Publications by authors named "Rui Han"

366 Publications

Stenotrophomonas strain CPCC 101271, an intestinal lifespan-prolonging bacterium for Caenorhabditis elegans that assists in host resistance to "Bacillus nematocida" colonization.

Arch Microbiol 2021 Jul 14. Epub 2021 Jul 14.

College of Life Science and Agricultural Engineering, Nanyang Normal University, 1638 Wolong Road, Nanyang, 473061, Henan, China.

The soil-dwelling, opportunistic pathogenic bacterium "Bacillus nematocida" B16 exhibits strong killing activities against a variety of pathogenic nematodes via a "Trojan horse" mechanism that can kill worm species like Caenorhabditis elegans. The bacterial strain CPCC 101271 was previously isolated from the intestines of C. elegans that were recovered from natural habitats and can serve as a probiotic for C. elegans, while also assisting in resistance to infection by the pathogenic strain B16. In this study, the lifespan of C. elegans fed with strain CPCC 101271 cells was extended by approximately 40% compared with that of worms fed with Escherichia coli OP50 cells. In addition, the colonization of C. elegans by the pathogenic bacterium "B. nematocida" B16 was inhibited when pre-fed with strain CPCC 101271. Metagenomic sequence analysis of intestinal microbiota of C. elegans fed with strain CPCC 101271 and infected with B16 revealed that pre-feeding worms with CPCC 101271 improved the diversity of the intestinal bacteria. Moreover, community structure significantly varied in coordination with Stenotrophomonas spp. and Bacillus spp. abundances when competition between strains CPCC 101271 and B16 was evaluated. In conclusion, the nematode microbiota strain CPCC 101271 assisted in its host resistance to colonization by the pathogen "Bacillus nematocida" and can also promote life span-prolongation in C. elegans. These results underscore that understanding the interactions between C. elegans microbiota and pathogens can provide new insights into achieving effective biological control of agricultural pests.
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http://dx.doi.org/10.1007/s00203-021-02467-4DOI Listing
July 2021

First Chinese patient with mental retardation-40 due to a CHAMP1 frameshift mutation: Case report and literature review.

Exp Ther Med 2021 Aug 25;22(2):907. Epub 2021 Jun 25.

Neurology Department of Pediatrics, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450000, P.R. China.

Mental retardation-40 (MRD40) is a rare autosomal dominant neurodevelopmental disorder with a poor prognosis that is caused by a heterozygous mutation in chromosome alignment maintaining phosphoprotein 1 (CHAMP1). It was previously considered a non-syndromic disease due to the lack of specific external features. Only limited international reports describing CHAMP1 mutations are currently available. The present case study was the first to report on a Chinese patient with MRD40. The patient presented with severe global development delay with significant craniofacial dysmorphia. Using trio whole-exome sequencing, a novel frameshift mutation in CHAMP1, NM_032436.2: c.530delCinsTTT, was identified, which expands the spectrum of the known pathogenic variants. The present case report helps to improve the syndromic profile of the rare MRD40 disorder and provides an example for the clinical diagnosis of MRD40.
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http://dx.doi.org/10.3892/etm.2021.10339DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243316PMC
August 2021

Ultrastructural observation of Botrytis cinerea and physical changes in resistant and susceptible grapevines.

Phytopathology 2021 Jul 9. Epub 2021 Jul 9.

Taicheng Road No.3Yangling, China, 712100;

The necrotrophic fungus is a major threat to grapevine cultivation worldwide. Here, a highly-resistant Chinese wild grapevine Rupr 'Shuangyou' (SY) and the susceptible 'Red Globe' (RG) were selected for study, and their pathogenic infection and biochemical responses to were evaluated. The results revealed more trichomes on and a thicker cuticle for leaves of SY than RG under scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Both SEM and TEM also showed that conidial germination, appressorium formation, and hyphal development of were delayed on the leaves of resistant SY. Fewer infected hyphae were also observed in leaves of resistant SY when compared with susceptible RG. The infected leaves of resistant SY harbored higher levels of cellulase and pectinase activity during the early infection stages of at 4 hours post-inoculation (hpi), and higher glucanase and chitinase activity were maintained in the inoculated leaves of SY from 4 hpi through 18 hpi. Lignin was deposited in the infected leaves of susceptible RG but not in resistant SY. Taken together, these results provide insights into the ultrastructural characterizations and physical changes in resistant and susceptible grapevines.
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http://dx.doi.org/10.1094/PHYTO-11-20-0520-RDOI Listing
July 2021

Neuropeptides, Inflammation, Biofilms, and diabetic Foot Ulcers.

Exp Clin Endocrinol Diabetes 2021 Jul 5. Epub 2021 Jul 5.

Department of Endocrinology, The 980th (Bethune International Peace) Hospital of the Joint Logistic Support Force of PLA, China.

A diabetic foot ulcer (DFU) is a serious complication in patients with diabetes mellitus (DM). A DFU is the most common cause of non-traumatic limb amputation, and patients with DFUs have increased mortality rates within 5 years after amputation. DFUs also increase the risk of cardiovascular and cerebrovascular diseases; therefore, with the increasing incidence and prevalence of diabetic foot wounds, DFUs are gradually becoming a major public health problem. The pathophysiology of DFUs is complicated and remains unclear. In recent years, many studies have demonstrated that the pathophysiology of DFUs is especially associated with neuropeptides, inflammation, and biofilms. Neuropeptides, especially substance P (SP) and calcitonin gene-related peptide (CGRP), play an important role in wound healing. SP and CGRP accelerate the healing of cutaneous wounds by promoting neovascularization, inhibiting the release of certain proinflammatory chemokines, regulating macrophage polarization, and so on. However, the expression of SP and CGRP was downregulated in DM and DFUs. DFUs are characterized by a sustained inflammatory phase. Immune cells such as neutrophils and macrophages are involved in the sustained inflammatory phase in DFUs by extracellular traps (NETs) and dysregulated macrophage polarization, which delays wound healing. Furthermore, DFUs are at increased risk of biofilm formation. Biofilms disturb wound healing by inducing a chronic inflammatory response, inhibiting macrophage phagocytosis and keratinocyte proliferation migration, and transferring antimicrobial resistance genes. To understand the relationships among neuropeptides, inflammation, biofilms, and DFUs, this review highlights the recent scientific advances that provide possible pathophysiological insights into the delayed healing of DFUs.
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http://dx.doi.org/10.1055/a-1493-0458DOI Listing
July 2021

The Association Between Blood Pressure and Headache in Postmenopausal Women: A Prospective Hospital-Based Study.

Int J Gen Med 2021 16;14:2563-2568. Epub 2021 Jun 16.

The Affiliated Yantai Yuhuangding Hospital of Medical College, Qingdao University, Yantai, Shandong Province, People's Republic of China.

Background: Headache is the most frequent condition for outpatient patients because of neurological problems, but little is known about predisposing and enabling factors for headache patients.

Aim: To investigate the association between blood pressure (BP) and headache in postmenopausal women.

Methods: The postmenopausal women who were admitted to our hospital from January 2015 to December 2019 were screening according to the criteria. Their systolic BP (SBP), diastolic BP (DBP), pulse pressure (PP) were assessed and the information of age, body mass index, smoking status, caffeine consumption, cholesterol levels, and daily alcohol use were collected. Multiple logistic regression model was established to evaluate the association between BP and headache.

Results: A total of 1571 postmenopausal women were included in the analysis, including 953 headache-free population and 618 headache participants during the studied periods. We found that increasing SBP and PP were associated with the lower occurrence of migraine, tension-type headache (TTH), probable migraine, and unclassified headache (P < 0.05). However, there was a negative association between DBP levels and the new occurrence of overall headache, but we did not find any relations of DBP with any subtypes of headache.

Conclusion: There were negative associations of SBP and PP with new occurrence of headache, especially migraine and TTH, but there is no relationship between DBP and the subsequent development of headache.
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http://dx.doi.org/10.2147/IJGM.S317780DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8216200PMC
June 2021

A mutation of EYA1 gene in a Chinese Han family with Branchio-Oto syndrome.

Medicine (Baltimore) 2021 Jun;100(25):e24691

Department of Prenatal Diagnosis, Reproductive Medicine Center, The First Affiliated Hospital of Xinjiang Medical University.

Abstract: Branchio-Oto (BO) syndrome is one of the common syndromic forms of hearing loss. In this study, we aimed to characterize the clinical and genetic features of BO syndrome in a Chinese deaf family.The proposita in this study was a 29-years-old Chinese female with hearing loss, microtia, anterior concave auricle, and right branchial fistula. The family members agreed to undergo clinical examination. We collected blood samples from 7 family members, including 4 affected by the syndrome. Genomic DNA was extracted and subjected to Sanger sequencing. In addition, bioinformatics software SWISS MODEL was used to predict the protein encoded by EYA transcriptional coactivator and phosphatase 1 (EYA1) gene.Intra-familial consistency can be observed in the clinical phenotypes of BO syndrome in this family. EYA1 c.1627C>T (p.Gln543Ter) mutation was identified as the pathogenic cause in this family.This study reports a mutation associated with BO syndrome in a Chinese Han family. We highlight the utility of genetic testing in the diagnosis of BO syndrome. Thus, we believe that this report would provide a basis for the diagnosis of similar diseases in the future.
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http://dx.doi.org/10.1097/MD.0000000000024691DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8238333PMC
June 2021

Screening and clinical significance of lymph node metastasis-related genes within esophagogastric junction adenocarcinoma.

Cancer Med 2021 Jun 21. Epub 2021 Jun 21.

Department of Thoracic Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Shandong Province, P. R. China.

Background: Despite recent improvements in treatment technologies, such as surgical resection and chemoradiotherapy, the prognosis of patients with esophagogastric junction adenocarcinoma (EJA) remains poor due to early lymph node metastasis. Since few studies have investigated genes associated with lymph node metastasis in EJA, we aimed to screen lymph node metastasis-associated genes and clarify their expression status and prognostic significance in EJA.

Methods: The differential frequency of mutations between carcinoma and para-carcinoma tissues from 199 cases with EJA was detected using targeted next-generation sequencing (tNGS). Following a stratified analysis to determine that gender has no effect on the frequency of gene mutations, lymph node metastasis-related genes, including CDK6, MET, NOTCH1, and LRP1B, were screened, and CDK6 and LRP1B were selected for further study as they displayed significant differences in mutation rates. Differences in their expression status were verified using immunohistochemical (IHC) staining in 18 CDK6- and 17 LRP1B-mutated samples and a randomly matched control group.

Results: tNGS revealed that CDK6 and LRP1B mutation frequencies were significantly different between EJA cases with (N ≥ 1) or without (N = 0) lymph node metastasis. In particular, CDK6 mutation frequency was expected less, whereas that of LRP1B was remarkably higher in cases with stage N0 than in those with stage N ≥ 1. IHC staining confirmed significant differences in CDK6 and LRP1B expression status between the study and control cohorts. Chi-square tests revealed that a high CDK6 expression status correlated significantly with smoking history (p = 0.044), T stage (p = 0.035), N stage (p = 0.000), and advanced TNM stage (p = 0.001) in EJA, whereas a high LRP1B expression status only correlated with BMI (p = 0.013) and N stage (p = 0.000). Furthermore, as confirmed by survival status investigation, a high LRP1B expression status predicted good prognosis, and a high CDK6 expression status was an independent predictor of poor prognosis in patients with EJA.

Conclusions: Taken together, the findings of this study demonstrate that a high CDK6 and LRP1B expression status promotes and inhibits lymph node metastasis in patients with EJA, respectively, suggesting that both CDK6 and LRP1B are significantly potential predictors of lymph node metastasis and prognosis in EJA.
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http://dx.doi.org/10.1002/cam4.4065DOI Listing
June 2021

Potential applications of porous organic polymers as adsorbent for the adsorption of volatile organic compounds.

J Environ Sci (China) 2021 Jul 30;105:184-203. Epub 2021 Jan 30.

Tianjin Key Lab of Indoor Air Environmental Quality Control, School of Environmental Science and Technology, Tianjin University, Tianjin 300350, China.

Volatile organic compounds (VOCs) with high toxicity and carcinogenicity are emitted from kinds of industries, which endanger human health and the environment. Adsorption is a promising method for the treatment of VOCs due to its low cost and high efficiency. In recent years, activated carbons, zeolites, and mesoporous materials are widely used to remove VOCs because of their high specific surface area and abundant porosity. However, the hydrophilic nature and low desorption rate of those materials limit their commercial application. Furthermore, the adsorption capacities of VOCs still need to be improved. Porous organic polymers (POPs) with extremely high porosity, structural diversity, and hydrophobic have been considered as one of the most promising candidates for VOCs adsorption. This review generalized the superiority of POPs for VOCs adsorption compared to other porous materials and summarized the studies of VOCs adsorption on different types of POPs. Moreover, the mechanism of competitive adsorption between water and VOCs on the POPs was discussed. Finally, a concise outlook for utilizing POPs for VOCs adsorption was discussed, noting areas in which further work is needed to develop the next-generation POPs for practical applications.
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http://dx.doi.org/10.1016/j.jes.2021.01.007DOI Listing
July 2021

Alkylated Salicylaldehydes and Prenylated Indole Alkaloids from the Endolichenic Fungus and Their Bioactivities.

J Agric Food Chem 2021 Jun 7;69(23):6524-6534. Epub 2021 Jun 7.

Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry and Pharmacy, Northwest A&F University, Yangling 712100, P. R. China.

Sixteen metabolites, including seven C-alkylated salicylaldehyde derivatives (-) and nine prenylated indole alkaloids (-), three of which are new, namely, asperglaucins A and B ( and ) and neoechinulin F (), were separated from the endolichenic fungus SQ-8. Asperglaucin A () represents an unusual phthalide-like derivative with a benzo[]thiophen-1(3)-one scaffold. All compounds were assessed for antibacterial, antineuroinflammatory, and antioxidant activities. Notably, asperglaucins A and B exhibited potent antibacterial activities against two plant pathogens pv (Psa) and , with an MIC value of 6.25 μM; further SEM analyses illustrated that the possible bacteriostatic mechanisms for compounds and were to alter the external structure of and Psa, and to cause the rupture or deformation of the cell membranes, respectively, and the results suggest that compounds and may serve as potential promising candidates for lead compounds of agrochemical bactericides. Furthermore, compounds and significantly inhibited nitric oxide production with an IC value of ca. 12 μM, and the possible anti-inflammatory mechanisms involved were also studied by molecular docking. Finally, the tested phenolics - showed significant antioxidative effects. Thus, strain SQ-8 represents a novel resource of these bioactive metabolites to be utilized.
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http://dx.doi.org/10.1021/acs.jafc.1c01148DOI Listing
June 2021

Microbial halophilic lipases: A review.

J Basic Microbiol 2021 Jul 7;61(7):594-602. Epub 2021 Jun 7.

College of Bioengineering, Sichuan University of Science and Engineering, Zigong, China.

Microbial lipases are commercially significant due to their versatile catalytic function of hydrolysis triacylglycerol. Among these, lipases from extremophiles are optimal for industrial application. Halophilic microorganisms living in a high salinity environment, such as the ocean, salt lakes, salt wells, and so on, produce halophilic lipases. In recent decades, many remarkable achievements have been made related to the properties and application of halophilic lipases. This review offers information collected over the last decades on halophilic lipase sources as well as advances in production, factors influencing activity, stability under various conditions, structural characteristics, progress in industrial applications such as food flavor modification, biodiesel production, and waste treatment, to provide theoretical and methodological references for the research in this direction.
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http://dx.doi.org/10.1002/jobm.202100107DOI Listing
July 2021

Effects of baicalin capsules combined with α-lipoic acid on nerve conduction velocity, oxidative stress and inflammatory injury in patients with diabetic peripheral neuropathy.

Am J Transl Res 2021 15;13(4):2774-2783. Epub 2021 Apr 15.

Department of Endocrinology, Chongqing Traditional Chinese Medicine Hospital Chongqing, China.

Objective: To observe the effects of baicalin capsules combined with α-lipoic acid on nerve conduction velocity, oxidative stress and inflammatory injury in patients with diabetic peripheral neuropathy.

Methods: A total of 96 patients with diabetic peripheral neuropathy who received treatment in our hospital were divided into the control group (CG) and the research group (SG) by a randomized double-blind method, with 48 cases in each group. In the CG, patients were treated with α-lipoic acid. In the SG, patients were treated with baicalin capsules combined with α-lipoic acid. The clinical efficacy, symptoms (neuropathic subjective symptom questionnaire (TSS) score), nerve conduction velocity, oxidative stress indicators (superoxide dismutase (SOD) and malondialdehyde (MDA)), inflammatory injury indicators (hs-C-reactive protein (CRP), interleukin-6 (IL-6), tumor necrosis factor (TNF-α)) and adverse reactions were compared between the two groups.

Results: The clinical efficacy in the SG was higher than that in the CG (P<0.05). After treatment, the TSS score and the levels of serum SOD, MDA, TNF-α, IL-6 and CRP decreased in both groups, and the SG was lower than the CG (P<0.05). After treatment, the motor conduction velocity (MCV) and sensory conduction velocity (SCV) velocities of the tibial nerve and common peroneal nerve increased in both groups, and the SG was higher than the CG (P<0.05). There was no statistical difference in adverse drug reactions between the two groups (P>0.05).

Conclusion: Compared with α-lipoic acid monotherapy, baicalin capsules combined with α-lipoic acid are effective in treating patients with diabetic peripheral neuropathy, this treatment can obviously relieve the clinical symptoms of patients, improve nerve conduction velocity, reduce oxidative stress and inflammatory injury, and it does not increase adverse reactions. Therefore, it is worthy of promotion.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129320PMC
April 2021

Implantable Peripheral Nerve Stimulation for Trigeminal Neuropathic Pain: A Systematic Review and Meta-Analysis.

Neuromodulation 2021 May 18. Epub 2021 May 18.

Department of Pain, The Third Xiangya Hospital and Institute of Pain Medicine, Central South University, Changsha, China.

Objectives: Implantable peripheral nerve stimulation has been increasingly used to treat neuropathic pain. This neuromodulation strategy may be an alternative option for intractable trigeminal neuropathic pain; however, evidence for this treatment approach remains limited. A systematic review was conducted to identify studies of patients that underwent peripheral nerve stimulation implantation for trigeminal neuropathic pain.

Materials And Methods: Databases including, PubMed, EMBASE, and Cochrane Library were searched up to October 5, 2020. The primary outcomes were changes in pain scores and response rates of neuromodulation therapy. A random effects model was used for meta-analysis. Subgroup analysis was performed to examine the source of heterogeneity.

Results: Thirteen studies including 221 participants were evaluated. The estimated response rate of neuromodulation treatment was 61.3% (95% CI: 44.4-75.9%, I  = 70.733%, p < 0.0001) at the last follow-up. The overall reduction in pain scores was 2.363 (95% CI: 1.408-3.319, I  = 85.723%, p < 0.0001). Subgroup analysis further confirmed that stimulation target (peripheral branch vs. trigeminal ganglion vs. trigeminal nerve root) contributed the heterogeneity across enrolled studies. Better clinical outcome was associated with stimulation of the trigeminal peripheral branch (p < 0.0001).

Conclusion: Peripheral nerve stimulation may be a promising approach in the management of trigeminal neuropathic pain, especially for patients intractable to conventional therapy.
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http://dx.doi.org/10.1111/ner.13421DOI Listing
May 2021

Exploration of irradiation intensity dependent external in-band quantum yield for ZnO and CuO/ZnO photocatalysts.

Phys Chem Chem Phys 2021 May 31;23(18):10768-10779. Epub 2021 Mar 31.

College of Electrical Engineering, Southwest Minzu University, Chengdu 610041, China.

Decorating metal oxides with wide band-gap semiconductor nano-particles constitute an important approach for synthesizing nano-photocatalysts, where the photocatalytic activity is attributed to the band diagram related effective charge separation and external in-band quantum yield (EIQY). However, up to now, the correlation between the irradiation intensity and the functionalization of the in-band quantum yield has not yet been explained. In this work, by investigating the photocatalytic activity of ZnO and CuO/ZnO (CZO) nano-photocatalysts under various irradiative intensities, we show that the effective charge separation in the CuO/ZnO band alignment is sensitive to weak illumination, while ZnO exhibits a competitive photocatalytic activity with CZO under strong illumination. As a consequence, by modifying the irradiation intensity, the intrinsic ZnO can achieve a similar photocatalytic activity to that of metal oxide decorated ZnO. Besides, the optimal photocatalytic activity of CZO is found to be reachable by manipulating the pollutant concentration.
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http://dx.doi.org/10.1039/d0cp06649dDOI Listing
May 2021

Antifouling Peptide Hydrogel Based Electrochemical Biosensors for Highly Sensitive Detection of Cancer Biomarker HER2 in Human Serum.

Anal Chem 2021 05 6;93(19):7355-7361. Epub 2021 May 6.

Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, Shandong Key Laboratory of Biochemical Analysis, Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, People's Republic of China.

A facile strategy for the electrochemical detection of human epidermal growth factor receptor 2 (HER2), a breast cancer biomarker, was presented via the fabrication of an antifouling sensing interface based on the conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) and a biocompatible peptide hydrogel. The peptide hydrogel prepared from a designed short peptide of Phe-Glu-Lys-Phe functionalized with a fluorene methoxycarbonyl group (Fmoc-FEKF) enabled excellent activity preservation for the immobilized biomolecules, and its good hydrophilicity facilitated effective alleviation of nonspecific adsorption or biofouling, while the PEDOT film provided a highly stable and conducting substrate. The developed biosensor was highly sensitive and selective for HER2 detection, with a wide linear response range from 0.1 ng mL to 1.0 μg mL and a low limit of detection of 45 pg mL. Moreover, the peptide hydrogel based biosensor was feasible to use for complex biological samples, and it was capable of detecting HER2 in human serum with clinically acceptable accuracy, manifesting a promising potential for practical application.
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http://dx.doi.org/10.1021/acs.analchem.1c01350DOI Listing
May 2021

High-level production of microbial prodigiosin: A review.

J Basic Microbiol 2021 Jun 5;61(6):506-523. Epub 2021 May 5.

College of Bioengineering, Sichuan University of Science and Engineering, Zigong, China.

Prodigiosin is a natural red pigment derived primarily from secondary metabolites of microorganisms, especially Serratia marcescens. It can also be chemically synthesized. Prodigiosin has been proven to have antitumor, antibacterial, antimalaria, anti-insect, antialgae, and immunosuppressive activities, and is gaining increasing important in the global market because of its great potential application value in clinical medicine development, environmental treatment, preparation of food additives, and so on. Due to the low efficiency of prodigiosin chemical synthesis, high-level prodigiosin of production by microorganisms are necessary for prodigiosin applications. In this paper, the production of prodigiosin by microorganism in recent decades is reviewed. The methods and strategies for increasing the yield of prodigiosin are discussed from the aspects of medium composition, additives, factors affecting production conditions, strain modification, and fermentation methods.
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http://dx.doi.org/10.1002/jobm.202100101DOI Listing
June 2021

Corrigendum to 'Engineering human ventricular heart tissue based on macroporous iron oxide scaffolds' [Acta Biomaterialia 88 (2019) 540-553].

Acta Biomater 2021 Jun 2;127:353. Epub 2021 May 2.

Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Shanghai Key Laboratory of Clinical Geriatric Medicine, Fudan University, Shanghai 200032, China; Shanghai Key Laboratory of Birth Defect, Children's Hospital of Fudan University, Shanghai 201102, China. Electronic address:

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http://dx.doi.org/10.1016/j.actbio.2021.04.031DOI Listing
June 2021

Comprehensive TCM treatments combined with chemotherapy for advanced non-small cell lung cancer: A randomized, controlled trial.

Medicine (Baltimore) 2021 May;100(18):e25690

Oncology Center, The First Affiliated Hospital of Guangzhou University of Chinese Medicine.

Objective: We conducted this study to evaluate the efficacy and safety of traditional Chinese medicine (TCM) in advanced non-small cell lung cancer (NSCLC) patients who underwent chemotherapy.

Design: This was a prospective, open-label, randomized controlled trial. NSCLC patients at stage IIIA, IIIB, or IV were randomly assigned to either TCM plus chemotherapy or chemotherapy alone. The comprehensive TCM treatment consisted of Kang Ai injection, herbal decoction, and Zhenqifuzheng capsules. The primary endpoint was quality of life (QOL) measured by the Functional Assessment of Cancer Therapy-Lung version 4.0. The secondary endpoints were chemotherapy completion rate, tumor response, and adverse events. All assessments were done at baseline, the third week, and the sixth week.

Results: Thirty-nine participants were randomly assigned to the treatment group and 36 to the control group. The QOL scores were significantly improved in the treatment group compared with those of the control group in social well-being (cycle 1, P = .048; cycle 2, P = .015), emotional well-being (cycle 1, P = .047; cycle 2, P = 4.29E-05), and functional well-being (cycle 1, P = .030; cycle 2, P = .003), while the QOL scores in the above 3 domains declined in the control group (P < .05). Both groups had a decline in the physical well-being score (cycle 1, P = .042; cycle 2, P = .017) and lung cancer symptom score (cycle 1, P = .001; cycle 2, P = .001) after 2 courses of intervention. The deterioration in physical well-being and lung cancer symptoms was noticeably smaller in the treatment group (P < .05). There were significant differences between the 2 groups in social well-being, emotional well-being, functional well-being, lung cancer symptom domain, and the total score (P < .05). Patients in the treatment group had a significantly lower incidence of platelet reduction than the control group (P = .028) after 2 cycles of treatment. No significant difference in nonhematological adverse events (AEs) was observed.

Conclusion: This study illustrated that comprehensive TCM treatment could promote the QOL of NSCLC patients, alleviate symptoms, and reduce the AEs caused by chemotherapy, verifying the synergistic and attenuating effects of TCM in NSCLC patients undergoing chemotherapy.

Trial Registration: Chinese Clinical Trial Registry (www.chictr.org.cn): ChiCTR-TRC-13003637.
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http://dx.doi.org/10.1097/MD.0000000000025690DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8104195PMC
May 2021

Tiliroside as a CAXII inhibitor suppresses liver cancer development and modulates E2Fs/Caspase-3 axis.

Sci Rep 2021 Apr 21;11(1):8626. Epub 2021 Apr 21.

Department of Oncology, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, Guangdong, People's Republic of China.

Liver cancer is the fatal cause of cancer deaths worldwide due to its aggressiveness and lack of effective therapies. Tiliroside (CHO) is an active compound extracted from herb plant Tribulus terrestris L., which has been used as alternative therapy in clinic practice. However, its therapeutic use against liver cancer has not been previously reported. Here, we showed that Tiliroside exerted significantly higher anti-proliferation effect on liver cancer cell lines Hep3B and SNU-449 than on liver normal cell THLE-3 cells or NC group, respectively, by using MTS assay. Results from colony formation, immigration and invasion assays support the anticancer efficacy of Tiliroside and its low-toxic property while treating liver normal cell THLE-3. 3D spheroid formation and CD133 expression level also displays its anti-stemness effect. It has been showed that Tiliroside may function as Carbonic anhydrases XII (CAXII) inhibitor and affects apoptotic E2F1/E2F3/Caspase-3 axis by using CAXII esterase activity assay, Human carbonic anhydrase 12 (CA-12) ELISA Kit, quantitative reverse transcription PCR (RT-qPCR) as well as CaspACE Assay System, respectively. In summary, we demonstrate for the first time that Tiliroside suppresses liver cancer development possibly by acting as a novel CAXII inhibitor, which warrant further investigation on its therapeutic implications.
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http://dx.doi.org/10.1038/s41598-021-88133-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8060393PMC
April 2021

Salivary Microbiome Variation in Early Childhood Caries of Children 3-6 Years of Age and Its Association With Iron Deficiency Anemia and Extrinsic Black Stain.

Front Cell Infect Microbiol 2021 23;11:628327. Epub 2021 Mar 23.

Department of Stomatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

ECC is a common clinical manifestation of the oral cavity in childhood and Iron deficiency-anemia (IDA) is a high-risk factor but extrinsic black stain on the tooth surface is a protective factor for caries. There is limited information about oral microecological change in early children who suffer from ECC with IDA and extrinsic black stain (BS). This study enrolled 136 children aged 3-6 years. Dental caries and teeth BS were examined. Saliva was collected for 16S rRNA gene and fingertip blood were for Hemoglobin test. There are 93 ECC including 13 with IDA (IDA ECC) and 80 without IDA (NIDA ECC) and 43 caries free (CF) including 17 with BS (BSCF) and 26 without BS (NBS CF). Statistical analysis of microbiota data showed differences of the oral flora in different groups. The oral flora of the IDA ECC group had a high diversity, while the BSCF group had a low diversity. The bacterial genera Bacillus, Moraxella, and Rhodococcus were enriched in the IDA ECC while Neisseria was enriched in the NIDA ECC. Neisseria only exhibited high abundance in the BSCF, and the remaining genera exhibited high abundance in the NBSCF. Interestingly, the BSCF had the same trend as the NIDA ECC, and the opposite trend was observed with IDA ECC. We established random forest classifier using these biomarkers to predict disease outcomes. The random forest classifier achieved the best accuracy in predicting the outcome of caries, anemia and black stain using seven, one and eight biomarkers, respectively; and the accuracies of the classifiers were 93.35%, 94.62% and 95.23%, respectively. Our selected biomarkers can achieve good prediction, suggesting their potential clinical implications.
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http://dx.doi.org/10.3389/fcimb.2021.628327DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8044945PMC
July 2021

Characterizing sensitivity and coverage of clinical WGS as a diagnostic test for genetic disorders.

BMC Med Genomics 2021 04 13;14(1):102. Epub 2021 Apr 13.

BGI Genomics, BGI-Shenzhen, Shenzhen, 518083, China.

Background: Due to its reduced cost and incomparable advantages, WGS is likely to lead to changes in clinical diagnosis of rare and undiagnosed diseases. However, the sensitivity and breadth of coverage of clinical WGS as a diagnostic test for genetic disorders has not been fully evaluated.

Methods: Here, the performance of WGS in NA12878, the YH cell line, and the Chinese trios were measured by assessing their sensitivity, PPV, depth and breadth of coverage using MGISEQ-2000. We also compared the performance of WES and WGS using NA12878. The sensitivity and PPV were tested using the family-based trio design for the Chinese trios. We further developed a systematic WGS pipeline for the analysis of 8 clinical cases.

Results: In general, the sensitivity and PPV for SNV/indel detection increased with mean depth and reached a plateau at an ~ 40X mean depth using down-sampling samples of NA12878. With a mean depth of 40X, the sensitivity of homozygous and heterozygous SNPs of NA12878 was > 99.25% and > 99.50%, respectively, and the PPV was 99.97% and 98.96%. Homozygous and heterozygous indels showed lower sensitivity and PPV. The sensitivity and PPV were still not 100% even with a mean depth of ~ 150X. We also observed a substantial variation in the sensitivity of CNV detection across different tools, especially in CNVs with a size less than 1 kb. In general, the breadth of coverage for disease-associated genes and CNVs increased with mean depth. The sensitivity and coverage of WGS (~ 40X) was better than WES (~ 120X). Among the Chinese trios with an ~ 40X mean depth, the sensitivity among offspring was > 99.48% and > 96.36% for SNP and indel detection, and the PPVs were 99.86% and 97.93%. All 12 previously validated variants in the 8 clinical cases were successfully detected using our WGS pipeline.

Conclusions: The current standard of a mean depth of 40X may be sufficient for SNV/indel detection and identification of most CNVs. It would be advisable for clinical scientists to determine the range of sensitivity and PPV for different classes of variants for a particular WGS pipeline, which would be useful when interpreting and delivering clinical reports.
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http://dx.doi.org/10.1186/s12920-021-00948-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8045368PMC
April 2021

VPS34 suppression reverses osimertinib resistance via simultaneously inhibiting glycolysis and autophagy.

Carcinogenesis 2021 Jun;42(6):880-890

Department of Respiratory Disease, Daping Hospital, Army Medical University, Chongqing, China.

Autophagy and glycolysis are associated with osimertinib resistance. The energy complement and dynamic balance between these two processes make it difficult to block the process of drug resistance; breaking the complementary relationship between them may effectively overcome drug resistance. However, the exact mechanisms and the key players for regulating autophagy and glycolysis remain unclear. In this study, we demonstrate that autophagy and glycolysis levels in osimertinib-resistant cells were markedly higher than parental cells, and a dynamic balance existed between them. Inhibition of the class III phosphoinositide 3-kinase vacuolar protein sorting 34 (VPS34) with 3-methyladenine or small interfering RNA can not only inhibit abnormally enhanced autophagy but also inhibit glycolysis by inhibiting the location of epidermal growth factor receptor (EGFR) and the expression of hexokinase II. By demonstrating that VPS34 is the key player controlling autophagy and glycolysis simultaneously, our study may provide a new strategy for overcoming osimertinib resistance for treatment of EGFR-mutant non-small cell lung cancer patients.
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http://dx.doi.org/10.1093/carcin/bgab030DOI Listing
June 2021

Archaeal and bacterial community structures of rural household biogas digesters with different raw materials in Qinghai Plateau.

Biotechnol Lett 2021 Jul 3;43(7):1337-1348. Epub 2021 Apr 3.

Qinghai Key Laboratory of Vegetable Genetics and Physiology, Academy of Agriculture and Forestry, Qinghai University, Ningda Road 253, Xining, 810016, Qinghai, China.

The present study aims to investigate microbial community structures household biogas digesters with different raw materials in Qinghai Plateau rural. High-throughput 16S rRNA gene sequencing analysis revealed that Firmicutes, Bacteroidetes, and Proteobacteria are the most abundant bacterial phyla (64.08%). Prevotella group 7 was the most abundant genus in digester YL9 and YL10 (69.72% and 26.96%, respectively) using vegetable waste raw materials. Trichococcus exhibited the highest abundance (14.55%) in YL1 digester using sheep and pig manure. Clostridium sensu stricto 1 (13.89%) and Synergistaceae_uncultured (15.52%) comprised the highest abundances in digester YL5 with mixed raw materials (i.e., dairy manure, sheep manure, and human feces). In addition, Proteiniphilum and Pseudomonas exhibited the highest abundances among bacterial genera in YL4 digester using pig manure. Methanomicrobiales was the most dominant archaeal communities, ranging from 13.35% to 81.34% in abundance. Methanocorpusculum exhibited dominant abundances in all digesters using various raw materials. Methanogenium was the most abundant archaeal genera in YL4 and YL6 digesters, which consume pig manure as primary raw material. In addition, Methanosarcina and Methanosaeta exhibited the highest abundances in digester YL1 (55.03%) and YL9 (51.40%), respectively. Moreover, fermentation temperatures and pH both contributed to the archaeal and bacterial community structures in all the investigated digesters. Specially, fermentation temperature showed positive correlation with the abundances of Synergistaceae_uncultured, Methanogenium, and Methanosaeta, and pH was positively correlated with the abundances of Prevotella group 7 and Methanosarcina abundances.
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http://dx.doi.org/10.1007/s10529-021-03105-1DOI Listing
July 2021

Combined Transcriptome Analysis Reveals the Ovule Abortion Regulatory Mechanisms in the Female Sterile Line of Carr.

Int J Mol Sci 2021 Mar 19;22(6). Epub 2021 Mar 19.

College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.

Ovule abortion is a common phenomenon in plants that has an impact on seed production. Previous studies of ovule and female gametophyte (FG) development have mainly focused on angiosperms, especially in . However, because it is difficult to acquire information about ovule development in gymnosperms, this remains unclear. Here, we investigated the transcriptomic data of natural ovule abortion mutants (female sterile line, STE) and the wild type (female fertile line, FER) of Carr. to evaluate the mechanism of ovule abortion during the process of free nuclear mitosis (FNM). Using single-molecule real-time (SMRT) sequencing and next-generation sequencing (NGS), 18 cDNA libraries via Illumina and two normalized libraries via PacBio, with a total of almost 400,000 reads, were obtained. Our analysis showed that the numbers of isoforms and alternative splicing (AS) patterns were significantly variable between FER and STE. The functional annotation results demonstrate that genes involved in the auxin response, energy metabolism, signal transduction, cell division, and stress response were differentially expressed in different lines. In particular, , , , and had significantly lower expression in STE, showing that auxin might be insufficient in STE, thus hindering nuclear division and influencing metabolism. Apoptosis in STE might also have affected the expression levels of these genes. To confirm the transcriptomic analysis results, nine pairs were confirmed by quantitative real-time PCR. Taken together, these results provide new insights into ovule abortion in gymnosperms and further reveal the regulatory mechanisms of ovule development.
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http://dx.doi.org/10.3390/ijms22063138DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8003466PMC
March 2021

Serial Quantitative Chest CT Assessment of COVID-19: A Deep Learning Approach.

Radiol Cardiothorac Imaging 2020 Apr 30;2(2):e200075. Epub 2020 Mar 30.

Department of Radiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Jiefang Avenue 1095, 430030 Wuhan, China (L.H., T.A., L.X.); Department of Radiology, Wuhan No. 1 Hospital, Wuhan, China (R.H.); Institute of Advanced Research, Infervison, Beijing, China (P.Y., H.K.); and Division of Imaging Processing, Department of Radiology, Leiden University Medical Center, Leiden, the Netherlands (Q.T.).

Purpose: To quantitatively evaluate lung burden changes in patients with coronavirus disease 2019 (COVID-19) by using serial CT scan by an automated deep learning method.

Materials And Methods: Patients with COVID-19, who underwent chest CT between January 1 and February 3, 2020, were retrospectively evaluated. The patients were divided into mild, moderate, severe, and critical types, according to their baseline clinical, laboratory, and CT findings. CT lung opacification percentages of the whole lung and five lobes were automatically quantified by a commercial deep learning software and compared with those at follow-up CT scans. Longitudinal changes of the CT quantitative parameter were also compared among the four clinical types.

Results: A total of 126 patients with COVID-19 (mean age, 52 years ± 15 [standard deviation]; 53.2% males) were evaluated, including six mild, 94 moderate, 20 severe, and six critical cases. CT-derived opacification percentage was significantly different among clinical groups at baseline, gradually progressing from mild to critical type (all < .01). Overall, the whole-lung opacification percentage significantly increased from baseline CT to first follow-up CT (median [interquartile range]: 3.6% [0.5%, 12.1%] vs 8.7% [2.7%, 21.2%]; < .01). No significant progression of the opacification percentages was noted from the first follow-up to second follow-up CT (8.7% [2.7%, 21.2%] vs 6.0% [1.9%, 24.3%]; = .655).

Conclusion: The quantification of lung opacification in COVID-19 measured at chest CT by using a commercially available deep learning-based tool was significantly different among groups with different clinical severity. This approach could potentially eliminate the subjectivity in the initial assessment and follow-up of pulmonary findings in COVID-19.© RSNA, 2020.
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http://dx.doi.org/10.1148/ryct.2020200075DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7233442PMC
April 2020

Universally Optimal Verification of Entangled States with Nondemolition Measurements.

Phys Rev Lett 2021 Mar;126(9):090504

Key Laboratory of Advanced Optoelectronic Quantum Architecture and Measurement of Ministry of Education, School of Physics, Beijing Institute of Technology, Beijing 100081, China.

The efficient and reliable characterization of quantum states plays a vital role in most, if not all, quantum information processing tasks. In this work, we present a universally optimal protocol for verifying entangled states by employing the so-called quantum nondemolition measurements, such that the verification efficiency is equivalent to that of the optimal global strategy. Instead of being probabilistic as the standard verification strategies, our protocol is constructed sequentially, which is thus more favorable for experimental realizations. In addition, the target states are preserved in the protocol after each measurement, so can be reused in any subsequent tasks. We demonstrate the power of our protocol for the optimal verification of Bell states, arbitrary two-qubit pure states, and stabilizer states. We also prove that our protocol is able to perform tasks including fidelity estimation and state preparation.
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http://dx.doi.org/10.1103/PhysRevLett.126.090504DOI Listing
March 2021

Defective Ultrafine MnO Nanoparticles Confined within a Carbon Matrix for Low-Temperature Oxidation of Volatile Organic Compounds.

Environ Sci Technol 2021 04 22;55(8):5403-5411. Epub 2021 Mar 22.

Tianjin Key Laboratory of Indoor Air Environmental Quality Control, School of Environmental Science and Technology, Tianjin University, Tianjin 300350, China.

The development of catalysts for volatile organic compound (VOC) treatment by catalytic oxidation is of great significance to improve the atmospheric environment. Size-effect and oxygen vacancy engineering are effective strategies for designing high-efficiency heterogeneous catalysts. Herein, we explored the in situ carbon-confinement-oxidation method to synthesize ultrafine MnO nanoparticles with adequately exposed defects. They exhibited an outstanding catalytic performance with a of 167 °C for acetone oxidation, which is 73 °C lower than that of bulk MnO (240 °C). This excellent catalytic activity was primarily ascribed to their high surface area, rich oxygen vacancies, abundant active oxygen species, and good reducibility at low temperatures. Importantly, the synthesized ultrafine MnO exhibited impressive stability in long-term, cycling and water-resistance tests. Moreover, the possible mechanism for acetone oxidation over MnO-NA was revealed. In this work, we not only prepared a promising material for removing VOCs but also provided a new strategy for the rational design of ultrafine nanoparticles with abundant defects.
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http://dx.doi.org/10.1021/acs.est.0c08335DOI Listing
April 2021

Biocompatible peptide hydrogels with excellent antibacterial and catalytic properties for electrochemical sensing application.

Anal Chim Acta 2021 Apr 10;1154:338295. Epub 2021 Feb 10.

Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, Shandong Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China. Electronic address:

A unique electrochemical sensor was constructed based on designed peptide hydrogels loaded with ciprofloxacin and gold nanoparticles, which exhibited excellent biocompatibility, antibacterial capability and electrochemical catalytic property. The peptide hydrogel was prepared base on the self-assembly of a designed short peptide sequence of Phe-Glu-Lys-Phe (FEKF) with the N-terminal modified with a fluorene methoxycarbonyl (Fmoc) group. The peptide hydrogel possessed nanofibrous network structure and exhibited good shear-thinning behavior and excellent biocompatibility, and it can be easily doped with gold nanoparticles and the antibiotic drug ciprofloxacin. The loaded antibacterial drug offered remarkable antibacterial activity of the hydrogel, while the loaded gold nanoparticles rendered the hydrogel excellent electrochemical catalytic capability towards the detection of a typical neurotransmitter dopamine. The combination of the antibacterial property and the electrochemical catalytic ability within a peptide hydrogel ensured the development of sensitive and antibacterial electrochemical sensors, and this strategy was expected to promote the construction of implantable sensors without infection.
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http://dx.doi.org/10.1016/j.aca.2021.338295DOI Listing
April 2021

Bovine Serum Albumin-Cross-Linked Polyaniline Nanowires for Ultralow Fouling and Highly Sensitive Electrochemical Protein Quantification in Human Serum Samples.

Anal Chem 2021 03 26;93(9):4326-4333. Epub 2021 Feb 26.

Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, Shandong Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.

Biofouling represents a serious challenge for the assaying of disease markers with various biosensors in complex biological samples due to the accompanied nonspecific protein adsorption. Herein, a highly sensitive and antifouling biosensing interface was constructed based on a cost-effective inert protein bovine serum albumin (BSA) cross-linked with polyaniline nanowires (PANI-NWs). Compared with the physically adsorbed BSA that was commonly used to block nonspecific adsorption or binding of proteins, the cross-linked BSA exhibited a significantly enhanced antifouling capability. The BSA/PANI-NW-modified electrode interface possessed excellent antifouling capability and electrochemical activity owing to the presence of the cross-linked BSA and the conducting polymer polyaniline. With further immobilization of the peptide aptamer for immunoglobulin G (IgG) recognition onto the BSA/PANI-NW interface, an electrochemical biosensor with excellent selectivity and sensitivity was prepared. The IgG biosensor possessed a linear range from 1.0 ng mL to 10 μg mL and a low detection limit of 0.27 ng mL, and it was capable of assaying IgG in complex human serum samples with acceptable accuracy when compared with the assay results obtained using commercial enzyme-linked immunosorbent assay kits. It is expected that the unique BSA-cross-linked conducting polymers can be used for the construction of various electrochemical sensors and biosensors that can be applied in complex biological media.
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http://dx.doi.org/10.1021/acs.analchem.1c00089DOI Listing
March 2021

Analysis of Molecular Mechanism of YiqiChutan Formula Regulating DLL4-Notch Signaling to Inhibit Angiogenesis in Lung Cancer.

Biomed Res Int 2021 12;2021:8875503. Epub 2021 Feb 12.

Department of Medical Technologic, The First Affiliated Hospital to Guangzhou University of Chinese Medicine, Guangzhou 510405, China.

In order to explore the specific mechanism of YiqiChutan formula (YQCTF) in inhibiting the angiogenesis of lung cancer and its relationship with delta-like ligand 4- (DLL4-) Notch signaling, 30 healthy BALB/c-nu/nu rats were selected and divided into three groups: A549 group (implanted with lung adenocarcinoma cell line A549), NCI-H460 group (implanted with human lung large-cell carcinoma cell line NCI-H460), and NCI-H446 group (implanted with human lung small cell carcinoma cell line NCI-H446) for constructing lung cancer transplanted tumor models. After modeling, the group treated with normal saline was taken as control group, 200 mg/kg of YQCTF was adopted for intervention, and the tumor volume and growth inhibition rate were compared with the vascular targeted inhibitor Sorafenib. HE staining, CD31 fluorescent antibody staining, and microelectron microscopy were adopted to observe the neovascular endothelial cells of the transplanted tumor. The expression of VEGF, HIF-1, DLL4, and Notch-1 in the transplanted tumors in each group was detected by Western blot and RT-PCR at the protein level or mRNA level. Compared with the control group, the YQCTF-treated group had obvious inhibitory effect on lung cancer transplanted tumor and lung cancer angiogenesis. In the YQCTF-treated group, the density of angiogenesis decreased significantly and the vascular lumen structure also decreased, and the expression levels of VEGF, HIF-1, DLL4, and Notch-1 in the YQCTF-treated group were all lower than those in the control group. YQCTF could inhibit the growth of lung cancer transplanted tumor through antiangiogenesis, and it could also reduce the amount of angiogenesis in lung cancer transplanted tumor. In addition, the generation of lumen structure was also hindered, which was realized through the VEGF signaling pathway and DLL4-Notch signaling pathway.
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http://dx.doi.org/10.1155/2021/8875503DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7895574PMC
May 2021

HPV58 E7 Protein Expression Profile in Cervical Cancer and CIN with Immunohistochemistry.

J Cancer 2021 18;12(6):1722-1728. Epub 2021 Jan 18.

Department of Dermatology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China.

The persistent infection of high-risk human papillomavirus (HR-HPV) is one of the most common causes of cervical cancer worldwide, and HPV type 58 (HPV58) is the third most common HPV type in eastern Asia. The E7 oncoprotein is constitutively expressed in HPV58-associated cervical cancer cells and plays a key role during tumorigenesis. This study aimed to assess the HPV58 E7 protein expression in the tissues of cervical cancer and cervical intraepithelial neoplasia (CIN). A total of 67 HPV58-positive cervical samples were collected, including 25 cervical cancer samples and 42 CIN samples. All the tissues were examined by HPV58 E7, p16 and Ki67 immunohistochemistry (IHC). At last, we analyzed their association with clinical and pathological variables. HPV58 E7 expression was detected in 96% of the HPV58 DNA-positive cervical cancer tissues and 85.7% of HPV58-positive CIN tissues. 65 samples of cervical cancer and CIN tissues had p16-positive staining, while 59 samples were Ki-67 positive. HPV58 E7 protein is highly expressed in both cervical cancer and CIN tissues. HPV58 E7 IHC could be sensitive and specific for evaluating HPV-driven cervical cancer and pre-cancerous lesions, in combination with p16 and Ki-67 IHC.
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http://dx.doi.org/10.7150/jca.50816DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890325PMC
January 2021
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