Publications by authors named "Rui Matos"

20 Publications

  • Page 1 of 1

Glomus Tumor: "A-Not-So-Rare" Cause of Chronic Shoulder Pain-A Case Report and Literature Review.

Case Rep Orthop 2021 27;2021:6666092. Epub 2021 May 27.

Centro Hospitalar Universitário São João, Portugal.

Case: A 61-year-old male presented with chronic shoulder pain resistant to conservative treatment. Imaging identified a nodular lesion in the deltoid muscle, which histology after resection identified as a glomus tumor. After surgery, the patient became asymptomatic and at 4-year follow-up has not shown signs of recurrence.

Conclusion: Glomus tumors around the shoulder should be considered when investigating chronic shoulder pain, as they are more common than thought. Despite being elusive, when diagnosed, excellent outcomes may be expected, with surgery resection being curative.
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http://dx.doi.org/10.1155/2021/6666092DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175186PMC
May 2021

Higher Physical Activity Levels May Help Buffer the Negative Psychological Consequences of Coronavirus Disease 2019 Pandemic.

Front Psychol 2021 22;12:672811. Epub 2021 Apr 22.

CIEQV - Life Quality Research Centre, Polytechnic of Leiria, Leiria, Portugal.

This study explored the associations between physical activity (PA) anxiety levels, and the perception of satisfaction of basic psychological needs (BPN), during Coronavirus Disease 2019 (COVID-19) lockdown. Thus, 1,404 participants (977 women, 426 men, and one respondent preferred not to answer) ranging from 18 to 89 years old (36.4 ± 11.7 year-old) completed a questionnaire in the period between 1st and 15th April 2021. The survey included sociodemographic data and the following validated instruments: the International Physical Activity Questionnaire (IPAQ), the Basic Need General Satisfaction Scale and the State-Trait Anxiety Inventory. The Kruskal-Wallis test was performed to examine variation in anxiety levels and BPN satisfaction according to PA category (low, moderate, and high). Spearman's Rho correlations coefficients were used to determine the association between anxiety levels and psychological needs. Individuals presenting a higher level of PA revealed lower levels of anxiety-state ( = 20.14; < 0.01). Differences between elements from different levels of PA were found for the autonomy ( = 23.52; < 0.001), competence ( = 18.89; < 0.001), and relatedness ( = 24.42; < 0.001) psychological needs, suggesting that those who feel their BPN as more satisfied have higher levels of PA. The study found statistically significant correlations between anxiety-state and the satisfaction of the needs for autonomy ( = 0.01; = -0.46), competence ( = 0.01; = -0.40), and relatedness ( = 0.01; = -0.21). These findings support the importance that PA has in the anxiety levels during social isolation, emphasizing the importance of multidisciplinary teams in an individual-based approach.
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http://dx.doi.org/10.3389/fpsyg.2021.672811DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100311PMC
April 2021

Home-Advantage during COVID-19: An Analysis in Portuguese Football League.

Int J Environ Res Public Health 2021 04 4;18(7). Epub 2021 Apr 4.

Life Quality Research Centre (CIEQV), IPSantarém/IPLeiria, 2040-413 Rio Maior, Portugal.

Covid-19 pandemic forced, at the final rounds of 2019-2020 season, in many different sport leagues worldwide, teams to play without an audience. Therefore, the present paper aims to compare the home advantage score in the last ten rounds in 2019-2020 season with the first 24 rounds in same season using Pollard's (1986) and Matos et al.'s (2020) methods. In addition, comparisons across different seasons (2016-2017; 2017-2018; 2018-2019 and 2019-2020) using the same methods were also analyzed. Results showed no differences ( > 0.05) between first 24 rounds and the last 10 in 2019-2020 season as well as in the 3 previous seasons. With Pollard's method, no differences ( > 0.05) were also found among those four seasons on global (all 34 rounds) home advantage. However, a significance difference between 2017-2018 and 2019-2020 ( < 0.05) was founded using Matos et al.'s (2020) method, which is an indicator of the importance of using complementary methods when analyzing the same realities. Overall, despite what might be expectable from recent findings, the lack of an audience in the last 10 rounds of Portuguese Football League 2019-2020 season, due to COVID-19 pandemic, did not affect home advantage. Therefore, future studies could try to analyze other different variables in Portuguese Football League, such as referees' behaviors, rules changing (e.g., the possibility of making five substitutions, instead of three), crowd dimension and density as well as include variables about odds as forecasts in football being played without crowds.
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http://dx.doi.org/10.3390/ijerph18073761DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8038430PMC
April 2021

The Relationship Between Anxiety Levels, Sleep, and Physical Activity During COVID-19 Lockdown: An Exploratory Study.

Front Psychol 2021 30;12:659599. Epub 2021 Mar 30.

Center for Innovative Care and Health Technology (ciTechCare), Polytechnic of Leiria, Leiria, Portugal.

Nowadays and worldwide, the attention is focused on coronavirus disease (COVID-19), and its consequences on mental health are yet to be fully understood. It is important to capture differences in anxiety levels among populations, groups, and the gender-related variation. Therefore, the present study had two main purposes: (1) to characterize the levels of state anxiety and trait anxiety by examining gender-related, sleep-related, and physical activity-related variations in a nonrepresentative sample of the Portuguese population during the first weeks of lockdown; and (2) to explore the possible relationship between trait anxiety and state anxiety and the possible role of gender as a moderator. This cross-sectional study comprised 1,332 Portuguese adults (aged 18-55 years old) recruited online during COVID-19 outbreak measures. Participants answered to sociodemographic data and the Portuguese version of the State-Trait Anxiety Inventory (STAI). Gender differences were found in both state anxiety ( = < 0.001; = 0.385) and trait anxiety ( = < 0.001; = 0.467) with females presenting higher values. People reporting doing more physical activity than usual during COVID-19 lockdown presented lower levels of state anxiety ( = < 0.001; = 0.200). People reporting more satisfaction with the quality of sleep presented lower levels of both state anxiety ( = < 0.001; = 0.701) and trait anxiety ( = < 0.001; = 0.899). Variation associated with the physical activity level (low, moderate, and high) was significantly different among groups in both state anxiety ( = < 0.001) and trait anxiety ( = < 0.001). When analyzing in more detail separating the levels of physical activity, participants performing moderate and high physical activity showed lower values of state and trait anxiety compared to participants with low physical activity. Participants performing high physical activity also showed lower values of state anxiety compared to participants performing moderate physical activity. Higher levels of trait anxiety were related to higher levels of state anxiety, but this association was not moderated by gender. Interventions aiming to support people psychologically during this outbreak should consider anxiety as well as gender and possible behavioral changes in sleep and physical activity, for example. Health professionals should not only consider the anxiety related to the situation we are living but also address trait anxiety to help overcome COVID-19 psychological consequences.
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http://dx.doi.org/10.3389/fpsyg.2021.659599DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8042226PMC
March 2021

Surgical outcome of a post-traumatic dystonic foot: Case report and literature review.

Foot Ankle Surg 2021 Jan 2. Epub 2021 Jan 2.

Department of Traumatology and Orthopaedics, Hospital Santa Maria, Porto, Portugal. Electronic address:

Post-traumatic dystonia is an underrecognized condition that can present with bizarre symptoms after trauma, usually out of proportion to the trigger event. We describe the case of a 31-year-old man with a severe lower extremity deformity, gradually developed after minor trauma. An interdisciplinary treatment was tried without any improvement and surgery was performed as a rescue approach. Tibialis anterior tendon transfer and hindfoot triple arthrodesis were carried out, successfully achieving a plantigrade foot and a functional gait. Despite the scarce literature available about functional results of surgery in dystonic feet, we present a step-by-step comprehensive approach to this disorder. LEVEL OF CLINICAL EVIDENCE: 4.
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http://dx.doi.org/10.1016/j.fas.2020.12.012DOI Listing
January 2021

αIIbβ3 variants in ten families with autosomal dominant macrothrombocytopenia: Expanding the mutational and clinical spectrum.

PLoS One 2020 4;15(12):e0235136. Epub 2020 Dec 4.

Unidade Multidisciplinar de Investigação Biomédica, Instituto de Ciências Biomédicas, Universidade do Porto (UMIB/ICBAS/UP), Porto, Portugal.

Background: Rare pathogenic variants in either the ITGA2B or ITGB3 genes have been linked to autosomal dominant macrothrombocytopenia associated with abnormal platelet production and function, deserving the designation of Glanzmann Thrombasthenia-Like Syndrome (GTLS) or ITGA2B/ITGB3-related thrombocytopenia.

Objectives: To describe a series of patients with familial macrothrombocytopenia and decreased expression of αIIbβ3 integrin due to defects in the ITGA2B or ITGB3 genes.

Methods: We reviewed the clinical and laboratory records of 10 Portuguese families with GTLS (33 patients and 11 unaffected relatives), including the functional and genetic defects.

Results: Patients had absent to moderate bleeding, macrothrombocytopenia, low αIIbβ3 expression, impaired platelet aggregation/ATP release to physiological agonists and low expression of activation-induced binding sites on αIIbβ3 (PAC-1) and receptor-induced binding sites on its ligand (bound fibrinogen), upon stimulation with TRAP-6 and ADP. Evidence for constitutive αIIbβ3 activation, occurred in 2 out of 9 patients from 8 families studied, but also in 2 out of 12 healthy controls. We identified 7 missense variants: 3 in ITGA2B (5 families), and 4 in ITGB3 (5 families). Three variants (αIIb: p.Arg1026Trp and p.Arg1026Gln and β3: p.Asp749His) were previously reported. The remaining (αIIb: p.Gly1007Val and β3: p.Thr746Pro, p.His748Pro and p.Arg760Cys) are new, expanding the αIIbβ3 defects associated with GTLS. The integration of the clinical and laboratory data allowed the identification of two GTLS subgroups, with distinct disease severity.

Conclusions: Previously reported ITGA2B and ITGB3 variants related to thrombocytopenia were clustered in a confined region of the membrane-proximal cytoplasmic domains, the inner membrane clasp. For the first time, variants are reported at the outer membrane clasp, at the transmembrane domain of αIIb, and at the membrane distal cytoplasmic domains of β3. This is the largest single-center series of inherited macrothrombocytopenia associated with αIIbβ3 variants published to date.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0235136PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7717987PMC
January 2021

[Thoughts about the Impact Factor].

Acta Med Port 2020 Oct 1;33(10):633-634. Epub 2020 Oct 1.

Acta Médica Portuguesa. Ordem dos Médicos. Lisboa. Portugal.

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http://dx.doi.org/10.20344/amp.14773DOI Listing
October 2020

Exploring Lifestyle Habits, Physical Activity, Anxiety and Basic Psychological Needs in a Sample of Portuguese Adults during COVID-19.

Int J Environ Res Public Health 2020 06 18;17(12). Epub 2020 Jun 18.

Department of Human Kinetics, Polytechnic Institute of Leiria, 2411 Leiria, Portugal.

This cross-sectional study aimed to characterize the lifestyle habits, anxiety levels and basic psychological needs (BPN), in Portuguese adults during the Coronavirus Disease 2019 (COVID-19) pandemic, including a comparison between genders and age groups. In total, 1404 adults (36.4 ± 11.7 years; 69.6% female) answered sociodemographic data and three instruments: the International Physical Activity Questionnaire, the Basic Need General Satisfaction Scale and the State-Trait Anxiety Inventory. Males revealed higher values for the total energy expenditure (z = -2.26; = 0.024; η 2 = 0.004) and for the level of satisfaction of competence (z = -2.62; = 0.009; η 2 = 0.005). Females showed higher scores for the anxiety state (z = -7.87; ≤ 0.001; η 2 = 0.044) and anxiety trait (z = -6.49; ≤ 0.001; η 2 = 0.030). Regarding age, higher values for the anxiety trait ( ≤ 0.001; η H 2 = 0.030) were found in the 18-34 years-old group compared to all the other age groups, also presenting significantly higher values of total energy expenditure (χ² = 13.93; = 0.008; η H 2 = 0.007) when compared to the 35-44 years-old group. Significant differences were observed between the 18-34 years-old group and the other age groups for the satisfaction of competence (χ² = 40.97; ≤ 0.001; η H 2 = 0.026), except for the >65 years-old group. Strategies for promoting well-being during periods of social isolation should consider the role of psychological dimensions and lifestyle habits according to the gender or age group.
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http://dx.doi.org/10.3390/ijerph17124360DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345948PMC
June 2020

[How Acta Médica Portuguesa Adjusted to Pandemic Times].

Acta Med Port 2020 06 31;33(6):357-358. Epub 2020 May 31.

Acta Médica Portuguesa. Ordem dos Médicos. Lisboa. Portugal.

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http://dx.doi.org/10.20344/amp.14080DOI Listing
June 2020

Coracohumeral distance and coracoid overlap as predictors of subscapularis and long head of the biceps injuries.

J Shoulder Elbow Surg 2019 Sep 20;28(9):1723-1727. Epub 2019 Apr 20.

São João University Hospital, Porto, Portugal; Porto Medical School, Porto University, Porto, Portugal.

Background: Subscapularis (SS) lesions are often underdiagnosed because of an incomplete understanding of contributing factors but also because of a greater difficulty in SS tear diagnosis with magnetic resonance imaging or physical examination. In this setting, predicting factors can be useful tools in these injuries' management. The goal of this study was to determine the influence of the coracohumeral distance (CHD) and coracoid overlap (CO) in anterior rotator cuff lesions, as well as to determine the CHD and CO values that can accurately predict SS and long head of the biceps (LHB) injuries.

Methods: We performed a retrospective, controlled, single-blinded study. We analyzed 301 patients with rotator cuff pathology and magnetic resonance imaging studies; patients with SS lesions represented the study group. The CHD and CO were measured.

Results: We found that lower CHD and higher CO values were progressively related to more serious injuries of the SS and LHB. The CHD was a very strong predictor of SS injury and tear and a good predictor of LHB injuries. A CHD of 7.6 mm had a sensitivity of 84.4% and specificity of 88.6% for SS tears. The CO was also a very strong predictor of SS tears and a good predictor of LHB injury, with a CO of 16.6 mm reaching a sensitivity of 77.8% and specificity of 68.3% for SS tears.

Conclusions: The CHD is an excellent predictor of SS tears and a good predictor of LHB lesions, with the CO also being a very strong predictor of SS tears and a good model for LHB injuries.
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http://dx.doi.org/10.1016/j.jse.2019.01.012DOI Listing
September 2019

Minimum of 10-year follow-up of V-rod technique in lumbar spondylolysis.

Eur Spine J 2019 07 26;28(7):1743-1749. Epub 2018 Nov 26.

Orthopedics Department, Centro Hospitalar São João, Porto, Portugal.

Purpose: To describe and analyze the use of the V-rod technique described by Gillet to repair spondylolysis in both early and late postoperative periods.

Methods: Patients submitted to surgical correction of lumbar spondylolysis with a V-rod system were selected upon exclusion of adjacent disk degenerative changes and high-grade spondylolisthesis. A preoperative clinical (ODI and VAS) and radiological evaluation was performed, along with assessments on the early (clinical evaluation-up to 1 year) and late (clinical and radiological-at least 10 years) postoperative periods.

Results: Twenty-two patients were included, 21 with L5 spondylolysis. Fifty percent had grade I spondylolisthesis. A significant decrease in ODI and VAS was observed from pre- to early and late post-op evaluation (all p < 0.05) but not during post-op evaluations. Changes from pre- to postoperative of both ODI and VAS were significantly higher than the minimal clinically important difference. Preoperative ODI and VAS were significantly higher in overweight/obese but similar postoperatively. No additional instability was found in late postoperative X-rays. Three patients needed revision surgery, with a survival rate of 81.8% for Gillet instrumentation at a mean follow-up of 687.7 ± 60.0 weeks.

Conclusions: Surgical treatment with V-rod system is associated with a significant improvement in ODI and VAS and radiologic stability, with an equal benefit in obese/overweight patients. This study reports for the first time an improvement that is maintained even 10 years after the initial intervention, associated with a low rate of failure. These slides can be retrieved under Electronic Supplementary Material.
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http://dx.doi.org/10.1007/s00586-018-5833-4DOI Listing
July 2019

Soluble endothelial cell molecules and circulating endothelial cells in patients with venous thromboembolism.

Blood Coagul Fibrinolysis 2017 Dec;28(8):589-595

aLaboratório de Citometria, Serviço de Hematologia Clínica, Hospital de Santo António (HSA), Centro Hospitalar do Porto (CHP)bUnidade Multidisciplinar de Investigação Biomédica, Instituto de Ciências Biomédicas Abel Salazar da Universidade do Porto (UMIB/ICBAS/UP)cSecção de Trombose e Hemostase, Serviço de Hematologia Clínica, Hospital de Santo António (HSA), Centro Hospitalar do Porto (CHP), Porto, Portugal.

: To evaluate the plasma levels of soluble endothelial cell molecules in patients with venous thromboembolism (VTE) out of the acute phase as compared with healthy individuals. We also investigated the possible associations of the soluble endothelial cell molecules among them, as well as with other clinical and laboratory data, including the numbers of circulating endothelial cells (CEC), circulating endothelial progenitor cells (CEP), and CEC expressing activation-related [cluster of differentiation (CD)54 and CD62E] and procoagulant (CD142) markers. In total, 15 patients with VTE and 20 normal individuals were studied. The CEC and CEP were quantified and characterized by flow cytometry. The soluble molecules studied included P-selectin, E-selectin, intercellular cell adhesion molecule 1, vascular cell adhesion molecule 1 and tissue factor (ELISA), and von Willebrand factor antigen (immunoturbidimetry). VTE patients had significantly higher levels of vascular cell adhesion molecule 1 and von Willebrand factor antigen and lower levels of soluble E-selectin than controls. They also showed significantly higher numbers of CEC, as of activated/procoagulant CEC and lower numbers of CEP, compared with controls. We did not find any correlation between the levels of soluble molecules and the numbers of endothelial cell in circulation, but there was with several clinical and laboratory data in VTE patients. Our results would suggest that in VTE patients, the endothelium remains activated and in some hypercoagulable state. The levels of soluble endothelial cell molecules did not seem to be directly related to the numbers of CEC and CEP neither reflected the number of activated CEC, which may be because of the different function that surface and soluble molecules may have.
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http://dx.doi.org/10.1097/MBC.0000000000000650DOI Listing
December 2017

Aneurysmal bone cyst of the lunate: Case report and literature review.

J Orthop 2016 Dec 8;13(4):352-5. Epub 2016 Jul 8.

Centro Hospitalar São João, Porto, Portugal.

The aneurysmal bone cyst (ABC) is a benign osteolytic bone neoplasm. Although most ABC is asymptomatic, in some cases they produce a mass effect, impinging against soft tissues or interfering with joint function. The authors present a rare case report of a lunate ABC successfully treated by curettage and bone grafting, and review the literature of this pathology.
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http://dx.doi.org/10.1016/j.jor.2016.06.016DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4941204PMC
December 2016

Effects of sleeping position on back pain in physically active seniors: A controlled pilot study.

Work 2015 ;53(2):235-40

LabCom - University of Beira Interio, Covilhã, Portugal.

Background: The increase in life expectancy of elderly population has aroused the interest of different knowledge areas in understanding the variables that are involved in the aging process, linking them to other concepts such as active aging, healthy aging and the bio-psycho-social changes.

Objective: This paper presents the results of the first controlled, experimental pilot study that aimed to analyze the relationship between the perception of back pain and the sleeping position adopted by physically active female seniors.

Methods: Twenty female seniors (mean age 62.70 ± 3.827) participated in this study. The individuals were separated in 2 groups (Experimental and Control Group). For the carrying out of this study, the Visual Analogue Scale (VAS) was used to measure the intensity of back pain in the spine before and after four consecutive weeks an Intervention program. Individuals in the Experimental Group were instructed regarding the recommended way to sleep position (Intervention program) according to the pathological problems or the amount of pain reported.

Results: The Experimental Group (N = 10) presented significantly (p = 0.009) fewer complaints of back pain after an Intervention program in comparison to individuals who did not receive this type of information (Control Group).
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http://dx.doi.org/10.3233/WOR-152243DOI Listing
December 2016

Circulating endothelial cells in patients with venous thromboembolism and myeloproliferative neoplasms.

PLoS One 2013 5;8(12):e81574. Epub 2013 Dec 5.

Laboratório de Citometria, Serviço de Hematologia Clínica, Hospital de Santo António (HSA), Centro Hospitalar do Porto (CHP), Porto, Portugal ; Unidade Multidisciplinar de Investigação Biomédica (UMIB), Instituto de Ciências Biomédicas Abel Salazar da Universidade do Porto (ICBAS/UP), Porto, Portugal.

Background: Circulating endothelial cells (CEC) may be a biomarker of vascular injury and pro-thrombotic tendency, while circulating endothelial progenitor cells (CEP) may be an indicator for angiogenesis and vascular remodelling. However, there is not a universally accepted standardized protocol to identify and quantify these cells and its clinical relevancy remains to be established.

Objectives: To quantify CEC and CEP in patients with venous thromboembolism (VTE) and with myeloproliferative neoplasms (MPN), to characterize the CEC for the expression of activation (CD54, CD62E) and procoagulant (CD142) markers and to investigate whether they correlate with other clinical and laboratory data.

Patients And Methods: Sixteen patients with VTE, 17 patients with MPN and 20 healthy individuals were studied. The CEC and CEP were quantified and characterized in the blood using flow cytometry, and the demographic, clinical and laboratory data were obtained from hospital records.

Results: We found the CEC counts were higher in both patient groups as compared to controls, whereas increased numbers of CEP were found only in patients with MPN. In addition, all disease groups had higher numbers of CD62E+ CEC as compared to controls, whereas only patients with VTE had increased numbers of CD142+ and CD54+ CEC. Moreover, the numbers of total and CD62+ CEC correlated positively with the white blood cells (WBC) counts in both groups of patients, while the numbers of CEP correlated positively with the WBC counts only in patients with MPN. In addition, in patients with VTE a positive correlation was found between the numbers of CD54+ CEC and the antithrombin levels, as well as between the CD142+ CEC counts and the number of thrombotic events.

Conclusions: Our study suggests that CEC counts may reveal endothelial injury in patients with VTE and MPN and that CEC may express different activation-related phenotypes depending on the disease status.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0081574PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3855326PMC
March 2015

A new iced tea base herbal beverage with Spergularia rubra extract: metabolic profile stability and in vitro enzyme inhibition.

J Agric Food Chem 2013 Sep 28;61(36):8650-6. Epub 2013 Aug 28.

REQUIMTE/Laboratório de Farmacognosia, Departamento de Química, Faculdade de Farmácia, Universidade do Porto , R. Jorge Viterbo Ferreira no. 228, 4050-313 Porto, Portugal.

Beverages are an ideal format to offer nutrients, specific health-promoting functionality, and desirable sensory attributes to consumers. Taking into account previous research on Spergularia rubra (L.) J. Presl & C. Presl, the aim of this work was to describe the chemistry and biochemistry associated with the production of a new iced tea base herbal beverage containing a hydroethanolic extract of this species, including both naturally occurring and added components. Phenolic compounds of S. rubra hydroethanolic extract and of the iced tea base herbal beverage were determined by HPLC-DAD. Thirty compounds, comprising nonacylated C-glycosyl flavones, C-glycosyl flavones acylated with aromatic acids, and C-glycosyl flavones acylated with aliphatic acids, were identified, being essentially represented by apigenin derivatives. Organic acids of both samples were determined by HPLC-UV, malic acid being the major one. A strong inhibition of α-glucosidase, acetylcholinesterase, and butyrylcholinesterase was observed. Furthermore, the influence of the pH of the digestive tube on the chemical composition of both extract and iced tea base herbal beverage and, consequently, on their biological activity, was assessed. In a general way, pH variation significantly decreased (p < 0.05) the metabolites content and enzymes inhibitory capacity. Nevertheless, the beverage enriched with S. rubra extract represents a valuable addition to consumer's health and nutrition, once the loss of activity is lower than the one verified for the base iced tea. Thus, the results suggest that the ingestion of this beverage could be of potential interest for several chronic disorders, particularly Alzheimer's disease.
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http://dx.doi.org/10.1021/jf401884uDOI Listing
September 2013

Valuable compounds in macroalgae extracts.

Food Chem 2013 Jun 28;138(2-3):1819-28. Epub 2012 Nov 28.

REQUIMTE/Laboratório de Farmacognosia, Departamento de Química, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira No 228, 4050-313 Porto, Portugal.

Bioactive compounds present in ethanolic extracts from 18 macroalgae of the Portuguese coast were analysed by gas chromatography-mass spectrometry (GC-MS), leading to the characterization of 14 compounds: proline, phloroglucinol, mannitol, 8 fatty acids and 3 sterols. A dose-dependent response against enzymes with biological significance (α-glucosidase, acetylcholinesterase and butyrylcholinesterase) and free radicals (DPPH, nitric oxide, superoxide and hydroxyl) was found, Phaeophyta being the most promising group. A PCA analysis was performed and allowed the establishment of a correlation between the algae chemical composition and the biological activity. Cystoseira tamariscifolia (Hudson) Papenfuss, Cystoseira nodicaulis (Withering) M. Roberts, Cystoseira usneoides (Linnaeus) M. Roberts and Fucus spiralis Linnaeus are among the most active species, which is in accordance with their higher contents in phloroglucinol, mannitol, oleic, arachidonic and eicosapentaenoic acids, and fucosterol. The results point to the potential interest of the use of Phaeophyta species as food additives, due to their potent antiradical activities, and especially highlights the importance of F. spiralis in the food chain of Mediterranean countries. Moreover, the incorporation of the extracts of these species in food products, nutraceutical and pharmaceutical preparations for human health should also be instigated, since they can suppress hyperglycemia and inhibit cholinesterases.
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http://dx.doi.org/10.1016/j.foodchem.2012.11.081DOI Listing
June 2013

[New perspectives on degenerative disease treatment].

Acta Reumatol Port 2009 Apr-Jun;34(2B):327-35

Serviço Ortopedia Hospital São João, Faculdade de Medicina da Universidade do Porto, Portugal.

The degenerative disc disease (DDD) is a chronic and multifactorial condition of the intervertebral disc that can manifest itself by axial pain, radiculopathy, myelopathy and spinal stenosis. It constitutes an increasing cause of lumbar pain and morbidity in Western Societies, with important socio-economic implications. The available treatment options do not modify the intervertebral disc degeneration process and they are not always capable of relieving symptoms. The loss of proteoglycan content of nucleus pulposus appears to be the main event on DDD pathophysiology. The balance between synthesis and catabolism of the extracellular matrix can be altered by the use of several proteins, including growth factors. The transfer of genes encoding these proteins is an attractive treatment option and has been successfully achieved in animal models. Moreover, the transfer of steam cells in order to repopulate the degenerated disc and to revert the pathologic process might be another promissory strategy. The authors describe the actual knowledge about DDD and the investigation that has been made in gene therapy for DDD. The comprehension of the biological mechanisms of degeneration, as well as genetic manipulation and transfer of steam cells could be decisive and revolutionary in the understanding and treatment of this epidemic condition.
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November 2009
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