Publications by authors named "Run Wang"

219 Publications

Clinical Evaluation of the Pre-Analytical Capabilities of Hemostasis Instrument.

Clin Appl Thromb Hemost 2022 Jan-Dec;28:10760296221118483

Department of Clinical Laboratory, 26447Peking university First Hospital, Beijing, China.

Evaluate the technical performance of the pre-analytical hemolysis-icterus-lipemia (HIL) check module on the ACL-TOP-750. 8433 routine coagulation samples were evaluated for HIL, the presence of clotting and low sample volume by both visual inspection and the pre-analytical HIL check module on the ACL-TOP-750. 7726 samples were in agreement with both methods and 707 were not consistent. 356 samples with low volume were identified by visual inspection and 920 by the instrument (2.7 mL threshold). Visual inspection identified 56 lipemic samples while 13 of those with moderate or high lipemia were identified by the instrument. Visual inspection identified 47 hemolyzed samples while 7 with moderate or high hemolysis were identified by the instrument. Both visual inspection and the instrument identified 36 icteric samples. For triglyceride concentration and bilirubin concentration, there was good correlation between the ACL-TOP-750 and the DXC800 biochemistry analyzer. Among 30 samples with varying amounts of clotting, 27 were discovered by visual inspection and 3 were discovered by the instrument. The pre-analytical check module on the ACL-TOP-750 improved the detection rate of samples below the target 2.7 mL volume, and the accuracy in detection of HIL. However, the automated method could not replace visual assessment of clotting in samples.
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http://dx.doi.org/10.1177/10760296221118483DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9358572PMC
August 2022

High-Resolution Ultrasound of the Forefoot and Common Pathologies.

Diagnostics (Basel) 2022 Jun 24;12(7). Epub 2022 Jun 24.

Department of Ultrasound, Peking University Third Hospital, Beijing 100191, China.

Forefoot pain is common in clinical practice. Careful history taking and routine physical examination are initially performed for diagnosis, but imaging can confirm the clinical suspicion and play a key role in management. Ultrasound (US) can provide a visualization of the fine anatomy of the forefoot and is a useful method for evaluating various lesions causing forefoot pain. In this review, we provide the detailed anatomical structures of the forefoot and their normal appearances on US. We also focus on the most common pathologies affecting the forefoot, including plantar plate tear, sesamoiditis, bone fracture, synovitis, tenosynovitis, bursitis, Morton's neuromas, and foreign bodies.
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http://dx.doi.org/10.3390/diagnostics12071541DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9322853PMC
June 2022

Cardioprotective Effect of Paeonol on Chronic Heart Failure Induced by Doxorubicin Regulating the miR-21-5p/S-Phase Kinase-Associated Protein 2 Axis.

Front Cardiovasc Med 2022 5;9:695004. Epub 2022 Jul 5.

Division of Cardiology, Department of Medicine, The University of Hong Kong, Shenzhen Hospital, Shenzhen, China.

Background: This study primarily explored the role of paeonol in doxorubicin (DOX)-induced chronic heart failure (CHF), considering the cardioprotective effect of paeonol on an epirubicin-induced cardiac injury.

Methods: DOX-induced CHF-modeled rats were treated with paeonol. Cardiac function and myocardial damage in rats were evaluated by using the multifunction instrument, and the histopathology, apoptosis, and the expression of miR-21-5p and S-phase kinase-associated protein 2 (SKP2) in myocardium were detected. The target gene of miR-21-5p was confirmed by a dual-luciferase reporter assay. After the required transfection or paeonol treatment, the viability, apoptosis, mitochondrial membrane potential (MMP), and reactive oxygen species (ROS) of the DOX-induced cardiomyocytes were determined. Reverse-transcription quantitative-PCR (RT-qPCR) and Western blot were performed to quantify the expressions of miR-21-5p, SKP2, and apoptosis-related factors.

Results: Paeonol improved cardiac function and also ameliorated the cardiac damage of CHF-modeled rats, where the downregulation of abnormally elevated myocardial damage markers, including brain natriuretic peptide, lactate dehydrogenase, renin, angiotensin II, aldosterone, and endothelin 1, was observed. Paeonol alleviated the histopathological injury and suppressed the apoptosis in CHF-modeled rats, inhibited miR-21-5p expression, and upregulated SKP2 expression and . miR-21-5p targeted SKP2. Paeonol and SKP2 increased the viability and MMP, but reduced apoptosis and ROS in the DOX-induced cardiomyocytes. miR-21-5p exerted effects opposite to PAE and SKP2, and it downregulated the expression of Bcl-2 and mitochondrion-Cytochrome c (Cyt c) and upregulated the expression of Bax, C-caspase-3, and cytoplasm-Cyt c. miR-21-5p reversed the effects of paeonol, and its effects were further reversed by SKP2.

Conclusion: Paeonol shows a cardioprotective effect on DOX-induced CHF regulating the miR-21-5p/SKP2 axis.
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http://dx.doi.org/10.3389/fcvm.2022.695004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9294229PMC
July 2022

Risk Factors of Postoperative Meningitis in Lateral Ventricular Trigone Meningiomas: A Clinical Analysis of 64 Patients.

Front Surg 2022 25;9:916053. Epub 2022 May 25.

Department of Neurosurgery, The First Hospital of China Medical University, Shenyang, China.

Purpose: Patients with intraventricular tumors are more susceptible to postoperative meningitis (POM) than other intracranial tumors. In this study, we explored the risk factors of POM in lateral ventricular trigone meningiomas (LVTMs).

Methods: Clinical features of 64 patients with LVTMs were analyzed. Age, gender, body mass index, medical history, intraoperative blood loss (IBL), intraventricular drainage placement, surgical duration, tumor grade, postoperative tumor cavity hemorrhage, and tumor size were included in univariate and multivariate analyses of POM.

Results: Of the 64 patients, 14 patients (21.9%) received diagnosis of POM. The univariate analysis revealed IBL ≥400 mL (odds ratio [OR], 9.012; = 0.003), tumor size ≥50 cm (OR, 3.071; = 0.080), and surgical duration ≥5 h (OR, 2.970; = 0.085) were considered possible risk factors for POM ( < 0.10). Tumor size ( = 0.514) and surgical duration ( = 0.624) were significantly correlated with IBL (< 0.05). In the multivariate analysis, only IBL was found to be an independent risk factor for POM.

Conclusion: The IBL ≥400 mL is independently associated with the increased risk of POM in LVTM patients. Our results demonstrate the importance of controlling IBL for preventing POM, especially in large tumors and long surgeries.
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http://dx.doi.org/10.3389/fsurg.2022.916053DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9174679PMC
May 2022

Complication Rates in Patients Using Intracavernosal Injection Therapy for Erectile Dysfunction With or Without Concurrent Anticoagulant Use-A Single-Center, Retrospective Pilot Study.

Sex Med 2022 Aug 3;10(4):100535. Epub 2022 Jun 3.

Department of Surgery, Division of Urology, University of Texas McGovern Medical School, Houston, TX, USA; Department of Urology, MD Anderson Cancer Center, Houston, TX, USA. Electronic address:

Background: Intracavernosal injection therapy (ICI) is an effective intervention used to treat erectile dysfunction (ED). It has been proposed that caution should be exercised when prescribing ICI to patients currently taking anticoagulants (AC) due to the theoretical increased risk of bleeding, however, there is limited literature describing complication rates of actively anticoagulated patients utilizing ICI.

Aim: We sought to determine whether there was a difference in bleeding and other complications in a cohort of patients using ICI therapy with or without concurrent AC use.

Methods: We reviewed our institutional electronic health record and identified 168 patients who were seen in our clinic from January to August 2020 who had either currently or previously utilized ICI therapy for ED treatment. These patients were surveyed regarding their ICI therapy as well as given the erectile dysfunction inventory for treatment satisfaction questionnaire. Data from 85 patients was obtained; 43 concurrently using AC during ICI therapy and 42 with no AC use. Fisher's exact test for categorical variables and a 2-tailed t-test were used with P < .05 considered to be significant.

Outcome: Documented bleeding events (eg, bruising, hematoma), complications, and mean erectile dysfunction inventory for treatment satisfaction scores were compared between the 2 groups.

Results: There were more absolute bleeding complications in the AC group vs the no AC group, with 3 of 43 AC patients (7%, 95% confidence interval: 2.4-18.6) and 0/42 no AC patients (0%, 95% confidence interval: 0-8.4) experiencing some type of bleeding complication on ICI. However, there was no statistically significant difference found in overall or stratified documented bleeding events and complications between the 2 groups.

Clinical Implications: Patients with concurrent AC usage on ICI therapy reported a higher rate of absolute bleeding complications than our non-AC group.

Strengths And Limitations: The strength of this study is addressing question of safety of ICI therapy in patients with concurrent AC usage. Limitations include single-center retrospective study design and underpowered sample size limiting confidence with which conclusions from data should guide future patient counseling regarding ICI risks.

Conclusion: Findings from a single-center cohort of patients suggest that ICI therapy may be a safe and effective treatment modality for ED in patients with concurrent anticoagulant usage, however, given the higher rate of absolute bleeding events in our AC cohort, future assessment in a higher-powered study is warranted in determining a more accurate estimation of risk or propensity for bleeding complications in patients on AC using ICI therapy. Blum KA, Mehr JP, Green T, et al. Complication Rates in Patients Using Intracavernosal Injection Therapy for Erectile Dysfunction With or Without Concurrent Anticoagulant Use-A Single-Center, Retrospective Pilot Study. Sex Med 2022;10:100535.
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http://dx.doi.org/10.1016/j.esxm.2022.100535DOI Listing
August 2022

Coexistence of meningioma and other intracranial benign tumors in non-neurofibromatosis type 2 patients: A case report and review of literature.

World J Clin Cases 2022 May;10(13):4249-4263

Department of Neurosurgery, The First Hospital of China Medical University, Shenyang 110001, Liaoning Province, China.

Background: The coexistence of meningioma and other intracranial primary benign tumors is rare, especially in non-neurofibromatosis type 2, and there is limited guidance for the management of such patients. Here, we report a series of 5 patients with concomitant meningioma and other intracranial benign tumors, including subependymoma and pituitary adenoma.

Case Summary: Five non-neurofibromatosis type 2 patients with simultaneous occurrence of meningioma and other intracranial benign tumors were retrospectively reviewed. The patients had no history of previous irradiation. The clinical features, pre- and postoperative imaging, surgical procedure and pathological findings were extracted from electronic medical records. There were 4 female patients (80%) and 1 male patient (20%). The mean age was 42.8 years (range: 29-52 years). The coexisting tumors included subependymoma in 1 case (20%) and pituitary adenoma in 4 cases (80%). The most common clinical symptom was headache (3/5, 60%). Four patients (80%) underwent craniotomy. One patient (20%) underwent transsphenoidal surgery followed by transcranial operation. All tumor diagnoses were confirmed by histopathological examination. The mean follow-up was 38.8 mo (range: 23-96 mo), and all 5 patients were in a stable condition at the last follow-up.

Conclusion: The simultaneous occurrence of meningioma and other intracranial benign tumors is a rare clinical event. Histological examination is necessary for the accurate diagnosis. Neurosurgeons should select the appropriate surgical strategy according to the clinical features of each patient, which may provide a more favorable prognosis for individual patients.
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http://dx.doi.org/10.12998/wjcc.v10.i13.4249DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9131210PMC
May 2022

Association between urine microscopy and severe acute kidney injury in critically ill patients following non-cardiac surgery: a prospective cohort study.

Ann Palliat Med 2022 Jul 19;11(7):2327-2337. Epub 2022 May 19.

Critical Care Nephrology Research Center, Peking University First Hospital, Beijing, China; Department of Nephrology, Peking University First Hospital, Beijing, China.

Background: Acute kidney injury (AKI) is a common and adverse complication following non-cardiac surgery. Evidence have shown urine microscopy could help early detection, differentiating the causes and predicting the progression of AKI. However, little evidence is available on AKI after non-cardiac surgery. Thus, we investigated the association between urine microscopy and severe AKI in critically ill patients after non-cardiac surgery.

Methods: This was a single-center prospective cohort study. The primary outcome was severe AKI, defined as stage 2 or 3 according to maximal KDIGO criteria within 7 days following non-cardiac surgery. Urine microscopy immediately, 6 and 12 hours after surgical intensive care unit (SICU) admission were examined and graded by a urine microscopy score (UMS) based on the observed quantification of renal tubular cells and casts in the sediment. Then, multivariate Logistic regression models were used to analyze the associations between UMS and postoperative severe AKI.

Results: From May 20, 2019 to November 24, 2020, 661 patients were enrolled with 147 patients (22.2%) developing postoperative severe AKI. Multivariate Logistic regression model showed elevated UMS (≥1) 6 and 12 hours after SICU admission were independently associated with postoperative severe AKI (OR 2.200, 95% CI: 1.182-4.095, P=0.013 and OR 2.949, 95% CI: 1.657-5.248, P<0.001, respectively). Furthermore, higher UMS 6 hours after SICU admission demonstrated correlation with greater risk of severe AKI with OR 3.887 (95% CI: 1.430-10.563) for UMS ≥3 and OR 2.429 (95% CI: 1.237-4.770) for UMS =1-2. The specificity and sensitivity of UMS ≥1 for severe AKI was 93.8% (95% CI: 91.7-95.9%) and 15.6% (95% CI: 9.7-21.5%), respectively. While the negative and positive predictive value was 79.5% (95% CI: 76.3-82.7%) and 41.8% (95% CI: 28.8-54.8%), respectively. In addition, patients with higher UMS (≥3, 1-2 and 0) had significantly more postoperative complications and longer SICU stay; and they also showed a trend toward other adverse postoperative outcomes.

Conclusions: Early urine microscopy was independently associated with severe AKI in critically ill patients following non-cardiac surgery with higher UMS related to greater risk.

Trial Registration: ClinicalTrials.gov identifier: NCT03880110.
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http://dx.doi.org/10.21037/apm-21-3085DOI Listing
July 2022

Role of Cardiomyocyte-Derived Exosomal MicroRNA-146a-5p in Macrophage Polarization and Activation.

Dis Markers 2022 2;2022:2948578. Epub 2022 May 2.

Division of Cardiology, Department of Medicine, The University of Hong Kong Shenzhen Hospital, Shenzhen, China.

Myocardial infarction arises from an excessive or prolonged inflammatory response, leading to ventricular remodeling or impaired cardiac function. Macrophages exhibit different polarization types associated with inflammation both at steady state and after myocardial infarction. Exosomal miR-146a-5p has been identified as an important molecule in the cardiovascular field in recent years. However, the effect of cardiomyocyte-derived exosomal miR-146a-5p on macrophages has not yet been elucidated. Initially, we found that exosomes with low expression of miR-146a-5p derived from myocardial infarction tissues modulated macrophage polarization. To determine whether cardiomyocyte-derived exosomal miR-146a-5p mediated macrophage polarization, we treated macrophages with exosomes rich in miR-146a-5p collected from neonatal mouse cardiomyocytes. The effects of exosomal miR-146a-5p on macrophage polarization were measured using RT-qPCR, transwell assays, and western blotting. The results showed that the increased expression of miR-146a-5p promoted M1 macrophage polarization, inhibited M2 macrophage polarization, and increased the expression of VEGFA. However, the decreased expression of exosomalmiR-146a-5p showed the opposite trends. Interestingly, in contrast to treatment with the solitary miR-146a-5p mimic, exosomal miR-146a-5p derived from neonatal mouse cardiomyocytes reduced TNF and iNOS expression. In addition, when macrophages were activated by the miR-146a-5p mimic or exosomal miR-146a-5p, the expression of TNF receptor-associated factor 6 (TRAF6), a target gene of miR-146a-5p, was reduced significantly. Taken together, these findings indicate that exosomal miR-146a-5p derived from cardiomyocytes could stimulate M1 macrophage polarization to induce an inflammatory reaction, while targeting TRAF6, exerting an anti-inflammatory effect. Exosomal miR-146a-5p plays important roles in macrophages, illuminating a novel potential therapeutic target in myocardial infarction.
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http://dx.doi.org/10.1155/2022/2948578DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9085364PMC
May 2022

Development and Validation of a Prognostic Model to Predict High-Risk Patients for Coronary Heart Disease in Snorers With Uncontrolled Hypertension.

Front Cardiovasc Med 2022 21;9:777946. Epub 2022 Apr 21.

Hypertension Center of People's Hospital of Xinjiang Uygur Autonomous Region, Ürümqi, China.

Purpose: Snoring or obstructive sleep apnea, with or without uncontrolled hypertension, is common and significantly increases the risk of coronary heart disease (CHD). The aim of this study was to develop and validate a prognostic model to predict and identify high-risk patients for CHD among snorers with uncontrolled hypertension.

Methods: Records from 1,822 snorers with uncontrolled hypertension were randomly divided into a training set ( = 1,275, 70%) and validation set ( = 547, 30%). Predictors for CHD were extracted to construct a nomogram model based on multivariate Cox regression analysis. We performed a single-split verification and 1,000 bootstraps resampling internal validation to assess the discrimination and consistency of the prediction model using area under the receiver operating characteristic curve (AUC) and calibration plots. Based on the linear predictors, a risk classifier for CHD could be set.

Results: Age, waist circumference (WC), and high- and low-density lipoprotein cholesterol (HDL-C and LDL-C) were extracted as the predictors to generate this nomogram model. The C-index was 0.720 (95% confidence interval 0.663-0.777) in the derivation cohort and 0.703 (0.630-0.776) in the validation cohort. The AUC was 0.757 (0.626-0.887), 0.739 (0.647-0.831), and 0.732 (0.665-0.799) in the training set and 0.689 (0.542-0.837), 0.701 (0.606-0.796), and 0.712 (0.615-0.808) in the validation set at 3, 5, and 8 years, respectively. The calibration plots showed acceptable consistency between the probability of CHD-free survival and the observed CHD-free survival in the training and validation sets. A total of more than 134 points in the nomogram can be used in the identification of high-risk patients for CHD among snorers with uncontrolled hypertension.

Conclusion: We developed a CHD risk prediction model in snorers with uncontrolled hypertension, which includes age, WC, HDL-C, and LDL-C, and can help clinicians with early and quick identification of patients with a high risk for CHD.
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http://dx.doi.org/10.3389/fcvm.2022.777946DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9069207PMC
April 2022

Countering Malicious DeepFakes: Survey, Battleground, and Horizon.

Int J Comput Vis 2022 4;130(7):1678-1734. Epub 2022 May 4.

Nanyang Technological University, Singapore, Singapore.

The creation or manipulation of facial appearance through deep generative approaches, known as , have achieved significant progress and promoted a wide range of benign and malicious applications, e.g., visual effect assistance in movie and misinformation generation by faking famous persons. The evil side of this new technique poses another popular study, i.e., aiming to identify the fake faces from the real ones. With the rapid development of the DeepFake-related studies in the community, both sides (i.e., DeepFake generation and detection) have formed the relationship of battleground, pushing the improvements of each other and inspiring new directions, e.g., the evasion of DeepFake detection. Nevertheless, the overview of such battleground and the new direction is unclear and neglected by recent surveys due to the rapid increase of related publications, limiting the in-depth understanding of the tendency and future works. To fill this gap, in this paper, we provide a comprehensive overview and detailed analysis of the research work on the topic of DeepFake generation, DeepFake detection as well as evasion of DeepFake detection, with more than 318 research papers carefully surveyed. We present the taxonomy of various DeepFake generation methods and the categorization of various DeepFake detection methods, and more importantly, we showcase the battleground between the two parties with detailed interactions between the adversaries (DeepFake generation) and the defenders (DeepFake detection). The battleground allows fresh perspective into the latest landscape of the DeepFake research and can provide valuable analysis towards the research challenges and opportunities as well as research trends and future directions. We also elaborately design interactive diagrams (http://www.xujuefei.com/dfsurvey) to allow researchers to explore their own interests on popular DeepFake generators or detectors.
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http://dx.doi.org/10.1007/s11263-022-01606-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9066404PMC
May 2022

Is There Still a Role for Vacuum Erection Devices in Contemporary Sexual Medicine?

Authors:
Run Wang

J Sex Med 2022 05 20;19(5):682-685. Epub 2022 Mar 20.

Director of Sexual Medicine Fellowship Program, University of Texas McGovern Medical School and MD Anderson Cancer Center, Houston, TX. Electronic address:

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http://dx.doi.org/10.1016/j.jsxm.2022.02.013DOI Listing
May 2022

Establishment of SYBR green I-based quantitative real-time polymerase chain reaction for the rapid detection of a novel Chaphamaparvovirus in cats.

3 Biotech 2022 Apr 14;12(4):91. Epub 2022 Mar 14.

Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036 People's Republic of China.

Feline parvovirus causes infectious diseases, and Chaphamaparvovirus is a novel type of feline parvovirus. The present study aims to establish a method that can be used in clinical rapid detection of feline Chaphamaparvovirus (FeChPV), for facilitate the timely and effective diagnosis and treatment of sick animals and shorten the diagnosis time of clinical diseases. The experimental samples in this study are from 20 cats undergoing physical examination in Hefei Xin'an Animal Hospital. An SYBR Green I-based qPCR assay was performed to detect FeChPV. A pair of specific primers was designed based on the gene to perform the assay. The detection assay showed high sensitivity with a detection limit of 1.07 × 10 copies/μL and high specificity for detection of only the target virus. The coefficients of value variation were calculated to assess the reproducibility of the qPCR assay, and the inter- and intra-assay ranged from 0.21 to 0.67% and 0.10 to 0.56%, respectively. The result of clinical sample detection showed that the infection rate of FeChPV in 124 samples detected using qPCR assay was higher than that with conventional PCR. The established qPCR assay could be a low-cost, convenient, and reliable method to detect FeChPV in clinical practice.
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http://dx.doi.org/10.1007/s13205-022-03150-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8918419PMC
April 2022

Improved Monitoring and Assessment of Meteorological Drought Based on Multi-Source Fused Precipitation Data.

Int J Environ Res Public Health 2022 01 29;19(3). Epub 2022 Jan 29.

Downstream Bureau of Changjiang Water Resources Commission, Wuhan 430062, China.

Meteorological drought, one of the most frequent climate-related disasters, causes great danger for human health and socioeconomic development. With an aim to improve the accuracy of meteorological drought monitoring, this study collected multi-source remotely-sensed precipitation products, i.e., the Tropical Rainfall Measuring Mission (TRMM), the Global Precipitation Measurement Mission (GPM), and Climate Hazards Group InfraRed Precipitation with Station data (CHIRPS), and compared their performance over Hubei Province, China. The geographic difference analysis was used to blend the best-fitted product with gauged precipitation data. Based on the fused dataset with verification, the spatio-temporal characteristics of drought were investigated. Results showed that GPM performed the best in precipitation numerical evaluation and event detection with a 5 mm/d threshold. The fused data accurately captured 80% of historical drought events and indicated that extreme annual droughts mainly occurred in the northern and northwestern regions, while slight, moderate, and severe droughts mainly occurred in the central and eastern parts. The short-term drought exhibited the highest frequency of 33% in summer and the lowest frequency of 27% in spring, while the medium-term drought showed a higher frequency in autumn and winter. This could be a preliminary assessment of drought based on multi-source fused precipitation data for precise drought outlook and risk management.
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http://dx.doi.org/10.3390/ijerph19031542DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8835182PMC
January 2022

Emerging Roles of Penile Traction Therapy and Vacuum Erectile Devices.

Sex Med Rev 2022 07 1;10(3):421-433. Epub 2022 Feb 1.

Department of Surgery, Division of Urology, University of Texas McGovern Medical School, Houston, TX, USA; Department of Urology, MD Anderson Cancer Center, Houston, TX, USA. Electronic address:

Introduction: Penile traction therapy (PTT) and vacuum erection devices (VED) are nonsurgical conservative treatment options that have been used in the treatment of various urologic and sexual disorders such as Peyronie's Disease (PD) and Erectile Dysfunction (ED). Recently expanded uses for these therapies now include penile lengthening and with surgical interventions such as penile prosthesis surgery (PPS) and radical prostatectomy (RP). These devices can be used as both monotherapy or combination therapy.

Objectives: To review the indications and clinical studies for PTT and VED.

Methods: A literature search was conducted using PubMed to identify relevant studies addressing PTT, VED, and their indications. Searched terms included penile traction therapy, penile traction device, vacuum erection device, Peyronie's disease, penile prosthesis, radical prostatectomy, subjectively small penis, penile lengthening, erectile dysfunction.

Results: PTT with dynamic traction devices has shown favorable benefits for PD in many studies. The benefits of VED for PD cannot be confirmed due to limited studies with poor quality. In posterior urethroplasty, VED shows promise postoperatively, with additional trials also needed. In PPS, both PTT and VED have had positive findings in pre- and postoperative treatment. In RP patients, VED use has had positive outcomes while new literature shows beneficial effects of dynamic PTT and provides a basis for future studies. VED use does not show great benefit in patients with small penis, however PTT does have some positive findings. In ED, VED has a history of successful use and PTT has promising new data available.

Conclusion: PTT and VED have been utilized in urologic and sexual conditions with various success. Several promising areas utilizing both PTT and VED are being studied, however, more research needs to be done in these areas prior to becoming a standard treatment. Mehr J, Santarelli S, Green TP, et al. Emerging Roles of Penile Traction Therapy and Vacuum Erectile Devices. Sex Med Rev 2022;10:414-426.
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http://dx.doi.org/10.1016/j.sxmr.2021.12.003DOI Listing
July 2022

Diabetic foot ulcer risk assessment and prevention in patients with diabetes: a best practice implementation project.

JBI Evid Implement 2022 Jan 10. Epub 2022 Jan 10.

School of Nursing Nanfang Hospital, Southern Medical University PR China Nanfang Nursing Centre for Evidence-based Practice: a Joanna Briggs Institute Centre of Excellence, Guangzhou, PR China.

Introduction And Aim: Diabetic foot ulcer (DFU) is one of the most serious complications in patients with diabetes. Early identification of risk factors can prevent its occurrence and delay its progression. The aim of this project is to conduct an audit of DFU risk assessment protocols at a large tertiary hospital and evaluate the impact of any changes in compliance with the developed evidence-based best practice criteria.

Methods: Preimplementation and postimplementation audits based on JBI's Practical Application of Clinical Evidence System were conducted at the Department of Endocrinology and Metabolism of a tertiary hospital in China. The Getting Research into Practice audit tool was used to analyze the barriers and inadequacies encountered in practice. A total of 12 nurses and 30 patients with diabetes were included in the baseline and follow-up audits.

Results: There were 15 baseline audits that indicated deficits in DFU risk assessment by nurses, with 0% compliance for three criteria and 50% compliance or less for seven others. Strategies developed by the project team to address the identified barriers to compliance were adopted, leading to significant improvement in compliance with most criteria at the follow-up audit compared with baseline with 11 criteria achieving at least 90% compliance.

Conclusion: The project showed that regular audits of foot ulcer risk assessment can help to identify barriers to their implementation. Advising patients of their risk status can support appropriate self-care practices. Further audits are needed to implement evidence-based practices in all aspects of diabetes patient care.
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http://dx.doi.org/10.1097/XEB.0000000000000306DOI Listing
January 2022

Clinical assessment and screening of stroke patients with aphasia: a best practices implementation project.

JBI Evid Implement 2021 Nov 11. Epub 2021 Nov 11.

Nanfang Hospital, Southern Medical University PR China Nanfang Nursing Centre for Evidence-Based Practice: A Joanna Briggs Institute Affiliated Group, Guangzhou, PR China.

Introduction And Aims: As a critical form of stroke damage, aphasia negatively impacts stroke patients' return to society. Speech and language intervention has been found to assist in optimizing poststroke aphasia patient outcomes; consequently, early identification and diagnosis are vital for poststroke aphasia to ensure that patients receive the rehabilitation they require. This project aimed to promote evidence-based practice (EBP) in the assessment and screening of stroke patients with aphasia and to improve the clinical outcomes of patients who suffer from poststroke aphasia in a large tertiary hospital.

Methods: The current evidence implementation project was conducted in the neurology and rehabilitation departments of a tertiary hospital in China. Six audit criteria were developed for the baseline and follow-up audits. The project used the Joanna Briggs Institute's (JBI) PACES software, as well as JBI's Getting Research into Practice audit and feedback tool, to foster evidence-based healthcare in practice.

Results: Although the performance of all evidence-based criteria during the baseline audit was poor, barriers were identified through baseline, and the project team carried out and implemented developed strategies following Getting Research into Practice resources. All the criteria improved from baseline after the follow-up cycle, with four out of six criteria achieving a compliance rate of 100%, and two evidence-based criteria recorded at 73 and 80% compliance, respectively.

Conclusion: The current project successfully increased EBP for the assessment and screening of stroke patients with aphasia. Further studies are needed to ensure the project's long-term sustainability.
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http://dx.doi.org/10.1097/XEB.0000000000000300DOI Listing
November 2021

Perovskite White Light Emitting Diodes: Progress, Challenges, and Opportunities.

ACS Nano 2021 Nov 10. Epub 2021 Nov 10.

MIIT Key Laboratory of Advanced Display Materials and Devices, School of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.

As global warming, energy shortages, and environment pollution have intensified, low-carbon and energy-saving lighting technology has attracted great attention worldwide. Light emitting diodes (LEDs) have been around for decades and are considered to be the most ideal lighting technology currently due to their high luminescence efficiency (LE) and long lifespan. Besides, along with the development of modern technology, lighting technologies with higher performance and more functions are desired. Perovskite based LEDs (PeLEDs) have recently emerged as ideal candidates for lighting technology owing to the extraordinary photoelectric properties of perovskite, such as high photoluminescence quantum yields (PLQYs), easy wavelength tuning, and low-cost synthesis. Herein, we open this review by introducing the background of white LEDs (WLEDs), including their light-emitting mechanism, typical characteristics, and key indicators in applications. Then, four main approaches to fabricate WLEDs are discussed and compared. After that, in accordance with the four categories, we focus on the recent progress of white PeLEDs (Pe-WLEDs), followed by the challenges and opportunities for Pe-WLEDs in practical application. Meanwhile, some pertinent countermeasures to their challenges are put forward. Finally, the development promise of Pe-WLEDs is explored.
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http://dx.doi.org/10.1021/acsnano.1c06849DOI Listing
November 2021

The Augmenta Implant: A Cadaver Model of a Novel Anatomic Subdermal Implant for Cosmetic Penile Enhancement.

Sex Med 2021 Dec 20;9(6):100447. Epub 2021 Oct 20.

Robert J Cornell MD PA, Houston, TX, USA.

Introduction: There are limited options for cosmetic penile enhancement and the correction of structural penile deformities.

Aim: We present the Augmenta, an innovative, technologically advanced subcutaneous elastomer-silicone cosmetic penile implant (CPI), as evaluated by objective structural changes in a cadaveric model.

Methods: Five fresh male cadaver pelves were utilized in this preclinical setting. Placement of a subcutaneous elastomer-silicone CPI was performed via a penoscrotal approach. Penile circumference was measured at the base and distal shaft before and after implant placement. Penile length was measured from the pubic bone to the tip of the glans penis before and after implantation of Augmenta.

Main Outcome Measure: Objective outcomes included changes in penile circumference and shaft length in both the flaccid and erect state, both before and after CPI placement.

Results: In 5 cadaver models, implantation of the Augmenta CPI increased flaccid penile shaft base circumference from an average of 8.7 cm to 12.0 cm after implantation (39.1% increase, P < .001). Flaccid distal circumference increased from 7.3 cm to 10.3 cm (41.8% increase, P = .020). During the erect state, penile base circumference increased from an average of 12.5 cm to 13.7 cm (6.3% increase, P = .273), while distal erect circumference changed from an average of 11.3 cm to 12.4 cm (9.7% increase, P = .298). Nonstretched flaccid shaft length increased from an average of 12.1 cm to 14 cm (15.2% increase, P = .073).

Clinical Implications: The Augmenta CPI could serve as alternative option for men seeking cosmetic penile enhancement.

Strengths And Limitations: Limitations include a small number of cadavers which do not completely reproduce living physiologic function and the use of a single technical approach. However, a consistent and standardized surgical approach reduces operator-dependent outcomes. Another strength is the use of fresh human cadavers that most closely represent live models.

Conclusion: In a cadaveric model, the Augmenta CPI demonstrates enhancements in penile girth in both the flaccid and erect state. The unique honeycomb technology, the embedded polyester mesh, and the antimicrobial hydrophilic coating of the Augmenta CPI may provide an advantage to currently available CPIs. Clinical trials will be needed to demonstrate both the safety and feasibility. Clavell-Hernández J, Tatem AJ, Lipshultz LI, et al. The Augmenta Implant: A Cadaver Model of a Novel Anatomic Subdermal Implant for Cosmetic Penile Enhancement. Sex Med 2021;9:100447.
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http://dx.doi.org/10.1016/j.esxm.2021.100447DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8766264PMC
December 2021

The Role of Long Term Label-Retaining Cells in the Treatment of Erectile Dysfunction by Vacuum Erectile Device.

Sex Med 2021 Dec 11;9(6):100442. Epub 2021 Oct 11.

Department of Surgery, The University of Texas Health Science Center at Houston, Houston, TX, USA; Department of Urology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA. Electronic address:

Introduction: Vacuum erectile device (VED) therapy is commonly used for penile rehabilitation after radical prostatectomy, however, the underlying mechanism of this effect is not fully understood.

Aim: To evaluate the presence of label-retaining cells (LRCs), cells with long-term retention of 5-ethynyl-2-deoxyuridine (EdU) labeling and recognized as adult stem cells or progenitor-like cells, in cavernous tissue after VED treatment using a BCNC rat model.

Methods: Postnatal pups (1 day old) of Sprague Dawley (SD) rats were intraperitoneally injected with EdU (50 ug/g, BID for 3 days) and BCNC surgery was conducted at 6 weeks old (designated as natal-labeled rats). Adult SD rats underwent BCNC surgery and EdU injection (50 ug/g, once) after surgery (designated as adult-labeled rats). One week after surgery, both natal- and adult-labeled rats received daily VED treatment for 4 weeks. Intracavernous pressure (ICP) and mean arterial pressure (MAP) were measured for all rats and then the penile tissue was harvested. The ratio of ICP/MAP was calculated to represent erectile function. Penile tissue was examined by immunofluorescence staining to detect EdU positive cells.

Main Outcome Measures: The ratio of Intracavernous pressure (ICP) /MAP and the percentage of EdU positive cells were measured.

Results: The erectile function was impaired after BCNC and partially restored after VED treatment in both natal- and adult-labeled rats (P < .05). There was no difference in the percentage of EdU positive cells in natal-labeled rat cavernous tissue in BCNC group compared with VED group. Among the adult-labeled rats, the percentage of EdU positive cells increased in BCNC group (P < .05) but didn't change significantly after VED treatment (P = .35).

Conclusion: LRCs may play a limited role in the restoration of erectile dysfunction through VED treatment after BCNC. Yang B, Luse D, Cao Y, et al. The Role of Long Term Label-Retaining Cells in the Treatment of Erectile Dysfunction by Vacuum Erectile Device. Sex Med 2021;9:100442.
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http://dx.doi.org/10.1016/j.esxm.2021.100442DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8766272PMC
December 2021

Decreased CD8 Lymphocytic Infiltration in Multifocal and Multicentric Glioblastomas.

Front Oncol 2021 27;11:748277. Epub 2021 Sep 27.

Department of Geriatrics, Shengjing Hospital of China Medical University, Shenyang, China.

Purpose: Multifocal and multicentric glioblastomas (mGBMs) are associated with a poorer prognosis compared to unifocal glioblastoma (uGBM). The presence of CD8 tumor-infiltrating lymphocytes (TILs) is predictive of clinical outcomes in human malignancies. Here, we examined the CD8 lymphocytic infiltration in mGBMs.

Methods: The clinical data of 57 consecutive IDH wildtype primary mGBM patients with histopathological diagnoses were retrospectively reviewed. CD8 TILs were quantitatively evaluated by immunohistochemical staining. The survival function of CD8 TILs was assessed by Kaplan-Meier analysis and Cox proportional hazard models.

Results: No significant difference in the concentration of CD8 TILs was observed among foci from the same patient (P>0.150). The presence of CD8 TILs was similar between multifocal and multicentric GBMs (P=0.885). The concentration of CD8 TILs was significantly lower in mGBMs than in uGBMs (P=0.002). In mGBM patients, the CD8 TIL level was associated with preoperative KPS (P=0.018). The median overall survival (OS) of the 57 mGBMs was 9 months. A low CD8 TIL level (multivariate HR 4.404, 95% CI 1.954-9.926, P=0.0004) was an independent predictor of poor OS, while postoperative temozolomide chemotherapy (multivariate HR 6.076, 95% CI 2.330-15.842, P=0.0002) was independently associated with prolonged OS in mGBMs.

Conclusions: Decreased CD8 TIL levels potentially correlate with unfavorable clinical outcome in mGBMs, suggesting an influence of the local immuno-microenvironment on the progression of mGBMs.
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http://dx.doi.org/10.3389/fonc.2021.748277DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8503598PMC
September 2021

Patient empowerment and self-management behaviour of chronic disease patients: A moderated mediation model of self-efficacy and health locus of control.

J Adv Nurs 2022 Apr 13;78(4):1055-1065. Epub 2021 Oct 13.

Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, PR China.

Aims: To explore the role of self-efficacy (SE) in the effect of patient empowerment on self-management behaviours among patients with chronic illness and to investigate the moderating effect of three types of health locus of control (HLC) in this moderated mediation model.

Design: Cross-sectional design.

Methods: Data were collected in a general tertiary hospital, and a sample of 254 patients was recruited between August and October 2020. The effect of moderation and mediation was tested by the PROCESS macro (Model 4 and Model 8) for SPSS 25.0 by Hayes using 5000 bootstrap samples.

Results: Self-efficacy significantly mediated the relationship between patient empowerment and self-management behaviour with a 95% confidence interval excluding zero. The chance HLC demonstrated a moderating effect, and the interaction effect on SE and self-management behaviour was significant.

Conclusion: Patient empowerment may improve confidence and adherence to self-management among people with chronic illness, and such benefits were conditional on the HLC of patients.

Impact: This study addresses the relationship between patient empowerment and self-management behaviour in patients with different personality characteristics. This result indicated that classifying the type of HLC may enable the identification of subgroups of patients who may subsequently benefit from patient empowerment. In a patient-centred programme, nurses and other healthcare professionals correctly identifying patients' HLC type and understanding the implications and then providing appropriate health care plans for patients with different health beliefs may be useful to tailor the decision-making process.
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http://dx.doi.org/10.1111/jan.15077DOI Listing
April 2022

Association between Plasma Homocysteine Concentrations and the First Ischemic Stroke in Hypertensive Patients with Obstructive Sleep Apnea: A 7-Year Retrospective Cohort Study from China.

Dis Markers 2021 28;2021:9953858. Epub 2021 Sep 28.

Hypertension Center of People's Hospital of Xinjiang Uygur Autonomous Region, Xinjiang Hypertension Institute, National Health Committee Key Laboratory of Hypertension Clinical Research, Xinjiang Clinical Medical Research Center for Hypertension Diseases, Urumqi, Xinjiang, China.

Purpose: This study was aimed at investigating the association between baseline plasma homocysteine (Hcy) concentrations and the risk of the first ischemic stroke (IS) and at investigating any possible influential modifying factors in hypertensive patients with obstructive sleep apnea (OSA).

Methods: Cox proportional hazards regression was employed to investigate the relationship between plasma Hcy concentration and the first IS. A generalized additive model was applied to determine the nonlinear relationship. In addition, we conducted subgroup analysis.

Results: A total of 2350 hypertensive patients with OSA without a history of IS were enrolled in this study. At a median follow-up of 7.15 years, we identified 93 cases of the first IS. After adjusting for potential confounding, the findings revealed that plasma Hcy concentration was strongly and positively associated with the occurrence of the first IS (per SD increment; HR = 1.37, 95% CI: 1.30-1.44). A nonlinear relationship was found between plasma Hcy concentration and the risk of developing the first IS with inflection points for plasma Hcy of 5 mol/L. In stratified analysis, a greater positive correlation was found between baseline plasma Hcy concentrations and new-onset IS in patients with DBP ≥ 90 mmHg (per SD increment; HR = 1.48, 95% CI: 1.33-1.65 vs. <90 mmHg: HR = 1.20, 95% CI: 1.02-1.42; -interaction = 0.04) and BMI ≥ 24 and <28 kg/m (per SD increment; HR = 1.46, 95% CI: 1.26-1.70 vs. <24 kg/m: HR = 1.13, 95% CI: 0.95-1.33 vs. ≥28 kg/m: HR = 1.46, 95% CI: 1.25-1.70; -interaction = 0.03).

Conclusion: Elevated plasma Hcy concentrations are independently associated with the risk of the first IS in hypertensive patients with OSA. Plasma Hcy concentrations ≥ 5 mol/L surely increased the risk of the first IS in hypertensive patients with OSA.
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http://dx.doi.org/10.1155/2021/9953858DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8492296PMC
January 2022

Research progress of full electroluminescent white light-emitting diodes based on a single emissive layer.

Light Sci Appl 2021 Oct 5;10(1):206. Epub 2021 Oct 5.

MIIT Key Laboratory of Advanced Display Materials and Devices, Institute of Optoelectronics & Nanomaterials, College of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing, 210094, China.

Carbon neutrality, energy savings, and lighting costs and quality have always led to urgent demand for lighting technology innovation. White light-emitting diodes (WLEDs) based on a single emissive layer (SEL) fabricated by the solution method have been continuously researched in recent years; they are advantageous because they have a low cost and are ultrathin and flexible. Here, we reviewed the history and development of SEL-WLEDs over recent years to provide inspiration and promote their progress in lighting applications. We first introduced the emitters and analysed the advantages of these emitters in creating SEL-WLEDs and then reviewed some cases that involve the above emitters, which were formed via vacuum thermal evaporation or solution processes. Some notable developments that deserve attention are highlighted in this review due to their potential use in SEL-WLEDs, such as perovskite materials. Finally, we looked at future development trends of SEL-WLEDs and proposed potential research directions.
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http://dx.doi.org/10.1038/s41377-021-00640-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8492743PMC
October 2021

Does knowing pre-operative penile length influence patient satisfaction post penile prosthesis implantation?

Int J Impot Res 2021 Sep 27. Epub 2021 Sep 27.

University of Texas Health Science Center at Houston, McGovern Medical School, Houston, TX, USA.

Patients who undergo penile prosthesis implantation as treatment for erectile dysfunction commonly complain of penile shortening after implantation. We conducted a study to determine whether knowledge of pre-operative stretched penile length measurement influences patient satisfaction. This prospective study consisted of 149 patients undergoing inflatable penile prosthesis (IPP) implantation from August 2017 to December 2019. Study group participants underwent pre-operative stretched penile length measurement in clinic while the control group did not. Six months post-operatively, patients completed a modified 14-item Erectile Dysfunction Inventory of Treatment Satisfaction (EDITS) questionnaire to assess overall satisfaction and penile length satisfaction. A total of 102 patients were eligible for final analysis (49 in study group, 53 in control). Mean scores for overall treatment satisfaction were 3.57 and 3.53 (scale from 0 to 4) in the study versus control group, respectively (p = 0.483). Mean scores for satisfaction with penile length were 4.08 and 4.11 (scale from 1 to 5) in the study vs. control group (p = 0.645). The study suggests that knowledge of pre-operative stretched penile length does not influence post-operative satisfaction after penile prosthesis implantation. Therefore, performing pre-operative measurements in clinic solely for informing the patient may be unnecessary. Current interventions aimed at conserving penile length may be effective at maintaining satisfaction with penile length.Trial Registration- This trial is registered and approved by the IRB committee at our institution, ID: HSC-MS-19-0320.
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http://dx.doi.org/10.1038/s41443-021-00472-1DOI Listing
September 2021

The Diagnostic Role of Neurophysiological Tests for Premature Ejaculation: A Prospective Multicenter Study.

J Urol 2022 01 30;207(1):172-182. Epub 2021 Aug 30.

Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Purpose: Premature ejaculation (PE) is one of the most common male sexual dysfunctions. Local anesthetics (LAs) and dapoxetine are frequently used to treat PE; however, previous studies show variable efficacy. This study aims to determine the efficacy of LAs and dapoxetine using a novel classification based on neurophysiological tests.

Materials And Methods: This multicenter cohort study enrolled adult men (568) with an intravaginal ejaculatory latency time (IELT) ≤2 minutes. Patients were divided into 4 groups according to the results of neurophysiological tests and assigned different treatments for 12 weeks: 1) penile sensory hyperexcitability type (Sens)-LAs; 2) penile sympathetic hyperexcitability type (Symp)-dapoxetine; 3) mixed type (Mixed)-both LAs and dapoxetine; 4) normal type (Norm)-both LAs and dapoxetine. Self-estimated IELT and patient-reported outcomes were recorded.

Results: The total percentage of men achieving IELT >2 minutes and ≥5 minutes after treatment were 82.7% and 76.7%, respectively. For men with abnormal results of neurophysiological tests, 401 (86.6%) had improved IELT >2 minutes after the 12-week treatment course, in which 375 (81.0%) achieved IELT ≥5 minutes. All patient-reported outcome measures improved in each group after 12 weeks of treatment, with greater improvements among those with abnormal neurophysiological tests.

Conclusions: The efficacy of LAs and dapoxetine increased in PE patients with abnormal results of neurophysiological tests. This novel classification of PE using neurophysiological tests could help guide and improve efficacy of PE therapies.
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http://dx.doi.org/10.1097/JU.0000000000002198DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8667790PMC
January 2022

Intracellular inhibits autophagy of bovine mammary epithelial cells through activating p38α.

J Dairy Res 2021 Aug;88(3):293-301

College of Veterinary Medicine, China Agricultural University, Beijing, China.

Staphylococcus aureus is a common pathogen of bovine mastitis which can induce autophagy and inhibit autophagy flux, resulting in intracellular survival and persistent infection. The aim of the current study was to investigate the role of p38α in the autophagy induced by intracellular S. aureus in bovine mammary epithelial cells. An intracellular infection model of MAC-T cells was constructed, and activation of p38α was examined after S. aureus invasion. Through activating/inhibiting p38α by anisomycin/SB203580, the autophagosomes, LC3 and p62 level were analyzed by immunofluorescence and western blot. To further study the detailed mechanism of p38α, phosphorylation of ULK1ser757 was also detected. The results showed that intracellular S. aureus activated p38α, and the activation developed in a time-dependent manner. Inhibition of p38α promoted intracellular S. aureus-induced autophagy flow, up-regulated the ratio of LC3 II/I, reduced the level of p62 and inhibited the phosphorylation of ULK1ser757, whereas the above results were reversed after activation of p38α. The current study indicated that intracellular S. aureus can inhibit autophagy flow by activating p38α in bovine mammary epithelial cells.
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http://dx.doi.org/10.1017/S0022029921000649DOI Listing
August 2021

Catalytic System-Controlled Divergent Reaction Strategies for the Construction of Diversified Spiropyrazolone Skeletons from Pyrazolidinones and Diazopyrazolones.

Angew Chem Int Ed Engl 2021 09 18;60(39):21327-21333. Epub 2021 Aug 18.

State Key Laboratory of Natural Medicines and Department of Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing, Jiangsu, 210009, China.

A catalytic system-controlled divergent reaction strategy was here reported to construct four types of intriguing spiroheterocyclic skeletons from simple and readily available starting materials via a precise chemical bond activation/[n+1] annulation cascade. The tetraazaspiroheterocyclic and trizazspiroheterocyclic scaffolds could be independently constructed by a selective N-N bond activation/[n+1] annulation cascade, a C(sp )-H activation/[4+1] annulation and a novel tandem C(sp )-H/C(sp )-H bond activation/[4+1] annulation strategy, along with a broad scope of substrates, moderate to excellent yields and valuable transformations. More importantly, in these transformations, we are the first time to capture a N-N bond activation and a C(sp )-H bond activation of pyrazolidinones under different catalytic system.
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http://dx.doi.org/10.1002/anie.202105857DOI Listing
September 2021

MCM8 is regulated by EGFR signaling and promotes the growth of glioma stem cells through its interaction with DNA-replication-initiating factors.

Oncogene 2021 07 15;40(27):4615-4624. Epub 2021 Jun 15.

Department of Neurosurgery, The First Hospital of China Medical University, Shenyang, China.

Mini-chromosome maintenance (MCM) proteins are critical components of DNA-replication-licensing factors. MCM8 is an MCM protein that exhibits oncogenic functions in several human malignancies. However, the role of MCM8 in glioblastomas (GBMs) has remained unclear. In the present study, we investigated the biological functions and mechanisms of MCM8 in glioma stem cells (GSCs). The clinical relevance of MCM8 mRNA expression was explored via TCGA and REMBRANDT datasets. The effects of MCM8 on the self-renewal and tumorigenicity of GSCs were examined both in vitro and in vivo. The regulation of MCM8 expression and its interacting proteins were also evaluated. We found that the expression of MCM8 was elevated in high-grade gliomas and classical molecular subtypes and was inversely correlated with patient prognosis. GSCs had a significantly higher expression of MCM8 compared with that in normal glioma cells. Silencing of MCM8 induced G0/G1 arrest and apoptosis, as well as inhibited the proliferation and self-renewal of GSCs. Forced expression of MCM8 enhanced clonogenicity of GSCs both in vitro and in vivo. MCM8 expression was regulated by EGFR signaling, which was mediated by NF-κB (p65). MCM8 interacted with DNA-replication-initiating factors-including EZH2, CDC6, and CDCA2-and influenced these factors to associate with chromatin. In addition, MCM8 knockdown increased the sensitivity of GSCs to radiation and TMZ treatments. Our findings suggest that MCM8, regulated by the EGFR pathway, maintains the clonogenic and tumorigenic potential of GSCs through interaction with DNA-replication-initiating factors; hence, MCM8 may represent a novel therapeutic target in GBMs.
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http://dx.doi.org/10.1038/s41388-021-01888-1DOI Listing
July 2021

Spherical-shaped CuS modified carbon nitride nanosheet for efficient capture of elemental mercury from flue gas at low temperature.

J Hazard Mater 2021 08 20;415:125692. Epub 2021 Mar 20.

School of Hydraulic Energy and Power Engineering, Yangzhou University, Yangzhou 225127, China.

Mercury (Hg) pollution poses a huge threat to human health and the environment due to its high toxicity, long persistence and bioaccumulation in the environment. Most of the traditional Hg adsorbents have a low reaction rate, high operating cost, especially poor resistance to SO, which limited their practical application. In this work, nanosheet g-CN was used as the support and modified by CuS to capture flue gas mercury. Take advantage of the large specific surface area of g-CN to increase the BET of the composite and decrease the use of CuS. The effects of CuS loading, reaction temperature, and common components in the coal-fired flue gas on the mercury removal performance were studied respectively. The experimental outcomes showed that the 10CuS/g-CN (10CuS/CN) reaches as high as almost 100% Hg removal efficiency under the temperature of 40-120 ℃. Meanwhile the common components like SO, NO, HCl and HO have no obvious inhibition effects on Hg removal efficiency of the 10CuS/CN adsorbent. S and Cu as the primary bonding sites shows a synergy effect on Hg removal. 10CuS/CN is a promising material for Hg removal under various flue gas conditions, which is expected to be a substitute for traditional adsorbents.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125692DOI Listing
August 2021

Erosion of Inflatable Penile Prosthesis with Prolonged Foley Catheterization in the COVID-19 Era.

Sex Med 2021 Jun 30;9(3):100371. Epub 2021 May 30.

Department of Urology, UT-Houston Medical School, Houston, TX, USA.

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http://dx.doi.org/10.1016/j.esxm.2021.100371DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164880PMC
June 2021
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