Publications by authors named "Rui Chen"

1,847 Publications

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KCNQ1OT1 lncRNA affects the proliferation, apoptosis, and chemoresistance of small cell lung cancer cells via the JAK2/STAT3 axis.

Ann Transl Med 2021 May;9(10):891

Department of Pathology, Guangdong Provincial Corps Hospital of Chinese People's Armed Police Forces, Guangzhou Medical University, Guangzhou, China.

Background: Small cell lung cancer (SCLC) is a devastating and aggressive neuroendocrine carcinoma characterized by high cellular proliferation and early metastatic spread. Numerous studies have demonstrated that long noncoding RNAs (lncRNAs) can regulate tumor generation and development, including in SCLC. The current study aimed to assess the effect of the lncRNA, KCNQ1OT1, on the proliferation, apoptosis, and chemoresistance of SCLC and the potential underlying molecular mechanism.

Methods: Matched chemo-resistant and sensitive cells were applied to RNA isolation and followed by expression profiling by microarray analysis and subsequent quantitative polymerase chain reaction (qPCR) validation. Cell viability and apoptosis were determined by Cell Counting Kit-8 and flow cytometry to examine the chemoresistance and apoptosis of KCNQ1OT1 knockdown with lentivirus-mediated RNA interference. Furthermore, cell proliferation was studied by colony formation, and invasion and migration were tested by Transwell cell invasion and wound-healing assays, respectively. A tumor xenograft model was established to determine the role of KCNQ1OT1 in tumor growth and chemoresistance in response to KCNQ1OT1 knockdown . Western blot analysis, qPCR, and immunohistochemistry were used to detect the levels of messenger RNA (mRNA) Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3) pathway-related markers.

Results: Higher expression of KCNQ1OT1 was detected in SCLC chemo-resistant verso chemo-sensitive cells. Knockdown of KCNQ1OT1 inhibited SCLC cell viability and cloning ability, hindered cell migration and invasion, induced apoptosis , and suppressed tumor growth and chemoresistance , by activating the JAK2/STAT3 signaling pathway.

Conclusions: This is the first study to indicate that lncRNA KCNQ1OT1 promotes cell proliferation and invasion, and prevents apoptosis of SCLC by activating the JAK2/STAT3 pathway.
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http://dx.doi.org/10.21037/atm-21-1761DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184448PMC
May 2021

Construction of a Prognostic Nomogram for Colorectal Cancer Patients with Fewer than Twelve Lymph Nodes Examined: a Population-Based Study in the SEER Database and China.

J Gastrointest Surg 2021 Jun 22. Epub 2021 Jun 22.

Department of Colorectal Surgery, Cancer Hospital of University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, Hangzhou, China.

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http://dx.doi.org/10.1007/s11605-021-05060-8DOI Listing
June 2021

Two effective clinical prediction models to screen for obstructive sleep apnoea based on body mass index and other parameters.

Sleep Breath 2021 Jun 17. Epub 2021 Jun 17.

Department of Respiratory Medicine, The Second Affiliated Hospital of Soochow University, Soochow University, No. 1055, Sanxiang Road, Suzhou, 215004, Jiangsu, China.

Background And Objective: The diagnosis of obstructive sleep apnea (OSA) relies on polysomnography which is time-consuming and expensive. We therefore aimed to develop two simple, non-invasive models to screen adults for OSA.

Methods: The effectiveness of using body mass index (BMI) and a new visual prediction model to screen for OSA was evaluated using a development set (1769 participants) and confirmed using an independent validation set (642 participants).

Results: Based on the development set, the best BMI cut-off value for diagnosing OSA was 26.45 kg/m, with an area under the curve (AUC) of 0.7213 (95% confidence interval (CI), 0.6861-0.7566), a sensitivity of 57% and a specificity of 78%. Through forward conditional logistic regression analysis using a stepwise selection model developed from observed data, seven clinical variables were evaluated as independent predictors of OSA: age, BMI, sex, Epworth Sleepiness Scale score, witnessed apnoeas, dry mouth and arrhythmias. With this new model, the AUC was 0.7991 (95% CI, 0.7668-0.8314) for diagnosing OSA (sensitivity, 75%; specificity, 71%). The results were confirmed using the validation set. A nomogram for predicting OSA was generated based on this new model using statistical software.

Conclusions: BMI can be used as an indicator to screen for OSA in the community. We created an internally validated, highly distinguishable, visual and parsimonious prediction model comprising BMI and other parameters that can be used to identify patients with OSA among outpatients. Use of this prediction model may help to improve clinical decision-making.
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http://dx.doi.org/10.1007/s11325-021-02347-7DOI Listing
June 2021

An improved ultrasonic coda wave method for concrete behavior monitoring under various loading conditions.

Ultrasonics 2021 Jun 8;116:106498. Epub 2021 Jun 8.

College of Automotive and Mechanical Engineering, Changsha University of Science and Technology, Changsha, Hunan 410114, China.

Monitoring of concrete behavior is an important task to evaluate the safety of concrete structures. This paper proposes a new stretching factor accumulation method based on the stepwise coda wave interference (CWI) to accurately calculate the relative velocity change Δv/v. The Δv/v and residual decorrelation coefficient K are used to study the concrete behavior in step loading experiment and short-time fixed loading experiment. The results show that: (1) In the loading stage of step loading experiments, both Δv/v and K can be used to monitor the load and the microcrack development; (2) In the relaxation stage of step loading experiments, the slow dynamics effect of concrete specimens can be characterized by Δv/v and K; (3) In the short-time fixed loading experiments, both Δv/v and K increase with the time of fixed loading, and the increasing rate of Δv/v decreases with the increase of concrete strength. This work can be used to promote the development of ultrasonic testing techniques that aid in concrete behavior monitoring.
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http://dx.doi.org/10.1016/j.ultras.2021.106498DOI Listing
June 2021

Optogenetic control of calcium influx in mammalian cells.

Methods Enzymol 2021 16;654:255-270. Epub 2021 Mar 16.

Center for Translational Cancer Research, Institute of Biosciences and Technology, Texas A&M University, Houston, TX, United States. Electronic address:

Optogenetics combines optics and genetics to enable non-invasive interrogation of cell physiology at an unprecedented high spatiotemporal resolution. Here, we introduce Opto-CRAC as a set of genetically-encoded calcium actuators (GECAs) engineered from the calcium release-activated calcium (CRAC) channel, which has been tailored for optical control of calcium entry and calcium-dependent physiological responses in non-excitable cells and tissues. We describe a detailed protocol for applying Opto-CRAC as an optogenetic tool to achieve photo-tunable control over intracellular calcium signals and calcium-dependent gene expression in mammalian cells.
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http://dx.doi.org/10.1016/bs.mie.2021.02.010DOI Listing
March 2021

CIRBP promotes ferroptosis by interacting with ELAVL1 and activating ferritinophagy during renal ischaemia-reperfusion injury.

J Cell Mol Med 2021 Jun 10. Epub 2021 Jun 10.

Department of Organ Transplantation, Shanghai Changhai Hospital, Shanghai, China.

Renal ischaemia-reperfusion (IR) is a major cause of acute kidney injury (AKI). Cold-inducible RNA-binding protein (CIRBP) may contribute to AKI because its deficiency protects against renal IR injury in a mechanism believed to involve ferroptosis. We aimed to investigate whether ferroptosis is associated with CIRBP-mediated renal damage. The differential expression of CIRBP was examined in tubular epithelial (HK2) cells during hypoxia-reoxygenation (HR) or in response to erastin, an inducer of ferroptosis. CIRBP expression was increased in response to HR or erastin in HK2 cells but the silencing of CIRBP inhibited HR and erastin-induced ferroptosis together with ferritinophagy. We discovered an interaction between CIRBP and ELAVL1 using STRING software, which was verified through co-immunoprecipitation and fluorescence colocalization assays. We found that ELAVL1 is a critical regulator in the activation of ferritinophagy and the promotion of ferroptosis. HR or erastin also induced the expression of ELAVL1. An autophagy inhibitor (hydroxychloroquine) or si-ELAVL1 transfection reversed CIRBP-enhanced ferritinophagy activation and ferroptosis in HK2 cells under HR. Injection of anti-CIRBP antibody into a mouse model of IR inhibited ferroptosis and decreased renal IR injury in vivo. In summary, our results provide evidence that ferritinophagy-mediated ferroptosis could be responsible for CIRBP-enhanced renal IR injury.
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http://dx.doi.org/10.1111/jcmm.16567DOI Listing
June 2021

Advanced Retinal Imaging and Ocular Parameters of the Rhesus Macaque Eye.

Transl Vis Sci Technol 2021 May;10(6)

Department of Ophthalmology & Vision Science, School of Medicine, University of California-Davis, Sacramento, CA, USA.

Purpose: To determine the range of normal ocular biometry and perform advanced retinal imaging and functional assessment of the rhesus macaque eye.

Methods: We performed ocular phenotyping on rhesus macaques at the California National Primate Research Center. This process consisted of anterior and posterior segment eye examination by ophthalmologists, advanced retinal imaging, and functional retinal electrophysiology.

Results: Full eye examinations were performed on 142 animals, consisting of pupillary light reflex, tonometry, external examination and photography, anterior slit lamp examination, and posterior segment examination by indirect ophthalmoscopy. Ages of the rhesus macaques ranged from 0.7 to 29 years (mean, 16.4 ± 7.5 years). Anterior segment measurements such as intraocular pressure (n = 142), corneal thickness (n = 84), lens thickness (n = 114), and axial length (n = 114) were acquired. Advanced retinal imaging in the form of fundus photography (n = 78), optical coherence tomography (n = 60), and quantitative autofluorescence (n = 44) was obtained. Electroretinography (n = 75) was used to assay retinal function. Quantitative analyses of the macular structure, retinal layer segmentation, and rod and cone photoreceptor electrical responses are reported. Quantitative assessments were made and variations between sexes were analyzed to compare with established sex changes in human eyes.

Conclusions: The rhesus macaque has an ocular structure and function very similar to that of the human eye. In particular macular structure and retinal function is very similar to humans, making this species particularly useful for the study of macular biology and development of therapies for cone photoreceptor disorders.

Translational Relevance: Rhesus macaques are an ideal model for future vision science studies of human eye diseases.
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http://dx.doi.org/10.1167/tvst.10.6.7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107642PMC
May 2021

Hypoxia-inducible exosomes facilitate liver-tropic pre-metastatic niche in colorectal cancer.

Hepatology 2021 Jun 10. Epub 2021 Jun 10.

Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, 210009, China.

Background & Aims: Liver metastasis is a frequent occurrences in patients with colorectal cancer (CRC), 15%-25% of CRC patients have liver metastases at the time of initial diagnosis (1). Specifically, some regional-stage patients with mild symptoms (stage Ⅰ or II) will also advance to liver metastases rapidly, even CRC lesion in situ is resected in time. Nevertheless, the precise mechanism of liver metastasis is still unclear.

Approach & Results: Fresh tumor tissues from CRC patients, adjacent noncancerous tissues (ANT) and colorectal adenoma tissues (ADE) were subjected to microarray analysis to identify differentially expressed miRNA. Exosomes from human serum and cell culture medium were separated, quantitated and verified by transmission electronic microscopy and Zetasizer Nano. Luciferase reporter assay, real-time qPCR, western blot, immunoprecipitation, chromatin and re-chromatin immunoprecipitation, migration and invasion assay, PDX mice model, flow cytometry, immunohistochemistry and immunofluorescence staining were employed to explore the regulation among CRC liver metastases, immunosuppression and cell adhesion. In this study, we demonstrated that the hypoxic microenvironment in primary CRC lesions boosted exosome release, selectively initiated a favorable pre-metastatic niche formation in the hepar, but not in other organs. Mechanistically, Kupffer cells (KCs) can phagocytose exosomes contained highly expressed miR-135a-5p from blood circulation into the liver. The exosomal miR-135a-5p initiated LATS2-YAP-MMP7 axis to promote the occurrences of CRC liver metastasis, and CD30-TRAF2-p65-mediated immunosuppression signaling also contributed to this process.

Conclusions: Hypoxia-induced exosomal miR-135a-5p correlates with the development, clinical severity and prognosis of CRC liver metastases through the pre-metastatic niche, and our findings revealed that miR-135a-5p might be a promising target in halting CRC liver metastases.
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http://dx.doi.org/10.1002/hep.32009DOI Listing
June 2021

Clinical and Prognostic Significance of CD117 in Non-Small Cell Lung Cancer: A Systemic Meta-Analysis.

Pathobiology 2021 Jun 9:1-10. Epub 2021 Jun 9.

Department of Oncology, People's Hospital of Xinjiang Uygur, Urumqi, China.

The aim of this study was to assess the relationship of cluster of differentiation 117 (CD117) expression with the clinicopathological characteristics and the prognosis in patients with non-small cell lung cancer (NSCLC). No meta-analysis concerning the correlation of CD117 expression with clinical and prognostic values of the patients with NSCLC is reported. A systematic literature search was conducted to achieve eligible studies. The combined odds ratios (ORs) or hazard ratios (HRs: multivariate Cox analysis) with their 95% confidence intervals (CIs) were calculated in this analysis. Final 17 eligible studies with 4,893 NSCLC patients using immunohistochemical detection were included in this meta-analysis. CD117 expression was not correlated with gender (male vs. female), clinical stage (stages 3-4 vs. stages 1-2), tumor grade (grade 3 vs. grades 1-2), T-stage (T-stages 3-4 vs. T-stages 0-2), distal metastasis, and disease-free survival (DFS) of NSCLC (all p values >0.05). CD117 expression was associated with lymph node metastasis (positive vs. negative: OR = 0.74, 95% CI = 0.56-0.97, p = 0.03), histological type (adenocarcinoma (AC) versus squamous cell carcinoma (SCC): OR = 1.74, 95% CI = 1.26-2.39, p = 0.001), and a worse overall survival (OS) (HR = 1.89, 95% CI = 1.22-2.92, p = 0.004). The expression of CD117 was significantly higher in AC than in SCC. CD117 may be an independent prognostic indicator for worse OS in NSCLC.
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http://dx.doi.org/10.1159/000514386DOI Listing
June 2021

Transcript isoforms of Reep6 have distinct functions in the retina.

Hum Mol Genet 2021 Jun 8. Epub 2021 Jun 8.

Human Genome Sequencing Center, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, 77030, USA.

Much of the complexity of the eukaryotic cell transcriptome is due to the alternative splicing of mRNA. However, knowledge on how transcriptome complexity is translated into functional complexity remains limited. For example, although different isoforms of a gene may show distinct temporal and spatial expression patterns, it is largely unknown whether these isoforms encode proteins with distinct functions matching their expression pattern. In this report, we investigated the function and relationship of the two isoforms of Reep6, namely Reep6.1 and Reep6.2, in rod photoreceptor cells. These two isoforms result from the alternative splicing of exon 5 and show mutually exclusive expression patterns. Reep6.2 is the canonical isoform that is expressed in non-retinal tissues while Reep6.1 is the only expressed isoform in the adult retina. The Reep6.1 isoform-specific knockout mouse, Reep6E5/E5, is generated by deleting exon 5 and a homozygous deletion phenotypically displayed a rod degeneration phenotype comparable to a Reep6 full knockout mouse, indicating that the Reep6.1 isoform is essential for the rod photoreceptor cell survival. Consistent with the results obtained from a loss-of-function experiment, overexpression of Reep6.2 failed to rescue the rod degeneration phenotype of Reep6 knockout mice while overexpression of Reep6.1 does lead to rescue. These results demonstrate that, consistent with the expression pattern of the isoform, Reep6.1 has rod-specific functions that cannot be substituted by its canonical isoform. Our findings suggested that a strict regulation of splicing is required for the maintenance of photoreceptor cells.
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http://dx.doi.org/10.1093/hmg/ddab157DOI Listing
June 2021

Ecological risk assessment of heavy metals in farmland soils in Beijing by three improved risk assessment methods.

Environ Sci Pollut Res Int 2021 Jun 8. Epub 2021 Jun 8.

Agro-Environmental Protection Institute Ministry of Agriculture and Rural Affairs, Tianjin, 300091, China.

Beijing, as the capital of China, still has soil pollution problems that cannot be ignored. However, there are few studies on the overall ecological risks of heavy metals in farmland soils in Beijing. This study selected 432 soils and crops heavy metal content data of eight districts in Beijing from academic papers and academic journal papers. In this study, the improved Hakanson method, improved analytic hierarchy process (AHP), and integrated quality impact index (IICQ) comprehensively were used to evaluate the impact of Pb, As, and Cd pollution on the farmland soil environment, and the applicable conditions of these methods were discussed. The results by improved Hakanson method showed that both Pb and As were at the normal ecological risk level, while Cd was the largest contributor to potential ecological risk which accounted for 72.54% of the total risk and is mainly at a moderate ecological risk level. The analysis by improved AHP showed that the average comprehensive index of soil heavy metal pollution in the study area was 0.2317, which was at a light pollution level. The IICQ of soil and agricultural products were between 0 and 1 demonstrating that the soil was clean. In summary, the pollution of heavy metals Pb, As, and Cd in the study area is at a relatively low level, and there is no significant risk to the surrounding environment and human health. IICQ method is suitable for the evaluation of soil heavy metal composition and individual impact and can be more accurately used for the overall ecological evaluation of soil-crop-human health system.
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http://dx.doi.org/10.1007/s11356-021-14695-8DOI Listing
June 2021

Epstein-Barr virus positive diffuse large B-cell lymphoma transformed into angioimmunoblastic T-cell lymphoma after treatment.

Clin Case Rep 2021 May 5;9(5):e04083. Epub 2021 May 5.

Department of Pathology Chongqing Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment Chongqing University Cancer Hospital Chongqing China.

Angioimmunoblastic T-cell lymphoma (AITL) is the subtype of mature T-cell non-Hodgkin lymphoma. Compared with diffuse large B-cell lymphoma (DLBCL), AITL patients are frequently accompanied with Epstein-Barr virus (EBV) infection. To date, there is no report on the subsequent development of AITL in patients with EBV-positive DLBCL. We performed a rare case of EBV-positive AITL developing one year after initial diagnosis of EBV-positive DLBCL. The patient showed poor response to the chemotherapy regimen, and poor survival.
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http://dx.doi.org/10.1002/ccr3.4083DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8142414PMC
May 2021

An L314Q mutation in Map3k1 gene results in failure of eyelid fusion in the N-ethyl-N-nitrosourea-induced mutant line.

Exp Anim 2021 Jun 2. Epub 2021 Jun 2.

Institute of Comparative Medicine, Yangzhou University.

In this study, we describe an N-ethyl-N-nitrosourea-induced mouse model with a corneal opacity phenotype that was associated with "eye open at birth" (EOB). Histological and immunohistochemistry staining analysis showed abnormal differentiation of the corneal epithelial cells in the mutant mice. The EOB phenotype was dominantly inherited on a C57BL/6 (B6) background. This allele carries a T941A substitution in exon 4 that leads to an L314Q amino acid change in the open reading frame of MAP3K1 (MEEK1). We named this novel Map3k1 allele Map3k1. Phalloidin staining of F-actin was reduced in the mutant epithelial leading edge cells, which is indicative of abnormality in epithelial cell migration. Interestingly enough, not only p-c-Jun and p-JNK but also c-Jun levels were decreased in the mutant epithelial leading edge cells. This study identifies a novel mouse Map3k1 allele causing EOB phenotype and the EOB phenotype in Map3k1 mousemay be associated with the reduced level of p-JNK and c-Jun.
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http://dx.doi.org/10.1538/expanim.21-0005DOI Listing
June 2021

Induction of the Proinflammatory Chemokine Interleukin-8 Is Regulated by Integrated Stress Response and AP-1 Family Proteins Activated during Coronavirus Infection.

Int J Mol Sci 2021 May 26;22(11). Epub 2021 May 26.

Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou 510642, China.

Infection induces the production of proinflammatory cytokines and chemokines such as interleukin-8 (IL-8) and IL-6. Although they facilitate local antiviral immunity, their excessive release leads to life-threatening cytokine release syndrome, exemplified by the severe cases of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. In this study, we investigated the roles of the integrated stress response (ISR) and activator protein-1 (AP-1) family proteins in regulating coronavirus-induced IL-8 and IL-6 upregulation. The mRNA expression of IL-8 and IL-6 was significantly induced in cells infected with infectious bronchitis virus (IBV), a gammacoronavirus, and porcine epidemic diarrhea virus, an alphacoronavirus. Overexpression of a constitutively active phosphomimetic mutant of eukaryotic translation initiation factor 2α (eIF2α), chemical inhibition of its dephosphorylation, or overexpression of its upstream double-stranded RNA-dependent protein kinase (PKR) significantly enhanced IL-8 mRNA expression in IBV-infected cells. Overexpression of the AP-1 protein cJUN or its upstream kinase also increased the IBV-induced IL-8 mRNA expression, which was synergistically enhanced by overexpression of cFOS. Taken together, this study demonstrated the important regulatory roles of ISR and AP-1 proteins in IL-8 production during coronavirus infection, highlighting the complex interactions between cellular stress pathways and the innate immune response.
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http://dx.doi.org/10.3390/ijms22115646DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198748PMC
May 2021

Research on Enhanced Detection of Benzoic Acid Additives in Liquid Food Based on Terahertz Metamaterial Devices.

Sensors (Basel) 2021 May 7;21(9). Epub 2021 May 7.

School of Mechatronics & Vehicle Engineering, East China Jiaotong University, Nanchang 330013, China.

It is very important for human health to supervise the use of food additives, because excessive use of food additives will cause harm to the human body, especially lead to organ failures and even cancers. Therefore, it is important to realize high-sensibility detection of benzoic acid, a widely used food additive. Based on the theory of electromagnetism, this research attempts to design a terahertz-enhanced metamaterial resonator, using a metamaterial resonator to achieve enhanced detection of benzoic acid additives by using terahertz technology. The absorption peak of the metamaterial resonator is designed to be 1.95 THz, and the effectiveness of the metamaterial resonator is verified. Firstly, the original THz spectra of benzoic acid aqueous solution samples based on metamaterial are collected. Secondly, smoothing, multivariate scattering correction (MSC), and smoothing combined with first derivative (SG + 1 D) methods are used to preprocess the spectra to study the better spectral pretreatment methods. Then, Uninformative Variable Elimination (UVE) and Competitive Adaptive Reweighted Sampling (CARS) are used to explore the optimal terahertz band selection method. Finally, Partial Least Squares (PLS) and Least square support vector machine (LS-SVM) models are established, respectively, to realize the enhanced detection of benzoic acid additives. The LS-SVM model combined with CARS has the best effect, with the correlation coefficient of prediction set (R) is 0.9953, the root mean square error of prediction set (RMSEP) is 7.3 × 10, and the limit of detection (LOD) is 2.3610 × 10 g/mL. The research results lay a foundation for THz spectral analysis of benzoic acid additives, so that THz technology-based detection of benzoic acid additives in food can reach requirements stipulated in the national standard. This research is of great significance for promoting the detection and analysis of trace additives in food, whose results can also serve as a reference to the detection of antibiotic residues, banned additives, and other trace substances.
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http://dx.doi.org/10.3390/s21093238DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8125531PMC
May 2021

Role of Two Plant Growth-Promoting Bacteria in Remediating Cadmium-Contaminated Soil Combined with (Lab.).

Plants (Basel) 2021 May 2;10(5). Epub 2021 May 2.

College of Bioscience and Biotechnology, College of Resource and Environment, Hunan Agricultural University, Changsha 410128, China.

spp. are energy plants and excellent candidates for phytoremediation approaches of metal(loid)s-contaminated soils, especially when combined with plant growth-promoting bacteria. Forty-one bacterial strains were isolated from the rhizosphere soils and roots tissue of five dominant plants ( Levl., Vaniot Houtt, L., Tenore, and Lab.) colonizing a cadmium (Cd)-contaminated mining area (Huayuan, Hunan, China). We subsequently tested their plant growth-promoting (PGP) traits (e.g., production of indole-3-acetic acid, siderophore, and 1-aminocyclopropane-1-carboxylate deaminase) and Cd tolerance. Among bacteria, two strains, TS8 and MR2, presented higher Cd tolerance and showed the best results regarding in vitro growth-promoting traits. In the subsequent pot experiments using soil spiked with 10 mg Cd·kg, we investigated the effects of TS8 and MR2 strains on soil Cd phytoremediation when combined with (Lab.). After sixty days of planting (Lab.), we found that TS8 increased plant height by 39.9%, dry weight of leaves by 99.1%, and the total Cd in the rhizosphere soil was reduced by 49.2%. Although MR2 had no significant effects on the efficiency of phytoremediation, it significantly enhanced the Cd translocation from the root to the aboveground tissues (translocation factor > 1). The combination of TS8 and (Lab.) may be an effective method to remediate Cd-contaminated soils, while the inoculation of MR2 may be used to enhance the phytoextraction potential of .
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http://dx.doi.org/10.3390/plants10050912DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147505PMC
May 2021

[Spatial and temporal variations of vegetation coverage in the middle section of Yellow River Basin based on terrain gradient:Taking Yan'an City as an example.]

Ying Yong Sheng Tai Xue Bao 2021 May;32(5):1581-1592

School of Earth Sciences and Resources, Chang'an University, Xi'an 710054, China.

Vegetation restoration is an important requirement for ecological protection and high-quality development in the Yellow River Basin. In-depth understanding the temporal and spatial differentiation of vegetation has important practical significance. With the four Landsat TM/OLI remote sensing images as the data source, the pixel binary model was used to estimate vegetation coverage. The transfer matrix, geological information map, and the center of gravity migration model were used to analyze the temporal and spatial variations of vegetation coverage in Yan'an City in the middle of the Yellow River Basin from 1988 to 2018. The spatial variation of vegetation cover to elevation and slope was explored using terrain distribution index combined with terrain data. The results showed that vegetation coverage in Yan'an was low in the north region and high in the south region. The vegetation coverage increased significantly due to policy changes. From 1988 to 2018, the vegetation change pattern in Yan'an was dominated by continuous improvement and stability. Vegetation coverage increased in about 50% of the area. 83% of the study area with high vegetation coverage remained stable. At each elevation and slope level, the advantage of high vegetation cove-rage increased with time. At each slope level, vegetation cover increased with slope. The transition direction of vegetation cover of different levels in Yan'an was basically consistent with the overall spatial change trend of vegetation cover, with the overall shift being from north to west. The vegetation construction in Yan'an has achieved remarkable results, though the vegetation coverage in the north still needs to be improved. Optimizing vegetation types and structures is an important direction for future vegetation construction.
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http://dx.doi.org/10.13287/j.1001-9332.202105.014DOI Listing
May 2021

Thermally Assisted Rashba Splitting and Circular Photogalvanic Effect in Aqueously Synthesized 2D Dion-Jacobson Perovskite Crystals.

Nano Lett 2021 Jun 26;21(11):4584-4591. Epub 2021 May 26.

School of Optical and Electronic Information, Huazhong University of Science and Technology, Wuhan, 430074, China.

Recently, a two-dimensional Dion-Jacobson (DJ) perovskite (AMP)PbI (AMP = 4-(aminomethyl)piperidinium) is emerging with remarkable Rashba effect and ferroelectricity. However, the origin of the giant Rashba splitting remains elusive and the current synthetic strategy via slow cooling is time- and power-consuming, hindering its future applications. Here, we report on an economical aqueous method to obtain (AMP)PbI crystals and clarify the origin of the giant Rashba effect by temperature- and polarization-dependent photoluminescence (PL) spectroscopy. The strong temperature-dependent PL helicity indicates the thermally assisted structural distortion as the main origin of the Rashba effect, suggesting that valley polarization still preserves at high temperatures. The Rashba effect was further confirmed by the circular photogalvanic effect near the indirect bandgap. Our study not only optimizes the synthetic strategies of this DJ perovskite but also sheds light on its potential applications in room/high-temperature spintronics and valleytronics.
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http://dx.doi.org/10.1021/acs.nanolett.1c00364DOI Listing
June 2021

Corilagin prevents SARS-CoV-2 infection by targeting RBD-ACE2 binding.

Phytomedicine 2021 Jul 5;87:153591. Epub 2021 May 5.

Dr. Neher's Biophysics Laboratory for Innovative Drug Discovery, State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macau, China. Electronic address:

Background: The outbreak of coronavirus (SARS-CoV-2) disease caused more than 100,000,000 people get infected and over 2,200,000 people being killed worldwide. However, the current developed vaccines or drugs may be not effective in preventing the pandemic of COVID-19 due to the mutations of coronavirus and the severe side effects of the newly developed vaccines. Chinese herbal medicines and their active components play important antiviral activities. Corilagin exhibited antiviral effect on human immunodeficiency virus (HIV), hepatitis C virus (HCV) and Epstein-Barr virus (EBV). However, whether it blocks the interaction between SARS-CoV-2 RBD and hACE2 has not been elucidated.

Purpose: To characterize an active compound, corilagin derived from Phyllanthus urinaria as potential SARS-CoV-2 entry inhibitors for its possible preventive application in daily anti-virus hygienic products.

Methods: Computational docking coupled with bio-layer interferometry, BLI were adopted to screen more than 1800 natural compounds for the identification of SARS-CoV-2 spike-RBD inhibitors. Corilagin was confirmed to have a strong binding affinity with SARS-CoV-2-RBD or human ACE2 (hACE2) protein by the BLI, ELISA and immunocytochemistry (ICC) assay. Furthermore, the inhibitory effect of viral infection of corilagin was assessed by in vitro pseudovirus system. Finally, the toxicity of corilagin was examined by using MTT assay and maximal tolerated dose (MTD) studies in C57BL/6 mice.

Results: Corilagin preferentially binds to a pocket that contains residues Cys 336 to Phe 374 of spike-RBD with a relatively low binding energy of -9.4 kcal/mol. BLI assay further confirmed that corilagin exhibits a relatively strong binding affinity to SARS-CoV-2-RBD and hACE2 protein. In addition, corilagin dose-dependently blocks SARS-CoV-2-RBD binding and abolishes the infectious property of RBD-pseudotyped lentivirus in hACE2 overexpressing HEK293 cells, which mimicked the entry of SARS-CoV-2 virus in human host cells. Finally, in vivo studies revealed that up to 300 mg/kg/day of corilagin was safe in C57BL/6 mice. Our findings suggest that corilagin could be a safe and potential antiviral agent against the COVID-19 acting through the blockade of the fusion of SARS-CoV-2 spike-RBD to hACE2 receptors.

Conclusion: Corilagin could be considered as a safe and environmental friendly anti-SARS-CoV-2 agent for its potential preventive application in daily anti-virus hygienic products.
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http://dx.doi.org/10.1016/j.phymed.2021.153591DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8098048PMC
July 2021

Evaluation of the photocatalytic performance of molecularly imprinted S-TiO by paper microzones.

Environ Res 2021 Aug 23;199:111258. Epub 2021 May 23.

School of Urban Construction, Wuhan University of Science and Technology, Wuhan, 430065, China. Electronic address:

The paper microzones method (PMZs) is a green chemical method that uses the principle of the three primary colors of red, green and blue (RGB) to detect the water quality of the droplets on white paper. However, this method is rarely used in the performance evaluation of photocatalysts. The paper details the first use of paper microzones utilized in the evaluation of photocatalyst performance. A sol-gel method was used to prepare molecularly imprinted modified TiO photocatalysts for the treatment of different wastewaters, and characterized the catalysts using XRD and several other methods. The reliability of PMZs on the evaluation of photocatalytic activity and selectivity was also analyzed. The following results were obtained: EP-TiO catalysts (EP, ethyl paraben, the imprinting molecule) with different S doping levels were synthesized using a one-step sol-gel method, and the best S doping ratio was found to be n(Ti):n(S) 3:1. S-EP-TiO was found to be 100% anatase and showed excellent photocatalytic performance, while the PMZs method accurately determined changes in RGB levels for the photocatalytic degradation process of pollutants using S-EP-TiO as the photocatalyst. A photocatalytic kinetic analysis showed the PMZs method was quite suitable for the evaluation of photocatalyst activity, but the evaluation of selectivity needs improvement. This method is a promising green chemistry way to evaluate photocatalyst performance and the rapid detection of outdoor sewage water quality.
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http://dx.doi.org/10.1016/j.envres.2021.111258DOI Listing
August 2021

Contributions of Costimulatory Molecule CD137 in Endothelial Cells.

J Am Heart Assoc 2021 Jun 22;10(11):e020721. Epub 2021 May 22.

Department of Geriatrics Union Hospital Tongji Medical CollegeHuazhong University of Science and Technology Wuhan China.

CD137 (4-1BB, tumor necrosis factor receptor superfamily 9) is a surface glycoprotein of the tumor necrosis factor receptor family that can be induced on a variety of immunocytes and nonimmune cells, including endothelial cells and smooth muscle cells. The importance of CD137 in immune response has been well recognized; however, the precise biological effects and underlying mechanisms of CD137 in endothelial cells are unclear. A single layer of cells called the endothelium constitutes the innermost layer of blood vessels including larger arteries, veins, the capillaries, and the lymphatic vessels. It not only acts as an important functional interface, but also participates in local inflammatory response. This review covers recent findings to illuminate the role of CD137 in endothelial cells in different pathophysiologic settings.
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http://dx.doi.org/10.1161/JAHA.120.020721DOI Listing
June 2021

Efficacy and tolerability of lamotrigine in the treatment of focal epilepsy among children and adolescents: a meta-analysis.

Transl Pediatr 2021 Apr;10(4):807-818

Center for Evidence-Based Medicine, Capital Institute of Pediatrics, Beijing, China.

Background: Epilepsy is the most common chronic neurological disease in children, and focal epileptic seizures are the most common subtype. Unlike the data supporting treatment options for adults with epilepsy, evidence regarding the most effective first-line drug therapy for focal epilepsy in children and adolescents is limited. While lamotrigine is a therapeutic option for adults, there are disagreements surrounding its efficacy and tolerability in the younger population. Therefore, we performed a meta-analysis to determine if there was sufficient evidence to support a more definitive recommendation.

Methods: We undertook electronic search strategies using Medline via Ovid SP, Embase via Ovid SP up to February 05, 2021. We also searched relevant articles through Chinese BioMedical Literature (CBM), Chinese National Knowledge Infrastructure (CNKI), WANFANG, and VIP databases up to February 05, 2021. Study selection and data extraction were performed by 2 authors independently. The randomized controlled trials on focal epilepsy in children were included, and we made risk of bias judgments based on the methods endorsed by The Cochrane Collaboration. We used fifty percent or greater reduction in seizure frequency as an indicator of efficacy, the incidence of adverse events and treatment withdrawal as indicators of tolerability. The strength of the correlation was assessed via risk ratios (RRs) and their 95% confidence intervals (95% CIs).

Results: A total of 7 randomized trials involving 757 participants fulfilled the eligibility criteria. Of the 7 trials, 3 were placebo-controlled, and 4 compared lamotrigine with carbamazepine or oxcarbazepine. Lamotrigine was significantly more effective than placebo in achieving ≥50% reduction in seizure frequency, but its efficacy was not significantly different from that of carbamazepine or oxcarbazepine (lamotrigine placebo: RR 2.95, 95% CI, 1.88 to 4.61; lamotrigine carbamazepine/oxcarbazepine: RR 0.95, 95% CI, 0.85 to 1.05. There was significant difference in the incidence of overall adverse events between the lamotrigine- and carbamazepine/oxcarbazepine-treated groups (RR 0.64, 95% CI, 0.45 to 0.90).

Conclusions: Lamotrigine was effective in reducing the seizure frequency when used as an add-on treatment in children with focal epilepsy, but current evidence does not suggest that lamotrigine is superior to carbamazepine/oxcarbazepine as monotherapy. For overall adverse events, lamotrigine has significantly fewer than carbamazepine/oxcarbazepine, suggesting that lamotrigine has better tolerability.
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http://dx.doi.org/10.21037/tp-20-379DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107846PMC
April 2021

Genomes of 12 fig wasps provide insights into the adaptation of pollinators to fig syconia.

J Genet Genomics 2021 Mar 29. Epub 2021 Mar 29.

Institute of Entomology, College of Life Sciences, Nankai University, Tianjin 300071, China; Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. Electronic address:

Figs and fig pollinators are one of the few classic textbook examples of obligate pollination mutualism. The specific dependence of fig pollinators on the relatively safe living environment with sufficient food sources in the enclosed fig syconia implies that they are vulnerable to habitat changes. However, there is still no extensive genomic evidence to reveal the evolutionary footprint of this long-term mutually beneficial symbiosis in fig pollinators. In fig syconia, there are also non-pollinator species. The non-pollinator species differ in their evolutionary and life histories from pollinators. We conducted comparative analyses on 11 newly sequenced fig wasp genomes and one previously published genome. The pollinators colonized the figs approximately 66.9 million years ago, consistent with the origin of host figs. Compared with non-pollinators, many more genes in pollinators were subject to relaxed selection. Seven genes were absent in pollinators in response to environmental stress and immune activation. Pollinators had more streamlined gene repertoires in the innate immune system, chemosensory toolbox, and detoxification system. Our results provide genomic evidence for the differentiation between pollinators and nonpollinators. The data suggest that owing to the long-term adaptation to the fig, some genes related to functions no longer required are absent in pollinators.
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http://dx.doi.org/10.1016/j.jgg.2021.02.010DOI Listing
March 2021

Platelet-to-lymphocyte ratio and C-reactive protein as markers for colorectal polyp histological type.

BMC Cancer 2021 May 17;21(1):556. Epub 2021 May 17.

Department of Gastroenterology, Shandong Provincial Hospital Affiliated to Shandong First Medical University & Shandong Academy of Medical Sciences, 324 Jingwu Road, Jinan, 250021, Shandong, People's Republic of China.

Background: The platelet-to-lymphocyte ratio (PLR) and C-reactive protein (CRP) level are markers that have been reported to predict the histological type of various tumors, and here, we evaluated their utility in predicting colorectal polyp histological types.

Methods: We retrospectively reviewed 172 patients with colorectal polyps who underwent endoscopic polypectomy. The associations between histological type and clinicopathologic parameters were assessed by multivariate analysis.

Results: The optimal PLR and CRP cut-off values were 113.32 and 0.39, respectively. The PLR (P = 0.002) and CRP (P = 0.009) values were associated with the histological type according to the univariate analysis, whereas low PLR (P ≤ 0.001) and CRP (P = 0.017) values were independent risk factors in the multivariate analysis together with maximum tumor diameter (P ≤ 0.001) and tumor number (P = 0.0014).

Conclusions: Preoperative PLR and CRP are correlated with the colorectal polyp histological type.
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http://dx.doi.org/10.1186/s12885-021-08221-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8127289PMC
May 2021

Blink-sensing glasses: A flexible iontronic sensing wearable for continuous blink monitoring.

iScience 2021 May 3;24(5):102399. Epub 2021 Apr 3.

Institute of Biomedical and Health Engineering, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.

Blink reflex has long been considered closely related to physiological states, from which abundant information on ocular health and activities can be revealed. In this study, a smart glasses wearable has been developed, incorporating a flexible and sensitive pressure sensor, to monitor blink patterns by continuously detecting ocular muscular movements, referred to as blink-sensing glasses. By applying the emerging flexible iontronic sensing (FITS) sensor with the sensitivity of 340 pF/mmHg, the skin pressure variations induced by movements of the orbicularis oculi muscles can be monitored in real time. The blink-sensing glasses can successfully capture blink patterns with a high accuracy of 96.3% and have been used to differentiate the blink features from both dry-eye subjects and healthy controls. This device can be potentially used as a new clinical and research monitoring tool for continuous eye blink analysis, while providing patients with high comfortableness in long-term ambulatory and home settings.
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http://dx.doi.org/10.1016/j.isci.2021.102399DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8102906PMC
May 2021

Association of cardiovascular disease with 30-day hospital readmission in Chinese patients receiving maintenance dialysis.

Ann Transl Med 2021 Apr;9(8):617

Department of Medicine, Peking University First Hospital, Beijing, China.

Background: Previous studies have shown cardiovascular disease (CVD) to be a risk factor in the prediction of 30-day hospital readmission among patients receiving dialysis. However, studies of Asian populations are limited. In the present study, we examined the association between CVD and 30-day hospital readmission in Chinese patients receiving maintenance dialysis.

Methods: Patients receiving maintenance dialysis were identified by searching a national claims database, the China Health Insurance Research Association (CHIRA) database, using the International Classification of Diseases revision 10 (ICD-10) and items of medical service claims. Patients aged ≥18 years who were discharged after index hospitalization between January 2015 and December 2015 were included in our retrospective analysis. CVD-related diagnoses were divided into three categories: coronary heart disease (CHD), heart failure (HF), and stroke. Thirty-day hospital readmission was defined as any hospital readmission within the 30 days following discharge. Logistic regression models adjusted for logit of propensity scores (PS) were used to assess the association of CVD with 30-day hospital readmission.

Results: Of 4,700 patients receiving dialysis, the 30-day hospital readmission rate was 10.4%. Compared with patients without CVD, there was an increased risk of 30-day hospital readmission among maintenance dialysis patients with total CVD [odds ratio (OR): 1.33, 95% confidence interval (CI): 1.06-1.66]. Patients with HF (OR: 1.77, CI: 1.27-2.47) and stroke (OR: 2.14, 95% CI: 1.53-2.98) had a greater risk of 30-day hospital readmission. The fully adjusted OR of CHD for the risk of 30-day hospital readmission was 1.22 (95% CI: 0.97-1.55).

Conclusions: CVDs, especially stroke and HF, are independent predictors of 30-day hospital readmission in Chinese patients receiving dialysis, and could help to guide interventions to improve the quality of care for these patients.
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http://dx.doi.org/10.21037/atm-20-2367DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106029PMC
April 2021

[Trend of atmosphreic PM_(2. 5) and their components in two districts of Lanzhou City from 2015 to 2019].

Wei Sheng Yan Jiu 2021 Mar;50(2):274-283

Gansu Provincial Center for Disease Control and Prevention, Lanzhou 730000, China.

Objective: To analyse the variation of the fine particulate matter(PM_(2. 5)) concentration and its chemical composition in two districts of Lanzhou City from 2015 to 2019.

Methods: From January 2015 to December 2019, two sampling points, namely residential district(Chengguan District) and industrial district(Xigu District) were selected in Lanzhou City, to collect PM_(2. 5) samples, and the mass concentrations, metals and metalloid elements, water-soluble ions and polycyclic aromatic hydrocarbons(PAHs) contents were determined.

Results: A total of 838 samples were collected in Chengguan District and Xigu District in Lanzhou City during 2015-2019, the average annual mass concentration and the daily average concentration of the PM_(2. 5) were decreasing year by year(P<0. 05). The higher metal content in PM_(2. 5) were Al, Pb, Mn and As during 2015-2019. Sb, Al, As, Cd, Cr, Pb, Mn and Tl element content were higher in 2015 and 2016 than in other years(P<0. 05), the average annual concentration of As were 7. 92 and 8. 63 ng/m~3 respectively, which exceeded the reference concentration limit of the ambient air quality standard. The average annual mass concentration of Cr from 2015 to 2019 was much higher than the annual average reference concentration limit of the ambient air quality standard. From 2015 to 2019, except for 2018, the highest average concentration of PAHs in atmospheric PM_(2. 5) in the two districts of Lanzhou City was Ant, B(b)F and I(cd)P mass concentration were highest in 2015, N(a)P, FI, Ace, Phe, Ant, Flu, Pyr, B(k)F and B(a)P mass concentration were highest in 2017, Acy, Chr and B(ghi)P mass concentration were highest in 2018(P<0. 05). From 2017 to 2018, the average annual content of B(a)P in the two districts of Lanzhou City were 1. 16 and 1. 11 ng/m~3, respectively, which exceeded the annual average concentration limit of the ambient air quality standard. The concentration of Cl~- in PM_(2. 5) in the two districts of Lanzhou City was decreasing year by year, the annual average concentrations of SO~2-_4, NO~-_3 and NH~+_4 from 2017 to 2019 showed a downward trend year by year. SO~2-_4, Cl~- of both districts were the highest in 2015, and NO~-_3, NH~+_4 were the highest in 2017(P<0. 05).

Conclusion: The air quality of Lanzhou City has been improved obviously, but the pollution problem is still serious.
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http://dx.doi.org/10.19813/j.cnki.weishengyanjiu.2021.02.018DOI Listing
March 2021

Focus shaping of high numerical aperture lens using physics-assisted artificial neural networks.

Opt Express 2021 Apr;29(9):13011-13024

We present a physics-assisted artificial neural network (PhyANN) scheme to efficiently achieve focus shaping of high numerical aperture lens using a diffractive optical element (DOE) divided into a series of annular regions with fixed widths. Unlike the conventional ANN, the PhyANN does not require the training using labeled data, and instead output the transmission coefficients of each annular region of the DOE by fitting weights of networks to minimize the delicately designed loss function in term of focus profiles. Several focus shapes including sub-diffraction spot, flattop spot, optical needle, and multi-focus region are successfully obtained. For instance, we achieve an optical needle with 10λ depth of focus, 0.41λ lateral resolution beyond diffraction limit and high flatness of almost the same intensity distribution. Compared to typical particle swarm optimization algorithm, the PhyANN has an advantage in DOE design that generates three-dimensional focus profile. Further, the hyperparameters of the proposed PhyANN scheme are also discussed. It is expected that the obtained results benefit various applications including super-resolution imaging, optical trapping, optical lithography and so on.
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http://dx.doi.org/10.1364/OE.421354DOI Listing
April 2021

All-Inorganic Quantum-Dot LEDs Based on a Phase-Stabilized α-CsPbI3 Perovskite.

Angew Chem Int Ed Engl 2021 May 12. Epub 2021 May 12.

Department of Electrical and Computer Engineering, University of Toronto, 10 King's College Road, Toronto, Ontario, M5S 3G4, Canada.

The all-inorganic nature of CsPbI perovskites allows to enhance stability in perovskite devices. Research efforts have led to improved stability of the black phase in CsPbI films; however, these strategies-including strain and doping-are based on organic-ligand-capped perovskites, which prevent perovskites from forming the close-packed quantum dot (QD) solids necessary to achieve high charge and thermal transport. We developed an inorganic ligand exchange that leads to CsPbI QD films with superior phase stability and increased thermal transport. The atomic-ligand-exchanged QD films, once mechanically coupled, exhibit improved phase stability, and we link this to distributing strain across the film. Operando measurements of the temperature of the LEDs indicate that KI-exchanged QD films exhibit increased thermal transport compared to controls that rely on organic ligands. The LEDs exhibit a maximum EQE of 23 % with an electroluminescence emission centered at 640 nm (FWHM: ≈31 nm). These red LEDs provide an operating half-lifetime of 10 h (luminance of 200 cd m ) and an operating stability that is 6× higher than that of control devices.
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http://dx.doi.org/10.1002/anie.202104812DOI Listing
May 2021

Plasma fibrinogen acts as a predictive factor for pathological complete response to neoadjuvant chemotherapy in breast cancer: a retrospective study of 1004 Chinese breast cancer patients.

BMC Cancer 2021 May 12;21(1):542. Epub 2021 May 12.

Department of Endocrine and Breast Surgery, The First Affiliated Hospital of Chongqing Medical University, No.1 Youyi Road, Yuzhong District, Chongqing, 400016, China.

Background: The aim of this study was to evaluate the relationship between pre-treatment plasma fibrinogen (Fib) level and pathological complete response (pCR) to neoadjuvant chemotherapy (NAC) in breast cancer patients and to assess the role of plasma Fib as a predictive factor.

Methods: Data from 1004 consecutive patients with invasive breast cancer who received NAC and subsequent surgery were retrospectively analysed. Both univariate and multivariate analyses based on logistic regression model were performed to identify clinicopathological factors associated with pCR to NAC. Cox regression model was used to determine the correlation between clinical or pathological parameters and recurrence-free survival (RFS). The Kaplan-Meier method and the log-rank test were applied in the survival analysis.

Results: The median value of Fib, rather than other plasma coagulation parameters, was significantly increased in non-pCR patients compared with pCR patients (P = 0.002). Based on the cut-off value estimated by the receiver operating characteristic (ROC) curve analysis, patients were divided into low or high Fib groups (Fib < 3.435 g/L or ≥ 3.435 g/L). Low Fib levels were significantly associated with premenopausal or perimenopausal status (P <  0.001), tumour size ≤5 cm (P = 0.002), and positive hormone receptor status (P = 0.002). After adjusted for other clinicopathological factors in the multivariate logistic regression model, low Fib status was strongly associated with pCR to NAC (OR = 3.038, 95% CI 1.667-5.537, P <  0.001). Survival analysis showed that patients with low Fib levels exhibited better 3-year RFS compared with patients with high Fib levels in the tumour size>5 cm group (77.5% vs 58.4%, log-rank, P = 0.0168).

Conclusions: This study demonstrates that low pre-treatment plasma Fib (Fib < 3.435 g/L) is an independent predictive factor for pCR to NAC in breast cancer patients. Moreover, T3-featured breast cancer patients with lower Fib level exhibit better RFS outcomes after NAC compared with high Fib status.
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http://dx.doi.org/10.1186/s12885-021-08284-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8114717PMC
May 2021