Publications by authors named "Rui Zhu"

608 Publications

and intracellular inhibitory activities of nosiheptide against .

Front Microbiol 2022 26;13:926361. Epub 2022 Jul 26.

National Clinical Laboratory on Tuberculosis, Beijing Key Laboratory on Drug-Resistant Tuberculosis, Beijing Chest Hospital, Capital Medical University, Beijing, China.

The high level of inherent drug resistance of makes the infection caused by it very difficult to be treated. The objective of this study was to evaluate the potential of nosiheptide (NOS) as a new drug candidate for treating infections. The microplate AlamarBlue assay was performed to determine the minimum inhibitory concentrations (MICs) of NOS for 28 reference strains of rapidly growing mycobacteria (RGM) and 77 clinical isolates of . Time-kill kinetic and post-antibiotic effect (PAE) of NOS against was evaluated. Its bactericidal activity against in macrophages was determined by an intracellular colony numerating assay. NOS manifested good activity against the reference strains of RGM and clinical isolates . The MICs of NOS against clinical isolates ranged from 0.0078 to 1 μg/ml, and the MIC and MIC were 0.125 μg/ml and 0.25 μg/ml, respectively. The pattern of growth and kill by NOS against was moderate with apparent concentration-dependent characteristics, and the PAE value of NOS was found to be ~6 h. Furthermore, NOS had low cell toxicity against the THP-1 cell line after 48 h of exposure (IC = 106.9 μM). At 4 μg/ml, NOS exhibited high intracellular bactericidal activity against reference strains with an inhibitory rate of 66.52% ± 1.51%, comparable with that of clarithromycin at 2 μg/ml. NOS showed suitable inhibitory activities against and in macrophages and could be a potential drug candidate to treat infection.
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http://dx.doi.org/10.3389/fmicb.2022.926361DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9360784PMC
July 2022

Emission Trading System, Carbon Market Efficiency, and Corporate Innovations.

Int J Environ Res Public Health 2022 Aug 5;19(15). Epub 2022 Aug 5.

Shanghai National Accounting Institute, Shanghai 201702, China.

Taking China's emission trading system (ETS) pilot in 2013 as a quasi-natural experiment, this paper uses the difference-in-differences (DID) models to study whether the regional pilot ETS can promote technological innovation in enterprises. In addition, this paper examines the influence mechanism of the ETS innovation effect, with a focus on three key dimensions of the carbon market efficiency: market price effectiveness, market product diversity, and market order normativity. The results show that the pilot ETS has significantly promoted the technological innovation of regulated enterprises, specifically, 1.405*** for the total R&D investment, and 2.783*** for the number of patent applications. Moreover, the regional carbon price has a positive moderating effect on the innovation effect of ETS. Meanwhile, the innovation effect is more significant when the gap between the carbon price and the marginal abatement cost (MAC) of CO is smaller, when the carbon financial derivatives are more abundant, or when the local market supervision is stronger. This study provides empirical evidence for the improvement of the national unified market and provides useful policy implications for developing countries to design ETS suitable for their national conditions.
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http://dx.doi.org/10.3390/ijerph19159683DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9368728PMC
August 2022

Research on the Evaluation of Moral Education Effectiveness and Student Behavior in Universities under the Environment of Big Data.

Authors:
Rui Zhu

Comput Intell Neurosci 2022 30;2022:2832661. Epub 2022 Jul 30.

Publicity Department, Shandong Management University, Jinan, Shandong 250000, China.

Traditional moral evaluation relies on artificial and subjective evaluation by teachers, and there are subjective errors or prejudices. To achieve further objective evaluation, students' classroom performance can be identified, and the effectiveness of moral education can be evaluated based on student behavior. Since student classroom behavior is random and uncertain, in order to accurately evaluate its indicators, a large amount of student classroom behavior data must be used as the basis for analysis, while certain techniques are used to filter out valuable information from it. In this paper, an improved graph convolutional network algorithm is proposed to study students' behaviors in order to further improve the accuracy of moral education evaluation in universities. The technique of video recognition is used to achieve student behavior recognition, thus helping to improve the quality of moral education evaluation in colleges and universities. First, the multi-information flow data related to nodes and skeletons are fused to improve the computing speed by reducing the number of network parameters. Second, the spatiotemporal attention module based on nonlocal operations is constructed to focus on the most action discriminative nodes and improve the recognition accuracy by reducing redundant information. Then, the spatiotemporal feature extraction module is constructed to obtain the spatiotemporal association information of the nodes of interest. Finally, the action recognition is realized by the Softmax layer. The experimental results show that the algorithm of action recognition in this paper is more accurate and can better help moral evaluation.
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http://dx.doi.org/10.1155/2022/2832661DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356784PMC
August 2022

Effect of degenerative factors on cervical spinal cord during flexion and extension: a dynamic finite element analysis.

Biomech Model Mechanobiol 2022 Aug 5. Epub 2022 Aug 5.

Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of the Ministry of Education, Orthopaedic Department of Tongji Hospital, School of Medicine, Tongji University, 389 Xincun Road, Shanghai, 200065, China.

Spinal cord injury (SCI) is a global problem that brings a heavy burden to both patients and society. Recent investigations indicated degenerative disease is taking an increasing part in SCI with the growth of the aging population. However, little insight has been gained about the effect of cervical degenerative disease on the spinal cord during dynamic activities. In this work, a dynamic fluid-structure interaction model was developed and validated to investigate the effect of anterior and posterior encroachment caused by degenerative disease on the spinal cord during normal extension and flexion. Maximum von-Mises stress and maximum principal strain were observed at the end of extension and flexion. The abnormal stress distribution caused by degenerative factors was concentrated in the descending tracts of the spinal cord. Our finding indicates that the excessive motion of the cervical spine could potentially exacerbate spinal cord injury and enlarge injury areas. Stress and strain remained low compared to extension during moderate flexion. This suggests that patients with cervical degenerative disease should avoid frequent or excessive flexion and extension which could result in motor function impairment, whereas moderate flexion is safe. Besides, encroachment caused by degenerative factors that are not significant in static imaging could also cause cord compression during normal activities.
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http://dx.doi.org/10.1007/s10237-022-01617-xDOI Listing
August 2022

Population pharmacokinetic analysis of etrolizumab in patients with moderately-to-severely active ulcerative colitis.

CPT Pharmacometrics Syst Pharmacol 2022 Jul 19. Epub 2022 Jul 19.

Genentech, Inc., South San Francisco, California, USA.

Etrolizumab is an IgG1-humanized monoclonal antibody that specifically targets the β7 subunit of α4β7 and α4Eβ7 integrins, and it has been evaluated for the treatment of moderately-to-severely active ulcerative colitis (UC). Population pharmacokinetic (PK) analysis was performed to characterize etrolizumab PK properties in patients with moderately-to-severely active UC and evaluate covariate impacts on exposure. The population PK model was developed based on etrolizumab serum concentrations from patients with moderately-to-severely active UC enrolled in six studies (one phase I, one phase II, and four phase III) and validated using another phase III clinical trial. Stepwise covariate modeling was used to evaluate the impact of 23 prespecified covariates. Etrolizumab PK was best described by a two-compartment model with first-order absorption, with clearance decreasing over time. Population typical values were 0.260 L/day for clearance (CL) during the first dosing internal, 2.61 L for central volume, 71.2% for bioavailability, and 0.193/day for absorption rate. CL reduced over the study duration, the typical maximum reduction was 26% with an onset half-life of 4.8 weeks. Consequently, the predicted mean terminal half-life was shorter after a single dose (13.0 days) compared to that at steady-state (17.1 days). Baseline body weight and albumin were the most impactful covariates for etrolizumab exposure. Final population PK model well characterized the PK properties of etrolizumab in patients with moderately-to-severely active UC and identified influential covariate effects.
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http://dx.doi.org/10.1002/psp4.12846DOI Listing
July 2022

Machine learning for distinguishing right from left premature ventricular contraction origin using surface electrocardiogram features.

Heart Rhythm 2022 Jul 14. Epub 2022 Jul 14.

Section of Pacing and Electrophysiology, Division of Cardiology, the First Affiliated Hospital with Nanjing Medical University, Nanjing, China. Electronic address:

Background: Precise localization of the site of origin of premature ventricular contractions (PVCs) before ablation can facilitate the planning and execution of the electrophysiological procedure.

Objective: The purpose of this study was to develop a predictive model that can be used to differentiate PVCs between the left ventricular outflow tract and right ventricular outflow tract (RVOT) using surface electrocardiogram characteristics.

Methods: A total of 851 patients undergoing radiofrequency ablation of premature ventricular beats from January 2015 to March 2022 were enrolled. Ninety-two patients were excluded. The other 759 patients were enrolled into the development (n = 605), external validation (n = 104), or prospective cohort (n = 50). The development cohort consisted of the training group (n = 423) and the internal validation group (n = 182). Machine learning algorithms were used to construct predictive models for the origin of PVCs using body surface electrocardiogram features.

Results: In the development cohort, the Random Forest model showed a maximum receiver operating characteristic curve area of 0.96. In the external validation cohort, the Random Forest model surpasses 4 reported algorithms in predicting performance (accuracy 94.23%; sensitivity 97.10%; specificity 88.57%). In the prospective cohort, the Random Forest model showed good performance (accuracy 94.00%; sensitivity 85.71%; specificity 97.22%).

Conclusion: Random Forest algorithm has improved the accuracy of distinguishing the origin of PVCs, which surpasses 4 previous standards, and would be used to identify the origin of PVCs before the interventional procedure.
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http://dx.doi.org/10.1016/j.hrthm.2022.07.010DOI Listing
July 2022

Molecular Dynamics Simulation on the Wettability of Nanoscale Wrinkles: High Water Adhesion of Rose Petals.

Langmuir 2022 Jul 14;38(29):8854-8861. Epub 2022 Jul 14.

Shanghai YangZhi Rehabilitation Hospital (Shanghai Sunshine Rehabilitation Center), School of Medicine, Tongji University, Shanghai 200092, China.

Understanding the high water adhesion of rose petals is of great significance in artificial surface design. With all-atom molecular dynamics simulation, the wettability of nanoscale wrinkles was explored and compared to that of nanoscale strips with favorable hydrophobicity. The dewetting and wetting of gaps between nanoscale structures represent the Cassie-Baxter (CB) and Wenzel (WZ) states of the macroscopic droplet deposited on the textured surface, respectively. We uncovered the intermediate state, which is different from the CB and WZ states for wrinkles. Structures and free-energy profiles of metastable and transition states under various pressures were also investigated. Moreover, free-energy barriers for the (de)wetting transitions were quantified. On this basis, the roles of pressure and the unique structures of nanoscale wrinkles in the high water adhesion of rose petals were identified.
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http://dx.doi.org/10.1021/acs.langmuir.2c00974DOI Listing
July 2022

Induced morphology orientation of α-FeOOH by kaolinite for enhancing peroxymonosulfate activation.

J Colloid Interface Sci 2022 Jun 30;626:494-505. Epub 2022 Jun 30.

School of Chemical and Environmental Engineering, China University of Mining and Technology (Beijing), Beijing 100083, P.R. China. Electronic address:

Persulfate activation technology based on sulfate radicals is currently a hot spot in the field of environmental governance. In our work, α-FeOOH was successful in situ loaded on kaolinite surface through a simple one-step hydrothermal process. The prepared composites were systematically characterized, and the relationship between the structural properties and peroxymonosulfate activation properties was explored. Interestingly, compared to bare α-FeOOH, the introduction of kaolinite in composite induced the transformation of α-FeOOH crystal and affected the morphology, where uniformly dispersed nanoparticles rather than rod-like agglomerated crystals appeared. The received FeOOH/kaolinite composite exhibited admirable adsorption and degradation of ciprofloxacin performance with the removal efficiency of 86.1%, and the degradation rate constant was up to 5.2 times higher than that of bare α-FeOOH. In addition, the main active species in the catalytic oxidation system are surface-bound SO, OH and free O. This work would give a deep insight into the role of natural minerals in composite catalytic materials and the construction of high-efficient mineral-based composite materials.
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http://dx.doi.org/10.1016/j.jcis.2022.06.151DOI Listing
June 2022

Identification of Ferroptotic Genes in Spinal Cord Injury at Different Time Points: Bioinformatics and Experimental Validation.

Mol Neurobiol 2022 Jul 7. Epub 2022 Jul 7.

Department of Orthopedics, The First Affiliated Hospital of Anhui Medical University, Anhui Medical University, 218 Jixi Road, Hefei, 230022, China.

Programmed cell death (PCD) is an important pathologic process after spinal cord injury (SCI). As a new type of PCD, ferroptosis is involved in the secondary SCI. However, the underlying molecular mechanism remains unclear. In this study, we validated ferroptotic phenotype in an animal model of SCI. Then, the bioinformatic analyses performed on a microarray data of SCI (GSE45006). KEGG analysis suggested that the pathways of mTOR, HIF-1, VEGF, and protein process in endoplasmic reticulum were involved in SCI-induced ferroptosis. GO analysis revealed that oxidative stress, amide metabolic process, cation transport, and cytokine production were essential biological processes in ferroptosis after SCI. We highlighted five genes including ATF-3, XBP-1, HMOX-1, DDIT-3, and CHAC-1 as ferroptotic key gene in SCI. These results contribute to exploring the ferroptotic mechanism underlying the secondary SCI and providing potential targets for clinical treatment.
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http://dx.doi.org/10.1007/s12035-022-02935-yDOI Listing
July 2022

Exposure-response relationships of etrolizumab in patients with moderately-to-severely active ulcerative colitis.

CPT Pharmacometrics Syst Pharmacol 2022 Jul 4. Epub 2022 Jul 4.

Genentech, Inc., South San Francisco, California, USA.

Etrolizumab is an IgG1-humanized monoclonal anti-β7 integrin antibody. Phase III trials with induction and/or maintenance phases were conducted in patients with moderately-to-severely active ulcerative colitis (UC) who were either previously treated with tumor necrosis factor (TNF) inhibitors (HICKORY) or were TNF inhibitor naïve (HIBISCUS I/II, LAUREL, and GARDENIA). A total of eight exposure-response analyses were conducted for two clinical outcomes (remission and endoscopic improvement) at the end of induction for studies HIBISCUS I/II (combined) and HICKORY and at the end of maintenance for studies HICKORY and LAUREL. Trough concentration at week 4 (C ) of induction was selected as the exposure metric. Exposure-response (ER) modeling was conducted using logistic regression. A full covariate model was used to examine the impact of covariates on clinical outcomes. Linear models with a single intercept for placebo and active treatments adequately described the data for all eight analyses. The etrolizumab exposure-response slope was significant (p < 0.05) for seven of the eight analyses. Baseline Mayo Clinic Score (MCS) was the only statistically significant covariate that impacted induction remission and endoscopic improvement. No statistically significant covariate was identified to impact maintenance outcomes except for baseline fecal calprotectin on endoscopic improvement for LAUREL study. A statistically significant positive ER relationship was identified for most of the clinical outcomes tested, reflecting a better treatment effect in patients with UC with higher etrolizumab C of induction. Baseline MCS was the only other significant covariate impacting induction efficacy. Besides C of induction, no consistent covariate was identified to impact maintenance efficacy.
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http://dx.doi.org/10.1002/psp4.12840DOI Listing
July 2022

Multi-scale convolution neural network with residual modules for determination of drugs in human hair using surface-enhanced Raman spectroscopy with a gold nanorod film self-assembled by inverted evaporation.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Nov 4;280:121463. Epub 2022 Jun 4.

National Engineering Research Center for Agro-Ecological Big Data Analysis & Application, Anhui University, Hefei 230601, People's Republic of China. Electronic address:

Detection of illegal drug users is crucial in controlling drug-related crimes, reducing drug prevalence, and protecting human lives to ensure social stability. In this study, surface-enhanced Raman spectroscopy (SERS) and deep learning networks were employed to determine methamphetamine, ketamine, and morphine in human hair. Drugs were obtained from hair through alkaline hydrolysis and liquid-liquid extraction, and gold nanorods were employed to prepare the self-assembled film as the SERS substrate by inverted evaporation. The film showed good uniformity and excellent sensitivity, with a relative standard deviation of 15.6% and a detection limit of at least 10 M in the SERS detection of crystal violet. The spectra of methamphetamine, ketamine, and morphine at 0.05-1.0, 0.1-2.0, and 0.1-2.0 ng/mg were obtained, and the three drugs could be detected. Inception, a multi-scale feature extraction network, was combined with residual modules (Inception-ResNet) to develop the identification models of drugs, and the effect of spectral input form as a vector or matrix was explored. Inception-ResNet with input form of matrix outweighed other methods with 100.00%, 100.00%, and 99.23% accuracies in the training, validation, and prediction sets, respectively. In brief, SERS and Inception-ResNet with the spectra in matrix form provide an efficient and accurate determination of drugs in human hair, enabling the retrospective evaluation of drug use, and the method will be anticipated to detect excitant, poison, and toxic chemicals in human hair.
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http://dx.doi.org/10.1016/j.saa.2022.121463DOI Listing
November 2022

Ultrafast charge generation in a homogenous polymer domain.

Sci Rep 2022 Jun 16;12(1):10087. Epub 2022 Jun 16.

School of Science, Shandong Jianzhu University, Jinan, 250100, Shandong Province, China.

Efficient charge generation contributes greatly to the high performance of organic photovoltaic devices. The mechanism of charge separation induced by heterojunction has been widely accepted. However, how and why free charge carriers can generate in homogenous polymer domains remains to be explored. In this work, the extended tight-binding SSH model, combined with the non-adiabatic molecular dynamics simulation, is used to construct the model of a polymer array in an applied electric field and simulate the evolution of an excited state. It is found that under a very weak external electric field 5.0 × 10 V/Å, the excited state can evolve directly into spatially separated free charges at the femtosecond scale, and the efficiency is up to 97%. The stacking structure of the polymer array leads to intermolecular electron mutualization and forms intermolecular coupling. This interaction tends to delocalize the excited states in organic semiconductors, competing with the localization caused by electron-phonon coupling. Excitons within the homogenous polymer domains have lower binding energy, less energy dissipation, and ultrafast charge separation. Therefore, the initial excited state can evolve directly into free carriers under a very weak electric field. This finding provides a reasonable explanation for ultrafast charge generation in pure polymer phases and is consistent with the fact that delocalization always coexists with ultrafast charge generation. Moreover, the devices based on homogenous polymer domains are supposed to be stress-sensitive and performance-anisotropic since the above two interactions have contrary effects and work in perpendicular directions. This work is expected to bring inspiration for the design of organic functional materials and devices.
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http://dx.doi.org/10.1038/s41598-022-13886-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9203523PMC
June 2022

HA gene amino acid mutations contribute to antigenic variation and immune escape of H9N2 influenza virus.

Vet Res 2022 Jun 15;53(1):43. Epub 2022 Jun 15.

College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, Jiangsu, China.

Based on differences in the amino acid sequence of the protein haemagglutinin (HA), the H9N2 avian influenza virus (H9N2 virus) has been clustered into multiple lineages, and its rapidly ongoing evolution increases the difficulties faced by prevention and control programs. The HA protein, a major antigenic protein, and the amino acid mutations that alter viral antigenicity in particular have always been of interest. Likewise, it has been well documented that some amino acid mutations in HA alter viral antigenicity in the H9N2 virus, but little has been reported regarding how these antibody escape mutations affect antigenic variation. In this study, we were able to identify 15 HA mutations that were potentially relevant to viral antigenic drift, and we also found that a key amino acid mutation, A180V, at position 180 in HA (the numbering for mature H9 HA), the only site of the receptor binding sites that is not conserved, was directly responsible for viral antigenic variation. Moreover, the recombinant virus with alanine to valine substitution at position 180 in HA in the SH/F/98 backbone (rF/HA virus) showed poor cross-reactivity to immune sera from animals immunized with the SH/F/98 (F/98, A180), SD/SS/94 (A180), JS/Y618/12 (T180), and rF/HA (V180) viruses by microneutralization (MN) assay. The A180V substitution in the parent virus caused a significant decrease in cross-MN titres by enhancing the receptor binding activity, but it did not physically prevent antibody (Ab) binding. The strong receptor binding avidity prevented viral release from cells. Moreover, the A180V substitution promoted H9N2 virus escape from an in vitro pAb-neutralizing reaction, which also slightly affected the cross-protection in vivo. Our results suggest that the A180V mutation with a strong receptor binding avidity contributed to the low reactors in MN/HI assays and slightly affected vaccine efficacy but was not directly responsible for immune escape, which suggested that the A180V mutation might play a key role in the process of the adaptive evolution of H9N2 virus.
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http://dx.doi.org/10.1186/s13567-022-01058-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202205PMC
June 2022

Designing Multifunctional Donor-Acceptor-Type Molecules to Passivate Surface Defects Efficiently and Enhance Charge Transfer of CsPbIBr Perovskite for High Power Conversion Efficiency.

Inorg Chem 2022 Jun 13;61(25):9469-9479. Epub 2022 Jun 13.

Key Laboratory for Special Functional Materials of Ministry of Education, and School of Materials, Center for Topological Functional Materials, and School of Physics and Electronic, Henan University, Kaifeng 475001, China.

High-density and multitype surface defects of CsPbIBr perovskite induce charge recombination and accumulation, hindering its device efficiency and stability. However, the surface defect types of CsPbIBr perovskite are still unclear, and conventional organic molecules only passivate one specific defect and cannot achieve good overall passivation. Here, density functional theory is used to explore surface defect types and properties of CsPbIBr with calculating the defect formation energy and electronic structure. Results show that the dominant deep-level defects are cationic defects (Pb) under Br-poor conditions and anionic defects (I and Br) under moderate and Br-rich conditions, originating from Pb-Pb bonding and I-I bonding. Multifunctional organic molecules containing donor and acceptor groups are used to passivate both cationic and anionic defects simultaneously. It turns out that the deep-level defects are effectively decreased by forming strong interaction of N-Pb, O-Pb, and halide-Pb bonds. Moreover, the electron and hole transfers from perovskite to molecules increase dramatically to -9.06 × 10 and 2.60 × 10 /cm and maybe improve the efficiency of power conversion. Our findings not only reveal the surface defect properties of CsPbIBr, but also offer an approach for designing new multifunctional passivators for perovskite solar cells with high conversion efficiency.
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http://dx.doi.org/10.1021/acs.inorgchem.2c00394DOI Listing
June 2022

Deferasirox shows inhibition activity against cervical cancer in vitro and in vivo.

Gynecol Oncol 2022 Jul 7;166(1):126-137. Epub 2022 Jun 7.

Laboratory of Molecular Iron Metabolism, Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, Ministry of Education Key Laboratory of Molecular and Cellular Biology, College of Life Science, Hebei Normal University, Shijiazhuang 050024, PR China. Electronic address:

Objective: Iron depletion may be a novel therapeutic strategy for cancer. This study aimed to assess the inhibition effects of deferasirox (DFX), an oral iron chelator, on cervical cancer.

Methods: In this study, we performed immunohistochemical analysis, enzyme-linked immunoassay, cell viability and invasive ability assay, cell cycle and apoptosis analysis, protein expression investigation, molecular mechanism investigation, and in vivo murine xenograft model to evaluate the impact of DFX on cervical cancer.

Results: The cervical cancer cell lines viability decreased and cell apoptosis was induced after DFX incubation. Additionally, DFX promoted cell cycle arrest by regulating the expression of cell cycle regulators cyclin D1, cyclin E and proliferating cell nuclear antigen (PCNA) in cervical cancer cell lines. DFX also decreased cell invasion by upregulating the expression of NDRG1 and downregulating c-Myc. The activation of Akt and the MEK/ERK signaling pathway was inhibited by DFX. DFX also significantly suppressed xenograft tumor growth, decreased the levels of ferritin in serum and tumor tissue, reduced iron deposits and reactive oxygen species (ROS) levels in xenografts of DFX-treated group compared with the control group, with no serious side effects.

Conclusion: Present study demonstrated the inhibitory effect of DFX against cervical cancer, and provided a potential therapeutic agent for cervical cancer.
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http://dx.doi.org/10.1016/j.ygyno.2022.05.006DOI Listing
July 2022

The Long-Term Effect of COVID-19 Disease Severity on Risk of Diabetes Incidence and the Near 1-Year Follow-Up Outcomes among Postdischarge Patients in Wuhan.

J Clin Med 2022 May 30;11(11). Epub 2022 May 30.

Department of Occupational and Environmental Health, Key Laboratory of Environment and Health, Ministry of Education & State Environmental Protection Key Laboratory of Health Effects of Environmental Pollution, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology (HUST), Wuhan 430030, China.

We assessed the nearly 1-year health consequences following discharge and related risk factors of COVID-19 infection and further explored the long-term effect of COVID-19 disease severity on the risk of diabetes incidence. This prospective study included 248 COVID-19 patients discharged from Wuhan Hospital of Traditional Chinese Medicine who were followed up between 1 March and 10 June 2021. Logistic regression models were used to evaluate risk factors. The top ten symptoms were shortness of breath (30.3%), sore or dry throat (25.7%), cough (23.2%), expectoration (23.2%), body pain (22.3%), chest tightness (20.8%), palpitations (17.8%), sleep difficulties (17.0%), fatigue (16.6%), and anxiety (15.3%). Hypertension was associated with fatigue (OR = 2.51, 95% CI: 1.08, 5.80), shortness of breath (OR = 2.34, 95% CI: 1.16, 4.69), palpitations (OR = 2.82, 95% CI: 1.26, 6.31), expectoration (OR = 2.08, 95% CI: 1.01, 4.30), and sore or dry throat (OR = 2.71, 95% CI: 1.30, 5.65). Diabetes was associated with palpitations (OR = 3.22, 95% CI: 1.18, 8.81). Critical illness was associated with an increased risk of diabetes incidence after discharge (OR = 2.90, 95% CI: 1.07, 7.88), which seemed more evident in males. Long COVID-19 symptoms were common at 1-year postdischarge; hypertension and diabetes could be projected as potential risk factors. We are among the first researchers to find that critical illness is associated with incident diabetes after discharge.
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http://dx.doi.org/10.3390/jcm11113094DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9181214PMC
May 2022

Associations of urinary polycyclic aromatic hydrocarbon metabolites and blood pressure with the mediating role of cytokines: A panel study among children.

Environ Sci Pollut Res Int 2022 Jun 1. Epub 2022 Jun 1.

Department of Occupational and Environmental Health, Ministry of Education Key Laboratory of Environment and Health, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Rd, Wuhan, 430030, Hubei, China.

Little was known regarding the relations of polycyclic aromatic hydrocarbon (PAH) mixture with children's blood pressure (BP) and its potential mechanism. We conducted a panel study with up to 3 visits across 3 seasons in 2017-2018 among 103 children aged 4-13 years. Urinary PAH metabolites (OH-PAHs) were measured by gas chromatograph-tandem triple quadrupole mass spectrometer, and serum cytokines were detected by Bio-Rad 48-Plex Screening Panel. We employed linear mixed-effects models to assess the relations of each urinary OH-PAH with BP, least absolute shrinkage and selection operator (LASSO), and weighted quantile sum (WQS) regression to evaluate associations of OH-PAHs mixture with BP, and mediation analyses for the role of serum cytokines. We found the consistently positive associations of 1-hydroxynaphthalene and 9-hydroxyphenanthrene (9-OHPh) with systolic BP (SBP), 4-OHPh, and 9-OHPh with diastolic BP (DBP) and mean arterial pressure (MAP) in a dose-responsive manner. For instance, each 1-fold increment of 9-OHPh was related with increase of 0.92% (95% confidence interval (CI): 0.25%, 1.60%) in SBP, 1.32% (95%CI: 0.25%, 2.39%) in DBP, and 1.15% (95%CI: 0.40%, 1.88%) in MAP. Meanwhile, based on LASSO and WQS regression, OH-PAHs mixture was linked with increased DBP and MAP, to which 9-OHPh and 4-OHPh were the major contributors. Such relationships were modified by passive smoking status and 3-4 times stronger in passive smokers than non-passive smokers. A 1-fold increase in 9-OHPh was associated with an elevation of 3.51% in SBP among passive smokers while that of 0.55% in SBP among non-passive smokers. Furthermore, 4-OHPh and 9-OHPh were related to multiple cytokines elevation, of which platelet-derived growth factor (PDGF) mediated 9.99% and 12.57% in 4-OHPh-related DBP and MAP elevation, respectively. Accordingly, urinary OH-PAHs dominated by 9-OHPh and 4-OHPh were dose-responsively associated with elevated BP whereby a mechanism partly involving PDGF among children.
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http://dx.doi.org/10.1007/s11356-022-21062-8DOI Listing
June 2022

Effects of inhaled JAK inhibitor GDC-4379 on exhaled nitric oxide and peripheral biomarkers of inflammation.

Pulm Pharmacol Ther 2022 Aug 26;75:102133. Epub 2022 May 26.

Medical Research Institute of New Zealand, Level 7 CSB Building Wellington Hospital, Riddiford Street, Newtown, Wellington, 6021, New Zealand. Electronic address:

Background: Janus Kinases (JAKs) mediate activity of many asthma-relevant cytokines. GDC-0214, an inhaled small molecule JAK1 inhibitor, has previously been shown to reduce fractional exhaled nitric oxide (FeNO) in patients with mild asthma, but required an excessive number of inhalations.

Aim: To assess whether GDC-4379, a new inhaled JAK inhibitor, reduces FeNO and peripheral biomarkers of inflammation.

Methods: This study assessed the activity of GDC-4379 in a double-blind, randomized, placebo-controlled, Phase 1 study in patients with mild asthma. Participants included adults (18-65y) with a diagnosis of asthma for ≥6 months, forced expiratory volume in 1 s (FEV)> 70% predicted, FeNO >40 ppb, using as-needed short-acting beta-agonist medication only. Four sequential, 14-day, ascending-dose cohorts (10 mg QD, 30 mg QD, 40 mg BID, and 80 mg QD) of 12 participants each were randomized 2:1 to GDC-4379 or placebo. The primary activity outcome was percent change from baseline (CFB) in FeNO to Day 14 compared to the pooled placebo group. Safety, tolerability, pharmacokinetics, and pharmacodynamic biomarkers, including blood eosinophils, serum CCL17, and serum CCL18, were also assessed.

Results: Of 48 enrolled participants, the mean age was 25 years and 54% were female. Median (range) FeNO at baseline was 79 (41-222) ppb. GDC-4379 treatment led to dose-dependent reductions in FeNO. Compared to placebo, mean (95% CI) percent CFB in FeNO to Day 14 was: -6 (-43, 32) at 10 mg QD, -26 (-53, 2) at 30 mg QD, -55 (-78, -32) at 40 mg BID and -52 (-72, -32) at 80 mg QD. Dose-dependent reductions in blood eosinophils and serum CCL17 were also observed. Higher plasma drug concentrations corresponded with greater FeNO reductions. No serious AEs occurred. The majority of AEs were mild to moderate. The most common AEs were headache and oropharyngeal pain. Minor changes in neutrophils were noted at 80 mg QD, but were not considered clinically meaningful.

Conclusions: In patients with mild asthma, 14-day treatment with GDC-4379 reduced FeNO levels and peripheral biomarkers of inflammation. Treatment was well tolerated without any major safety concerns.

Australian New Zealand Clinical Trials Registry: ACTRN12619000227190.
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http://dx.doi.org/10.1016/j.pupt.2022.102133DOI Listing
August 2022

[Research Progress in Delayed Bleeding after Endoscopic Submucosal Dissection of Early-Stage Gastrointestinal Cancer].

Sichuan Da Xue Xue Bao Yi Xue Ban 2022 May;53(3):381-385

Department of Gastroenterology, Fifth Medical Center, Chinese PLA General Hospital, Beijing 100853, China.

Endoscopic submucosal dissection (ESD) has been widely used in the clinical treatment of early-stage and precancerous lesions of the digestive tract. Compared with traditional open surgery, the procedure has a number of advantages, including low postprocedural recurrence rate, the location and scope of lesions not posing much restrictions on the procedure, and quick patients recovery afterwards. The procedure has hence become one of the minimally-invasive procedures commonly performed with gastrointestinal endoscope. However, due to the influence of various factors, complications such as intraoperative and postoperative bleeding, perforation, electrocoagulation syndrome and lumen stenosis may occur. Delayed postoperative bleeding, in particular, may induce cardiovascular and other related diseases due to the insidious nature of its onset, resulting in serious consequences. It is critically important for the further development of ESD that we should acquire thorough understanding and mastery of the relevant influencing factors and preventive measures of delayed bleeding after ESD of early-stage gastrointestinal cancer. We herein summarized and discussed the latest research findings in the preventative and treatment measures.
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http://dx.doi.org/10.12182/20220560503DOI Listing
May 2022

A flexible tactile sensor that uses polyimide/graphene oxide nanofiber as dielectric membrane for vertical and lateral force detection.

Nanotechnology 2022 Jul 14;33(40). Epub 2022 Jul 14.

Department of Mechanical Engineering, University of California, Berkeley, CA 94720, United States of America.

Flexible force sensors are of great interest in the fields of healthcare, physiological signals, and aircraft smart skin applications because of their compatibility with curved surfaces. However, the simultaneous detection of multidirectional forces remains an engineering challenge, despite the great progress made in recent years. Herein, we present the development of a flexible capacitive force sensor capable of efficiently distinguishing normal and sliding shear forces. A two-layer electrospun polyimide/graphene oxide (PI/GO) nanofiber membrane is used as the dielectric layer, which is sandwiched between one top electrode and four symmetrically distributed bottom electrodes. This composite membrane has an improved dielectric constant, a reduced friction coefficient, and good compressibility, leading to superior performance that includes high sensitivity over a wide operational range with measured results of 3 MPafor 0-242 kPa (0-2.2 N) and 0.92 MPafor 242-550 kPa (2.2-5 N) in the normal direction; and better than 1 Nfor 0-3 N in the- and-axis directions. The system also has a low detection limit of 10 Pa, fast response and recovery times of 39 ms and 13 ms, respectively, a good cyclic stability of 10,000 cycles at a pressure of 176 kPa, and promising potential for use in high-temperature environments (200 °C). Moreover, a prototype 4 × 4 sensor array has been fabricated and successfully used in a robotic system to grasp objects and operate a wireless toy car. As such, the proposed system could offer superior capabilities in simultaneous multidirectional force sensing for applications such as intelligent robots, human-machine interaction, and smart skin.
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http://dx.doi.org/10.1088/1361-6528/ac73a4DOI Listing
July 2022

Defining upstream enhancing and inhibiting sequence patterns for plant peroxisome targeting signal type 1 using large-scale in silico and in vivo analyses.

Plant J 2022 07 19;111(2):567-582. Epub 2022 Jun 19.

College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China.

Peroxisomes are universal eukaryotic organelles essential to plants and animals. Most peroxisomal matrix proteins carry peroxisome targeting signal type 1 (PTS1), a C-terminal tripeptide. Studies from various kingdoms have revealed influences from sequence upstream of the tripeptide on peroxisome targeting, supporting the view that positive charges in the upstream region are the major enhancing elements. However, a systematic approach to better define the upstream elements influencing PTS1 targeting capability is needed. Here, we used protein sequences from 177 plant genomes to perform large-scale and in-depth analysis of the PTS1 domain, which includes the PTS1 tripeptide and upstream sequence elements. We identified and verified 12 low-frequency PTS1 tripeptides and revealed upstream enhancing and inhibiting sequence patterns for peroxisome targeting, which were subsequently validated in vivo. Follow-up analysis revealed that nonpolar and acidic residues have relatively strong enhancing and inhibiting effects, respectively, on peroxisome targeting. However, in contrast to the previous understanding, positive charges alone do not show the anticipated enhancing effect and that both the position and property of the residues within these patterns are important for peroxisome targeting. We further demonstrated that the three residues immediately upstream of the tripeptide are the core influencers, with a 'basic-nonpolar-basic' pattern serving as a strong and universal enhancing pattern for peroxisome targeting. These findings have significantly advanced our knowledge of the PTS1 domain in plants and likely other eukaryotic species as well. The principles and strategies employed in the present study may also be applied to deciphering auxiliary targeting signals for other organelles.
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http://dx.doi.org/10.1111/tpj.15840DOI Listing
July 2022

The mediating role of plasma microRNAs in the association of phthalates exposure with arterial stiffness: A panel study.

Environ Res 2022 09 16;212(Pt D):113469. Epub 2022 May 16.

Department of Occupational and Environmental Health, Key Laboratory of Environment & Health, Ministry of Education, And State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China. Electronic address:

Phthalates exposure has been reported to be linked with arterial stiffness. However, the biological mechanisms underlying this association remain unclear. We conducted a panel study using 338 paired urine-blood samples by repeated measurements of 123 adults across 3 seasons to assess the potential mediating role of plasma microRNAs (miRNAs) in the association of phthalates exposure with arterial stiffness. We measured 10 urinary phthalate metabolites by gas chromatography-tandem mass spectrometry (GC-MS/MS) and 5 candidate arterial stiffness-related miRNAs (miR-146a, miR-222, miR-125b, miR-126, and miR-21) in plasma by real-time PCR. Arterial stiffness parameters including brachial-ankle pulse wave velocity (baPWV) and ankle-brachial index (ABI) were determined in health examinations during each visit. Linear mixed-effect (LME) models revealed that mono-methyl phthalate (MMP), mono-iso-butyl phthalate (MiBP), mono-n-butyl phthalate (MBP), mono-n-octyl phthalate (MOP), and mono-(2-ethyl-5-carboxypentyl) phthalate (MECPP) were significantly associated with one or more of the 5 plasma miRNAs (all P < 0.05). Based on weighted quantile sum (WQS) regression, we found positive associations of phthalate metabolites mixture with miR-146a, miR-125b, and miR-222, and individual MMP and MBP were the major contributors. Additionally, miR-146a was inversely related to ABI. Mediation analysis further indicated that miR-146a mediated 31.6% and 21.3% of the relationships of MMP and MiBP with ABI, respectively. Our findings suggested that certain phthalates exposure was related to plasma miRNAs alterations in a dose-response manner and miR-146a might partly mediate phthalate-associated ABI reduction.
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http://dx.doi.org/10.1016/j.envres.2022.113469DOI Listing
September 2022

Prevalence of aPhosphatidylserine/prothrombin antibodies and association with antiphospholipid antibody profiles in patients with antiphospholipid syndrome: A systematic review and meta-analysis.

Thromb Res 2022 06 2;214:106-114. Epub 2022 May 2.

Thrombosis Research Laboratory, Department of Cardio-Thoracic-Vascular Sciences and Public Health, University of Padua, Padua, Italy; Arianna Foundation on Anticoagulation, Bologna, Italy. Electronic address:

Background: Antiphospholipid syndrome (APS) is an autoimmune disorder characterized by the presence of antiphospholipid antibodies (aPLs) and thrombotic events. The association of aPLs with thrombotic events depends on the number of positive tests. Besides the three classical tests to classify APS, phosphatidylserine/prothrombin complex autoantibodies (aPS/PT) are increasingly used to better define this condition. The aim of this systematic review was to evaluate the prevalence of aPS/PT in general and according to antiphospholipid antibody profiles in patients with APS.

Methods: A systematic search of PubMed, Web of Science, and the Cochrane Library from January 1990 to September 2021 was carried out according to PRISMA guidelines. Proportions and 95% confidence intervals (CIs) were calculated using random-effects model. Publication biases were evaluated via visualization of funnel plots along with Egger's and Begg's tests.

Results: Twenty-one articles about the prevalence of aPS/PT in 1853 patients with APS were deemed eligible and analyzed according to the inclusion criteria. Pooled prevalence of aPS/PT IgG alone, IgM alone, and IgG/M were 50.0%, 45.0%, and 65.0%, respectively. No significant publication bias was detected from funnel plots or Egger's and Begg's tests. When the prevalence of aPS/PT was calculated in homogeneous aPLs, a much higher rate of pooled prevalence of aPS/PT IgG/M in patients positive for Lupus Anticoagulant (84.5%) and in those with triple positivity (83.4%) was found.

Conclusions: These data show a high rate of aPS/PT positivity in patients with APS (especially in those positive for LAC) but further studies are needed to ascertain whether this test might be useful in the laboratory classification criteria of APS.
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http://dx.doi.org/10.1016/j.thromres.2022.04.021DOI Listing
June 2022

Engineering the Entrance of a Flavonoid Glycosyltransferase Promotes the Glycosylation of Etoposide Aglycone.

ACS Synth Biol 2022 05 6;11(5):1874-1880. Epub 2022 May 6.

Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Key Laboratory of Industrial Microbiology, Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), Hubei University of Technology, Wuhan 430068, China.

Enzyme entrances, which function as the first molecular filters, influence substrate selectivity and enzymatic activity. Because of low binding affinities, engineering enzyme entrances that recognize non-natural substrates is a major challenge for artificial biocatalyst design. Here, the entrance of flavonoid glycosyltransferase UGT78D2 was engineered to promote the recognition of the aglycone of etoposide, a chemotherapeutic agent. We found that Q258, S446, R444, and R450, the key residues surrounding the substrate entrance, specifically guide the flux of etoposide aglycone, which has a high steric hindrance, into the active site; this activity was inferred to be determined by the entrance size and hydrophobic and electrostatic interactions. Engineering the coordination of Q258 and S446 to increase the entrance size and hydrophobic interaction between UGT78D2 and etoposide aglycone increased the affinity by 10.10-fold and the conversion by 10%. The entrance-engineering strategy applied in this study can improve the design of artificial biocatalysts.
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http://dx.doi.org/10.1021/acssynbio.2c00032DOI Listing
May 2022

High-Performance Computational Recognition of Communication Signals Based on Bispectral Quadratic Feature Model.

Comput Intell Neurosci 2022 26;2022:2773492. Epub 2022 Apr 26.

School of Information Engineering, Xijing University, Xi'an, Shaanxi 710123, China.

In response to the problems in the signal identification of radiation sources during the communication process, the bispectral quadratic feature model is applied to the identification algorithm for communication signals. According to the signal eigenvalues obtained from the bispectrum of the diagonal slices in the radiation source signals, the eigenvalues of the bispectrum diagonal slices can be extended from the frequency domain to the complex plane through the chirp-z operation in this paper, and the relevant data are obtained based on the bispectrum quadratic feature model of the signals by using the separation rules corresponding to the extended Babbitt distance. The bispectral quadratic feature model method is used to establish a sparse observation model, and the communication signal processing problem can be transformed into an estimation problem of signal motion parameters through the construction of a parametric database. At the same time, the high-resolution distance of communication signals is tested, and the communication signals are estimated by using the variational inference method. Finally, practical cases are analyzed, and the results indicate that the algorithm proposed in this paper can be used to identify different types of communication signals in accordance with simulated and measured data in the processing of communication signals in various environments, which has the certain anti-interference capacity to noise, can improve the identification rate of communication signals, and has verified the effectiveness and practicality of the algorithm proposed in this paper.
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http://dx.doi.org/10.1155/2022/2773492DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9064523PMC
May 2022

Osteoimmune reaction caused by a novel silicocarnotite bioceramic promoting osteogenesis through the MAPK pathway.

Biomater Sci 2022 May 31;10(11):2877-2891. Epub 2022 May 31.

Department of Orthodontics, School and Hospital of Stomatology, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Tongji University, Shanghai 200072, China.

The host immune response to an implant is a key factor in determining the fate of bone grafts, which is thought to be a regulator of tissue regeneration. Figuring out the effects of the osteoimmune microenvironment on the osteogenesis of bone grafts can be a valuable strategy for their design and can further enhance the healing of bone defects. Our previous study demonstrated that the silicocarnotite (Ca(PO)SiO, CPS) bioceramic can significantly promote osteogenesis. The aim of this study is to investigate the immune reaction of CPS, the effects of the immune microenvironment on osteogenesis, and the related molecular mechanisms. Compared to hydroxyapatite (Ca(PO)(OH), HA), the results showed that CPS could downregulate the pro-inflammatory phenotype and upregulate the anti-inflammatory phenotype, showing the lower levels of TNF-α and increased expression of IL-10. We further found that CPS could regulate the expression of NPPA, EDN1, and MMP9 in RAW 264.7 by RNA sequencing, which may be related to its superiority in osteogenesis. The osteogenic differentiation of rat bone marrow mesenchymal stem cells (rBMSCs) was subsequently studied in a macrophage-conditioned medium pretreated with CPS, and the medium caused a significant promotion of the osteogenic differentiation of rBMSCs, demonstrating that CPS can generate a favorable immune microenvironment to promote rBMSCs differentiation. In terms of mechanism, CPS in the macrophage-conditioned medium promoted osteogenic differentiation through the MAPK pathway, including ERK1/2, JNK and P38. Our study demonstrated that osteogenic differentiation was influenced by the immune microenvironment generated the implant, and also presented an effective tool for studying the mechanisms of macrophage polarization as well as functions.
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http://dx.doi.org/10.1039/d2bm00125jDOI Listing
May 2022

WWC proteins mediate LATS1/2 activation by Hippo kinases and imply a tumor suppression strategy.

Mol Cell 2022 05 15;82(10):1850-1864.e7. Epub 2022 Apr 15.

Institute of Pediatrics, Children's Hospital of Fudan University, and Shanghai Key Laboratory of Medical Epigenetics, International Co-laboratory of Medical Epigenetics and Metabolism, Institutes of Biomedical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China. Electronic address:

YAP and TAZ (YAP/TAZ), two major effectors of the Hippo signaling pathway, are frequently activated in human cancers. The activity of YAP/TAZ is strictly repressed upon phosphorylation by LATS1/2 tumor suppressors. However, it is unclear how LATS1/2 are precisely regulated by upstream factors such as Hippo kinases MST1/2. Here, we show that WWC proteins (WWC1/2/3) directly interact with LATS1/2 and SAV1, and SAV1, in turn, brings in MST1/2 to phosphorylate and activate LATS1/2. Hence, WWC1/2/3 play an organizer role in a signaling module that mediates LATS1/2 activation by MST1/2. Moreover, we have defined a minimum protein interaction interface on WWC1/2/3 that is sufficient to activate LATS1/2 in a robust and specific manner. The corresponding minigene, dubbed as SuperHippo, can effectively suppress tumorigenesis in multiple tumor models. Our study has uncovered a molecular mechanism underlying LATS1/2 regulation and provides a strategy for treating diverse malignancies related to Hippo pathway dysregulation.
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http://dx.doi.org/10.1016/j.molcel.2022.03.027DOI Listing
May 2022

Development of a rapid neutralization assay for the detection of neutralizing antibodies against coxsackievirus B1.

Diagn Microbiol Infect Dis 2022 Jun 16;103(2):115676. Epub 2022 Mar 16.

State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Life Sciences, School of Public Health, Xiamen University, Xiamen, PR China.

Coxsackievirus B1 (CVB1) is a major pathogen that causes viral myocarditis and aseptic meningitis and is implicated as a cause of type 1 diabetes mellitus. The rapid detection of neutralizing antibodies can help in the prevention and diagnosis of viral infection. The traditional cytopathic effect (CPE)-based neutralization assay (Nt-CPE) is time-consuming and labor-intensive. In this study, an efficient neutralization test based on an enzyme-linked immunospot assay and a monoclonal antibody 2E6 against CVB1 (Nt-Elispot) was developed. In this optimal Nt-Elispot, a multiplicity of infection (MOI) of 1 per well was set as the infection dose, and an incubation time of 18 hours was selected as the checkpoint. Compared with Nt-CPE, Nt-Elispot significantly shortened the detection period and displayed a good correlation with it. This established CVB1 Nt-Elispot could be applied to efficiently screen neutralizing antibodies and evaluate the level of NAb against CVB1 in large cohorts.
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http://dx.doi.org/10.1016/j.diagmicrobio.2022.115676DOI Listing
June 2022

Hybrid 3D printed integrated microdevice for the determination of copper ions in human body fluids.

Anal Bioanal Chem 2022 Jun 9;414(14):4047-4057. Epub 2022 Apr 9.

School of Chemistry, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China.

On-site screening of copper ions in body fluid plays a critical role in monitoring human health, especially in heavy pollution areas. In this study, we have developed a hybrid 3D printed integrated microdevice for the determination of copper ions in human body fluids. A fixed and low volume of sample was detected by using the integrated microdevice without any preprocessing. The hybrid channel enables sample uniform mixing and quantitative dilution with buffer solution by inducing the "horseshoe vortex" phenomenon. The electrolytic microcell based on the flow detection system shows a more effective copper ion reaction ratio and, as a result, a better sensitivity. The simulation of the finite element method (FEM) determined the relevant optimum parameters of the hybrid channel and the microcell. The design, fabrication, and detection procedure of the integrated microdevice are here illustrated. The microdevice presented superior detection properties towards copper ions. The calibration curves covered two linear ranges varying from 20 to 100 ppb and 100 to 400 ppb, respectively. The limit of detection was estimated to be 15 ppb (S/N = 3). The relative standard deviation of the peak current measurements was 2.26%. The designed microdevice was further applied to detect copper ions in practical samples (calf serum sample and synthetic human urine sample) using a standard addition method, and the average recovery was found to be 95-104%. The performance of copper ion detection with the integrated microdevice was consistent with that of the inductively coupled plasma mass spectrometry (ICP-MS) in the same practical samples, demonstrating significant practicality in the test of body fluidics. The portable integrated microdevice is an excellent choice for on-site detection and has a promising prospect in the point-of-care testing (POCT) applications.
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http://dx.doi.org/10.1007/s00216-022-04049-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8993678PMC
June 2022

Dosimetric and radiobiological comparison of simultaneous integrated boost radiotherapy for early stage right side breast cancer between three techniques: IMRT, hybrid IMRT and hybrid VMAT.

Radiat Oncol 2022 Mar 28;17(1):60. Epub 2022 Mar 28.

National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital & Shenzhen Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Shenzhen, 518116, China.

Purpose: This study aimed at evaluating the clinical impact of full intensity-modulated radiotherapy (IMRT), hybrid IMRT (H-IMRT) and hybrid volumetric-modulated arc therapy (H-VMAT) for early-stage breast cancer with simultaneous integrated boost (SIB), in terms of plan quality and second cancer risk (SCR).

Methods: Three different plans were designed in full IMRT, hybrid IMRT, and hybrid VMAT for each of twenty patients with early-stage breast cancer. Target quality, organs at risk (OARs) sparing, and SCR were compared among the three plans for each case.

Results: In compared with H-IMRT, IMRT plans showed deterioration in terms of D of SIB, V of ipsilateral lung, and excess absolute risk (EAR) to contralateral lung (C-Lung) and esophagus. D and the homogeneity index (HI) of SIB, V5 of ipsilateral lung (I-Lung), the D of the esophagus, the EAR to C-Lung and the esophagus with hybrid VMAT dramatically increased by 0.63%, 10%, 17.99%, 149.27%, 230.41%, and 135.29%, respectively (p = 0.024; 0.025; 0.046; 0.011; 0.000; 0.014). D of the heart, the EAR to contralateral breast (C-Breast) and C-Lung by full IMRT was significantly decreased in comparison to the H-VMAT (4.67%, p = 0.033, 26.76%, p = 0.018; 48.05%, p = 0.036).

Conclusion: The results confirmed that H-IMRT could achieve better target quality and OARs sparing than IMRT and H-VMAT for SIB radiotherapy of early-stage right breast cancer. H-IMRT was the best treatment option, while H-VMAT performed the worst among the three plans in terms of SCR to peripheral OARs.
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http://dx.doi.org/10.1186/s13014-022-02009-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8962055PMC
March 2022
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