Publications by authors named "S Tso"

111 Publications

Measuring the K of Protein-Ligand Interactions Using Microscale Thermophoresis.

Methods Mol Biol 2021 ;2263:161-181

Departments of Biophysics and Microbiology, UT Southwestern Medical Center, Dallas, TX, USA.

Microscale thermophoresis (MST) has become a widely used technique to determine the K or EC of protein-ligand interactions. The method exploits the tendency of macromolecules to migrate along a thermal gradient (i.e., thermophoresis). Differences in thermophoresis as a function of the liganded state of a macromolecule can be measured and assembled into a binding curve that can be analyzed to yield K. In this protocol, we outline a simple experiment designed for new MST users, with the goal of using readily available, inexpensive materials to plan, execute, and analyze an MST experiment.
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http://dx.doi.org/10.1007/978-1-0716-1197-5_6DOI Listing
January 2021

A multi-laboratory benchmark study of isothermal titration calorimetry (ITC) using Ca and Mg binding to EDTA.

Eur Biophys J 2021 Apr 16. Epub 2021 Apr 16.

CIQUP, Department of Chemistry and Biochemistry, Faculty of Sciences, University of Porto, Porto, Portugal.

A small-scale ITC benchmarking study was performed involving 9 biophysics laboratories/facilities, to evaluate inter-laboratory and intra-laboratory basal levels of uncertainty. Our prime goal was to assess a number of important factors that can influence both the data gathered by this technique and the thermodynamic parameter values derived therefrom. In its first part, the study involved 5 laboratories and 13 different instruments, working with centrally prepared samples and the same experimental protocol. The second part involved 4 additional laboratories and 6 more instruments, where the users prepared their own samples according to provided instructions and did the experiments following the same protocol as in the first part. The study design comprised: (1) selecting a minimal set of laboratories; (2) providing very stable samples; (3) providing samples not requiring preparation or manipulation; and (4) providing a well-defined and detailed experimental protocol. Thus, we were able to assess: (i) the variability due to instrument and data analysis performed by each user on centrally prepared samples; (ii) the comparability of data retrieved when using 4 different software packages to analyze the same data, besides the data analysis carried out by the different users on their own experimental results; and (iii) the variability due to local sample preparation (second part of the study). Individual values, as well as averages and standard deviations for the binding parameters for EDTA-cation interaction, were used as metrics for comparing the equilibrium association constant (logK), enthalpy of interaction (ΔH), and the so-called "stoichiometry" (n), a concentration-correction factor.
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http://dx.doi.org/10.1007/s00249-021-01523-7DOI Listing
April 2021

Ribonucleic acid (RNA) COVID-19 vaccine associated cutaneous adverse drug events: a case series of two patients.

Clin Exp Dermatol 2021 Apr 9. Epub 2021 Apr 9.

Jephson Dermatology Centre, South Warwickshire NHS Foundation Trust, Warwick, CV34 5BW, United Kingdom.

Vaccines are biological preparations that enable their recipients to acquire immunity to a specific infectious disease. All vaccines can associate with cutaneous adverse drug events. Ribonucleic acid (RNA) vaccine was first tested in humans in COVID-19 prevention trials in 2020. The Pfizer-BioNTech COVID-19 vaccine utilises lipid nanoparticles, which act as a vector for the embedded mRNA. In a phase III clinical trial, it was found that local reactions at the injection site are the commonest side effect (84.7%). Other adverse reactions included fatigue, headache, muscle ache, chills, joint pain and fever. Jedlowski and Jedlowski (2021) reported a case of recurrent morbilliform rash that developed 48 hours following administration of the Pfizer-BioNTech COVID-19 vaccine on two separate occasions, 21 days apart. We hereby report two patients presented with cutaneous adverse drug events following RNA vaccination (Pfizer-BioNTech COVID-19 vaccine).
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http://dx.doi.org/10.1111/ced.14673DOI Listing
April 2021

Erythroderma (exfoliative dermatitis) part two: energy homeostasis and dietetic management strategies.

Clin Exp Dermatol 2021 Apr 5. Epub 2021 Apr 5.

Warwick Medical School, University of Warwick, CV4 7AL, United Kingdom.

Erythroderma (exfoliative dermatitis) associates with important metabolic changes that include an enhancement in energy expenditure. The key components to total energy expenditure (TEE) include basal metabolic rate (~68% of TEE), physical activity (~22% of TEE) and thermic effect of food (~10% of TEE). In the erythrodermic state, there are likely multiple contributors to the increase in basal metabolic rate such as 'caloric drain' resulting from increased evaporation of water from enhanced transepidermal water loss, increased activity of the cardiovascular system (including high-output cardiac failure), increased non-shivering thermogenesis and hormonal changes (such as hypercortisolaemia). A change to patient's level of physical activity and appetite as a result of ill health status may further impact on their total energy expenditure and energy consumption. In part two of this two part concise review, we explore the key constituents of energy homeostasis, the potential mechanisms impacting on energy homeostasis in erythroderma and suggest much needed dietetic management strategies for this important condition.
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http://dx.doi.org/10.1111/ced.14667DOI Listing
April 2021

Erythroderma (exfoliative dermatitis). Part 1: underlying causes, clinical presentation and pathogenesis.

Clin Exp Dermatol 2021 Feb 27. Epub 2021 Feb 27.

Department of Haematology, Tan Tock Seng Hospital, Singapore, Singapore.

Erythroderma (exfoliative dermatitis), first described by Von Hebra in 1868, manifests as a cutaneous inflammatory state, with associated skin barrier and metabolic dysfunctions. The annual incidence of erythroderma is estimated to be 1-2 per 100 000 population in Europe with a male preponderance. Erythroderma may present at birth, or may develop acutely or insidiously (due to progression of an underlying primary pathology, including malignancy). Although there is a broad range of diseases that associate with erythroderma, the vast majority of cases result from pre-existing and chronic dermatoses. In the first part of this two-part concise review, we explore the underlying causes, clinical presentation, pathogenesis and investigation of erythroderma, and suggest potential treatment targets for erythroderma with unknown causes.
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http://dx.doi.org/10.1111/ced.14625DOI Listing
February 2021