Publications by authors named "Ruixue Zhang"

114 Publications

Divergent Synthesis of Fused Tetracyclic Heterocycles from Diarylalkynes Enabled by the Selective Insertion of Isocyanide.

Angew Chem Int Ed Engl 2022 Jul 22. Epub 2022 Jul 22.

South China University of Technology, School of Chemistry & Chemical Engineering, No. 381, Wushan Road, Tianhe Strict,, 510640, Guangzhou, CHINA.

Herein, we present a direct and efficient synthesis of diverse polysubstituted fused tetracyclic heterocycles with good functional group tolerance from diarylalkynes under different palladium catalytic systems. In this chemistry, an unprecedented intermolecular nucleopalladation of diarylalkynes through the highly selective sequential double insertion of isocyanide was achieved for the first time. The practicality of this method was further demonstrated by the construction of various bioactive molecules and important structural motifs, with potential applications in materials science and biochemistry. In addition, density functional theory calculations (DFT) elucidated an interesting "Pd walk" might occur during the cyclization process.
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http://dx.doi.org/10.1002/anie.202208203DOI Listing
July 2022

Calcium/calmodulin-dependent protein kinase IV promotes imiquimod-induced psoriatic inflammation via macrophages and keratinocytes in mice.

Nat Commun 2022 Jul 22;13(1):4255. Epub 2022 Jul 22.

Department of Dermatology, the First Affiliated Hospital of Anhui Medical University, Hefei, China.

CaMK4 has an important function in autoimmune diseases, and the contribution of CaMK4 in psoriasis remains obscure. Here, we show that CaMK4 expression is significantly increased in psoriatic lesional skin from psoriasis patients compared to healthy human skin as well as inflamed skin from an imiquimod (IMQ)-induced mouse model of psoriasis compared to healthy mouse skin. Camk4-deficient (Camk4) mice treated with IMQ exhibit reduced severity of psoriasis compared to wild-type (WT) mice. There are more macrophages and fewer IL-17Aγδ TCR cells in the skin of IMQ-treated Camk4 mice compared to IMQ-treated WT mice. CaMK4 inhibits IL-10 production by macrophages, thus allowing excessive psoriatic inflammation. Deletion of Camk4 in macrophages alleviates IMQ-induced psoriatic inflammation in mice. In keratinocytes, CaMK4 inhibits apoptosis as well as promotes cell proliferation and the expression of pro-inflammatory genes such as S100A8 and CAMP. Taken together, these data indicate that CaMK4 regulates IMQ-induced psoriasis by sustaining inflammation and provides a potential target for psoriasis treatment.
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http://dx.doi.org/10.1038/s41467-022-31935-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307837PMC
July 2022

Rare mutations in NLRP3 and NLRP12 associated with familial cold autoinflammatory syndrome: two Chinese pedigrees.

Clin Rheumatol 2022 Jul 19. Epub 2022 Jul 19.

Department of Dermatology, The First Affiliated Hospital of Anhui Medical University, #69 Meishan Road, Hefei, 230022, Anhui, China.

Familial cold autoinflammatory syndrome (FCAS) is the mildest subtype of cryopyrin-associated periodic syndrome (CAPS) and is a rare inherited systemic autoinflammatory disease (SAID). CAPS is the consequence of a rare group of genetic disorders that are mostly reported in European and American populations, but scarcely reported in Chinese populations. NLRP3, NLRP12, PLCG2, and NLRC4 are known pathogenic genes associated with FCAS, and the aim of this study was to identify pathogenic mutations in two intact pedigrees of Chinese FCAS. We performed Sanger sequencing of genomic DNA samples from 25 affected and 32 unaffected members of the two intact pedigrees and analyzed the pathogenic mutations for their conservativeness using multiple sequence alignment tools. In addition, we reviewed previously reported pathogenic genes of FCAS and their pathogenicity classification and summarized the characteristics of different genotypes and phenotypes of FCAS. This study reported two intact FCAS pedigrees with different genotypes and phenotypes, the heterozygous mutation (p.V72M) in NLRP3 in pedigree 1 and the heterozygous mutation (p.R754H) in NLRP12 in pedigree 2. There are no reports targeting p.V72M in NLRP3 in FCAS1, and there are relatively few relevant phenotypic data on the clinical manifestations identified in previous pedigrees. Multiple sequence comparisons of NLRP3 indicate that the p.V72M mutation is highly conserved during evolution. Our study has enriched the understanding of the pathogenesis of FCAS, a rare disease especially in Asian populations. KEY POINTS: •The NLRP3 (p.V72M) variant was first discovered in the Chinese pedigree of FCAS1 •NLRP12 (p.R754H) variants are not conserved in multiple sequence alignments, but they are still co-segregated and expressed in the big Chinese diseased pedigree.
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http://dx.doi.org/10.1007/s10067-022-06292-yDOI Listing
July 2022

Human Leukocyte Antigen Fine-Mapping and Correlation Analysis of Han and Minority Leprosy Patients in Southern China.

Front Genet 2022 13;13:888361. Epub 2022 Jun 13.

Department of Dermatology, No. 1 Hospital, Anhui Medical University, Hefei, China.

Leprosy is very prevalent in many populations around the world, which is well known that both alleles for human leukocyte antigen (HLA) as well as single nucleotide polymorphisms (SNPs) in the HLA region are common in leprosy patients. Previous studies have identified leprosy-associated susceptibility genes that explain only part of disease risk and heritability. In view of the complicated characteristics of the major histocompatibility complex (MHC) region, this study aimed to explore the development and variation of HLA in leprosy and its possible mechanism. Previous genome-wide association data were extracted from Han and minority populations in southern China for HLA fine-mapping studies. Insertion and deletion (INDEL), SNP, and copy number variation (CNV) imputation were determined by using the Thousand People Database (1KGP Phase 3 Dataset) as a reference panel. The HAN-MHC database was used to input the HLA classical alleles and amino acids in the MHC region, and further step-regression analysis was performed to analyze independent variation signals associated with leprosy. The most significant locus rs75324027 (the same locus as rs602875 in the HLA-DR region) [ = 7.49E-09, OR= 0.62, 95%,CI: 0.52-0.73] in the intergene region between HLA-DQA1 and HLA-DRB1 was related with leprosy in M-S(Han leprosy patients in south China)disease. In M-SM (Leprosy patients of ethnic minorities in south China)disease, one of the most significant loci of the HLA-DQB1 gene was 6-32626438-A-T ( = 4.49E-08, OR = 0.36, 95%,CI: 0.25-0.52). Therefore, rs75324027 is a locus in M-S disease, and 6-32626438-a-T may be a new locus in M-SM disease. The interaction between 6 and 32626438-A-T and RS75324027 was analyzed, and A significant interaction relationship was found. In the optimal model, the accuracy of prediction was 0.5974, cross-validation Consistency:10, = 0.0107. In conclusion, this study is the first to assess the association between HLA and leprosy susceptibility in Han and other minority populations in southern China using the Thousand Population database and the Han MHC database. In addition, our analysis validated the previously reported locus rs602875 in the HLA-DR region and for the first time identified an unreported independent locus in leprosy among ethnic minorities in southern China.
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http://dx.doi.org/10.3389/fgene.2022.888361DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9234480PMC
June 2022

Imputation of the major histocompatibility complex region identifies major independent variants associated with bullous pemphigoid and dermatomyositis in Han Chinese.

J Dermatol 2022 Jun 25. Epub 2022 Jun 25.

Department of Dermatology, The First Affiliated Hospital of Anhui Medical University, Hefei, China.

As autoimmune skin diseases, both bullous pemphigoid (BP) and dermatomyositis (DM) show significant associations with the major histocompatibility complex (MHC) region. In fact, the coexistence of BP and DM has been previously reported. Therefore, we hypothesized that there may be a potential genetic correlation between BP and DM. Based on data for 312 BP patients, 128 DM patients, and 6793 healthy control subjects, in the MHC region, we imputed single-nucleotide polymorphisms (SNP), insertions and deletions (INDEL), and copy number variations (CNV) using the 1KGP phase 3 dataset and amino acids (AA) and SNP using a Han-MHC reference database. An association study revealed the most significant SNP associated with BP, namely, rs580921 (p = 1.06E-08, odds ratio [OR] = 1.61, 95% confidence interval [CI] = 1.37-1.90), which is located in the C6orf10 gene, and the most significant classic human leukocyte antigen (HLA) allele associated with DM, namely, HLA-DPB1*1701 (p = 6.56E-10, OR = 3.61, 95% CI = 2.40-5.42). Further stepwise regression analyses with rs580921 identified a threonine at position 163 of the HLA-B gene as a new independent disease-associated AA, and HLA-DPB1*1701 indicated that no loci were significant. Three-dimensional ribbon models revealed that the HLA-B AA position 163 (p = 3.93E-07, OR = 1.64, 95% CI = 1.35-1.98) located in the α2 domain of the HLA-B molecule was involved in the process of specific antigen presentation. The calculations showed that there was no significant genetic correlation between BP and DM. Our study identified three significant loci in the MHC region, proving that the HLA region was significantly correlated with BP and DM separately. Our research highlights the key role of the MHC region in disease susceptibility.
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http://dx.doi.org/10.1111/1346-8138.16499DOI Listing
June 2022

Mitochondrial-Targeted Antioxidant Peptide SS31 Prevents RPE Cell Death under Oxidative Stress.

Biomed Res Int 2022 27;2022:6180349. Epub 2022 May 27.

Department of Ophthalmology, The Second Affiliated Hospital of Xi'an Medical University, Xi'an, China.

This work aims at investigating the protective effects of the mitochondria-targeted peptide SS31, on mitochondria function, preventing human retinal pigment epithelial cell-19 (ARPE-19) cell apoptosis. The ARPE-19 cells were subjected to 24 h of intervention with HO of various concentrations (0, 100, 150, 200, 250, 300, and 500 mol/L). Various concentrations of SS31 (10 nM, 100 nM, and 1 mol/L) pretreated the cells for 2 h. The MTT assay determined cell viability. ARPE-19 cell apoptosis was observed by 4',6-diamidino-2-phenylindole (DAPI) staining under fluorescence microscope and detected by Annexin-V/PI staining under flow cytometry. The measurement of reactive oxygen species (ROS) release level used MitoSOX Red (a mitochondrial superoxide indicator) and the probe 2'-7'dichlorofluorescin diacetate (DCFH-DA). And with the use of a JC-1 probe, the mitochondrial membrane potential (MMP; ΔΨ) was analyzed. Reverse transcription polymerase chain reaction (RT-PCR) and real-time PCR were responsible for measuring the levels of apoptosis related genes (Bcl-2, Bax, and Caspase-3). The cell viability increased significantly with SS31 pretreated ( < 0.05). In the SS31 + HO group, the fluorescence of the cell nuclei with DAPI staining was weaker than HO along group accordance with the decreased ratio of apoptotic cells ( < 0.05). The ROS generation decreased in SS31 pretreated group, with the increased ΔΨ. The RT-PCR result showed decreased Bax gene and Caspase-3 gene expression with SS31 pretreatment, while increased antiapoptotic gene Bcl-2 ( < 0.05). We provide evidence that SS31 promotes resilience of RPE cells to oxidative stress by stabilizing mitochondrial function.
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http://dx.doi.org/10.1155/2022/6180349DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9167025PMC
June 2022

Modified Trabeculectomy versus Glaucoma Drainage Implant Surgery: A Retrospective Comparative Study for Refractory Glaucoma Treatment.

Oxid Med Cell Longev 2022 23;2022:3050007. Epub 2022 May 23.

Department of Ophthalmology, Xianyang First People's Hospital, Baoji, 712099 Shaanxi Province, China.

Purpose: To observe and compare the efficacy of modified trabeculectomy (TE), Ahmed drainage valve implantation (AGV), and EX-PRESS glaucoma shunt for refractory glaucoma (RG).

Methods: The study population of this retrospective study comprised 73 patients (76 eyes) who were suffering from RG and treated with modified TE, AGV, and EX-PRESS glaucoma shunt in our hospital from October 2012 to October 2020. The number of cases who underwent modified TE, AVG, and EX-PRESS glaucoma shunt was 36 (38 eyes). 19 (20 eyes), and 18 patients (18 eyes), respectively. The intraocular pressure (IOP), best-corrected visual acuity (BCVA), postoperative antiglaucoma medications, filter bubble morphology, anterior chamber depth (ACD), successful rate, and postoperative complications were recorded and statistically analyzed preoperative and 1 d, 1 w, 1 mon, 3 mon, 6 mon, and the end follow-up after operation.

Results: The BCVA differed insignificantly among the three cohorts before and 6 months after surgery. Compared to preoperative BCVA, the postoperative BCVA of the three groups had no statistical significance. An obvious reduction in IOP was observed in all the three group after operation ( < 0.05). An obvious decrease in antiglaucoma medications was observed after surgery in all the three groups ( < 0.05). The AGV group showed deeper ACD postoperatively, while no marked difference was found in postoperative ACD in the other two groups. The total success rates in modified TE and AGV groups were slightly higher than those in the EX-PRESS group. The three groups differed insignificantly in filter bubble morphology after operation.

Conclusion: Modified TE, AGV, and EX-PRESS glaucoma shunt showed equivalent efficacy for RG, which could validly reduce IOP and postoperative antiglaucoma medications. However, the success rates of modified TE and AGV were slightly higher than those of EX-PRESS glaucoma shunt in the last follow-up, and their complications were slightly less than those of the EX-PRESS glaucoma shunt.
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http://dx.doi.org/10.1155/2022/3050007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9150991PMC
June 2022

Perioperative Management and Long-Term Outcomes in Ocular Cicatricial Pemphigoid Patients Undergoing Cataract Surgery.

Oxid Med Cell Longev 2022 30;2022:2496649. Epub 2022 Apr 30.

Department of Ophthalmology, Peking University Third Hospital, Beijing Key Laboratory of Restoration of Damaged Ocular Nerve, Peking University Third Hospital, Beijing 100191, China.

Objective: To observe the outcomes of cataract surgery in ocular cicatricial pemphigoid (OCP) patients and explore routine perioperative medical treatments.

Design: Retrospective case series.

Methods: Fourteen eyes of 8 patients were included in the study. Foster's stage 1-4 OCP patients were given human intravenous immunoglobulin, whereas patients with active inflammation were treated with prednisone tablets and methotrexate. Those who were intolerant to methotrexate and had severe inflammatory symptoms were treated with cyclophosphamide. Cataract surgery was performed for all patients after three months of systemic treatment under stable conditions. The conjunctival biopsy was evaluated by immunofluorescence microscopy. Then, patients were divided into individuals with or without ankyloblepharon. Records were reviewed for OCP stage, type of surgery, best-corrected visual acuity (BCVA), Schirmer I test, corneal fluorescein sodium staining, meibomian gland coloboma range, and ocular surface disease index (OSDI) scores. Follow-up was for the duration of taking topical and systemic medication.

Results: Nine female (64.29%) and 4 male (35.71%) eyes were diagnosed with OCP by biopsy. The mean follow-up time was 60.64 ± 35.62 months. Thirteen eyes (92.86%) of 7 patients underwent phacoemulsification. One eye underwent phacoemulsification combined with amniotic membrane transplantation. The intracapsular extraction of cataract was applied to one eye. The BCVA improved significantly in all the patients, which remained stable until the last follow-up. The Schirmer I test was higher than that before the surgery. Corneal fluorescein sodium staining after surgery showed a decrease in score compared to the preoperative score. The BCVA of the patients after surgery increased significantly. The OSDI scores of patients with ankyloblepharon were significantly higher than for those without it. Postoperative symblepharon showed no significant difference compared to the preoperative symblepharon.

Conclusions: In this series, OCP patients with cataracts were able to undergo phacoemulsification surgery, whereas routine use of immunosuppression and closed postoperative follow-up were necessary.
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http://dx.doi.org/10.1155/2022/2496649DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9078804PMC
May 2022

Arsenic(iii) removal from aqueous solution using TiO-loaded biochar prepared by waste Chinese traditional medicine dregs.

RSC Adv 2022 Mar 9;12(13):7720-7734. Epub 2022 Mar 9.

State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences Wuhan 430074 P. R. China

Oxidation of As(iii) to As(v) is an effective way to improve the performance of most arsenic removal technologies. In this study, a new alternative biosorbent, TiO-loaded biochar prepared by waste Chinese traditional medicine dregs (TBC) was applied in remediation for As(iii) from aqueous solution. Compared with unmodified biochar, the specific surface areas and total pore volumes of TBC increased while the average aperture decreased due to the loading of nano-TiO. The X-ray diffraction (XRD) of TBC confirmed that the precipitated titanium oxide was primarily anatase. pH did not have a significant effect on the adsorption capacity at 10 mg L As(iii) in suspension with a pH ranging from 2 to 10. Adsorption kinetics data were best fitted by the pseudo-second-order model ( > 0.999). The Sips maximum adsorption capacity was 58.456 mg g at 25 °C, which is comparable with other adsorbents reported in previous literature. The Gibbs free energy (Δ) of As(iii) adsorption was negative, indicating the spontaneous nature of adsorption. The results of free radical scavenging and N purging experiments indicated that O acted as an electron accepter and O˙ dominated the oxidation of As(iii). The oxidation of As(iii) obviously affected the adsorption capacity for As(iii) by TBC. X-ray photoelectron spectroscopy (XPS) studies showed that As(iii) and As(v) existed on the surface of TBC, suggesting that the oxidation of As(iii) occurred. TBC played multiple roles for As(iii), including direct adsorption and photocatalytic oxidation adsorption. Regeneration and stability experiments showed that TBC was an environment-friendly and efficient adsorbent for As(iii) removal.
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http://dx.doi.org/10.1039/d1ra08941bDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8982158PMC
March 2022

Transient Antibiotic Tolerance Triggered by Nutrient Shifts From Gluconeogenic Carbon Sources to Fatty Acid.

Front Microbiol 2022 11;13:854272. Epub 2022 Mar 11.

Department of Energy, Environmental and Chemical Engineering, Washington University in St. Louis, Saint Louis, MO, United States.

Nutrient shifts from glycolytic-to-gluconeogenic carbon sources can create large sub-populations of extremely antibiotic tolerant bacteria, called persisters. Positive feedback in central metabolism was believed to play a key role in the formation of persister cells. To examine whether positive feedback in nutrient transport can also support high persistence to β-lactams, we performed nutrient shifts for from gluconeogenic carbon sources to fatty acid (FA). We observed tri-phasic antibiotic killing kinetics characterized by a transient period of high antibiotic tolerance, followed by rapid killing then a slower persister-killing phase. The duration of transient tolerance (3-44 h) varies with pre-shift carbon source and correlates strongly with the time needed to accumulate the FA degradation enzyme FadD after the shift. Additionally, FadD accumulation time and thus transient tolerance time can be reduced by induction of the glyoxylate bypass prior to switching, highlighting that two interacting feedback loops simultaneously control the length of transient tolerance. Our results demonstrate that nutrient switches along with positive feedback are not sufficient to trigger persistence in a majority of the population but instead triggers only a temporary tolerance. Additionally, our results demonstrate that the pre-shift metabolic state determines the duration of transient tolerance and that supplying glyoxylate can facilitate antibiotic killing of bacteria.
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http://dx.doi.org/10.3389/fmicb.2022.854272DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8963472PMC
March 2022

Gene interaction analysis of psoriasis in Chinese Han population.

Mol Genet Genomic Med 2022 05 30;10(5):e1858. Epub 2022 Mar 30.

Department of Dermatology, First Affiliated Hospital, Anhui Medical University, Hefei, China.

Background/aims: Psoriasis is a chronic immune-mediated inflammatory skin disease characterized by excessive proliferation of keratinocytes. It has a strong genetic predisposition; gene-gene interactions are important genetic models for common diseases. In this study, we explore pair-wise interactions among SNPs contributing to psoriasis susceptibility.

Methods: We first performed gene interactions with exome-sequencing, next, we analyzed gene interactions combining the exome sequencing data with the targeted sequencing data. After we sequenced HLA region, we analyzed gene interactions including HLA regions and non-HLA regions.

Results: We found interactions between HLA regions were significant. We observed significant interactions between HLA-C*06:02 and rs118179173 (snp31443520; p = 8.21 × 10 , OR = 0.22) and between HLA-C*06:02 and HLA-B:AA67 (p = 1.22 × 10 , OR = 0.45).

Conclusion: This study provides evidence that HLA is the most important susceptibility region on the risk of psoriasis and interactions that occur in this region are still significant.
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http://dx.doi.org/10.1002/mgg3.1858DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9034666PMC
May 2022

Induces Goat Endometrial Epithelial Cells Apoptosis via the Autophagy and Endoplasmic Reticulum Stress Pathway.

Animals (Basel) 2022 Mar 11;12(6). Epub 2022 Mar 11.

Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China.

Increasing evidence indicates that autophagy and endoplasmic reticulum (ER) stress are involved in the regulation of cell death; however, the role of autophagy and ER stress in -induced endometrial epithelial cell damage is still unelucidated. In the present study, our results showed that infection with increased the cytotoxicity and the protein expression of Bax, caspase-3, and cleaved-PARP-1 in goat endometrial epithelial cells (gEECs). Moreover, after infection, the expression of LC3II and autophagosomes were markedly increased. The autophagosome inhibitor 3-methyladenine (3-MA) significantly decreased the cytotoxicity and the expression of caspase-3, and cleaved-PARP-1; however, the autophagosome-lysosome fusion inhibitor chloroquine (CQ) increased their expression. Additionally, the protein expression of GRP78, EIF2α, and ATF4 were also markedly increased after infection. The ER stress inhibitor 4-PBA decreased the cytotoxicity and the expression of LC3II and apoptosis-related proteins in -infected gEECs. Collectively, our findings prove that the accumulation of autophagosomes exacerbated -induced gEECs apoptosis, and that ER stress was involved in the regulation of the autophagy and apoptosis.
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http://dx.doi.org/10.3390/ani12060711DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8944437PMC
March 2022

Study on the precipitation of iron and the synchronous removal mechanisms of antimony and arsenic in the AMD under the induction of carbonate rocks.

Environ Sci Pollut Res Int 2022 Aug 22;29(36):55161-55173. Epub 2022 Mar 22.

College of Resources and Environmental Engineering, Guizhou University, Guiyang, 550025, China.

The ecological environment can be severely polluted and destroyed by the acid mine drainage (AMD) generated during the exploration and utilization of minerals. However, neutralized by carbonate rocks (a natural material in Karst regions), the AMD secondary iron flocs containing a large number of iron oxides or hydroxide can be precipitated in AMD. The metal ions, such as antimony (Sb) and arsenic (As), can be effectively removed by these neutralizing products. In this paper, the neutralization reaction of different acid solutions in an iron-antimony-arsenic system was induced by carbonate rocks to explore the removal effect of metals during this neutralization process. Meanwhile, taking the release amounts of iron (Fe), Sb, and As as well as the phase transformation of minerals at different pH levels as stability indexes, we quantitatively analyzed the chemical stability of AMD neutralizing products (secondary iron flocs) containing Sb and As under the typical acid-base environment (pH = 3.0 ~ 9.0) of AMD and other waters. Results showed that the neutralization reaction with carbonate rocks induced the co-precipitation of Fe with Sb and As. When the concentration ratio of Fe, Sb, and As was 30:1:1, the pH of AMD raised from 3.0 to 7.28 within 72 h, and the three elements were removed by 99%, 85%, and 90%, respectively. After soaking the AMD secondary iron flocs in an acid environment (pH = 3.0) for 30 days, the release amount of Fe reached its peak of 0.070 mg/g. Then, when the pH value increased to 4.0, the As and Sb showed their maximum release amounts of 14.90 µg/g and 19.19 µg/g, respectively. In addition, under acidic conditions, these AMD secondary iron flocs were easily transformed into the goethite with better crystallinity and higher structural stability. This study could help reveal the development of the secondary mineral during the treatment of AMD by carbonate rocks and understand the release characteristics of metals from AMD secondary products containing Sb and As, which sheds light on and provides theoretical foundations for the passive treatment of AMD containing these two elements in the future.
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http://dx.doi.org/10.1007/s11356-022-19728-4DOI Listing
August 2022

Dynamic ellipsometry measurement based on a simplified phase-stable dual-comb system.

Opt Express 2022 Feb;30(5):7806-7820

Spectroscopic ellipsometry is a powerful tool for characterizing thin film, polarization optics, semiconductors, and others. Conventional approaches are subject to restrictions of mechanical instability and measurement speed. The complex locking scheme of previous dual-comb spectroscopic ellipsometry belies its practicability. We present and demonstrate here dynamic spectroscopic ellipsometry based on a simplified phase-stable dual-comb system, which could realize the online dynamic measurement of optical properties of materials. A precision of 1.31 nm and a combined uncertainty of 13.80 nm (k = 2) in the thickness measurement of thin-film samples has been achieved. Moreover, the dynamic performance of the system is investigated under a high data acquisition rate (1 kHz) with a dynamic resolution of ellipsometric parameter better than 0.1 rad.
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http://dx.doi.org/10.1364/OE.453406DOI Listing
February 2022

Genome-wide analyses of nonsyndromic cleft lip with or without palate identify 20 new risk loci in the Chinese Han population.

J Genet Genomics 2022 Feb 22. Epub 2022 Feb 22.

Department of Dermatology, The First Affiliated Hospital of Anhui Medical University, Hefei, China; Institute of Dermatology, Anhui Medical University, Hefei, China; Key Laboratory of Dermatology, Ministry of Education, Anhui Medical University, Hefei, China; Inflammation and Immune Mediated Diseases Laboratory of Anhui Province, Hefei, China; Anhui Provincial Institute of Translational Medicine, Hefei, China. Electronic address:

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http://dx.doi.org/10.1016/j.jgg.2022.02.004DOI Listing
February 2022

Size Effect of MgO on the Ionic Conduction Properties of a LiBH·1/2NH-MgO Nanocomposite.

ACS Appl Mater Interfaces 2022 Feb 10;14(7):8947-8954. Epub 2022 Feb 10.

Institute of New Energy and Low-Carbon Technology, Sichuan University, Chengdu 610065, China.

A solid-state electrolyte (SSE) is the core component for fabricating solid-state batteries competitive with the currently commercial Li-ion batteries. In the present study, a LiBH·1/2NH-MgO nanocomposite has been developed as a fast Li-ion conductor. The conductive properties depend strongly on the size of MgO nanopowders. By adding MgO nanoparticles, the first-order transition at 55 °C observed in the crystalline LiBH·1/2NH is suppressed due to the conversion of LiBH·1/2NH into the amorphous state. When the size of MgO decreases from 163.6 to 13.9 nm, the MgO amount required for the phase-transition suppression of LiBH·1/2NH decreases linearly from 92 to 75 wt %, accompanied by a significant enhancement of ionic conductivity. The optimized nanocomposite with 75 wt % MgO of size 13.9 nm exhibits a pronouncedly high conductivity of 4.0 × 10 S cm at room temperature, which is 20 times higher than that of the crystalline LiBH·1/2NH. Furthermore, a smaller size MgO contributes to a higher electrochemical stability window (ESW) owing to the stronger interfacial interaction B-O bonds, , an ESW of 4.0 V is achieved with the addition of 75 wt % MgO of size 13.9 nm.
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http://dx.doi.org/10.1021/acsami.1c20976DOI Listing
February 2022

Association between anti-fibrillin-2 protein induced retinal degeneration via intravitreous delivery and activated TGF-β signalling in mice.

Clin Exp Pharmacol Physiol 2022 05 2;49(5):586-595. Epub 2022 Mar 2.

Department of Ophthalmology and Optometry, Shandong University of Traditional Chinese Medicine, Jinan, China.

Fibrillin-2 (FBN2) is a major component of tissue microfibrils, and the decrease of FBN2 perturbs the signalling events mediated by transforming growth factor-β (TGF-β), thereby playing a role in macular degeneration. However, the association between the retinal degeneration resulting from the abnormality of FBN2 and the activation of TGF-β signalling has not been fully addressed. In the present study, the mice were divided into a normal control group (NC group), a phosphate-buffered saline (PBS) injection group (PBS group), and an anti-FBN2 protein injection group (anti-FBN2 group), and the mice in PBS and anti-FBN2 groups received the relevant treatment via the intravitreal injection once a week for three consecutive weeks. One week later after injection, the retinal morphology and visual function of the fundus were detected. Further, the expression of FBN2, TGF-β1, TGF-β2 and TGF-β3 in retina was measured using quantitative polymerase chain reaction and enzyme-linked immunosorbent assay (ELISA), respectively. As a result, fundus examination suggests that after intravitreous injection of anti-FBN2 protein, there were a large patchy yellow white degeneration region and numerous pigmentations in the retina in anti-FBN2-treated mice; by contrast, there was no apparent change in mice from the NC and PBS groups. The retina suffered markedly damage, and the thickness of whole retina and outer nuclear layer markedly thinned. The expression of FBN2 was decreased whereas the levels of TGF-β1, TGF-β2 and TGF-β3 were upregulated. Together, our findings indicate that the intravitreous delivery of anti-FBN2 protein could induce retina degeneration in mice, accompanied by the higher activated TGF-β. The retinal degeneration mouse model established will provide a platform for the investigation of the retinal diseases.
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http://dx.doi.org/10.1111/1440-1681.13631DOI Listing
May 2022

Review of Early Signs of Breast Implant Infection.

Aesthetic Plast Surg 2022 Jan 29. Epub 2022 Jan 29.

Plastic Surgery Division, Department of Surgery, John A. Burns School of Medicine, University of Hawaii, 1329 Lusitana Street, Suite 807, Honolulu, Hawaii, 96813, USA.

Background: Breast implant infection remains a problem despite various prevention methods. Common pathogens such as staphylococcus aureus typically present acutely with fever, erythema, swelling, and pain. However, with the increasing occurrence of mycobacterial infections, associated early signs and symptoms are typically absent, making early detection difficult.

Method: A retrospective chart review of patients who underwent esthetic breast augmentation by senior surgeon FDP in an outpatient surgical facility from 1992 to 2021 was conducted. Cases of infections and related information including infection onset, presenting signs and symptoms, and culture results were recorded. Presentation differences between infections caused by common pathogens and mycobacteria were compared.

Results: A total of nine infections were reported in 365 patients. Seven cases were due to common pathogens: staphylococcus aureus, pseudomonas aeruginosa, methicillin-resistant staphylococcus aureus (MRSA), and staphylococcus epidermidis. Fever, erythema was presented within an average of 10 days after surgery. Two cases were due to mycobacterium chelonae (non-tuberculous mycobacterium) infection. In the latter, the earliest signs and symptoms consisted of mild pain and swelling in the absence of fever and erythema, which began on average of 54 days after the operation.

Conclusion: Common breast implant infection pathogens usually present early with fever and erythema. However, our study found that non-tuberculous mycobacteria infections occur much later and fever and erythema are typically absent. The purpose of this study is to increase the awareness of clinicians with this rare but rapidly increasing variety of infections and to advise the operating surgeon to include mycobacteria infections in the differential diagnosis.

Level Of Evidence Iv: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
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http://dx.doi.org/10.1007/s00266-021-02702-9DOI Listing
January 2022

Fine Mapping Analysis of the MHC Region to Identify Variants Associated With Chinese Vitiligo and SLE and Association Across These Diseases.

Front Immunol 2021 10;12:758652. Epub 2022 Jan 10.

Department of Dermatology, The First Affiliated Hospital of Anhui Medical University, Hefei, China.

The important role of MHC in the pathogenesis of vitiligo and SLE has been confirmed in various populations. To map the most significant MHC variants associated with the risk of vitiligo and SLE, we conducted fine mapping analysis using 1117 vitiligo cases, 1046 SLE cases and 1693 healthy control subjects in the Han-MHC reference panel and 1000 Genomes Project phase 3. rs113465897 (P=1.03×10, OR=1.64, 95%CI =1.44-1.87) and rs3129898 (P=4.21×10, OR=1.93, 95%CI=1.66-2.25) were identified as being most strongly associated with vitiligo and SLE, respectively. Stepwise conditional analysis revealed additional independent signals at rs3130969(p=1.48×10, OR=0.69, 95%CI=0.60-0.79), HLA-DPB1*03:01 (p=1.07×10, OR=1.94, 95%CI=1.49-2.53) being linked to vitiligo and HLA-DQB1*0301 (P=4.53×10, OR=0.62, 95%CI=0.52-0.75) to SLE. Considering that epidemiological studies have confirmed comorbidities of vitiligo and SLE, we used the GCTA tool to analyse the genetic correlation between these two diseases in the HLA region, the correlation coefficient was 0.79 (P=5.99×10, SE=0.07), confirming their similar genetic backgrounds. Our findings highlight the value of the MHC region in vitiligo and SLE and provide a new perspective for comorbidities among autoimmune diseases.
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http://dx.doi.org/10.3389/fimmu.2021.758652DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8784546PMC
February 2022

A DNA tetrahedron docking assembly for imaging telomerase activity in cancerous cells.

Anal Chim Acta 2022 Feb 24;1193:339395. Epub 2021 Dec 24.

School of Chemistry and Chemical Engineering, Shandong University, 250100, Jinan, PR China. Electronic address:

The detection of telomerase activity inside cells is valuable for early cancer diagnosis and telomerase function study. However, besides cancerous cells, telomerase is also found to be expressed in few non-cancerous cells, which influences the assay reliability. By virtue of the extracellular pH, we design a DNA tetrahedron docking assembly (DTDA) for only responding telomerase activity in cancerous cells. The DTDA maintains structural integrity with extracellular acid pH of cancerous cells, but releases a telomerase substrate-containing strand after its cell internalization due to intracellular alkaline pH. The strand gets elongated by intracellular telomerase, docks to the vertex of tetrahedron, and returns to the DTDA after its separation, accompanied by fluorescence enhancement. For non-cancerous cells, the telomerase substrate-containing strand is already dissociated with extracellular alkaline pH and cannot enter into cells to achieve subsequent docking event. DTDA well distinguishes cancerous cells from non-cancerous cells in which telomerase are both expressed. The strategy can provide a more reliable way for telomerase-based cancer diagnosis and telomerase oncogenic study.
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http://dx.doi.org/10.1016/j.aca.2021.339395DOI Listing
February 2022

Clinical outcome of phacoemulsification combined with intraocular lens implantation for primary angle closure/glaucoma (PAC/PACG) with cataract.

Am J Transl Res 2021 15;13(12):13498-13507. Epub 2021 Dec 15.

Shannxi Provincial People's Hospital Xi'an 710038, Shaanxi, China.

Objective: To evaluate the efficacy of phacoemulsification (Phaco) combined with intraocular lens implantation for treatment of primary angle-closure glaucoma (PACG) patients with cataract.

Methods: A total of 62 patients treated in our hospital meeting the inclusion criteria were included, including 62 eyes (26 PAC eyes and 36 PACG eyes). PACG patients were divided into early, middle, and advanced stages based on the HPA visual field staging system. The subjects were also grouped according to the extent of peripheral anterior synechia (PAS). Patients received topical medical treatment preoperatively and Phaco performed by the same surgeon. The visual acuity, intraocular pressure (IOP), anterior chamber depth (ACD), medication used, visual field and retinal nerve fiber layer (RNFL) were observed before and 6-24 months after surgery.

Results: The mean age of the patients was 68±8.91 years old, and postoperative follow-up was 13.1±5.5 months. Postoperative visual acuity was improved in all patients (P<0.001). Postoperatively, the IOP decreased significantly (P<0.001), the number of medications was reduced (P<0.001), and the ACD was deeper than that before operation (P<0.001). There was no significant difference in visual field (P=0.973) or RNFL (P=0.268) after surgery during the follow-up. There was no statistical difference in postoperative changes of various indexes between PAC and PACG patients. The decrease of IOP in patients with early stage PACG was significantly higher than that in patients in the middle and advanced stages (F=3.519, P=0.041), and the number of medications used in early-stage PACG patients was also significantly lower than that of advanced patients (P=0.020). There was no significant difference in postoperative visual acuity (X=0.139, P=0.987) or IOP decline (F=0.260, P=0.854) among patients with different extents of preoperative PAS, nor was there any correlation between postoperative IOP control and preoperative PAS. No serious complications were observed in any subject.

Conclusion: In PAC/PACG patients, Phaco can significantly control IOP, and prevent visual field defect and progressive loss of RNFL, indicating that the procedure has a protective effect on the optic nerve. Phaco is more effective in the treatment of early stage PACG than in middle or advanced stage, and can be used in PAC/PACG patients with different extents of PAS, but close follow-up is necessary.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8748130PMC
December 2021

RNA-binding protein p54/NONO potentiates nuclear EGFR-mediated tumorigenesis of triple-negative breast cancer.

Cell Death Dis 2022 01 10;13(1):42. Epub 2022 Jan 10.

Department of Nuclear Medicine, Institute of Clinical Nuclear Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, 200127, Shanghai, China.

Nuclear-localized epidermal growth factor receptor (EGFR) highly correlates with the malignant progression and may be a promising therapeutic target for breast cancer. However, molecular mechanisms of nuclear EGFR in triple-negative breast cancer (TNBC) have not been fully elucidated. Here, we performed gene-annotation enrichment analysis for the interactors of nuclear EGFR and found that RNA-binding proteins (RBPs) were closely associated with nuclear EGFR. We further demonstrated p54/NONO, one of the RBPs, significantly interacted with nuclear EGFR. NONO was upregulated in 80 paired TNBC tissues and indicated a poor prognosis. Furthermore, NONO knockout significantly inhibited TNBC proliferation in vitro and in vivo. Mechanistically, NONO increased the stability of nuclear EGFR and recruited CREB binding protein (CBP) and its accompanying E1A binding protein p300, thereby enhancing the transcriptional activity of EGFR. In turn, EGFR positively regulated the affinity of NONO to mRNAs of nuclear EGFR downstream genes. Furthermore, the results indicated that the nuclear EGFR/NONO complex played a critical role in tumorigenesis and chemotherapy resistance. Taken together, our findings indicate that NONO enhances nuclear EGFR-mediated tumorigenesis and may be a potential therapeutic target for TNBC patients with nuclear EGFR expression.
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http://dx.doi.org/10.1038/s41419-021-04488-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8748691PMC
January 2022

Resveratrol and lycium barbarum polysaccharide improve Qinling giant panda (Ailuropoda melanoleuca Qinlingensis) sperm quality during cryopreservation.

BMC Vet Res 2022 Jan 7;18(1):23. Epub 2022 Jan 7.

College of Veterinary Medicine, Northwest A & F University, Yangling, 712100, Shaanxi, China.

Background: Semen cryopreservation has become an essential tool for conservation efforts of the giant panda (Ailuropoda melanoleuca); however, it is severely detrimental to sperm quality. Evidence has shown that antioxidants have the potential to reverse cryopreservation-induced damage in sperm. The purpose of this study was to screen effective antioxidants that could retain sperm quality during cryopreservation and to determine the optimal dose. Seven antioxidant groups, including resveratrol (RSV = 50 μM, RSV = 100 μM, RSV = 150 μM), lycium barbarum polysaccharide (LBP = 2 mg/mL, LBP = 4 mg/mL), laminaria japonica polysaccharides (LJP = 1 mg/mL) or combination (LBP = 2 mg/mL, LJP = 1 mg/mL and RSV = 100 μM) were assessed.

Results: RSV, LBP, LJP, or a combination of RSV, LBP, and LJP added to the freezing medium significantly improved sperm progressive motility, plasma membrane integrity, acrosome integrity, and mitochondrial activity during the cryopreservation process. Furthermore, the activities of glutathione peroxidase and superoxide dismutase were also improved. The levels of reactive oxygen species and malondialdehyde in semen were notably reduced. Hyaluronidase activity and acrosin activity were significantly increased in LBP-treated sperm. However, sperm total motility and DNA integrity were not significantly different between the groups.

Conclusions: RSV (50 μM) or LBP (2 mg/mL) are the best candidate antioxidants for inclusion in the freezing medium to improve the quality of giant panda spermatozoa during semen cryopreservation.
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http://dx.doi.org/10.1186/s12917-021-03122-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8739993PMC
January 2022

Fine-Mapping of the Major Histocompatibility Complex Region Linked to Leprosy in Northern China.

Front Genet 2021 16;12:768259. Epub 2021 Dec 16.

Department of Dermatology, No. 1 Hospital, Anhui Medical University, Hefei, China.

Leprosy is a chronic infectious skin and neurological disease, and genetic background is considered to be one of the major factors of risk. The major histocompatibility complex (MHC) region not only affects susceptibility to leprosy but also its development and outcome. Given the complex traits of the MHC region, variants and the potential mechanism by which HLA influences leprosy development need to be further explored. We extracted previous genome-wide association study data from the Northern Han Chinese population to perform HLA fine-mapping. Using the 1,000 Genome Project Phase 3 dataset as the reference panel, single-nucleotide polymorphisms (SNP), insertion and deletion (INDEL) and copy number variant (CNV) imputation were carried out. HLA classical alleles and amino acids in the MHC region were imputed using the HAN-MHC database. Further stepwise regression analysis was conducted to analyze independent signals of variants related to leprosy. We identified four independent variants: esv3608598, rs7754498, rs3130781 and rs144388449. Among them, esv3608598 is a CNV and the first HLA CNV associated with leprosy risk. SNP annotation using RegulomeDB, HaploReg, and rVarBase showed that three SNPs are likely to affect the pathogenesis of leprosy. In summary, this is the first study to assess the association between HLA CNV and leprosy susceptibility in a Northern Han Chinese population. By fine mapping of the MHC region in this population, our findings provide evidence for the contribution of HLA to leprosy susceptibility.
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http://dx.doi.org/10.3389/fgene.2021.768259DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8716717PMC
December 2021

A SERS aptasensor based on porous Au-NC nanoballoons for Staphylococcus aureus detection.

Anal Chim Acta 2022 Jan 15;1190:339175. Epub 2021 Oct 15.

Chongqing Key Laboratory of Natural Product Synthesis and Drug Research, School of Pharmaceutical Sciences, Chongqing University, 55 Daxuecheng South Road, 401331, Shapingba, Chongqing, PR China. Electronic address:

In this work, we developed a new approach for fabricating hollow and porous nitrogen doped carbon nanoballoons loading AuNPs (Au-NC-NBs) with a large specific surface area, a high N and Au content. The surface-enhanced Raman scattering (SERS) aptasensor based on the resulting Au-NC-NBs possess a wider linear range (10 to 10 cells/mL), a lower detection limit (3 cells/mL), better selectivity for detecting bacteria than previously reported sensors. Importantly, Au-NC-NBs SERS aptasensor also exhibits excellent performance for detecting bacteria in the real food and biological samples. This work provides a facile and versatile designing strategy for controlled construction of SERS biosensor by combination of Au nanoparticles and carbon materials, which has a great applied potential in food safety monitoring and clinical diagnosis.
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http://dx.doi.org/10.1016/j.aca.2021.339175DOI Listing
January 2022

The anti-fatigue activity of corn peptides and their effect on gut bacteria.

J Sci Food Agric 2022 Jun 16;102(8):3456-3466. Epub 2021 Dec 16.

Beijing Engineering Research Center of Protein and Functional Peptides, China National Research Institute of Food and Fermentation Industries Co. Fermentation Industries Co. Ltd, Beijing, China.

Background: Corn peptides (CPs) are rich in branched-chain amino acids such as leucine and have a variety of biological activities such as antioxidant and improved lipid distribution. In this article, we prepared CPs by enzymatic digestion of corn proteins and evaluated their anti-fatigue activity.

Results: We evaluated the anti-fatigue effect of CPs through an exhaustive swimming experiment. The results showed that CPs were able to significantly reduce the rate of body weight gain and prolong the duration of exhaustive swimming. Besides, CPs reduced blood urea nitrogen (BUN) levels after exercise, while they significantly increased muscle glycogen and liver glycogen stores. They reduced muscle cell damage from exercise. In addition, CPs were effective in increasing AMPK, PGC-1α and PI3K protein expression levels and promoting Akt phosphorylation. Correlation analysis showed that CPs increased the abundance of probiotics such as Lactobacillus and Akkermansia in the gut microflora.

Conclusion: CPs, which enhanced exercise performance in mice and could modulate gut microbial composition, had significant anti-fatigue activity. © 2021 Society of Chemical Industry.
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http://dx.doi.org/10.1002/jsfa.11693DOI Listing
June 2022

Transcriptomic Analysis of STAT1/3 in the Goat Endometrium During Embryo Implantation.

Front Vet Sci 2021 14;8:757759. Epub 2021 Oct 14.

College of Veterinary Medicine, Northwest A&F University, Xianyang, China.

Interferon tau (IFNT), a pregnancy recognition signal in ruminants, promotes the establishment of embryo implantation by inducing the expression of interferon-stimulated genes (ISGs) via the Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway. However, the precise regulatory mechanism of IFNT in goat embryo implantation remains largely unknown. In this study, we performed RNA sequencing of goat endometrial epithelial cells (gEECs) with or without 20 ng/mL IFNT treatment. Differential comparison showed that there were 442 upregulated differentially expressed genes (DEGs) and 510 downregulated DEGs. Bioinformatic analyses revealed that DEGs were significantly enriched in immune-related functions or pathways. The qRT-PCR validation results showed that the expression levels of STAT family members (STAT1, STAT2, and STAT3) were significantly upregulated in gEECs after IFNT treatment, which is in agreement with the RNA-seq data. Meanwhile, the protein levels of p-STAT1 and p-STAT3 increased significantly in gEECs after 6 and 24 h of IFNT treatment, respectively. Further experiments also confirmed that both mRNA and protein phosphorylation levels of STAT1 and STAT3 in the uterus on day 18 of pregnancy (P18) were significantly increased compared to those on day 5 (P5) and day 15 of pregnancy (P15). On P5, STAT1 and STAT3 proteins were primarily located in the uterine luminal epithelium (LE) and glandular epithelium (GE), and were also detected in the stromal cells. The intense immunostaining of STAT1 and STAT3 proteins were decreased on P15 and then increased on P18, especially in the superficial GE and subepithelial stromal cells. Moreover, p-STAT1 and p-STAT3 were highly expressed in the deep GE on P18. Collectively, these results highlight the role of IFNT in regulating endometrial receptivity in gEECs and uncover the temporal and spatial changes in the expression of STAT1/3 during embryo implantation in the goat endometrium.
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http://dx.doi.org/10.3389/fvets.2021.757759DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8551392PMC
October 2021

Genome-wide meta-analysis identifies ten new psoriasis susceptibility loci in the Chinese population.

J Genet Genomics 2022 02 22;49(2):177-180. Epub 2021 Oct 22.

Department of Dermatology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230031, China; Key Laboratory of Dermatology (Anhui Medical University), Ministry of Education, Hefei 230031, China; Anhui Province Laboratory of Inflammation and Immune Mediated Diseases 230031, China; Anhui Provincial Institute of Translational Medicine 230031, China. Electronic address:

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http://dx.doi.org/10.1016/j.jgg.2021.10.001DOI Listing
February 2022

ISGylation Inhibits an LPS-Induced Inflammatory Response via the TLR4/NF-κB Signaling Pathway in Goat Endometrial Epithelial Cells.

Animals (Basel) 2021 Sep 3;11(9). Epub 2021 Sep 3.

College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China.

Endometritis is a common and important reproductive disease of domestic animals. The principal factors responsible for the disease are infection with Gram-negative bacteria, the release of Lipopolysaccharides (LPS) and activation of the TLR4/NF-κB signaling pathway. However, we do not fully understand the interaction between endometrial immunity and bacterial infection in the disease etiology. The ubiquitin-like protein ISG15 can regulate the TLR4/NF-κB signaling pathway via the ISGylation modification system, modulating the inflammatory response. In the present study, we found that ISG15 protein was expressed mainly in the cytoplasm of goat endometrial epithelial cells (gEECs) and that the expression of key genes and proteins of ISGylation increased in LPS-induced gEECs. Overexpression and silencing of the gene demonstrated that ISGylation inhibited an LPS-induced inflammatory response via the TLR4/NF-κB signaling pathway in gEECs. Here, we provide the experimental basis for further exploration of the role of the ISGylation modification system in the inflammatory response of endometrium and a potential method for the treatment of endometritis.
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http://dx.doi.org/10.3390/ani11092593DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8470639PMC
September 2021

Preparation of Targeted Mitochondrion Nanoscale-Release Peptides and Their Efficiency on Eukaryotic Cells.

J Biomed Nanotechnol 2021 Aug;17(8):1679-1689

Xi'an Medical University, Xi'an 710021, Shaanxi, PR China.

We established a self-decomposable SiO₂ encapsulated mitochondrial targeting short peptide SS31 drug loading system (SiO₂@SS31) to determine its nano-sustained release characteristics in eukaryotic cells. We explored the protection of SiO₂@SS31 on the 661W cells after oxidative injury by H₂O₂. After the drug loading, we detected the morphology of SiO₂@SS31 by transmission electron microscopy (TEM). Moreover, high-pressure liquid chromatography (HPLC) was used to determine the drug capacity and encapsulation efficiency of the nanoparticles. Then, the release curve in vitro was drawn. The 661W cells were cultured in vitro to allow the detection of cytotoxicity by the MTT assay. The SS31loaded nanoscale microspheres labeled with fluorescein isothiocyanate (SiO₂@FITC-SS31) were prepared, and their sustained release effect was detected with intracellular endocytosis, using confocal microscopy and flow cytometry. Within 15 days, the [email protected] nanoparticles were completely decomposed and simultaneously released the SS31 peptide in deionized water and normal saline. Nonetheless, the process was faster in simulated body fluid and serum. The MTT assay suggested that SiO₂@SS31 has sustained protection compared with SS31 in the 661W cells at 48 h. Flow cytometry proved SiO₂@FITC-SS31 could maintain a high level and last longer after 24 h. The SS31 peptide, which has excellent medical application prospects, can be slowly and continuously released from self-decomposable SiO₂ and targeted to concentrate on mitochondria.
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http://dx.doi.org/10.1166/jbn.2021.3141DOI Listing
August 2021
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