Publications by authors named "S I Alekperov"

16 Publications

[Multifocal electroretinography in the study of focal and diffuse damage to the rabbit retina].

Vestn Oftalmol 2020 ;136(4):47-56

State Research and Experimental Institute of Military Medicine, Saint Petersburg, Russia.

Purpose: To evaluate the possibility of using the system "Neuro-ERG" (with a module for multifocal ERG) in the study of focal and diffuse pathology in laboratory animals (rabbits).

Material And Methods: Focal retinal damage was modelled in 5 eyes of 5 rabbits by singular laser pulses (532 nm, 100 ms, power 30, 60, 100, 150 and 200 mW) and diffuse retinal damage was modelled in 5 eyes of 5 rabbits by exposure to polychromatic light for 14 days (9500 lm, 6400 K, 230 mW/cm2, 8 h/day). The pair of eyes and areas of intact retina in the eyes with focal retinal damage were used for control. Multifocal electroretinography (mfERG) was recorded using the «Neuro-ERG» system (Neurosoft, Russia) before exposure, after 1 hour (in focal damage model), and 1, 7 and 14 days after exposure. In addition, three-time recording of mfERG was made before and after the experiments. Analysis included the amplitude and time characteristics of mfERG components, as well as the level of reproducibility of mfERG at each recording.

Results: In the modeling of focal damage of the rabbit retina, significant changes in mfERG (pattern stimulus consisted of 61 hexagons) were detected when the retinal damage area was more than 170 µm in diameter or more than 35% of the hexagon area in the pattern-stimulus. A significant moderate inverse correlation (0.52<<0.71, <0.01) was found between the damage area and the amplitude P1 and density of the bioelectric response of the P1 component. When modeling diffuse damage, significant changes in mfERG (an increase of implict-time of P1 and a decrease in the amplitude and density of the bioelectric response of the P1 component) were detected on the 7th day after the beginning of exposure. Variability of mfERG recording on each registration averaged 5%.

Conclusion: Multifocal ERG registration systems, including «Neuro-ERG» (Neurosoft, Russia), can be used in experimental studies of vitreoretinal pathology with rabbits as biological objects.
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http://dx.doi.org/10.17116/oftalma202013604147DOI Listing
August 2020

[Acute ocular lesions after exposure to electromagnetic radiation of ultrahigh frequency (an experimental study)].

Vestn Oftalmol 2019 ;135(4):41-49

State Research and Experimental Institute of Military Medicine, 4 Lesoparkovaya St., Saint Petersburg, Russian Federation, 195043.

Purpose: To investigate the clinical and morphological manifestations of ocular lesions resulting from acute exposure to microwave radiation (MR).

Material And Methods: Twenty-four rabbits were included in the study and divided into four equal groups according to MR exposure time (15, 30, 45, 60 s). The right eyes of rabbits were exposed to MR of 3.97 GHz and energy density of 1.0 W/cm. The sham control group consisted of six animals. The exposed (right) and the paired (left) eye were studied for clinical and morphological changes, content of proinflammatory cytokines IL-1β, IL-6 and TNF-α in the anterior chamber and in the vitreous body after exposure to MR.

Results: Significant dose-dependent changes in the structure of the exposed eye were revealed. Formation of microwave cataract was noted at the MR exposure time of 15 seconds and more. Partial or complete de-epithelialization, stromal edema, endothelial damage and inflammatory infiltration in the cornea, effusion of protein and cellular reaction in the aqueous humor were detected after MR exposure of 30, 45 and 60 seconds. Cellular reaction in the vitreous body was observed after exposure time of 45 and 60 seconds. Exposure to MR for up to 1 minute did not lead to visible clinical or morphological (traditional methods of examination) damage of the retina and optic nerve within 24 hours. Significantly higher content of proinflammatory cytokines IL-1ß, IL-6 and TNF-α in the aqueous humor and vitreous body was revealed in animals exposed to MR for 45 and 60 seconds.

Conclusion: Acute exposure of the organ of vision to electromagnetic microwave radiation can lead to adverse dose-dependent effects not only in the lens, but also in other structures of the eye.
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http://dx.doi.org/10.17116/oftalma201913504141DOI Listing
October 2019

[Mirizzi syndrome management (in Russian only)].

Khirurgiia (Mosk) 2019 (3):42-47

Martynov Chair of Hospital-Based Surgery #1, Sechenov First Moscow State Medical University of Ministry of Health of Russia Moscow, Russia, Vorokhobov Munitsipal Clinical Hospital #67 of Moscow Healthcare Department, Moscow, Russia.

Aim: To assess an effectiveness of complex preoperative diagnosis, conservative treatment, minimally invasive biliary decompression for Mirizzi syndrome and to analyze surgical outcomes depending on the effectiveness of minimally invasive biliary decompression.

Material And Methods: There were 67 patients with Mirizzi syndrome aged 27-96 years (mean age -64.8 years). The diagnosis was established on the basis of complaints, objective data, laboratory survey, abdominal X-ray, ultrasound (US), endoscopic gastroduodenoscopy (EGDS), computed tomography (CT) and magnetic resonance imaging (MRI). Extrahepatic bile duct visualization in case of suspected biliodigestive fistula was achieved by using of percutaneous transhepatic cholangiography, endoscopic retrograde cholangiopancreatography, cholecystocholangiography, intraoperative cholangiography.

Results: The analysis of the diagnosis and treatment of patients with Mirizzi syndrome and mechanical jaundice with and without symptoms of cholangitis was carried out. It should be noted that percutaneous transhepatic cholangiography and cholecystocholangiography with antegrade contrasting were able to confirm Mirizzi syndrome type 1 without complications. Retrograde cholangiopancreatography in patients with Mirizzi syndrome type 2 reduced the diagnostic value of contrast-enhancement with complications in every fifth patient. Percutaneous drainage for Mirizzi syndrome type 1 was effective in all patients. There was low effectiveness of medication for Mirizzi syndrome. Medication combined with antegrade biliary decompression was 7 times more effective than retrograde decompression. All patients underwent surgery. Mortality depended on surgical emergency and effectiveness of biliary decompression. So, emergency interventions were followed by mortality rate near 60% while there were no deaths after elective procedures. Overall mortality was 11.9%.
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http://dx.doi.org/10.17116/hirurgia201903142DOI Listing
May 2019

The Effect of Electromagnetic Fields of Extremely Low Frequency 30 Hz on Rat Ovaries.

Bull Exp Biol Med 2019 Mar 23;166(5):704-707. Epub 2019 Mar 23.

State Scientific Research Test Institute of Military Medicine, St. Petersburg, Russia.

We studied the effects of extremely low-frequency electromagnetic field on the ovaries in rats. The female rats were exposed to a 30 Hz sinusoidal magnetic field of 4 kA/m for 2 h per day for a period of 10 weeks, including the period of embryonic development. After the exposure, the duration of the estrous cycle and serum levels of luteinizing hormone, follicle stimulating hormone, progesterone, and estradiol E2 were measured in different phases of the estrous cycle and morphological changes in the ovaries were evaluated. Lower levels of follicle stimulating hormone in the proestrus and progesterone in the estrus phase were found; in the ovaries, the content of primordial follicles was lower than in the control. The exposure to electromagnetic field in the selected mode caused no significant defects in the structure and function of rat ovaries.
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http://dx.doi.org/10.1007/s10517-019-04422-2DOI Listing
March 2019

A case of a tumor-like condition in the optic nerve head of a pig.

Clin Case Rep 2018 Nov 9;6(11):2202-2207. Epub 2018 Oct 9.

State Scientific Research Test Institute of Military Medicine St. Petersburg Russia.

A tumor-like condition of the optic nerve head of unknown etiology was found in a domestic pig. Clinical and histological manifestations suggest that the unusual tumor-like condition is probably a variant of proliferative optic neuropathy caused by unknown nonspecific damage (perhaps trauma), which was received earlier.
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http://dx.doi.org/10.1002/ccr3.1858DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6230634PMC
November 2018