Publications by authors named "Rui Jin"

290 Publications

Application of chelate GLDA for remediating Cd-contaminated farmlands using Tagetes patula L.

Environ Sci Pollut Res Int 2022 Aug 12. Epub 2022 Aug 12.

Anhui Province Key Lab of Farmland Ecological Conservation and Pollution Prevention, School of Resource and Environment, Anhui Agricultural University, Hefei, 230036, People's Republic of China.

In the present study, via a 180-day field trial, the indicators of soil total cadmium, DTPA-Cd, organic matter, and plant cadmium extraction were tested after the application of chelate tetrasodium glutamate diacetate (GLDA) to investigate the potential of GLDA combined with Tagetes patula L. to remediate cadmium-contaminated soil. To do so, five GLDA treatments (e.g., 0, 292.5, 585, 1170, and 2340 kg hm) were practiced. For each treatment, the total GLDA was divided into two applications with 15-day intervals (0.25, 0.47, and 0.61 mg·kg) under T. patula plantation. Compared with the control, our results showed that GLDA application significantly increased the biomass of aerial parts of T. patula by 21.9% (p < 0.05). Likewise, Cd content in aboveground and underground parts of T. patula increased by 94.7% and 60.5%, respectively, compared with the control (p < 0.05). GLDA application caused significant increases in Cd accumulations in cell soluble fraction and cell wall by 290% and 123%, respectively (p < 0.05); soil pH and DTPA-Cd content increased with the increase of total application of GLDA. Co-application of GLDA (2340 kg hm) and T. patula reduced the total soil Cd content by 12.87% compared with the soil background. Altogether, our findings conclude on the efficacy of GLDA application for the remediation of Cd-contaminated farmlands under T. patula cultivation.
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http://dx.doi.org/10.1007/s11356-022-22470-6DOI Listing
August 2022

The influence of different proton pump inhibitors and potassium-competitive acid blockers on indomethacin-induced small intestinal injury.

J Gastroenterol Hepatol 2022 Aug 8. Epub 2022 Aug 8.

Department of Gastroenterology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.

Background And Aim: The influence of gastric acid inhibitors (GAIs) on nonsteroidal anti-inflammatory drug (NSAID)-induced enteropathy is controversial. Herein, the influences of different GAIs on NSAID-induced intestinal injury and the underlying mechanisms are be clarified.

Method: Indomethacin (IND, 10 mg/kg/day) was administered to mice to induce small intestinal injury. Disease activity was examined macroscopically and histologically. The permeability of small intestine was evaluated by measuring plasma lipopolysaccharide (LPS) levels. 16S rDNA sequencing was performed to determine the composition of intestinal flora.

Results: Among the four GAIs, iprazole (IPZ) significantly attenuated IND-induced small intestinal injury and maintained the integrity of the mucosal barrier. Omeprazole (OPZ) and vonoprazan (VPZ) ameliorated ulceration without significant differences, while rabeprazole (RPZ) failed to protect against the injury. To explore the potential mechanism, we investigated changes in the gut microbiota mediated by GAIs. After five-days-administration, GAIs significantly altered the composition of the gut microbiota. The IND group had a significant decrease in alpha diversity compared with the control group, and this decrease was reversed by OPZ and IPZ treatment, respectively. After IPZ treatment, the community membership was more assembled in the control group than the IND group. Further, we found that Lactobacillus was significantly increased in the groups of OPZ, IPZ and VPZ, while Bacteroides were significantly increased in RPZ group.

Conclusion: Our results indicated that GAIs have different influences on the mucosal barrier, possibly by altering the composition of intestinal microbiota, and the impacts mediated by various GAIs in the IND-induced intestinal damage model seem different.
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http://dx.doi.org/10.1111/jgh.15973DOI Listing
August 2022

Artificial Intelligence-Assisted Bioinformatics, Microneedle, and Diabetic Wound Healing: A "New Deal" of an Old Drug.

ACS Appl Mater Interfaces 2022 Aug 1. Epub 2022 Aug 1.

Department of Plastic Surgery, Sir Run Run Shaw Hospital, Zhejiang University of Medicine, Hangzhou 310016, China.

Diabetic wounds severely influence life, facing grand challenges in clinical treatments. The demand for better treatment is growing dramatically. Diabetic wound healing is challenging because of inflammation, angiogenesis disruptions, and tissue remodeling. Based on sequencing results of diabetic patients' skins and artificial intelligence (AI)-assisted bioinformatics, we excavate a potential therapeutic agent Trichostatin A (TSA) and a potential target histone deacetylase 4 (HDAC4) for diabetic wound healing. The molecular docking simulation reveals the favorable interaction between TSA and HDAC4. Taking advantage of the microneedle (MN) minimally invasive way to pierce the skin barrier for drug administration, we develop a swelling modified MN-mediated patch loaded with TSA to reduce the probability of injection-caused iatrogenic secondary damage. The MN-mediated TSA patch has been demonstrated to reduce inflammation, promote tissue regeneration, and inhibit HDAC4, which provides superior results in diabetic wound healing. We envisage that our explored specific drug TSA and the related MN-mediated drug delivery system can provide an innovative approach for diabetic wound treatment with simple, effective, and safe features and find a broad spectrum of applications in related biomedical fields.
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http://dx.doi.org/10.1021/acsami.2c08994DOI Listing
August 2022

Reliability of Evidence to Guide Decision-Making in the Use of Acupuncture for Postpartum Depression.

Front Public Health 2022 14;10:942595. Epub 2022 Jul 14.

Nanchang Hongdu Hospital of Traditional Chinese Medicine, Nanchang, China.

Background: There is conflicting evidence on the effectiveness of acupuncture in the treatment of postpartum depression (PPD). This study aimed to assess previous systematic reviews/meta-analyses (SRs/MAs) on the effectiveness of acupuncture to treat PPD.

Method: SRs/MAs regarding the use of acupuncture for PPD were identified from the establishment of digital databases to November 2021. The Assessing the Methodological Quality of Systematic Reviews 2 (AMSTAR-2) was applied to evaluate the methodological quality of included SRs/MAs. The Grades of Recommendations, Assessment, Development and Evaluation (GRADE) was utilized to evaluate the evidence quality for outcomes of interest.

Results: Six studies that conducted quantitative syntheses were included. According to AMSTAR-2, the methodological quality of these SRs/MAs was critically low owing to limitations of items 2, 4, and 7. According to GRADE, no study included high-quality evidence and most studies included low-quality evidence.

Conclusions: Acupuncture m be beneficial for PPD, however, due to limitations of current evidence and inconsistent findings, further studies are needed to provide stronger evidence to draw definitive conclusions.
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http://dx.doi.org/10.3389/fpubh.2022.942595DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329701PMC
August 2022

Appendicular Skeletal Muscle Index and HbA1c Evaluate Liver Steatosis in Patients With Metabolic Associated Fatty Liver Disease.

Front Med (Lausanne) 2022 6;9:919502. Epub 2022 Jul 6.

Peking University People's Hospital, Peking University Hepatology Institute, Beijing Key Laboratory of Hepatitis C and Immunotherapy for Liver Diseases, Beijing International Cooperation Base for Science and Technology on NAFLD Diagnosis, Beijing, China.

Background And Aims: Liver steatosis, as the main feature of metabolic associated fatty liver disease (MAFLD), was associated with the progression of liver fibrosis and metabolic syndrome, which needed to be estimated accurately. In this study, we explored the significance of appendicular skeletal muscle index (ASMI) in evaluating liver steatosis of MAFLD patients.

Methods: Eight hundred and ninety-nine cases with MAFLD from 2017 to 2018 National Health and Nutrition Examination Surveys (NHANES) database were included. All the analyzed data were obtained from NHANES database. The association between ASMI and liver steatosis were evaluated using R and EmpowerStats.

Results: MAFLD individuals were randomly divided into a training ( = 450) and validation cohort ( = 449). In univariate analysis, HbA1c, arms fat, arms lean mass, legs lean mass, trunk lean mass, total fat, total lean mass and ASMI were significantly associated with liver steatosis ( < 0.05). Multivariate analysis showed that HbA1c (OR: 1.6732; 95% CI: 1.2753-2.1929, = 0.0002) and ASMI (OR: 1.6723; 95% CI: 1.1760-2.5204, = 0.0052) were independently associated with severe liver steatosis. ASMI accurately evaluated severe liver steatosis with an AUROC of 0.73 and 0.81 in training and validation cohort, respectively. Compared with ASMI only, ASMI combined with HbA1c improved the AUROC to 0.85 and 0.88. Furthermore, the AUROC of our model was superior to FLI in the evaluation of liver steatosis.

Conclusion: ASMI combined with HbA1c has good evaluation value for liver steatosis in MAFLD patients, which might be beneficial for the management of MAFLD clinically.
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http://dx.doi.org/10.3389/fmed.2022.919502DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9298827PMC
July 2022

The Lateral Orbital Thickening and the Lateral Brow Thickening: A New Choice for the Lower Eyelid Suspension.

J Craniofac Surg 2022 Jul 22. Epub 2022 Jul 22.

Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai.

This study aimed to evaluate the effectiveness and safety of a double-suspension technique using the lateral orbital thickening (LOT) and the lateral brow thickening (LBT) in patients with lower eyelid defects. A total of 5 cadavers (4 males and 1 female) ranging in age from 50 to 78 years was subjected to observe the distribution of the orbicularis retaining ligament (ORL) and its characteristics. The ORL is a closed-loop structure that forms the LBT outside the supraorbital rim and forms the LOT in the lateral canthal region. A total of 17 patients (12 females and 5 males) with an average age of 12.3 years who underwent the ORL-assisted lower eyelid reconstruction were included in this retrospective study. After the ORL was completely released on the infraorbital rim for reducing down traction, the subocularis oculi fat and the orbicularis oculi were fixed to the LBT and the LOT for increasing the upward traction. The lower eyelid defects in 17 patients were reduced in different degrees. The defects were closed directly in 3 patients and were resurfaced by skin graft or flap in the other 14 patients. Physicians and patients were all satisfied with the final cosmetic results. Thus, the ORL is a rigid and restrictive internal connected structure, and the ORL-assisted technique decreases the defect size and reconstructs vertical force balance. The LOT and the LBT are strong and safe enough as new fixation choices for lower eyelid suspension.
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http://dx.doi.org/10.1097/SCS.0000000000008718DOI Listing
July 2022

Deep learning to diagnose Hashimoto's thyroiditis from sonographic images.

Nat Commun 2022 06 29;13(1):3759. Epub 2022 Jun 29.

Tianjin Cancer Institute, Tianjin's Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, National Clinical Research Center for Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin Medical University, Tianjin, China.

Hashimoto's thyroiditis (HT) is the main cause of hypothyroidism. We develop a deep learning model called HTNet for diagnosis of HT by training on 106,513 thyroid ultrasound images from 17,934 patients and test its performance on 5051 patients from 2 datasets of static images and 1 dataset of video data. HTNet achieves an area under the receiver operating curve (AUC) of 0.905 (95% CI: 0.894 to 0.915), 0.888 (0.836-0.939) and 0.895 (0.862-0.927). HTNet exceeds radiologists' performance on accuracy (83.2% versus 79.8%; binomial test, p < 0.001) and sensitivity (82.6% versus 68.1%; p < 0.001). By integrating serologic markers with imaging data, the performance of HTNet was significantly and marginally improved on the video (AUC, 0.949 versus 0.888; DeLong's test, p = 0.004) and static-image (AUC, 0.914 versus 0.901; p = 0.08) testing sets, respectively. HTNet may be helpful as a tool for the management of HT.
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http://dx.doi.org/10.1038/s41467-022-31449-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9243092PMC
June 2022

Carbon dioxide effects on daytime sleepiness and EEG signal: A combinational approach using classical frequentist and Bayesian analyses.

Indoor Air 2022 Jun;32(6):e13055

Division of Biomedical Engineering for Health & Welfare, Tohoku University Graduate School of Biomedical Engineering, Sendai, Miyagi, Japan.

Environmental carbon dioxide (CO ) could affect various mental and physiological activities in humans, but its effect on daytime sleepiness is still controversial. In a randomized and counterbalanced crossover study with twelve healthy volunteers, we applied a combinational approach using classical frequentist and Bayesian statistics to analyze the CO exposure effect on daytime sleepiness and electroencephalogram (EEG) signals. Subjective sleepiness was measured by the Japanese Karolinska Sleepiness Scale (KSS-J) by recording EEG during CO exposure at different concentrations: Normal (C), 4000 ppm (Moderately High: MH), and 40 000 ppm (high: H). The daytime sleepiness was significantly affected by the exposure time but not the CO condition in the classical statistics. On the other hand, the Bayesian paired t-test revealed that the CO exposure at the MH condition might induce daytime sleepiness at the 40-min point compared with the C condition. By contrast, EEG was significantly affected by a short exposure to the H condition but not exposure time. The Bayesian analysis of EEG was primarily consistent with results by the classical statistics but showed different credible levels in the Bayes' factor. Our result suggested that the EEG may not be suitable to detect objective sleepiness induced by CO exposure because the EEG signal was highly sensitive to environmental CO concentration. Our study would be helpful for researchers to revisit whether EEG is applicable as a judgment indicator of objective sleepiness.
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http://dx.doi.org/10.1111/ina.13055DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9327715PMC
June 2022

Reconstruction of Facial Multiunit Defects Using Expanded Scalp Flap with Laser Depilation in a Group of Predominantly Pediatric Patients.

Facial Plast Surg Aesthet Med 2022 Jun 10. Epub 2022 Jun 10.

Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

The cervical or thoracic region is currently the main donor site for repair of facial multiunit defects (FMUDs); however, its extensive clinical utilization encompasses the need for flap prefabrication and microsurgical techniques. To illuminate the feasibility of treating FMUD with expanded scalp flaps and laser depilation. From 2016 to 2020, 17 patients with facial giant congenital melanocytic nevi (GCMN) underwent scalp expansion for FMUD reconstruction. A three-stage surgical treatment plan was attempted and laser depilation was scheduled during the expansion period and postrepair surgeries. All the flaps survived successfully and were able to cover the entire facial defect. The average three-stage treatment time was 4.5 ± 1.3 months. The defect sites included the forehead, nose, cheek, temporal, and eye areas. The maximum defect area was 9 × 30 cm. The average number of laser treatments was 4.6 ± 2.9 times. The average treatment period was 26.8 months. Sixteen patients (94.1%) reported satisfactory outcome. Expanded scalp flap combined with laser depilation is feasible for FMUD repair and warrants further investigation. Clinical Trail Registration Information: Translational Medicine Ethics Review Committee, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine (SH9H-2021-T144-3).
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http://dx.doi.org/10.1089/fpsam.2021.0279DOI Listing
June 2022

[Design and Verification of Human Metabolic Measurement System Based on STM32].

Zhongguo Yi Liao Qi Xie Za Zhi 2022 May;46(3):273-277

Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei, 230031.

A high-precision human metabolic measurement system is designed. The system uses STM32F103 as the main control chip to acquire oxygen, carbon dioxide and flow signals to calculate four quantitative indicators: oxygen consumption(), carbon dioxide production(), respiratory entropy() and resting energy metabolism(), and finally uses an upper computer to display the calculation results.In this paper, the signal acquisition circuit design was carried out for the oxygen sensor, carbon dioxide sensor and flow sensor, and the validity of the device was verified with the American machine MGCDiagnositcs using Bland-Altman analysis method, and the results showed that the four parameters of ,, and of both devices fell in the agreement interval of more than 95%. The device thus provides accurate metabolic measurements and offers an effective tool for the field of general health and clinical nutrition support in China.
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http://dx.doi.org/10.3969/j.issn.1671-7104.2022.03.008DOI Listing
May 2022

Lactate Dehydrogenase B Is Required for Pancreatic Cancer Cell Immortalization Through Activation of Telomerase Activity.

Front Oncol 2022 20;12:821620. Epub 2022 May 20.

Department of Cell Engineering, Beijing Institute of Biotechnology, Beijing, China.

Telomerase activity is elevated in most cancer cells and is required for telomere length maintenance and immortalization of cancer cells. Glucose metabolic reprogramming is a hallmark of cancer and accompanied with increased expression of key metabolic enzymes. Whether these enzymes influence telomerase activity and cell immortalization remains unclear. In the current study, we screened metabolic enzymes using telomerase activity assay and identified lactate dehydrogenase B (LDHB) as a regulator of telomerase activity. Sodium lactate and sodium pyruvate did not influence telomerase activity, indicating LDHB regulates telomerase activity independent of its metabolism regulating function. Further studies revealed that LDHB directly interacted with TERT and regulated the interaction between TERT and TERC. Additionally, long-term knockdown of LDHB inhibited cancer cell growth and induced cell senescence and . Higher LDHB expression was detected in pancreatic cancer tissues compared with that in adjacent normal tissues and expression of LDHB correlated negatively with prognosis. Thus, we identified LDHB as the first glucose metabolic enzyme contributing to telomerase activity and pancreatic cancer cell immortalization.
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http://dx.doi.org/10.3389/fonc.2022.821620DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9163669PMC
May 2022

Satellite-Enabled Internet of Remote Things Network Transmits Field Data from the Most Remote Areas of the Tibetan Plateau.

Sensors (Basel) 2022 May 13;22(10). Epub 2022 May 13.

National Tibetan Plateau Data Center, State Key Laboratory of Tibetan Plateau Earth System, Resources and Environment (TPESRE), Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, China.

In this article, we employed a satellite-enabled Internet of Remote Things (IoRT) network as a promising solution to retrieve data in the most remote areas of interest, where public networks are absent. This article presents a system network based on the satellite-enabled IoRT, a new paradigm that defines a network where each environmental monitoring device can autonomously establish a network with a remote data center. The Xingyun satellite constellation was employed for data retrieval on the Tibetan Plateau (TP). The monitoring system was mainly composed of a ground Internet of Things (IoT) terminal that was built with satellite transceivers, environmental monitoring devices, and system software. We deployed five of these newly developed terminals in harsh areas to monitor environmental variables, and accordingly, air temperature and relative humidity, precipitation, snow depth, land surface temperature, tree stemflow rate, and photosynthetically active radiation were retrieved with the satellite-enabled IoRT network. Field experiments were conducted to evaluate the performance of the proposed system network, and the results indicated that the average time delay with and without the packet creation mode reached 32 and 32.7 s, respectively, and the average packet loss rate with and without the packet creation mode reached 5.63% and 4.48%, respectively. The successful implementation of the satellite-enabled IoRT network for the rapid retrieval of monitoring data in remote glacier, forestland, and canyon areas at very high altitudes on the TP provides an entirely new and revolutionary data retrieval means for backhauling data from remote areas of interest.
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http://dx.doi.org/10.3390/s22103713DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9147722PMC
May 2022

Mid-upper arm circumference is associated with liver steatosis and fibrosis in patients with metabolic-associated fatty liver disease: A population based observational study.

Hepatol Commun 2022 May 13. Epub 2022 May 13.

Peking University Hepatology Institute, Beijing Key Laboratory of Hepatitis C and Immunotherapy for Liver Diseases, Beijing International Cooperation Base for Science and Technology on NAFLD Diagnosis, Peking University People's Hospital, Beijing, China.

Metabolic-associated fatty liver disease (MAFLD) is a series of liver diseases based on liver steatosis and metabolic disorders. Steatosis, as the core factor in MAFLD diagnosis, and fibrosis, as the major determinant of adverse outcomes of MAFLD, need to be assessed simply and accurately. In this study, we explored the significance of mid-upper arm circumference (MUAC) in evaluating liver steatosis and fibrosis in patients with MAFLD. We included 2397 cases with MAFLD from the 2017-2018 National Health and Nutrition Examination Surveys (NHANES) database. Liver steatosis and fibrosis were measured by vibration controlled transient elastography. Anthropometric parameters and demographic and serological data were obtained from the NHANES database. The association between MUAC and liver steatosis and fibrosis were evaluated by a multivariable linear regression model, a weighted generalized additive model, and smooth curve fitting using R. MUAC was positively associated with liver steatosis in every multivariate linear regression model (model 1: β = 3.3513; 95% confidence interval [CI], 2.7722-3.9304; model 2: β = 3.8492; 95% CI, 3.2441-4.4542; model 3: β = 2.4987; 95% CI, 1.8371-3.1604), and this positive association was consistent in both men and women and among different race groups (Mexican American, other Hispanic, non-Hispanic White, Black, Asian, and other race). On the other hand, MUAC was positively associated with liver fibrosis in every multivariate linear regression model, and this positive association also was consistent in both men and women and among non-Hispanic White and Black populations. Increased MUAC was positively associated with liver steatosis and fibrosis in patients with MAFLD. This was particularly true for MUAC ≥ 42.0 cm. MUAC might be a simple and convenient evaluation tool for MAFLD.
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http://dx.doi.org/10.1002/hep4.1990DOI Listing
May 2022

Water and Meadow Views Both Afford Perceived but Not Performance-Based Attention Restoration: Results From Two Experimental Studies.

Front Psychol 2022 25;13:809629. Epub 2022 Apr 25.

School of Ecosystem and Forest Sciences, The University of Melbourne, Parkville, VIC, Australia.

Attention Restoration Theory proposes that exposure to natural environments helps to restore attention. For sustained attention-the ongoing application of focus to a task, the effect appears to be modest, and the underlying mechanisms of attention restoration remain unclear. Exposure to nature may improve attention performance through many means: modulation of alertness and one's connection to nature were investigated here, in two separate studies. In both studies, participants performed the Sustained Attention to Response Task (SART) before and immediately after viewing a meadow, ocean, or urban image for 40 s, and then completed the Perceived Restorativeness Scale. In Study 1 ( = 68), an eye-tracker recorded the participants' tonic pupil diameter during the SARTs, providing a measure of alertness. In Study 2 ( = 186), the effects of connectedness to nature on SART performance and perceived restoration were studied. In both studies, the image viewed was not associated with participants' sustained attention performance; both nature images were perceived as equally restorative, and more restorative than the urban image. The image viewed was not associated with changes in alertness. Connectedness to nature was not associated with sustained attention performance, but it did moderate the relation between viewing the natural images and perceived restorativeness; participants reporting a higher connection to nature also reported feeling more restored after viewing the nature, but not the urban, images. Dissociation was found between the physiological and behavioral measures and the perceived restorativeness of the images. The results suggest that restoration associated with nature exposure is not associated with modulation of alertness but is associated with connectedness with nature.
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http://dx.doi.org/10.3389/fpsyg.2022.809629DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9084315PMC
April 2022

Curvature dependence of BAR protein membrane association and dissociation kinetics.

Sci Rep 2022 05 10;12(1):7676. Epub 2022 May 10.

Department of Chemistry, University of Pennsylvania, Philadelphia, PA, USA.

BAR (Bin/Amphiphysin/Rvs) domain containing proteins function as lipid bilayer benders and curvature sensors, and they contribute to membrane shaping involved in cell signaling and metabolism. The mechanism for their membrane shape sensing has been investigated by both equilibrium binding and kinetic studies. In prior research, stopped-flow spectroscopy has been used to deduce a positive dependence on membrane curvature for the binding rate constant, k, of a BAR protein called endophilin. However, the impact of bulk diffusion of endophilin, on the kinetic binding parameters has not been thoroughly considered. Employing similar methods, and using lipid vesicles of multiple sizes, we obtained a linear dependence of k on vesicle curvature. However, we found that the observed relation can be explained without considering the local curvature sensing ability of endophilin in the membrane association process. In contrast, the diffusion-independent unbinding rate constant (k) obtained from stopped-flow measurements shows a negative dependence on membrane curvature, which is controlled/mediated by endophilin-membrane interactions. This latter dependency, in addition to protein-protein interactions on the membrane, explains the selective binding of BAR proteins to highly curved membranes in equilibrium binding experiments.
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http://dx.doi.org/10.1038/s41598-022-11221-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9091223PMC
May 2022

Management of Complications in 257 Cases of Breast Augmentation with Polyacrylamide Hydrogel, using Two Different Strategies: A Retrospective Study.

Aesthetic Plast Surg 2022 Apr 20. Epub 2022 Apr 20.

Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, 639 ZhiZao Ju Road, Shanghai, 200011, People's Republic of China.

Background: Polyacrylamide hydrogel (PAAG) has been used globally for breast augmentation, leading to long-term clinical complications. However, whether the infiltrated fibrotic capsule should be removed with PAAG to alleviate the complications remains unclear. This study aimed to ascertain different causes of complications and proper management strategies for PAAG removal in augmented breasts.

Methods: From July 2015 to December 2019, patients who underwent breast augmentation with PAAG and in whom surgical intervention was undertaken for PAAG-associated adverse events at Shanghai Ninth People's Hospital were retrospectively reviewed. Patients were categorized into two groups according to whether the fibrotic capsule was removed (RFC) or not (NRFC). Aesthetic outcomes, PAAG residues, and adverse events were evaluated post-operatively to assess whether important issues pertaining to these arose following fibrotic capsule removal. Tissue histology and PAAG degradation analysis were implemented to investigate immune response, degradability, and toxicity of PAAG.

Results: Altogether, 257 patients (88 RFC and 169 NRFC patients) were enrolled. 73.4% and 79.5% of the RFC and NRFC groups showed fairly good outcomes, with no significant difference, respectively. (X= 0.0804, p = 0.79) Significant differences were found between two surgical techniques upon patient satisfaction, respectively. (X= 3.529; p = 0.0301). Predictor of poor outcomes identified scar (OR, 4.555, p = 0.0019) and PAAG residue (OR, 5.379, p = 0.0003). Predictor of patient satisfaction identified post-operative outcomes (OR, 3.797; 95% CI, 1.860-8.923; p = 0.0002) and surgical technique (NRFC) (OR, 2.519; 95% CI, 1.449-4.434; p = 0.0008).

Conclusions: Both treatment strategies showed good results in our study. Removal of the fibrotic capsule from infiltration of PAAG largely depends on the individual psychological condition, aesthetic expectations, complications, and magnetic resonance imaging results. While PAAG does not degrade in the host's body over time, it may elicit immune reactions and chronic inflammation in the long term.

Level Of Evidence Iv: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine Ratings, please refer to Table of Contents or online Instructions to Authors www.springer.com/00266.
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http://dx.doi.org/10.1007/s00266-022-02876-wDOI Listing
April 2022

Anchored Suture Technique for the Reconstruction of Paranasal Skin Defects Secondary to Melanocytic Nevus Excision.

J Craniofac Surg 2021 Oct 26. Epub 2021 Oct 26.

Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011.

To describe a modified anchored suture technique combined with varied flaps for the repair of paranasal skin defect secondary to melanocytic nevus excision. The feasibility and effectiveness of the technique were discussed. A total of 26 patients (10 male and 16 female) with an average age of 11.1 years were included in this retrospective study. All patients underwent the anchored suture technique. The subcutaneous tissue of the free margin of the cheek flap was sutured to the deep pyriform ligament. The local flaps were designed according to the size and shape of the defect. The diameter of the nevi ranged from 2.8 to 7.5 cm, with most being 3 to 5 cm (50%). Among the 26 patients, 17 patients underwent the anchored suture technique and nonadvancement flap, whereas the other 9 patients underwent the anchored suture technology and advancement flap with auxiliary incisions. Twenty-five patients had a symmetric nasal alar and unapparent scar and were satisfied with postoperative aesthetic outcomes. Thus, the anchored suture method combined with different flaps to repair paranasal defect is an effective and affordable technique to reconstruct paranasal tissue connections.
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http://dx.doi.org/10.1097/SCS.0000000000008320DOI Listing
October 2021

The CD146-HIF-1α axis regulates epithelial cell migration and alveolar maturation in a mouse model of bronchopulmonary dysplasia.

Lab Invest 2022 08 19;102(8):794-804. Epub 2022 Mar 19.

Department of Neonatal Medical Center, Children's Hospital of Nanjing Medical University, Nanjing, China.

Bronchopulmonary dysplasia (BPD) is the most common challenge in preterm neonates. Retardation of alveolar development characterizes the pulmonary pathology in BPD. In the present study, we explored the roles of the CD146-HIF-1α axis in BPD. We demonstrated that the levels of reactive oxygen species (ROS) and soluble CD146 (sCD1146) were increased in the peripheral blood of preterm neonates with BPD. In alveolar epithelial cells, hyperoxia promoted the expression of HIF-1α and CD146, which reinforced each other. In a mouse model of BPD, by exposing pups to 65% hyperoxia, HIF-1α and CD146 were increased in the pulmonary tissues. Mechanistically, CD146 hindered the migration of alveolar epithelial cells; in contrast, movement was significantly enhanced in CD146-knockout alveolar epithelial cells. As expected, CD146-knockout ameliorated alveolarization and improved BPD disease severity. Taken together, our findings imply that the CD146-HIF-1α axis contributes to alveolarization and that CD146 may be a novel candidate in BPD therapy.
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http://dx.doi.org/10.1038/s41374-022-00773-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309096PMC
August 2022

Combination therapy of ursodeoxycholic acid and glucocorticoid and (or) immunosuppressant in patients with primary biliary cholangitis: A meta-analysis.

Medicine (Baltimore) 2022 Mar;101(9):e28987

Peking University Hepatology Institute, Peking University People's Hospital, Beijing, China.

Background/objectives: Primary biliary cholangitis (PBC) is a chronic inflammatory autoimmune cholestasis liver disease. There were many studies comparing a combination of glucocorticoids and/or immunosuppressants to a single UDCA therapy in PBC patients, while the literature demonstrated divergent finds. To evaluate the effectiveness of ursodeoxycholic acid (UDCA) combined with glucocorticoids and (or) immunosuppressants on biochemistry, immunology, histology, clinical symptoms, and adverse reactions of PBC from the perspective of evidence-based medicine.

Materials And Methods: PubMed, web of science, the Cochrane Library, EMBASE databases were searched to collect clinical randomized trials and self-control studies of UDCA combined with glucocorticoids and (or) immunosuppressants and UDCA monotherapy in the treatment of PBC. The retrieval time is from the establishment of the database to August 2020. Two reviewers independently screened literature, extracted data and evaluated the bias of included studies. Revman 5.3 software was used for meta-analysis.

Results: Six studies including 201 patients were included. The meta-analysis found that the combination therapy can improve some biochemical indexes, immunological indexes, and clinical symptoms of patients with PBC. However, combination therapy has no significant improvement in other biochemical indicators which respond to liver and bile duct damage, such as ALT, GGT, and ALB. Besides, the improvement of liver histology is limited, and the incidence of adverse events is higher.

Conclusion: Overall, the combination therapy showed no improvement in key biochemical parameters and limited improvement in liver pathology. Besides, the side effects were more serious. Therefore, in the current treatment regimen, it is not recommended for PBC patients.
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http://dx.doi.org/10.1097/MD.0000000000028987DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8896518PMC
March 2022

Insights into the evolution of the ISG15 and UBA7 system.

Genomics 2022 03 5;114(2):110302. Epub 2022 Feb 5.

The State Key Laboratory Breeding Base of Bioresources and Eco-environments, Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Laboratory of Molecular Developmental Biology, School of Life Sciences, Southwest University, Beibei 400715, Chongqing, China. Electronic address:

The genetic origins of novelty are of central interest in evolutionary biology. ISG15 and UBA7 are present only in vertebrates. The emergence and evolution of them are not clear. Phylogenetic comparisons revealed that UBA7 descends from gene duplication, and ISG15 and UBA7 arose from UBB/UBC and UBA1, respectively. Uba7 exhibits ubiquitin-activation activity in fish but not tetrapods, suggesting that the relationship of ISG15/Uba7 was promiscuous in origin but was later coopted toward higher specificity. Zebrafish Uba7 is capable of activating the ubiquitin cascade in vitro and in vivo, and it displays distinct specificity preference toward substrates and E2 enzymes compared to zebrafish Uba1. These results together provide a framework for understanding the origin and diversification of ISG15/Uba7 and may serve as a paradigmatic example in which an originally minor functionality in an old gene is made into a new high-specificity protein through random mutations and natural selection.
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http://dx.doi.org/10.1016/j.ygeno.2022.110302DOI Listing
March 2022

Therapeutic efficacy and mechanism of CD73-TGFβ dual-blockade in a mouse model of triple-negative breast cancer.

Acta Pharmacol Sin 2022 Jan 26. Epub 2022 Jan 26.

Department of Pharmacology, Fudan University School of Pharmacy, Shanghai, 201203, China.

Although chemotherapy and recently approved immunotherapies have improved treatment of triple-negative breast cancer (TNBC), the clinical outcome for this deadly disease remains unsatisfactory. We found that both cluster of differentiation 73 (CD73) and transforming growth factor (TGF)β were elevated in TNBC and correlated with the epithelial-mesenchymal transition (EMT), fibrotic stroma, an immune-tolerant tumor environment, and poor prognosis. To explore the efficacy of CD73-TGFβ dual-blockade, we generated a bifunctional anti-CD73-TGFβ construct consisting of the CD73 antibody MEDI9447 fused with the TGFβRII extracellular-domain (termed MEDI-TGFβR). MEDI-TGFβR retained full and simultaneous blocking efficiency for CD73 and TGFβ. Compared with MEDI9447 activity alone, MEDI-TGFβR demonstrated superior inhibitory activity against CD73-dependent cell migration and the EMT in CD73-high TNBC cells and effectively reduced lung metastasis in a syngeneic mouse model of TNBC. Mechanistically, the CD73-TGFβ dual-blockade reverted the EMT and stromal fibrosis and induced tumor cell death, which was accompanied by the accumulation of M1-macrophages and production of tumor necrosis factor α (TNFα). The CD73-TGFβ dual-blockade promoted a multifaceted inflammatory tumor microenvironment, as shown by the diminished levels of myeloid-derived suppressor cells (MDSCs) and M2-macrophages, and substantially increased levels of activated dendritic cells, cytotoxic T cells, and B cells. Collectively, our results have highlighted a novel strategy for TNBC treatment.
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http://dx.doi.org/10.1038/s41401-021-00840-zDOI Listing
January 2022

Tofacitinib for extraintestinal manifestations of inflammatory bowel disease: A literature review.

Int Immunopharmacol 2022 Apr 18;105:108517. Epub 2022 Jan 18.

Department of Gastroenterology, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Science, Beijing, China. Electronic address:

Background And Aims: Extraintestinal manifestations (EIMs) are commonly seen in patients with inflammatory bowel disease (IBD); management of EIMs is difficult and increases the primary disease burden. Recently, tofacitinib (TOF) was reported to be a promising option for treatment of EIMs. We aimed to review published articles and report experience to date.

Methods: The PubMed, Cochrane Library, and Web of Science databases were searched to identify eligible studies. The inclusion criteria were as follows: confirmed diagnosis of IBD; definitive EIMs; treatment with TOF; human study and published in English. The Newcastle-Ottawa Scale score and Cochrane Collaboration's tool for assessing risk of bias were used to determine the quality of the selected studies.

Results: Twenty-three studies met the inclusion criteria and were included. For nonrandomized studies, 16 were low quality, 5 were moderate quality, and 1 was high quality. For the one randomized controlled trial, the overall bias risk was low. The most concerning EIMs were dermatological manifestations, rheumatologic manifestations, and others, such as primary sclerosing cholangitis, autoimmune hepatitis, uveitis, and Takayasu arteritis. After administering doses of 5-20 mg/d TOF, the included studies reported varying degrees of clinical remission for both the primary disease and EIMs, except for musculoskeletal EIMs.

Conclusion: TOF might benefit EIMs in IBD, especially ulcerative colitis, and elevated dosages and longer treatment times may increase its effectiveness. Manifestation-specific results and large prospective studies are highly warranted.
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http://dx.doi.org/10.1016/j.intimp.2022.108517DOI Listing
April 2022

Sub-brow skin excision Combined with retro-orbicularis fat resection: A Technique for upper eyelid bulkiness and laxity correction.

J Plast Reconstr Aesthet Surg 2022 04 27;75(4):1431-1437. Epub 2021 Nov 27.

Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China. Electronic address:

Background: Many people are characterized by a swollen appearance and loose skin of upper eyelids, especially Asians. Retro-orbicularis oculus fat (ROOF) lying in the lateral canthus area is a critical factor of the bulkiness formation. Although several approaches have been reported, most of them intend to remove orbicularis muscle and preaponeurotic fat through a double-eyelid incision, leaving ROOF untreated. Consequently, an unnatural eyelid fold is formed and the lateral supraorbital region remains bloated .

Methods: Sixty-seven patients underwent sub-brow skin excision combined with ROOF resection. The key point was to excise the loose skin and thick ROOF through an incision along with the lower 1/3 of the eyebrow. Surgical outcomes were evaluated 6 months postoperatively by the heights of the designed line from the palpebral margin to the pupil center (HPPC), medial cornea (HPMC), and lateral canthus (HPLC). The pinch test, photographs, and complications were also recorded.

Results: The preoperative average HPPC, HPMC, and HPLC were 9.68 ± 1.73, 7.68 ± 1.24, and 6.82 ± 1.12 mm, while the postoperative average measurements were 7.25 ± 0.51, 5.99 ± 0.54, and 5.54 ± 0.61 mm, respectively. The result of the pinch test was improved and postoperative scarring was inconspicuous. Two patients had transient numbness in the eyebrow region that subsided within seven days. Two patients had slight asymmetry of eyebrow position but did not need a second operation.

Conclusions: Sub-brow skin excision combined with ROOF resection is an effective technique for improving upper eyelid bulkiness and skin excess with low revision rates.
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http://dx.doi.org/10.1016/j.bjps.2021.11.059DOI Listing
April 2022

The safety and efficacy of gene therapy treatment for monogenic retinal and optic nerve diseases: A systematic review.

Genet Med 2022 03 30;24(3):521-534. Epub 2021 Nov 30.

Department of Optometry and Vision Sciences, Melbourne School of Health Sciences, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Parkville, Victoria, Australia; Department of Surgery (Ophthalmology), Melbourne Medical School, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Parkville, Victoria, Australia; Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, Melbourne, Victoria, Australia.

Purpose: This study aimed to systematically review and summarize gene therapy treatment for monogenic retinal and optic nerve diseases.

Methods: This review was prospectively registered (CRD42021229812). A comprehensive literature search was performed in Ovid MEDLINE, Ovid Embase, Cochrane Central, and clinical trial registries (February 2021). Clinical studies describing DNA-based gene therapy treatments for monogenic posterior ocular diseases were eligible for inclusion. Risk of bias evaluation was performed. Data synthesis was undertaken applying Synthesis Without Meta-analysis guidelines.

Results: This study identified 47 full-text publications, 50 conference abstracts, and 54 clinical trial registry entries describing DNA-based ocular gene therapy treatments for 16 different genetic variants. Study summaries and visual representations of safety and efficacy outcomes are presented for 20 unique full-text publications in RPE65-mediated retinal dystrophies, choroideremia, Leber hereditary optic neuropathy, rod-cone dystrophy, achromatopsia, and X-linked retinoschisis. The most common adverse events were related to lid/ocular surface/cornea abnormalities in subretinal gene therapy trials and anterior uveitis in intravitreal gene therapy trials.

Conclusion: There is a high degree of variability in ocular monogenic gene therapy trials with respect to study design, statistical methodology, and reporting of safety and efficacy outcomes. This review improves the accessibility and transparency in interpreting gene therapy trials to date.
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http://dx.doi.org/10.1016/j.gim.2021.10.013DOI Listing
March 2022

Folic Acid-Modified miR-491-5p-Loaded ZIF-8 Nanoparticles Inhibit Castration-Resistant Prostate Cancer by Regulating the Expression of EPHX1.

Front Bioeng Biotechnol 2021 22;9:706536. Epub 2021 Nov 22.

Department of Urology, The First Affiliated Hospital of Soochow University, Suzhou, China.

Epoxide hydrolase 1 (EPHX1) has been reported to be related to the development of several tumors. However, the regulation of castration-resistant prostate cancer (CRPC) development by EPHX1 has not been reported. We used proteomic technology and found that the EPHX1 protein was highly expressed in CRPC tissues and the CRPC cell line C4-2. We performed screening and found that EPHX1 is a direct target of miR-491-5p. High miR-491-5p expression significantly reduced the EPHX1 level in C4-2 cells and inhibited C4-2 cell proliferation and migration. Zeolite imidazolate framework-8 (ZIF-8) has good thermal stability, a simple synthesis method, tumor site stability, and specific acid responsiveness. We synthesized ZIF-8 nanodrug vectors to deliver miR-491-5p into C4-2 cells. After loading miR-491-5p into ZIF-8, we modified the ZIF-8 surface with folic acid (FA) as the target group ([email protected]). Our synthesized nanodrug carrier showed less cytotoxicity to C4-2 cells even at 200 μg/ml. Modified FA could increase the efficiency of nanomaterial entry into C4-2 cells. [email protected]@ZIF-8 could stably release miR-491-5p for a long period in both phosphate-buffered saline (pH 7.4) and acetate buffer (pH 4.8), and miR-491-5p was released faster at the beginning of the experiment in acetate buffer (pH 4.8). [email protected]@ZIF-8 significantly reduced C4-2 cell proliferation and migration, and [email protected]@ZIF-8 had a better effect than miR-491-5p alone. , [email protected]@ZIF-8 significantly inhibited CRPC growth in nude mice. Overall, we verified that miR-491-4p regulated CRPC development by targeting EPHX1. The drug nanocarrier [email protected]@ZIF-8 not only significantly reduced C4-2 CRPC cell proliferation and migration but also significantly inhibited CRPC growth. Our research provides a theoretical basis for treatment and treatment strategies for CRPC.
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http://dx.doi.org/10.3389/fbioe.2021.706536DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8645958PMC
November 2021

The ultrafast, high-pitch turbo FLASH mode of third-generation dual-source CT: Effect of different pitch and corresponding SFOV on image quality in a phantom study.

J Appl Clin Med Phys 2021 Dec 9;22(12):158-167. Epub 2021 Nov 9.

Department of Radiology, the First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Purpose: To investigate the effect of different pitches and corresponding scan fields of view (SFOVs) on the image quality in the ultrafast, high-pitch turbo FLASH mode of the third-generation dual-source CT using an anthropomorphic phantom.

Methods: The phantom was scanned using the ultrafast, high-pitch turbo FLASH protocols of the third-generation dual-source CT with the different pitches and corresponding SFOVs (pitches: 1.55 to 3.2 with increments of 0.1, SFOVs: 50 cm to 35.4 cm). The objective parameters such as the CT number, image noises, signal-to-noise ratio (SNR), contrast-to-noise ratio (CNR), and artifacts index (AI), and image features from the head, chest, and abdomen were compared between the CT images with a pitch of 1.55 and SFOV of Ø 50 cm and a pitch of 3.2 and SFOV of Ø 35.4 cm. Then, the 18 series of CT images of the head, chest, and abdomen were evaluated by three radiologists independently.

Results: The differences in the CT numbers were not statically significant between the CT images with a pitch of 1.55 and SFOV of Ø 50 cm and a pitch of 3.2 and SFOV of Ø 35.4 cm from most body parts and potential combinations (p > 0.05), Most of the image noises and the AI from the images with the pitch of 1.55 were significantly lower than those with the pitch of 3.2 (p < 0.05), and the SNR and CNR from the images with the pitch of 1.55 were higher than those with the pitch of 3.2. There were significant differences in the first-order features and texture features of the head (59.3%, 28.3%), chest (66%, 35.7%), and abdomen (71.6%, 64.7%) (p < 0.05). The subjective image quality was excellent when the pitch was less than 2.0 and gradually decreased with the increasing pitch. In addition, the image quality decreased significantly when the pitch was higher than 3.0 (all k≥0.69), especially in the head and chest.

Conclusions: In the ultrafast, high-pitch turbo FLASH mode of the third-generation DSCT, increasing the pitch and lowering the corresponding SFOV will change the image features and cause more artifacts degrading the image quality. Specific to the clinical needs, decreasing the pitch not only can expand the SFOV but also can improve the image quality.
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http://dx.doi.org/10.1002/acm2.13466DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8664149PMC
December 2021

Phosphorylation of MICAL2 by ARG promotes head and neck cancer tumorigenesis by regulating skeletal rearrangement.

Oncogene 2022 01 8;41(3):334-346. Epub 2021 Nov 8.

Department of Maxillofacial and Otorhinolaryngology Oncology and Department of Head and Neck Oncology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin, China.

The actin cytoskeletal architecture provides the structural underpinnings for crucial cellular behaviors. In cancer cells, changes in the actin cytoskeleton may serve as prerequisites for proliferation, invasion, and metastatic dissemination. However, the underlying mechanisms remain largely unknown. Here, we show that MICAL2, which is increased in head and neck squamous cell carcinoma (HNSCC) and inversely associated with patient survival, promotes HNSCC growth, invasion, and migration. MICAL2 serves as a flavoprotein monooxygenase and directly induces actin filament depolymerization by specifically oxidizing the methionine 44 and 47 residues of F-actin. The kinase ARG interacts with MICAL2 and augments MICAL2-mediated actin disassembly. Direct phosphorylation assay and mass spectrometry confirmed that ARG phosphorylates MICAL2 at Tyr445, Tyr463, and Tyr488. Substitution of the Tyr445 or Tyr463 residue of purified recombinant MICAL2-redox with phenylalanine (generating a non-phosphorylatable mutant) abolishes the enhanced MICAL2-mediated F-actin disassembly induced by ARG. Consistently, ectopic expression of non-phosphorylatable MICAL2 mutants (MICAL2 and MICAL2, not MICAL2) failed to ameliorate HNSCC cell growth, whereas expression of wild-type MICAL2 or MICAL2 rescued the impaired proliferation induced by MICAL2 knockdown. Moreover, CCG-1423, an inhibitor of MICAL2, was shown to inhibit HNSCC cell proliferation, invasion, and migration. Taken together, our findings indicate that phosphorylation of MICAL2 at Tyr445 and Tyr463 by ARG mediates F-actin disassembly and promotes HNSCC progression.
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http://dx.doi.org/10.1038/s41388-021-02101-zDOI Listing
January 2022

Aesthetic Correction of Mild-to-Moderate Blepharoptosis Among Asians: The Bridge Technique.

Ophthalmol Ther 2022 Feb 6;11(1):151-160. Epub 2021 Nov 6.

Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine, 639 Zhizaoju Road, Shanghai, 200011, China.

Introduction: Although several surgical methods have been introduced to treat mild-to-moderate blepharoptosis, including levator-based techniques such as Müller muscle-conjunctival resection, few complications and better functional and aesthetic outcomes remain elusive for plastic surgeons. Hence, this study aimed to provide a new technique (bridge technique) using the levator aponeurosis-Müller's muscle flap to achieve optimal blepharoptosis correction for function and aesthetics among Asians.

Methods: From January 2019 to May 2020, this new technique was performed on 157 consecutive patients with mild-to-moderate blepharoptosis. Our technique was based on the anchor of the levator complex to the tarsus using mattress stitches and three-layer fixation, which provided a reliable motion transmitter for elevating the upper eyelid. The patients' medical records and photographs were reviewed 12 months postoperatively to assess the margin reflex distance 1 (MRD1), incidence of complications, and aesthetic outcomes.

Results: The average preoperative and postoperative MRD1 measured 1.2 ± 0.31 mm and 3.7 ± 0.34 mm, respectively. A significant difference was observed between the preoperative and postoperative distance values (p < 0.001). In the aesthetic evaluation, the grading was good, fair, and poor for 142 (90.4%) patients, 10 (6.4%) patients, and 5 (3.2%) patients, respectively. Complications included undercorrection in 12 (5.2%) cases and overcorrection in nine (3.9%) cases, and no cases of residual lagophthalmos were recorded.

Conclusion: Mild-to-moderate blepharoptosis among Asians can be corrected effectively using this new technique. However, the long-term outcomes of this procedure should be explored.
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http://dx.doi.org/10.1007/s40123-021-00417-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8770775PMC
February 2022

ADAURA, a Bridge Connecting Disease Control with Long-term Survival.

Sci Prog 2021 10;104(4):368504211050289

Key Laboratory of Respiratory Disease of Zhejiang Province, Department of Respiratory and Critical Care Medicine, 89681Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.

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http://dx.doi.org/10.1177/00368504211050289DOI Listing
October 2021
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