Publications by authors named "Rui Ding"

253 Publications

Convenient Preparation of -Acylbenzoxazines from Phenols, Nitriles, and DMSO Initiated by a Catalytic Amount of (COCl).

J Org Chem 2021 Apr 17;86(7):4932-4943. Epub 2021 Mar 17.

Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Key Laboratory of Flavor Chemistry, Beijing Technology and Business University, Beijing 100048, China.

A convenient preparation method of -acylbenzoxazines has been developed, in which phenols react with nitriles and dimethyl sulfoxide (DMSO) in the presence of a catalytic amount of (COCl) in CHCN or chloroform to afford the corresponding -acylbenzoxazines in moderate-to-good yields. DMSO acts as a source of HCHO, which is generated in situ from the decomposition of a methoxydimethylsulfonium salt. A regeneration cycle of the methoxydimethylsulfonium salt is proposed, which is initiated by a catalytic amount of (COCl).
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http://dx.doi.org/10.1021/acs.joc.0c02768DOI Listing
April 2021

Correlation between in vitro fertilization and artificial insemination in Holstein bulls.

Anim Biosci 2021 Feb 16. Epub 2021 Feb 16.

College of Life Science, Inner Mongolia University, Hohhot 010070, P.R. China.

Objective: Owing to the lack of breeding index for efficient and quick fertility evaluations of Holstein bulls when using traditional or genome-wide detection methods, this study aimed to determine whether in vitro fertilization (IVF) could be used as an indicator of conception rate of artificial insemination (AI).

Methods: Conventional and sexed frozen semen from nine bulls were used for IVF and AI.

Results: The IVF and AI conception rates of each bull were confirmed to be positively correlated between the conventional frozen and sexed frozen semen. The correlation coefficient R values of nine bulls between IVF and AI methods were 0.73 and 0.97 for the conventional frozen and sexed frozen semen, respectively. The average conception rate of three bulls undergoing AI was 69.5% and 64.2%, 61.8% and 58.8%, and 48.2% and 46.2% in first-, second-, and third-born cows when conventional frozen and sexed frozen semen were used, respectively, which showed a positive correlation with the fertilization rate in the same parity. We propose an evaluation standard to assess the fertilization ability of bulls based on their IVF test results, which is categorized into three grades: grade one, normal fertility bull with an AI conception rate of 40% ± 5% and IVF rate of 45%-60%; grade two, higher fertility bull with an AI conception rate of 50% ± 5% and IVF rate of 61%-80%; and grade three, highest fertility bull with an AI conception rate of 60% ± 5% and IVF rate of >80%.

Conclusion: These findings reveal that IVF results can be used as a breeding index for bulls to evaluate their AI conception ability, which may shorten the time required to select bulls for breeding.
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http://dx.doi.org/10.5713/ab.20.0665DOI Listing
February 2021

Ginkgolide A attenuates sepsis-associated kidney damage via upregulating microRNA-25 with NADPH oxidase 4 as the target.

Int Immunopharmacol 2021 Mar 4;95:107514. Epub 2021 Mar 4.

Department of Urology, Wuxi Traditional Chinese Medicine Hospital, Wuxi, China. Electronic address:

The aim of the present study was to explore the effects of Ginkgolide A (GA) on renal function of mice with sepsis and whether GA could attenuate sepsis-associated inflammation and apoptosis in kidney via upregulating microRNA (miR)-25 with NADPH oxidase 4 (Nox4) as the target. Experiments were carried out on lipopolysaccharide (LPS)-treated mice and kidney tubular (NRK-52E) cells. GA significantly inhibited the increases of creatinine (Cr), blood urea nitrogen (BUN) and cystatin C (CysC) in the serum of LPS-treated mice. The increases of inflammatory factors including tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6 in the kidneys of LPS-treated mice or NRK-52E cells were inhibited by GA administration. The changes of cleaved-caspase 3, cleaved-caspase 8, Bax, Bcl2 in mouse kidney and NRK-52E cells treated by LPS were reversed by GA administration. The sepsis-induced decrease of miR-25 was enhanced by GA treatment. The LPS-induced increases of inflammatory factors and apoptosis in mouse kidney or NRK-52E cells were attenuated after miR-25 agomiR administration. The bioinformatics analysis and luciferase reporter assays showed that Nox4 was a direct target gene of miR-25. Treatment with miR-25 inhibited Nox4 expression, while Nox4 over-expression reversed the inhibiting effects of miR-25 agomiR on LPS-induced increases of inflammatory factors and apoptosis in NRK-52E cells. These results indicated that GA could improve sepsis-induced renal damage by attenuating renal inflammation and apoptosis via upregulating miR-25 with Nox4 as the target.
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http://dx.doi.org/10.1016/j.intimp.2021.107514DOI Listing
March 2021

Complete mitochondrial genome of the edible Basidiomycete mushroom (Aphyllophorales: Thelephoraceae) from China.

Mitochondrial DNA B Resour 2021 Feb 20;6(2):606-607. Epub 2021 Feb 20.

Gardening and Horticulture Department, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla, China.

The complete mitochondrial genome of , an edible Basidiomycete mushroom species with ecological and economic value is reported in this study. The whole genome is a circular molecule 50,672 bp in length and encodes 42 genes as follows: 15 protein-coding genes, two rRNA genes and 25 tRNA genes. The A, T, C, G contents in the genome are 35.60%, 35.31%, 13.89%, and 15.20%, respectively. Phylogenetic analysis revealed a close relationship between and . This is the first complete mitochondrial genome for that will be useful for providing basic genetic information for this important species.
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http://dx.doi.org/10.1080/23802359.2020.1869620DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7899663PMC
February 2021

The investigation of surface composition of nitrogen-doped niobium for superconducting RF cavities.

Nanotechnology 2021 Mar 3. Epub 2021 Mar 3.

Sichuan University, Chengdu, Sichuan, CHINA.

Systematic analysis of the surface morphology, crystalline phase, chemical composition and elemental distribution along depth for nitrogen-doped niobium was carried out through different ways of characterization, including SEM, AFM, GIXRD, RBS and layer-by-layer XPS analysis. The results showed that, after nitrogen doping, the surface was covered by densely distributed trigonal precipitates with average crystallite size of 32 ± 8 nm, in line with the calculation result (29.9 nm) of nitrogen-enriched β-Nb2N from GIXRD, demonstrating the phase composion of trigonal precipitates. The depth analysis through RBS and XPS indicated that β-Nb2N was dominant in the topmost 9.7 nm and extended to depth of 575 nm with gradually decreased content. In addition, the successive change of naturally oxidized states of niobium after nitrogen doping along depth was revealed along depth. It was interesting to find that oxygen diffusion depth could be moderately enhanced by nitridation process. These results established the near-surface phase composition of nitrided niobium, which was of great significance for evaluating the effect of nitrogen doping and further understanding the Q improvement of the SRF cavities.
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http://dx.doi.org/10.1088/1361-6528/abeb99DOI Listing
March 2021

Combination of Polydopamine Coating and Plasma Pretreatment to Improve Bond Ability Between PEEK and Primary Teeth.

Front Bioeng Biotechnol 2020 29;8:630094. Epub 2021 Jan 29.

Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an, China.

Preformed crowns are preferred to reduce the failure risk of restoration of primary teeth, but some drawback of conventional material is still a main barrier for their clinical use. Polyether etherketone (PEEK), a tooth colored, high-performance thermoplastic polymer, has been recognized as a promising alternative to manufacture the restoration of primary teeth. However, the hydrophobic surface and low surface energy of PEEK make it hard to establish a strong and durable adhesion. In this study, we have evaluated a modification method of polydopamine (PDA) coating with plasma pretreatment for the PEEK films by physical and chemical characterization, bonding properties, and biocompatibility. The surface properties of PEEK were well-characterized by scanning electron microscope (SEM) and X-ray photoelectron spectroscopy (XPS). The adhesive strength of the PEEK films was greatly improved without significant reduction of the proliferation rate of human gingival fibroblast cells in MTT and Live/Dead assays. Therefore, PDA coating with plasma pretreatment may give a new solution for effective clinical application of PEEK in primary performed crowns.
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http://dx.doi.org/10.3389/fbioe.2020.630094DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7880054PMC
January 2021

DNA-templated silver nanoclusters as an efficient catalyst for reduction of nitrobenzene derivatives: a systematic study.

Nanotechnology 2021 May;32(19):195705

School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Wenyuan Road 1, Nanjing 210023, People's Republic of China.

Nitrobenzene compounds are highly toxic pollutants with good stability, and they have a major negative impact on both human health and the ecological environment. Herein, it was found for the first time that fluorescent DNA-silver nanoclusters (DNA-AgNCs) can catalyze the reduction of toxic and harmful nitro compounds into less toxic amino compounds with excellent tolerance to high temperature and organic solvents. In this study, the reduction of p-nitrophenol (4-NP) as a model was systematically investigated, followed by expending the substrate to disclose the versatility of this reaction. This report not only expanded the conditions for utilizing catalytic reduction conditions of DNA-AgNCs as an efficient catalyst in the control of hazardous chemicals but also widened the substrate range of DNA-AgNCs reduction, providing a new angle for the application of noble metal nanoclusters.
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http://dx.doi.org/10.1088/1361-6528/abe3b4DOI Listing
May 2021

Stabilization of Organic Cathodes by a Temperature-Induced Effect Enabling Higher Energy and Excellent Cyclability.

ACS Appl Mater Interfaces 2021 Feb 4;13(6):7178-7187. Epub 2021 Feb 4.

Key Laboratory of Environmentally Friendly Chemistry and Application of Ministry of Education, Key Laboratory for Green Organic Synthesis and Application of Hunan Province, College of Chemistry, Xiangtan University, 411105 Xiangtan, China.

To face the challenge of all-climate application, organic rechargeable batteries must hold the capability of efficiently operating both at high temperatures (>50 °C) and low temperatures (-20 °C). However, the low electronic conductivity and high solubility of organic molecules significantly impede the development in electrochemical energy storage. This issue can be effectively diminished using functionalized porphyrin complex-based organic cathodes by the in-situ electropolymerization of electrodes at elevating temperatures during electrochemical cycling. [5,15-(ethynyl)-10,20-diphenylporphinato]copper(II) (CuDEPP)- and 5,15-(ethynyl)-10,20-diphenylporphinato (DEPP)-based cathodes are proposed as models, and it is proved that a largely improved electrochemical performance is observed in both cathodes at a high operating temperature. Reversible capacities of 249 and 105 mA h g are obtained for the CuDEPP and DEPP cathodes after 1000 cycles at 50 °C, respectively. The result indicates that the temperature-induced in situ electropolymerization strategy responds to the enhanced electrochemical performance. This study would open new opportunities for developing highly stable organic cathodes for electrochemical energy storage even at high temperatures.
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http://dx.doi.org/10.1021/acsami.0c20525DOI Listing
February 2021

Rapid Production of a Novel Al(III) Dependent Bioflocculant Isolated From 160-1 and Its Application Combined With Inorganic Salts.

Front Microbiol 2020 12;11:622365. Epub 2021 Jan 12.

School of Life Sciences, University of Science and Technology of China, Hefei, China.

An efficient bioflocculant-producing strain, 160-1, was identified by 16S rRNA and mass spectrometry analyses. Rapid production of bioflocculant EPS-160 was obtained with 10.01 g/(L⋅d) after optimized by response surface methodology. With the aid of Al(III), more than 90% flocculation activity of EPS-160 at 8 mg/L dosage was achieved in 5 min. Thus, this novel Al(III) dependent bioflocculant was used in combined with chemical coagulants AlCl to remove kaolin suspensions and wastewater treatment. The results indicated that the addition of EPS-160 in aggregation system not only largely improved the flocculation ability than the individual use of chemical flocculant (over 30 percent), but also overcome the decrease of flocculation activity due to the overdose of AlCl and maintained the optimum dosage of AlCl in a wide range (11-23 mg/L). The zeta potentials and EPS-160 structure indicated that both charge neutralization and bridging were the flocculation mechanism with kaolin. During the wastewater treatment, this composite flocculants consisted of EPS-160 and AlCl also had great performance for turbidity elimination. Moreover, with the properties of high flocculation activity, hyperthermal stability, pH tolerance and non-toxicity, EPS-160 shows great potential applications.
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http://dx.doi.org/10.3389/fmicb.2020.622365DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7835285PMC
January 2021

Heat shock protein 22 modulates NRF1/TFAM-dependent mitochondrial biogenesis and DRP1-sparked mitochondrial apoptosis through AMPK-PGC1α signaling pathway to alleviate the early brain injury of subarachnoid hemorrhage in rats.

Redox Biol 2021 Apr 6;40:101856. Epub 2021 Jan 6.

Neurosurgery Center, Department of Cerebrovascular Surgery, Engineering Technology Research Center of Education Ministry of China on Diagnosis and Treatment of Cerebrovascular Disease, Zhujiang Hospital, Southern Medical University, Guangzhou, 510280, Guangdong, China; Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, 510280, Guangdong, China. Electronic address:

Mitochondrial dysfunction has been widely accepted as a detrimental factor in subarachnoid hemorrhage (SAH)-induced early brain injury (EBI), which is eminently related to poor neurologic function outcome. Previous studies have revealed that enhancement of heat shock protein 22 (hsp22) under conditions of stress is a friendly mediator of mitochondrial homeostasis, oxidative stress and apoptosis, thus accelerating neurological recovery. However, no study has confirmed whether hsp22 attenuates mitochondrial stress and apoptosis in the setting of SAH-induced EBI. Our results indicated that endogenous hsp22, p-AMPK/AMPK, PGC1α, TFAM, Nrf1 and Drp1 were significantly upregulated in cortical neurons in response to SAH, accompanied by neurologic impairment, brain edema, neuronal degeneration, lower level of mtDNA and ATP, mitochondria-cytosol translocation of cytochrome c, oxidative injury and caspase 3-involved mitochondrial apoptosis. However, exogenous hsp22 maintained neurological function, reduced brain edema, improved oxidative stress and mitochondrial apoptosis, these effects were highly dependent on PGC1α-related mitochondrial biogenesis/fission, as evidenced by co-application of PGC1α siRNA. Furthermore, we demonstrated that blockade of AMPK with dorsomorphin also compromised the neuroprotective actions of hsp22, along with the alterations of PGC1α and its associated pathway molecules. These data revealed that hsp22 exerted neuroprotective effects by salvaging mitochondrial function in an AMPK-PGC1α dependent manner, which modulates TFAM/Nrf1-induced mitochondrial biogenesis with positive feedback and DRP1-triggered mitochondrial apoptosis with negative feedback, further reducing oxidative stress and brain injury. Boosting the biogenesis and repressing excessive fission of mitochondria by hsp22 may be an efficient treatment to relieve SAH-elicited EBI.
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http://dx.doi.org/10.1016/j.redox.2021.101856DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816003PMC
April 2021

Mixed Modification of the Surface Microstructure and Chemical State of Polyetheretherketone to Improve Its Antimicrobial Activity, Hydrophilicity, Cell Adhesion, and Bone Integration.

ACS Biomater Sci Eng 2020 02 7;6(2):842-851. Epub 2020 Jan 7.

Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, P. R. China.

Polyetheretherketone (PEEK) is becoming an attractive surgical implant material in the biomedical field. However, its hydrophobicity and biological inertia have seriously hindered its development in the field of biomaterials and application in clinic. In this work, a mixed modification approach of the surface structure and chemical state was proposed to improve hydrophilicity and bioactivity of PEEK and meanwhile endowed it with antimicrobial properties. First, the effect of mixed acids of nitric acid and concentrated sulfuric acid with different volume ratios on the surface morphology of PEEK was quantitatively investigated, so as to obtain an optimal mixed acid of nitric acid and concentrated sulfuric acid with a volume ratio that could create a multilevel porous structure. Based on this, chemical grafting of ethylenediamine was conducted to achieve amination on the surface of the PEEK. The results showed that such double modification of the surface structure and chemical state could endow PEEK not only with antimicrobial activity but also with good hydrophilicity, cell compatibility, and bioactivity, which were beneficial to improve the bone integration ability of PEEK greatly.
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http://dx.doi.org/10.1021/acsbiomaterials.9b01148DOI Listing
February 2020

Effect of Electrospun Silk Fibroin-Silk Sericin Films on Macrophage Polarization and Vascularization.

ACS Biomater Sci Eng 2020 06 20;6(6):3502-3512. Epub 2020 May 20.

Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, People's Republic of China.

Biomaterial implantation is followed by an inflammatory cascade dominated by the macrophages, which polarized to the proinflammation M1 phenotype or prohealing M2 phenotype. Generally, silk sericin (SS) is considered to be of high immunogenicity associated with native silk fibers. The blends of silk fibroin (SF) and SS in different mass ratios might elicit different host immune responses and induce macrophage phenotype switch. The objective of this study was to assess the effects of electrospun SF-SS fibrous films with different mass ratios (10:0, 9:1, 8:2, and 7:3) on the macrophage phenotypes and explore the optimal ratio of SF and SS for angiogenesis. Our results indicated that the macrophages were activated by the addition of SS. When the mass ratio of SF and SS reached 7:3, the film displayed the highest degree of vascularization. The macrophages were induced to secrete more M1 and M2 cytokines accompanying with high M2/M1 ratio. Taken together, this study provided a perspective to promote neovascularization by modulating appropriate host response and macrophage phenotypes in tissue engineering field.
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http://dx.doi.org/10.1021/acsbiomaterials.0c00175DOI Listing
June 2020

Transcutaneous Electrical Acupoint Stimulation in Early Life Changes Synaptic Plasticity and Improves Symptoms in a Valproic Acid-Induced Rat Model of Autism.

Neural Plast 2020 29;2020:8832694. Epub 2020 Dec 29.

Department of Neurobiology, School of Basic Medical Sciences, Peking University, Beijing, China.

Autism spectrum disorder (ASD) is a developmental disorder characterized by social behavior deficit in childhood without satisfactory medical intervention. Transcutaneous electrical acupoint stimulation (TEAS) is a noninvasive technique derived from acupuncture and has been shown to have similar therapeutic effects in many diseases. Valproic acid- (VPA-) induced ASD is a known model of ASD in rats. The therapeutic efficacy of TEAS was evaluated in the VPA model of ASD in the present study. The offspring of a VPA-treated rat received TEAS in the early life stage followed by a series of examinations conducted in their adolescence. The results show that following TEAS treatment in early life, the social and cognitive ability in adolescence of the offspring of a VPA rat were significantly improved. In addition, the abnormal pain threshold was significantly corrected. Additional studies demonstrated that the dendritic spine density of the primary sensory cortex was decreased with Golgi staining. Results of the transcriptomic study showed that expression of some transcription factors such as the neurotrophic factor were downregulated in the hypothalamus of the VPA model of ASD. The reduced gene expression was reversed following TEAS. These results suggest that TEAS in the early life stage may mitigate disorders of social and recognition ability and normalize the pain threshold of the ASD rat model. The mechanism involved may be related to improvement of synaptic plasticity.
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http://dx.doi.org/10.1155/2020/8832694DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7787794PMC
December 2020

Anti-HCMV activity by an irreversible p97 inhibitor LC-1310.

Med Chem Res 2021 Jan 9:1-9. Epub 2021 Jan 9.

Center for Drug Design, College of Pharmacy, University of Minnesota, Minneapolis, MN 55455 USA.

The AAA+ (ATPase associated with various cellular activities) protein p97, also called valosin-containing protein, is a hexameric ring ATPase and uses ATP hydrolysis to unfold or extract proteins from biological complexes. Many cellular processes are affected by p97 including ER-associated degradation, DNA damage response, cell signaling (NF-κB), cell cycle progression, autophagy, and others. Not surprisingly, with its role in many fundamental cellular processes, p97 function is important for the replication of many viruses. We tested irreversible p97-targeting compounds for their ability to inhibit the replication of multiple viruses compared to the known p97 inhibitors NMS-873 and CB-5083. Our results indicate that overall cellular toxicity for p97 compounds provides a challenge for antivirals targeting p97. However, we identified one compound with sub-micromolar activity against human cytomegalovirus and improved cell viability to provide evidence for the potential of irreversible p97 inhibitors as antivirals.
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http://dx.doi.org/10.1007/s00044-020-02679-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7794631PMC
January 2021

Construction of Zeolite-Loaded Fluorescent Supramolecular on-off Probes for Corrosion Detection Based on a Cation Exchange Mechanism.

Nanomaterials (Basel) 2021 Jan 11;11(1). Epub 2021 Jan 11.

School of Ocean, Yantai University, Yantai 264005, China.

Metal engineering structures are commonly covered and protected by coatings. However, the early local corrosion under the coatings and at defects is difficult to detect and discover. Visibility to the naked eye means that corrosion has already developed and expanded. Therefore, it is practical significant to detect the early corrosion of coated metal. Based on the formation of iron ions and anodic acidification in the local corrosion process, iron ions and proton responsive fluorescent rhodamine B acylhydrazone on-off probes are prepared by newly improved methods and denoted as RBA. RBA are loaded on the surface and in the lattice cage of zeolite (ZEO) to protect RBA from premature exposure to the corrosive environment and fluorescence quenching. In corrosive environments, the RBA loaded on the surface are released and complex with iron ions in the environment to activate fluorescence characteristics. Simultaneously, due to the cation exchange of ZEO, iron ions enter the lattice cage of ZEO and combine with RBA in the lattice cage to turn on fluorescence. When applied in epoxy coatings, the RBA/ZEO effectively indicate the occurrence of corrosion under the coatings and at defects, and accurately locate the corrosion site. Nano-scale ZEO (or RBA/ZEO) fill the micropores such as pinholes and defects of the coatings, and increase the difficulty of diffusion and penetration of corrosive media into the coatings. The application of RBA/ZEO functional filler not only do not weaken the main anti-corrosion performance of the coatings, but also significantly improve it.
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http://dx.doi.org/10.3390/nano11010169DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7826598PMC
January 2021

SENP3 senses oxidative stress to facilitate STING-dependent dendritic cell antitumor function.

Mol Cell 2021 03;81(5):940-952.e5

Shanghai Institute of Immunology, Department of Immunology and Microbiology, State Key Laboratory of Oncogenes and Related Genes, Shanghai Jiao Tong University School of Medicine, 280 South Chongqing Road, Shanghai 200025, China. Electronic address:

STING-dependent cytosolic DNA sensing in dendritic cells (DCs) initiates antitumor immune responses, but how STING signaling is metabolically regulated in the tumor microenvironment remains unknown. Here, we show that oxidative stress is required for STING-induced DC antitumor function through a process that directs SUMO-specific protease 3 (SENP3) activity. DC-specific deletion of Senp3 drives tumor progression by blunting STING-dependent type-I interferon (IFN) signaling in DCs and dampening antitumor immune responses. DC-derived reactive oxygen species (ROS) trigger SENP3 accumulation and the SENP3-IFI204 interaction, thereby catalyzing IFI204 deSUMOylation and boosting STING signaling activation in mice. Consistently, SENP3 senses ROS to facilitate STING-dependent DC activity in tissue samples from colorectal cancer patients. Our results reveal that oxidative stress as a metabolic regulator promotes STING-mediated DC antitumor immune responses and highlights SENP3 as an overflow valve for STING signaling induction in the metabolically abnormal tumor microenvironment.
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http://dx.doi.org/10.1016/j.molcel.2020.12.024DOI Listing
March 2021

Expression and Prognostic Significance of Metastasis-Associated Protein 1 in Gastrointestinal Cancer.

Front Oncol 2020 21;10:542330. Epub 2020 Dec 21.

Department of General Surgery, First Affiliated Hospital of Anhui Medical University, Hefei, China.

Background: Metastasis-associated protein 1 (MTA1) has been considered as a transcriptional regulator, which is significantly related to the prognosis in various types of tumors. However, whether MTA1 is a potential prognostic index of gastrointestinal cancer (GIC) remains controversial. The current meta-analysis was performed to evaluate the role of MTA1 expression in the prediction of the clinicopathological features and survival in GIC cases. And the results of gastric cancer were verified by immunohistochemistry (IHC).

Methods: Eligible studies assessing the relationship between MTA1 and GIC by IHC were searched in the PubMed, Cochrane, Ovid, Web of Science and CNKI databases by various search strategies. The STATA 16.0 software was applied to gather data and to analyze the potential relationship between MTA1 and GIC. The expression level of MTA1 was examined in 80 GC samples by IHC assay. SPSS 20.0 was applied for statistical analysis, and the survival curves were calculated by the Kaplan-Meier method. The data of 95% CI was displayed as "[a-b]".

Results: According to the meta-analysis, the expression level of MTA1 was tightly associated with the tumor size (OR=1.82 [1.16-2.84], =0.009), tumor tissue differentiation (OR=1.71 [1.24-2.37], =0.001), depth of invasion (OR=3.12 [2.55-3.83], 0.001), lymphatic metastasis (OR=2.99 [2.02-4.43], <0.001), distant metastasis (OR=4.66 [1.13-19.24], 0.034), TNM stage (OR=4.28 [2.76-6.63], <0.001). In addition, MTA1 played the negative effects in 1- (RR=2.48 [1.45-4.25], =0.001), 3- (RR=1.66 [1.30-2.11], <0.001) and 5-year (RR=1.73 [1.37-2.20], <0.001). Study in subgroup, grouped by language and tumor type, we reached similar conclusions. Further validation by IHC yielded similar conclusions. Tumor size (=0.008), lymph node metastasis (=0.007) and distant metastasis (=0.023) significantly accompanied with higher expression of MAT1 in GC cases. Besides, the expression level of MTA1 was statistically significantly correlated with OS in GC cases (HR=2.061 [1.066-3.986], =0.032), which suggested that MTA1 might be an independent prognostic marker for GC. Finally, we verified the correlation between the expression level of MTA1 and prognosis of GC in 80 GC samples.

Conclusions: MTA1 is tightly associated with metastasis-related factors and may constitute a promising prognostic factor of GIC.
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http://dx.doi.org/10.3389/fonc.2020.542330DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7780747PMC
December 2020

Meet-in-metabolite analysis: A novel strategy to identify connections between arsenic exposure and male infertility.

Environ Int 2021 Feb 2;147:106360. Epub 2021 Jan 2.

State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, PR China; Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, PR China. Electronic address:

Background: Despite a trend in the use of systems epidemiology to fill the knowledge gap between risk-factor exposure and adverse outcomes in the OMICS data, such as the metabolome, seriously hindrances need to be overcome for identifying molecular connections.

Objectives: Using male infertility phenotypes and arsenic exposure, we aimed to identify intermediate biomarkers that reflect both arsenic exposure and male infertility with a meet-in-metabolite analysis (MIMA).

Methods: Urinary arsenic levels and metabolome were measured by using inductively coupled plasma-mass spectrometry (ICP-MS) and HPLC-quadrupole time-of-flight mass spectrometry (HPLC-QTOF-MS), respectively. To identify arsenic-related metabolic markers (A-MIMA), the intermediate markers were profiled by orthogonal projections to latent structures (OPLS-DA). To detect infertility-related metabolic markers (I-MIMA), the intermediate markers were investigated by weighted gene co-expression network analysis. The key node markers, related to both A-MIMA and I-MIMA, were determined by O2PLS and defined as MIMA markers. Finally, network analysis was used to construct the MIMA-related metabolic network.

Results: Twelve markers each were defined through the significant associations with arsenic exposure (A-MIMA) and/or infertility (I-MIMA), respectively. Seven of them, including acetyl-N-formyl-5-methoxykynurenamine, carnitine, estrone, 2-oxo-4-methylthiobutanoic acid, malonic acid, valine, and LysoPC (10:0), were defined through the associations with both arsenic exposure and male infertility (MIMA markers). These intermediate markers were involved majorly in oxidative stress, one-carbon metabolism, steroid hormone homeostasis, and lipid metabolism pathways. The core correlation network analysis further highlighted that testosterone is a vital link between the effect of arsenic and male infertility.

Conclusions: From arsenic exposure to male infertility, the arsenic methylation that coupled one-carbon metabolism disruption with oxidation stress may have extended its effect to fatty acid oxidation and steroidogenesis dysfunction. Testosterone is at the hub between arsenic exposure and male infertility modules and, along with the related metabolic pathways, may service as a potential surrogate marker in risk assessment for male dysfunction due to arsenic exposure.
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http://dx.doi.org/10.1016/j.envint.2020.106360DOI Listing
February 2021

Long non-coding RNA MEG3 silencing and microRNA-214 restoration elevate osteoprotegerin expression to ameliorate osteoporosis by limiting TXNIP.

J Cell Mol Med 2021 Feb 3;25(4):2025-2039. Epub 2021 Jan 3.

Division of Spine Surgery, Section II, Department of Orthopedics, The Third Affiliated Hospital of Southern Medical University, Guangdong Provincial Key Laboratory of Bone and Joint Degeneration Diseases, Southern Medical University, Academy of Orthopedics of Guangdong Province, Guangzhou, China.

Studies have shown that long non-coding RNA (lncRNA) MEG3 plays a key role in osteoporosis (OP), but its regulatory mechanism is somewhat incompletely clear. Here, we intend to probe into the mechanism of MEG3 on OP development by modulating microRNA-214 (miR-214) and thioredoxin-interacting protein (TXNIP). Rat models of OP were established. MEG3, miR-214 and TXNIP mRNA expression in rat femoral tissues were detected, along with TXNIP, OPG and RANKL protein expression. BMD, BV/TV, Tb.N and Tb.Th in tissue samples were measured. Ca, P and ALP contents in rat serum were also determined. Primary osteoblasts were isolated and cultured. Viability, COL-I, COL-II and COL-Χ mRNA expression, PCNA, cyclin D1, OCN, RUNX2 and osteolix protein expresion, ALP content and activity, and mineralized nodule area of rat osteoblasts were further detected. Dual-luciferase reporter gene and RNA-pull down assays verified the targeting relationship between MEG3, miR-214 and TXNIP. MEG3 and TXNIP were up-regulated while miR-214 was down-regulated in femoral tissues of OP rats. MEG3 silencing and miR-214 overexpression increased BMD, BV/TV, Tb.N, Tb.Th, trabecular bone area, collagen area and OPG expression, and down-regulated RANKL of femoral tissues in OP rats. MEG3 silencing and miR-214 overexpression elevated Ca and P and reduced ALP in OP rat serum, elevated osteoblast viability, differentiation ability, COL-I and COL-Χ expression and ALP activity, and reduced COL-II expression of osteoblasts. MEG3 specifically bound to miR-214 to regulate TXNIP. MEG3 silencing and miR-214 overexpression promote proliferation and differentiation of osteoblasts in OP by down-regulating TXNIP, which further improves OP.
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http://dx.doi.org/10.1111/jcmm.16096DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7882928PMC
February 2021

What Are the Risk Factors for Surgical Site Infection in HIV-Positive Patients Receiving Open Reduction and Internal Fixation of Traumatic Limb Fractures? A Retrospective Cohort Study.

AIDS Res Hum Retroviruses 2021 Jan 27. Epub 2021 Jan 27.

Department of Orthopedic, Beijing Ditan Hospital, Capital Medical University, Beijing, China.

A lack of studies analyze risk factors associated with surgical site infection (SSI) in human immunodeficiency virus (HIV)-positive patients with trauma undergoing orthopedic surgery. We questioned, (1) "What is the proportion of SSI in HIV-positive patients receiving open reduction and internal fixation (ORIF) of traumatic limb fractures?" and (2) "What are the independent risk factors for SSI of HIV-positive patients with traumatic limb fractures who received ORIF?" A retrospective study was performed in our institution from May 2011 to December 2019. A total of 246 patients were enrolled. Data of HIV-positive adult patients with traumatic limb fractures treated by ORIF were extracted from the electronic medical records, including the demographic information, characteristics of fractures, treatment-related variables, and indexes of laboratory examination. Multivariable logistic regression was used to identify independent predictors of SSI. A total of 26 patients developed SSI in this study, the overall proportion was 10.6% [95% confidence interval (CI) = 7.2%-15.3%]. Among them, 18 cases (7.3%) suffered from superficial SSI and 8 cases (3.3%) had the deep SSI. Independent predictors of SSI identified by multivariable logistic regression analysis were CD4 T-lymphocyte count (adjusted odds ratio per 100/μL increase = 0.55; 95% CI = 0.37-0.81;  = .002) and albumin (adjusted odds ratio per 5 g/liter increase = 0.50; 95% CI = 0.30-0.81;  = .003). We recommend that orthopedic surgeons assess immunosuppression and nutritional status of HIV-positive patients rigorously and optimize the perioperative strategy of supplementary to reduce the risk of SSI.
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http://dx.doi.org/10.1089/AID.2020.0184DOI Listing
January 2021

The Association between Vitamin D Status and Autism Spectrum Disorder (ASD): A Systematic Review and Meta-Analysis.

Nutrients 2020 Dec 29;13(1). Epub 2020 Dec 29.

Department of Biomedical Informatics, School of Basic Medical Sciences, Peking University, Beijing 100191, China.

The association between vitamin D status and autism spectrum disorder (ASD) is well-investigated but remains to be elucidated. We quantitatively combined relevant studies to estimate whether vitamin D status was related to ASD in this work. PubMed, EMBASE, Web of Science, and the Cochrane Library were searched to include eligible studies. A random-effects model was applied to pool overall estimates of vitamin D concentration or odds ratio (OR) for ASD. In total, 34 publications involving 20,580 participants were identified in this present study. Meta-analysis of 24 case-control studies demonstrated that children and adolescents with ASD had significantly lower vitamin D concentration than that of the control group (mean difference (MD): -7.46 ng/mL, 95% confidence interval (CI): -10.26; -4.66 ng/mL, < 0.0001, I = 98%). Quantitative integration of 10 case-control studies reporting OR revealed that lower vitamin D was associated with higher risk of ASD (OR: 5.23, 95% CI: 3.13; 8.73, < 0.0001, I = 78.2%). Analysis of 15 case-control studies barring data from previous meta-analysis reached a similar result with that of the meta-analysis of 24 case-control studies (MD: -6.2, 95% CI: -9.62; -2.78, = 0.0004, I = 96.8%), which confirmed the association. Furthermore, meta-analysis of maternal and neonatal vitamin D showed a trend of decreased early-life vitamin D concentration in the ASD group (MD: -3.15, 95% CI: -6.57; 0.26, = 0.07, I = 99%). Meta-analysis of prospective studies suggested that children with reduced maternal or neonatal vitamin D had 54% higher likelihood of developing ASD (OR: 1.54, 95% CI: 1.12; 2.10, = 0.0071, I = 81.2%). These analyses indicated that vitamin D status was related to the risk of ASD. The detection and appropriate intervention of vitamin D deficiency in ASD patients and pregnant and lactating women have clinical and public significance.
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http://dx.doi.org/10.3390/nu13010086DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7824115PMC
December 2020

The complete chloroplast genome sequence of the threatened (Orchidaceae).

Mitochondrial DNA B Resour 2019 Nov 22;4(2):4220-4222. Epub 2019 Nov 22.

Institute of Floriculture, Liaoning Academy of Agricultural Sciences, Shenyang, China.

The complete chloroplast genome of , a rare species in the family Orchidaceae was reported in this study. The genome size is 175,122 bp in length, and contains four sub-regions: 97,486 bp of large single copy (LSC) and 22,260 bp of small copy (SSC) regions, separated by 27,688 bp of inverted repeat (IR) regions. A total of 133 genes were annotated, including 87 protein-coding genes, 38 tRNA genes and 8 rRNA genes. The GC content of this cp genome is 34.36%. Phylogenetic analysis revealed a close relationship between with and . This is the first complete cp genome for that would be useful for conservation and phylogenetic studies of this species.
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http://dx.doi.org/10.1080/23802359.2019.1693933DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7707734PMC
November 2019

ZFP91 is required for the maintenance of regulatory T cell homeostasis and function.

J Exp Med 2021 Feb;218(2)

Shanghai Institute of Immunology, Department of Immunology and Microbiology, State Key Laboratory of Oncogenes and Related Genes, Hongqiao International Institute of Medicine, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Autophagy programs the metabolic and functional fitness of regulatory T (T reg) cells to establish immune tolerance, yet the mechanisms governing autophagy initiation in T reg cells remain unclear. Here, we show that the E3 ubiquitin ligase ZFP91 facilitates autophagy activation to sustain T reg cell metabolic programming and functional integrity. T reg cell-specific deletion of Zfp91 caused T reg cell dysfunction and exacerbated colonic inflammation and inflammation-driven colon carcinogenesis. TCR-triggered autophagy induction largely relied on T reg cell-derived ZFP91 to restrict hyperglycolysis, which is required for the maintenance of T reg cell homeostasis. Mechanistically, ZFP91 rapidly translocated from the nucleus to the cytoplasm in response to TCR stimulation and then mediated BECN1 ubiquitination to promote BECN1-PIK3C3 complex formation. Therefore, our results highlight a ZFP91-dependent mechanism promoting TCR-initiated autophagosome maturation to maintain T reg cell homeostasis and function.
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http://dx.doi.org/10.1084/jem.20201217DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7769166PMC
February 2021

High Plasticity of the Gut Microbiome and Muscle Metabolome of Chinese Mitten Crab () in Diverse Environments.

J Microbiol Biotechnol 2021 Feb;31(2):240-249

Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture/National Demonstration Center for Experimental Fisheries Science Education/Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, P.R. China.

Phenotypic plasticity is a rapid response mechanism that enables organisms to acclimate and survive in changing environments. The Chinese mitten crab () survives and thrives in different and even introduced habitats, thereby indicating its high phenotypic plasticity. However, the underpinnings of the high plasticity of have not been comprehensively investigated. In this study, we conducted an integrated gut microbiome and muscle metabolome analysis on collected from three different environments, namely, an artificial pond, Yangcheng Lake, and Yangtze River, to uncover the mechanism of its high phenotypic plasticity. Our study presents three divergent gut microbiotas and muscle metabolic profiles that corresponded to the three environments. The composition and diversity of the core gut microbiota (Proteobacteria, Bacteroidetes, Tenericutes, and Firmicutes) varied among the different environments while the metabolites associated with amino acids, fatty acids, and terpene compounds displayed significantly different concentration levels. The results revealed that the gut microbiome community and muscle metabolome were significantly affected by the habitat environments. Our findings indicate the high phenotypic plasticity in terms of gut microbiome and muscle metabolome of when it faces environmental changes, which would also facilitate its acclimation and adaptation to diverse and even introduced environments.
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http://dx.doi.org/10.4014/jmb.2011.11018DOI Listing
February 2021

Associations between weekly air pollution exposure and congenital heart disease.

Sci Total Environ 2021 Feb 18;757:143821. Epub 2020 Nov 18.

Department of Teaching Center for Preventive Medicine, School of Public Health, Anhui Medical University, 81 Meishan Road, Hefei, Anhui 230032, China. Electronic address:

Background: The topic of congenital heart diseases (CHDs) has attracted more and more attentions. Accumulating evidence suggests that exposure to air pollutants during pregnancy is associated with CHDs, yet the results are inconsistent and study about weekly exposure is few. Our study evaluated the association between weekly air pollution and CHDs in Hefei, China.

Materials And Methods: Daily CHDs admission data were obtained from eight hospitals in Hefei from October 2015 to September 2017. Meteorological data and air quality were collected from China Meteorological Data Network. Distributed lag nonlinear model (DLNM) considering both the lag effect of exposure factors and the nonlinear relationship of exposure-reaction was used to assess the effect of weekly air pollutants exposure on CHDs admission.

Results: During the study period, totally 47,046 cases of perinatal infants were recruited, and the incidence of CHDs was 9.71 per thousand. The findings showed PM, PM, SO and NO significantly increased the risk of CHDs. Each 10 μg/m increase in PM during gestational weeks 20-26 increased the risk of CHDs. The susceptibility windows of PM (weeks 0-2 and weeks 25-29 of pregnancy), SO (weeks 8-16 and weeks 29-38) and NO (week 40), while the strongest effects of these 4 pollutants on CHDs were observed in week 22 (RR = 1.034, 95% CI: 1.007-1.062), week 0 (RR = 1.081, 95% CI: 1.02-1.146), week 37 (RR = 1.528, 95% CI: 1.085-2.153) and week 40 (RR = 1.171, 95% CI: 1.006-1.364), respectively.

Conclusions: Air pollutants (SO, NO, PM, and PM) exposure could increase the risk of CHDs, while the most crucial susceptibility windows for the exposure were mainly in the second and third trimesters. Boys seemed to be more sensitive to air pollution. Our study contributes to the knowledge of the association between maternal exposure to air pollution and CHDs, but the associations need to be verified by further studies.
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http://dx.doi.org/10.1016/j.scitotenv.2020.143821DOI Listing
February 2021

Comparison of Retrograde Intrarenal Surgery and Micro-Percutaneous Nephrolithotomy for Kidney Stones: A Meta-Analysis.

Urol Int 2021 23;105(1-2):64-70. Epub 2020 Nov 23.

Department of Urology, Wuxi Traditional Chinese Medicine Hospital, Wuxi, China,

Background: Advances in micro-percutaneous nephrolithotomy (PCNL) for kidney stones have made it an alternative approach to the retrograde intrarenal surgery (RIRS) approach. Nevertheless, the superiority of micro-PCNL over RIRS is still under debate. The results are controversial.

Objectives: The purpose of this study was to systematically evaluate the clinical results in patients presenting with kidney stones treated with micro-PCNL or RIRS.

Methods: A literature search was done for electronic databases to identify researches that compared micro-PCNL and RIRS till December 2019. The clinical outcome included complications, stone-free rates (SFRs), hemoglobin reduction, length of hospital stay, and operative time.

Results: Five articles were included in our study. The pooled results revealed no statistical difference in the rate of complications (OR = 0.99, 95% CI = 0.57-1.74, p = 0.99), length of hospital stay (MD = -0.29, 95% CI = -0.82 to 0.24, p = 0.28), and operative time (MD = -6.63, 95% CI = -27.34 to 14.08, p = 0.53) between the 2 groups. However, significant difference was present in hemoglobin reduction (MD = -0.43, 95% CI = -0.55 to 0.30, p < 0.001) and the SFRs (OR = 0.59, 95% CI = 0.36-0.98, p = 0.04) when comparing RIRS with micro-PCNL.

Conclusions: Compared with micro-PCNL to treat kidney stones, RIRS is associated with better stone clearance and bearing higher hemoglobin loss. As the advantages of both technologies have been shown in some fields, the continuation of well-designed clinical trials may be necessary.
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http://dx.doi.org/10.1159/000506716DOI Listing
November 2020

Comprehensive evaluation of differential long non-coding RNA and gene expression in patients with cartilaginous endplate degeneration of cervical vertebra.

Exp Ther Med 2020 Dec 27;20(6):260. Epub 2020 Oct 27.

Department of Orthopedics, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.

Long non-coding RNAs (lncRNAs) are emerging as key regulators in gene expression; however, little is currently known regarding their role in cartilaginous endplate (CE) degeneration (CED) of cervical vertebra. The present study aimed to investigate the expression levels of lncRNAs and analyze their potential functions in CED of cervical vertebra in patients with cervical fracture and cervical spondylosis. Human competitive endogenous RNA (ceRNA) array was used to analyze lncRNA and mRNA expression levels in CE samples from patients with cervical fracture and cervical spondylosis, who received anterior cervical discectomy and fusion. Differentially expressed lncRNAs (DELs) or differentially expressed genes (DEGs) were identified and functionally analyzed, using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. An lncRNA-microRNA(miRNA)-mRNA ceRNA regulatory network was constructed based on the DELs and DEGs, and the ceRNA network was visualized using Cytoscape 3.7.2 software. In total, one downregulated mRNA, one upregulated miRNA and five downstream regulated lncRNAs were identified using reverse transcription-quantitative PCR in CED and healthy CE samples. A total of 369 lncRNAs and 246 mRNAs were identified as differentially expressed in CE. The GO and KEGG analyses demonstrated that the majority of GO and KEGG enrichments were associated with CED. Furthermore, a ceRNA network was established, including 168 putative miRNA response elements, 189 upregulated and 37 downregulated lncRNAs and 47 upregulated and 10dow regulated DEGs. The present study analyzed the function of DEGs in the ceRNA network and filtered out the same items as in DEG-function enrichment analysis. These results provide a new perspective for an improved understanding of ceRNA-mediated gene regulation in cervical spondylosis, and provide a novel theoretical basis for further studies on the function of lncRNA in cervical spondylosis. However, further experiments are required to validate the results of the present study.
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http://dx.doi.org/10.3892/etm.2020.9390DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7664616PMC
December 2020

GPR173 agonist phoenixin 20 promotes osteoblastic differentiation of MC3T3-E1 cells.

Aging (Albany NY) 2020 11 10;13(4):4976-4985. Epub 2020 Nov 10.

Division of Spine Surgery, Section II, Department of Orthopedics, Academy of Orthopedics of Guangdong Province, The Third Affiliated Hospital of Southern Medical University, Guangzhou, Guangdong Province, China.

Osteogenic differentiation is critical to bone homeostasis, and its imbalance plays a key role in the progression of osteoporosis. Osteoblast cells are responsible for synthesizing new bone tissue, and understanding how to control osteoblastic differentiation is vital to the treatment of osteoporosis. Herein, we show that GPR173 signaling is involved in the regulation of osteoblastic differentiation in MC3T3-E1 cells. Our data reveals that GPR173 is abundantly expressed in MC3T3-E1 cells, and its expression is inducible upon the introduction of osteogenic media. The activation of GPR173 by its selective agonist phoenixin 20 induces the expression of several osteoblast signature genes including collagen type 1 alpha 1 (Col-I), osteocalcin (OCN), alkaline phosphatase (ALP) as well as increased matrix mineralization and ALP activity, suggesting that the activation of GPR173 promotes osteoblastic differentiation. Moreover, we show that the effect of phoenixin 20 is mediated by its induction on the key regulator runt-Related Transcription Factor 2 (Runx2). Mechanistically, we display that the action of phoenixin 20 requires the activation of MAPK kinase p38, and deactivation of p38 by its inhibitor SB203580 weakens the phoenixin 20-mediated induction of RUNX-2, ALP, and matrix mineralization. Silencing of GPR173 attenuates phoenixin 20-mediated osteoblastic differentiation, indicating its dependence on the receptor. Collectively, our study reveals a new role of GPR173 and its agonist phoenixin 20 in osteoblastic differentiation.
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http://dx.doi.org/10.18632/aging.103717DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7950309PMC
November 2020

A quasi-paired cohort strategy reveals the impaired detoxifying function of microbes in the gut of autistic children.

Sci Adv 2020 Oct 21;6(43). Epub 2020 Oct 21.

Department of Biomedical Informatics, School of Basic Medical Sciences, Peking University, Beijing 100191, China.

Growing evidence suggests that autism spectrum disorder (ASD) is strongly associated with dysbiosis in the gut microbiome, with the exact mechanisms still unclear. We have proposed a novel analytic strategy-quasi-paired cohort-and applied it to a metagenomic study of the ASD microbiome. By comparing paired samples of ASD and neurotypical subjects, we have identified significant deficiencies in ASD children in detoxifying enzymes and pathways, which show a strong correlation with biomarkers of mitochondrial dysfunction. Diagnostic models based on these detoxifying enzymes accurately distinguished ASD individuals from controls, and the dysfunction score inferred from the model increased with the clinical rating scores of ASD. In summary, our results suggest a previously undiscovered potential role of impaired intestinal microbial detoxification in toxin accumulation and mitochondrial dysfunction, a core component of ASD pathogenesis. These findings pave the way for designing future therapeutic strategies to restore microbial detoxification capabilities for patients with ASD.
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http://dx.doi.org/10.1126/sciadv.aba3760DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7577716PMC
October 2020

Safety and efficacy of polyetheretherketone (PEEK) cages in combination with one-stage posterior debridement and instrumentation in Lumbar Brucella Spondylitis.

Clin Neurol Neurosurg 2020 Dec 30;199:106259. Epub 2020 Sep 30.

Department of Orthopedic, Beijing Ditan Hospital, Capital Medical University, Beijing, 100015, China. Electronic address:

Objective: The aim of this study was to explore the efficacy and safety of surgical treatment of lumbar brucella spondylitis with PEEK cages combined with one-stage posterior debridement and instrumentation.

Methods: We performed a retrospective study for adult patients with lumbar brucella spondylitis. Medical records, imaging studies and laboratory data were collected, back pain was measured by employing the visual analog scale (VAS) and the neurological status was evaluated by using the American Spinal Injury Association (ASIA) scale.

Results: A total of 61 consecutive patients (42 males and 19 females) were enrolled with the mean age at presentation of 56.33 ± 9.16 years old. L3-4 and L4-5 were the most infected levels with the ratios of 21.31 % and 19.67 % respectively. Moreover, 12 (19.67 %) patients suffered multiple-level infection and 5 (5/12, 41.67 %) of them had non-contiguous spondylitis. In addition, epidural masses were found in 26 (42.62 %) cases and psoas abscesses were found in 14 (22.95 %) cases. The number of operative segment depended on operation indications (failure of conservative measures, instability, kyphosis, intractable pain and/or neurological impairment). VAS scores were significantly improved at 6-weeks and the last follow-up. Among the 23 (37.70 %) patients with neurological deficits, 19 (19/23, 82.61 %) obtained a full recovery and 4 (4/23, 17.39 %) had been improved incompletely during the last follow-up. All patients exhibited satisfactory bone fusion during the last follow-up. Local infection of surgical site was identified in 6 (9.84 %) cases and no other surgery-related complications were found.

Conclusion: Use of PEEK cages for interbody fusion is feasible and safe in patients suffering from lumbar brucella spondylitis.
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http://dx.doi.org/10.1016/j.clineuro.2020.106259DOI Listing
December 2020