Publications by authors named "Rundong Liu"

4 Publications

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Resveratrol improved hippocampal neurogenesis following lead exposure in rats through activation of SIRT1 signaling.

Environ Toxicol 2021 May 12. Epub 2021 May 12.

Department of Environmental Health, College of Public Health, Zhengzhou University, Zhengzhou, China.

Lead (Pb) poses a potential environmental risk factor for cognitive dysfunction during early life and childhood. Resveratrol is considered a promising antioxidant with respect to the prevention of cognitive deficits and act as a potent SIRT1 agonist. Here in, this study aims to investigate the profile of neurogenesis markers following Pb exposure and to determine the regulatory role of resveratrol in this process. We confirmed firstly the protective effects of resveratrol against Pb-induced impairments of hippocampal neurogenesis in Male SD rats. Pb exposure early in life caused the altered expression of Ki-67, NeuN, caspase-3 and SIRT1signaling, thereby resulting in spatial cognitive impairment of adolescent rats. As expected, resveratrol reduced cognitive damage and promoted neurogenesis in Pb-induced injury by regulation of SIRT1 pathway. Collectively, our study establishes the efficacy of resveratrol as a neuroprotective agent and providesa strong rationale for further studies on SIRT1-mediated mechanisms of neuroprotective functions.
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http://dx.doi.org/10.1002/tox.23162DOI Listing
May 2021

Establishment of -Mediated Transformation of , a Model Lichen-Forming Fungus.

J Fungi (Basel) 2021 Mar 26;7(4). Epub 2021 Mar 26.

Korean Lichen Research Institute, Sunchon National University, Suncheon 57922, Korea.

Despite the fascinating biology of lichens, such as the symbiotic association of lichen-forming fungi (mycobiont) with their photosynthetic partners and their ability to grow in harsh habitats, lack of genetic tools manipulating mycobiont has hindered studies on genetic mechanisms underpinning lichen biology. Thus, we established an -mediated transformation (ATMT) system for genetic transformation of a mycobiont isolated from . A set of combinations of ATMT conditions, such as input biomass of mycobiont, co-cultivation period with cells, and incubation temperature, were tested to identify an optimized ATMT condition for the mycobiont. As a result, more than 10 days of co-cultivation period and at least 2 mg of input biomass of the mycobiont were recommended for an efficient ATMT, owing to extremely slow growth rate of mycobionts in general. Moreover, we examined T-DNA copy number variation in a total of 180 transformants and found that 88% of the transformants had a single copy T-DNA insertion. To identify precise T-DNA insertion sites that interrupt gene function in , we performed TAIL-PCR analyses for selected transformants. A hypothetical gene encoding ankyrin repeats at its C-terminus was interrupted by T-DNA insertion in a transformant producing dark-brown colored pigment. Although the identification of the pigment awaits further investigation, this proof-of-concept study demonstrated the feasibility of use of ATMT in construction of a random T-DNA insertion mutant library in mycobionts for studying genetic mechanisms behind the lichen symbiosis, stress tolerance, and secondary metabolite biosynthesis.
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http://dx.doi.org/10.3390/jof7040252DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065847PMC
March 2021

Highly Efficient Cleavage of Ether Bonds in Lignin Models by Transfer Hydrogenolysis over Dual-Functional Ruthenium/Montmorillonite.

ChemSusChem 2020 Sep 15;13(17):4579-4586. Epub 2020 Jun 15.

Department of Chemistry, Renmin University of China, Beijing, 100872, PR China.

Cleavage of ether bonds is a crucial but challenging step for lignin valorization. To efficiently realize this transformation, the development of robust catalysts or catalytic systems is required. In this study, montmorillonite (MMT)-supported Ru (denoted as Ru/MMT) is fabricated as a dual-functional heterogeneous catalyst to cleave various types of ether bonds through transfer hydrogenolysis without using any additional acids or bases. The prepared Ru/MMT material is found to efficiently catalyze the cleavage of various lignin models and lignin-derived phenols; cyclohexanes (fuels) and cyclohexanols (key intermediates) are the main products. The synergistic effect between electron-enriched Ru and the acidic sites on MMT contributes to the excellent performance of Ru/MMT. Systematic studies reveal that the reaction proceeds through two possible reaction pathways, including the direct cleavage of ether bonds and the formation of intermediates with one hydrogenated benzene ring, for all examined types of ether bonds, namely, 4-O-5, α-O-4, and β-O-4.
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http://dx.doi.org/10.1002/cssc.202000978DOI Listing
September 2020

Comparison of copper binding properties of DOM derived from fresh and pyrolyzed biomaterials: Insights from multi-spectroscopic investigation.

Sci Total Environ 2020 Jun 9;721:137827. Epub 2020 Mar 9.

State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China. Electronic address:

The binding of dissolved organic matter (DOM) with metals affects the latter's biogeochemical processing in the environment. This study used multi-spectroscopic analyses to compare the heterogeneities of the Cu(II) binding properties of DOM derived from fresh and pyrolyzed biomaterials. The results showed that the DOM derived from fresh macrophyte (MDOM) and their corresponding biochar (BDOM) consisted mostly of protein-like and humic-like substances, respectively. The stability constant (log K) of protein-like matter in the MDOM was 5.27, and the values of humic-like components in the BDOM were 4.32-5.15. Compared with the MDOM, the BDOM exhibited lower affinities and active binding sites for Cu(II). In addition, the BDOM contents decreased after pyrolysis. Therefore, the pyrolysis of fresh biomaterials into biochar is a promising method for reducing the potential migration risk posed by Cu(II) due to the MDOM being a positive carrier for Cu(II) contamination. Polysaccharide was the only functional group that participated in the binding of Cu(II) in both MDOM and BDOM. Aliphatic groups and amides associated with protein-like matter were responsible for the Cu(II) binding to MDOM, whereas phenolic and aromatic groups mainly participated in the complexation of BDOM-Cu(II). The CO group of amide I in the MDOM, and polysaccharide in the BDOM, showed the fastest response to Cu(II). This study was helpful for elucidating the effects of fresh and pyrolyzed biomaterials (biochars) on the environmental behavior of Cu(II) at the molecular level.
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http://dx.doi.org/10.1016/j.scitotenv.2020.137827DOI Listing
June 2020