Publications by authors named "Ruhua Deng"

2 Publications

  • Page 1 of 1

Thioredoxin-1 Rescues MPP/MPTP-Induced Ferroptosis by Increasing Glutathione Peroxidase 4.

Mol Neurobiol 2021 Feb 26. Epub 2021 Feb 26.

Laboratory of Molecular Neurobiology, Medical School, Kunming University of Science and Technology, No.727 Jingming South Road, Kunming, 650500, China.

Parkinson's disease (PD), a common neurodegenerative disease, is typically associated with the loss of dopaminergic neuron in the substantia nigra pars compacta (SNpc). Ferroptosis is a newly identified cell death, which associated with iron accumulation, glutathione (GSH) depletion, lipid peroxidation formation, reactive oxygen species (ROS) accumulation, and glutathione peroxidase 4 (GPX4) reduction. It has been reported that ferroptosis is linked with PD.Thioredoxin-1 (Trx-1) is a redox regulating protein and plays various roles in regulating the activity of transcription factors and inhibiting apoptosis. However, whether Trx-1 plays the role in regulating ferroptosis involved in PD is still unknown. Our present study showed that 1-methyl-4-phenylpyridinium (MPP) decreased cell viability, GPX4, and Trx-1, which were reversed by Ferrostatin-1 (Fer-1) in PC 12 cells and SH-SY5Y cells. Moreover, the decreased GPX4 and GSH, and increased ROS were inhibited by Fer-1 and Trx-1 overexpression. We further repeated that behavior deficits resulted from 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) were improved in Trx-1 overexpression transgenic mice. Trx-1 reversed the decreases of GPX4 and tyrosine hydroxylase (TH) induced by MPTP in the substantia nigra pars compacta (SNpc). Our results suggest that Trx-1 inhibits ferroptosis in PD through regulating GPX4 and GSH.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12035-021-02320-1DOI Listing
February 2021

Detoxification mechanisms, defense responses, and toxicity threshold in the earthworm Eisenia foetida exposed to ciprofloxacin-polluted soils.

Sci Total Environ 2018 Jan 1;612:442-449. Epub 2017 Sep 1.

School of Biological Engineering, Huainan Normal University, Huainan 232038, China.

The widespread application of antibiotics poses health risks for agro-ecosystems. This study examined the effects of ciproflaxin (CIP)-polluted soils (0-51.2mgCIP/kg) on the earthworm Eisenia foetida. The enhanced activities and isozyme levels of superoxide dismutase (SOD) and ascorbate peroxidase after 15days of CIP exposure suggested reactive oxygen species overproduction and thus the generation of oxidatively damaged proteins (e.g., carbonylated proteins) in the earthworms. Under mild CIP stress, the 20S proteasome was capable of degrading most of the damaged proteins independent of ubiquitin. Under severe stress, proteases and endoproteases were up-regulated and maintained the proteolysis as 20S proteasome activity diminished. These observations suggested that, together with glutathione S-transferases, which also participated in the detoxification, 20S proteasome, proteases, endoproteases, and antioxidant enzymes constituted a detoxification and defense system in the earthworms. The biphasic dose responses of these cellular components confirmed that the dose range tested was reasonable for the bioassay of CIP-polluted soils. Our results also demonstrated the potential utility of SOD and ubiquitin as highly sensitive biomarkers in the early bioassay of CIP-polluted soils. Bases on the results, a toxicity threshold for CIP-polluted soils of 3.2-6.4mgCIP/kg soil can be proposed.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2017.08.120DOI Listing
January 2018