Publications by authors named "Rui Du"

208 Publications

Prevalence of Bovine Tuberculosis in Yaks Between 1982 and 2020 in Mainland China: A Systematic Review and Meta-Analysis.

Vector Borne Zoonotic Dis 2021 Mar 1. Epub 2021 Mar 1.

College of Chinese Medicine Materials, Jilin Agricultural University, Changchun, China.

Bovine tuberculosis (bTB) is a major chronic bacterial disease in cattle and is the major economic and animal welfare issue in the world. Although the economic costs and public health safety risks associated with the disease are considerable, the overall epidemiology of the Chinese yak () bTB is unclear. To fully reveal the basic prevalence of yak bTB in different regions of China, we searched five databases including PubMed, Science Direct, CNKI (China National Knowledge Infrastructure), Wanfang and Chongqing VIP. Based on the incidence and prevalence of yak tuberculosis in China from 1982 to 2020, a meta-analysis of yak bTB in China was established for the first time. By formulating the search formula, 97 studies were searched in five databases. According to the established exclusion criteria and excluded comments and repeated and irrelevance research, we finally selected 19 cross-sectional studies, which showed the prevalence of bTB in Chinese yaks. Random-effect meta-regression model analysis showed that the estimated prevalence of 122,729 yaks in China was 1.0 (95% confidence interval [CI]: 0.0-1.0). The regional prevalence varies greatly, northwest China prevalence rate 0. 39% (95% CI: 0.2-0. 64) and southwest China prevalence rate 2.59% (95% CI: 1.94-3.34); in terms of province level, the prevalence was highest in Tibet 2.59% (95% CI: 1.94-3.34), followed by Xinjiang 2.36% (95% CI: 0.86-4.58), and Shanxi has the lowest 0.00% (95% CI: 0.00-0.98). This systematic review and meta-analysis identified the estimated prevalence of bTB in Chinese yaks and estimated the underlying factors associated with bTB, including geographic location, sampling year, age, and TB detection method. Provide evidence to plan corresponding disease control strategies for policymakers and to assess future economic risks accurately.
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http://dx.doi.org/10.1089/vbz.2020.2687DOI Listing
March 2021

Seroprevalence and Risk Factors of the Bluetongue Virus in Cattle in China From 1988 to 2019: A Comprehensive Literature Review and Meta-Analysis.

Front Vet Sci 2020 28;7:550381. Epub 2021 Jan 28.

College of Chinese Medicine Materials, Jilin Agricultural University, Changchun, China.

Bluetongue caused by the bluetongue virus (BTV) is a non-contagious and an insect-borne disease mainly affecting domestic and wild ruminants. Bluetongue in cattle is associated with vesicular lesions, weight loss, low milk production, and low reproductive capacity. It should not be ignored as it is associated with large economic losses to the livestock breeding industry in China. Although many studies have investigated bluetongue virus infection in cattle, no nationwide study on the prevalence of bluetongue virus infection in cattle from China has yet been conducted. This meta-analysis aimed to evaluate the seroprevalence and risk factors for bluetongue in cattle. We collected 50 publications from 1988 to 2019 through PubMed, ScienceDirect, Chinese Web of Knowledge (CNKI), VIP Chinese journal database, and Wanfang database. A total of the pooled bluetongue seroprevalence of 12.2% (5,332/87,472) in cattle was tested. The point estimate of bluetongue collected from 2001 to 2011 was 22.5% (95% CI: 1.2-58.9), which was higher than after 2012 (9.9%, 95% CI: 3.3-19.4). The analysis of the feeding model subgroup revealed that the seroprevalence of bluetongue was significantly higher ( < 0.05) among free-range cattle (22.5%; 95% CI: 7.7-42.3) than among cattle from intensive farming systems (1.8%; 95% CI: 0.0-6.7). The seroprevalence of bluetongue in different species showed significant variation ( < 0.05), with the highest seroprevalence of 39.8% (95% CI: 18.7-63.0) in buffalo and the lowest seroprevalence of 4.3% (95% CI: 1.2-9.0) in yak. In the zoogeographical division subgroup, the seroprevalence of bluetongue correlated positively within a certain range with the species distribution of . Our findings suggested that bluetongue was prevalent in cattle in China. In addition, the contact with sheep, other ruminants, or transmission media such as may increase the seroprevalence of bluetongue disease in cattle. It is necessary to carry out continuous monitoring of the bluetongue seroprevalence. Moreover, comprehensive and improved strategies and measures should be implemented to prevent and control the spread of bluetongue.
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http://dx.doi.org/10.3389/fvets.2020.550381DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7901971PMC
January 2021

Postoperative morbidity and mortality after anterior resection with preventive diverting loop ileostomy versus loop colostomy for rectal cancer: A updated systematic review and meta-analysis.

Eur J Surg Oncol 2021 Feb 18. Epub 2021 Feb 18.

Clinical Medical College of Yangzhou University, General Surgery Institute of Yangzhou-Yangzhou University, Northern Jiangsu People's Hospital, Nantong Road No.98, Yangzhou 225001, China. Electronic address:

The purpose of this meta-analysis was to evaluate the perioperative morbidity after anterior resection with diverting loop ileostomy (LI) versus colostomy (LC) and its reversal for rectal cancer. The studies on the application of loop ileostomy versus loop colostomy in anterior resection published from January 2000 to January 2020 were searched in the databases of Pubmed, Embase, Cochrane library, and Clinical trials. All randomized controlled trials (RCTs) and cohort studies were included according to inclusion criteria. Eight studies (2 RCTs and 6 cohort studies) totaling 1451 patients (821 LI and 630 LC) were included in the meta-analysis. The morbidity related to stoma formation and closure did not demonstrate significant differences. Significantly more LCs were complicated by stoma prolapse & retraction (OR:0.26,95%CI:0.11-0.60,P = 0.001), parastomal hernia (OR = 0.52,95%CI:0.30-0.88, P = 0.01), surgical site infection (SSI) (OR = 0.24,95%CI:0.11-0.49,P < 0.0001) and incisional hernias (OR = 0.39,95%CI:0.19-0.83,P = 0.01) than by LIs. Patients with LI demonstrated significantly more complications related to the stoma, such as dehydration (OR = 0.52,95%CI:0.30-0.88, P = 0.01) and ileus (OR = 2.23,95%CI:1.12-4.43, P = 0.02) than patients with LC. While after the subgroup analysis of different publication years, LI could reduce the risk of the morbidity after stoma formation in previous years group (P = 0.04) with a lower heterogeneity (I = 37%); LC could reduce the incidence of parastomal dermatitis in recent years group (P < 0.0001) without heterogeneity in each subgroup (I = 0%). Cumulative meta-analysis detected significant turning points in dehydration, SSI, and ileus. This meta-analysis recommends diverting LI in the anterior resection for rectal cancer, but there is a risk of dehydration, irritant dermatitis, and ileus.
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http://dx.doi.org/10.1016/j.ejso.2021.01.030DOI Listing
February 2021

Protective effects of 3,4-seco-lupane triterpenes from food raw materials of the leaves of Eleutherococcus senticosus and Eleutherococcus sessiliflorus on arrhythmia induced by barium chloride.

Chem Biodivers 2021 Feb 21. Epub 2021 Feb 21.

Jilin Agricultural University, College of Chinese MEdicinal, Xincheng Street 2888, 130118, Changchun, CHINA.

As a traditional wild vegetable and food raw material, the leaves of Eleutherococcus senticosus and Eleutherococcus sessiliflorus are rich in 3,4-seco-lupane triterpenes, including chiisanoside (CSS), divaroside (DVS), sessiloside-A (SSA), and chiisanogenin (CSG). This study was to evaluate the antiarrhythmic effects of these 3,4-seco-lupane triterpenes. Cell viability and apoptosis were measured by the CCK-8 method. In vivo, arrhythmia was induced by rapid injection of BaCl 2 via rat caudal vein. The occurrence time and duration of arrhythmias in rats were observed. The levels of SOD and MDA in serum, and Na + -K + -ATPase and Ca 2+ -Mg 2+ -ATPase in myocardial homogenate were detected by ELISA, and the histopathological changes were observed by HE staining. Protein expression were studied by Western blot. Analysis of 3,4-seco-lupane triterpenes interactions with protein kinase A by molecular docking. In the present study, we found that 3,4-seco-lupane triterpenes exhibited powerful antiarrhythmic activity, especially DVS completely relieved the ventricular arrhythmia induced by BaCl 2 . This study suggests that the leaves of E. senticosus and E. sessiliflorus might be used as functional food materials to prevent arrhythmia, and DVS was expected to be developed as an antiarrhythmic drug.
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http://dx.doi.org/10.1002/cbdv.202001021DOI Listing
February 2021

Spinophilin modulates pain through suppressing dendritic spine morphogenesis via negative control of Rac1-ERK signaling in rat spinal dorsal horn.

Neurobiol Dis 2021 Feb 17;152:105302. Epub 2021 Feb 17.

Institute for Biomedical Sciences of Pain, Tangdu Hospital, The Fourth Military Medical University, Xi'an 710038, Shaanxi Province, PR China; Key Laboratory of Brain Stress and Behavior, People's Liberation Army, Xi'an 710038, PR China. Electronic address:

Both spinophilin (SPN, also known as neurabin 2) and Rac1 (a member of Rho GTPase family) are believed to play key roles in dendritic spine (DS) remodeling and spinal nociception. However, how SPN interacts with Rac1 in the above process is unknown. Here, we first demonstrated natural existence of SPN-protein phosphatase 1-Rac1 complex in the spinal dorsal horn (DH) neurons by both double immunofluorescent labeling and co-immunoprecipitation, then the effects of SPN over-expression and down-regulation on mechanical and thermal pain sensitivity, GTP-bound Rac1-ERK signaling activity, and spinal DS density were studied. Over-expression of SPN in spinal neurons by intra-DH pAAV-CMV-SPN-3FLAG could block both mechanical and thermal pain hypersensitivity induced by intraplantar bee venom injection, however it had no effect on the basal pain sensitivity. Over-expression of SPN also resulted in a significant decrease in GTP-Rac1-ERK activities, relative to naive and irrelevant control (pAAV-MCS). In sharp contrast, knockdown of SPN in spinal neurons by intra-DH pAAV-CAG-eGFP-U6-shRNA[SPN] produced both pain hypersensitivity and dramatic elevation of GTP-Rac1-ERK activities, relative to naive and irrelevant control (pAAV-shRNA [NC]). Moreover, knockdown of SPN resulted in increase in DS density while over-expression of it had no such effect. Collectively, SPN is likely to serve as a regulator of Rac1 signaling to suppress DS morphogenesis via negative control of GTP-bound Rac1-ERK activities at postsynaptic component in rat DH neurons wherein both mechanical and thermal pain sensitivity are controlled.
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http://dx.doi.org/10.1016/j.nbd.2021.105302DOI Listing
February 2021

ICT1 Promotes Osteosarcoma Cell Proliferation and Inhibits Apoptosis via STAT3/BCL-2 Pathway.

Biomed Res Int 2021 22;2021:8971728. Epub 2021 Jan 22.

Department of Orthopedics, The Affiliated Hospital of Nanjing Medical University, Changzhou No.2 People's Hospital, Changzhou, Jiangsu 213003, China.

Osteosarcoma (OS) is a familiar malignant bone tumor that occurs mainly in adolescents. Immature colon carcinoma transcript-1 (ICT1) is an important member of the large mitoribosomal subunit in mitochondrial ribosomes, which has been shown to be closely related to tumorigenesis. Its expression and function in OS, however, remained unclear. Here, we showed that ICT1 was significantly upregulated in OS and promoted the growth of OS cells. Mechanistically, ICT1 acted as an oncogene in OS and promoted proliferation and inhibited apoptosis of OS cells through the STAT3/BCL-2 axis. These results reveal a novel insight into the role of the ICT1/STAT3/BCL-2 axis in OS and therefore may represent a novel molecular target for novel treatments.
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http://dx.doi.org/10.1155/2021/8971728DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7853870PMC
January 2021

The Application of Enhanced Recovery After Surgery (ERAS) for Patients Undergoing Bariatric Surgery: a Systematic Review and Meta-analysis.

Obes Surg 2021 Mar 9;31(3):1321-1331. Epub 2021 Jan 9.

Department of General Surgery, Northern Jiangsu People's Hospital, Nantong Road No. 98, Yangzhou, 225001, China.

To systematically evaluate the effectiveness and safety of the enhanced recovery after surgery (ERAS) pathway in bariatric surgery. A literature search was conducted using PubMed, Medline, EMBASE, OVID, World Health Organization International Trial Register, and Cochrane Library identifying all eligible studies comparing ERAS protocols with standard care (SC) in bariatric surgery through May 2020. Relevant perioperative parameters were extracted from the resulting studies for meta-analysis. The primary outcome was the length of hospital stay, and secondary outcomes included operation time, postoperative nausea, and vomiting (PONV), postoperative complications, readmission, reoperation, and subsequent emergency room visits. Postoperative complications were categorized according to the Clavien-Dindo classification. Final analysis included five randomized controlled trials (RCTs) and twelve observational studies which included 4964 patients in the ERAS group and 3218 patients in the SC group. The length of the hospital stay was significantly decreased (p < 0.01) after ERAS protocol management, as did the incidence of POVN (p < 0.01). No significant differences were observed between the ERAS group and SC group in terms of operation time (p = 0.37), postoperative complications (p = 0.18), readmission (p = 0.17), reoperation (p = 0.34), or emergency room visits (p = 0.65). The application of ERAS protocols in bariatric surgery is safe and feasible, effectively shortening the length of a hospital stay without compromising morbidity, and accelerating patient recovery.
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http://dx.doi.org/10.1007/s11695-020-05209-5DOI Listing
March 2021

Association of Serum Bile Acids Profile and Pathway Dysregulation With the Risk of Developing Diabetes Among Normoglycemic Chinese Adults: Findings From the 4C Study.

Diabetes Care 2021 Feb 18;44(2):499-510. Epub 2020 Dec 18.

Department of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Objective: Comprehensive assessment of serum bile acids (BAs) aberrations before diabetes onset remains inconclusive. We examined the association of serum BA profile and coregulation with the risk of developing type 2 diabetes mellitus (T2DM) among normoglycemic Chinese adults.

Research Design And Methods: We tested 23 serum BA species in subjects with incident diabetes ( = 1,707) and control subjects ( = 1,707) matched by propensity score (including age, sex, BMI, and fasting glucose) from the China Cardiometabolic Disease and Cancer Cohort (4C) Study, which was composed of 54,807 normoglycemic Chinese adults with a median follow-up of 3.03 years. Multivariable-adjusted odds ratios (ORs) for associations of BAs with T2DM were estimated using conditional logistic regression.

Results: In multivariable-adjusted logistic regression analysis, per SD increment of unconjugated primary and secondary BAs were inversely associated with incident diabetes, with an OR (95% CI) of 0.89 (0.83-0.96) for cholic acid, 0.90 (0.84-0.97) for chenodeoxycholic acid, and 0.90 (0.83-0.96) for deoxycholic acid ( < 0.05 and false discovery rate <0.05). On the other hand, conjugated primary BAs (glycocholic acid, taurocholic acid, glycochenodeoxycholic acid, taurochenodeoxycholic acid, and sulfated glycochenodeoxycholic acid) and secondary BA (tauroursodeoxycholic acid) were positively related with incident diabetes, with ORs ranging from 1.11 to 1.19 (95% CIs ranging between 1.05 and 1.28). In a fully adjusted model additionally adjusted for liver enzymes, HDL cholesterol, diet, 2-h postload glucose, HOMA-insulin resistance, and waist circumference, the risk estimates were similar. Differential correlation network analysis revealed that perturbations in intraclass (i.e., primary and secondary) and interclass (i.e., unconjugated and conjugated) BA coregulation preexisted before diabetes onset.

Conclusions: These findings reveal novel changes in BAs exist before incident T2DM and support a potential role of BA metabolism in the pathogenesis of diabetes.
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http://dx.doi.org/10.2337/dc20-0884DOI Listing
February 2021

Enhanced simultaneous nitrogen and phosphorus removal from low COD/TIN domestic wastewater through nitritation-denitritation coupling improved anammox process with an optimal Anaerobic/Oxic/Anoxic strategy.

Bioresour Technol 2021 Feb 10;322:124526. Epub 2020 Dec 10.

National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China. Electronic address:

Advanced nitrogen and phosphorus removal in a single-stage suspending-sludge system was achieved by employing a novel Anaerobic/Oxic/Anoxic (AOA) strategy over 200 days. Satisfactory total inorganic nitrogen (TIN) removal efficiency of 90.4% was achieved and effluent phosphorus was below 0.5 mg/L when treating domestic wastewater with the chemical oxygen demand (COD)/TIN as low as 2.98 ± 1.26. Stable nitritation was maintained with the ammonia residual and low dissolved oxygen of 0.2-0.5 mg/L at aerobic stage following by a post anoxic stage. The much higher activity of ammonia oxidation bacteria (12.99 mgN/gVSS/h) was achieved than the nitrite oxidation bacteria (0.09 mgN/gVSS/h). Notably, improved anammox performance was obtained without initial inoculation, contributing 47.4% to TIN removal. The abundance of Nitrosomonas increased from 0.12% to 0.95% (P < 0.001) and self-enrichment of anammox bacteria Ca. Brocadia was confirmed. It provided new insight into the advanced nutrient removal with comprehensible regulation and less aeration requirement.
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http://dx.doi.org/10.1016/j.biortech.2020.124526DOI Listing
February 2021

Prevalence of bovine leukemia in 1983-2019 in China: A systematic review and meta-analysis.

Microb Pathog 2021 Jan 6;150:104681. Epub 2020 Dec 6.

Laboratory of Production and Product Application of Sika Deer of Jilin Province, Jilin Agricultural University, Changchun, Jilin Province 130118, PR China. Electronic address:

Bovine leukemia is a chronic, progressive, contagious tumor disease characterized by malignant lymphoid cell hyperplasia and systemic lymphadenopathy, and is caused by bovine leukemia virus (BLV). The disease affects almost all countries and regions where livestock are raised, and may even be a potential zoonotic disease. Monitoring and early prevention of bovine leukemia is very important. Therefore, we conducted this meta-analysis, the first of its type in the country, to estimate the prevalence of bovine leukemia in 1983-2019 in China. We included a total of 35 publications reported in 1983-2019 from the PubMed, ScienceDirect, Chinese Web of Knowledge (CNKI), VIP Chinese, and Wan Fang databases. In those articles, a total of 34,954 cattle had been tested, of which 4701 were positive for BLV infection. The estimated pooled BLV prevalence was 10.0% (4701/34,954). Subgroup analysis showed that there were significant differences for sampling years, detection methods, and age. BLV prevalence was highest in the following subgroups: sampled before 1985 (38.5%, 437/1134), age 3-5 years (22.5%, 231/1044), and detected by PCR (17.9%, 1228/5100). Regarding geographic factors, there were significant differences in the latitude and elevation subgroups. BLV prevalence was lowest in the subgroups of 20-30° latitude (3.3%, 255/5069) 200-1000 m altitude (2.2%, 560/11,990). We also analyzed other subgroups such as region, variety, breeding method, precipitation, humidity, and temperature, however, the differences were not significant. Our research indicated that the BLV was still prevalent in some of areas in China. We recommend strengthening the testing of cattle aged >1 year and using flexible testing methods such as PCR to control the prevalence of bovine leukemia and to prevent persistent infection.
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http://dx.doi.org/10.1016/j.micpath.2020.104681DOI Listing
January 2021

Survey of background microbial index in inhalable particles in Beijing.

Sci Total Environ 2021 Feb 24;757:143743. Epub 2020 Nov 24.

College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China.

As a potential transmission route for diseases, aerosols have an important impact on human health. At present, research concerning the biological components of atmospheric particulate matter (PM) is of increasing interest. However, previous research has mainly focused on serious pollution conditions, creating a knowledge gap regarding background atmospheric microbes. In this study, we observed the atmosphere of Huairou in Beijing for one year, analyzed the characteristics of the physiological metabolic activity of the microorganisms as an index to determine the air quality, and further explored the microbial communities. From January 2018 to January 2019, a total of 157 days of microbial activity data for PM and PM were obtained through the use of a modified fluorescein diacetate (FDA) hydrolysis method. Our results showed that there was no significant difference between the microbial activity of PM and PM, even though there was significant seasonal variation. At increasing pollution levels, the results showed that the microbial activity decreased at first, and then increased as the conditions worsened. The microbial community of PM was analyzed using the high-throughput sequencing method. There were significant seasonal differences in species richness and community diversity of bacteria in PM, whereas there was variation only in its fungi species richness. Notably, the microbial community dominated by bacteria has a significant influence on microbial activity. From the perspective of microbial community composition, this study uncovered the possible causes of microbial activity variation and identified the key bacteria and fungi. These results will provide a theoretical basis for both improving air biological pollution predictions and ambient air quality evaluations.
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http://dx.doi.org/10.1016/j.scitotenv.2020.143743DOI Listing
February 2021

Age at menarche, ideal cardiovascular health metrics, and risk of diabetes in adulthood: Findings from the REACTION study.

J Diabetes 2020 Nov 1. Epub 2020 Nov 1.

Department of Endocrine and Metabolic Diseases, Shanghai Institute of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: Age at menarche was reported to be associated with the risk of diabetes. However, the impact of ideal cardiovascular health metrics (ICVHMs) on the association between age at menarche and adulthood diabetes risk was unclear.

Methods: We included 121 431 women from the nationwide, population-based cohort of the REACTION study (Risk Evaluation of Cancers in Chinese Diabetic Individuals: a Longitudinal Study). The diagnosis of diabetes was based on the oral glucose tolerance test (OGTT) and glycosylated hemoglobin (HbA1c) measurement. Logistic regression and multiplicative interaction analysis were conducted to investigate the potential interaction effect between age at menarche and ICVHMs on the development of diabetes.

Results: The multivariable-adjusted odds ratios of diabetes across categories of age at menarche (<14, 14-17, and > 17 years) were 1.22 (95% confidence interval [CI]: 1.17, 1.28), 1.00 (reference), and 0.89 (95% CI: 0.85, 0.93), respectively. In subgroup analysis, significant interactions were detected between total cholesterol/blood pressure levels and age at menarche regarding the risk of diabetes (P for interaction = .0091 and .0019, respectively). The increased risk associated with age at menarche <14 years was observed in participants with three or fewer ICVHMs, but not in women with four or more ICVHMs (P for interaction = .0001).

Conclusions: Age at menarche was inversely associated with the risk of diabetes in adulthood in Chinese women, and it appeared to be modified by the presence of ICVHMs. Further studies are needed to clarify the precise interrelationship and the generalizability of our results.
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http://dx.doi.org/10.1111/1753-0407.13128DOI Listing
November 2020

Whether stoma support rods have application value in loop enterostomy: a systematic review and meta-analysis.

World J Surg Oncol 2020 Oct 22;18(1):269. Epub 2020 Oct 22.

Department of General Surgery, Northern Jiangsu People's Hospital, Nantong Road No.98, Yangzhou, 225001, China.

Purpose: The purpose of the systematic review and meta-analysis is to analyze the application value of the stoma support rods in loop enterostomy.

Methods: The studies on the application of stoma rods in loop enterostomy published from January 2000 to January 2020 were searched in the databases of PubMed, Embase, Cochrane library, and Clinical trials. All randomized controlled trials (RCTs) and cohort studies that observed the value of stoma rods were included according to inclusion criteria. The RevMan5.3 software was used for statistical analysis.

Results: A total of 1131 patients with loop enterostomy in six studies were included in this study; there were 569 cases in the experimental group and 562 cases in the control group. All six studies analyzed the effect of support rods on the incidence of stoma retraction; the meta-analysis showed that in a total of 32 patients, stoma retraction occurred, with a total incidence of about 2.8% in 1131 patients. The incidence of stoma retraction in the rod group was not significantly lower than that in the non-rod group, and the difference was not statistically significant (OR = 0.65, 95% CI 0.32~1.32, I = 0%, P = 0.23), and the studies were homogeneous. The incidences of stoma necrosis (OR = 6.41, 95% CI 2.22~18.55, I = 0%, P = 0.0006), peristomal dermatitis (OR = 2.93, 95% CI 2.01~4.27, I = 0%, P < 0.00001), and mucocutaneous separation (OR = 2.14, 95% CI 1.03~4.47, I = 0%, P = 0.04) were significantly increased in the rod group.

Conclusions: It is not recommended to routinely use stoma support rods in the clinical practice.
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http://dx.doi.org/10.1186/s12957-020-02029-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7584079PMC
October 2020

Global profiling of lysine 2-hydroxyisobutyrylome in Toxoplasma gondii using affinity purification mass spectrometry.

Parasitol Res 2020 Dec 15;119(12):4061-4071. Epub 2020 Oct 15.

State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu Province, People's Republic of China.

Lysine 2-hydroxyisobutyrylation (K) is a recently discovered and evolutionarily conserved form of protein post-translational modification (PTM) found in mammalian and yeast cells. Previous studies have shown that K plays roles in the activity of gene transcription and K-containing proteins are closely related to the cellular metabolism. In this study, a global K-containing analysis using the latest databases (ToxoDB 46, 8322 sequences, downloaded on April 16, 2020) and sensitive immune-affinity enrichment coupled with liquid chromatography-tandem mass spectrometry was performed. A total of 1078 K modification sites across 400 K-containing proteins were identified in tachyzoites of Toxoplasma gondii RH strain. Bioinformatics and functional enrichment analysis showed that K-modified proteins were associated with various biological processes, such as ribosome, glycolysis/gluconeogenesis, and central carbon metabolism. Interestingly, many proteins of the secretory organelles (e.g., microneme, rhoptry, and dense granule) that play roles in the infection cycle of T. gondii were found to be K-modified, suggesting the involvement of K in key biological process during T. gondii infection. We also found that histone proteins, key enzymes related to cellular metabolism, and several glideosome components had K sites. These results expanded our understanding of the roles of K in T. gondii and should promote further investigations of how K regulates gene expression and key biological functions in T. gondii.
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http://dx.doi.org/10.1007/s00436-020-06923-wDOI Listing
December 2020

Corrigendum: gen. nov., sp. nov., a marine bacterium isolated from shrimp gill in a hydrothermal field of Okinawa Trough.

Int J Syst Evol Microbiol 2020 09;70(9):5184

Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, PR China.

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http://dx.doi.org/10.1099/ijsem.0.004400DOI Listing
September 2020

Prevalence of porcine circovirus 2 throughout China in 2015-2019: A systematic review and meta-analysis.

Microb Pathog 2020 Dec 18;149:104490. Epub 2020 Sep 18.

Key Laboratory of Animal Production, Product Quality and Security, Ministry of Education of the People's Republic of China, PR China; Laboratory of Production and Product Application of Sika Deer of Jilin Province, Jilin Agricultural University, Changchun Jilin Province 130118, PR China. Electronic address:

Porcine circovirus type 2 (PCV2) causes infectious diseases in pigs leading to considerable economic losses in the pig industry. To prevent and control PCV2 infections, is important to understand the prevalence and geographical distribution of the virus. We performed the first systematic review and meta-analysis to estimate the prevalence of PCV2 in China. From PubMed, ScienceDirect, Chinese Web of Knowledge, Wanfang, and VIP Chinese Journal, we extracted 53 studies published in China between 2015 and 2019. There were 29,051 samples, 14,230 of which were positive for PCV2. The pooled prevalence of PCV2 was 46.0%, with the highest in Northeastern China (58.1%). The highest prevalence was 86.3% in Xinjiang province. Nursery pigs had the highest prevalence of PCV2 (50.9%), and the serological test detected the highest number of cases (58.5%). PCV2 prevalence was 50.1% in intensive farms and 37.5% in extensive farms. Our findings showed that PCV2 is common throughout China. Effective control measures are necessary to reduce PCV2 infections.
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http://dx.doi.org/10.1016/j.micpath.2020.104490DOI Listing
December 2020

Simultaneous carbon reutilization for primary sludge and stable nitrite production in a hydrolytic acidification coupled with partial denitrification system to treat nitrate contaminant.

Bioresour Technol 2020 Dec 2;318:124062. Epub 2020 Sep 2.

National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.

Partial denitrification (PD, nitrate → nitrite) is a promising process for the hazardous nitrate removal by producing nitrite for Anammox. In this study, the startup and performance of PD using slowly biodegradable organic matter in primary sludge was explored by combining with in-situ hydrolytic acidification (HA). Results showed that efficient PD was established with 61.3% nitrite production at an influent nitrate level of 50 mg/L, with a simultaneous 23.1% reduction in volatile sludge mass. Efficient electron donors including acetate (13.2%), dissolved saccharide (11.9%), and intracellular poly-hydroxyalkanoates (22.5%) were generated from sludge HA, jointly promoting desirable nitrite production. Microbial analysis revealed that adding primary sludge significantly increased community diversity; however, the specific genera Dechloromonas (11.9%) and Thauera (10.5%) remained stably enriched to facilitate the efficient sludge reduction and nitrite production. These findings provide a novel strategy for simultaneously treating primary sludge, nitrate contaminant, and domestic wastewater using a HAPD and Anammox process.
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http://dx.doi.org/10.1016/j.biortech.2020.124062DOI Listing
December 2020

Postoperative morbidity and mortality after mesorectal excision with laparoscopic versus conventional open lateral lymph node dissection for advanced rectal cancer: A meta-analysis.

Asian J Surg 2021 Jan 11;44(1):26-35. Epub 2020 Aug 11.

Department of General Surgery, Northern Jiangsu People's Hospital, Nantong Road No.98, Yangzhou 225001, China. Electronic address:

Lateral lymph node dissection (LLND) is a challenging procedure due to its technical difficulty and higher incidence of surgical morbidity, thus the purpose of this study is to compare the postoperative morbidity and mortality between laparoscopic and conventional open LLND after mesorectal excision (ME) for advanced rectal cancer. A comprehensive search was conducted in August 2019 based on data from PubMed, Embase, Cochrane Library, CNKI and Wanfang Data to identify relevant studies, and studies comparing laparoscopic with conventional open LLND were included. Seven studies with 335 cases in the laparoscopic LLND group and 841 cases in the conventional open LLND group were finally included. Compared with the conventional open LLND group, the laparoscopic LLND group enjoys a lower postoperative morbidity (OR = 0.47,95% CI [0.23, 0.97], P = 0.04), same postoperative mortality (Postoperative mortality in both groups was zero), shorter length of postoperative hospitalization (WMD = -5.30, 95% CI [-8.42, -2.18], P = 0.0009), less wound infection (OR = 0.40,95% CI [0.21, 0.77], P = 0.006), less intestinal obstruction (OR = 0.50,95% CI [0.29, 0.84], P = 0.009), and less urinary retention (OR = 0.61,95% CI [0.38, 0.97], P = 0.04). There were no significant differences in the incidence of anastomotic leakage, abdominal pelvic abscess and urinary tract infection between the two groups (P > 0.05). But in the sensitivity analysis, there was a significant change of urinary tract infection (OR = 0.22, 95%CI [0.06, 0.89], P = 0.03), and the degree of between-study heterogeneity decreased greatly. In conclusion, laparoscopic LLND may be a better alternative to conventional open LLND for advanced rectal cancer with lower postoperative morbidity and shorter postoperative hospital stay.
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http://dx.doi.org/10.1016/j.asjsur.2020.06.010DOI Listing
January 2021

Exploration of DNA Methylation-Driven Genes in Papillary Thyroid Carcinoma Based on the Cancer Genome Atlas.

J Comput Biol 2021 Jan 13;28(1):99-114. Epub 2020 Aug 13.

Department of Medical Ultrasound, Affiliated Hospital of Jiangsu University, Zhenjiang, People's Republic of China.

Although the incidence of thyroid carcinoma is reported to be the highest among malignancies of endocrine system, its diagnosis is still unsatisfactory. This study sought to explore the key DNA methylation-driven genes in the development of papillary thyroid carcinoma (PTC) via a bioinformatic analysis based on the Cancer Genome Atlas (TCGA) database and was validated using the Gene Expression Omnibus (GEO) database. The level 3 DNA methylation, mRNA expression, and clinical data of 499 patients with PTC were obtained from the TCGA database. The R package LIMMA, edgeR, and MethylMix were applied to explore the DNA methylation-driven genes in PTC. The ConsensusPathDB software, DAVID, and STRING databases were used for Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analyses, as well as protein/protein interaction network construction individually. To verify the result, the explored genes were validated using GSE97466 data set retrieved from the GEO database. Fifty-seven (57) methylation-driven genes were detected via MethylMix based on a beta mixture model that compared the DNA methylation state of tumor tissues with that of the normal tissues. Eventually, three genes (TNFRSF1A, CLDN1, and CASP1) were identified to be the most potential biomarkers for the diagnosis or treatment of PTC. These results suggest the crucial roles of TNFRSF1A, CLDN1, and CASP1 in the tumorigenesis of PTC and provide a vital bioinformatic basis for further experimental validations and clinical applications.
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http://dx.doi.org/10.1089/cmb.2019.0471DOI Listing
January 2021

Development of a denitrification system using primary sludge as solid carbon source - Potential to couple with anammox process.

Sci Total Environ 2020 Oct 17;737:140315. Epub 2020 Jun 17.

Advanced Environmental Biotechnology Centre, Nanyang Environment and Water Research Institute, Nanyang Technological University, 1 Cleantech Loop, Singapore 637141, Singapore; School of Civil and Environmental Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore. Electronic address:

Heterotrophic denitrification is a robust and reliable process for nitrogen removal from wastewater. However, wastewater often faces the issue of lacking carbon source. In this study, the feasibility of using primary sludge, a by-product of wastewater treatment plants, to support denitrification of high-strength nitrite wastewater was investigated. Results suggest the desired performance can be achieved with the influent nitrite concentration of 400 to 1200 mg N/L, and the optimal primary sludge dosage for the complete nitrite removal was 3.6 g VSS/g N. Ammonium removal was also detected along with nitrite removal. Microbial analysis reveals various types of denitrifying bacteria and a large number of macromolecular organics degrading bacteria existed in the microbial community. Notably, anammox bacteria, Candidatus Brocadia, was also identified with an abundance of 0.1%. The slow kinetics of carbon source release from primary sludge was likely the reason for the existence of anammox process. This study developed a promising nitrogen removal process using an alternative carbon source for denitrification, and it shows great potential to couple denitrification with anammox to reduce ammonium residue.
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http://dx.doi.org/10.1016/j.scitotenv.2020.140315DOI Listing
October 2020

Successful establishment of partial denitrification by introducing hydrolytic acidification of slowly biodegradable organic matter.

Bioresour Technol 2020 Nov 22;315:123887. Epub 2020 Jul 22.

National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.

Partial denitrification (PD, nitrate → nitrite) was successfully established in this study by introducing hydrolytic acidification (HA) of slowly biodegradable organic matter (SBOM). A high selectivity for the nitrate over nitrite as electron acceptors was obtained during a 178-day long-term operation, with the nitrate to nitrite transformation ratio climbing to 81.3% at an influent SBOM of 286 mg/L and low-strength nitrate of 40 mg/L. Acetate (33.9%) and dissolved saccharide (19.3%), as the major SBOM HA products, indeed facilitated high-efficiency nitrite production by serving as favorable electron donors. This was well explained by the metagenomic analysis that the dominant Dechloromonas and Thauera denitrifying genera, which hold 3.9 times higher abundance of nitrate reductase than nitrite reductase, also played a key role in carbon glycolysis and acidification. This study provides new insight into PD development in multiple types of wastewater for the versatile carbon/nitrogen metabolism of functional bacteria.
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http://dx.doi.org/10.1016/j.biortech.2020.123887DOI Listing
November 2020

Taxonomy and phylogeny of (Hymenochaetales, Basidiomycota): four new species and keys to species of the genus.

MycoKeys 2020 7;68:115-135. Epub 2020 Jul 7.

Shandong Provincial Key Laboratory of Applied Mycology, Qingdao Agricultural University, Qingdao 266109, China Qingdao Agricultural University Qingdao China.

is a polypore genus with white to cream or buff basidiomata, whose species in Hymenochaetales are poorly known. We study the phylogeny and diversity of based on our recent collections from tropic and subtropic Asian-Pacific regions. Phylogenetic analyses based on the internal transcribed spacer (ITS) and nuclear large subunit (nLSU) ribosomal RNA gene regions indicate that ten terminal lineages are well supported within . Based on morphological examination and phylogeny, four new species, viz. , , and are described, and a new combination, , is proposed. All these species are illustrated. is characterized by tiny basidiomata with bluish pores when fresh, generative hyphae dominating at the dissepiment edges, the presence of cystidioles, and allantoid basidiospores measuring 3.8-4.4 × 0.9-1.3 μm. differs from other poroid species in the genus by having parallel tramal hyphae in combination with lunate basidiospores measuring 2.8-3.3 × 0.9-1.2 μm. have generative and skeletal hyphae co-dominating at the dissepiment edges, and lunate basidiospores measuring 3.5-4 × 1-1.3 μm. is distinguished by small pores (8-10 per mm) and relatively long allantoid basidiospores measuring 4.2-5 × 0.8-1 μm. is characterized by buff to olivaceous buff basidiomata when dry, 5-7 pores per mm, rosette-like crystals rare, and allantoid basidiospores measuring 3.7-4.3 × 1-1.3 μm. An identification key to all accepted species is provided.
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http://dx.doi.org/10.3897/mycokeys.68.53561DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7360635PMC
July 2020

Ceftriaxone Relieves Trigeminal Neuropathic Pain Through Suppression of Spatiotemporal Synaptic Plasticity Restoration of Glutamate Transporter 1 in the Medullary Dorsal Horn.

Front Cell Neurosci 2020 30;14:199. Epub 2020 Jun 30.

Department of Pain Management, The Third Xiangya Hospital, Institute of Pain Medicine, Central South University, Changsha, China.

Using a rat model of trigeminal neuropathic pain (TNP) produced by chronic compression of the infraorbital nerve (CCI-ION), we investigated the analgesic effect and the underlying mechanisms of ceftriaxone (Cef), a β-lactam antibiotic, that is thought to be a potent stimulator of glutamate transporter 1 (GLT-1). First, repeated intraperitoneal (i.p.) injections of Cef (200 mg/kg) for 5-days since Day 1 of CCI-ION could significantly relieve both mechanical and thermal pain hypersensitivity from day 10 after drug administration. Western blot and immunofluorescent results demonstrated that 5-days administration of Cef resulted in the restoration of GLT-1 expression to a level equivalent to the sham control which was dramatically lost under the TNP condition. Moreover, multi-electrode (8 × 8) array recordings of network field excitatory postsynaptic potentials (fEPSPs) were performed on the acutely dissociated medullary dorsal horn slice evoked by electrical stimulation of the trigeminal spinal tract. The results showed that the increased number of fEPSPs, induction rate, and maintenance of long-term potentiation caused by CCI-ION were significantly suppressed by 5-days administration of Cef. Taken together, the results indicate that Cef can relieve TNP through suppression of spatiotemporal synaptic plasticity GLT-1 restoration in the medullary dorsal horn of the trigeminal nerve.
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http://dx.doi.org/10.3389/fncel.2020.00199DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7340123PMC
June 2020

Image-Forming Visual Basis of Empathy for Pain in Mice.

Neurosci Bull 2020 Dec 20;36(12):1563-1569. Epub 2020 Jun 20.

Institute for Biomedical Sciences of Pain, Tangdu Hospital, The Fourth Military Medical University, Xi'an, 710038, China.

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http://dx.doi.org/10.1007/s12264-020-00528-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7719150PMC
December 2020

Early life famine exposure, adulthood obesity patterns and the risk of nonalcoholic fatty liver disease.

Liver Int 2020 11 19;40(11):2694-2705. Epub 2020 Jul 19.

Shanghai National Clinical Research Center for Metabolic Diseases, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Institute of Endocrine and Metabolic Diseases, Shanghai, China.

Background: Early life exposure to famine and adulthood obesity increased the risk of nonalcoholic fatty liver disease (NAFLD) in adulthood. However, the joint effects on adulthood NAFLD risk are not clear.

Aim: This study aimed to explore the joint effects of famine exposure and adulthood obesity on NAFLD risk in later life.

Methods: We included 7632 subjects aged ≥40 years from a community-dwelling population. Participants were divided into 4 famine exposure groups according to the birth year, including nonexposed (1963-1974), fetal-exposed (1959-1962), childhood-exposed (1949-1958) and adolescent-exposed (1941-1948). General obesity was assessed by body mass index (BMI: overweight ≥24.0 kg/m , obesity ≥28.0 kg/m ) and abdominal obesity assessed by waist-to-hip ratio (WHR, men/women: moderate ≥0.90/0.85, high ≥0.95/0.90).

Results: Compared with nonexposed, fetal- and childhood-exposed participants show an increased risk of NAFLD with multivariable-adjusted odds ratio (OR) and 95% confidence interval (CI) of 1.28 (1.02-1.61) and 1.40 (1.04-1.88) respectively. After further adjusting BMI and WHR, the increased risk was observed only in childhood-exposed participants (OR = 1.46, 95% CI = 1.04-2.05). Significant interaction between famine exposure and general obesity on the risk of NAFLD was observed in women (P for interaction = .02). No significant interactions were detected between famine exposure and abdominal obesity (all P for interaction >.05). Compared with normal-BMI and -WHR participants, those with both general and abdominal obesity in adulthood had 20.74 (95% CI: 12.00-35.96), 14.45 (8.76-23.86), 23.02 (16.28-32.57) and 13.04 (8.30-20.48)-fold higher risk in nonexposed, fetal-, childhood- and adolescent-exposed groups respectively.

Conclusion: Coexistence of early life famine exposure and adulthood obesity was associated with a higher risk of NAFLD.
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http://dx.doi.org/10.1111/liv.14572DOI Listing
November 2020

Laparoscopy-assisted pylorus-preserving gastrectomy versus laparoscopy-assisted distal gastrectomy for early gastric cancer in perioperative outcomes: A meta-analysis.

Asian J Surg 2020 Aug 8;43(8):862-863. Epub 2020 Jun 8.

Department of General Surgery, Northern Jiangsu Province Hospital, Clinical Medical College, Yangzhou University, Institute of General Surgery - Yangzhou University, Yangzhou, China. Electronic address:

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http://dx.doi.org/10.1016/j.asjsur.2020.05.009DOI Listing
August 2020

[Expression of SFRP1 and MIF in elderly patients with severe periodontitis and its correlation with cognitive function].

Shanghai Kou Qiang Yi Xue 2020 Feb;29(1):97-101

Department of Periodontics, Qingdao Stomatological Hospital. Qingdao 266000, Shangdong Province, China.

Purpose: To investigate the expression of secreted frizzle-related protein 1 (SFRP1) and macrophage migration inhibitory factor (MIF) in elderly patients with severe periodontitis and its correlation with cognitive function.

Methods: Thirty-two elderly patients with periodontitis in Qingdao Stomatological Hospital from February 2018 to February 2019 were enrolled, and divided into two groups according to the severity: mild group and severe group. All selected subjects received periodontal examination and Montreal cognitive assessment (MoCA).The expression of SFRP1 and MIF in serum was also determined. Then the correlations among SFRP1 and MIF periodontal index and cognitive function was analyzed. The data were processed by SPSS 20.0 software package.

Results: The probing depth (PD), clinical attachment level (CAL), sulcus bleeding index (SBI) and gingival crevicular fluid (GCF) showed significant difference between the two groups (P<0.05). The serum levels of SFRP1 and MIF in the severe group were significantly higher than those in the mild group (P<0.05). Serum SFRP1 level was positively correlated with MIF (P<0.05). Serum SFRP1 and MIF levels were positively correlated with periodontal index (P<0.05). The MoCA score of the severe group was significantly lower than that of the mild group (P<0.05). Serum SFRP1 and MIF levels were negatively correlated with MoCA score (P<0.05).

Conclusions: SFRP1 and MIF are highly expressed in serum and gingival tissues of elderly patients with severe periodontitis, and are closely related to the degree of periodontal damage. Meanwhile, patients with periodontitis may have some degree of cognitive dysfunction, and SFRP1 and MIF may affect the periodontal tissue structure through Wnt/β-catenin signal pathway and participate in the occurrence and development of cognitive dysfunction.
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February 2020

Early Life Famine Exposure, Ideal Cardiovascular Health Metrics, and Risk of Incident Diabetes: Findings From the 4C Study.

Diabetes Care 2020 08 4;43(8):1902-1909. Epub 2020 Jun 4.

Shanghai National Clinical Research Center for Metabolic Diseases, Key Laboratory for Endocrine and Metabolic Diseases of the National Health Commission of the PR China, Shanghai National Center for Translational Medicine, Shanghai Institute of Endocrine and Metabolic Diseases, Department of Endocrine and Metabolic Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Objective: We aim to investigate the impact of ideal cardiovascular health metrics (ICVHMs) on the association between famine exposure and adulthood diabetes risk.

Research Design And Methods: This study included 77,925 participants from the China Cardiometabolic Disease and Cancer Cohort (4C) Study who were born around the time of the Chinese Great Famine and free of diabetes at baseline. They were divided into three famine exposure groups according to the birth year, including nonexposed (1963-1974), fetal exposed (1959-1962), and childhood exposed (1949-1958). Relative risk regression was used to examine the associations between famine exposure and ICVHMs on diabetes.

Results: During a mean follow-up of 3.6 years, the cumulative incidence of diabetes was 4.2%, 6.0%, and 7.5% in nonexposed, fetal-exposed, and childhood-exposed participants, respectively. Compared with nonexposed participants, fetal-exposed but not childhood-exposed participants had increased risks of diabetes, with multivariable-adjusted risk ratios (RRs) (95% CIs) of 1.17 (1.05-1.31) and 1.12 (0.96-1.30), respectively. Increased diabetes risks were observed in fetal-exposed individuals with nonideal dietary habits, nonideal physical activity, BMI ≥24.0 kg/m, or blood pressure ≥120/80 mmHg, whereas significant interaction was detected only in BMI strata ( for interaction = 0.0018). Significant interactions have been detected between number of ICVHMs and famine exposure on the risk of diabetes ( for interaction = 0.0005). The increased risk was observed in fetal-exposed participants with one or fewer ICVHMs (RR 1.59 [95% CI 1.24-2.04]), but not in those with two or more ICVHMs.

Conclusions: The increased risk of diabetes associated with famine exposure appears to be modified by the presence of ICVHMs.
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http://dx.doi.org/10.2337/dc19-2325DOI Listing
August 2020

Global Proteomic Analysis of Lysine Malonylation in .

Front Microbiol 2020 28;11:776. Epub 2020 Apr 28.

State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.

Lysine malonylation (Kmal) is a new post-translational modification (PTM), which has been reported in several prokaryotic and eukaryotic species. Although Kmal can regulate many and diverse biological processes in various organisms, knowledge about this important PTM in the apicomplexan parasite is limited. In this study, we performed the first global profiling of malonylated proteins in tachyzoites using affinity enrichment and Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Three experiments performed in tandem revealed 294, 345, 352 Kmal sites on 203, 236, 230 malonylated proteins, respectively. Computational analysis showed the identified malonylated proteins to be localized in various subcellular compartments and involved in many cellular functions, particularly mitochondrial function. Additionally, one conserved Kmal motif with a strong bias for cysteine was detected. Taken together, these findings provide the first report of Kmal profile in and should be an important resource for studying the physiological roles of Kmal in this parasite.
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http://dx.doi.org/10.3389/fmicb.2020.00776DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7198775PMC
April 2020

Serum uric acid and risk of incident diabetes in middle-aged and elderly Chinese adults: prospective cohort study.

Front Med 2020 Dec 29;14(6):802-810. Epub 2020 Apr 29.

Department of Endocrine and Metabolic Diseases, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.

The association between serum uric acid and the risk of incident diabetes in Chinese adults remains unknown. This study aimed to investigate this association in a community-dwelling population aged ≥ 40 years in Shanghai, China. Oral glucose tole3rance test was conducted during baseline and follow-up visits. Relative risk regression was utilized to examine the associations between baseline gender-specific serum uric acid levels and incident diabetes risk. A total of 613 (10.3%) incident diabetes cases were identified during the follow-up visit after 4.5 years. Fasting plasma glucose, postload glucose, and glycated hemoglobin A1c during the follow-up visit progressively increased across the sex-specific quartiles of serum uric acid (all Ps < 0.05). The incidence rate of diabetes increased across the quartiles of serum uric acid (7.43%, 8.77%, 11.47%, and 13.43%). Multivariate adjusted regression analysis revealed that individuals in the highest quartile had 1.36-fold increased risk of diabetes compared with those in the lowest quartile of serum uric acid (odds ratio (95% confidence interval) = 1.36 (1.06-1.73)). Stratified analysis indicated that the association was only observed in women. Accordingly, serum uric acid was associated with the increased risk of incident diabetes among middle-aged and elderly Chinese women.
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http://dx.doi.org/10.1007/s11684-019-0723-7DOI Listing
December 2020