Publications by authors named "Rui Li"

3,731 Publications

  • Page 1 of 1

Domestic physical activity and new-onset hypertension: A nationwide cohort study in China.

Am J Med 2022 May 15. Epub 2022 May 15.

Division of Nephrology, Nanfang Hospital, Southern Medical University; National Clinical Research Center for Kidney Disease; State Key Laboratory of Organ Failure Research; Guangdong Provincial Institute of Nephrology, Guangdong Provincial Key Laboratory of Renal Failure Research, Guangzhou Regenerative Medicine and Health Guangdong Laboratory; Guangdong Provincial Clinical Research Center for Kidney Disease, Guangzhou, China; Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Hefei, Anhui, China; Institute of Biomedicine, Anhui Medical University, Hefei 230032, China. Electronic address:

Background: The association between domestic physical activity and the risk of hypertension remains uncertain. We aimed to evaluate the prospective relation of domestic physical activity and new-onset hypertension among Chinese males and females.

Methods: A total of 9254 adults who were free of hypertension at baseline were enrolled from the China Health and Nutrition Survey (CHNS). Data on domestic physical activity were obtained by using self-reported questionnaires, and calculated as metabolic equivalent task (MET)-hours/week. MET-hours/week may account for both intensity and time spent on activities. The study outcome was new-onset hypertension, defined as systolic blood pressure ≥140mmHg or diastolic blood pressure ≥90mmHg or diagnosed by physician or under antihypertensive treatment during the follow-up.

Results: During a median of 8.0 years (81,996 person-years) of follow-up, a total of 2892 participants developed hypertension. Overall, there were reversed J-shaped associations between the domestic physical activity and new-onset hypertension in both males and females (both P values for nonlinearity<0.001). Consistently, when domestic physical activity was assessed as categories (<10, 10-<20, 20-<30, 30-<40, 40-<50, 50-<60, 60-<70, 70-<80, 80-<90, 90-<100, ≥100 MET-hours/week), compared with those with domestic physical activity <10 MET-hours/week, the lower and similar risks of new-onset hypertension were found in participants with domestic physical activity 30-<90 MET-hours/week in both males and females.

Conclusion: Moderate domestic physical activity was associated with a lower risk of new-onset hypertension among both males and females.
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http://dx.doi.org/10.1016/j.amjmed.2022.04.023DOI Listing
May 2022

Anfibatide alleviates inflammation and apoptosis via inhibiting NF-kappaB/NLRP3 axis in ischemic stroke.

Eur J Pharmacol 2022 May 15:175032. Epub 2022 May 15.

Anhui Academy of Medical Sciences, Hefei, Anhui, 230061, China. Electronic address:

Recent evidence suggests that Nod-like receptor protein-3 (NLRP3) inflammasome is a key mediator of inflammatory response and can induce the activation of apoptosis signaling pathways in ischemic stroke. In this research, we assessed the effects of anfibatide (ANF) on inflammatory and apoptosis in cerebral ischemic injury and the potential mechanisms. Middle cerebral artery occlusion (MCAO) model was established on male Sprague-Dawley rats to induce cerebral ischemia/reperfusion (I/R) injury in vivo. Primary cortical neurons (PCN) cells were exposed to oxygen-glucose deprivation and reintroduction (OGD/R) to mimic cerebral I/R injury in vitro. The results showed that ANF markedly alleviated infarct volume, neurological deficit and neurobehavioral impairment in MCAO/R rats, enhanced cell viability and decreased LDH release in PCN after OGD/R. The number of TUNEL-positive cells, Bax, cleaved-caspase-3, p-IκBα, p-p65, NLRP3, ASC, cleaved caspase-1, IL-β and IL-18 proteins expression were significantly upregulated in the cortex of MCAO/R rats and PCN exposed to OGD/R, NLRP3 and caspase-1 mRNA levels were also evidently elevated. Bcl-2 protein expression significantly decreased in the cortex of MCAO/R rats. Treatment with ANF obviously inhibited the expression of p-IκBα, p-p65, NLRP3, ASC, cleaved caspase-1, Bax and cleaved-caspase-3, promoted the expression of Bcl-2, then decreased the TUNEL-positive cell number and the level of inflammatory cytokines (IL-β and IL-18) in cerebral ischemia reperfusion in vito and in vitro. Our findings suggest that ANF exerts effects of alleviating inflammation and apoptosis through inhibiting NF-kappaB/NLRP3 axis. ANF is a potential candidate for treating cerebral I/R injury.
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http://dx.doi.org/10.1016/j.ejphar.2022.175032DOI Listing
May 2022

Enhancement of thermal stability of proteinase K by biocompatible cholinium-based ionic liquids.

Phys Chem Chem Phys 2022 May 18. Epub 2022 May 18.

School of Physics and Astronomy, Shanghai Jiao Tong University, Shanghai 200240, China.

Proteinase K (PK) is a proteolytic enzyme that has been widely used in nucleic acid purification, leather production, environmental protection, and other industrial applications. However, this biocatalyst cannot tolerate high temperatures which has severely restricted its wider application. As reported in previous studies, cholinium-based ionic liquids (ILs) have gained tremendous attention serving as a promising media to stabilize and preserve proteins, DNA, and other biomolecules due to their environmentally benign nature and biocompatibility. In this work, we chose 13 different kinds of cholinium-based ILs to examine their effects on the thermal stability and enzymatic activity of PK. We found that biocompatible cholinium-based ions with appropriately chosen anions can greatly improve the thermal stability of PK, whose melting temperature () is increased from ∼74.4 °C to 87.7 °C. However, the enzymatic activity is slightly reduced in the presence of ILs. Further comparison of our results with other literature findings suggests that kosmotropic anions of cholinium-based ILs are crucial to maintain the thermal stability of proteins. However, to achieve the best performance, the choice of IL anions is protein specific.
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http://dx.doi.org/10.1039/d1cp04782eDOI Listing
May 2022

NF-B-Activated lncRNACASC9 Promotes Bladder Cancer Progression by Regulating the TK1 Expression.

J Oncol 2022 7;2022:9905776. Epub 2022 May 7.

Medical College of Soochow University, Suzhou, 215123 Jiangsu, China.

Long noncoding RNAs (lncRNA) are involved in cancer development, but the roles of most lncRNAs are undocumented. In this study, we identified lncRNAs that were abnormally expressed in bladder cancer. We found that lncRNACASC9 plays an important role in the progression of bladder cancer. CASC9 was highly expressed in bladder cancer cells and tissues, and the prognosis of bladder cancer patients with high expression of CASC9 was poor. The results of colony formation assays, CCK-8 assays, EdU assays, transwell assays, mouse xenograft models, and tail vein injection lung metastasis model showed that CASC9 could promote bladder cancer cells growth and metastasis both in vitro and in vivo. Mechanistically, through FISH experiments, luciferase reporter experiments, and RIP experiments, we proved that CASC9 regulated the expression of TK1 by adsorbing miR-195-5p, thereby exerting an oncogenic effect in bladder cancer. Taken together, our findings support that the CASC9/miR-195-5p/TK1 axis is a critical pathway in the tumorigenesis and progression of bladder cancer, implicating a new therapeutic direction for the treatment of bladder cancer.
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http://dx.doi.org/10.1155/2022/9905776DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9107360PMC
May 2022

A Survey of Multifingered Robotic Manipulation: Biological Results, Structural Evolvements, and Learning Methods.

Front Neurorobot 2022 27;16:843267. Epub 2022 Apr 27.

State Key Laboratory for Management and Control of Complex Systems, Institute of Automation, Chinese Academy of Sciences, Beijing, China.

Multifingered robotic hands (usually referred to as dexterous hands) are designed to achieve human-level or human-like manipulations for robots or as prostheses for the disabled. The research dates back 30 years ago, yet, there remain great challenges to effectively design and control them due to their high dimensionality of configuration, frequently switched interaction modes, and various task generalization requirements. This article aims to give a brief overview of multifingered robotic manipulation from three aspects: a) the biological results, b) the structural evolvements, and c) the learning methods, and discuss potential future directions. First, we investigate the structure and principle of hand-centered visual sensing, tactile sensing, and motor control and related behavioral results. Then, we review several typical multifingered dexterous hands from task scenarios, actuation mechanisms, and in-hand sensors points. Third, we report the recent progress of various learning-based multifingered manipulation methods, including but not limited to reinforcement learning, imitation learning, and other sub-class methods. The article concludes with open issues and our thoughts on future directions.
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http://dx.doi.org/10.3389/fnbot.2022.843267DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097019PMC
April 2022

Complete chloroplast genome of and its phylogenetic position in Plantaginaceae.

Mitochondrial DNA B Resour 2022 10;7(5):819-821. Epub 2022 May 10.

School of Life Sciences, Key Laboratory of Plant Stress Biology, Henan University, Kaifeng, China.

, an herbaceous perennial species of Plantaginaceae, has been used as a traditional herbal medicine plant in China. In this study, the complete chloroplast (cp) genome of was sequenced and assembled using genome skimming data. The cp genome was 165,045 bp in length including the large single-copy (LSC, 82,964 bp) and small single-copy (SSC, 4,633 bp) regions separated by two copies of inverted region (IR, 38,724 bp). The cp genome encoded 113 unique genes, consisting of 79 protein-coding genes, 30 tRNA genes, and four rRNA genes, additionally with 27 duplicated genes in the IR regions. Phylogenetic analysis indicated that the representative species from was monophyletic and they were divided into four subgenera. belongs to the subgenus and was sister to with high bootstrap value support.
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http://dx.doi.org/10.1080/23802359.2022.2073838DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9103697PMC
May 2022

Low-Field NMR Experimental Study on the Effect of Confining Pressure on the Porous Structure and Connectivity of High-Rank Coal.

ACS Omega 2022 Apr 12;7(16):14283-14290. Epub 2022 Apr 12.

School of Safety and Management Engineering, Hunan Institute of Technology, Hengyang, Hunan 421002, China.

To study the influence of different confining pressures on the pore structure and connectivity of high-rank coal, the high-rank raw coal of the Shanxi Xinjing Mine No. 9 coal seam was studied. A low-field nuclear magnetic resonance (LNMR) test system and a vacuum pressurized water saturation system were used to analyze the samples. The spectra of samples, saturated with water under different confining pressures and containing residual water after centrifugation, were tested. The coal sample pore size distributions, permeabilities, free fluid values, bound fluid values, and other parameters were obtained, and a calculation model of the coal pore connectivity ratio was established. The results were as follows. When the saturated pressures were 5, 10, 15, 20, 25, and 30 MPa, the pore diameters of the coal samples were mainly concentrated in the ranges of 0.00023-0.069 and 1.29-24.09 μm. Among them, micropores (<10 nm) and small pores (10 < 100 nm) account for the main part, mesopores (100 < 1000 nm) were underdeveloped, and relatively few macropores (>1000 nm) and fissures developed. As the confining pressure increased, the coal porosity and connectivity showed a trend of decreasing, then increasing, and finally remaining basically unchanged. The total pore connectivity rates of the coal samples were 37.0-62.6%. The interconnection rates of the micropores, small holes, mesopores, and macropores are 2.90-34.55, 89.09-99.03, 97.09-100, and 100%, respectively. The total pore connectivity followed an exponential functional relationship with permeability, and the critical confining pressure of high-rank coal was 25 MPa. These results provide a scientific basis for the high-pressure water injection of high-rank coal seams.
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http://dx.doi.org/10.1021/acsomega.2c01154DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9089745PMC
April 2022

The Value of Contrast-Enhanced Ultrasound in the Evaluation of Carotid Web.

Front Neurol 2022 27;13:860979. Epub 2022 Apr 27.

Stroke Center & Department of Neurology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China.

Objectives: The purpose of this study was to investigate whether contrast-enhanced ultrasound (CEUS) is more advantageous than conventional ultrasound in the diagnosis of carotid web (CaW) and to compare the clinical characteristics of patients in different age groups.

Methods: Seventeen patients admitted to the hospital from October 2019 to December 2021 were included in our study. Patients were initially diagnosed with CaW using digital subtraction angiography (DSA), and conventional ultrasound and CEUS were completed. Baseline patient data were analyzed and compared between the <60 years old CaW group and the ≥60 years old CaW group to explore the differences between the two groups. Then, comparing the accuracy of conventional ultrasound and CEUS.

Results: A total of 17 CaW patients participated in this study, including 4 female patients (23.5%) and 13 male patients (76.5%), with an average age of 59.41 (±10.86) years. There were 9 patients (52.9%) with left CaW and 8 patients (47.1%) with right CaW. Acute ischemic stroke (AIS) occurred in 14 patients (82.4%). Thrombosis occurred in five of 17 patients (29.4%). There was a significant statistical difference about the thrombosis between the <60 years old CaW group and the ≥60 years old CaW group [<60 years group: 0 (0%), ≥60 years group: 5 (62.5%), = 0.005]. Seven patients (41.2%) received medical management, nine patients (52.9%) had carotid artery stenting (CAS), and one patient (5.9%) had carotid endarterectomy (CEA). None of the patients had recurrent stroke during the follow-up period. The diagnostic rate of CaW and thrombus by CEUS was higher than that by conventional ultrasound, and there was a significant statistical difference in the diagnosis of thrombus between CEUS and conventional ultrasound (χ2 = 4.286, = 0.038).

Conclusions: CEUS may have a higher diagnostic accuracy for CaW with thrombosis, and it has a higher clinical application prospect.
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http://dx.doi.org/10.3389/fneur.2022.860979DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9093455PMC
April 2022

Repeatability of Ultrasound-Defined Bladder Shape Metrics in Healthy Volunteers.

Res Rep Urol 2022 6;14:185-192. Epub 2022 May 6.

Department of Mechanical and Nuclear Engineering, Virginia Commonwealth University College of Engineering, Richmond, VA, USA.

Purpose: Recent studies demonstrate the potential value of using non-invasive abdominal ultrasound to quantify bladder shape and its association with disorders of bladder filling and voiding. The aim of the present study was to assess the repeatability of ultrasound-defined bladder shape metrics within the same individual from multiple bladder fills and study visits.

Patients And Methods: Healthy volunteers completed an oral hydration protocol with two weekly visits, each with two consecutive bladder fill-void cycles, providing four total fills per participant. Throughout filling, 3-dimensional (3-D) ultrasound images were recorded at 5-minute intervals. Perimeters were manually traced in six cross-sectional planes 30° apart using GE 4D-View software. Diameters, perimeters and cross-sectional areas for the transverse, sagittal and coronal planes were interpolated at three standard bladder volumes of 200, 300, and 400mL. To quantify repeatability, intraclass correlation coefficients (ICCs) and normalized mean absolute differences were calculated for each metric at each standard volume. Moderate and good repeatability were defined as ICC ≥0.5 and ICC ≥0.75, respectively.

Results: Data from 16 healthy volunteers (9 females, 7 males) were analyzed. ICCs for the transverse vertical and horizontal diameters showed good repeatability, and five of nine perimeter ICCs showed moderate or good repeatability. The mean absolute difference/mean ratio was ≤4% for all perimeter measurements, indicating repeatability was consistent for multiple fills and visits.

Conclusion: Initial evidence indicates that bladder shape metrics are relatively repeatable and therefore feasible to pursue as a non-invasive tool for potential evaluation of bladder function.
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http://dx.doi.org/10.2147/RRU.S351347DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9091689PMC
May 2022

Oxidation Behavior of the Si-B-X (X = Mo, Cr, or Ti) Alloys in the Temperature Range of 1000-1400 °C.

ACS Omega 2022 May 21;7(17):15145-15157. Epub 2022 Apr 21.

State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, China.

Low-melting-point silicon-boron system alloys are promising for low-temperature reactive melt infiltration to reduce high-temperature damage to silicon carbide fibers during the densification of SiC/SiC composites. Meanwhile, the oxidation resistance of the alloys will have a large impact on the intrinsic oxidation resistance of the composite. Herein, three alloys, Si-14.88B-7Mo, Si-14.88B-7Ti, and Si-14.88B-7Cr, were fabricated to investigate the oxidation behavior in air at 1000-1400 °C. The results showed that the oxidation weight gains of the Si-B-Mo alloy after oxidation at 1400 °C for 100 h were 0.9 mg/cm, which were only 50 and 1.5% of those of Si-B-Ti and Si-B-Cr alloys, respectively. The excellent oxidation resistance of Si-B-Mo alloys at 1000-1400 °C was attributed to the formation of glassy-surface layers and the dense internal oxide layer. The dense oxide layer and the low solubility of Mo ions in SiO inhibit the volatilization of MoO and the oxidation reaction, reducing the oxidation rate of the Si-B-Mo alloy. The difference in the coefficients of thermal expansion for SiO and TiO led to penetrating cracks in the oxide layer of the Si-B-Ti alloy during cooling, thereby reducing the oxidation resistance. In addition, the rate of volatilization of CrO as CrO in an oxidation atmosphere above 1200 °C increased significantly in the Si-B-Cr alloy. The simultaneous volatilization of BO and CrO resulted in the formation of loose oxide layers in the CrB region of the Si-B-Cr alloy, leading to severe oxidation.
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http://dx.doi.org/10.1021/acsomega.2c01085DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9089750PMC
May 2022

Characteristics of Bacterial Microbiota in Different Intestinal Segments of Aohan Fine-Wool Sheep.

Front Microbiol 2022 28;13:874536. Epub 2022 Apr 28.

Key Laboratory of Animal Genetics, Breeding, and Reproduction of the Ministry of Agriculture and Beijing Key Laboratory of Animal Genetic Improvement, China Agricultural University, Beijing, China.

The microbial community performs vital functions in the intestinal system of animals. Modulation of the gut microbiota structure can indirectly or directly affect gut health and host metabolism. Aohan fine-wool sheep grow in semi-desert grasslands in China and show excellent stress tolerance. In this study, we amplified 16S rRNA gene to investigate the dynamic distribution and adaptability of the gut microbiome in the duodenum, jejunum, ileum, cecum, colon, and rectum of seven Aohan fine-wool sheep at 12 months. The results showed that the microbial composition and diversity of the ileum and the large intestine (collectively termed the hindgut) were close together, and the genetic distance and functional projections between them were similar. Meanwhile, the diversity index results revealed that the bacterial richness and diversity of the hindgut were significantly higher than those of the foregut. We found that from the foregut to the hindgut, the dominant bacteria changed from Proteobacteria to Bacteroidetes. In LEfSe analysis, was found to be significantly abundant bacteria in the foregut and was involved in succinic acid metabolism. and were significantly abundant in hindgut, which can degrade cellulose polysaccharides in the large intestine and produce beneficial metabolites. Moreover, and are involved in flavonoid metabolism and polyphenol production. Interestingly, these unique bacteria have not been reported in Mongolian sheep or other sheep breeds. Collectively, the gut microbiota of Aohan fine-wool sheep is one of the keys to adapting to the semi-desert grassland environment. Our results provide new insights into the role of gut microbiota in improving stress tolerance and gut health in sheep.
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http://dx.doi.org/10.3389/fmicb.2022.874536DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9097873PMC
April 2022

Plasma glucose levels and diabetes are independent predictors for mortality in patients with COVID-19.

Epidemiol Infect 2022 May 16:1-26. Epub 2022 May 16.

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, P. R. China.

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http://dx.doi.org/10.1017/S095026882200022XDOI Listing
May 2022

Thermal properties and pyrolysis kinetics of phosphate-rock acid-insoluble residues.

Waste Manag 2022 May 12;146:77-85. Epub 2022 May 12.

School of Chemistry and Chemical Engineering, Guizhou University, Guiyang 550025, China. Electronic address:

In the phosphorous-sulphur two-step process for the clean production of phosphoric acid, a phosphate-rock acid-insoluble residue (PAIR) is a solid filter residue obtained via the phosphoric acid acidolysis of phosphate rock (PR). PAIR combined with other raw materials can be used to prepare cement, ceramics and glasses, opening a potential avenue for large-scale PAIR utilisation. However, the preparation of such materials requires high-temperatures calcination. Understanding the high-temperature thermal properties of PAIR can enable its more targeted comprehensive utilisation or disposal. In this study, the thermal properties and pyrolysis kinetics of PAIR were systematically studied using a multiple heating rate method based on thermogravimetric analysis and a kinetic model. Results showed that from room temperature to 1200 °C, the main changes in the PAIR were the complete removal of fluorine and sulphur, partial removal of phosphorus and conversion of quartz to cristobalite. Moreover, during these processes, HO(g), NH, N, CO, SO, PO(g), CO, CF and organic gases were volatilised. Herein, the pyrolysis kinetics of PAIR is divided into five stages. Stage 1 (conversion rate ɑ: 0.05-0.2) conforms to the random nucleation and growth as well as the Avrami-Erofeev (n = 2/3) mechanism; the corresponding mechanism function is F(ɑ) = [-Ln(1 - ɑ)]. Stage 2 (ɑ: 0.2-0.4) conforms to the first-order chemical reaction mechanism; the corresponding mechanism function is F(ɑ) = -Ln(1 - ɑ). Stage 3 (ɑ: 0.4-0.6) conforms to the phase boundary-controlled reaction and one-dimensional movement mechanism; the corresponding mechanism function is F(ɑ) = ɑ. Stage 4 (ɑ: 0.6-0.8) conforms to the three-dimensional diffusion process (Jander model); the corresponding mechanism function is F(ɑ) = [1 - (1 - ɑ)]. Stage 5 (ɑ: 0.6-0.95) conforms to the one-dimensional diffusion process; the corresponding mechanism function is F(ɑ) = ɑ.
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http://dx.doi.org/10.1016/j.wasman.2022.04.039DOI Listing
May 2022

Successions and interactions of phyllospheric microbiome in response to NH exposure.

Sci Total Environ 2022 May 10:155805. Epub 2022 May 10.

Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; Sino-Danish College, University of Chinese Academy of Sciences, Beijing 100049, China; College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China; Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, China.

Phyllosphere and numerous phyllospheric microbiomes present a huge potential for air pollution mitigation. Despite research investigating the microbial compositions in the phyllosphere, the successions and interactions of the phyllospheric microbiome under ammonia gas (NH) stress remain poorly understood. Herein, we performed 16S rDNA, the internal transcribed spacer (ITS) profiling and a quantitative microbial element cycling (QMEC) method to reveal successions, co-occurrence, and N-cycling functions changes of phyllospheric bacteria and fungi during NH exposure. The NH input mainly elevated ammonium (NH-N) and total nitrogen (TN) levels on the leaf surface. The exposure in the phyllosphere decreased fungal concentration with a homogeneity increase while enhanced bacterial concentration with a noticeable richness drop. Both short-term (2-week) and long-term (6-week) exposure induced significant changes in microbial compositions. Bacterial genera (Nocardioides, Pseudonocardia) and fungal genera (Alternaria, Acremonium) dominated throughout the exposure. Intensive microbial interactions compared to that in the natural phyllosphere were observed via network analysis. Our results showed that N-cycling functional genes were largely stimulated by the exposure and might, in turn contribute to NH pollution buffer and alleviation via microbial metabolism. This study extended the knowledge on microbial responses to NH exposure in the phyllosphere and enlightened phylloremediation on NH through the microbial role.
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http://dx.doi.org/10.1016/j.scitotenv.2022.155805DOI Listing
May 2022

Characterization of the complete chloroplast genome of (Saxifragaceae).

Mitochondrial DNA B Resour 2022 3;7(5):741-743. Epub 2022 May 3.

Key laboratory of Plant Stress Biology, School of Life Sciences, Henan University, Kaifeng, China.

, which is distributed in Japan and China, is the type species of . In this study, we sequenced and assembled the complete chloroplast (cp) genome of and reconstructed the phylogeny of Saxifragaceae based on the whole cp genome sequences. The cp genome of was 156,274 bp in length, comprising a pair of inverted repeat regions (25,099 bp) separated by a large single copy region (88,109 bp) and a small single copy region (17,967 bp). The genome encoded 112 unique genes consisting of 78 different protein-coding genes, 30 transfer RNA and four ribosomal RNA genes, with 16 duplicated genes in the inverted repeats. Phylogenetic analysis indicated that together with three species formed a high support clade, which was sister to .
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http://dx.doi.org/10.1080/23802359.2022.2070040DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9090422PMC
May 2022

Preoperative Nomogram and Risk Calculator for Postoperative Hypoxemia and Related Clinical Outcomes Following Stanford Type A Acute Aortic Dissection Surgery.

Front Cardiovasc Med 2022 25;9:851447. Epub 2022 Apr 25.

Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: Hypoxemia is a common complication after Stanford type A acute aortic dissection surgery (AADS), however, few studies about hypoxemia after AADS exist. The aims of this study were to identify independent risk factors for hypoxemia after AADS and to clarify its association with clinical outcomes.

Methods: Patients undergoing AADS from 2016 to 2019 in our hospital were identified and used as a training set. Preoperative variables were first screened by univariate analysis and then entered into a multivariate logistic regression analysis to identify independent risk factors. A nomogram and an online risk calculator were constructed based on the logistic model to facilitate clinical practice and was externally validated in an independent dataset.

Results: Severe hypoxemia developed in 119 of the 492 included patients (24.2%) and poorer clinical outcomes were observed in these patients. Five independent risk factors for severe hypoxemia after AADS were identified by multivariate analysis, including older age, smoking history, renal insufficiency, higher body mass index, and white blood cell count. The model showed good calibration, discrimination, and clinical utility in the training set, and was well validated in the validation set. Risk stratification was performed and three risk groups were defined as low, medium, and high risk groups. Hypertension was identified as an independent risk factor for moderate hypoxemia besides the five predictors mentioned above, and renal insufficiency was not significant for mild hypoxemia by multivariate analysis. In addition, although frozen elephant trunk was associated with increased risk of postoperative hypoxemia in the univariate analysis, frozen elephant trunk was also not identified as an independent risk factor for postoperative hypoxemia in the multivariate analysis.

Conclusion: Hypoxemia was frequent following AADS, related to poorer clinical outcomes. Predictors were identified and a nomogram as well as an online risk calculator predicting severe hypoxemia after AADS was developed and validated, which may be helpful for risk estimation and perioperative management.
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http://dx.doi.org/10.3389/fcvm.2022.851447DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9082545PMC
April 2022

Rice washing drainage (RWD) embedded in poly(vinyl alcohol)/sodium alginate as denitrification inoculum for high nitrate removal rate with low biodiversity.

Bioresour Technol 2022 May 12;355:127288. Epub 2022 May 12.

School of Water Resources and Environment, China University of Geosciences (Beijing), Beijing 100083, China. Electronic address:

Immobilization technology with low maintenance is a promising alternative to enhance nitrate removal from water. In this study, washing rice drainage (RWD) was immobilized by poly(vinyl alcohol)/sodium alginate (PVA/SA) to obtain RWD-PVA/SA gel beads as inoculum for denitrification. When initial nitrate concentration was 50 mg N/L, nitrate was effectively removed at rates of 50-600 mg/(L∙d) using acetate as carbon source (C/N = 1.25). Arrhenius activation energy (Ea) of nitrate oxidoreductase was 28.64 kJ/mol for the RWD-PVA/SA gel beads. Temporal and spatial variation in microbial community structures were revealed along with RWD storage and in the reactors by Illumina high-throughput sequencing technology. RWD-PVA/SA gel beads has a simple (operational taxonomic units (OTUs) 〈100). Dechloromonas, Pseudomonas, Flavobacterium and Acidovorax were the most four dominant genera in the denitrification reactors inoculated with RWD-PVA/SA gel beads. This study provides an inoculum for denitrification with high nitrate removal performance and simple microbial community structures.
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http://dx.doi.org/10.1016/j.biortech.2022.127288DOI Listing
May 2022

Serum immunoglobulin profiles in Chinese children with Henoch-Schönlein purpura.

Scand J Immunol 2022 May 10:e13191. Epub 2022 May 10.

Department of Pediatrics, The First Affiliated Hospital of Anhui Medical University, Hefei, People's Republic of China.

The present study focuses on the associations of serum immunoglobulin with disease activity, relapse/recurrence and renal involvement in Henoch-Schönlein purpura (HSP) children. 1683 Chinese children with HSP were recruited from January 2015 to January 2021. Laboratory data of blood samples and urine tests were collected. Renal biopsy was performed by the percutaneous technique. Patients' histories associated with HSP onset were obtained by interviews and questionnaires. (1) IgA and IgE were dramatically elevated in HSP patients as compared to their normal values and subject to a 1.75-3.09 fold and a 1.97-2.61 fold increase, respectively. (2) No significant correlation of the disease activity with IgA and IgE was determined, respectively. (3) The serum IgA levels were significantly lower in patients with relapse/recurrence than that in patients without relapse/recurrence, which may be attributed to the transmission of IgA-mediated immune complexes from blood to vessel walls. (4) No significant correlation was found between serum IgA, IgE levels and the pathological classification. HSP children have marked disorders of serum immunoglobulin profiles, characterized by significant increases in IgA and IgE. The detection of serum IgA may be applicable to predict relapse/recurrence of HSP whereas not associated with disease activity and renal involvement.
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http://dx.doi.org/10.1111/sji.13191DOI Listing
May 2022

Whole-genome sequencing analysis of a rare Salmonella diarizonae clinical strain carrying multiple plasmids and novel gene cassettes.

J Glob Antimicrob Resist 2022 May 5. Epub 2022 May 5.

College of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430023, China. Electronic address:

Objectives: Human infection caused by an uncommon Salmonella enterica subsp. diarizonae (hereafter S. diarizonae) is rising. However, knowledge concerning S. diarizonae is still limited. This study aimed to investigate the genomic features of S. diarizonae S499 isolated from a child patient with gastroenteritis symptom in China.

Methods: The antimicrobial susceptibility of S. diarizonae S499 was determined by microdilution broth assay. Whole genome was sequenced using Illumina HiSeq X-10 and PacBio RS II platforms and was de novo assembled using Unicycler and SPAdes. Conjugation experiment was performed by a broth mating method.

Results: S. diarizonae S499 was a multi-drug resistance (MDR) isolate and showed resistance to all cephalosporin drugs tested. Six plasmids (pS0499A, pS0499B, pS0499C, pS0499D, pS0499E and pS0499F) were identified. A rare gene cassette IS26-bla-wbuc-△bla-IS26-intI1 was repeatedly inserted into pS0499A for three times in one locus and reversely inserted into plasmid pS0499D. That enhanced cephalosporin resistance. To the best of our knowledge, this finding has not been reported previously. Both pS0499A and pS0499B contained multiple resistance genes and could transfer to recipient strain E. coli EC600.

Conclusion: This paper reported the genome features of S. diarizonae S499, which contained four resistant plasmids including a novel plasmid pS0499A with a novel gene cassette rearrangement. These data could contribute to a better understanding of the antimicrobial resistance mechanisms and transmission dynamics of S. diarizonae.
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http://dx.doi.org/10.1016/j.jgar.2022.04.026DOI Listing
May 2022

Spatiotemporal mapping of gene expression landscapes and developmental trajectories during zebrafish embryogenesis.

Dev Cell 2022 Apr 22. Epub 2022 Apr 22.

BGI-Shenzhen, Shenzhen 518083, China; Shenzhen Key Laboratory of Single-Cell Omics, Shenzhen 518083, China. Electronic address:

A major challenge in understanding vertebrate embryogenesis is the lack of topographical transcriptomic information that can help correlate microenvironmental cues within the hierarchy of cell-fate decisions. Here, we employed Stereo-seq to profile 91 zebrafish embryo sections covering six critical time points during the first 24 h of development, obtaining a total of 152,977 spots at a resolution of 10 × 10 × 15 μm (close to cellular size) with spatial coordinates. Meanwhile, we identified spatial modules and co-varying genes for specific tissue organizations. By performing the integrated analysis of the Stereo-seq and scRNA-seq data from each time point, we reconstructed the spatially resolved developmental trajectories of cell-fate transitions and molecular changes during zebrafish embryogenesis. We further investigated the spatial distribution of ligand-receptor pairs and identified potentially important interactions during zebrafish embryo development. Our study constitutes a fundamental reference for further studies aiming to understand vertebrate development.
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http://dx.doi.org/10.1016/j.devcel.2022.04.009DOI Listing
April 2022

Immunotherapeutic Significance of a Prognostic Alternative Splicing Signature in Bladder Cancer.

Technol Cancer Res Treat 2022 Jan-Dec;21:15330338221090093

The Second Affiliated Hospital, Hengyang Medical School, 34706University of South China, Hengyang, China.

Bladder cancer is the fourth most common malignancy in men in the United States. Aberrant alternative splicing (AS) events are involved in the carcinogenesis, but the association between AS and bladder cancer remains unclear. This study aimed to construct an AS-based prognostic signature and elucidate the role of the tumor immune microenvironment (TIME) and the response to immunotherapy and chemotherapy in bladder cancer. Univariate Cox regression analysis was performed to detect prognosis-related AS events. The least absolute shrinkage and selection operator (LASSO) and multivariate Cox analyses were employed to build prognostic signatures. Kaplan-Meier survival analysis, multivariate Cox regression analysis, and receiver operating characteristic (ROC) curves were conducted to validate the prognostic signatures. Then, the Estimation of Stromal and Immune cells in MAlignant Tumor tissues using Expression data (ESTIMATE) and tumor immune estimation resource (TIMER) databases were searched and the single-sample gene set enrichment analysis (ssGSEA) algorithm and CIBERSORT method were performed to uncover the context of TIME in bladder cancer. The Tumor Immune Dysfunction and Exclusion (TIDE) web tool and pRRophetic algorithm were used to predict the response to immunotherapy and chemotherapy. Finally, we constructed a correlation network between splicing factors (SFs) and survival-related AS events. A total of 4684 AS events were significantly associated with overall survival in patients with bladder cancer. Eight prognostic signatures of bladder cancer were established, and a clinical survival prediction model was built. In addition, the consolidated prognostic signature was closely related to immune infiltration and the response to immunotherapy and chemotherapy. Furthermore, the correlation identified EIF3A, DDX21, SDE2, TNPO1, and RNF40 as hub SFs, and function analysis found ubiquitin-mediated proteolysis is correlated most significantly with survival-associated AS events. Our findings highlight the prognostic value of AS for patients with bladder cancer and reveal pivotal players of AS events in the context of TIME and the response to immunotherapy and chemotherapy, which may be important for patient management and treatment.
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http://dx.doi.org/10.1177/15330338221090093DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9083046PMC
May 2022

The apple receptor-like kinase MdSRLK3 positively regulates resistance against pathogenic fungus by affecting the Ca signaling pathway.

Phytopathology 2022 May 4. Epub 2022 May 4.

Northwest Agriculture and Forestry University, 12469, plant pathology, No.3 Taicheng Road, Yangling, China, 712100;

is the main pathogenic fungus that causes the apple canker, a destructive disease severely threatening apple production in the world. However, the underlying key components involved in resistance against in apple trees remain largely unexplored. Here, we isolated and functionally characterized a G-type Lectin S-receptor-like protein kinase MdSRLK3 from the cultivar Royal Gala derivative line GL-3. qRT-PCR showed that the relative expression of in apple branches reached its highest level at 24 hours post inoculation, which was 13.42 times higher than without inoculation. Transient overexpression of enhanced apple resistance against , while transient silencing of reduced its resistance against the pathogen. More importantly, stable silencing of resulted in reduced resistance against this fungus. Furthermore, we demonstrated that positively regulated apple resistance by affecting the Ca signaling pathway, and the regulation was also related to the HO and callose signaling pathways. Overall, our data reveals that is a positive regulator of apple immunity.
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http://dx.doi.org/10.1094/PHYTO-11-21-0471-RDOI Listing
May 2022

Application of biochar immobilized microorganisms for pollutants removal from wastewater: A review.

Sci Total Environ 2022 Apr 30;837:155563. Epub 2022 Apr 30.

College of Environmental Science and Engineering, Yangzhou University, Yangzhou, Jiangsu 225127, China.

Microbial immobilization technology (MIT) has been rapidly developed and used to remove pollutants from water/wastewater in recent years, owing to its high stability, rapid reaction rate, and high activity. Microbial immobilization carrier with low cost and high removal efficiency is the key of MIT. Biochar is considered to be an efficient carrier for microbial immobilization because of its high porosity and good adsorption effect, which can provide a habitat for microorganisms. The use of biochar immobilized microorganisms to treat different pollutants in wastewater is a promising treatment method. Compared with the other biological treatment technology, biochar immobilized microorganisms can improve microbial abundance, repeated utilization ratio, microbial metabolic capacity, etc. However, current research on this method is still in its infancy. Little attention has been paid to the interaction mechanisms between biochar and microorganisms, and many studies are only carried out in the laboratory. There are still problems such as difficult recovery after use and secondary pollution caused by residual pollutants after biochar adsorption, which need further clarification. To have comprehensive digestion and an in-depth understanding of biochar immobilized microorganisms technology in wastewater treatment, the wastewater treatment methods based on biochar are firstly summarized in this review. Then the mechanisms of immobilized microorganisms were explored, and the applications of biochar immobilized microorganisms in wastewater were systematically reviewed. Finally, suggestions and perspectives for future research and practical application are put forward.
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http://dx.doi.org/10.1016/j.scitotenv.2022.155563DOI Listing
April 2022

Proteomic Investigation Reveals Eukaryotic Translation Initiation Factor 5A Involvement in Porcine Reproductive and Respiratory Syndrome Virus Infection .

Front Vet Sci 2022 13;9:861137. Epub 2022 Apr 13.

Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, China.

Porcine reproductive and respiratory syndrome virus (PRRSV), one of the most serious animal pathogens in the world, has caused enormous global swine industry losses. An in-depth investigation of the PRRSV-host interaction would be beneficial for preventing and controlling PRRSV infections and transmission. In this study, we performed label-free quantitative proteomic assays to investigate proteome dynamics of porcine alveolar macrophages (PAMs) during infection with highly pathogenic PRRSV (HP-PRRSV) strain HN07-1. Analysis of the results led to identification of 269 significantly differentially expressed host cellular proteins, of which levels of proteins belonging to the eukaryotic translation initiation factor (eIF) family were found to be decreased in abundance in HP-PRRSV-infected PAMs. Furthermore, knockdown of eIF5A expression was demonstrated to markedly suppress HP-PRRSV propagation, as reflected by reduced progeny virus titers . These results highlight the importance of eIF5A in PRRSV infection, while also demonstrating that PAMs down-regulate eIF5A expression as a host cell antiviral strategy. Results of the current study deepen our understanding of PRRSV pathogenesis and provide novel insights to guide development of effective strategies to combat the virus.
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http://dx.doi.org/10.3389/fvets.2022.861137DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9043857PMC
April 2022

Electrochromic properties of pyrene conductive polymers modified by chemical polymerization.

RSC Adv 2021 Dec 9;11(62):39291-39305. Epub 2021 Dec 9.

School of Chemistry and Materials Science, Heilongjiang University Harbin 150080 PR China

Pyrene is composed of four benzene rings and has a unique planar melting ring structure. Pyrene is the smallest condensed polycyclic aromatic hydrocarbon, and its unique structural properties have been extensively studied. Pyrene has excellent properties such as thermal stability, high fluorescence quantum efficiency and high carrier mobility. This paper mainly used thiophene, EDOT and triphenylamine groups to enhance the pyrene based π-conjugated system and control the molecular accumulation of organic semiconductors, and improve their charge transport performances. Five kinds of polymer were synthesized and correspondingly characterized. The five kinds of pyrene conductive polymer had outstanding properties in terms of solubility, fluorescence intensity and thermal stability, good film-forming properties, stable electrochromic properties and high coloring efficiency. The coloration efficiency (CE) of PPYTP was as high as 277 cm C, and the switching response time was short. The coloring time of PPYEDOT was 1.3 s and the bleaching time was 3.2 s. The lower impedance will also provide the possibility of such polymers being incorporated into electrochromic devices in the future. In short, the synthesized new pyrene conductive polymers will have wide application prospects in the field of electrochromic materials.
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http://dx.doi.org/10.1039/d1ra07977hDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9044429PMC
December 2021

Prolonging the lifetime of a compact multi-wire-layered secondary winding in the Tesla transformer.

Rev Sci Instrum 2022 Apr;93(4):044703

Science and Technology on High Power Microwave Laboratory, Northwest Institute of Nuclear Technology, Xi'an, Shaanxi 710024, China.

A compact multi-wire-layered secondary winding for the Tesla transformer was proposed by Zhao et al. [Rev. Sci. Instrum. 88(5), 055112 (2017)]. The basic idea is to wind multiple layers of a metal wire around a polymeric base tube. However, the lifetime of this type of winding is only about 200 000 pulses, and thus it fails to meet the requirement of a lifetime of 1 × 10 pulses. In this study, two methods are developed to prolong the lifetime of this winding. One method involves replacing the original three-skin wire with a polytetrafluoroethylene (PTFE) wire. The results of small-scale experiments in different conditions show that the lifetime of the PTFE-covered copper wire is at least ten times longer than that of the three-skin wire. The other method involves improving the local structure of this winding. A strong mechanical stress is concentrated at the small end of the winding, and a highly intense electric field appears in this region, where both reduce the lifetime of the winding. Improving the local structure of the winding theoretically prolongs its lifetime by a factor of 4. Both methods were applied to the original secondary winding of a Tesla transformer and extended its theoretical lifetime by a factor of 40. The modified winding had a lifetime longer than 2 × 10 pulses without any traces of discharge. This is equivalent to a lifetime longer than that of the original winding by a factor of 10 and verifies the effectiveness of the proposed methods.
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http://dx.doi.org/10.1063/5.0083898DOI Listing
April 2022

Safety, tolerability, pharmacokinetics, and pharmacodynamics of oral JNJ-64794964, a TLR-7 agonist, in healthy adults.

Antivir Ther 2021 May 27;26(3-5):58-68. Epub 2021 Oct 27.

Janssen Research & Development LLC, Titusville, NJ, USA.

Background: This Phase I, two-part, first-in-human study assessed safety/tolerability and pharmacokinetics/pharmacodynamics of single-ascending doses (SAD) and multiple doses (MD) of the oral toll-like receptor-7 agonist, JNJ-64794964 (JNJ-4964) in healthy adults.

Methods: In the SAD phase, participants received JNJ-4964 0.2 ( = 6), 0.6 ( = 6), 1.25 ( = 8) or 1.8 mg ( = 6) or placebo ( = 2/dose cohort) in a fasted state. Food effect was evaluated for the 1.25 mg cohort following ≥6 weeks washout. In the MD phase, participants received JNJ-4964 1.25 mg ( = 6) or placebo ( = 2) weekly (fasted) for 4 weeks. Participants were followed-up for 4 weeks.

Results: No serious adverse events (AEs) occurred. 10/34 (SAD) and 5/8 (MD) participants reported mild-to-moderate (≤Grade 2), transient, reversible AEs possibly related to JNJ-4964. Five (SAD) participants had fever/flu-like AEs, coinciding with interferon-α serum levels ≥100 pg/mL and lymphopenia (<1 × 10/L), between 24-48 h after dosing and resolving approximately 96 h after dosing. One participant (MD) had an asymptomatic Grade 1 AE of retinal exudates (cotton wool spots) during follow-up, resolving 6 weeks after observation. JNJ-4964 exhibited dose-proportional pharmacokinetics, with rapid absorption (t 0.5-0.75 h) and distribution, and a long terminal half-life (150-591 h). Overall, no significant differences in JNJ-4964 pharmacokinetic parameters were observed in the fed versus fasted state. JNJ-4964 dose-dependently and transiently induced cytokines with potential anti-HBV activity, including interferon-α, IP-10, IL-1 RA, and/or MCP-1, and interferon-stimulated genes (ISG15, MX1, and OAS1) in serum.

Conclusions: In healthy adults, JNJ-4964 was generally well-tolerated, exhibited dose-proportional pharmacokinetics and induced cytokines/ISGs, with possible anti-HBV activity.
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http://dx.doi.org/10.1177/13596535211056581DOI Listing
May 2021

Urea-induced supramolecular self-assembly strategy to synthesize wrinkled porous carbon nitride nanosheets for highly-efficient visible-light photocatalytic degradation.

RSC Adv 2021 Jul 2;11(38):23459-23470. Epub 2021 Jul 2.

National Engineering Research Center of Industry Crystallization Technology, School of Chemical Engineering and Technology, Tianjin University Tianjin 300072 China

Graphitic carbon nitride (g-CN) has attracted immense interest as a promising photocatalyst. To facilitate its versatile applications in many fields, new low-cost strategies to synthesize outstanding g-CN need to be further developed. Although supramolecular preorganization has been considered as a promising candidate, the utilized supramolecules like melamine-cyanuric acid (MCA) are typically synthesized by expensive triazine derivatives. Herein, wrinkled porous g-CN nanosheets were successfully fabricated by hydrothermal-annealing of supramolecular intermediate MCA synthesized by the cheap precursors dicyandiamide and urea. During the formation of MCA, urea could act as a facile agent to react with dicyandiamide to form melamine and cyanuric acid firstly and then assemble into MCA through hydrogen bonds. In addition, urea could serve as a porogen and decompose to generate bubbles for conducive formation of micro-size MCA self-templates and thus wrinkled porous g-CN nanosheets could be obtained. The nanostructure and photocatalytic performance of g-CN were optimized by modulating microstructures and physicochemical properties of MCA, which could be conveniently controlled by urea addition and hydrothermal duration. The obtained wrinkled porous g-CN nanosheets exhibit highly-efficient visible-light photocatalytic degradation compared with traditional MCA-derived g-CN, which could remove 98.3% of the rhodamine B in 25 min. The superior photocatalytic activity is mainly attributed to the urea-induced larger specific surface area, better light harvesting ability, faster transfer and more advanced separation efficiency of the photogenerated electron-hole pairs. This research provides a new strategy for preparing high-performance porous g-CN from the self-assembled supramolecule MCA synthesized by low-cost precursors.
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http://dx.doi.org/10.1039/d1ra03524jDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9036594PMC
July 2021

Histone methyltransferase KMT2D cooperates with MEF2A to promote the stem-like properties of oral squamous cell carcinoma.

Cell Biosci 2022 Apr 27;12(1):49. Epub 2022 Apr 27.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory for Oral Biomedicine of Ministry of Education (KLOBM), School and Hospital of Stomatology, Wuhan University, Wuhan, 430089, China.

Background: Epigenetic reprogramming is involved in multiple steps of human cancer evolution and is mediated by a variety of chromatin-modifying enzymes. Specifically, the histone lysine methyltransferase KMT2D is among the most frequently mutated genes in oral squamous cell carcinoma (OSCC). However, the mechanisms by which KMT2D affects the development of OSCC remain unclear.

Results: In the present study, we found that the expression of KMT2D was elevated in OSCC compared to paracancerous specimens and was correlated with a more advanced tumor grade. More importantly, knockdown of KMT2D impaired their reconstitution in patient-derived organoids and decreased the expression of CD133 and β-catenin in OSCC cells. In in vitro and in vivo models, knockdown of KMT2D reduced the colony formation, migration and invasion abilities of OSCC cells and delayed tumor growth. Mechanistically, the dual-luciferase reporter and co-immunoprecipitation assays in two individual OSCC cell lines indicated that KMT2D may cooperate with MEF2A to promote the transcription activity of CTNNB1, thereby enhancing WNT signaling.

Conclusion: The upregulation of KMT2D contributes to stem-like properties in OSCC cells by sustaining the MEF2A-mediated transcriptional activity of CTNNB1.
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http://dx.doi.org/10.1186/s13578-022-00785-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9044881PMC
April 2022
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