Publications by authors named "Rui Liao"

100 Publications

Current Molecular Biology and Therapeutic Strategy Status and Prospects for circRNAs in HBV-Associated Hepatocellular Carcinoma.

Front Oncol 2021 2;11:697747. Epub 2021 Jul 2.

Department of Hepatobiliary Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Circular RNAs (circRNAs) are newly classified noncoding RNA (ncRNA) members with a covalently closed continuous loop structure that are involved in immune responses against hepatitis B virus (HBV) infections and play important biological roles in the occurrence and pathogenesis of HCC progression. The roles of circRNAs in HBV-associated HCC (HBV-HCC) have gained increasing attention. Substantial evidence has revealed that both tissue and circulating circRNAs may serve as potential biomarkers for diagnostic, prognostic and therapeutic purposes. So far, at least four circRNA/miRNA regulatory axes such as circRNA_101764/miR-181, circRNA_100338/miR-141-3p, circ-ARL3/miR-1305, circ-ATP5H/miR-138-5p, and several circulating circRNAs were reported to be associated with HBV-HCC development. Notably, TGF/SMAD, JAK/STAT, Notch and Wnt/β-catenin signaling pathways may play pivotal roles in this HBV-driven HCC several circRNAs. Moreover, in non-HBV HCC patients or HCC patients partially infected by HBV, numerous circRNAs have been identified to be important regulators impacting the malignant biological behavior of HCC. Furthermore, the role of circRNAs in HCC drug resistance has become a focus of research with the aim of reversing chemoresistance and immune resistance. Herein, we review the molecular biology of circRNAs in HBV-HCC and their potential in therapeutic strategies.
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http://dx.doi.org/10.3389/fonc.2021.697747DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8284075PMC
July 2021

Achieving Organic Smart Fluorophores by Controlling the Balance between Intermolecular Interactions and External Stimuli.

ACS Appl Mater Interfaces 2021 Jun 7;13(23):27491-27499. Epub 2021 Jun 7.

China Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Jiangsu National Synergistic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University (NanjingTech), 30 South Puzhu Road, Nanjing 211816, P.R. China.

Organic smart fluorophores (OSFs) are highly desirable over the past decades because of their potential applications in advanced photonic devices. However, it is still difficult and challenging to obtain such materials with tunable photophysical properties and high emission efficiency based on robust construction strategies. Therefore, we proposed a simple and efficient strategy for constructing OSFs by balancing the competition between intermolecular interactions and external stimuli via molecular structure design. In this work, four pyrene derivatives (, , , and ) with tunable stimuli-responsive properties were designed and synthesized. The tunable intermolecular interactions in solution states were successfully demonstrated by the molecular structure and solution concentration-dependent luminescence properties. The effect of alkyl chain length on molecular packing in solid states was investigated by polarized optical microscopy and powder and single-crystal X-ray diffraction; the results show that with the increase in molecular chain length, the molecular packing of the compounds gradually changed from π-π stacked compact mode to X-crossing stacked loose mode, which leads to different stimuli-responsive phenomena of these compounds. The strategy provided herein facilitates the construction of multistimuli-responsive (thermochromism, mechanochromism, and vapochromism) OSFs with adjustable emission color. Harnessing the heat-responsive luminescence properties and great solubility of , the optical information anticounterfeiting based on temperature was demonstrated by printing different concentrations of solution on filter papers. Much more, this research may provide broad implications for the design of organic smart materials based on intermolecular interactions.
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http://dx.doi.org/10.1021/acsami.1c07252DOI Listing
June 2021

Investigation of the Amide Linkages on Cooperative Supramolecular Polymerization of Organoplatinum(II) Complexes.

Molecules 2021 May 10;26(9). Epub 2021 May 10.

CAS Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, China.

Cooperative supramolecular polymerization of π-conjugated compounds into one-dimensional nanostructures has received tremendous attentions in recent years. It is commonly achieved by incorporating amide linkages into the monomeric structures, which provide hydrogen bonds for intermolecular non-covalent complexation. Herein, the effect of amide linkages is elaborately studied, by comparing supramolecular polymerization behaviors of two structurally similar monomers with the same platinum(II) acetylide cores. As compared to the -phenyl benzamide linkages, -[(1)-1-phenylethyl] benzamide linkages give rise to effective chirality transfer behaviors due to the closer distances between the chiral units and the platinum(II) acetylide core. They also provide stronger intermolecular hydrogen bonding strength, which consequently brings higher thermo-stability and enhanced gelation capability for the resulting supramolecular polymers. Supramolecular polymerization is further strengthened by varying the monomers from monotopic to ditopic structures. Hence, with the judicious modulation of structural parameters, the current study opens up new avenues for the rational design of supramolecular polymeric systems.
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http://dx.doi.org/10.3390/molecules26092832DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8126204PMC
May 2021

Description of a new species of the genus (Eulipotyphla: Talpidae: Uropsilinae) from the Dabie Mountains, Anhui, Eastern China.

Zool Res 2021 May;42(3):294-299

School of Life Science, Anhui University, Hefei, Anhui 230601, China.

During a terrestrial vertebrate survey of the Dabie Mountains in Anhui Province, eastern China, we collected four Asian shrew mole specimens (hereafter, shrew moles). Based on published literature and comparison with previously collected materials, the four specimens were similar to shrew moles from the mountains of Southwest China; however, no species in this group has been previously recorded from the Dabie Mountains. The genetic and morphological characteristics of the specimens were analyzed, based upon which a new species of shrew mole is described, named
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http://dx.doi.org/10.24272/j.issn.2095-8137.2020.266DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175959PMC
May 2021

Combined effect of silver ion and pyrite on AMD formation generated by chalcopyrite bio-dissolution.

Chemosphere 2021 Sep 9;279:130516. Epub 2021 Apr 9.

School of Minerals Processing and Bioengineering, Central South University, Changsha, Hunan, China; Key Lab of Biohydrometallurgy of Ministry of Education, Changsha, Hunan, China.

Chalcopyrite is a crucial contributor causing acid mine drainage (AMD). Silver and pyrite are commonly co-existed with chalcopyrite, and can significantly affect the copper release from chalcopyrite bio-dissolution process. However, the combined effect of them on chalcopyrite bio-dissolution has not been illustrated up to now. To fill this knowledge gap, the combined effect of silver and pyrite on chalcopyrite dissolution with Acidithiobacillus ferrooxidans was investigated in this study. The copper extraction reached the maximum value (62.3 ± 0.1%) with the presence of silver and pyrite, which was 43.8 ± 0.1% higher than the control group (without addition). This suggested more copper ions and acids were released under this circumstance. According to bio-dissolution results, SEM, XRD and XPS analyses, the promotion effect of silver and pyrite on chalcopyrite bio-dissolution was mainly attributed to the increase of ferric ions in solution and the reduction of passivation layer (S/S) on chalcopyrite surface. The investigation into the bio-dissolution of chalcopyrite is important for controlling the generation of copper ions and acids. Silver or pyrite bearing chalcopyrite should be carefully treated to avoid the pollution of heavy metal copper and acid in the mining environment.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130516DOI Listing
September 2021

LncRNA SNHG6 promotes G1/S-phase transition in hepatocellular carcinoma by impairing miR-204-5p-mediated inhibition of E2F1.

Oncogene 2021 May 6;40(18):3217-3230. Epub 2021 Apr 6.

The Second Ward of Hepatobiliary Pancreatic Surgery, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital & Chinese Academy of Sciences Sichuan Translational Medicine Research Hospital, Chengdu, PR China.

Emerging evidence suggests that long noncoding RNAs (lncRNAs) function as competitive endogenous RNA (ceRNA) targeting proteins and genes; however, the role of lncRNAs in hepatocellular carcinoma (HCC) is not well understood. We investigated the mechanism by which lncRNA SNHG6 promotes the development of HCC. RT-qPCR revealed upregulated lncRNA SNHG6 in the HCC setting. Elevated SNHG6 expression was indicative of poor prognosis in patients with HCC. SNHG6 overexpression resulted in increased cyclin D1, cyclin E1, and E2F1 expression both in vitro and in vivo. SNHG6 also promoted HCC cell proliferation by enhancing G1-S phase transition in vitro. Dual luciferase reporter assays, RIP, and RNA pull-down assays demonstrated SNHG6 competitively bound to miR-204-5p and inhibited its expression preventing miR-204-5p from targeting E2F1. Overexpression of miR-204-5p abolished the effect of SNHG6. Our data suggest that SNHG6 functions as a ceRNA that targets miR-204-5p resulting in an increased E2F1 expression and enhanced G1-S phase transition, thereby promoting the tumorigenesis of HCC.
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http://dx.doi.org/10.1038/s41388-021-01671-2DOI Listing
May 2021

The complete chloroplast genome sequence of (Gesneriaceae).

Mitochondrial DNA B Resour 2021 Mar 17;6(3):705-707. Epub 2021 Mar 17.

Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin, PR China.

Pers. is well known for its floral diversity and widely used in horticulture, but its phylogenetic position is still poorly understood. And most research about the complete chloroplast genome sequence focused on the Old World species; therefore, we think it is necessary to examine taxa of the New World in more detail. This study determined the complete chloroplast genome of H.E. Moore. The cp genome was 153,011 bp in a total length containing two inverted repeats (IRs) of 25,162 bp separated by a large single-copy (LSC) region of 84,669 bp and a small single-copy (SSC) region of 18,018 bp. The whole cp genome of contains 112 genes, including 79 protein-coding genes, 29 genes, and four genes. This plastid genome is firstly reported in the New World Gesneriaceae, which will be a valuable resource for future studies on breeding, conservation genetics, and phylogeny of Gesneriaceae.
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http://dx.doi.org/10.1080/23802359.2020.1847607DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7971201PMC
March 2021

LINC01419-mediated epigenetic silencing of ZIC1 promotes metastasis in hepatocellular carcinoma through the PI3K/Akt signaling pathway.

Lab Invest 2021 05 26;101(5):570-587. Epub 2021 Mar 26.

Chinese Academy of Sciences Sichuan Translational Medicine Research Hospital, Chengdu, PR China.

Hepatocellular carcinoma (HCC) is a rapidly growing tumor characterized by a high potential for vascular invasion and metastasis. The purpose of our study is to explore the regulation mechanism of long noncoding RNA (lncRNA) LINC01419 on cell-cycle distribution and metastasis in hepatocellular carcinoma (HCC) by regulating zinc finger of the cerebellum (ZIC1) through PI3K/Akt signaling pathway. Bioinformatics analysis and dual-luciferase reporter assay were used to analyze LINC01419 and related genes in HCC, and their expression in HCC tissues and adjacent normal tissues were determined by reverse transcription quantitative polymerase chain reaction (RT-qPCR) and western blot. Then, HCC cell lines were subjected to the construction of LINC01419/ZIC1 overexpression/knockdown cells utilizing lentiviral vectors. RIP and ChIP assays were applied to identify the LINC01419-binding protein. BSP and MSP assays were used to determine gene methylation. According to the results, LINC01419 was highly expressed in HCC tissues and cells, while ZIC1 was poorly expressed. LINC01419 targeted and downregulated ZIC1 expression. Furthermore, LINC01419 increased the methylation of ZIC1 promoter and repressed ZIC1 expression. PI3K/Akt signaling pathway was activated by LINC01419 overexpression and ZIC1 knockdown, under which conditions, the HCC cell self-renewal and proliferation were promoted while cell apoptosis was attenuated, accompanied by accelerated formation and metastasis of xenografted tumors in mice. In conclusion, LINC01419 enhances the methylation of ZIC1 promoter, inhibits ZIC1 expression, and activates the PI3K/Akt signaling pathway, thereby enhancing the malignant phenotypes of HCC cells in vitro as well as tumor formation and metastasis in vivo.
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http://dx.doi.org/10.1038/s41374-021-00539-zDOI Listing
May 2021

Integrative genomic expression analysis reveals stable differences between lung cancer and systemic sclerosis.

BMC Cancer 2021 Mar 10;21(1):259. Epub 2021 Mar 10.

Department of Rheumatology and Immunology, Shenzhen People's Hospital, The Second Clinical Medical College of Jinan University, Shenzhen, 518020, China.

Background: The incidence and mortality of lung cancer are the highest among all cancers. Patients with systemic sclerosis show a four-fold greater risk of lung cancer than the general population. However, the underlying mechanism remains poorly understood.

Methods: The expression profiles of 355 peripheral blood samples were integratedly analyzed, including 70 cases of lung cancer, 61 cases of systemic sclerosis, and 224 healthy controls. After data normalization and cleaning, differentially expressed genes (DEGs) between disease and control were obtained and deeply analyzed by bioinformatics methods. The gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed online by DAVID and KOBAS. The protein-protein interaction (PPI) networks were constructed from the STRING database.

Results: From a total of 14,191 human genes, 299 and 1644 genes were identified as DEGs in systemic sclerosis and lung cancer, respectively. Among them, 64 DEGs were overlapping, including 36 co-upregulated, 10 co-downregulated, and 18 counter-regulated DEGs. Functional and enrichment analysis showed that the two diseases had common changes in immune-related genes. The expression of innate immune response and response to virus-related genes increased significantly, while the expression of negative regulation of cell cycle-related genes decreased notably. In contrast, the expression of mitophagy regulation, chromatin binding and fatty acid metabolism-related genes showed distinct trends.

Conclusions: Stable differences and similarities between systemic sclerosis and lung cancer were revealed. In peripheral blood, enhanced innate immunity and weakened negative regulation of cell cycle may be the common mechanisms of the two diseases, which may be associated with the high risk of lung cancer in systemic sclerosis patients. On the other hand, the counter-regulated DEGs can be used as novelbiomarkers of pulmonary diseases. In addition, fat metabolism-related DEGs were consideredto be associated with clinical blood lipid data.
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http://dx.doi.org/10.1186/s12885-021-07959-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7944918PMC
March 2021

Complete chloroplast genome sequence of and (Saxifragaceae).

Mitochondrial DNA B Resour 2020 Jul 15;5(3):2837-2838. Epub 2020 Jul 15.

Hubei Provincial Key Laboratory for Protection and Application of Special Plant Germplasm in Wuling Area of China, Key Laboratory of State Ethnic Affairs Commission for Biological Technology, College of Life Science, South-Central University for Nationalities, Wuhan, China.

The complete chloroplast genome of Maxim. and L. were reported in this study. The chloroplast genomes were 153,460 bp for and 152,619 bp for . LSC and SSC of 83,670 bp and 17,342 bp were separated by two IRs of 26,224 bp each in . While contained IRs of 25,992 bp, LSC of 83,524 bp and SSC of 17,111 bp. The chloroplast genome of contains 112 unique genes, including 79 protein-coding genes, four ribosomal RNA genes, and 30 transfer RNA genes. And the chloroplast genome of contains 112 unique genes, including 79 protein-coding genes, four ribosomal RNA genes, and 30 transfer RNA genes. In addition, the rps12 gene was recognized as a trans-spliced gene and 17 intron-containing genes were also detected.
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http://dx.doi.org/10.1080/23802359.2020.1790313DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7850371PMC
July 2020

Complete chloroplast genome sequence of Schrenk from PacBio Sequel II Platform.

Mitochondrial DNA B Resour 2020 Sep 29;5(3):3452-3454. Epub 2020 Sep 29.

Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin Xinjiang Production and Construction Corps, Tarim University, Alar, China.

Schrenk plays an important role on ecological services in desert areas. The complete chloroplast genome was reported in this study using the PacBio Sequel II Platform. The chloroplast genome with a total size of 157,856 bp consists of two inverted repeats (IR, 27,673 bp) separated by a large single-copy region (LSC, 85,867 bp) and a small single-copy region (SSC, 16,645 bp). Further annotation revealed the chloroplast genome contains 111 genes, including 78 protein-coding genes, 29 tRNA genes, and 4 rRNA genes. A total of 151 simple sequence repeats (SSRs) were identified in the chloroplast genome. This information will be useful for study on the evolution and genetic diversity of in the future.
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http://dx.doi.org/10.1080/23802359.2020.1824593DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782250PMC
September 2020

Complete chloroplast genome sequence of and (Saxifragaceae).

Mitochondrial DNA B Resour 2020 May 12;5(3):2040-2041. Epub 2020 May 12.

Hubei Provincial Key Laboratory for Protection and Application of Special Plant Germplasm in Wuling Area of China, Key Laboratory of State Ethnic Affairs Commission for Biological Technology, College of Life Science, South-Central University for Nationalities, Wuhan, China.

The complete chloroplast genome of Oliv. and Fr. Schmidt. were reported in this study. The chloroplast genomes were 152,837 bp for and 151,679 bp for . LSC and SSC of 83,584 bp and 17,265 bp were separated by two IRs of 25,994 bp each in . While contained IRs of 25,973 bp, LSC of 82,772 bp and SSC of 16,961 bp, for a total 151,679 bp length. The chloroplast genome of contains 130 genes, including 85 protein-coding genes (78 PCG species), 8 ribosomal RNA genes (4 rRNA species), 37 transfer RNA genes (30 tRNA species). And the chloroplast genome of contains 130 genes, including 85 protein-coding genes (78 PCG species), 8 ribosomal RNA genes (4 rRNA species), 37 transfer RNA genes (30 tRNA species).
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http://dx.doi.org/10.1080/23802359.2020.1760152DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7782184PMC
May 2020

Ethnic disparities in stillbirth risk in Yunnan, China: a prospective cohort study, 2010-2018.

BMC Public Health 2021 01 14;21(1):136. Epub 2021 Jan 14.

State Key Laboratory of Organ Failure Research, Department of Biostatistics, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, 510515, China.

Background: Racial and ethnic disparities in stillbirth risk had been documented in most western countries, but it remains unknown in China. This study was to determine whether exist ethnic disparities in stillbirth risk in mainland China.

Methods: Pregnancy outcomes and ethnicity data were obtained from the National Free Preconception Health Examination Project (NEPHEP), a nationwide prospective population-based cohort study conducted in Yunnan China from 2010-2018. The Han majority and other four main minorities including Yi, Dai, Miao, Hani were investigated in the analysis. The stillbirth hazards were estimated by life-table analysis. The excess stillbirth risk (ESR) was computed for Chinese minorities using multivariable logistic regression.

Results: Compared with other four minorities, women in Han majority were more likely to more educated, less multiparous, and less occupied in agriculture. The pattern of stillbirth hazard of Dai women across different gestation intervals were found to be different from other ethnic groups, especially in 20-23 weeks with 3.2 times higher than Han women. The ESR of the Dai, Hani, Miao, and Yi were 45.05, 18.70, -4.17 and 12.28%, respectively. Adjusted for maternal age, education, birth order and other general risk factors, the ethnic disparity still persisted between Dai women and Han women. Adjusted for preterm birth further (gestation age <37 weeks) can reduce 16.91% ESR of Dai women and made the disparity insignificant. Maternal diseases and congenital anomalies explained little for ethnic disparities.

Conclusions: We identified the ethnic disparity in stillbirth risk between Dai women and Han women. General risk factors including sociodemographic factors and maternal diseases explained little. Considerable ethnic disparities can be attributed to preterm birth.
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http://dx.doi.org/10.1186/s12889-020-10102-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7807874PMC
January 2021

Combined transplantation of neural stem cells and bone marrow mesenchymal stem cells promotes neuronal cell survival to alleviate brain damage after cardiac arrest microRNA-133b incorporated in extracellular vesicles.

Aging (Albany NY) 2021 01 12;13(1):262-278. Epub 2021 Jan 12.

Department of Emergency, The Second Affiliated Hospital of Kunming Medical University, Kunming 650000, Yunan Province, P.R. China.

Neural stem cell (NSC) transplantation has prevailed as a promising protective strategy for cardiac arrest (CA)-induced brain damage. Surprisingly, the poor survival of neuronal cells in severe hypoxic condition restricts the utilization of this cell-based therapy. Extracellular vesicles (EVs) transfer microRNAs (miRNAs) between cells are validated as the mode for the release of several therapeutic molecules. The current study reports that the bone marrow mesenchymal stem cells (BMSCs) interact with NSCs EVs thereby affecting the survival of neuronal cells. Hypoxic injury models of neuronal cells were established using cobalt chloride, followed by co-culture with BMSCs and NSCs alone or in combination. BMSCs combined with NSCs elicited as a superior protocol to stimulate neuronal cell survival. BMSCs-derived EVs could protect neuronal cells against hypoxic injury. Silencing of miR-133b incorporated in BMSCs-derived EVs could decrease the cell viability and the number of NeuN-positive cells and increase the apoptosis in the CA rat model. BMSCs-derived EVs could transfer miR-133b to neuronal cells to activate the AKT-GSK-3β-WNT-3 signaling pathway by targeting JAK1. Our study demonstrates that NSCs promotes the release of miR-133b from BMSCs-derived EVs to promote neuronal cell survival, representing a potential therapeutic strategy for the treatment of CA-induced brain damage.
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http://dx.doi.org/10.18632/aging.103920DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835040PMC
January 2021

Removal of Horizontally Impacted Mandibular Third Molars With Large Root Bifurcations Using a Modified Tooth Sectioning Method.

J Oral Maxillofac Surg 2021 04 16;79(4):748-755.e1. Epub 2020 Dec 16.

Resident, State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Oral Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu, China. Electronic address:

Purpose: The purpose of this study was to introduce the method and first results of a modified tooth sectioning technique for the extraction of horizontally impacted mandibular third molars (M3Ms) with large root bifurcation.

Patients And Methods: A total of 300 horizontally impacted M3Ms with large root bifurcation in medically healthy patients were included in this prospective study. Patients were divided into 2 groups: the modified method group (test group), in which the M3M was sectioned between the distal root and the remainder of the tooth at the point of root bifurcation; and the conventional method group (control group), in which the M3M was sectioned between the crown and the root at the cementoenamel junction. Operation duration, postoperative reactions, complications, and patient satisfaction were analyzed and compared between the 2 groups.

Results: Each group included 150 M3Ms which were all successfully extracted. Operation durations in the test and control group were 10.48 ± 3.78 and 15.09 ± 4.24 minutes, respectively (P < .05). The test group had significantly better results than the control group with regard to postoperative reactions and complications (P < .05). Patients in the test group had higher satisfaction ratings regarding operation duration and the healing process than those in the control group (P < .05).

Conclusions: The modified method of tooth sectioning between the distal root and the remainder of the tooth can efficiently eliminate resistance from the bone and adjacent mandibular second molar and allow for just 1 sectioning of the M3M in most cases, which could make the operation straightforward and safe.
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http://dx.doi.org/10.1016/j.joms.2020.12.011DOI Listing
April 2021

IL-6 trans-signaling promotes the expansion and anti-tumor activity of CAR T cells.

Leukemia 2021 05 9;35(5):1380-1391. Epub 2020 Nov 9.

Key Laboratory of Regenerative Biology South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.

Chimeric antigen receptor (CAR) T cell therapies lead to high clinical response rates in B cell malignancies, and are under investigation for treatment of solid tumors. While high systemic interleukin- (IL-) 6 levels are associated with clinical cytokine release syndrome (CRS), the role of IL-6 trans-signaling within CAR T-cells has not been reported. We generated CAR T cells that constitutively express hyper IL-6 (HIL-6), a designer cytokine that activates the trans-signaling pathway. HIL-6-expressing CAR T-cells exhibited enhanced proliferation and antitumor efficacy in vitro and in xenograft models. However, HIL-6 CAR T cells caused severe graft-versus-host disease (GVHD). Transcriptomic profiling revealed that HIL-6 stimulation of CAR T cells upregulated genes associated with T cell migration, early memory differentiation, and IL-6/GP130/STAT3 signaling. Since IL-6 trans-signaling acts via surface GP130, we generated CAR T cells expressing a constitutively-active form of GP130 and found these retained improved antitumor activity without signs of GVHD in preclinical models of B-cell leukemia and solid tumors. Taken together, these results show that IL-6 trans-signaling can enhance expansion and antitumor activity of CAR T cells via the GP130/STAT3 pathway, and suggest that expression of GP130 within CAR T cells could lead to improved antitumor efficacy without systemic IL-6 trans-signaling.
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http://dx.doi.org/10.1038/s41375-020-01085-1DOI Listing
May 2021

Association between homocysteine and obesity: A meta-analysis.

J Evid Based Med 2020 Nov 3. Epub 2020 Nov 3.

Department of Digestive Medicine, The First Affiliated Hospital, Kunming Medical University, Kunming, Yunnan, China.

According to previous studies of obesity, we found that the association between homocysteine concentrations and obesity was reported controversially. Thus, we carried out this meta-analysis to investigate this association. We searched PubMed, Cochrane library, and EMBASE database for studies that evaluate the relationship between homocysteine concentrations and obesity from inception to March, 2019. The quality of all included studies was assessed by the Newcastle Ottawa Scale (NOS) and the Agency for Healthcare Research Quality (AHRQ). The RevMan5.3 software and Stata12.0 software were used for conducting all date analyses. Standardized mean differences (SMD) with the corresponding 95% confidence intervals (95% CIs) were used as a measure of effect size to assess the relationship between homocysteine concentrations and obesity through a meta-analysis. The level of significance was set at P < .05. A total of 14 studies were ultimately included in our meta-analysis. Meta-analysis of the 14 studies found remarkable lower homocysteine concentrations in controls than in obese patients (SMD = 0.76, 95% CI = 0.25-1.27, P < .01; I = 94% and P < .01 for heterogeneity), regardless of nutritional status, dietary habit, insulin resistance (IR) status, special disease history, history of medicine taken, genetic background, and so on. Homocysteine concentrations in nonobese patients with polycystic ovarian syndrome (PCOS) were lower than obese patients with PCOS (SMD = 0.48, 95% CI = 0.20-0.77, P < .01; I = 39% and P = .18 for heterogeneity). The result of our meta-analysis showed that homocysteine concentrations were significantly elevated among obese patients.
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http://dx.doi.org/10.1111/jebm.12412DOI Listing
November 2020

Airflow of the Two-Port Velopharyngeal Closure: Study Using Computational Fluid Dynamics.

J Craniofac Surg 2020 Nov/Dec;31(8):2188-2192

State Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Diseases, Department of Oral Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu.

Posterior pharyngeal flap palatoplasty is used to restore the function of velopharyngeal (VP) closure, after which 2 ports remain between the nasal and oral cavity. The authors hypothesized that the airflow dynamics of the upper airway is different in PPF patients compared to health subjects, who only has 1 movable port. Twenty adults who have multislice spiral computed tomography scan were included in this study. Two cylinders (radius, 2.00 mm; height, 4.5 mm) were used to recapitulate the 2-port VP structure after PPF palatoplasty. The areas of ports were modified by changing the radius of 2 cylinders. Real-time computational fluid dynamics simulation was used to capture the airflow velocity and pressures through the 2 ports. The airflow velocity and pressure of upper airway were recorded as the total areas of 2 VP ports increased. The total orifice areas of the 2-port VP closure for 4 VP conditions, including adequate closure, adequate/borderline closure, borderline/inadequate closure, and inadequate closure, were demonstrated. Significant differences between the 2-port VP function for demonstrating PPF reconstruction and the 1-port VP function were found. Airflow dynamics is dependent on the VP structure. The 2-port airflow model for mimicking VP closure after PPF palatoplasty demonstrated airflow characteristics that were significantly different from the 1-port model in normal VP closure.
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http://dx.doi.org/10.1097/SCS.0000000000006772DOI Listing
March 2021

Mesenchymal stem cell‑derived extracellular vesicles prevent neural stem cell hypoxia injury via promoting miR‑210‑3p expression.

Mol Med Rep 2020 Nov 21;22(5):3813-3821. Epub 2020 Aug 21.

Department of Emergency Internal Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, P.R. China.

Neural stem cells (NSCs) have the potential to give rise to offspring cells and hypoxic injury can impair the function of NSCs. The present study investigated the effects of mesenchymal stem cell (MSC)‑derived extracellular vesicles (EVs) on NSC injury, as well as the underlying mechanisms. MSC‑EVs were isolated and identified via morphological and particle size analysis. Cobalt chloride was used to establish a hypoxic injury model in NSCs. Terminal deoxynucleotidyl transferase dUTP nick end labeling assay was conducted to detect apoptosis. Reverse transcription‑quantitative PCR was performed to detect the expression levels of miR‑210‑3p, and western blotting was used to detect the expression levels of apoptosis‑inducing factor (AIF) and Bcl‑2 19 kDa interacting protein (BNIP3). Compared with the control group, NSC apoptosis, and the expression of miR‑210‑3p, AIF and BNIP3 were significantly higher in the cobalt chloride‑induced hypoxia group. By contrast, treatment with MSC‑EVs further increased miR‑210‑3p expression levels, but reduced NSC apoptosis and the expression levels of AIF and BNIP3 compared with the model group (P<0.05). In addition, miR‑210‑3p inhibitor reduced miR‑210‑3p expression, but promoted hypoxia‑induced apoptosis and the expression levels of AIF and BNIP3 compared with the model group (P<0.05). Collectively, the results suggested that MSC‑EVs prevented NSC hypoxia injury by promoting miR‑210‑3p expression, which might reduce AIF and BNIP3 expression levels and NSC apoptosis.
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http://dx.doi.org/10.3892/mmr.2020.11454DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7533502PMC
November 2020

A new species of (Saxifragaceae) from Shaanxi, north-western China.

PhytoKeys 2020 4;159:127-135. Epub 2020 Sep 4.

Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin 541006, China Guangxi Institute of Botany Guilin China.

Hong Liu, a new species from Shaanxi, north-western China, is described and photographed. The new species belongs to Subgen. Gamosplenium Sect. Nephrophylloides Ser. Macrophylla and is most similar to and from which it differs by having a shorter stem, rhizome absent, basal leaf absent, sterile branch arising from the flowering stem and a light yellow flower with longer stamen. A global conservation assessment is performed and classifies as Endangered (EN).
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http://dx.doi.org/10.3897/phytokeys.159.56109DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7486313PMC
September 2020

Comparison of transcatheter arterial chemoembolization with raltitrexed plus liposomal doxorubicin tegafur plus pirarubicin for unresectable hepatocellular carcinoma.

J Gastrointest Oncol 2020 Aug;11(4):747-759

Department of Hepatobiliary Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Background: There is still no general consensus on the optimal chemotherapeutic agent selection for transcatheter arterial chemoembolization (TACE) in unresectable hepatocellular carcinoma (HCC). The present study aimed to compare the efficacy and safety of TACE with raltitrexed plus liposomal doxorubicin (R + PGLD) tegafur plus pirarubicin (T + P) in patients with unresectable HCC.

Methods: A total of 148 patients with unresectable HCC treated with TACE between January 2012 and December 2016 were retrospectively analyzed. Of them, 74 patients were in the R + PGLD group and 74 patients were in the T + P group (1:1). The treatment response of the tumor, overall survival (OS) time, and adverse effects were compared between the two groups.

Results: There were no significant differences in patient characteristics or embolization effect (lipiodol deposition) between the two groups (P>0.05). R + PGLD treatment had a better clinical efficacy than T + P treatment (OR: 64.9% 45.9%, P=0.031; DC: 89.2% 74.3%, P=0.032). Portal vein invasion, hepatic vein invasion, tumor size and BCLC stage were associated with OR or DC after TACE using R + PGLD treatment. Survival analysis revealed that patients who received TACE with R + PGLD had a better prognosis than those treated with T + P. Moreover, some complications in the R + PGLD group, including vomiting, myelosuppression and cardiotoxicity, were significantly lower than those in the T + P group (P<0.05).

Conclusions: TACE with raltitrexed and liposomal doxorubicin could reduce the incidence of adverse reactions and significantly improve the OS of patients with unresectable HCC.
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http://dx.doi.org/10.21037/jgo-20-59DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7475337PMC
August 2020

Analysis of six chloroplast genomes provides insight into the evolution of Chrysosplenium (Saxifragaceae).

BMC Genomics 2020 Sep 10;21(1):621. Epub 2020 Sep 10.

Hubei Provincial Key Laboratory for Protection and Application of Special Plant Germplasm in Wuling Area of China, Key Laboratory of State Ethnic Affairs Commission for Biological Technology, College of Life Sciences, South-Central University for Nationalities, Wuhan, 430074, Hubei, China.

Background: Chrysosplenium L. (Saxifragaceae) is a genus of plants widely distributed in Northern Hemisphere and usually found in moist, shaded valleys and mountain slopes. This genus is ideal for studying plant adaptation to low light conditions. Although some progress has been made in the systematics and biogeography of Chrysosplenium, its chloroplast genome evolution remains to be investigated.

Results: To fill this gap, we sequenced the chloroplast genomes of six Chrysosplenium species and analyzed their genome structure, GC content, and nucleotide diversity. Moreover, we performed a phylogenetic analysis and calculated non-synonymous (Ka) /synonymous (Ks) substitution ratios using the combined protein-coding genes of 29 species within Saxifragales and two additional species as outgroups, as well as a pair-wise estimation for each gene within Chrysosplenium. Compared with the outgroups in Saxifragaceae, the six Chrysosplenium chloroplast genomes had lower GC contents; they also had conserved boundary regions and gene contents, as only the rpl32 gene was lost in four of the Chrysosplenium chloroplast genomes. Phylogenetic analyses suggested that the Chrysosplenium separated to two major clades (the opposite group and the alternate group). The selection pressure estimation (Ka/Ks ratios) of genes in the Chrysosplenium species showed that matK and ycf2 were subjected to positive selection.

Conclusion: This study provides genetic resources for exploring the phylogeny of Chrysosplenium and sheds light on plant adaptation to low light conditions. The lower average GC content and the lacking gene of rpl32 indicated selective pressure in their unique habitats. Different from results previously reported, our selective pressure estimation suggested that the genes related to photosynthesis (such as ycf2) were under positive selection at sites in the coding region.
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http://dx.doi.org/10.1186/s12864-020-07045-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7488271PMC
September 2020

Inhibition of B7-H4 promotes hepatocellular carcinoma cell apoptosis and autophagy through the PI3K signaling pathway.

Int Immunopharmacol 2020 Nov 14;88:106889. Epub 2020 Aug 14.

Department of Hepatobiliary Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China. Electronic address:

B7-H4 and autophagy can regulate or be induced by the PI3K signaling pathway. However, the association between B7-H4 and autophagy in hepatocellular carcinoma (HCC)remains unclear. The aim of this work was to investigate whether B7-H4 regulates autophagy via the PI3K signaling pathway in HCC cells. Here, western blotting was used to measure the expression of the related proteins involved in changes in of autophagy and apoptosis, such as LC3, P62, cleaved caspase 3, cleaved PARP, BCL-2, and BAX in Huh7 and Hep3B cells. Additionally, PI3K/AKT/mTOR signaling pathway proteins were measured. Cell counting kit-8 and flow cytometry were used to analyze the effects of B7-H4 siRNA interference on cell proliferation with the interference of B7-H4 siRNA. We found that B7-H4 siRNA increased HCC cell apoptosis and autophagy, and reduced cell proliferation. Moreover, the apoptosis-related proteins cleaved caspase 3, cleaved PARP and BAX were increased and Bcl-2 was decreased after B7-H4 siRNA interference. The expression level of the autophagy-related protein LC3Ⅱ was upregulated, while expression of the autophagy adaptor P62 expression was decreased in B7-H4 siRNA-pretreated cells. Furthermore, our data revealed that B7-H4 regulated apoptosis and autophagy through the PI3K signaling pathway in HCC cells. Therefore, these results suggested that B7-H4 plays an important role in HCC progression by affecting cell apoptosis and autophagy.
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http://dx.doi.org/10.1016/j.intimp.2020.106889DOI Listing
November 2020

[Knockdown of B7-H4/VTCN1 promotes apoptosis and autophagy of Huh7 cells by inhibiting phosphorylation of JNK].

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2020 Jul;36(7):603-608

Department of Hepatobiliary Surgery, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China. *Corresponding author, E-mail:

Objective To investigate the effects of co-stimulatory molecule B7-H4/VTCN1 on apoptosis and autophagy of hepatocellular carcinoma (HCC) cells and the potential signaling pathways. Methods After Huh7 cells were treated by B7-H4 siRNA, CCK-8 assay was used to detect the cell proliferation. Cell apoptosis was measured by flow cytometry. The protein expression levels of cleaved caspase-3 (c-caspase-3), Bcl2, LC3, P62, JNK and phosphorylated JNK (p-JNK) were examined by Western blot analysis. The autophagosome was observed by monodansylcadaverine (MDC) assay. Results After the knockdown of B7-H4, the apoptosis and autophagy of HCC cells increased, and cell proliferation decreased. Moreover, the expression levels of c-caspase-3 and LC3 II went up, while the expression levels of Bcl2 and P62 went down. Furthermore, the phosphorylation of JNK was also inhibited, and autophagosome was visible. Conclusion Knockdown of B7-H4 promotes the apoptosis and autophagy in HCC cells, which may be related to the inhibited phosphorylation of JNK.
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July 2020

Association between Aspirin and Hepatocellular Carcinoma.

N Engl J Med 2020 06;382(25):2480-2481

First Affiliated Hospital of Chongqing Medical University, Chongqing, China

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http://dx.doi.org/10.1056/NEJMc2009497DOI Listing
June 2020

Effectiveness of anti-PD-1 for hepatocellular carcinoma.

Authors:
Rui Liao

Lancet Oncol 2020 06;21(6):e293

Department of Hepatobiliary Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China. Electronic address:

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http://dx.doi.org/10.1016/S1470-2045(20)30170-4DOI Listing
June 2020

Self-efficacy Mediates Perceived Benefits and Barriers of Adherence of Heroin-dependent Patients to Methadone for Addiction Treatment: A Health Belief Model Study.

J Addict Med 2020 Jul/Aug;14(4):e110-e117

Department of Health Related Social and Behavioral Science, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, China (BY, JZ, JH, PD, SY, RL, SY); Sichuan Center for Disease Prevention and Control, Chengdu, China (YG); Anderson Cancer Center UTHealth Science Center at Houston Graduate School of Biomedical Sciences, Houston, Texas, United States of America (PG); International Initiative on Spatial Lifecourse Epidemiology (ISLE) (SY).

Objective: Although methadone for addiction treatment (MAT) has been widely used in China, the low adherence rate in MAT clinics poses a great challenge. We aimed to investigate the factors related to the adherence of heroin-dependent patients to MAT based on the Health Belief Model (HBM) in Sichuan, China.

Methods: A cross-sectional structured interview was conducted between August and November 2018. Stratified multi-stage sampling was carried out. A total of 581 participants were enrolled from 5 clinics and completed the face-to-face structured interview. Univariate, adjusted logistic regression, multivariate logistic regression analysis and the structural equation modeling (SEM) were employed to explore the association between constructs of HBM and adherence to MAT among heroin-dependent patients.

Results: The adherence rate of MAT was 79.3% in the past 6 months. Among all constructs of HBM, self-efficacy (AOR: 1.16, 95% CI: 1.10, 1.22), perceived benefits (AOR: 1.05, 95% CI: 1.00, 1.10) and perceived barriers (AOR: 0.87, 95% CI: 0.77, 0.98) were associated with adherence to MAT. Self-efficacy was directly associated with adherence to MAT (β = 0.347, P < 0.05). Perceive benefits (β = 0.276, P < 0.01) and perceived barriers (β = -0.241, P < 0.05) were directly associated with self-efficacy. However, perceived benefits (β = 0.096, P < 0.01) and perceived barriers (β = -0.084, P < 0.01) were only indirectly associated with adherence to MAT.

Conclusion: The adherence of heroin-dependent patients to MAT can be explained by self-efficacy, perceived benefits and barriers. Self-efficacy plays a significant role as a mediating variable. Future interventions should be considered to improve patients' self-efficacy to MAT.
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http://dx.doi.org/10.1097/ADM.0000000000000640DOI Listing
June 2021

Unsupervised X-ray image segmentation with task driven generative adversarial networks.

Med Image Anal 2020 05 7;62:101664. Epub 2020 Feb 7.

Siemens Healthineers, Digital Technology and Innovation, Princeton, NJ, USA. Electronic address:

Semantic parsing of anatomical structures in X-ray images is a critical task in many clinical applications. Modern methods leverage deep convolutional networks, and generally require a large amount of labeled data for model training. However, obtaining accurate pixel-wise labels on X-ray images is very challenging due to the appearance of anatomy overlaps and complex texture patterns. In comparison, labeled CT data are more accessible since organs in 3D CT scans preserve clearer structures and thus can be easily delineated. In this paper, we propose a model framework for learning automatic X-ray image parsing from labeled 3D CT scans. Specifically, a Deep Image-to-Image network (DI2I) for multi-organ segmentation is first trained on X-ray like Digitally Reconstructed Radiographs (DRRs) rendered from 3D CT volumes. Then we build a Task Driven Generative Adversarial Network (TD-GAN) to achieve simultaneous synthesis and parsing for unseen real X-ray images. The entire model pipeline does not require any annotations from the X-ray image domain. In the numerical experiments, we validate the proposed model on over 800 DRRs and 300 topograms. While the vanilla DI2I trained on DRRs without any adaptation fails completely on segmenting the topograms, the proposed model does not require any topogram labels and is able to provide a promising average dice of 86% which achieves the same level of accuracy as results from supervised training (89%). Furthermore, we also demonstrate the generality of TD-GAN through quantatitive and qualitative study on widely used public dataset.
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http://dx.doi.org/10.1016/j.media.2020.101664DOI Listing
May 2020

Catalytic effect of silver on copper release from chalcopyrite mediated by Acidithiobacillus ferrooxidans.

J Hazard Mater 2020 06 12;392:122290. Epub 2020 Feb 12.

School of Minerals Processing and Bioengineering, Central South University, Changsha, China; Key Laboratory of Biohydrometallurgy, Ministry of Education, Changsha, China.

Although silver ion in the solution is an important factor affecting the biodissolution of chalcopyrite, the effect of silver ion on the release of copper ion from chalcopyrite to the environment has not been explored until now. In order to fill this knowledge gap, the effect of silver ion on copper release from chalcopyrite in the presence of Acidithiobacillus ferrooxidans was investigated. The results indicate that silver ion significantly enhanced chalcopyrite biodissolution, thereby releasing more copper ion. In turn, this indicates that the release of copper ion from chalcopyrite to the environment was increased under these conditions. Biodissolution results, bacterial adsorption experiments, elemental composition analysis, and electrochemical analysis reveal that the enhancement of silver ion on copper ion release from chalcopyrite was mainly attributed to the improvement of electrochemical activity of chalcopyrite and the inhibition of the formation of passivation layer (S/S) on the chalcopyrite surface. This study provides a better understanding of the effect of silver ion on the release of copper ion from chalcopyrite to the environment. In the future, the influence of silver ion on chalcopyrite biodissolution should be considered in the evaluation of copper ion pollution to ensure reliability.
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http://dx.doi.org/10.1016/j.jhazmat.2020.122290DOI Listing
June 2020

Multiple organ dysfunction and rhabdomyolysis associated with moonwort poisoning: Report of four cases.

World J Clin Cases 2020 Jan;8(2):479-486

Department of Emergency Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming 650101, Yunnan Province, China.

Background: Moonwort is a widely used Chinese herbal medicine. It has various pharmacological effects, such as relieving cough and preventing asthma. To date, multiple organ dysfunction and rhabdomyolysis caused by moonwort poisoning have not been reported.

Case Summary: Here we report four cases of moonwort poisoning that presented with multiple organ dysfunction and rhabdomyolysis accompanied by vomiting, fatigue, and muscle aches. One patient was an adult male, two were adult females, and one was a boy, with an age range of 7-64 years. The adults were treated with hemoperfusion and symptomatic therapies, while the child was treated with plasma exchange and symptomatic therapies. All four patients recovered.

Conclusion: Blood purification combined with symptomatic treatment may be an effective method for managing multiple organ dysfunction and rhabdomyolysis caused by acute moonwort poisoning.
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http://dx.doi.org/10.12998/wjcc.v8.i2.479DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7000952PMC
January 2020
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