Publications by authors named "Rui Jiang"

696 Publications

Natural polymer-based scaffolds for soft tissue repair.

Front Bioeng Biotechnol 2022 19;10:954699. Epub 2022 Jul 19.

School of Engineering, Westlake University, Hangzhou, Zhejiang.

Soft tissues such as skin, muscle, and tendon are easily damaged due to injury from physical activity and pathological lesions. For soft tissue repair and regeneration, biomaterials are often used to build scaffolds with appropriate structures and tailored functionalities that can support cell growth and new tissue formation. Among all types of scaffolds, natural polymer-based scaffolds attract much attention due to their excellent biocompatibility and tunable mechanical properties. In this comprehensive mini-review, we summarize recent progress on natural polymer-based scaffolds for soft tissue repair, focusing on clinical translations and materials design. Furthermore, the limitations and challenges, such as unsatisfied mechanical properties and unfavorable biological responses, are discussed to advance the development of novel scaffolds for soft tissue repair and regeneration toward clinical translation.
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http://dx.doi.org/10.3389/fbioe.2022.954699DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343850PMC
July 2022

CS-CO: A Hybrid Self-Supervised Visual Representation Learning Method for H&E-stained Histopathological Images.

Med Image Anal 2022 Jul 20;81:102539. Epub 2022 Jul 20.

Ministry of Education Key Laboratory of Bioinformatics, Bioinformatics Division, Beijing National Research Center for Information Science and Technology, Department of Automation, Tsinghua University, Beijing 100084, China. Electronic address:

Visual representation extraction is a fundamental problem in the field of computational histopathology. Considering the powerful representation capacity of deep learning and the scarcity of annotations, self-supervised learning has emerged as a promising approach to extract effective visual representations from unlabeled histopathological images. Although a few self-supervised learning methods have been specifically proposed for histopathological images, most of them suffer from certain defects that may hurt the versatility or representation capacity. In this work, we propose CS-CO, a hybrid self-supervised visual representation learning method tailored for H&E-stained histopathological images, which integrates advantages of both generative and discriminative approaches. The proposed method consists of two self-supervised learning stages: cross-stain prediction (CS) and contrastive learning (CO). In addition, a novel data augmentation approach named stain vector perturbation is specifically proposed to facilitate contrastive learning. Our CS-CO makes good use of domain-specific knowledge and requires no side information, which means good rationality and versatility. We evaluate and analyze the proposed CS-CO on three H&E-stained histopathological image datasets with downstream tasks of patch-level tissue classification and slide-level cancer prognosis and subtyping. Experimental results demonstrate the effectiveness and robustness of the proposed CS-CO on common computational histopathology tasks. Furthermore, we also conduct ablation studies and prove that cross-staining prediction and contrastive learning in our CS-CO can complement and enhance each other. Our code is made available at https://github.com/easonyang1996/CS-CO.
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http://dx.doi.org/10.1016/j.media.2022.102539DOI Listing
July 2022

Regulates Bovine Myoblasts Proliferation and Differentiation via the miR-411a/ Axis.

J Agric Food Chem 2022 Aug 2. Epub 2022 Aug 2.

College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Animal Genetics, Breeding and Reproduction, Yangling, Shaanxi 712100, China.

Circular RNA (circRNA) is endogenous noncoding RNA found throughout the eukaryotic genome. It regulates several biological activities at the transcription or post-transcription level. However, the underlying function of circRNA in bovine skeletal muscle development remains unknown. Here, we identified a novel circRNA, , and investigated its function and mechanism on the proliferation and differentiation of bovine myoblasts. At the molecular and cellular levels, could promote bovine myoblasts proliferation and inhibit differentiation. Mechanistically, is expressed in the cytoplasmic of myoblast and was enriched by protein Ago2. acts as a competing endogenous RNA that sponges miR-411a and alleviates the inhibitory effect on its target gene, . miR-411a and were also involved in regulating bovine myoblast proliferation and differentiation. These findings suggest that functions as a competing endogenous RNA and regulates bovine myoblast proliferation and differentiation through the miR-411a/ axis.
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http://dx.doi.org/10.1021/acs.jafc.1c08167DOI Listing
August 2022

Mechanical response and in-situ deformation mechanism of cortical bone materials under combined compression and torsion loads.

PLoS One 2022 27;17(7):e0271301. Epub 2022 Jul 27.

School of Engineering, Anhui Agricultural University, Hefei, People's Republic of China.

Bone fracture is an extremely dangerous health risk to human. Actually, cortical bone is often subjected to the complicated loading patterns. The mechanical properties and deformation mechanism under the complicated loading pattern could provide a more precise understanding for the bone fracture. For this purpose, the mechanical response and multi-scale deformation mechanism of cortical bone material were investigated by in-situ experimental research using the compression-torsion coupling loads as an example. It was found that the torsion strength and shear modulus all decreased under the compression-torsion coupling loads than single torsion load. This indicated bone would suffer greater risk of fracture under the compression-torsion coupling loads. Based on in-situ observation, it was found that the rapid reduction of the anisotropy of bone material under the compression load was the potential influencing factor. Because of the redistribution of the principal strain and the variations of cracks propagation, the comprehensive fracture pattern containing both transverse and longitudinal fracture was shown under the coupling loads, and finally resulted in the reduction of the torsion properties. This research could provide new references for researches on mechanical properties of cortical bone material under complicated loading patterns.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0271301PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9328520PMC
July 2022

Screening and Research on Skin Barrier Damage Protective Efficacy of Different Mannosylerythritol Lipids.

Molecules 2022 Jul 21;27(14). Epub 2022 Jul 21.

Research Center of Traditional Chinese Medicine, The Affiliated Hospital to Changchun University of Chinese Medicine, Changchun 130021, China.

Mannosylerythritol lipids (MELs) may prevent skin barrier damage, although their protective mechanisms and active monomeric constituents remain unclear. Here, three MELs were extracted from cultures containing fermented olive oil then purified using silica gel-based column chromatography and semipreparative HPLC. All three compounds (MEL-A, MEL-B, MEL-C) were well separated and stable, and reliable materials were used for NMR and HRESIMS chemical structure determinations and for assessing MELs' protective effects against skin damage. Notably, MEL-B and MEL-C effectively protected HaCaT cells from UVB-induced damage by upregulating the contents of filaggrin (FLG) and transglutaminase-1 (TGM1), as determined via ELISA. Moreover, MEL-B treatment (20 μg/mL) of UVB-irradiated HaCaT cells led to the upregulation of both the expression of mRNA genes and the key proteins FLG, LOR, and TGM1, which are known to be decreased in damaged skin cells. Additionally, histopathological analysis results revealed a markedly reduced intracellular vacuolation and cell damage, reflecting improved skin function after MEL-B treatment. Furthermore, immunofluorescence results revealed that MEL-B protected EpiKutis three-dimensional cultured human skin cells from sodium dodecyl sulfate-induced damage by up-regulating FLG, LOR, and TGM1 expression. Accordingly, MELs' protection against skin barrier damage depended on MEL-B monomeric constituent activities, thus highlighting their promise as beneficial ingredients for use in skin-care products.
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http://dx.doi.org/10.3390/molecules27144648DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320248PMC
July 2022

Pre-BMI and Lipid Profiles in Association with the Metabolic Syndrome in Pregnancy with Advanced Maternal Age.

Contrast Media Mol Imaging 2022 9;2022:4332006. Epub 2022 Jul 9.

Department of Endocrinology, Cangzhou Central Hospital, Cangzhou, China.

We aimed to explore the association of BMI in pre-pregnant women with metabolic syndrome in pregnancy in advanced maternal age. A total of 229 maternal women and 536 maternal women participated in this study. Pregnancy women underwent a 75 g-oral glucose tolerance test and maternal lipid profile test between 24 and 28 weeks. Data about biological and sociodemographic characteristics were recorded for each case. The metabolic equivalent (Met) was 9.6% in the maternal age ≥35 group, 5.4% in the age 20-34 group ( = 0.027), and 6.7% in all pregnant women. Results also demonstrated that gestational diabetes mellitus (GDM) and MetS were more likely to appear in the maternal age ≥35 years group than the maternal age 20-34 years group (41.5% vs. 30.6%;  = 0.001, 9.6% vs. 5.4%,  = 0.027). Risk for preterm delivery and eclampsia were increased with raised MetS (RR 3.434 and RR 1.800); MetS in women aged ≥35 years had the largest area under the curve (AUC) (AUC 0.925, 95% CI 0.885-0.965), and its optimal cutoff point was ≥24.998 kg/m, and the optimal cutoff point for total cholesterol (TC) (AUC 0.686, 95% CI 0.571-.802) predicting MetS was ≥4.955 mmol/l. MetS in pregnancy are associated with the occurrence of preterm delivery and eclampsia, and pre-BMI and TC can predict MetS in the maternal age ≥35 group.
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http://dx.doi.org/10.1155/2022/4332006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9288333PMC
July 2022

Association between thyroid function and comorbid anxiety in first-episode and drug naïve patients with major depressive disorder.

Eur Arch Psychiatry Clin Neurosci 2022 Jul 18. Epub 2022 Jul 18.

CAS Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, 16 Lincui Road, Chaoyang District, Beijing, 100101, China.

Objective: Existing studies have shown that thyroid dysfunction is associated with depression. However, its role in major depressive disorder (MDD) with comorbid anxiety remains unclear. The main purpose of this study was to compare thyroid function in a large sample of first episode drug naïve (FEDN) MDD patients with and without anxiety.

Methods: This cross-sectional study examined 1718 outpatients who were drug-naïve and diagnosed as MDD at first episode. Socio-demographic and clinical data, as well as thyroid function-related parameters, including free thyroxine (FT4), free triiodothyronine (FT3), thyroid-stimulating hormone (TSH), thyroid peroxidase antibodies (TPOAb) and anti-thyroglobulin (TGAb), were evaluated. The Hamilton Depression Rating Scale (HAMD), Hamilton Anxiety Rating Scale (HAMA) and the positive subscale of the Positive and Negative Syndrome Scale (PANSS) were used to evaluate depressive, anxiety and psychotic symptoms, respectively.

Results: Compared to MDD patients without anxiety, MDD patients with anxiety were more likely to have more suicide attempts and psychotic symptoms, as well as higher serum levels of TSH, TPOAb and TGAb (all p < 0.001). Among patients with abnormally elevated serum TSH, TPOAb, and TGAb, 83.5% (872/1044), 89.3% (391/438) and 89.6% (266/297) had comorbid anxiety disorders, respectively. The odds ratio between patients with comorbid and without comorbid anxiety was 1.657 (95% CI 1.304-2.105) for elevated TSH levels, 1.943 (95% CI 1.444-2.613) for elevated TGAb levels, and 2.448 (95% CI 1.760-3.403) for elevated TPOAb levels. Furthermore, multivariable linear analysis showed that elevated TSH and TGAb were significant predictors of anxiety in MDD patients.

Conclusions: Our results suggest that comorbid anxiety in FEDN MDD patients is positively associated with elevated TSH and TGAb levels, which may be promising biomarkers of comorbid anxiety in MDD patients. Clinical treatment of impaired thyroid function may be useful for comorbid anxiety in MDD patients.
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http://dx.doi.org/10.1007/s00406-022-01457-xDOI Listing
July 2022

Treatment of long-term endophthalmitis developing after intraocular lens implantation in children: a retrospective study.

BMC Ophthalmol 2022 Jul 12;22(1):301. Epub 2022 Jul 12.

Department of Ophthalmology, Eye and ENT Hospital of Fudan University, 83# Fenyang Road, Shanghai, China.

Background: To discussed the risk factor and the management of vitrectomy for long-term endophthalmitis developing after intraocular lens (IOL) implantation in children.

Methods: We retrospectively investigated the clinical characteristics and surgical outcomes of long-term endophthalmitis developing after IOL implantation in children.

Results: Four eyes of four children were included in the study. The mean time to endophthalmitis development after IOL implantation was 3.0 ± 0.8 years. The corneal or scleral sutures may have been caused the infection. All 4 patients underwent vitrectomy and received intravitreal antibiotics with or without IOL removal. At the last follow-up, the outcomes were satisfactory; the fundus was clear, the retina remained attached, the visual acuity improved, and there were no severe complications.

Conclusions: The use of scleral sutures and the exposure of conceal sutures may induce the onset of long-term endophthalmitis after IOL implantation. Complete vitrectomy and appropriate use of antibiotics are effective in the treatment of long-term endophthalmitis developing after IOL implantation in children.
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http://dx.doi.org/10.1186/s12886-022-02519-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277780PMC
July 2022

Corrigendum: Enhanced Heterogeneous Fenton Degradation of Organic Pollutants by CRC/FeO Catalyst at Neutral pH.

Front Chem 2022 14;10:939407. Epub 2022 Jun 14.

A Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Institute of Environmental Research at Greater Bay, Ministry of Education, Guangzhou University, Guangzhou, China.

[This corrects the article DOI: 10.3389/fchem.2022.892424.].
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http://dx.doi.org/10.3389/fchem.2022.939407DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9237646PMC
June 2022

Icariin inhibits the formation of mitochondria-associated membranes (MAMs) and improves erectile function in rats treated with prostate radiation.

Andrology 2022 Jun 28. Epub 2022 Jun 28.

Department of Urology, The Affiliated Hospital of Southwest Medical University, Luzhou, China.

Background: Erectile function is usually impaired after radiation therapy in prostate cancer patients. eNOS is a key enzyme in the process of erection. Mitochondria-associated membranes (MAMs) are closely contacted with the production and bioactivity of eNOS.

Objective: To study the mechanism of icariin improves the erectile function of rats treated with prostate radiation by controling the expression of MAMs in penile corpus cavernosum.

Methods: Twenty 8-week-old healthy male SD rats were randomized to four groups: control group, radiation therapy (RT) group, icariin (10 mg/kg/d gavage) group, and RT + icariin (10 mg/kg/d gavage) group (n = 5). In RT group and RT + icariin group, rats were irradiated with X-rays to the prostate region (total dose 37.5 gray; 7.5 gray/day for 5 days). The maximum intracavernous pressure/mean arterial pressure (ICPmax/MAP), NO concentration and the level of IP R1, PACS2, FACL4, nNOS, p-eNOS, and eNOS in rats' penile cavernous tissue was determined 9 weeks after radiation therapy.

Results: Compared with the control group and the RT + icariin group, the ICPmax/MAP of the RT group was remarkably reduced (p < 0.05). The levels of p-eNOS/eNOS, nNOS and the concentration of NO in the penile cavernous tissue of the penis in the RT group were remarkably decreased compared to the control group and the RT + icariin group (p < 0.05). The levels of IP R1, PACS2, and FACL4 in penile cavernous tissue of the RT group were significantly higher than those in the control group and the RT + icariin group (p < 0.05).

Conclusions: After prostate X-ray radiotherapy in rats, the formation of MAMs may be increased by increased expression of IP R1, PACS2, and FACL4 in penile cavernous tissue, resulting in impaired erectile function. Icariin might increase p-eNOS/eNOS and improve erectile function in rats after prostate radiotherapy by inhibiting the expression of IP R1, PACS2, and FACL4.
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http://dx.doi.org/10.1111/andr.13218DOI Listing
June 2022

Identification of Abietane-Type Diterpenoids and Phenolic Acids Biosynthesis Genes in Jepson Through Full-Length Transcriptomic and Metabolomic Profiling.

Front Plant Sci 2022 8;13:919025. Epub 2022 Jun 8.

Center of Chinese Traditional Medicine Resources and Biotechnology, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

() Jepson is a medicinal plant that is frequently used by the Chumash Indians in southern California as a diaphoretic, calmative, diuretic, or antimicrobial agent. Abietane-type diterpenoids (ATDs) and phenolic acids (PAs) are the main bioactive ingredients in . However, few studies have looked into the biosynthesis of ATDs and PAs in . In this study, using metabolic profiling focused on the ATDs and PAs in the roots and leaves of , we found a distinctive metabolic feature with all-around accumulation of ATDs, but absence of salvianolic acid B. To identify the candidate genes involved in these biosynthesis pathways, full-length transcriptome was performed by PacBio single-molecule real-time (SMRT) sequencing. A total of 50 and 40 unigenes were predicted to be involved in ATDs and PAs biosynthesis, respectively. Further transcriptional profile using Illumina HiSeq sequencing showed that the transcriptional variations of these pathways were consistent with the accumulation patterns of corresponding metabolites. A plant kingdom-wide phylogenetic analysis of cytochromes (CYPs) identified two CYP76AK and two CYP76AH subfamily genes that might contribute for the specific ATDs biosynthesis in . We also noticed that the clade VII laccase gene family was significantly expanded in compared with that of , indicating their involvements in the formation of salvianolic acid B. In conclusion, our results will enable the further understanding of ATDs and PAs biosynthesis in and genus.
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http://dx.doi.org/10.3389/fpls.2022.919025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213684PMC
June 2022

Brown adipose tissue activation with ginsenoside compound K ameliorates polycystic ovary syndrome.

Br J Pharmacol 2022 Jun 25. Epub 2022 Jun 25.

Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.

Background And Purpose: Polycystic ovary syndrome (PCOS) is a common metabolic and endocrine disease affecting women of reproductive age. Due to its complex aetiology, there is no currently effective cure for PCOS. Brown adipose tissue (BAT) activity is significantly decreased in PCOS patients, and BAT activation has beneficial effects in animal models of PCOS. Here, we investigated the effect of ginsenoside compound K (CK) in an animal model of PCOS and its mechanism of BAT activation.

Experimental Approach: Primary brown adipocytes, Db/Db mice and dehydroepiandrosterone (DHEA)-induced PCOS rats were used. The core body temperature, oxygen consumption, energy metabolism related gene and protein expression were assessed to identify the effect of CK on overall energy metabolism. Oestrous cycle, serum sex hormone, ovarian steroidogenic enzyme gene expression and ovarian morphology were also evaluated following CK treatment.

Key Results: Our results indicated that CK treatment could significantly protect against body weight gain in Db/Db mice via BAT activation. Furthermore, we found that CK treatment could normalize hyperandrogenism, oestrous cyclicity, normalize steroidogenic enzyme expression and decrease the number of cystic follicles in PCOS rats. Interestingly, as a potential endocrine intermediate, C-X-C motif chemokine ligand-14 protein (CXCL14) was significantly up-regulated following CK administration. In addition, exogenous CXC14 supplementation was found to reverse DHEA-induced PCOS in a phenotypically similar manner to CK treatment.

Conclusion And Implications: In summary, CK treatment significantly activates BAT, increases CXCL14 expression and ameliorates PCOS. These findings suggest that CK might be a potential drug candidate for PCOS treatment.
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http://dx.doi.org/10.1111/bph.15909DOI Listing
June 2022

Simultaneous screening and analysis of 155 veterinary drugs in livestock foods using ultra-high performance liquid chromatography tandem quadrupole linear-ion-trap mass spectrometry.

Food Chem 2022 Nov 20;393:133260. Epub 2022 May 20.

AB SCIEX, Analytical Instrument Trading Co, Beijing 100015, China.

Veterinary drugs are widely used to improve the health and growth of livestock. The supervision of these residues is necessary to ensure food safety. A high-throughput method based on Oasis PRiME HLB with solid phase extraction for simultaneous qualitative and quantitative analysis of 155 veterinary drugs in livestock foods was developed by the ultra-high performance liquid chromatography tandem quadrupole linear-ion-trap mass spectrometry (UHPLC-QTRAP-MS). The limits of detection and quantification ranged from 0.5 μg/kg to 5 μg/kg and 2 μg/kg to 20 μg/kg, respectively. For over 85% of the analytes, the recoveries were between 60% and 120%. The positive simulated samples perfectly matched with a purity fit value over 70% from the self-built library. The screening results of UHPLC-QTRAP-MS were almost consistent with UHPLC tandem quadrupole-exactive orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap/MS). The evaluated UHPLC-QTRAP-MS method was powerful and reliable for the screening and quantification of veterinary drugs in real samples.
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http://dx.doi.org/10.1016/j.foodchem.2022.133260DOI Listing
November 2022

Mesenchymal stem cells-derived exosomes prevent sepsis-induced myocardial injury by a CircRTN4/miR-497-5p/MG53 pathway.

Biochem Biophys Res Commun 2022 08 3;618:133-140. Epub 2022 Jun 3.

Zhengzhou Railway Vocational and Technical College, Henan Provincial Engineering Research Center of Natural Drug Extraction and Medical Technology Application, Zhengzhou, 450000, China. Electronic address:

Sepsis is a life-threatening organ function dysfunction featured by stimulated oxidative stress and inflammatory responses, in which about 40%-60% of sepsis patients are accompanied with cardiac dysfunction. Mesenchymal stem cells (MSCs)-derived exosomes exert critical roles in the treatment of multiple diseases through transferring non-coding RNAs. Circular RNA (circRNA) is a novel form of functional RNAs that involves in the progression of multiple cardiac pathological condition. Nevertheless, the function of MSCs-derived exosomal circRTN4 in sepsis-induced myocardial injury is still obscure. Significantly, FISH assay demonstrated the location of circRTN4 in cytoplasm of cardiomyocytes. The expression of circRTN4 was reduced in the cardiac tissues from caecal ligation and puncture (CLP) rats and LPS-treated cardiomyocytes. CircRTN4 could be delivered to cardiomyocytes cells via MSCs-derived exosomes. The cardiac injury and apoptosis were induced in the CLP rats and the treatment of MSCs-derived exosomal circRTN4 relieved the phenotypes. MSCs-derived exosomal circRTN4 notably suppressed the upregulated ROS level in the CLP rats. The activity of SOD and GSH was repressed in CLP rats, in which MSCs-derived exosomal circRTN4 rescued the activity in the rats. The upregulated IL-1β, IL-6, and TNF-α levels in CLP rats were reduced by the treatment of MSCs-derived exosomal circRTN4. MSCs-derived exosomal circRTN4 improved cell survival and suppressed apoptosis of LPS-treated cardiomyocytes. CircRTN4 direct interact with miR-497-5p to upregulate MG53 expression in cardiomyocytes. MSCs-derived exosomal circRTN4 relieves LPS-stimulated cardiomyocyte damage via targeting miR-497-5p/MG53 axis. Therefore, we determine that MSCs-derived exosomes prevent sepsis-induced myocardial injury by a circRTN4/miR-497-5p/MG53 pathway. Our data provides novel insight into the regulatory mechanism by which MSCs-derived exosomal circRTN4 regulates sepsis-induced myocardial injury. MSCs-derived exosomal circRTN4 may be applied as a promising therapeutic approach for sepsis-induced myocardial injury.
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http://dx.doi.org/10.1016/j.bbrc.2022.05.094DOI Listing
August 2022

Cyprinid-specific duplicated membrane TLR5 senses dsRNA as functional homodimeric receptors.

EMBO Rep 2022 Aug 9;23(8):e54281. Epub 2022 Jun 9.

Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, China.

Membrane-embedded Toll-like receptor 5 (TLR5) functions as a homodimer to detect bacterial flagellin. Cyprinid grass carp (Ctenopharyngodon idella) encodes two TLR5 genes, CiTLR5a and CiTLR5b. Here, we show that cyprinid TLR5a and TLR5b homodimers unexpectedly bind the dsRNA analog poly(I:C) and regulate interferon (IFN) response in early endosomes and lysosomes. Although TLR5 homodimers also bind flagellin, an immune response to flagellin is only triggered by TLR5a/b heterodimer. Moreover, we demonstrate that two TLR5 paralogs have opposite effects on antiviral response: CiTLR5a slightly promotes and powerfully maintains, whereas CiTLR5b remarkably inhibits virus replication. We show that the ectodomain of CiTLR5 is required for dsRNA-induced IFN signaling, and we map the key poly(I:C) binding sites to G240 for CiTLR5a and to N547 for CiTLR5b. Furthermore, we reveal that differential N-glycosylation of CiTLR5a/b affects dsRNA-IFN signaling but has no role in flagellin-mediated NF-κB induction, with paralog-specific roles for CiTLR5a-T101 and corresponding CiTLR5b-I99. Moreover, we provide evidence that the ability to sense dsRNA represents a neofunctionalization specific for membrane-bound TLR5 in cyprinid, bridging viral and bacterial immune responses.
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http://dx.doi.org/10.15252/embr.202154281DOI Listing
August 2022

Low androgen level impairs erectile function of rat by regulating the Ng/CaN/AKT/eNOS pathway in penile corpus cavernosum.

Andrology 2022 Jun 9. Epub 2022 Jun 9.

Department of Urology, The Affiliated Hospital of Southwest Medical University, Luzhou, China.

Background: The mechanism by which low androgen status inhibit erectile function has not yet been clearly elucidated. Neurogranin (Ng) is a Ca -sensitive calmodulin binding protein that is expressed in endothelial cells and regulates eNOS function.

Objectives: To investigate whether low androgen status inhibit erectile function by regulating the Ng/CaN/AKT/eNOS pathway in the penile cavernous tissue of rats.

Materials And Methods: Thirty-six 8-week-old male Sprague-Dawley rats were randomly divided into six groups as follows (n = 6): 4-week control group (4w-control), 4-week castration group (4w-cast), 4-week castration+testosterone replacement group (4w-cast+T), 8-week control group (8w-control), 8-week castration group (8w-cast), and 8-week castration+testosterone replacement group (8w-cast+T). Four weeks and eight weeks after surgery, the ratio of the maximum intracavernous pressure/mean arterial pressure (ICPmax/MAP) was examined. The level of NO and the expression of Ng, calcineurin (CaN), AKT, p-AKT(S473), eNOS, and p-eNOS(Ser1177) in the penile cavernous tissue of each group were determined.

Results: Ng and CaN were mainly expressed in the membrane and cytoplasm of endothelial cells and smooth muscle cells in the penile cavernous tissue of rats. The ICPmax/MAP and the concentration of NO in the cast group were significantly lower than those in the control group and cast+T replacement group (p < 0.01). The expression of Ng and the ratios of p-AKT/AKT and p-eNOS/eNOS in the penile cavernous tissue of rats in the cast group were significantly lower than those in the control group and cast+T replacement group (p < 0.01). The expression of CaN in the penile cavernous tissue of rats in the cast group was significantly increased compared with that in the control group and the cast+T replacement group (p < 0.01).

Conclusion: Inhibiting the expression of Ng and subsequently upregulating the expression of CaN in the rat penile cavernous tissue was one of the upstream mechanisms of low androgen status inhibiting erectile function by inhibiting the AKT/eNOS signaling pathway.
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http://dx.doi.org/10.1111/andr.13202DOI Listing
June 2022

AP-1 activation mediates postnatal cardiomyocyte maturation.

Cardiovasc Res 2022 May 31. Epub 2022 May 31.

Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Institute of Stem Cell Research and Clinical Translation, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai 200092, P.R. China.

Aims: Postnatal maturation of mammalian cardiomyocytes proceeds rapidly after birth, with most of the myocytes exiting cell cycle, becoming binucleated, and adopting oxidative phosphorylation as the primary metabolic route. The triggers and transcriptional programs regulating cardiomyocyte maturation have not been fully understood yet. We performed single cell RNA-Seq in postnatal rat hearts in order to identify the important factors for this process.

Methods And Results: Single cell RNA-Seq profiling was performed of postnatal day 1 and day 7 rat hearts, and we found that members of the AP-1 transcription factors showed a transient upregulation in the maturing cardiomyocytes, suggesting their functional involvement in the process. Activating members of the AP-1 family by palmitate or adrenergic stimulation inhibited cardiomyocyte cytokinesis and promoted cardiomyocyte maturation. In contrast, knocking down AP-1 members Atf3 and Jun promoted cardiomyocyte cytokinesis, reduced polyploidy and inhibited maturation. Mechanistically, RNA-Seq results and rescue experiments indicated that AP-1 members activate the expression of fatty acid metabolic genes to promote cardiomyocyte maturation. Finally, intraperitoneal injection of AP-1 inhibitor T-5224 in neonatal mice inhibits cardiomyocyte maturation in vivo.

Conclusion: Our results are the first evidence implicating AP-1 transcription factors in postnatal cardiomyocyte maturation both in vitro and in vivo, which expand our understanding of the molecular mechanism of cardiomyocyte maturation, and may lead to novel therapies to treat congenital heart diseases.

Translational Perspective: Postnatal cardiomyocyte maturation is a crucial process of cardiac development that determines fitness of the adult heart, and can be affected by multiple congenital heart diseases which lead to adult heart conditions. Our finding that AP-1 transcription factors transiently activated by multiple cues such as fatty acid and adrenergic signal promote cardiomyocyte maturation provided novel targets for therapeutic intervention, which may be applied during the narrow time window of postnatal cardiomyocyte maturation to treat congenital heart diseases and limit their impact on the adult heart.
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http://dx.doi.org/10.1093/cvr/cvac088DOI Listing
May 2022

Ocular Manifestations in a Chinese Pedigree of Familial Amyloidotic Polyneuropathy Carrying the Transthyretin Mutation c.401A>G (p.Tyr134Cys).

Genes (Basel) 2022 05 16;13(5). Epub 2022 May 16.

Department of Ophthalmology, Eye & ENT Hospital, Fudan University, Shanghai 200031, China.

Familial amyloid polyneuropathy (FAP) caused by a genetic mutation in transthyretin (TTR) is an autosomal dominant hereditary disease. The retrospective, observational case series study presents the ocular clinicopathological findings of five cases carrying the TTR mutation c.401A>G (p.Tyr134Cys). Multimodal retinal imaging and electrophysiological examination, Congo red staining and immunohistochemical analysis of specimens, and genetic analyses were performed. Cases 1 and 2 were symptomatic with vitreous and retinal amyloid deposition and poor visual recovery. Case 3 had a symptomatic vitreous haze in the left eye with good postoperative visual recovery. The right eye of case 3 and the eyes of cases 4 and 5 were asymptomatic. Thicker retinal nerve fiber layer, retinal venous tortuosity with prolonged arteriovenous passage time on fluorescein angiography and retinal dysfunction detected by multifocal electroretinogram occurred even in asymptomatic eyes. Moreover, the internal limiting membrane from patients with FAP was stained positive for Congo red and transforming growth factor-β1. The results highlight the amyloid deposition of mutant TTR in the optic disc and retina, even in the asymptomatic stage. The deposited amyloid leads to increased resistance to venous return and retinal functional abnormalities. Therefore, careful follow-up of structural and functional changes in the retina is needed, even in asymptomatic patients with FAP.
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http://dx.doi.org/10.3390/genes13050886DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9140613PMC
May 2022

hECA: The cell-centric assembly of a cell atlas.

iScience 2022 May 28;25(5):104318. Epub 2022 Apr 28.

MOE Key Lab of Bioinformatics, Bioinformatics Division of BNRIST and Department of Automation, Tsinghua University, Beijing 100084, China.

The accumulation of massive single-cell omics data provides growing resources for building biomolecular atlases of all cells of human organs or the whole body. The true assembly of a cell atlas should be cell-centric rather than file-centric. We developed a unified informatics framework for seamless cell-centric data assembly and built the human Ensemble Cell Atlas (hECA) from scattered data. hECA v1.0 assembled 1,093,299 labeled human cells from 116 published datasets, covering 38 organs and 11 systems. We invented three new methods of atlas applications based on the cell-centric assembly: "" cell sorting for targeted data retrieval with customizable logic expressions, "quantitative portraiture" for multi-view representations of biological entities, and customizable reference creation for generating references for automatic annotations. Case studies on agile construction of user-defined sub-atlases and "" investigation of CAR-T off-targets in multiple organs showed the great potential enabled by the cell-centric ensemble atlas.
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http://dx.doi.org/10.1016/j.isci.2022.104318DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9114628PMC
May 2022

Do you remember if you have olfactory dreams? A content analysis of LOFTER and a questionnaire survey conducted in China.

Physiol Behav 2022 08 18;252:113849. Epub 2022 May 18.

Chemical Senses and Mental Health Laboratory, Department of Psychology, School of Public Health, Southern Medical University, No. 1023 Shatainan Road, Guangzhou, Guangdong 510515, China. Electronic address:

Objectives: Dreams often involve visual and auditory sensations, but olfactory experiences have not received the same amount of attention. This study explores the prevalence and content of olfactory dreams in China and investigates the relationship between olfactory imagery, olfactory significance, and olfactory dreamers.

Methods: In the first part of the study, 4302 dream records from LOFTER were screened and classified to preliminarily identify the prevalence and content of olfactory dreams. In the second part, 718 participants completed an online questionnaire about olfactory dreams, imagery, and significance.

Results: The prevalence of olfactory dreams in the diary dream entries and questionnaire survey participants were 3.95% and 18.70%, respectively. Instances in which odors appeared in dreams were more positive than negative and were mainly related to food, burning and smoke, body odor, nature, and certain environments and objects. Moreover, individuals with olfactory dreams showed better olfactory imagery, and stronger olfactory significance.

Conclusions: Olfactory sensations occurred in the dreams of Chinese individuals, but their prevalence was very low. Most of the odors that emerged in these dreams were ones that the dreamers were familiar with in their daily lives.
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http://dx.doi.org/10.1016/j.physbeh.2022.113849DOI Listing
August 2022

Effect of low androgen levels on transient receptor potential channels expression in rat penile corpus cavernosum tissue and its relationship with erectile function.

Andrologia 2022 Sep 20;54(8):e14477. Epub 2022 May 20.

Department of Urology, the Affiliated Hospital of Southwest Medical University, Luzhou, China.

The exact mechanism by which testosterone deficiency causes ED has not yet been elucidated. TRPC is involved in the process of smooth muscle cell contraction and relaxation. The effect of androgens on TRPCs and their relationship with erectile function are currently unclear. Thirty male SD rats were randomly divided into six groups: control group, castration group, castration + testosterone (T) group (cast + T), control + transfection group (control + trans), control + empty transfection group and castration + transfection group (cast + trans). The transfection group rats were given with lentivirus (1 × 10 TU/mL, 15 μl) carrying the siRNA targeting TRPC4 gene in the rat penile cavernous tissue at 4 weeks after castration. The tests were performed at 5 weeks after castration. Comparing the cast group with the control, the ICPmax/MAP, p-eNOS/eNOS and NO levels in the rat penile tissue were significantly lower (p < 0.01) and the level of TRPC3, TRPC4 and TRPC6 in the rat penile tissue was significantly increased (p < 0.01). When the cast + trans group was compared to the cast group, ICPmax/MAP was markedly higher (p < 0.05), and the level of the TRPC4 was remarkably lower (p < 0.05). Low androgen levels might inhibit an erectile function through up-regulation of the expression of TRPC3, TRPC4 and TRPC6 in rat penile cavernous tissue. Inhibition the level of TRPC4 in rat penile tissue may improve the erectile function in low androgen levels.
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http://dx.doi.org/10.1111/and.14477DOI Listing
September 2022

Hic12, a novel acidic matrix protein promotes the transformation of calcite into vaterite in Hyriopsis cumingii.

Comp Biochem Physiol B Biochem Mol Biol 2022 Aug-Sep;261:110755. Epub 2022 May 14.

School of Life Science, Shaoxing University, Huancheng West Road 508, Shaoxing 312000, PR China. Electronic address:

Shell acidic matrix proteins are widely considered to be essential for shell formation given their low affinity and high loading for calcium ion. In the present study, a novel matrix protein, hic12, was isolated from the mantle of Hyriopsis cumingii. High expression in tissue and positive signals with in situ hybridization were detected in the mantle center and mantle pallium, indicating that hic12 mainly participated in the biomineralization of the shell nacreous layer. The expression pattern of hic12 in the pearl sac during early pearl formation indicated that it was involved in pearl biomineralization. Moreover, the recombinant protein, rGST-Hic12, was successfully expressed and purified. The addition of rGST-Hic12 could accelerate the calcium carbonate deposition rate, change the morphology of crystals, and promote the conversion of calcite to vaterite. These results may provide new insights into the molecular mechanisms of aragonite mollusk shell formation.
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http://dx.doi.org/10.1016/j.cbpb.2022.110755DOI Listing
May 2022

Pantothenate protects against obesity via brown adipose tissue activation.

Am J Physiol Endocrinol Metab 2022 07 16;323(1):E69-E79. Epub 2022 May 16.

Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.

Brown adipose tissue (BAT) is the primary site of adaptive thermogenesis, which is involved in energy expenditure and has received much attention in the field of obesity treatment. By screening a small-molecule compound library of drugs approved by the Food and Drug Administration, pantothenic acid was identified as being able to significantly upregulate the expression of uncoupling protein 1 (UCP1), a key thermogenic protein found in BAT. Pantothenate (PA) treatment decreased adiposity, reversed hepatic steatosis, and improved glucose homeostasis by increasing energy expenditure in C57BL/6J mice fed a high-fat diet. PA also significantly increased BAT activity and induced beige adipocytes formation. Mechanistically, the beneficial effects were mediated by UCP1 because PA treatment was unable to ameliorate obesity in UCP1 knockout mice. In conclusion, we identified PA as an effective BAT activator that can prevent obesity and may represent a promising strategy for the clinical treatment of obesity and related metabolic diseases. PA treatment effectively and safely protected against obesity via the BAT-UCP1 axis. PA has therapeutic potential for treating obesity and type II diabetes.
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http://dx.doi.org/10.1152/ajpendo.00293.2021DOI Listing
July 2022

Cardiac involvement in patients recovering from Delta Variant of COVID-19: a prospective multi-parametric MRI study.

ESC Heart Fail 2022 Aug 13;9(4):2576-2584. Epub 2022 May 13.

Department of Radiology, Guangzhou Eighth People's Hospital, Guangzhou Medical University, Guangzhou, China.

Aims: The cardiac injury and sequelae of Delta Variant of coronavirus disease 2019 (COVID-19) remain unknown. This study aimed to evaluate the presence of cardiac involvement in patients recovering from Delta Variant of COVID-19 based on multi-parametric cardiac magnetic resonance imaging (MRI).

Methods And Results: We prospectively assessed patients recovering from Delta Variant of COVID-19 using multi-parametric cardiac magnetic resonance imaging (MRI) between June 2021 and July 2021. Comparison was made with 25 healthy controls. Forty-four patients (median age 51 years, 28 women) recovering from Delta Variant were recruited and had a median time of 35 days between diagnosis and cardiac MRI. There were no patients with chest pain (0/44, 0%) and high sensitivity cardiac troponin T troponin elevation (median levels 2.20 pg/mL, IQR levels 0.85-4.40 pg/mL). Regarding the cardiac imaging findings, a total of 14 (32%) patients presented cardiac tissue feature abnormalities, and a total of 9 (20%) patients had a myocarditis-like injury based on cardiac MRI 2018 Lake Louise criteria. When we further assessed the T1 and T2 mapping values for of patients' individual, abnormal raised global native T1, T2, and extracellular volume were seen in 6 (14%), 6 (14%), and 4 (9%) patients, respectively. Comparing with controls, the patients had lower LV global longitudinal strain and (-22.2 ± 2.8% vs. -24.6 ± 2.0%, P < 0.001) and global circumferential strain (-20.7 ± 6.8% vs. -24.3 ± 2.9%, P = 0.014), but higher global native T1 (1318.8 ± 55.5 ms vs. 1282.9 ± 38.1 ms, P = 0.006). Four (9%) patients presented myocardial late gadolinium enhancement with subepicardial pattern mostly common seen, and two (5%) patients presented pericardial enhancement.

Conclusions: The cardiac MRI could detect subclinical functional and myocardial tissue characteristic abnormalities in individuals who were recovering from Delta Variant without cardiac-related clinical findings. The native T1 mapping and strain imaging may be a sensitive tool for the noninvasive detection of a subset of patients who are at risk for cardiac sequelae and more prone to myocardial damage in survivors with Delta Variant.
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http://dx.doi.org/10.1002/ehf2.13971DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9288765PMC
August 2022

A lung adenocarcinoma patient harboring MET c. 3028 + 2 T >A variant sensitive to crizotinib treatment.

Invest New Drugs 2022 Aug 12;40(4):870-873. Epub 2022 May 12.

The Medical Department, The State Key Lab of Translational Medicine and Innovative Drug Development, Jiangsu Simcere Diagnostics Co., Ltd; Nanjing Simcere Medical Laboratory Science Co., Ltd; Jiangsu Simcere Diagnostics Co., Ltd; Xuanwu District, Building 5, No. 699-18 Xuanwu Avenue, Nanjing, Jiangsu Province, China.

Several studies have reported that patients harboring MET-ex14 skipping benefit from MET tyrosine kinase inhibitors (TKIs) such as crizotinib, however, the overall response of crizotinib was 32% in these patients. Therefore, the clinical outcome of patients harboring different MET 14 skipping subtypes are worthy to be concern. Based on NGS analysis, we described a lung adenocarcinoma patient harboring a MET c.3028 + 2 T > A mutation which was predicted to lead to MET-ex14 skipping. Moreover, we performed IHC and qPCR to verify this variant. Then the patient treated with crizotinib and achieved good therapeutic effect. This mutation is firstly verified not only by multiple methodologies, but also by clinical effect. Our finding expands the spectrum of MET 14 exon skipping variant and maybe offer available application basis of MET inhibitor to patients harboring MET c. 3028 + 2 T > A/C/G. Importantly, targeted NGS analysis could improve detection of MET alterations in routine practice.
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http://dx.doi.org/10.1007/s10637-022-01252-4DOI Listing
August 2022

Recurrent circadian circuitry regulates central brain activity to maintain sleep.

Neuron 2022 07 6;110(13):2139-2154.e5. Epub 2022 May 6.

Department of Neurobiology, Department of Neurology of Sir Run Run Shaw Hospital, Zhejiang University School of Medicine 310058 Hangzhou, China; NHC and CAMS Key Laboratory of Medical Neurobiology, MOE Frontier Science Center for Brain Research and Brain-Machine Integration, School of Brain Science and Brain Medicine, Zhejiang University, 310058 Hangzhou, China. Electronic address:

Animal brains have discrete circadian neurons, but little is known about how they are coordinated to influence and maintain sleep. Here, through a systematic optogenetic screening, we identified a subtype of uncharacterized circadian DN3 neurons that is strongly sleep promoting in Drosophila. These anterior-projecting DN3s (APDN3s) receive signals from DN1 circadian neurons and then output to newly identified noncircadian "claw" neurons (CLs). CLs have a daily Ca cycle, which peaks at night and correlates with DN1 and DN3 Ca cycles. The CLs feedback onto a subset of DN1s to form a positive recurrent loop that maintains sleep. Using trans-synaptic photoactivatable green fluorescent protein (PA-GFP) tracing and functional in vivo imaging, we demonstrated that the CLs drive sleep by interacting with and releasing acetylcholine onto the mushroom body γ lobe. Taken together, the data identify a novel self-reinforcing loop within the circadian network and a new sleep-promoting neuropile that are both essential for maintaining normal sleep.
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http://dx.doi.org/10.1016/j.neuron.2022.04.010DOI Listing
July 2022

Convergence between germline and somatic mutations in pancreatic neuroendocrine tumors.

Eur J Endocrinol 2022 May 19;187(1):85-90. Epub 2022 May 19.

Department of General Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Science & Peking Union Medical College, Beijing, China.

Objectives: The pancreatic neuroendocrine tumors (PanNETs) are a group of clinically heterogeneous neoplasms. Although previous studies illustrated the somatic mutation pattern for PanNETs, the germline mutation pattern is still unclear. Here, we comprehensively screened the underlying germline mutations in a cohort of multiple endocrine neoplasia type 1 (MEN1)-related and sporadic PanNETs to reveal the characteristics of germline mutation in PanNET patients.

Methods: Patients diagnosed with PanNETs by biopsy or surgical pathology were enrolled in this study. Peripheral blood samples were used for genomic DNA purification and subsequent sequencing. The following sequencing techniques were used and compared for validation: (1) targeted gene capture with a customized panel; (2) whole exome sequencing data from previous study.

Results: A total of 184 PanNET patients were enrolled, including 20 MEN1-related and 164 sporadic cases. In this study, MEN1 mutation rate in MEN1-related PanNETs was 60% (12/20), of which 50% were novel mutation sites. For sporadic PanNETs, the overall germline mutation rate was very low. Besides the rare MEN1 mutation, previously unreported germline variant in DAXX was found in one non-functional PanNET.

Conclusions: This study revealed distinctive germline mutation rates between MEN1-related and sporadic PanNETs. The novel MEN1 mutations contribute to revealing the spectrum of MEN1 mutations in PanNETs. The newly discovered germline variant of DAXX in sporadic PanNET implies a tendency of convergence between germline and somatic mutation genes.
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http://dx.doi.org/10.1530/EJE-21-0893DOI Listing
May 2022

StTINY3 enhances cold-induced sweetening resistance by coordinating starch resynthesis and sucrose hydrolysis in potato.

J Exp Bot 2022 May 2. Epub 2022 May 2.

Chongqing Key Laboratory of Biology and Genetic Breeding for Tuber and Root Crops; Engineering Research Center of South Upland Agriculture, Ministry of Education; Southwest University, Chongqing 400715, People's Republic of China.

The accumulation of reducing sugars in cold-stored tubers, known as cold-induced sweetening (CIS), negatively affects potato processing quality. The starch-to-sugar interconversion pathways that are altered in cold-stored CIS tubers have been elucidated, but the mechanism that regulates them remains largely unknown. This study identified a CBF/DREB transcription factor (StTINY3) that enhances CIS resistance by both activating starch biosynthesis and repressing the hydrolysis of sucrose to reducing sugars in detached cold-stored tubers. Silencing StTINY3 in a CIS-resistant genotype decreased CIS resistance, while overexpressing StTINY3 in a CIS-sensitive genotype increased CIS resistance and altering StTINY3 expression was associated with expression changes in starch resynthesis-related genes. We showed first, that overexpressing StTINY3 inhibited sucrose hydrolysis by enhancing invertase inhibitor StInvInh2 expression, and second, that StTINY3 promoted starch resynthesis by upregulating a large subunit of ADP-glucose pyrophosphorylase StAGPaseL3, and the glucose-6-phosphate transporter StG6PT2. We also provide evidence that StTINY3, is a nucleus-localized transcriptional activator that directly binds to the dehydration-responsive element/CRT cis-element in the promoters of StInvInh2 and StAGPaseL3 using electrophoretic mobility shift assays. Taken together, these findings establish that StTINY3 influences CIS resistance in cold-stored tubers by coordinately modulating the starch-to-sugar interconversion pathways and is a good target for improving potato processing quality.
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http://dx.doi.org/10.1093/jxb/erac171DOI Listing
May 2022

Enhanced Heterogeneous Fenton Degradation of Organic Pollutants by CRC/FeO Catalyst at Neutral pH.

Front Chem 2022 14;10:892424. Epub 2022 Apr 14.

A Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Institute of Environmental Research at Greater Bay, Ministry of Education, Guangzhou University, Guangzhou, China.

FeO-based heterogeneous Fenton catalysts have been widely employed for degrading organic pollutants, however it is challenging to use them in highly efficient and recyclable application in wastewater treatment. In this work, carboxylate-rich carbon (CRC)-modified FeO magnetic particles are prepared by the sol-gel self-combustion method, where CRC is obtained from the carbonization of sodium gluconate. The CRC/FeO catalyst exhibits high heterogeneous Fenton degradation performance. The complete 10 mg L methylene blue (MB) removal is achieved in 180 min under conditions of 10 mM HO and 1.00 g of L CRC/FeO at neutral pH. After five cycles, the structure and morphology of CRC/FeO composites remained unchanged and the catalytic activity also remained unaltered. Moreover, phenol, benzoic acid (BA), sulfamethazine (SMT), and tetracycline (TC) were also degraded in the heterogeneous Fenton reaction using CRC/FeO as a catalyst. The strong coordinating ability of -COOH/ -COO functionalities of CRC formed strong bonds with Fe(II/III) ions on the surfaces of FeO particles, which was conducive to adsorption of organic matter on the surface of the catalyst and promoted the occurrence of heterogeneous Fenton reactions. It was found that CRC/FeO had higher removal rates for the adsorptive exclusions of pollutants, such as TC and MB, whereas there were lower removal rates for phenol, BA, and SMT. This work brings potential insights for development of a novel adsorption-enhanced heterogeneous Fenton reaction for wastewater treatment.
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http://dx.doi.org/10.3389/fchem.2022.892424DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9049183PMC
April 2022

Perioperative Use of Pituitrin after Cardiac Defect Repair in Adult Patients with Severe Pulmonary Hypertension.

Heart Surg Forum 2022 Apr 21;25(2):E277-E283. Epub 2022 Apr 21.

Adult Cardiac Surgery Center, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: Vasopressin can constrict peripheral arteries without constricting the pulmonary artery. Theoretically, vasopressin is suitable for the perioperative treatment of pulmonary hypertension. Few studies have investigated the use of pituitrin (a substitute for vasopressin) after cardiac defect repair surgery. This study aimed to analyze the effect of pituitrin on hemodynamics and to determine whether pituitrin can be used after surgical repair in adult patients with pulmonary arterial hypertension-congenital heart disease (PAH-CHD).

Methods: A pulmonary artery catheter was used in all the patients for hemodynamic monitoring. Hemodynamic parameters were recorded before and at 0.5 h, 1 h, 6 h, 12 h and 24 h after pituitrin administration. The changes in the hemodynamic parameters before and after pituitrin use were analyzed through repeated measures analysis of variance.

Results: A total of 110 patients with PAH-CHD underwent repair surgery; 23 patients were included in further analysis, including 11 with atrial septal defect, 9 with ventricular septal defect, and 3 with patent ductus arteriosus. Ten (43.5%) were men, with a mean age of 29.4 ± 6.8 years. Hemodynamic parameters before and after the oxygen test were as follows: radial artery oxygen saturation, 91.5% ± 4.4 vs. 97.9 ± 2.4%; pulmonary vascular resistance, 10.5 ± 1.8 Wood units (wu) vs. 5 ± 1.2 wu; systemic-pulmonary blood flow ratio (QP/QS), 1.1 ± 0.2 vs. 2.1 ± 0.9. With prolonged use, the systolic blood pressure of the radial artery increased significantly (P = 0.001), that of the pulmonary artery decreased significantly (P = 0.009), and RP/s decreased significantly (P < 0.001).

Conclusion: Pituitrin as an alternative to vasopressin can increase arterial pressure, decrease pulmonary artery pressure, and reduce the pulmonary artery pressure/arterial pressure ratio after repair surgery in adult patients with PAH-CHD.
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http://dx.doi.org/10.1532/hsf.4569DOI Listing
April 2022
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