Publications by authors named "Rui-Qing Wang"

23 Publications

  • Page 1 of 1

[Question and thinking on study of acupuncture-moxibustion academic school].

Zhongguo Zhen Jiu 2021 Jun;41(6):663-5

College of Acupuncture-Moxibustion and Tuina, Hebei University of TCM, Shijiazhuang 050200, China.

The concepts of acupuncture-moxibustion "academic school" "school of thought" and "school" are differentiated. It is believed that "academic school" is the general term of "school of thought" and "school". This paper puts forward a solution to the confusion of related concepts, disunity of formation conditions, disunity of divisionmethods and disunity of nomenclature. It is proposed that a great concentration should be put on the arrangement of acupuncture-moxibustion academic school and the regulation on related issues. Because of the complexity and diversity of the form and content of acupuncture-moxibustion schools, the division of them should not be so specified and the unification of related issues should not be so strictly required.
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http://dx.doi.org/10.13703/j.0255-2930.20201223-k0002DOI Listing
June 2021

[Master DOU Han-qing's academic achievements in acupuncture and moxibustion].

Zhen Ci Yan Jiu 2021 May;46(5):426-30

Institute of Acupuncture-moxibustion and Massage, Hebei University of Traditional Chinese Medicine, Shijiazhuang 050200, China.

Master DOU Han-qing is one famous acupuncturist in the Jin and Yuan Dynasties of China. In consideration of his newly neatened works (,《》), (,《》) and (,《》) which were not paid enough attention in the past, we make a systematic and comprehensive summary about his academic achievements in the present paper. There are six main aspects about Dou's academic achievements: blending the essence of sages' acupuncture theories and clinical experience, correcting predecessors' mistakes; innovating the needlingmethods; expanding the clinical applications of acupoints and paying attention to combination of meridian acupoints and extra-points in clinical practice; inheriting and developing acupuncture prescriptions in the Song Dynasty, creating new diagnostic and treatment modes; initiating needling techniques of various schools in the Ming Dynasty; and describing the contents of acupuncture and moxibustion in the form of odes. Moreover, we also put forward some of our enlightenments and considerations about DOU Han-qing's academic achievements.
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http://dx.doi.org/10.13702/j.1000-0607.200755DOI Listing
May 2021

[Characteristics of clinical application of electroacupuncture therapy for peripheral neuropathy based on data mining].

Zhen Ci Yan Jiu 2021 Mar;46(3):240-7

Institute of Acupuncture-moxibustion and Massage, Hebei University of Chinese Medicine, Shijiazhuang 050200, China.

Objective: To investigate the application characteristics of electroacupuncture (EA) in the treatment of peripheral neuropathy, so as to provide a basis for clinical use of EA therapy.

Methods: Keywords of "electroacupuncture"peripheral neuropathy" "facial paralysis" "trigeminal neuralgia" "sciatica" "common peroneal nerve injury" "diabetic peripheral neuropathy" "intercostal neuralgia" "gluteal epithelial neuritis" "ulnar nerve injury" "median nerve paralysis" "postherpetic neuralgia", and "great occipital neuralgia" were used to search articles in both English and Chinese published from 1999 to 2019 in databases of CNKI, Wanfang, VIP, CBM, Ovid, PubMed and Embase and related books such as electroacupuncture, and neurology, followed by establishing a database of "Electroacupuncture Treatment of Peripheral Neuropathy". Then, the collected articles were put into statistical analysis after sorting, screening, input, checking, and data extracting by using data mining technology and statistical software EpiData.

Results: Of the searched 1 528 papers, 778 were eligible, involving 13 types of peripheral neuropathy which the facial paralysis and facial spasm were most frequently seen, followed by trigeminal neuralgia and sciatica, with an effective rate being above 90% for nearly all the 13 diseases. The acupoints employed were chiefly those close to the affected area and distribute along the nerve trunk.In addition, about the needling techniques, the penetration needling was frequently used, and the triple needling, quintuple needling and accompanied needling were also used. Regarding the related needle manipulations, the uniform reinforcing-reduction technique was most frequently used. The duration of EA was 30 min, with a highest stimulating frequency of 50 Hz. The acupoint injection was frequently supplemented, followed by moxibustion, and the treatment sessions were usually about 30 times.

Conclusion: EA therapy is frequently used in the treatment of peripheral neuropathy, and has some characteristics in acupoint selection, stimulating parameters and some additional needling techniques.
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http://dx.doi.org/10.13702/j.1000-0607.200638DOI Listing
March 2021

[Comparison of different schools of hand acupuncture therapies].

Zhongguo Zhen Jiu 2020 Nov;40(11):1223-8

School of Acupuncture-Moxibustion and Tuina, Hebei University of CM, Shijiazhuang 050200, China.

The historical origin of hand acupuncture therapies were collected. The positioning method, acupuncture manipulation and dominant diseases, etc. were compared among 8 kinds of extensively used hand acupuncture schools, such as traditional hand acupuncture, Korean finger needle and 's hand image needle. It is found that the positioning method and needling method are not unified and the dominant diseases not specified. In the future study, the regimen of hand acupuncture should be optimized, the optimal holographic image, acupuncture manipulation and quantitative standards should be developed and the integration among schools should be promoted so that the relevant specifications can be formulated, national standards be developed and the application and development of hand acupuncture therapy be promoted.
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http://dx.doi.org/10.13703/j.0255-2930.20190828-k0007DOI Listing
November 2020

[Study on characteristics and regularity of acupuncture prescription by ].

Zhongguo Zhen Jiu 2021 Jan;41(1):105-9

College of Acupuncture-Moxibustion and Tuina, Hebei University of CM, Shijiazhuang 050200, China.

Summarized the characteristics and rules of -'s acupuncture prescription in ancient acupuncture and moxibustion literature, and provided reference for studying -'s academic thought and modern acupuncture clinic. The literature of 's acupuncture prescription was collected, sorted out and input, the database of -s acupuncture prescription was established, the frequency of acupoints in the prescription was analyzed, and the Apriori algorithm was used to analyze the rule of acupoints in the prescription. A total of 446 -s acupuncture prescriptions are included, and the maximum number of acupoints in acupuncture prescriptions is 12. The most common diseases are internal medicine syndrome, pain syndrome of head, face and body and five sense organs disease. In all the prescriptions of -, 205 acupoints are used in total, including 25 extra-meridian points. There are 7 groups of acupoint pairs with the strongest correlation in -'s acupuncture prescription, including Hegu (LI 4) - Zusanli (ST 36), Zusanli (ST 36) - Zhongwan (CV 12), Hegu (LI 4) - Quchi (LI 11), Hegu (LI 4)-Weizhong (BL 40), Quchi (LI 11) - Jianjing(GB 21), Weizhong (BL 40) - Quchi (LI 11) and Weizhong (BL 40)-Zusanli (ST 36). The principle of acupoint selection mainly includes near acupoint selection and far acupoint selection. In the method of matching acupoints, above and below the matching acupoints, this meridian matching acupoints and inner and outer meridian matching acupoints are used. pays attention to the coordination of acupuncture and moxibustion methods with acupoints and emphasizes acupuncture sequence.
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http://dx.doi.org/10.13703/j.0255-2930.20200703-k0002DOI Listing
January 2021

[Regularities of acupoint combinations and characteristics of core acupoint application for acupuncture-moxibustion treatment of mammary gland hyperplasia based on complex network analysis].

Zhen Ci Yan Jiu 2021 Jan;46(1):76-83

Institute of Acupuncture-moxibustion and Massage, Hebei University of Traditional Chinese Medicine, Shijiazhuang 050200, China.

Objective: To analyze the acupoint combination regularities and application characteristics of core acupoints in acupuncture-moxibustion treatment of mammary gland hyperplasia (MGH) by using complex network technology, so as to provide reliable evidence for clinical selection of acupoints and treatment ideas.

Methods: The articles related to acupuncture treatment of MGH published from January of 1981 to May of 2020 were collected from databases as China National Knowledge Infrastructure (CNKI), Wanfang Data Knowledge Service Platform (Wanfang), Chinese Journal of Science and Technology of VIP (VIP), PubMed, the Excerpta Medica Database (EMBASE), Cochrane Library, and Web of Science and Ovid database (OVID) according to the set inclusion and exclusion criteria of the present paper and by using keywords of "mammary gland hyperplasia", "mastalgia" or "fibrocystic breast change", "breast cystic hyperplasia ", etc. plus "acupuncture", "moxibustion", etc. Then, a correlative database model was established by using SPSS Modeler 18.0 to analyze their association regularities, followed by conducting a complex network analysis with Gephi 0.9.2 software.

Results: A total of 312 eligible articles containing 343 acupoint prescriptions and 113 acupoints were collected. The total frequency of use of the 113 acupoints was 1 998. The association regularity analysis showed a top relativity between Danzhong(CV17) and Zusanli(ST36). The analysis of the complex network topology revealed that CV17, Qimen(LR14), Sanyinjiao(SP6), Taichong(LR3), ST36, Jianjing(GB21), Rugen(ST18), Wuyi(ST15), Neiguan(PC6), Ashi-points, Fenglong (SP40), Guanyuan(CV4), Taixi(KI3), Tianzong(SI11), Ganshu (BL18), and Hegu(LI14) are the core acupoints for treatment of MGH. The principle for composing acupoint prescriptions is mainly the combination of acupoints on the left and right sides, the upper and lower parts, front and back parts of the body, respectively, and those of the outer and inner meridians, and those of the same name meridians and Zangfu-organ syndrome differentiation. The needle-insertion direction is mostly toward the focus.

Conclusion: The composition of acupoint prescriptions for treating MGH with acupuncture and moxibustion is mainly based on the combination of specific acupoints among which the confluent acupoints are most frequently used, followed by the combination of acupoints distributing at the chest, back, upper and lower limbs, and the local acupoints.
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http://dx.doi.org/10.13702/j.1000-0607.200696DOI Listing
January 2021

[Comparison and analysis of different schools of Chinese auricular acupuncture].

Zhongguo Zhen Jiu 2020 Dec;40(12):1363-8

College of Acupuncture-Moxibustion and Tuina, Hebei University of CM, Shijiazhuang 050200, China.

Based on the literature regarding auricular acupuncture in recent 60 years, the theoretical basis, acupuncturemethods and dominant diseases are analyzed and compared, and three major auricular acupuncture schools are proposed. The first school is the sinicized auricular acupuncture school derived from French Nogier auricular acupuncture school, which can be further divided into three branches: 's auricular acupuncture school, 's auricular acupuncture school and 's auricular acupuncture school. The second school is auricular acupuncture school inherited and developed from classical Chinese auricular acupuncture. The third school is 's auricular acupuncture school which is based on Chinese and western medical theories but different from the embryo inversion theory of Nogier auricular acupuncture school. Furthermore, the similarities and differences of the theoretical sources, the distribution characteristics of auricular points and the clinical application characteristics of different auricular acupuncture schools are analyzed, so as to provide useful reference for the clinical application, basic research and national standards of auricular acupuncture in the future.
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http://dx.doi.org/10.13703/j.0255-2930.20200404-k0006DOI Listing
December 2020

[Revelation and reflection of overseas acupuncture to acupuncture in China].

Zhongguo Zhen Jiu 2020 Oct;40(10):1123-7

School of Acupuncture-Moxibustion and Tuina, Hebei University of CM, Shijiazhuang 050200, China.

The development and innovation of overseas acupuncture was expounded from four aspects: meridian diagnosis and physical examination, activity of acupoint, acupuncture and moxibustion method and acupuncture level, enrichment and improvement of TCM theory and technology, and it was summarized what can be learned of principles, methods, prescriptions, acupoints and technology. On the basis of inheriting the experience of ancient acupuncture and moxibustion, the advanced experience and theory at home and abroad should be accommodated , which is helpful to better perfect and reconstruct the theoretical system of acupuncture and moxibustion, and realize the sustainable development of acupuncture and moxibustion.
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http://dx.doi.org/10.13703/j.0255-2930.20191118-k0006DOI Listing
October 2020

[Effect of moxibustion on inflammatory factors and oxidative stress factors in patients with knee osteoarthritis: a randomized controlled trial].

Zhongguo Zhen Jiu 2020 Sep;40(9):913-7

College of Acupuncture-Moxibustion and Tuina, Hebei Univerisity of TCM, Shijiazhuang 050091, China.

Objective: To compare the clinical efficacy between moxibustion and acupuncture for knee osteoarthritis (KOA), and to observe the effect on serum tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β), superoxide dismutase (SOD) and malondialdehyde (MDA).

Methods: A total of 60 patients with KOA were randomized into a moxibustion group (30 cases, 2 cases dropped off) and an acupuncture group (30 cases, 2 cases dropped off). In the aucpuncture group, acupuncture was applied at Neixiyan (EX-LE 4), Dubi (ST 35), Heding (EX-LE 2), Xuehai (SP 10), Liangqiu (ST 34), Zusanli (ST 36) and point on the affected side for 30 min.In the moxibustion group, moxibustion was adopted at knee for 60 min. The treatment was given once every two days for 4 weeks, totally 14 times. Before and after treatment, the western ontario and McMaster universities osteoarthritis index (WOMAC) score was compared, and the therapeutic effect was evaluated in the two groups. The contents of serum TNF-αand IL-1β, the activity of serum SOD and the serum level of MDA were detected in the two groups.

Results: Compared before treatment, the WOMAC scores and the contents of serum TNF-α, IL-1β and MDA after treatment were reduced (<0.05), the activity of serum SOD was increased (<0.05) in the two groups. In the moxibustion group, the WOMAC score and the contents of serum TNF-α, IL-1β and MDA after treatment were lower than the acupuncture group (<0.05), the activity of serum SOD was higher than the acupuncture group (<0.05). The total effective rate was 89.3% (25/28) in the moxibustion group, which was superior to 42.9% (12/28) in the acupuncture group (<0.05).

Conclusion: Moxibustion and acupuncture can relieve KOA symptoms, and the therapeutic effect of moxibustion is superior to acupuncture. The mechanism may be related to the reduction of serum inflammatory factor and oxidative stress factor.
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http://dx.doi.org/10.13703/j.0255-2930.20200310-k0011DOI Listing
September 2020

[Comparision of therapertic effect of different acupuncture methods for knee osteoarthritis].

Zhen Ci Yan Jiu 2020 Jul;45(7):569-73

School of Acupuncture-moxibustion and Tuina, Hebei University of Chinese Medicine, Shijiazhuang 050091, China.

Objective: To compare the clinical efficacy of acupuncture, electroacupuncture (EA) and moxibustion in the treatment of knee osteoarthritis (KOA).

Methods: A total of eight-four patients with KOA were randomly and equally divided into acupuncture group, EA group and moxibustion group. Neixiyan (EX-LE40), Dubi (ST35), Heding (EX-LE2), Liangqiu (ST34), Xuehai (SP10), Zusanli (ST36) and Ashi-point on the affected side of the body were punctured with filiform needles or EA (2 Hz/100 Hz) for 30 min. In the moxibustion group, moxibustion was applied to the surrounding area of the affected joint for 60 min. The treatment was conducted once every other day for 4 weeks. The pain degree was assessed by using numerical rating scale (NRS) and the Western Ontario McMaster Universities Osteoarthritis Index (WOMAC) scale (0-240 points) was used to evaluate the severity of KOA. The "Minimal Clinically Important Improvement (MCII)" was used to assess the therapeutic effect after the treatment.

Results: After the treatment, the scores of NRS, and the pain, stiffness, motor function and total scores of WOMAC were significantly decreased in the three groups compared with their own pre-treatment (<0.05), and were obviously lower in the EA and moxibustion groups than in the acupuncture group (<0.05), and those of the moxibustion group was notably lower than those of the acupuncture group (<0.05). Of the 28 cases in the acupuncture, EA and moxibustion groups, 11, 17 and 22 were effective, with the effective rate being 39.29%, 60.71% and 78.57%, respectively. The comprehensive therapeutic effect of the moxibustion group was significantly superior to that of the acupuncture and EA groups (<0.05).

Conclusion: All the three different kinds of acupuncture and moxibustion methods have positively regulatory effect on KOA, and moxibustion is the best for reducing the joint pain and stiffness, and improving the motor function.
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http://dx.doi.org/10.13702/j.1000-0607.191015DOI Listing
July 2020

[Clinical application characteristics of different acupuncture-moxibustion therapies for knee osteoarthritis in the past decade: an analysis based on data mining techniques].

Zhen Ci Yan Jiu 2020 Jun;45(6):490-4

Institute of Acupuncture-moxibustion and Massage of Hebei University of Traditional Chinese Medicine, Shijiazhuang 050200, China.

Objective: To investigate the application characteristics of different acupuncture-moxibusition (acu-moxi) therapies for knee osteoarthritis (KOA), and to provide a reference for clinical application.

Methods: Computer and data mining techniques were used to establish a database of acu-moxi therapies for KOA, and the articles on acu-moxi in the treatment of KOA in the past decade were collected, screened, entered, reviewed, and analyzed. Acu-moxi therapies for KOA were summarized in terms of treatment method, clinical effect, association between treatment method and acupoint selection, needle specifications, and depth of acupuncture.

Results: There are as many as 46 kinds of operation methods to treat knee osteoarthritis, among which moxibustion method has the highest frequency and the most operation methods. Traditional Chinese acupuncture and moxibustion technology has a significant effect on knee osteoarthritis, and the effective rate is more than 90%, among which the minimally invasive technique of traditional Chinese medicine has the highest cure rate and recovery rate. The relationship between traditional Chinese medicine operation and acupoint selection shows that the acupuncture and moxibustion therapy guided by traditional Chinese medicine theory pays more attention to acupoint selection based on syndrome differentiation, while the minimally invasive technique of traditional Chinese medicine is mostly proximal acupoint selection. The diameter of the needle with the highest usage frequency was 0.3 mm. The larger the diameter of the needle, the higher the specificity. The level of acupuncture was mostly tendon, followed by bone. The cure rate of acupuncture to bone is the highest.

Conclusion: Acupuncture and moxibustion has a significant effect in the treatment of KOA, with a profound theoretical basis; treatment of KOA should emphasize overall conditioning, and acupuncture should reach the bone and the tendon, so as to achieve a better clinical effect.
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http://dx.doi.org/10.13702/j.1000-0607.190360DOI Listing
June 2020

[Feasibility analysis on acupuncture therapy for the treatment of Corona Virus Disease 2019 and the exploration on the application scheme].

Zhen Ci Yan Jiu 2020 May;45(5):345-50

Institute of Acupuncture Moxibustion and Massage of Hebei University of Chinese Medicine, Shijiazhuang 050200, China.

The situation of Corona Virus Disease 2019 (COVID-19) is still severe at present. In order to better fight against the epidemic and give full play to the advantages of traditional Chinese medicine, we explored the feasibility of acupuncture therapy in the intervention of COVID-19 through analyzing the relevant literature in both ancient and modern time. Additionally, we analyzed the intervention scheme of acupuncture for COVID-19 developed by China Association of Acupuncture and Moxibustion and supplemented the protocol of the intervention with auricular acupuncture. It was proposed that the advantages of acupuncture and moxibustion should be fully displayed while Chinese herbal medications have been applied in the treatment of COVID-19. During treatment, acupuncture physicians should be rationally allocated to a certain proportion so as to adequately utilize comprehensive therapeutic approaches and guarantee people's safety to the greatest extent. Eventually, the clinical therapeutic effect may be improved, the national resources be economized on and the COVID-19 epidemic be conquered early.
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http://dx.doi.org/10.13702/j.1000-0607.200275DOI Listing
May 2020

Pharmacokinetics after Periocular Methylprednisolone Sodium Succinate Injection in Rabbit Eyes.

Evid Based Complement Alternat Med 2020 27;2020:8120398. Epub 2020 Feb 27.

Department of Ophthalmology, The Second Hospital of Jilin University, Changchun, Jilin Province 130021, China.

The present study aimed to determine the pharmacokinetics and distribution of methylprednisolone sodium succinate (MPSS) and its metabolic product methylprednisolone (MP) in plasma and ocular tissues after periocular injection of MPSS in rabbit eyes. Forty-eight healthy New Zealand white rabbits were randomly divided into 12 groups, including the control group and 11 MPSS-treated groups sampling at different time points. Rabbits in the MPSS-treated groups underwent left eye periocular injection of MPSS (10 mg). The pharmacokinetics of MPSS and MP in plasma and ocular tissues (including aqueous humor, vitreous, iris, lens, sclera, optic nerve, and choroid and retina) were investigated by liquid chromatography tandem mass spectrometry (LC-MS/MS). After periocular injection, the time of maximum concentration () of MPSS ranged from 0.25 h to 1 h in ocular tissues and was 0.25 h in plasma. of MP in ocular tissues ranged from 0.5 h to 6 h, and of MP in plasma was 0.5 h. The maximum concentration () of MPSS and MP and the area under the curve (AUC ) in ocular tissues from high to low was sclera, optic nerve, choroid and retina, iris, and lens. Especially, the concentrations of MPSS and MP in the lens were much lower when compared with the other ocular tissues. After periocular administration, MPSS could be rapidly metabolized to its active constituent MP in the ocular tissues. Also, the MPSS can be delivered effectively into the posterior segment of the eye (choroid and retina), while not easily be absorbed by the lens.
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http://dx.doi.org/10.1155/2020/8120398DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073489PMC
February 2020

[Utility of preoperative voice therapy on the voice recovery of vocal cord polyps patients undergoing the microsurgery].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2018 Mar;32(6):408-411

Department of ENT, Xiamen University Zhongshan Hospital,Xiamen.

Objectives: To evaluate the therapeutic effect on the voice recovery of patients with vocal cord polyps undergoing the microsurgery of preoperative voice therapy.

Methods: Twenty-six patients diagnosed with unilateral vocal cord polyp under stroboscope, who needed to undergo vocal cord loss resection under supportive laryngoscope, were randomly divided into control group (non-voice training) and treatment group (voice training), with each group of 13 patients. Patients in control group were just treated with surgical operation. Apart from surgical treatment, patients in treatment group received 6 hours intensive vocal therapy one week before the surgery. The therapy courses consist of the propaganda and education of voice care, postoperative vocal instruction and the patients' self-training under the guidance of voice therapists. The acoustic parameters (irregularity, breathiness, grade, jitter and shimmer) of the same patient were collected 24 to 48 hours before the surgery and 14 days after the surgery with Ling WAVES. The results were analyzed with SPSS 19.0.

Results: The differences of all the five preoperative voice parameters between control group and treatment group are not significant; but postoperative breathiness and jitter in treatment group were significantly lower than that in control group, while the differences of irregularity, overall severity and shimmer were not significant between control group and treatment group. In control group, breathiness and jitter were significantly improved after surgery, while the differences of irregularity, breathiness and shimmer were not significant between preoperation and postoperation. In treatment group, all the five voice parameters were significantly improved after surgery. According to the laryngostroboscopic examination, the vocal fold polyps were excised completely in both groups.

Conclusions: Preoperative voice therapy contributes to the recovery of voice quality of the patients with vocal cord polyps. Combined intervention of surgery and voice therapy is an effective method to treat the patients with vocal cord polyps.
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http://dx.doi.org/10.13201/j.issn.1001-1781.2018.06.003DOI Listing
March 2018

[Analysis and evaluation of resourceful chemical compositions in different parts of Zanthoxylum bungeanum fruit and its seed oil].

Zhongguo Zhong Yao Za Zhi 2016 Aug;41(15):2781-2789

Gansu ShengyuanJuxiang Agriculture Development Co., Ltd., Tianshui 741000, China.

The study aims of this study is to analyze and evaluate the resourceful chemical compositions in different parts of mature fruit of Zanthoxylum bungeanum, and provide a scientific basis for the comprehensive utilization of this medicinal plant resources. GC-MS method was used to analyze the volatile oils and the fatty acids, and HPLC method was used to determine the flavonoids and phenolic acids in the pericarp, seed and seed oil of Z.bungeanum. There were 26, 19 and 11 kinds of volatile components detected in the pericarp, seed and seed oil, respectively, in which terpenoids and their oxy-derivatives were the main components, and the contents of linalool and its esters in pericarp were relatively high. The contents of total fatty acids in the pericarp, seed and seed oil were 108.42, 331.63, 966.04 mg•g⁻¹, respectively.Oleic acid, linoleic acid andα-linolenic acid were abundantin all samples. The pericarp contains relatively high content of flavonoids, such as hyperoside, quercitrin, rutin, isoquercitrin, while the above components were not detected in the seed and seed oil. These results confirmed that the fruit of Z.bungeanum contains high contents of the resourceful chemical compositions, and their composition and contents were differed among organs, which provide a scientific basis for the utilization of Z.bungeanumfruit.
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http://dx.doi.org/10.4268/cjcmm20161506DOI Listing
August 2016

The Sequence Characteristics and Expression Models Reveal Superoxide Dismutase Involved in Cold Response and Fruiting Body Development in Volvariella volvacea.

Int J Mol Sci 2016 Jan 14;17(1). Epub 2016 Jan 14.

Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

As the first defence for cells to counteract the toxicity of active oxygen, superoxide dismutase (SOD) plays an important role in the response of living organisms to stress and cell differentiation. One extracellular Cu-ZnSOD (ecCu-ZnSOD), and two MnSODs, were identified based on the Volvariella volvacea genome sequence. All three genes have complicated alternative splicing modes during transcription; only when the fourth intron is retained can the Vv_Cu-Znsod1 gene be translated into a protein sequence with SOD functional domains. The expression levels of the three sod genes in the pilei are higher than in the stipe. The Vv_Cu-Znsod1 and the Vv_Mnsod2 are co-expressed in different developmental stages of the fruiting body, with the highest level of expression in the pilei of the egg stage, and they show a significant, positive correlation with the efficiency of karyogamy, indicating the potential role of these two genes during karyogamy. The expression of the ecCu-Znsod and two Vv_Mnsod genes showed a significant up-regulated when treated by cold stress for one hour; however, the lack of the intracellular Cu-ZnSOD encoding gene (icCu-Znsod) and the special locus of the ecCu-Znsod gene initiation codon suggested a possible reason for the autolysis phenomenon of V. volvacea in cold conditions.
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http://dx.doi.org/10.3390/ijms17010034DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4730280PMC
January 2016

Bioaggregate of photo-fermentative bacteria for enhancing continuous hydrogen production in a sequencing batch photobioreactor.

Sci Rep 2015 Nov 5;5:16174. Epub 2015 Nov 5.

State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.

Hydrogen recovery through solar-driven biomass conversion by photo-fermentative bacteria (PFB) has been regarded as a promising way for sustainable energy production. However, a considerable fraction of organic substrate was consumed for the growth of PFB as biocatalysts, furthermore, these PFB were continuously washed out from the photobioreactor in continuous operation because of their poor flocculation. In this work, PFB bioaggregate induced by L-cysteine was applied in a sequencing batch photobioreactor to enhance continuous hydrogen production and reduce biomass washout. The effects of the hydraulic retention time (HRT), influent concentration and light intensity on hydrogen production of the photobioreactor were investigated. The maximum hydrogen yield (3.35 mol H2/mol acetate) and production rate (1044 ml/l/d) were obtained at the HRT of 96 h, influent concentration of 3.84 g COD/l, and light intensity of 200 W/m(2). With excellent settling ability, biomass accumulated in the photobioreactor and reached 2.15 g/l under the optimum conditions. Structural analysis of bioaggregate showed that bacterial cells were covered and tightly linked together by extracellular polymeric substances, and formed a stable structure. Therefore, PFB bioaggregate induced by L-cysteine is an efficient strategy to improve biomass retention capacity of the photobioreactor and enhance hydrogen recovery efficiency from organic wastes.
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http://dx.doi.org/10.1038/srep16174DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4633638PMC
November 2015

Simultaneous hydrogen and ethanol production from cascade utilization of mono-substrate in integrated dark and photo-fermentative reactor.

Biotechnol Biofuels 2015 22;8(1). Epub 2015 Jan 22.

State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150090 China.

Background: Integrating hydrogen-producing bacteria with complementary capabilities, dark-fermentative bacteria (DFB) and photo-fermentative bacteria (PFB), is a promising way to completely recover bioenergy from waste biomass. However, the current coupled models always suffer from complicated pretreatment of the effluent from dark-fermentation or imbalance between dark and photo-fermentation, respectively. In this work, an integrated dark and photo-fermentative reactor (IDPFR) was developed to completely convert an organic substrate into bioenergy.

Results: In the IDPFR, Ethanoligenens harbinese B49 and Rhodopseudomonas faecalis RLD-53 were separated by a membrane into dark and photo chambers, while the acetate produced by E. harbinese B49 in the dark chamber could freely pass through the membrane into the photo chamber and serve as a carbon source for R. faecalis RLD-53. The hydrogen yield increased with increasing working volume of the photo chamber, and reached 3.38 mol H2/mol glucose at the dark-to-photo chamber ratio of 1:4. Hydrogen production by the IDPFR was also significantly affected by phosphate buffer concentration, glucose concentration, and ratio of dark-photo bacteria. The maximum hydrogen yield (4.96 mol H2/mol glucose) was obtained at a phosphate buffer concentration of 20 mmol/L, a glucose concentration of 8 g/L, and a ratio of dark to photo bacteria of 1:20. As the glucose and acetate were used up by E. harbinese B49 and R. faecalis RLD-53, ethanol produced by E. harbinese B49 was the sole end-product in the effluent from the IDPFR, and the ethanol concentration was 36.53 mmol/L with an ethanol yield of 0.82 mol ethanol/mol glucose.

Conclusions: The results indicated that the IDPFR not only circumvented complex pretreatments on the effluent in the two-stage process, but also overcame the imbalance of growth and metabolic rate between DFB and PFB in the co-culture process, and effectively enhanced cooperation between E. harbinense B49 and R. faecalis RLD-53. Moreover, simultaneous hydrogen and ethanol production were achieved by coupling E. harbinese B49 and R. faecalis RLD-53 in the IDPFR. According to stoichiometry, the hydrogen and ethanol production efficiencies were 82.67% and 82.19%, respectively. Therefore, IDPFR was an effective strategy for coupling DFB and PFB to fulfill efficient energy recovery from waste biomass.
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http://dx.doi.org/10.1186/s13068-014-0191-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4308915PMC
February 2015

Cytotoxic activity of some Asarum plants.

Fitoterapia 2008 Jun 9;79(4):293-7. Epub 2008 Feb 9.

The State Key Laboratory of Natural and Biominetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, P.R. China.

The cytotoxic activity against some tumor cell lines of 16 commonly used species of Asarum was evaluated in this study. All of these plants were widely used in Asian countries as traditional medicines or folk medicines. Their inhibitory activities against four tumor cell lines (HL-60, BGC-823, KB and Bel-7402) were compared. It was observed that 10 of the tested extracts (eight ethanol extracts and two water extracts) among 32 extracts of these plants showed cytotoxic activity. Those 95% ethanol extractions from A. caudigerellum, A. forbesii, A. inflatum and A. maximum exhibited the highest cytotoxic activity, and 95% ethanol extracts or water extracts of A. sieboldii var. seoulense, A. himalaicum, A. splendens and A. crispulatum showed selective activity against one or two cells among the tested tumor cells. This is the first report of Asarum plants possessing cytotoxic activity against tumor cell lines.
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http://dx.doi.org/10.1016/j.fitote.2008.02.002DOI Listing
June 2008

Cardenolides from Saussurea stella with cytotoxicity toward cancer cells.

J Nat Prod 2007 Sep 11;70(9):1429-33. Epub 2007 Sep 11.

The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing, People's Republic of China.

Three new cardenolides, 3-O-beta-D-fucopyranosylstrophanthidin (1), 3-O-beta-D-quinovopyranosylperiplogenin (2), and 3-O-beta-D-glucopyranosyl-(1 --> 4)-alpha- l-rhamnopyranosylcannogenin (3), together with seven known cardenolides (4- 10), were isolated from a cytotoxic ethanol extract of the whole dried plants of Saussurea stella. The structures of these compounds were established by spectroscopic and chemical methods. When the cytotoxicity of compounds 2- 10 toward Bel-7402 human hepatoma cells and BGC-823 human gastric cancer cells was evaluated, all compounds showed IC 50 values of <1 microM for both cell lines. This is the first report of cardenolides occurring in a species of the family Asteraceae.
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http://dx.doi.org/10.1021/np070150oDOI Listing
September 2007

[Inhibitory effects of 11 coumarin compounds against growth of human bladder carcinoma cell line E-J in vitro].

Zhong Xi Yi Jie He Xue Bao 2007 Jan;5(1):56-60

The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Science, Peking University, Beijing 100083, China.

Objective: To screen antitumor active compounds, drug-like or leading compounds from Chinese traditional and herbal drugs.

Methods: Eleven coumarin compounds isolated from the Chinese traditional and herbal drugs were studied for their antitumor activities in vitro by determining the inhibition rates against growth of human bladder carcinoma cell line E-J.

Results: It showed that umbelliferone, scoparone, demethylfuropinarine, isopimpinellin, forbesoside, columbianadin, decursin and glycycoumarin inhibited the growth of human bladder carcinoma cell line E-J in vitro and their activities showed a concentration-effect relationship. The inhibitory effects of forbesoside, columbianadin, decursin and umbelliferone, with IC50 values of 7.50x10(-7), 2.30x10(-6), 6.00x10(-6) and 1.30x10(-6) mol/L, respectively, were stronger than those of the other tested compounds. However, xanthotoxin, esculin and sphondin did not inhibit the growth of human bladder carcinoma cell line E-J in this assay condition.

Conclusion: These findings indicate that forbesoside, columbianadin, esculin, decursin and umbelliferone would be effective or regarded as potent drug-like or leading compounds against human bladder carcinoma.
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http://dx.doi.org/10.3736/jcim20070111DOI Listing
January 2007

Triterpene saponins from the leaves of Ilex kudingcha.

J Nat Prod 2005 Aug;68(8):1169-74

School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100083, People's Republic of China.

Nine new triterpene saponins, ilekudinosides K-S (1-9), and eight known triterpene saponins were isolated from the 70% ethanol extract of the leaves of Ilex kudingcha. The new saponins were characterized as 3-O-alpha-L-rhamnopyranosyl(1-->2)-beta-D-glucopyranosyl-alpha-kudinlactone (1), 3-O-beta-D-glucopyranosyl(1-->3)-alpha-L-arabinopyranosyl-beta-kudinlactone (2), 3-O-alpha-L-rhamnopyranosyl(1-->2)-alpha-L-arabinopyranosyl-gamma-kudinlactone (3), 3-O-beta-D-glucopyranosyl(1-->2)-beta-D-glucopyranosyl-alpha-kudinlactone (4), 3-O-beta-D-glucopyranosyl(1-->2)-alpha-L-arabinopyranosyl-alpha-kudinlactone (5), 3-O-beta-D-glucopyranosyl(1-->3)-alpha-L-arabinopyranosyl-alpha-kudinlactone (6), 3-O-alpha-L-rhamnopyranosyl(1-->2)-beta-D-glucopyranosyl-beta-kudinlactone (7), 3-O-alpha-L-rhamnopyranosyl(1-->2)-alpha-L-arabinopyranosyl-beta-kudinlactone (8), and 3-O-alpha-L-rhamnopyranosyl(1-->2)-beta-D-glucopyranosyl-gamma-kudinlactone (9), respectively. The structures and stereochemistry of compounds 1-9 were elucidated by spectroscopic data interpretation and chemical degradation.
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http://dx.doi.org/10.1021/np050043zDOI Listing
August 2005

The cytotoxic activity of ursolic acid derivatives.

Eur J Med Chem 2005 Jun;40(6):582-9

School of Pharmaceutical Sciences, Peking University, No. 38 Xueyuan Road, Haidian District, Beijing 100083, China.

Ursolic acid and 2alpha-hydroxyursolic acid isolated from apple peels were found to show growth inhibitory activity against four tumor cell lines, HL-60, BGC, Bel-7402 and Hela. Structural modifications were performed on the C-3, C-28 and C-11 positions of ursolic acid and the cytotoxicity of the derivatives was evaluated. The SAR revealed that the triterpenes possessing two hydrogen-bond forming groups (an H-donor and a carbonyl group) at positions 3 and 28 exhibit cytotoxic activity. The configuration at C-3 was found to be important for the activity. Introduction of an amino group increased the cytotoxicity greatly. A 3beta-amino derivative was 20 times more potent than the parent ursolic acid. The 28-aminoalkyl dimer compounds showed selective cytotoxicity.
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http://dx.doi.org/10.1016/j.ejmech.2005.01.001DOI Listing
June 2005