Publications by authors named "Rubí Chablé-Villacis"

2 Publications

  • Page 1 of 1

Consolidated Bioprocess for Bioethanol Production from Raw Flour of Seeds Using the Native Strain of Bm-2.

Microorganisms 2019 Oct 23;7(11). Epub 2019 Oct 23.

Renewable Energy Department, Yucatan Center for Scientific Research, Merida 97302, Mexico.

Consolidated bioprocessing (CBP), which integrates biological pretreatment, enzyme production, saccharification, and fermentation, is a promising operational strategy for cost-effective ethanol production from biomass. In this study, the use of a native strain of (Bm-2) was evaluated for bioethanol production from in a CBP. The raw seed flour obtained from the ramon tree contained 61% of starch, indicating its potential as a raw material for bioethanol production. Quantitative assays revealed that the Bm-2 strain produced the amylase enzyme with activity of 193.85 U/mL. The Bm-2 strain showed high tolerance to ethanol stress and was capable of directly producing ethanol from raw flour at a concentration of 13 g/L, with a production yield of 123.4 mL/kg flour. This study demonstrates the potential of Bm-2 for starch-based ethanol production in a consolidated bioprocess to be implemented in the biofuel industry. The residual biomass after fermentation showed an average protein content of 22.5%, suggesting that it could also be considered as a valuable biorefinery co-product for animal feeding.
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http://dx.doi.org/10.3390/microorganisms7110483DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6920830PMC
October 2019

Laccase Gene Expression and Vinasse Biodegradation by Trametes hirsuta Strain Bm-2.

Molecules 2015 Aug 19;20(8):15147-57. Epub 2015 Aug 19.

Departamento de Ingeniería Química y Bioquímica, Instituto Tecnológico de Mérida, Av. Tecnológico Km 5, Mérida 97118, Yucatán, Mexico.

Vinasse is the dark-colored wastewater that is generated by bioethanol distilleries from feedstock molasses. The vinasse that is generated from molasses contains high amounts of pollutants, including phenolic compounds and melanoindin. The goal of this work was to study the expression of laccase genes in the Trametes hirsuta strain Bm-2, isolated in Yucatan, Mexico, in the presence of phenolic compounds, as well as its effectiveness in removing colorants from vinasse. In the presence of all phenolic compounds tested (guaiacol, ferulic acid, and vanillic acid), increased levels of laccase-encoding mRNA were observed. Transcript levels in the presence of guaiacol were 40 times higher than those in the control. The lcc1 and lcc2 genes of T. hirsuta were differentially expressed; guaiacol and vanillin induced the expression of both genes, whereas ferulic acid only induced the expression of lcc2. The discoloration of vinasse was concomitant with the increase in laccase activity. The highest value of enzyme activity (2543.7 U/mL) was obtained in 10% (v/v) vinasse, which corresponded to a 69.2% increase in discoloration. This study demonstrates the potential of the Bm-2 strain of T. hirsuta for the biodegradation of vinasse.
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http://dx.doi.org/10.3390/molecules200815147DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6332155PMC
August 2015