Publications by authors named "Rui Wang"

5,578 Publications

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Study on the hydrodynamic performance and treatment effect of a modified biological carrier in wastewater treatment.

Sci Total Environ 2022 Jun 25:156974. Epub 2022 Jun 25.

SKLOE, CISSE, School of Naval Architecture, Ocean & Civil Engineering, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address:

A modified biological carrier used in the moving bed biofilm reactor was developed from the perspective of the constituent material and the structure, then its hydrodynamic performance and treatment effect in wastewater treatment were investigated, and the movement of the modified biological carriers was simulated by Flow 3D software. The results show that the specific surface area of the modified biological carrier prepared by mixing PA and HDPE materials is about 5.1 % higher than that of the existing biological carrier; the folded cylindrical structure is adopted, which makes the directions of the aeration and water flow though the interior of the modified biological carrier more deflected, and keeps the flushing and pushing with the flow all the time; the presence of the modified biological carriers plays a good role in the gas distribution, and a large number of vortices with different sizes are generated in the reactor, which enhances the mass transfer in the reactor; the average removal rate of COD is up to 88 %, and the maximum removal rate of NH-N is up to 95 %, which indicates that the treat effect of the bioreactor is enhanced by the modified biological carriers. The modified biological carrier has the better hydrodynamic performance and treatment effect, which has the prospect of the popularization and application in the field of wastewater treatment.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156974DOI Listing
June 2022

Suo Quan Wan ameliorates bladder overactivity and regulates neurotransmission via regulating Myosin Va protein expression.

Phytomedicine 2022 Jun 15;104:154265. Epub 2022 Jun 15.

School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China. Electronic address:

Background: Ancient prescriptions of Suo Quan Wan (SQW) have therapeutic effects on diabetic bladder dysfunction. However, the underlying mechanism remains unclear. Here, we hypothesized that SQW ameliorates bladder overactivity and regulates neurotransmission via regulating Myosin Va protein expression.

Methods: After diabetic rats were induced by streptozotocin (65 mg/kg), the model of diabetic bladder dysfunction was established by detecting fasting blood glucose, urodynamic test, in vitro muscle strip experiments, and histological examination. One week after induction, SQW was given to observe the therapeutic effect. The expression levels of Myosin Va in control, Model, SQW L and SQW H groups were detected by RT-qPCR, RNAscope and immunofluorescence assay. The expression levels of ChAT, SP, nNOS and VIP proteins were observed by immunofluorescence assay. After knockdown and overexpression of Myosin Va, the expression changes of ChAT, SP, nNOS and VIP and the regulatory role of SQW were observed.

Results: STZ-induced DM rats had significantly higher serum glucose levels and lower body weight. Compared with the diabetic rats, SQW treatment significantly improved urination function with decreased residual volume (RV), bladder compliance (BC), non-voiding contractions (NVCs), and increased voided efficiency (VE). In addition, contractile responses of muscle strips to electrical-field stimulation (EFS), carbachol (CCh), KCl were significantly lower in the SQW H and SQW L groups than those in the model group. RT-qPCR found that the expression of Myosin Va in the bladder tissue or bladder neurons in model group was significantly increased compared with the control group, and SQW treatment significantly decreased the levels of Myosin Va. In DM rats, ChAT and SP expression were significantly increased, while nNOS and VIP expression were significantly decreased, and SQW improved this phenomenon. Interestingly, SQW ameliorated the abnormal expression of ChAT, SP, nNOS and VIP caused by myosin Va knockdown, and Myosin Va overexpression results are consistent with these.

Conclusions: SQW ameliorates overactive bladder and regulate neurotransmission via regulating Myosin Va mRNA and protein expression.
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http://dx.doi.org/10.1016/j.phymed.2022.154265DOI Listing
June 2022

RiceNCexp: A Resource for Rice Non-coding RNA Co-Expression Atlas Based on Massive RNA-seq and Small-RNA seq Data.

J Exp Bot 2022 Jun 28. Epub 2022 Jun 28.

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.

Non-coding RNAs (ncRNAs) play important roles in regulating expression of protein-coding genes. Although gene expression databases have emerged timely, however, the comprehensive expression database for ncRNAs is still lacking. Herein, we constructed a rice non-coding RNA co-expression atlas (RiceNCexp), based on 491 RNA-seq and 274 sRNA-seq datasets. RiceNCexp hosts four types of ncRNAs including lncRNA, PHAS gene, microRNA and phasiRNA. RiceNCexp provides comprehensive expression information for rice ncRNAs in 22 tissues/organs, an efficient tau-based mining tool for tissue-specific ncRNAs and the robust co-expression analysis among ncRNAs or between ncRNAs and protein-coding genes, based on 116 pairs of RNA-seq and small RNA-seq libraries from the same experiments. In summary, RiceNCexp is a user-friendly and comprehensive rice ncRNA co-expression atlas and can be freely accessed at h ttps://cbi.njau.edu.cn/RiceNCexp/.
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http://dx.doi.org/10.1093/jxb/erac285DOI Listing
June 2022

Integrated metabolomics and transcriptome analysis on flavonoid biosynthesis in flowers of safflower ( L.) during colour-transition.

PeerJ 2022 22;10:e13591. Epub 2022 Jun 22.

State Key Laboratory of Southwestern Chinese Medicine Resources, Chengdu, Sichuan, China.

Background: Safflower ( L.), well known for its flower, is widely used as a dye and traditional Chinese medicine. Flavonoids, especially flavonoid glycosides, are the main pigments and active components. However, their biosynthesis is largely unknown. Interestingly, the colour of flowers in safflower changed from yellow to red during flower development, while much of the gene and chemical bases during colour transition are unclear.

Methods: In this research, widely targeted metabolomics and transcriptomics were used to elucidate the changes in flavonoid biosynthesis from the gene and chemical points of view in flowers of safflower during colour transition. The screening of differential metabolites depended on fold change and variable importance in project (VIP) value. Differential expressed genes (DEGs) were screened by DESeq2 method. RT-PCR was used to analyse relative expressions of DEGs.

Results: A total of 212 flavonoid metabolites, including hydroxysafflor yellow A, carthamin and anthocyanins, were detected and showed a large difference. The candidate genes of glycosyltransferases and flavonoid hydroxylase that might participate in flavonoid glycoside biosynthesis were screened. Ten candidate genes were screened. Through integrated metabolomics and transcriptome analysis, a uridine diphosphate glucose glycosyltransferase gene, showed a significant correlation with flavonoid glycosides in safflower. In addition, expression analysis showed that was mainly expressed in the middle development of flowers and was significantly upregulated under MeJA treatment. Our results indicated that might play an important role in flavonoid glycoside biosynthesis during colour-transition in safflower.
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http://dx.doi.org/10.7717/peerj.13591DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9233481PMC
June 2022

Unveiling the Synthetic Potential of 1,3,5-Tri(10-phenothiazin-10-yl)benzene-Based Optoelectronic Material: A Metal-Free and Recyclable Photocatalyst for Sequential Functionalization of C(sp)-H Bonds.

ACS Appl Mater Interfaces 2022 Jun 27. Epub 2022 Jun 27.

Fujian Key Laboratory of Polymer Materials, Fujian Provincial Key Laboratory of Advanced Materials Oriented Chemical Engineering, College of Chemistry and Materials Science, Fujian Normal University, 8 Shangsan Lu, Fuzhou 350007, China.

1,3,5-Tri(10-phenothiazin-10-yl)benzene (3PTZ) is endowed with unique redox and photoresponsive characteristics and has been utilized as a p-type redox center for organic battery cathode material and a room-temperature phosphorescence (RTP) material, respectively. Conversely, its exploration in other research fields, particularly organic synthesis, remains unknown. Here, we demonstrate that 3PTZ-POP synthesized via cross-linking of 3PTZ is capable of harvesting visible-light photons and selectively converting solar energy to chemical energy. Specifically, 3PTZ-POP functions as a metal-free and recyclable photocatalyst to promote the sequential C(sp)-H functionalizations of -arylacrylamides with readily available trifluoromethylsulfonyl chloride as the radical precursor. An array of 3,3-disubstituted 2-oxindoles bearing a pharmaceutically important CF moiety are delivered in moderate to excellent yields under mild and sustainable conditions.
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http://dx.doi.org/10.1021/acsami.2c08766DOI Listing
June 2022

Nature of Five-Coordinated Al in γ-AlO Revealed by Ultra-High-Field Solid-State NMR.

ACS Cent Sci 2022 Jun 23;8(6):795-803. Epub 2022 May 23.

State Key Laboratory of Catalysis, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China.

Five-coordinated Als (Al(V)) on the surface of aluminas play important roles when they are used as catalysts or catalyst supports. However, the comprehensive characterization and understanding of the intrinsic structural properties of the Al(V) remain a challenge, due to the very small amount in commonly used aluminas. Herein, the surface structures of γ-AlO and Al(V)-rich AlO nanosheets (AlO-NS) have been investigated and compared in detail by multinuclear high-field solid-state NMR. Thanks to the high resolution and sensitivity of ultra-high-field (up to 35.2 T) NMR, the arrangements of surface Als were clearly demonstrated, which are substantially different from the bulk phase in γ-AlO due to the structure reconstruction. It reveals for the first time that most of the commonly observed Al(V)s tend to exist as aggregated states on the surface of γ-AlO, like those in amorphous AlO-NS liable to structure reconstruction. Our new insights into surface Al(V) species may help in understanding the structure-function relationship of alumina.
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http://dx.doi.org/10.1021/acscentsci.1c01497DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228550PMC
June 2022

Potential of circulating lncRNA CASC2 as a biomarker in reflecting the inflammatory cytokines, multi-organ dysfunction, disease severity, and mortality in sepsis patients.

J Clin Lab Anal 2022 Jun 26:e24569. Epub 2022 Jun 26.

Department of Pharmacology, Cangzhou Medical College, Cangzhou, China.

Background: Long noncoding RNA (lncRNA) cancer susceptibility candidate gene 2 (CASC2) inhibits inflammation and multi-organ dysfunction in various ways. The present study was intended to explore the potency of blood lncRNA CASC2 as a biomarker for sepsis management.

Methods: Totally, 184 sepsis patients and 30 healthy controls were enrolled. The reverse transcription-quantitative polymerase chain reaction was used to detect lncRNA CASC2 expression in peripheral blood mononuclear cell samples from the subjects. Mortality during 28 days was recorded in sepsis patients.

Results: LncRNA CASC2 was decreased in sepsis patients [median (interquartile range [IQR]): 0.473 (0.241-0.773)] by comparison to healthy controls [median (IQR): 1.019 (0.676-1.685)] (p < 0.001). In sepsis patients, lncRNA CASC2 was negatively correlated with Acute Physiology and Chronic Health Evaluation II (APACHE II) (p = 0.001), Sequential Organ Failure Assessment (SOFA) (p < 0.001), SOFA-respiratory system (p = 0.010), SOFA-coagulation (p = 0.020), SOFA-liver (p = 0.019), and SOFA-renal (p = 0.010) scores, but was not related to SOFA-nervous (p = 0.466) and SOFA-cardio vascular system (p = 0.059) scores. Additionally, lncRNA CASC2 was negatively related to tumor necrosis factor-α (p = 0.024), interleukin (IL)-1β (p = 0.013), and IL-17A (p = 0.002), but was not linked to IL-6 (p = 0.112) or IL-10 (p = 0.074). Furthermore, lncRNA CASC2 was lower in sepsis deaths [median (IQR): 0.286 (0.166-0.475)] than in survivors [median (IQR): 0.534 (0.296-0.811)] (p < 0.001). Simultaneously, Kaplan-Meier (KM) curve analysis also observed that lncRNA CASC2 was inversely related to accumulating mortality in sepsis patients (p = 0.003). While lncRNA CASC2 could independently predict lower mortality risk.

Conclusion: Circulating lncRNA CASC2 inadequacy indicates the release of inflammatory cytokines, severe multi-organ injuries, and increased mortality in sepsis patients.
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http://dx.doi.org/10.1002/jcla.24569DOI Listing
June 2022

A review of transgenerational and multigenerational toxicology in the in vivo model animal Caenorhabditis elegans.

J Appl Toxicol 2022 Jun 26. Epub 2022 Jun 26.

Medical School, Southeast University, Nanjing, China.

A large number of pollutants existing in the environment can last for a long time, and their potential toxic effects can transfer from parents to their offspring. Thus, it is necessary to investigate the toxicity of environmental pollutants across multigenerations and the underlying mechanisms in organisms. Due to its short life cycle and sensitivity to environmental exposures, Caenorhabditis elegans is an important animal model for toxicity assessment of environmental pollutants across multigenerations. In this review, we introduced the transgenerational and multigenerational toxicity caused by various environmental pollutants in C. elegans. Moreover, we discussed the underlying mechanisms for the observed transgenerational and multigenerational toxicity of environmental contaminants in C. elegans.
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http://dx.doi.org/10.1002/jat.4360DOI Listing
June 2022

MCPIP1 promotes cell proliferation, migration and angiogenesis of glioma via VEGFA-mediated ERK pathway.

Exp Cell Res 2022 Jun 22;418(1):113267. Epub 2022 Jun 22.

Department of Anatomy, School of Basic Medicine, Anhui Medical University, Hefei, Anhui, 230032, China. Electronic address:

Glioma is the most common primary malignant intracranial tumor in the population, and is often associated with abundant angiogenesis. However, how angiogenesis is regulated during glioma progression is still poorly understood. Data mining of cancer patient database shows that MCPIP1 is positively correlated with VEGFA expression and negatively with survival. In this study, we report that overexpressed MCPIP1 in glioma cells is a boost of angiogenesis. Mechanistically, MCPIP1 upregulates the expression of VEGFA in glioma, and promote the secretion of VEGFA to the surroundings, which could stimulate angiogenesis through ERK pathway. Blocking VEGFA expression and secretion inhibited MCPIP1-mediated angiogenesis and glioma progression in vitro and xenograft models. Collectively, these results identify a critical role for MCPIP1 in angiogenesis and glioma progression by regulating the VEGFA-mediated ERK pathway, suggesting that targeting MCPIP1 may be a potential glioma-selective therapeutic strategy.
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http://dx.doi.org/10.1016/j.yexcr.2022.113267DOI Listing
June 2022

Smartphone-integrated fluorescent quenching immunochromatographic test strips designed for the determination of sesame allergens in food samples.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Jun 17;280:121522. Epub 2022 Jun 17.

State Key Laboratory for Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China. Electronic address:

A highly sensitive smartphone-integrated fluorescence quenching immunochromatographic assay (FQICA) for the detection of sesame allergen was proposed. Sesame antibodies were adsorbed on the surface of the gold nanoparticles to form fluorescent acceptors (AuNPs-Ab). Ovalbumin (OVA) protein was labeled with quantum dots (QDs) to form signal probes (QDs-OVA), which were coated on the C-line of the assay strips. A mixture of QDs-OVA and sesame protein was coated on the T-line of the strip. For FQICA, the concentration of the analyte was positively correlated with the fluorescence signal. The developed FQICA had high sensitivity for the detection of sesame protein, and its visual LOD was 80 μg/L and the quantitative LOD was 40 μg/L. In addition, the method had high specificity, except for a small cross-reaction between sesame and walnut. The visual LODs in bread, ham, and biscuits were 640 μg/L. The quantitative LODs were 320 μg/L for biscuits and 640 μg/L for bread and ham. Comparing the developed FQICA with a commercial ELISA kit, the recoveries of sesame protein in both methods were between 80% and 120%.
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http://dx.doi.org/10.1016/j.saa.2022.121522DOI Listing
June 2022

Metal organic framework derived perovskite/spinel heterojunction as efficient bifunctional oxygen electrocatalyst for rechargeable and flexible Zn-air batteries.

J Colloid Interface Sci 2022 Jun 14;625:502-511. Epub 2022 Jun 14.

Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan, 430074, China; Engineering Research Center of Nano-Geomaterials of Ministry of Education, China University of Geosciences, 388 Lumo Road, Wuhan 430074, China.

Interface engineering strategy has been developed to design efficient catalysts for boosting electrocatalytic performance in past few decades. Herein, heterojunctions of PrCoO/CoO nanocages (PCO/CoO NCs) with atomic-level engineered interfaces and rich oxygen vacancies are proposed for Zn-air batteries. The synthesized product shows exceptional bifunctional activity and robust stability towards oxygen reduction reaction (ORR) and oxygen evolution reaction (OER). The enhanced catalytic capacity is primary attributed to the synergistic effect of PCO/CoO, evidenced by the experimental results and theoretical calculations. More importantly, the PCO/CoO NCs assembled liquid Zn-air battery exhibits a power density of 182 mW cm and a long-term operation of 185 h. When assembled into solid-state cable type battery, this newly designed catalyst also reaches a stable open circuit voltage (1.359 V) and a peak power density of 85 mW cm. Our findings provide essential guidelines of engineering heterostructured electrocatalysts for future wearable electronic devices.
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http://dx.doi.org/10.1016/j.jcis.2022.06.048DOI Listing
June 2022

Optimization of Naringenin Nanoparticles to Improve the Antitussive Effects on Post-Infectious Cough.

Molecules 2022 Jun 10;27(12). Epub 2022 Jun 10.

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 151, Malianwa North Road, Haidian District, Beijing 100193, China.

Naringenin (NRG) is a natural compound with several biological activities; however, its bioavailability is limited owing to poor aqueous solubility. In this study, NRG nanoparticles (NPs) were prepared using the wet media milling method. To obtain NRG NPs with a small particle size and high drug-loading content, the preparation conditions, including stirring time, temperature, stirring speed, and milling media amount, were optimized. The NRG (30 mg) and D-α-tocopherol polyethylene glycol succinate (10 mg) were wet-milled in deionized water (2 mL) with 10 g of zirconia beads via stirring at 50 °C for 2 h at a stirring speed of 300 rpm. As a result, the NRG NPs, with sheet-like morphology and a diameter of approximately 182.2 nm, were successfully prepared. The NRG NPs were stable in the gastrointestinal system and were released effectively after entering the blood circulation. In vivo experiments indicated that the NRG NPs have good antitussive effects. The cough inhibition rate after the administration of the NRG NPs was 66.7%, cough frequency was three times lower, and the potential period was 1.8 times longer than that in the blank model group. In addition, the enzyme biomarkers and histological analysis results revealed that the NRG NPs can effectively regulate the inflammatory and oxidative stress response. In conclusion, the NRG NPs exhibited good oral bioavailability and promoted antitussive and anti-inflammatory effects.
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http://dx.doi.org/10.3390/molecules27123736DOI Listing
June 2022

Co-Utilization of Sewage Sludge and Rice Husk in Ceramsite Preparation with Selective Adsorption Capacity to Pb.

Materials (Basel) 2022 Jun 17;15(12). Epub 2022 Jun 17.

State Key Laboratory of Water Resources and Hydropower Engineering Science, Wuhan University, Wuhan 430072, China.

Realizing the green recycling of sludge is an important link to effectively solve the problem of sludge disposal. In this paper, sewage sludge (SS) and rice husk (RH) were utilized as raw materials in preparing novel ceramsite (SRC) for the treatment of lead-containing wastewater, and its adsorption mechanism was explored. The results showed that the optimal preparation conditions were 40% RH + 60% SS mixture, a sintering temperature of 1190 °C, and a sintering time of 20 min. The basic properties of SRC met Chinese artificial ceramsite filter material standards for water treatment (CJ/T 299-2008). Under optimum adsorption conditions (pH = 6, 1 g/L SRC dosage, 20 mg/L Pb(NO) concentration, 18 h), the removal rate of Pb reached 94.7%, and the equilibrium adsorption capacity was 18.94 mg/g. The adsorption process was more consistent with the pseudo-second-order kinetic model and the Langmuir isotherm model, indicating that the adsorption process was dominated by chemisorption. Thermodynamic parameters (ΔH > 0, ΔG < 0, ΔS > 0) indicated that the adsorption reaction was spontaneous and endothermic. The possible adsorption mechanisms are as follows: (1) SRC is rich in layered mesoporous structure, which provides sufficient reaction sites for Pb adsorption; (2) the sintered lawsonite and muscovite can strongly attract Pb and then form a new phase (Pb[SiO](OH)); (3) Pb can bond with the Si-O- bond in aluminosilicates, and the introduction of Pb elevates the degree of polymerization of aluminosilicates in turn, indicating that the adsorption process is stable.
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http://dx.doi.org/10.3390/ma15124310DOI Listing
June 2022

Understanding Impacts of SNAP Fruit and Vegetable Incentive Program at Farmers' Markets: Findings from a 13 State RCT.

Int J Environ Res Public Health 2022 Jun 17;19(12). Epub 2022 Jun 17.

Musconetcong Watershed Association, Asbury, NJ 08802, USA.

Disparities in healthy food access and consumption are a major public health concern. This study reports the findings from a two-year randomized control trial conducted at 77 farmers' markets (FMs) in 13 states and the District of Columbia that sought to understand the impact of fruit and vegetable (FV) incentive vouchers, randomly issued at varied incentive levels to Supplemental Nutrition Assistance Program (SNAP) recipients, for use at FMs. Measures included FV and overall household food purchasing; FV consumption; food insecurity; health status; market expenditure; and demographics. A repeated-measures mixed-effects analysis and the Complier Average Causal Effect (CACE) were used to examine outcomes. Despite 82% reporting food insecurity in the prior year, the findings showed that financial incentives at FMs had statistically significant, positive effects on FV consumption; market expenditures increased with added incentives. SNAP recipients receiving an incentive of USD 0.40 for every USD 1.00 in SNAP spent an average of USD 19.03 per transaction, while those receiving USD 2 for every USD 1 (2:1) spent an average of USD 36.28 per transaction. The data showed that the incentive program at the highest level (2:1) maximally increased SNAP FM expenditure and FV consumption, increasing the latter by 0.31 daily cups among those who used their incentive (CACE model).
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http://dx.doi.org/10.3390/ijerph19127443DOI Listing
June 2022

Asymmetric Ferroelectric-Gated Two-Dimensional Transistor Integrating Self-Rectifying Photoelectric Memory and Artificial Synapse.

ACS Nano 2022 Jun 22. Epub 2022 Jun 22.

School of Physics, Henan Key Laboratory of Photovoltaic Materials, Henan Normal University, Xinxiang 453007, China.

Ferroelectric field-effect transistors (Fe-FET) are promising candidates for future information devices. However, they suffer from low endurance and short retention time, which retards the application of processing memory in the same physical processes. Here, inspired by the ferroelectric proximity effects, we design a reconfigurable two-dimensional (2D) MoS transistor featuring with asymmetric ferroelectric gate, exhibiting high memory and logic ability with a program/erase ratio of over 10 and a self-rectifying ratio of 10. Interestingly, the robust electric and optic cycling are obtained with a large switching ratio of 10 and nine distinct resistance states upon optical excitation with excellent nonvolatile characteristics. Meanwhile, the operation of memory mimics the synapse behavior in response to light spikes with different intensity and number. This design realizes an integration of robust processing memory in one single device, which demonstrates a considerable potential of an asymmetric ferroelectric gate in the development of Fe-FETs for logic processing and nonvolatile memory applications.
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http://dx.doi.org/10.1021/acsnano.2c04271DOI Listing
June 2022

E2F7 promotes mTOR inhibitor resistance in hepatocellular carcinoma after liver transplantation.

Am J Transplant 2022 Jun 21. Epub 2022 Jun 21.

Department of Hepatobiliary and Pancreatic Surgery, The Center for Integrated Oncology and Precision Medicine, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, China.

The mammalian target of rapamycin (mTOR) pathway is frequently de-regulated and has critical roles in cancer progression. mTOR inhibitors has been widely used in several kinds of cancers and is strongly recommended in patients with hepatocellular carcinoma (HCC) after liver transplantation (LT). However, the poor response to mTOR inhibitors due to resistance remains a challenge. Hypoxia-associated resistance limits the therapeutic efficacy of targeted drugs. The present study established models of HCC clinical samples and cell lines resistance to mTOR inhibitor sirolimus and screened out E2F7 as a candidate gene induced by hypoxia and promoting sirolimus resistance. E2F7 suppressed mTOR complex 1 via directly binding to the promotor of TSC1 gene, and stabilizes hypoxia-inducible factor-1α (HIF-1α) activating its downstream genes, which are responsible for E2F7 dependent mTOR inhibitor resistance. Clinically, low E2F7 expression could be an effective biomarker for recommending HCC patients for anti-mTOR-based therapies after LT. Targeting E2F7 synergistically inhibited HCC growth with sirolimus in vivo. E2F7 is a promising target to reverse mTOR inhibition resistance. Collectively, our study points to a role for E2F7 in promoting mTOR inhibitor resistance in HCC and emphasize its potential clinical significance in HCC patients after LT.
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http://dx.doi.org/10.1111/ajt.17124DOI Listing
June 2022

A novel approach for treating acid mine drainage by forming schwertmannite driven by a combination of biooxidation and electroreduction before lime neutralization.

Water Res 2022 Jun 14;221:118748. Epub 2022 Jun 14.

Department of Environmental Engineering, School of Information and Safety Engineering, Zhongnan University of Economics and Law, Wuhan 430073, China. Electronic address:

Acid mine drainage (AMD) contains abundant iron, sulfates, and various metal ions, and it causes environmental pollution. The traditional AMD lime neutralization forms a layer of iron hydroxide and gypsum on the surface of the lime particles, preventing continuous reaction and leading to excessive lime addition and neutralized sludge production. In this study, an approach for treating AMD using a cyclic process of biooxidation and electroreduction before lime neutralization was proposed, in which the Fe in AMD was oxidized to Fe and induced to form schwertmannite through Acidithiobacillus ferrooxidans. The remaining Fe was reduced to Fe using an electric field. After three biooxidation and two electroreduction cycles, 98.2% of Fe and 62.4% of SO in AMD precipitated as schwertmannite (FeO(OH)(SO)). The yield of schwertmannite reached 33.98 g/L, with a high specific surface area of 112.59 m/g. The lime dosage and sludge yield of the treated AMD in the subsequent neutralization stage (pH = 7.00) decreased by 85.0% and 74.5%, respectively, than those of raw AMD. The pilot test results showed that the integrated treatment of biooxidation-electroreduction cyclic mineralization and lime neutralization has practical applications.
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http://dx.doi.org/10.1016/j.watres.2022.118748DOI Listing
June 2022

Using Advanced Analytic Techniques to Optimize Cyber-Physical Defensive Plans in Sports Infrastructures and Facilities.

Authors:
Rui Wang

Comput Intell Neurosci 2022 11;2022:2061769. Epub 2022 Jun 11.

Zhengzhou Preschool Education College, Zhengzhou, Henan 450000, China.

The technical projects for securing a network of infrastructures and processes are designed, financed, carried out, maintained, and operated within a general infrastructure system, which can gather activities and funds of the private or public sector. The importance of such projects for modern society is enormous, and there is a positive correlation between the size of infrastructure projects and the strength of the national economy. At the same time, it falls within the critical infrastructure sector most of the time. This work, taking into account the massive importance of investments made in the field of sports and the corresponding significance of the design and implementation of robust cybersecurity systems, presents an innovative optimization system for the design, performance, and adaptation of safeguards of technical projects, which require a high level of security standards. A realistic optimization system of low computational complexity is proposed and tested, dividing the problem into a series of subproblems of one-step optimization, which can be solved with great ease and without requirements on computational resources. The great innovation of the proposed system is that the separation is done so that the solution results from the optimal individual solutions of the subproblems without affecting the final result.
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http://dx.doi.org/10.1155/2022/2061769DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9206585PMC
June 2022

Low-noise frequency-agile photonic integrated lasers for coherent ranging.

Nat Commun 2022 Jun 20;13(1):3522. Epub 2022 Jun 20.

Institute of Physics, Swiss Federal Institute of Technology Lausanne (EPFL), CH-1015, Lausanne, Switzerland.

Frequency modulated continuous wave laser ranging (FMCW LiDAR) enables distance mapping with simultaneous position and velocity information, is immune to stray light, can achieve long range, operate in the eye-safe region of 1550 nm and achieve high sensitivity. Despite its advantages, it is compounded by the simultaneous requirement of both narrow linewidth low noise lasers that can be precisely chirped. While integrated silicon-based lasers, compatible with wafer scale manufacturing in large volumes at low cost, have experienced major advances and are now employed on a commercial scale in data centers, and impressive progress has led to integrated lasers with (ultra) narrow sub-100 Hz-level intrinsic linewidth based on optical feedback from photonic circuits, these lasers presently lack fast nonthermal tuning, i.e. frequency agility as required for coherent ranging. Here, we demonstrate a hybrid photonic integrated laser that exhibits very narrow intrinsic linewidth of 25 Hz while offering linear, hysteresis-free, and mode-hop-free-tuning beyond 1 GHz with up to megahertz actuation bandwidth constituting 1.6 × 10 Hz/s tuning speed. Our approach uses foundry-based technologies - ultralow-loss (1 dB/m) SiN photonic microresonators, combined with aluminium nitride (AlN) or lead zirconium titanate (PZT) microelectromechanical systems (MEMS) based stress-optic actuation. Electrically driven low-phase-noise lasing is attained by self-injection locking of an Indium Phosphide (InP) laser chip and only limited by fundamental thermo-refractive noise at mid-range offsets. By utilizing difference-drive and apodization of the photonic chip to suppress mechanical vibrations of the chip, a flat actuation response up to 10 MHz is achieved. We leverage this capability to demonstrate a compact coherent LiDAR engine that can generate up to 800 kHz FMCW triangular optical chirp signals, requiring neither any active linearization nor predistortion compensation, and perform a 10 m optical ranging experiment, with a resolution of 12.5 cm. Our results constitute a photonic integrated laser system for scenarios where high compactness, fast frequency actuation, and high spectral purity are required.
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http://dx.doi.org/10.1038/s41467-022-30911-6DOI Listing
June 2022

Chemokine (C-C motif) receptor 2 is associated with the pathological grade and inflammatory response in IgAN children.

BMC Nephrol 2022 Jun 20;23(1):215. Epub 2022 Jun 20.

Department of Pediatrics, First Affiliated Hospital of Anhui Medical University, 19Th Floor of Medicine and Medical Tech Building, 218 Jixi Road, Hefei, 230022, Anhui, China.

Background: Chemokine (C-C motif) receptor 2 (CCR2) is involved in important physiological and pathological processes, such as inflammation and autoimmune diseases. Abnormal immune and inflammatory responses play a critical role in the development and progression of IgA nephritis (IgAN). However, the role of CCR2 in IgAN is unknown.

Methods: Fifteen IgAN children who were diagnosed by kidney biopsy provided kidney biopsy tissue, blood and urine samples, and age-matched healthy control subjects (blood donators n = 12; tissue donators n = 8) were included. Immunohistochemical analysis was used to detect the expression of CCR2, MCP-1, IL-6, IL-17, and TNF-α in the kidney tissues. Relative optical density (OD) was calculated by Image J software, and the correlation between CCR2 expression and pathological grade in IgAN children was analyzed.

Results: The expression of CCR2 significantly increased in mesangial cells of children with IgAN compared to that in control group (P < 0.001), especially in IgAN patients with Lee's grade III to IV (P < 0.001). Interestingly, CCR2 expression was positively correlated with Lee's grade (r = 0.9152, P = 0.0001) in IgAN children. The expression levels of inflammatory factors were markedly increased in IgAN children, and importantly CCR2 expression was positively correlated with it's expression level.

Conclusions: The results suggest that CCR2 signaling might be involved in pathological process and inflammatory responses of children IgAN, and could potentially be an intervention target in children IgAN.
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http://dx.doi.org/10.1186/s12882-022-02839-yDOI Listing
June 2022

Phospholipase A inhibitor varespladib prevents wasp sting-induced nephrotoxicity in rats.

Toxicon 2022 Jun 17;215:69-76. Epub 2022 Jun 17.

Department of Nephrology, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyang, 441000, China. Electronic address:

This study aimed to clarify whether varespladib, a phospholipase A (PLA) inhibitor, can be used as a therapeutic agent for wasp sting-induced acute kidney injury (AKI). Rats were divided into control, AKI, and AKI + varespladib groups. The AKI model was established by subcutaneously injecting wasp venom at five different sites in rats. Varespladib treatment showed a significant inhibitory effect on wasp venom PLAin vitro and in vivo. Moreover, we observed that varespladib decreased the levels of rhabdomyolysis and hemolysis markers compared with that in the AKI group. Histopathological changes in the kidney decreased significantly, and rat serum creatinine levels were reduced after varespladib administration. The significantly regulated genes in the kidney of the AKI group were mostly involved in inflammatory response pathway, and the administration of varespladib remarkably attenuated the expression of these genes. Therefore, varespladib inhibited wasp sting-induced functional and pathological damage to the kidneys. We propose that the PLA inhibitor varespladib protects the kidney tissue in a wasp sting-induced AKI model by inhibiting PLA activity.
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http://dx.doi.org/10.1016/j.toxicon.2022.06.007DOI Listing
June 2022

Anatomic identification of laparoscopic uterosacral ligament suspension: A step-by-step procedure.

Int Urogynecol J 2022 Jun 20. Epub 2022 Jun 20.

Department of Gynecology, Women's Hospital, School of Medicine, Zhejiang University, 1st Xueshi Road, Hangzhou, Zhejiang, People's Republic of China, 310006.

Introduction And Hyposthesis: The uterosacral ligament (USL) is the main structure of physiological apical support, and USL suspension is one of the most commonly used methods for native tissue apical suspension. Structures surrounding the USL are complex, and the USL itself is difficult to identify, especially the sacral portion of the USL. Laparoscopy improves visualization, but exposure of the USL is still unsatisfactory.

Methods: In this study, we report a simple method for exposing and suturing the USL laparoscopically, with step-by-step instructions, well-presented figures and videos. The key techniques are shown as follows: keeping tension on the USL with a uterine manipulator, dissecting the space medial to the USL, exposing the portion of the USL near the sacrum through the natural space, and then suturing it medially and connecting it directly to the posterior cervix.

Results: 95 consecutive patients have undergone this modified USLS and none had serious perioperative complication.

Conclusion: In this way, the USL anatomy is exposed well, which may make placement of sutures in USL suspension safe and effective.
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http://dx.doi.org/10.1007/s00192-022-05257-8DOI Listing
June 2022

Roles of Exosome Genomic DNA in Colorectal Cancer.

Front Pharmacol 2022 1;13:923232. Epub 2022 Jun 1.

Department of Stem Cells and Regenerative Medicine, Key Laboratory of Cell Biology, National Health Commission of China, and Key Laboratory of Medical Cell Biology, Ministry of Education of China, China Medical University, Shenyang, China.

Exosomes are extracellular vesicles that mediate cell-to-cell communication. Bioactive substances such as DNA, RNA, lipids, and proteins are present in it, and they play an essential role in the pathogenesis of colorectal cancer (CRC). The role of RNA and protein in exosomes has been extensively studied. Exosome DNA has recently attracted the attention of a great deal of scientists. According to studies, exosome DNA mainly contains genomic DNA (gDNA) and mitochondrial DNA (mtDNA), of which exosome gDNA is widely used in liquid biopsy of CRC. It includes a variety of clinically relevant tumor-specific mutation genes. In addition to liquid biopsy, researchers find that exosome gDNA regulates immune and metabolic functions in CRC, making it an important research object. However, the primary research on exosome gDNA is still limited. Here, we describe the occurrence and composition of exosomes. Summarize the essential characteristics and mode of action of exosome gDNA. Remarkably, this paper constitutes a comprehensive summary on the role of exosome gDNA on CRC with the intent of providing a theoretical basis and reference for early diagnosis and clinical treatment of cancer.
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http://dx.doi.org/10.3389/fphar.2022.923232DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9198365PMC
June 2022

[Vitamin A nutrition status of Chinese urban adults aged 18 to 60 years old in 2015].

Wei Sheng Yan Jiu 2022 May;51(3):381-385

NHC Key Laboratory of Trace Element Nutrition, National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China.

Objective: To investigate the vitamin A nutritional status and its related influencing factors of Chinese 18-60 urban adults by analyzing serum retinol level in 2015.

Methods: Serum samples of 42 people, half male and half female, were randomly selected in each point, from the 302 monitoring sites of Chinese adult chronic diseases and nutrition surveillance in 2015. The serum retinol levels were determined by high pressure liquid chromatography(HPLC).

Results: A total of 2571 serum samples were detected. The serum retinol level of Chinese urban adults of 18-60 years old was 1.98(1.56-2.53)μmol/L, the deficiency rate was 0.25%, and the marginal deficiency rate was 4.45%. The serum retinol level of male was 2.19(1.75-2.78)μmol/L, the deficiency rate was 0.05%, and the marginal deficiency rate was 2.48%; The serum retinol level of female was 1.80(1.42-2.25)μmol/L, the deficiency rate was 0.46%, and the marginal deficiency rate was 6.51%. The significant differences in serum retinol was observed deficiency rate and marginal deficiency rate between males and females(P<0.01), the nutritional status of males was better than the females. The deficiency rate and marginal deficiency rate of 18-29 years old were the highest, which were 0.34% and 5.81% respectively. The deficiency rate and marginal deficiency rate of over 50 years old were the lowest, which were 0.15% and 2.60% respectively. The vitamin A deficiency rate and marginal deficiency rate of people with body mass index(BMI)<18.5 were 0.95% and 7.24%, 0.33% and 5.85% for BMI=18.5-23.9, 0.13% and 3.27% for BMI=24.0-27.9, respectively. The differences of vitamin A deficiency rate and marginal deficiency rate among different BMI were significant.

Conclusion: The vitamin A nutritional status of urban adults in China is good, the vitamin A deficiency rate is very low, and the incidence of vitamin A deficiency is also low. It is basically not a public health problem according the WHO standard.
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http://dx.doi.org/10.19813/j.cnki.weishengyanjiu.2022.03.006DOI Listing
May 2022

Identification of Upper Gastrointestinal Diseases during Screening Gastroscopy through Deep Convolutional Neural Network Algorithm.

Gastrointest Endosc 2022 Jun 16. Epub 2022 Jun 16.

Department of Clinical Research Center, Dazhou Central Hospital, Dazhou, Sichuan, China; Center of Intelligent Medicine, Computer Science, Sichuan University, Chengdu, Sichuan, China. Electronic address:

Background And Aims: The clinical application of gastrointestinal endoscopy for the diagnosis of multiple diseases using artificial intelligence (AI) has been limited by its high false positive rates. There is an unmet need to develop a Gastrointestinal Endoscopy AI-assisted Diagnosis System (GEADS) to improve diagnostic accuracy and clinical utility.

Methods: In this retrospective, multicenter study, a convolutional neural network was trained to assess upper gastrointestinal diseases based on 26,228 endoscopic images from Dazhou Central Hospital that were randomly assigned (3:1:1) to the training dataset, validation dataset, and test dataset, respectively. To validate the model, 6 external independent datasets comprising 51,372 images of upper gastrointestinal diseases were collected. Besides, one prospective dataset comprising 27,975 images were collected. The performance of GEADS was compared with endoscopists with two professional degrees of expertise: expert, novice. 8 endoscopists in the expert group with more than 5 years of experience, 3 endoscopists in the novice group with 1 to 5 years of experience.

Results: The GEADS model achieved an accuracy of 0.918 (95% confidence interval [CI]: 0.914-0.922), with an F1 score of 0.884 (95% CI: 0.879-0.889), recall of 0.873 (95% CI: 0.868-0.878) and a precision of 0.890 (95% CI: 0.885-0.895) in the internal validation dataset. In the external validation datasets and one prospective validation dataset, the diagnostic accuracy of GEADS ranged from 0.841 (95% CI: 0.834-0.848) to 0.949 (95% CI: 0.935-0.963). With the help of GEADS, the diagnosing accuracies of novice and expert endoscopists were significantly improved (P < 0.001).

Conclusions: The AI system can assist endoscopists in improving the accuracy of diagnosing upper gastrointestinal diseases.
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http://dx.doi.org/10.1016/j.gie.2022.06.011DOI Listing
June 2022

Autochthonous sources and drought conditions drive anomalous oxygen-consuming pollution increase in a sluice-controlled reservoir in eastern China.

Sci Total Environ 2022 Jun 15;841:156739. Epub 2022 Jun 15.

State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China. Electronic address:

Freshwater reservoirs are an important type of inland waterbody. However, they can suffer from oxygen-consuming pollution, which can seriously threaten drinking water safety and negatively impact the health of aquatic ecosystems. Oxygen-consuming pollutants originate from both allochthonous and autochthonous sources, and have temporally and spatially heterogeneous drivers. Datanggang Reservoir, China, is located in a small agricultural watershed; it is controlled by multiple sluice gates. Anomalously high oxygen consumption indicators were observed in this reservoir in March 2021. Here, it was hypothesized that autochthonous sources were the primary drivers of oxygen-consuming pollution in the reservoir under drought conditions. Datasets of water quality, precipitation, primary productivity, and sediment were used to analyze water quality trends in the reservoir and inflow rivers, demonstrating the effects of allochthonous inputs and autochthonous pollution. No correlation was found between reservoir oxygen consumption indicators and allochthonous inputs; reservoir oxygen consumption indicators and chlorophyll-a concentration were significantly positively correlated (p < 0.05). Substantially lower precipitation and higher water temperature and pH (compared to historical levels) were also observed before the pollution event. Therefore, during this period the hydrological conditions, water temperature, pH, and other variables caused by short-term drought conditions may have facilitated phytoplankton growth in the reservoir. This contributed to a large increase in autochthonous oxygen-consuming pollutants, as reflected by the abnormally high indicators. Sediments contaminated with organic matter may also have been an important contributor. As the effects of environmental management and pollution control continue to emerge, exogenous pollutants imported from the land to reservoirs are currently effectively controlled. However, endogenous pollutants driven by a variety of factors, such as meteorology and hydrology, will likely become the main drivers of short-term changes in oxygen-consuming pollution in freshwater reservoirs in the foreseeable future.
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http://dx.doi.org/10.1016/j.scitotenv.2022.156739DOI Listing
June 2022

BA.2.12.1, BA.4 and BA.5 escape antibodies elicited by Omicron infection.

Nature 2022 Jun 17. Epub 2022 Jun 17.

Biomedical Pioneering Innovation Center (BIOPIC), Peking University, Beijing, P.R. China.

SARS-CoV-2 Omicron sublineages BA.2.12.1, BA.4 and BA.5 exhibit higher transmissibility over BA.2. The new variants' receptor binding and immune evasion capability require immediate investigation. Here, coupled with Spike structural comparisons, we show that BA.2.12.1 and BA.4/BA.5 exhibit comparable ACE2-binding affinities to BA.2. Importantly, BA.2.12.1 and BA.4/BA.5 display stronger neutralization evasion than BA.2 against the plasma from 3-dose vaccination and, most strikingly, from post-vaccination BA.1 infections. To delineate the underlying antibody evasion mechanism, we determined the escaping mutation profiles, epitope distribution and Omicron neutralization efficacy of 1640 RBD-directed neutralizing antibodies (NAbs), including 614 isolated from BA.1 convalescents. Interestingly, post-vaccination BA.1 infection mainly recalls wildtype-induced humoral memory. The resulting elicited antibodies could neutralize both wildtype and BA.1 and are enriched on non-ACE2-competing epitopes. However, most of these cross-reactive NAbs are heavily escaped by L452Q, L452R and F486V. BA.1 infection can also induce new clones of BA.1-specific antibodies that potently neutralize BA.1; nevertheless, these NAbs are largely escaped by BA.2/BA.4/BA.5 due to D405N and F486V, and react weakly to pre-Omicron variants, exhibiting poor neutralization breadths. As for therapeutic NAbs, Bebtelovimab and Cilgavimab can effectively neutralize BA.2.12.1 and BA.4/BA.5, while the S371F, D405N and R408S mutations would undermine most broad sarbecovirus NAbs. Together, our results indicate that Omicron may evolve mutations to evade the humoral immunity elicited by BA.1 infection, suggesting that BA.1-derived vaccine boosters may not achieve broad-spectrum protection against new Omicron variants.
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http://dx.doi.org/10.1038/s41586-022-04980-yDOI Listing
June 2022

Dietary Pomace Supplementation Enhances the Expression of ZO-1 and Occludin and Promotes Intestinal Development in Pigs.

Front Vet Sci 2022 27;9:904667. Epub 2022 May 27.

State Key Laboratory for Zoonotic Diseases, Key Laboratory of Zoonosis Research, Ministry of Education, College of Animal Sciences, Jilin University, Changchun, China.

A fruit juice production byproduct, pomace (AMP) is rich in natural polyphenol antioxidant components. The objectives of this study were to study the effects of dietary AMP supplementation on the feeding outcome and intestinal barrier function of pigs. In total, 27 growing pigs (Duroc × Landrace × Yorkshire, ~60 days, average weight of 27.77 ± 2.87 kg, males and females included at random) were randomly allotted to 3 treatment groups, with 3 repetitions per group and 3 pigs per repetition. At the experiment completion, 2 pigs (close to the average body weight of all experimental pigs) per replicate were slaughtered. The control group (CON group) was fed a basic diet, and the experimental groups were fed 4% (4% AMP group) and 8% (8% AMP group) AMP in the basic diet. These pigs were prefed for 3 days, and the formal experiments were performed for 7 weeks. The results showed that compared with the CON diet, the 4% AMP supplementation significantly increased the average daily gain of pigs ( < 0.05). Regarding intestinal development, 4% AMP significantly increased the jejunal villus height/crypt depth ratio ( < 0.05), and different AMP levels had no significant effect on the pig cecum morphology. Different AMP levels significantly decreased the relative abundance of Proteobacteria ( < 0.05). Regarding other microbial genera, 4% AMP supplementation significantly increased the levels of and other beneficial microorganisms ( < 0.05). Different AMP levels significantly decreased the relative abundances of the opportunistic pathogens and ( < 0.05) and increased the contents of acetic acid and butyric acid in the pig cecal contents ( < 0.05). Compared with the CON treatment, 4% AMP supplementation significantly downregulated the jejunal gene expression of porcine proinflammatory factors (IL-1β, IL-6, IL-8 and TNF-α) and significantly upregulated the jejunal gene expression of ZO-1, Occludin and Claudin-1 ( < 0.05). In conclusion, 4% AMP supplementation in feed is beneficial to overall pig health and growth.
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http://dx.doi.org/10.3389/fvets.2022.904667DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9196908PMC
May 2022

A photonic integrated circuit-based erbium-doped amplifier.

Science 2022 06 16;376(6599):1309-1313. Epub 2022 Jun 16.

Institute of Physics, Swiss Federal Institute of Technology Lausanne (EPFL), CH-1015 Lausanne, Switzerland.

Erbium-doped fiber amplifiers revolutionized long-haul optical communications and laser technology. Erbium ions could provide a basis for efficient optical amplification in photonic integrated circuits but their use remains impractical as a result of insufficient output power. We demonstrate a photonic integrated circuit-based erbium amplifier reaching 145 milliwatts of output power and more than 30 decibels of small-signal gain-on par with commercial fiber amplifiers and surpassing state-of-the-art III-V heterogeneously integrated semiconductor amplifiers. We apply ion implantation to ultralow-loss silicon nitride (SiN) photonic integrated circuits, which are able to increase the soliton microcomb output power by 100 times, achieving power requirements for low-noise photonic microwave generation and wavelength-division multiplexing optical communications. Endowing SiN photonic integrated circuits with gain enables the miniaturization of various fiber-based devices such as high-pulse-energy femtosecond mode-locked lasers.
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http://dx.doi.org/10.1126/science.abo2631DOI Listing
June 2022

Development of Biocompatible Ene-Ligation Enabled by Prenyl-Based β-Caryophyllene with Triazoline/Selectfluor under Physiological Conditions.

J Org Chem 2022 Jun 16. Epub 2022 Jun 16.

The Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.

Here, we first report a rapid and highly selective biocompatible ligation that proceeds via a strain-promoted prenyl-involved [2, 3]-Ene rearrangement process. We demonstrate the usefulness of naturally occurring strain-promoted β-caryophyllene with triazoline (PTAD)/Selectfluor in the study of tagging molecule-of-interest. Experiments in peptide (Histone H3 (1-21) and Myhc (614-629)) and protein (BSA, βLG, and HSP40) models exemplified the utility of the Ene-ligation for in vivo imaging and tracking.
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http://dx.doi.org/10.1021/acs.joc.2c00841DOI Listing
June 2022
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