinning or perforation was present.

Results: Ninety-Three Patients between 1 to 60 months with a mean age of 33 ± 18 months old with corneal ulcer were included in the study. The most common risk factor was trauma (40.9%) followed by contact lens use (8.6%). Cultures were negative for microbial growth in 28 (30.1%) patients. The most common pathogens were S. epidermidis (10.8%) and P. aeruginosa (10.8%). Fluoroquinolone antibiotics (ciprofloxacin; 93.8% sensitivity) were the most potent antibiotic against bacterial pathogens. Forty-one patients underwent tectonic procedures, which the most common ones were cyanoacrylate glue 18.3% followed by keratoplasty 16.1%.

Conclusion: This study emphasizes the role of trauma as the primary cause and S. epidermidis as the most frequent microorganism in pediatric keratitis; according to antibiogram results and poor cooperation of patients under five years, monotherapy with fluoroquinolones could be a good regimen in small non-central lesions without thinning.
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http://dx.doi.org/10.1186/s12348-020-00227-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7695777PMC
November 2020

Artemisia biennis Willd.: Anti-Nociceptive effects and possible mechanisms of action.

J Ethnopharmacol 2021 Mar 21;268:113604. Epub 2020 Nov 21.

Department of Physiology, School of Medicine, Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran. Electronic address:

Ethnopharmacological Relevance: Artemisia biennis Willd. (Dermane in Persian) has been used as an antinociceptive remedy in Iranian folkloric medicine.

Objective: The aim of the present study was to evaluate the anti-nociceptive effects of Artemisia biennis Willd. aerial part essential oil (ABAEO) on male Swiss mice.

Materials And Methods: Nociceptive pain techniques including acetic acid-induced writhing (AAIW), formalin-induced paw licking (FPL), glutamate-induced paw licking (GPL), and tail-flick (TF) models were applied. We assessed opioid and L-arginine-NO-cGMP-KATP pathways to detect the possible anti-nociceptive properties of ABAEO. In addition, neuropathic pain was induced by the cervical spinal cord contusion model.

Results: ABAEO (120 mg/kg) had a significant anti-nociceptive activities in comparison to the control animals (p < 0.05) in the AAIW, TF, GPL, and FPL assays. The selective opioid antagonist (naloxonazine) administration in the AAIW test alleviated the anti-nociceptive effect of ABAEO (p < 0.05). L-arginine, methylene blue, and glibenclamide treatment prevented the ABAEO anti-nociceptive effects (p < 0.05); however, sodium nitroprusside could profoundly potentiate the ABAEO-associated antinociception in the FPL (phase II) test (p < 0.05). In nociceptive pain models, Cr (one of the main constituents of ABAEO) showed significant anti-nociceptive effects (p < 0.05). Moreover, the von Frey results indicated that ABAEO could attenuate mechanical allodynia in mice.

Conclusion: Our observation revealed the anti-nociceptive effects of ABAEO in male mice. These effects could include, at least in part, modulating glutamatergic mechanisms via opioid systems. Our data output also indicates activating the L-arginine-NO-cGMP-KATP system in ABAEO anti-nociceptive activities.
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http://dx.doi.org/10.1016/j.jep.2020.113604DOI Listing
March 2021

Effects of different autophagy inhibitors on sensitizing KG-1 and HL-60 leukemia cells to chemotherapy.

IUBMB Life 2021 Jan 17;73(1):130-145. Epub 2020 Nov 17.

Hematology Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran.

A little number of current autophagy inhibitors may have beneficial effects on the acute myeloid leukemia (AML) patients. However, there is a strong need to figure out which settings should be activated or inhibited in autophagy pathway to prevail drug resistance and also to improve current treatment options in leukemia. Therefore, this study aimed to compare the effects of well-known inhibitors of autophagy (as 3-MA, BafA1, and HCQ) in leukemia KG-1 and HL-60 cells exposed to arsenic trioxide (ATO) and/or all-trans retinoic acid (ATRA). Cell proliferation and cytotoxicity of cells were examined by MTT assay. Autophagy was studied by evaluating the development of acidic vesicular organelles, and the autophagosomes formation was investigated by acridine orange staining and transmission electron microscopy. Moreover, the gene and protein expressions levels of autophagy markers (ATGs, p62/SQSTM1, and LC-3B) were also performed by qPCR and western blotting, respectively. The rate of apoptosis and cell cycle were evaluated using flow cytometry. We compared the cytotoxic and apoptotic effects of ATO and/or ATRA in both cell lines and demonstrated that some autophagy markers upregulated in this context. Also, it was shown that autophagy blockers HCQ and/or BafA1 could potentiate the cytotoxic effects of ATO/ATRA, which were more pronounced in KG-1 cells compared to HL-60 cell line. This study showed the involvement of autophagy during the treatment of KG-1 and HL-60 cells by ATO/ATRA. This study proposed that therapy of ATO/ATRA in combination with HCQ can be considered as a more effective strategy for targeting leukemic KG-1 cells.
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http://dx.doi.org/10.1002/iub.2411DOI Listing
January 2021

Effect of a Health-Education Program Based on the BASNEF Model of Overall Sexual Health Satisfaction and Satisfaction with Quality of Sexual Relationship among Women with Infertility.

Int J Womens Health 2020 4;12:975-982. Epub 2020 Nov 4.

Population Health Research Group, Health Metrics Research Center, Iranian Institute for Health Sciences Research, ACECR, Tehran, Iran.

Introduction: Female infertility is increasing in Iran, encompassing all dimensions of women's lives. One of the most important effects of infertility is on sexual function. All women have the right to sexual health. Using educational models, one can see a positive effect on women's sexual function. The present study aimed to investigate the effect of an education program based on the beliefs, attitudes, subjective norms, and enabling factors (BASNEF) model on promoting overall sexual health satisfaction and satisfaction with the quality of sexual relationships among women with infertility.

Methods: This was a quasiexperimental study conducted on 80 infertile women referred to an infertility center affiliated with Tehran University of Medical Sciences, in Tehran, Iran in 2016-2017. Infertile women with primary infertility were divided into experimental and control groups based on odd and even days. The intervention was carried out based on the BASNEF model over four sessions. Data were collected using a demographic questionnaire, the BASNEF questionnaire, and items on overall sexual health satisfaction and satisfaction with their sexual relationship. The questionnaires were completed before and 4 and 8 weeks after the intervention in the two groups. Friedman, χ, and -tests were used for data analysis.

Results: After the educational intervention, BASNEF values and overall sexual function and satisfaction with the quality of sexual relationships had significantly improved in the experimental group (<0.05), while changes were not significant in the control group.

Conclusion: The results showed that the BASNEF model-based educational invention improved overall sexual health and satisfaction among intertitle women. Indeed, it seems that using theory-based educational interventions in infertility centers might be helpful.
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http://dx.doi.org/10.2147/IJWH.S248734DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7651995PMC
November 2020

Potential of therapeutic plasmapheresis in treatment of COVID-19 patients: Immunopathogenesis and coagulopathy.

Transfus Apher Sci 2020 Dec 2;59(6):102993. Epub 2020 Nov 2.

Hematology, Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran; Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran. Electronic address:

Therapeutic plasmapheresis (TP) is the process of the separation and removal of plasma from other blood components and is considered as an adjunctive treatment strategy to the discarded abnormal agent in the management of respiratory viral pandemics. This article reviews the mechanisms of immunopathogenesis and coagulopathy induced by SARS-CoV-2 and the potential benefits of TP as adjunctive treatment in critically COVID-19 patients.
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http://dx.doi.org/10.1016/j.transci.2020.102993DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7605792PMC
December 2020

Synthesis, characterization, and in vitro evaluation of the starch-based α-amylase responsive hydrogels.

J Cell Physiol 2021 May 5;236(5):4066-4075. Epub 2020 Nov 5.

Hematology, Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran.

Controlled-release drug delivery systems are promising platforms in medicine. Among various types of material in drug delivery, hydrogels are interesting ones. They are water-soluble and tissue compatible polymers with a high capacity to carry and release drugs in a controllable manner. In this study, we introduce the synthesis, characterization, and application of an α-amylase responsive hydrogel in controlled drug delivery. The newly synthesized starch-based hydrogels structurally characterized by means of Fourier-transform infrared spectroscopy and scanning electron microscopy. A proapoptotic drug, doxorubicin, was loaded into the hydrogels and the controlled release of the drug was assessed in the presence of α-amylase and ultimately it was evaluated to controlled-drug release in vitro and subsequently in killing cancer cells. Our results highlight the effectiveness of temporal drug delivery using α-amylase responsive hydrogels in killing cancer cells.
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http://dx.doi.org/10.1002/jcp.30148DOI Listing
May 2021

Changes in Corneal Subbasal Nerves after Punctal Occlusion in Dry Eye Disease.

Curr Eye Res 2020 Oct 22:1-7. Epub 2020 Oct 22.

Ocular Surface Imaging Center, Cornea and Refractive Surgery Service, Massachusetts Eye and Ear Infirmary, Department of Ophthalmology, Harvard Medical School , Boston, Massachusetts, United States.

Purpose: To evaluate corneal subbasal nerve plexus by in vivo confocal microscopy (IVCM) following punctal occlusion in patients with moderate to severe dry eye disease (DED).

Materials And Methods: Patients with grade 3 or 4 severity of DED based on Delphi Panel dry eye severity grading scheme were enrolled in the study. Permanent inferior punctal occlusion was performed. A comprehensive ophthalmic evaluation, including Ocular Surface Disease Index (OSDI) questionnaire, tear break-up time (TBUT), corneal fluorescein staining, conjunctival Rose bengal staining, Schirmer's test, and corneal sensation by Cochet-Bonnet esthesiometry, were performed at baseline, and 1 and 3 months after punctal occlusion. Furthermore, density and number of corneal subbasal nerves were evaluated by IVCM.

Results: Forty-one eyes of 23 patients with a mean age of 46.3 ± 9.0 years were enrolled. Corneal fluorescein staining, Rose bengal staining, and TBUT significantly improved at 3 months following punctal occlusion ( < .015). Corneal esthesiometry significantly increased at both postoperative visits ( < .03), and OSDI scores improved only at 3-month follow-up ( < .005). Nerve density and total number significantly increased 3 months after punctal occlusion ( < .045). Baseline nerve density had significant correlations with TBUT, fluorescein staining, Rose bengal staining ( < .012), but not with esthesiometry, Schirmer scores, or OSDI scores ( > .329).

Conclusions: Corneal subbasal nerve density and total number increased following punctal occlusion in patients with moderate to severe DED. These findings were associated with improvements in corneal sensation, and signs and symptoms of DED. This emphasizes the effect of punctal occlusion in regeneration of corneal subbasal nerve plexus.
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http://dx.doi.org/10.1080/02713683.2020.1833349DOI Listing
October 2020

PD-1 Expression on CD8+CD28- T cells within inflammatory synovium is associated with Relapse: A cohort of Rheumatoid Arthritis.

Immunol Lett 2020 Dec 15;228:76-82. Epub 2020 Oct 15.

Stem Cell Research Center, Golestan University of Medical Sciences, Gorgan, Iran; Department of Immunology, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran. Electronic address:

Defect in T lymphocyte homeostasis could implicate initiation and development of rheumatoid arthritis (RA). Since PD-1 plays a key role in the regulation of T lymphocytes, its expression pattern in various CD8+ T cell subsets could be so effective in RA pathogenesis. Here, we investigated the expression of PD-1 and CXCR3 on CD8+CD28- T cells in association with the IFN-γ levels in patients with RA. A total of 42 RA patients, including 10 newly-diagnosed (ND) and 32 relapsed (RL) cases and also 20 healthy donors were enrolled. Phenotypic characterization of CD8+ T cells derived from peripheral blood (PB) and synovial fluid (SF) was performed by flow cytometry. The plasma and SF IFN-γ levels were also assessed by ELISA. The frequency of CD8+CD28- T cells showed no significant differences between patients and controls while its higher levels were observed in PB, versus SF of RL patients. Relapsed patients also showed higher CXCR3 and especially PD-1 expression on their CD8+CD28- T cells. The IFN-γ concentration was elevated in SF of ND patients while its plasma level was significantly lower in RL subgroup than controls. Although PD-1 could induce immune suppression in effector T cells, it is upregulated during inflammation and its overexpression on CD8+CD28- T cells within inflammatory synovium is associated with severity of disease in our cohort of RA patients.
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http://dx.doi.org/10.1016/j.imlet.2020.10.005DOI Listing
December 2020

Variants in Intron 4 of PD-1 Gene are Associated with the Susceptibility to SLE in an Iranian Population.

Iran J Immunol 2020 Sep;17(3):204-214

Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.

Background: Programmed cell death protein 1 (PD-1) is a negative co-stimulatory molecule with immunomodulatory properties. Recently, PD-1 gene defects have attracted attention in the pathogenesis of SLE.

Objective: Here, we assessed the association of PD-1 gene polymorphisms in intron 4 and haplotypes with the susceptibility to SLE.

Methods: Seventy-six SLE patients and 159 healthy controls were included. We screened the polymorphisms by amplifying the intron 4 of the PD-1 gene with the specific primers followed by sequencing.

Results: Two distinct SNPs were identified (rs6705653 and rs41386439) within the intron 4 of the PD-1 gene. The AA genotype of +7499 (G/A) SNP was associated with the higher risk of SLE [OR=3.31, 95% CI (1.25-8.76), p-value=0.045], while A allele was identified as a risk allele [OR=1.75, 95% CI (1.10-2.76), p-value=0.015]. However, no significant association was observed between the allele and the genotype frequencies of +7209 (C/T) polymorphic region of the PD-1 gene and susceptibility to SLE. Haplotype analysis showed the significantly higher presence of H2 haplotype (AC; +7499/+7209) [OR=1.70, 95% CI (1.24-2.33), p-value=0.0012] in SLE patients.

Conclusion: To the best of our knowledge, this is the first report of the significant association of PD-1 +7499 (G/A) SNP with the SLE susceptibility and the first detection of both polymorphic loci in a population from Iran. However, more investigations are necessary to confirm these findings.
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http://dx.doi.org/10.22034/iji.2020.83046.1610DOI Listing
September 2020

Blood supply sufficiency and safety management in Iran during the COVID-19 outbreak.

Vox Sang 2021 Feb 30;116(2):175-180. Epub 2020 Sep 30.

Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.

Background: COVID-19 first appeared in Iran on 19 February 2020, and then spread rapidly over the country. In this article, we review the action plan of the Iranian Blood Transfusion Organization with respect to this disease.

Method And Materials: We collected data on blood donations and RBC inventory for the first 8 weeks of the outbreak. We also evaluated the trend of blood donations and RBC inventory and compared them with the data of the past year. We include a summary of actions taken by the National Committee on Management of COVID-19 outbreak.

Results: Blood donations decreased from 33 275 to 23 465 units during the first 2 weeks of the outbreak with a corresponding decrease in the RBC inventory. But after that, donations gradually increased from 23 465 to 29 665 units. RBC inventory levels improved at the same time. Then, the Iranian New Year's holiday resulted in another downward trend. After the holiday, blood donations revived, along with the RBC inventory.

Discussion: Although it appears that this virus cannot be transmitted through transfusion, changes in lifestyle had a significant impact on reducing blood supply. Following implemented measures, we saw an upward trend in blood donations and an adequate supply of RBC units in blood centres, helped by a reduction in demand by hospitals. Blood centres need to be more prepared to manage future viral disasters, especially in case of transfusion-transmissible infections.
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http://dx.doi.org/10.1111/vox.13012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7537134PMC
February 2021

Longitudinal Epithelial Thickness Profile Changes 18 Months After Photorefractive Keratectomy.

Cornea 2020 Sep 16. Epub 2020 Sep 16.

Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran.

Purpose: Long-term evaluation of corneal epithelial thickness (ET) profile changes after photorefractive keratectomy (PRK) using Fourier-domain anterior segment optical coherence tomography.

Methods: Three hundred twenty-six eyes of 163 patients were included in this prospective observational study. The corneal epithelial map was obtained across a 9-mm diameter area of the cornea before and up to 27 months after surgery. ET was assessed in 25 sectors and 4 annular zones (central 2 mm, paracentral 2-5 mm, midperipheral 5-7 mm, and peripheral 7-9 mm).

Results: There was a significant reduction in mean ET in all zones 1 month after PRK. Subsequently, ET increased in all annular zones. The change in mean ET became stable in the midperipheral and peripheral zones at 3 to 6 months and in the central zone at 12 months, and it continued to increase in the paracentral zone even after 18 months after surgery. The ET was 3.40 μm and 4.05 μm in the central and paracentral zones at 6 months, respectively. Postoperative spherical equivalent changed significantly only from 1 to 3 months (P < 0.04). There was a significant correlation between postoperative spherical equivalent at month 1 and ET change in the paracentral and midperipheral zones (P < 0.027).

Conclusions: There is a significant reduction in ET 1 month after myopic PRK with a gradual thickening thereafter until it reaches stability at 12 months in the central zone. However, it continues to change even after 18 months in the paracentral zone. The greatest thickening is in the paracentral zone, followed by the central zone.
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http://dx.doi.org/10.1097/ICO.0000000000002521DOI Listing
September 2020

COVID-19: The Immune Responses and Clinical Therapy Candidates.

Int J Mol Sci 2020 Aug 3;21(15). Epub 2020 Aug 3.

Storr Liver Centre, Westmead Institute for Medical Research, Westmead Hospital and University of Sydney, Sydney, NSW 2145, Australia.

The pandemic of coronavirus disease 2019 (COVID-19), with rising numbers of patients worldwide, presents an urgent need for effective treatments. To date, there are no therapies or vaccines that are proven to be effective against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Several potential candidates or repurposed drugs are under investigation, including drugs that inhibit SARS-CoV-2 replication and block infection. The most promising therapy to date is remdesivir, which is US Food and Drug Administration (FDA) approved for emergency use in adults and children hospitalized with severe suspected or laboratory-confirmed COVID-19. Herein we summarize the general features of SARS-CoV-2's molecular and immune pathogenesis and discuss available pharmacological strategies, based on our present understanding of SARS-CoV and Middle East respiratory syndrome coronavirus (MERS-CoV) infections. Finally, we outline clinical trials currently in progress to investigate the efficacy of potential therapies for COVID-19.
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http://dx.doi.org/10.3390/ijms21155559DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7432271PMC
August 2020

Hydatid Cyst of Inferior Rectus.

Ophthalmic Plast Reconstr Surg 2020 Jul 23. Epub 2020 Jul 23.

Eye Research Center, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran.

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http://dx.doi.org/10.1097/IOP.0000000000001749DOI Listing
July 2020

Urolithiasis, growth and blood pressure in childhood: A case-control study.

Clin Nutr ESPEN 2020 08 27;38:74-79. Epub 2020 Jun 27.

Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran. Electronic address:

Background & Aims: Kidney stone is a life-threatening disease with subsequent complications, especially in children who overtake this disease in early ages. This study assessed the effect of kidney stone history on current blood pressure and growth parameters of children.

Methods: A two hundred children with (n = 100) and without (n = 100) a history of urolithiasis from Pediatric Urology clinics at the Aliasghar hospital, Tehran, Iran, participated in this case-control study. Several demographic data, height, weight, body mass index (BMI), BMI for age and blood pressure index were measured as the primary outcomes.

Results: type of birth and infancy feeding, carbohydrate and energy intake were significantly different between the two groups (P = 0.008, 0.002, 0.03 and < 0.001 respectively). Ordinal logistic regression analysis showed that the current weight (P = 0.001) and BMI for age (P = 0.02) of the stone formers were lower than the non-stone formers, while no significant association found between the blood pressure or current height and the history of urolithiasis.

Conclusion: Our findings suggest that childhood urolithiasis has no significant effect on growth failure and blood pressure in childhood but it has a little impact on weight and BMI for age in older ages.
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http://dx.doi.org/10.1016/j.clnesp.2020.06.005DOI Listing
August 2020

Modified Encircling Scleral Buckle Technique Without Subretinal Fluid Drainage or Retinopexy.

Ophthalmol Ther 2020 Sep 14;9(3):641-651. Epub 2020 Jul 14.

Department of Ophthalmology, Southern California Permanente Medical Group, Baldwin Park, CA, USA.

Introduction: Scleral buckling (SB) tends to be more challenging and time-consuming for compared to the pars plana vitrectomy for repairing rhegmatogenous retinal detachments (RRDs). This study characterizes a novel and simplified technique for SB.

Methods: In this single-masked randomized interventional study, patients with RRDs who were eligible for SB were randomly assigned to either the standard (S) or modified (M) technique of SB. In the modified approach, neither intraoperative break localization nor cryopexy or subretinal fluid drainage was done. A large tire (276/279) was placed where preoperative retinal breaks had been localized with a 240 encircling band placed for support of the remaining retina. Patients were followed for 12 months and the primary outcomes were differences between the surgical groups in operative time, anatomical success, visual acuity, and complication rate.

Results: Thirty-six eyes were included in the study (18 in each arm). There were no differences in baseline patient demographics or characteristics including gender, age, lens and macular status, preoperative vision, and symptom duration. The mean length of surgery was 72.2 ± 13.2 and 56.2 ± 9.5 min in groups S and M, respectively (P = 0.001). Complete retinal reattachment at the end of month 12 after single surgery was 80.6% overall; 77.8% (14/18) in group S and 83.3% (15/18) in group M (P > 0.999). After 12 months, both groups achieved similar final best-corrected visual acuity (BCVA): 0.26 ± 0.23 and 0.23 ± 0.17 logMAR in groups S and M, respectively (P = 0.231). Controlling for preoperative BCVA on ANCOVA testing, there were no significant differences in visual improvement between the two groups [F (1,26) = 0.02, P = 0.966 (95% CI) - 0.128 to 0.123)]. Scleral perforation (2:1), vitreous hemorrhage (3:2), and transient rise of intraocular pressure (3:4) all occurred at a low and similar rate between the two groups (S:M).

Conclusion: Modified SB technique was non-inferior compared to the standard approach for anatomical and visual outcomes. Shortening surgical time while maintaining low complication rates makes this an appropriate approach to SB, especially for vitreoretinal surgery trainees.
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http://dx.doi.org/10.1007/s40123-020-00279-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7406596PMC
September 2020

Orbital anatomical parameters affecting outcome of deep lateral orbital wall decompression.

Eur J Ophthalmol 2020 Jul 6:1120672120941433. Epub 2020 Jul 6.

Division of Orbital and Ophthalmic Plastic Surgery, Jules Stein Eye Institute, University of California-Los Angeles, Los Angeles, CA, USA.

Purpose: To determine orbital anatomical parameters that affect surgical outcome of deep lateral orbital wall decompression.

Methods: Twenty orbits of 20 patients with moderate proptosis (maximum 25 mm) due to thyroid eye disease who were stable for at least 6 months were included in this prospective cohort study. Four parameters including lateral orbital wall distance (LOWD), removable surface area (RSA), removable surface length (RSL), and sphenoid door jamb thickness (SDJ) were evaluated by computed tomography (CT) scan prior to surgery in these patients. Deep lateral orbital wall decompression without fat removal was done in all patients. Relationship between all these factors and proptosis reduction, 6 months after surgery was assessed.

Results: Mean proptosis before decompression surgery (mean ± SD) was 23.22 ± 1.19 mm and reduction in Hertel exophthalmometry (mean ± SD) was -3.27 ± 1.03 mm ( = 0.006). Pearson correlation showed that LOWD, RSL, and SDJ had statistically significant correlation with proptosis reduction ( = 0.017, 0.002, and 0.001, respectively.). Linear multivariate regression analysis showed SDJ as the only independent factor in predicting surgical outcome.

Conclusion: several orbital anatomical factors have significant role in predicting surgical outcome following deep lateral wall orbital decompression. Among these parameters, SDJ is an independent factor, whereas LOWD and RSL are dependent factors. Preoperative assessment of the bony sphenoid anatomy may be helpful in predicting the amount of potential decompression.
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http://dx.doi.org/10.1177/1120672120941433DOI Listing
July 2020

New marsupialization technique in endolymphatic sac surgery.

Laryngoscope Investig Otolaryngol 2020 Jun 26;5(3):546-551. Epub 2020 May 26.

Skull Base Research Center The Five Senses Institute, Iran University of Medical Sciences Tehran Iran.

Objectives: The aim of the present study was to describe and evaluate the results of a new technique in endolymphatic sac decompression surgery.

Methods: Forty-three patients with intractable unilateral Meniere's disease were selected. Endolymphatic sac was identified after simple mastoidectomy, and its lateral layer was incised, using a sickle knife. Outer layer of the sac was turned around and placed under the anterior bony border.

Results: Mean duration of the follow-up was 24 months. Mean tinnitus handicap index, pure tone average (PTA) on thresholds at 500, 1000, 2000, and 4000 Hz, mean speech reception threshold, mean speech discrimination score, hearing stage, and mean vertigo score before and after surgery were evaluated.

Conclusion: The new marsupialization technique with anterior bony border is a safe and effective way to improve tinnitus, vertigo, and ear fullness among these patients. According to PTA and hearing stage, this surgery can control progressive hearing loss.

Level Of Evidence: 3.
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http://dx.doi.org/10.1002/lio2.403DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7314464PMC
June 2020