Publications by authors named "Sadia Afzal"

3 Publications

  • Page 1 of 1

Microwave-assisted synthesis and evaluation of type 1 collagen-apatite composites for dental tissue regeneration.

J Biomater Appl 2018 07 2;33(1):103-115. Epub 2018 May 2.

4 Imam Abdulrahman Bin Faisal University College of Dentistry, Dammam, Saudi Arabia.

The aim was to develop an economical and biocompatible collagen-based bioactive composite for tooth regeneration. Acid-soluble collagen was extracted and purified from fish scales. The design was innovated to molecularly tailor the surface charge sites of the nano-apatite providing chemical bonds with the collagen matrix via microwave irradiation technique. The obtained collagen was identified by sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis. The composites were characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis/differential scanning calorimetry, and scanning electron microscopy. MC3T3-E1 cell lines were used to assess the biological effects of these materials by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetra zolium bromide (MTT) assay. Indirect contact test was performed by extracting representative elutes in cell culture media and sulforhodamine B analysis was performed. Chorioallantoic membrane assay was conducted to define the new vessels formation behavior. The purity of collagen extracts was determined and showed two α-chains, i.e. the characteristic of type I collagen. Fourier transform infrared spectroscopy showed the characteristic peaks for amide I, I, III, and phosphate for collagen and composites. Scanning electron microscopy images showed three-dimensional mesh of collagen/apatite nano-fibers. Nontoxic behavior of composites was observed and there were graded and dose-related effects on experimental compounds. The angiogenesis and vessels formation behavior were observed in bioactive collagen composite. The obtained composites have potential to be used for tooth structure regeneration.
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July 2018


Acta Crystallogr Sect E Struct Rep Online 2012 Nov 20;68(Pt 11):o3142. Epub 2012 Oct 20.

Materials Chemistry Laboratory, Department of Chemistry, GC University, Lahore 54000, Pakistan.

In the title compound C(11)H(17)NO(4)S, an intra-molecular O-H⋯O hydrogen bond forms an S(8) ring and determines the conformation of the bis-(2-hy-droxy-eth-yl) segment of the mol-ecule, holding the two CH(2)CH(2)OH groups close to coplanar (r.m.s. deviation = 0.185 Å). In the crystal, O-H⋯O hydrogen bonds link the mol-ecules into zigzag chains along the b axis. Weaker additional C-H⋯O and C-H⋯π contacts generate a three dimensional network, with mol-ecules stacked along the b-axis direction.
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November 2012

Serum estrogen and interleukin-6 levels in postmenopausal female osteoarthritis patients.

Pak J Pharm Sci 2011 Apr;24(2):217-9

Department of Biochemistry, University of Karachi, Karachi-75270, Pakistan.

The objective of the present study is to determine whether the levels of endogenous estrogen and interleukin-6 (IL-6) are associated with osteoarthritis (OA) in postmenopausal women. Forty-three patients who were suffering from osteoarthritis were studied and twenty control normal subjects having no symptoms of osteoarthritis disease, were also included in the study. The estrogen hormone and interleukin-6 were measured in the serum by Enzyme linked immunosorbent assay technique. Interleukin-6 and estrogen both were detected in serum. In osteoarthritis patients IL-6 levels were high as compared to control subjects (P<0.001) and estrogen levels in OA postmenopausal females was decreased significantly (p<0.01) when compared with control subjects. There was a minimal inverse correlation between IL-6 and estrogen (P<0.05) in the sera of postmenopausal osteoarthritic women. These results indicate that estrogen deficiency after menopause may cause alterations of IL-6 in postmenopausal osteoarthritic patients.
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April 2011