Publications by authors named "Ru Liu"

164 Publications

Direct Bilirubin Levels Predict Long-Term Outcomes in Patients With Acute Coronary Syndrome Under Different Glucose Metabolism Status: A 6.5-Year Cohort Study of Three-Vessel Disease.

Front Cardiovasc Med 2021 12;8:715539. Epub 2021 Aug 12.

Department of Cardiology, Fuwai Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China.

There is controversy over the relationship between bilirubin and coronary artery disease. This study aimed to evaluate the predictive value of direct bilirubin (DB) in patients with complex acute coronary syndrome (ACS). From April 2004 to February 2011, 5,322 ACS patients presenting with three-vessel disease were consecutively enrolled. Disease severity and complexity were determined by SYNTAX score (SS) and SS II. The primary endpoint was all-cause death, and the secondary endpoints were major adverse cardiovascular and cerebrovascular events (MACCE). Stratification of normal glucose regulation, prediabetes, and diabetes was based on a previous diagnosis, hypoglycemic medications, fasting blood glucose, and hemoglobin A1c. Subjects were divided into quartiles according to baseline DB (μmol/L): Q1 (0-1.6), Q2 (1.61-2.20), Q3 (2.21-2.80), and Q4 (>2.80). Multivariable logistic regression analysis showed that DB was an independent predictor of intermediate-high SS. During a median follow-up time of 6.5 years, elevated DB was associated with more all-cause death ( < 0.001) but not MACCE. DB remained to be predictive of all-cause death in the multivariable Cox regression model (Q2 vs. Q1: HR 1.043, 95% CI 0.829-1.312, = 0.719; Q3 vs. Q1: HR 1.248, 95% CI 1.001-1.155, = 0.048; Q4 vs. Q1: HR 1.312, 95% CI 1.063-1.620, = 0.011). When subjects are stratified according to glucose metabolism regulation and treatment strategies, the predictivity of DB was only profound in patients with diabetes or with conservative treatment. Additionally, incorporating DB further improved the discrimination and reclassification abilities of SS II for risk prediction. DB is a potential biomarker for predicting lesion severity and long-term outcomes in ACS patients.
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http://dx.doi.org/10.3389/fcvm.2021.715539DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8387870PMC
August 2021

Usefulness of FT3 to FT4 Ratio to Predict Mortality in Euthyroid Patients With Prior Cardiovascular Events Undergoing PCI: Five-Year Findings From a Large Single-Center Cohort Study.

Front Endocrinol (Lausanne) 2021 5;12:700349. Epub 2021 Jul 5.

Fu Wai Hospital, National Center for Cardiovascular Diseases, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing, China.

Background: In euthyroid patients undergoing percutaneous coronary intervention (PCI), it is still unclear whether free triiodothyronine to free thyroxine (FT3/FT4) ratio can predict the recurrence of cardiovascular events (CVEs). We aim to investigate its association with recurrent long-term adverse events in this population.

Methods: 3549 euthyroid patients with prior CVEs history undergoing PCI were consecutively enrolled in our study and subsequently divided into three FT3/FT4 ratio tertiles (T1<2.41, n=1170; 2.41≤T2<2.75, n=1198; T3>2.75, n=1181). The primary endpoint was major adverse cardiovascular and cerebrovascular event (MACCE), a composite of all-cause death, myocardial infarction, stroke and revascularization. The secondary endpoints were all-cause death and cardiac death.

Results: The median follow-up time was 5 years. The incidence of all-cause death, cardiac death and MACCE were significantly higher among patients in the lowest FT3/FT4 tertile (P<0.05). After adjustment of confounding factors, decreased FT3/FT4 ratio was independently associated with an increased risk of all-cause death (HR 1.82, 95% CI 1.13-2.93, P=0.014), cardiac death (HR 1.90, 95% CI 1.04-3.46, P=0.036) and MACCE (HR 1.33, 95% CI 1.10-1.60, P=0.003) which was driven mainly by all-cause death.

Conclusions: In euthyroid patients with prior cardiovascular events undergoing PCI, FT3/FT4 ratio might be a potential predictor of all-cause and cardiac mortality. Routine assessment of FT3/FT4 ratio might be a simple and effective tool for risk stratification in this specific patient population.
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http://dx.doi.org/10.3389/fendo.2021.700349DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8287966PMC
July 2021

Prognostic value of fibrinogen in patients with coronary artery disease and prediabetes or diabetes following percutaneous coronary intervention: 5-year findings from a large cohort study.

Cardiovasc Diabetol 2021 07 16;20(1):143. Epub 2021 Jul 16.

State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, No 167, Beilishi Road, Xicheng District, Beijing, 100037, China.

Background: Fibrinogen (FIB) is an independent risk factor for mortality and cardiovascular events in the general population. However, the relationship between FIB and long-term mortality among CAD patients undergoing PCI remains unclear, especially in individuals complicated with diabetes mellitus (DM) or prediabetes (Pre-DM).

Methods: 6,140 patients with CAD undergoing PCI were included in the study and subsequently divided into three groups according to FIB levels (FIB-L, FIB-M, FIB-H). These patients were further grouped by glycemic status [normoglycemia (NG), Pre-DM, DM]. The primary endpoint was all-cause mortality. The secondary endpoint was cardiac mortality.

Results: FIB was positively associated with hemoglobin A1c (HbA1c) and fasting blood glucose (FBG) in CAD patients with and without DM (P < 0.001). During a median follow-up of 5.1 years (interquartile range 5.0-5.2 years), elevated FIB was significantly associated with long-term all-cause mortality (adjusted HR: 1.86; 95% CI 1.28-2.69; P = 0.001) and cardiac mortality (adjusted HR: 1.82; 95% CI 1.15-2.89; P = 0.011). Similarly, patients with DM, but not Pre-DM, had increased risk of all-cause and cardiac mortality compared with NG group (all P < 0.05). When grouped by both FIB levels and glycemic status, diabetic patients with medium and high FIB levels had higher risk of mortality [(adjusted HR: 2.57; 95% CI 1.12-5.89), (adjusted HR: 3.04; 95% CI 1.35-6.82), all P < 0.05]. Notably, prediabetic patients with high FIB also had higher mortality risk (adjusted HR: 2.27; 95% CI 1.01-5.12).

Conclusions: FIB was independently associated with long-term all-cause and cardiac mortality among CAD patients undergoing PCI, especially in those with DM and Pre-DM. FIB test may help to identify high-risk individuals in this specific population.
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http://dx.doi.org/10.1186/s12933-021-01335-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8283976PMC
July 2021

In vitro trypsin digestion and identification of possible cross-linking sites induced by transglutaminase (TGase) of silver carp (Hypophthalmichthys molitrix) surimi gels with different degrees of cross-linking.

Food Chem 2021 Dec 24;364:130443. Epub 2021 Jun 24.

College of Food Science and Technology/National R&D Branch Center for Conventional Freshwater Fish Processing (Wuhan), Huazhong Agricultural University, Wuhan, Hubei Province 430070, PR China. Electronic address:

Surimi gels with different cross-linking degrees (18.52%, 34.67%, 62.87% and 79.11%) were prepared to identify the numbers and locations of lysine residues involved in TGase-induced cross-linking, and to reveal the quantity and location relationships among cross-linking degrees, cross-linking sites and digestion sites by using trypsin digestion, SDS-PAGE and LC-MS/MS methods. The results showed that with the increase in cross-linking degree from 18.52% to 79.11%, 1) the quantity of cross-linking sites gradually increased from 25 sites to 47 sites, 2) the main possible cross-linking domain moved from myosin head to rod, 3) the numbers of digestion sites first decreased from 1262 sites to 1194 sites, and then increased to 1302 sites, 4) the changes in the values of digestion sites were mainly concentrated in myosin rod and it was also the main region of digestion. This study can help exploring the relationship between enzymatic cross-linking and nutritional properties of food.
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http://dx.doi.org/10.1016/j.foodchem.2021.130443DOI Listing
December 2021

Real-world long-term outcomes based on three therapeutic strategies in very old patients with three-vessel disease.

BMC Cardiovasc Disord 2021 06 29;21(1):316. Epub 2021 Jun 29.

Fu Wai Hospital, National Center for Cardiovascular Diseases, Peking Union Medical College and Chinese Academy of Medical Sciences, No. 167, Beilishi Rd, Xicheng District, Beijing, 100037, China.

Background: There are relatively limited data regarding real-world outcomes in very old patients with three-vessel disease (3VD) receiving different therapeutic strategies. This study aimed to perform analysis of long-term clinical outcomes of medical therapy (MT), coronary artery bypass grafting (CABG), and percutaneous coronary intervention (PCI) in this population.

Methods: We included 711 patients aged ≥ 75 years from a prospective cohort of patients with 3VD. Consecutive enrollment of these patients began from April 2004 to February 2011 at Fu Wai Hospital. Patients were categorized into three groups (MT, n = 296; CABG, n = 129; PCI, n = 286) on the basis of different treatment strategies.

Results: During a median follow-up of 7.25 years, 262 deaths and 354 major adverse cardiac and cerebrovascular events (MACCE) occurred. Multivariate Cox analysis showed that the risk of cardiac death was significantly lower for CABG compared with PCI (adjusted hazard ratio [HR] = 0.475, 95% confidence interval [CI] 0.232-0.974, P = 0.042). Additionally, MACCE appeared to show a trend towards a better outcome for CABG (adjusted HR = 0.759, 95% CI 0.536-1.074, P = 0.119). Furthermore, CABG was significantly superior in terms of unplanned revascularization (adjusted HR = 0.279, 95% CI 0.079-0.982, P = 0.047) and myocardial infarction (adjusted HR = 0.196, 95% CI 0.043-0.892, P = 0.035). No significant difference in all-cause death between CABG and PCI was observed. MT had a higher risk of cardiac death than PCI (adjusted HR = 1.636, 95% CI 1.092-2.449, P = 0.017). Subgroup analysis showed that there was a significant interaction between treatment strategy (PCI vs. CABG) and sex for MACCE (P = 0.026), with a lower risk in men for CABG compared with that of PCI, but not in women.

Conclusions: CABG can be performed with reasonable results in very old patients with 3VD. Sex should be taken into consideration in therapeutic decision-making in this population.
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http://dx.doi.org/10.1186/s12872-021-02067-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243749PMC
June 2021

Proteomic profiling and oxidation site analysis of gaseous ozone oxidized myosin from silver carp (Hypophthalmichthys molitrix) with different oxidation degrees.

Food Chem 2021 Nov 6;363:130307. Epub 2021 Jun 6.

College of Food Science and Technology/National R&D Branch Center for Conventional Freshwater Fish Processing (Wuhan), Huazhong Agricultural University, Wuhan, Hubei Province 430070, China; Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Wuhan 430070, China. Electronic address:

Ozone is used to in surimi production and affects the conformation of myosin and gelling properties. Amino acid analysis, SDS-PAGE, in-gel trypsin hydrolysis and LC-MS/MS were used to investigate the effect of the ozone treatment time on the oxidation principle of amino acids to identify the oxidation site and oxidation mechanism of myosin with different oxidation degrees. The results showed that the order of ozonation of amino acids from easy to difficult was tyrosine > cysteine > histidine > proline. The protein structure near the SH1-SH2 region initially changed when the ozone treatment time was 50 s. Prolonging the oxidation to 80 s leads to an irregular distribution of oxidation sites. Ten min of ozone treatment resulted in the aggregation from the SH1 helical region and myosin rod. This study helped to clarify the mechanism of ozone oxidation, thus providing a theoretical basis for producing surimi products of improved quality.
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http://dx.doi.org/10.1016/j.foodchem.2021.130307DOI Listing
November 2021

The Role of Neuroinflammation in Post-traumatic Epilepsy.

Front Neurol 2021 28;12:646152. Epub 2021 May 28.

Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Post-traumatic epilepsy (PTE) is one of the consequences after traumatic brain injury (TBI), which increases the morbidity and mortality of survivors. About 20% of patients with TBI will develop PTE, and at least one-third of them are resistant to conventional antiepileptic drugs (AEDs). Therefore, it is of utmost importance to explore the mechanisms underlying PTE from a new perspective. More recently, neuroinflammation has been proposed to play a significant role in epileptogenesis. This review focuses particularly on glial cells activation, peripheral leukocytes infiltration, inflammatory cytokines release and chronic neuroinflammation occurrence post-TBI. Although the immune response to TBI appears to be primarily pro-epileptogenic, further research is needed to clarify the causal relationships. A better understanding of how neuroinflammation contributes to the development of PTE is of vital importance. Novel prevention and treatment strategies based on the neuroinflammatory mechanisms underlying epileptogenesis are evidently needed.

Search Strategy: Search MeSH Terms in pubmed: "["Epilepsy"(Mesh)] AND "Brain Injuries, Traumatic"[Mesh]". Published in last 30 years. 160 results were founded. Full text available:145 results. Record screened manually related to Neuroinflammation and Post-traumatic epilepsy. Then finally 123 records were included.
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http://dx.doi.org/10.3389/fneur.2021.646152DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8194282PMC
May 2021

Relationship Between High-Sensitivity C-Reactive Protein and Long-Term Outcomes in Elderly Patients With 3-Vessel Disease.

Angiology 2021 Jun 10:33197211021195. Epub 2021 Jun 10.

34736Fuwai Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, People's Republic of China.

The prognostic value of high-sensitivity C-reactive protein (hsCRP) in complex coronary artery disease has not been fully established. We aimed to determine the association between hsCRP and long-term outcomes in elderly patients with 3-vessel disease (TVD). From April 2004 to February 2011, 3069 patients aged ≥65 years with TVD were consecutively enrolled and received medical treatment alone, percutaneous coronary intervention, or coronary artery bypass grafting. The patients were divided into 2 groups according to their hsCRP levels: <3.00 mg/L (62.1%) and ≥3.00 mg/L (37.9%). The mean age was 71 ± 4 years. The high hsCRP group had more risk factors and more frequently received conservative treatment than the low hsCRP group. During a median follow-up period of 6.2 years, elevated hsCRP was significantly associated with increased all-cause death (19.5% vs 29.6%, < .001), cardiac death (9.4% vs 15.2%, = .001), and major adverse cardiovascular and cerebrovascular events (34.1% vs 42.5%, = .001). Multivariable Cox regression analyses revealed that hsCRP was an independent predictor for all of these events. Combining hsCRP with Synergy between PCI with TAXUS and Cardiac Surgery score II further improved the predictive power of the score. The relationship between hsCRP and mortality was relatively consistent across subgroups. Overall, hsCRP could prove useful for risk prediction in elderly patients.
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http://dx.doi.org/10.1177/00033197211021195DOI Listing
June 2021

Accumulation and subcellular distribution of cadmium in rygegrass induced by TL-F2 and TL-F3.

Int J Phytoremediation 2021 Jun 8:1-8. Epub 2021 Jun 8.

Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei, China.

Although plant growth-promoting fungi can greatly accelerate the ryegrass bioaccumulation of cadmium (Cd), the underlying mechanisms are not yet well documented. Therefore, we performed a 20-days hydroponic experiment to investigate the effects of TL-F2 ( TL-F2) and TL-F3 ( TL-F3) on accumulation/subcellular distribution of Cd by annual ryegrass Dongmu 70 at different Cd concentrations (0, 2.5, and 5 mg L). Results indicated that both fungal strains promoted ryegrass biomass/growth by about 60%. Furthermore, we found that ryegrass roots (17.8-37.1 μg pot) had a significantly higher capability for Cd uptake than the shoots (1.66-5.45 μg pot) ( < 0.05). Of total Cd in ryegrass plants, 44-67% was in soluble form, 24-37% was in cell wall, and 8.5-25.5% was in organelles. Compared with non-fungus ryegrass, cell wall and soluble Cd fractions in fungus-inoculated roots increased and decreased by 13.5-44% and 21.5-26.4%, respectively. Besides, fungus inoculation generally increased the content of cell wall and soluble Cd fractions in ryegrass shoots. Altogether, the study concludes that inoculation of fungus in ryegrass is a promising approach to improve phytoremediation of Cd contaminated environments. Previous study by Han (2018) examined the resistance of ryegrass plant to Cd stress after its inoculation with . In this study, using a hydroponic experiment, we examined the effects of co-application of two species of fungi. i.e. TL-F2 and TL-F3 on ryegrass growth/biomass, Cd absorption by ryegrass shoots and roots, and subcellular distribution of Cd in ryegrass roots and shoots.
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http://dx.doi.org/10.1080/15226514.2021.1932734DOI Listing
June 2021

Does Percutaneous Coronary Intervention on Off Days have an Effect on Long-term Prognosis in Patients with Coronary Artery Disease in China?

Biomed Environ Sci 2021 May;34(5):387-394

Department of Cardiology, Fuwai Hospital, Chinese Academy of Medical Sciences, Beijing 100037, China.

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http://dx.doi.org/10.3967/bes2021.051DOI Listing
May 2021

Role of epigallocatechin gallate in collagen hydrogels modification based on physicochemical characterization and molecular docking.

Food Chem 2021 Oct 12;360:130068. Epub 2021 May 12.

College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China; The Sub Center (Wuhan) of National Technology and R&D of Staple Freshwater Fish Processing, Wuhan 430070, PR China. Electronic address:

Collagen Type I derived from fish is mainly limited by its poor physicochemical properties for further applications. In this study, we developed epigallocatechin gallate (EGCG) cross-linked collagen hydrogels (EC hydrogels) to realize physicochemical improvements, basing on the interaction mechanism between collagen and EGCG. The integrity of collagen framework with slight secondary structure change in the presence of EGCG was confirmed. The stronger stability of collagen fibrils was proved by slower swelling ratio, declined enzymatic degradation, improved thermal analysis and mechanical test due to EGCG modification. To illustrate the potential mechanism between collagen and EGCG, molecular docking was used to identify both covalent (CN bond, between lysine of collagen and C-ring B of EGCG) and non-covalent bonds (hydrogen bond and hydrophobic interaction) within in EC hydrogel. Taken together, this work would offer some insights into the further study about the interaction between EGCG and collagen.
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http://dx.doi.org/10.1016/j.foodchem.2021.130068DOI Listing
October 2021

The use of different sublethal endpoints to monitor atrazine toxicity in nematode Caenorhabditis elegans.

Chemosphere 2021 Jul 3;274:129845. Epub 2021 Feb 3.

Nanjing Institute of Environmental Science, Ministry of Ecology and Environment, Nanjing, 210042, China. Electronic address:

In this work, Caenorhabditis elegans was employed as an in vivo model to determine the toxic effects of atrazine at different concentrations. After the exposure period from the larval stage L1 to adulthood day 1, atrazine (10 mg/L) significantly decreased the body length and lifespan of nematodes. In addition, exposure to ≥0.01 mg/L atrazine remarkably increased the intestinal reactive oxygen species (ROS) levels and reduced locomotion behavior of nematodes, while exposure to ≥ 1 mg/L atrazine decreased the brood size of nematodes. Moreover, atrazine (0.001-0.1 mg/L) upregulated the expression levels of hsp-6::GFP and hsp-6/60 in nematodes, indicating the activation of mitochondrial unfolded protein response (mtUPR). On the contrary, atrazine (1-10 mg/L) downregulated the expression levels of hsp-6::GFP and hsp-6/60 in nematodes. Furthermore, mtUPR induction governed by the RNAi knockdown of atfs-1 could increase the vulnerability of nematodes against atrazine toxicity. Overall, our findings highlighted the dynamic responses of nematodes toward different concentrations of atrazine, which could be monitored using different sublethal endpoints as bioindicators.
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http://dx.doi.org/10.1016/j.chemosphere.2021.129845DOI Listing
July 2021

Characterizing the seizure onset zone and epileptic network using EEG-fMRI in a rat seizure model.

Neuroimage 2021 08 2;237:118133. Epub 2021 May 2.

Bioland Laboratory, Guangzhou Regenerative Medicine and Health Guangdong Laboratory, Guangzhou, China; Laboratory of Brain Disorders, Ministry of Science and Technology, Collaborative Innovation Center for Brain Disorders, Capital Medical University, Beijing Institute of Brain Disorders, Beijing, China; Neuroelectrophysiological Laboratory, Xuanwu Hospital, Capital Medical University, Beijing, China. Electronic address:

Accurate epileptogenic zone (EZ) or seizure onset zone (SOZ) localization is crucial for epilepsy surgery optimization. Previous animal and human studies on epilepsy have reported that changes in blood oxygen level-dependent (BOLD) signals induced by epileptic events could be used as diagnostic markers for EZ or SOZ localization. Simultaneous electroencephalography and functional magnetic resonance imaging (EEG-fMRI) recording is gaining interest as a non-invasive tool for preoperative epilepsy evaluation. However, EEG-fMRI studies have reported inconsistent and ambiguous findings. Therefore, it remains unclear whether BOLD responses can be used for accurate EZ or SOZ localization. In this study, we used simultaneous EEG-fMRI recording in a rat model of 4-aminopyridine-induced acute focal seizures to assess the spatial concordance between individual BOLD responses and the SOZ. This was to determine the optimal use of simultaneous EEG-fMRI recording in the SOZ localization. We observed a high spatial consistency between BOLD responses and the SOZ. Further, dynamic BOLD responses were consistent with the regions where the seizures were propagated. These results suggested that simultaneous EEG-fMRI recording could be used as a noninvasive clinical diagnostic technique for localizing the EZ or SOZ and could be an effective tool for mapping epileptic networks.
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http://dx.doi.org/10.1016/j.neuroimage.2021.118133DOI Listing
August 2021

The effect of cross-linking degree on physicochemical properties of surimi gel as affected by MTGase.

J Sci Food Agric 2021 Apr 30. Epub 2021 Apr 30.

College of Food Science and Technology/National R&D Branch Center for Conventional Freshwater Fish Processing (Wuhan), Huazhong Agricultural University, Wuhan, PR China.

Background: The transglutaminase-induced cross-linking reaction can enhance the textural properties of surimi gels. However, when the cross-linking degree exceeds a certain range, surimi gels become brittle, giving the gel a special mouthfeel. Little information is to be found regarding the brittleness of surimi gel and its formation mechanism. The present study aimed to investigate the effects of cross-links on physicochemical properties of surimi gel and to analyze the reason for the textural variation of surimi gels regulated by cross-links.

Results: When the cross-linking degree was lower than about 30%, the surimi gel could not be fractured and the surimi gel was mushy. When the cross-linking degree was around 40-55%, surimi gels without microbial transglutaminase (MTGase) presented an elastic texture, while surimi gels with MTGase displayed a tough texture. When the cross-linking degrees were 64.1% and 76.5%, surimi gels became brittle. Water holding capacity decreased with the increase in cross-linking degree and the cross-links released some combined water to free water. With the increase in cross-links, the pore equivalent diameter and the fractal dimension first decreased and then increased. When the cross-linking degree exceeded approximately 50%, hydrophobic interaction increased, consistent with the exposure of hydrophobic residues and the decrease in the water gap between proteins.

Conclusion: The changes in gel texture were related to micro-network structure, water status and microenvironment of proteins. It was demonstrated that the texture of slowly heated surimi-based products could be regulated by controlling the TGase-induced cross-links. © 2021 Society of Chemical Industry.
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http://dx.doi.org/10.1002/jsfa.11274DOI Listing
April 2021

A Follow-Up Investigation of Mental Health Among Discharged COVID-19 Patients in Wuhan, China.

Front Public Health 2021 12;9:640352. Epub 2021 Apr 12.

Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing, China.

To understand the mental health status and its risk factors among discharged COVID-19 patients during the first month of centralized quarantine and the subsequent home isolation. The scales of the Insomnia Severity Index (ISI), General Anxiety Disorder (GAD-7), and Patient Health Questionnaire (PHQ-9) were used to measure the symptoms of insomnia, anxiety, and depression in 782 COVID-19 patients during the first month of centralized quarantine (March 16 to 26, 2020) and then during home isolation (April 3 to 10, 2020). During the centralized quarantine, the prevalence rates of insomnia, anxiety, and depressive symptoms were 44.37, 31.59, and 27.62%, respectively, and those during the home isolation decreased significantly at 27.11, 17.26, and 16.11%, respectively. In both waves, women showed a higher prevalence of symptoms of poor mental health compared to men, and middle-aged (40-59 years old) and elderly (≥60 years old) showed a higher risk of symptoms of poor mental health compared to the younger. In addition, the severity of COVID-19 revealed no significant relationship to symptoms of poor mental health, whereas, the interaction analysis revealed that those with other underlying diseases showed more symptoms of poor mental health during the centralized quarantine and a greater decrease during the follow-up home isolation. The discharged COVID-19 patients suffered from mental health problems such as, insomnia, depression, and anxiety, and this was especially so for women, the middle-aged and elderly, and those with underlying diseases, but along with the rehabilitation and the environmental change from centralized quarantine to home isolation, all the mental symptoms were significantly alleviated. Based on a follow-up investigation, the current results provide critical evidence for mental health and early rehabilitation upon the discharged COVID-19 patients.
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http://dx.doi.org/10.3389/fpubh.2021.640352DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071993PMC
May 2021

Insights into ion imprinted membrane with a delayed permeation mechanism for enhancing Cd selective separation.

J Hazard Mater 2021 08 31;416:125772. Epub 2021 Mar 31.

Key Laboratory of Jiangxi Province for Persistent Pollutants Control and Resources Recycle, Nanchang Hangkong University, Nanchang 330063, China. Electronic address:

Ion imprinted polymers exhibit great potential in ion separation from wastewater. However, the difficulty of ion separation by membrane is proverbial, which severely restricts the application of membrane in metal resource recovery from industrial wastewater. Herein, a rational molecular-level design approaches for membrane fabrication was developed to modify a layer of ion imprinted polymer onto the PVDF membrane. Batch rebind and permeation experiments suggest that specific host-guest binding sites had been fabricated along the membrane pore in ion imprinted membranes (IIM). A higher monomer dose leads to a higher rejection of Cd, and the more bind sites in IIM. The binding of IIM to Cd was 1.84 times that of non-ion imprinted membranes (NIM). Permselectivity factors (γ) of IIM are larger than 5.39 in mixture ions solutions. Chemical characterization and density functional theory (DFT) calculation reveal that the Cd recognition sites of functional groups are C-S and C˭S. Cd mass transport in IIM suggest that the imprint effects provide a binding force that would delay Cd to permeate through IIM, so as to selectively separate Cd with other ions. The imprint effects may enlighten a novel molecular-level design approaches for membrane fabrication to enhance the selectivity of ion-ion.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125772DOI Listing
August 2021

To assess the effective and safety of compound glutamine entersoluble capsules in irritable bowel syndrome: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 Mar;100(10):e25098

Pharmacy Department, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China.

Background: Irritable bowel syndrome (IBS) is one the common medical condition of functional GI disorder (FGD) characterized by bowel-related symptoms without other organic gastrointestinal (GI) disease. Compound Glutamine Entersoluble Capsules(CGEC),a compound preparation in which each capsule contains 120 mg L-glutamine, 50 mg ginseng, 50 mg licorice, 50 mg Atractylodes macrocephala and 50 mg Poria cocos, have been reported the efficacy of CGEC for patients with IBS in improving the clinical symptoms and quality of patients' life. However, there is no a systematic review related to CGEC for IBS to this day. In this study, we will systematically evaluate the effectiveness and safety of CGEC in the treatment of IBS-D with a meta-analysis method, so as to provide a solid evidence for clinical practice.

Methods: In this study, a literature search was performed by using the Chinese and English databases, which include PubMed, Embase, MEDLINE, Cochrane Library Central Register of Controlled Trials, China National Knowledge Infrastructure (CNKI) database, Wanfang Data Knowledge Service Platform, the VIP information resource integration service platform (cqvip), China Biology Medicine Disc (Sino Med),and the Chinese Clinical Trial Registry (ChiCTR), to find the related literature of CGEC in the treatment of IBS published from the inception date of each predefined database upto January 2021. The evaluation of the risk of bias for eligible studies will be performed by two investigators. Data synthesis will be performed by RevMan 5.4 software. Heterogeneity between studies can be assessed by a heterogeneity X2 test. The degree of heterogeneity among multiple included studies can be measured by I2. The stability of systematic review or meta-analysis outcomes will be evaluated by Sensitivity analysis. Reporting bias will be evaluated by funnel plot. Finally, The Grading of Recommendations Assessment, Development and Evaluation (GRADE) will be used to assess the quality of evidence obtained.

Results: The results of this study will be published in a peer-reviewed journal.

Conclusion: Whether it is the effectiveness and safety of CGEC in the treatment of IBS will be judged in the result of this systematic review.
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http://dx.doi.org/10.1097/MD.0000000000025098DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969248PMC
March 2021

The Association of Socioeconomic Status with the Burden of Cataract-related Blindness and the Effect of Ultraviolet Radiation Exposure: An Ecological Study.

Biomed Environ Sci 2021 Feb;34(2):101-109

Department of Environmental Health, School of Public Health, China Medical University, Shenyang 110122, Liaoning, China.

Objective: To assess the association of socioeconomic status with the burden of cataract blindness in terms of year lived with disability (YLD) rates and to determine whether ultraviolet radiation (UVR) levels modify the effect of socioeconomic status on this health burden.

Methods: National and subnational age-standardized YLD rates associated with cataract-related blindness were derived from the Global Burden of Disease (GBD) study 2017. The human development index (HDI) from the Human Development Report was used as a measure of socioeconomic status. Estimated ground-level UVR exposure was obtained from the Ozone Monitoring Instrument (OMI) dataset of the National Aeronautics and Space Administration (NASA).

Results: Across 185 countries, socioeconomic status was inversely associated with the burden of cataract blindness. Countries with a very high HDI had an 84% lower age-standardized YLD rate [95% confidence interval ( ): 60%-93%, < 0.001] than countries with a low HDI; for high-HDI countries, the proportion was 76% (95% : 53%-88%, < 0.001), and for medium-HDI countries, the proportion was 48% (95% : 15%-68%, = 0.010; for trend < 0.001). The interaction analysis showed that UVR exposure played an interactive role in the association between socioeconomic status and cataract blindness burden ( value for interaction = 0.047).

Conclusion: Long-term high-UVR exposure amplifies the association of poor socioeconomic status with the burden of cataract-related blindness. The findings emphasize the need for strengthening UVR exposure protection interventions in developing countries with high-UVR exposure.
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http://dx.doi.org/10.3967/bes2021.015DOI Listing
February 2021

Short-chain fatty acids contribute to neuropathic pain via regulating microglia activation and polarization.

Mol Pain 2021 Jan-Dec;17:1744806921996520

Department of Anesthesiology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, China.

Microglia activation and subsequent pro-inflammatory responses play a key role in the development of neuropathic pain. The process of microglia polarization towards pro-inflammatory phenotype often occurs during neuroinflammation. Recent studies have demonstrated an active role for the gut microbiota in promoting microglial full maturation and inflammatory capabilities via the production of Short-Chain Fatty Acids (SCFAs). However, it remains unclear whether SCFAs is involved in pro-inflammatory/anti-inflammatory phenotypes microglia polarization in the neuropathic pain. In the present study, chronic constriction injury (CCI) was used to induce neuropathic pain in mice, the mechanical withdrawal threshold, thermal hyperalgesia were accomplished. The levels of microglia markers including ionized calcium-binding adaptor molecule 1 (Iba1), cluster of differentiation 11b (CD11b), pro-inflammatory phenotype markers including CD68, interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and anti-inflammatory phenotype markers including CD206, IL-4 in the hippocampus and spinal cord were determined on day 21 after CCI. The results showed that CCI produced mechanical allodynia and thermal hyperalgesia, and also increased the expressions of microglia markers (Iba1, CD11b) and pro-inflammatory phenotype markers (CD68, IL-1β, and TNF-α), but not anti-inflammatory phenotype marker (CD206, IL-4) in the hippocampus and spinal cord, accompanied by increased SCFAs in the gut. Notably, antibiotic administration reversed these abnormalities, and its effects was also bloked by SCFAs administration. In conclusion, data from our study suggest that CCI can lead to mechanical and thermal hyperalgesia, while SCFAs play a key role in the pathogenesis of neuropathic pain by regulating microglial activation and subsequent pro-inflammatory phenotype polarization. Antibiotic administration may be a new treatment for neuropathic pain by reducing the production of SCFAs and further inhibiting the process of microglia polarization.
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http://dx.doi.org/10.1177/1744806921996520DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7925956PMC
February 2021

Effect of Baseline Thrombocytopenia on Long-Term Outcomes in Patients With Acute ST-Segment Elevated Myocardial Infarction - A Large Propensity Score-Matching Analysis From the China Acute Myocardial Infarction (CAMI) Registry.

Circ J 2021 01 14;85(2):150-158. Epub 2021 Jan 14.

Department of Cardiology, Fuwai Hospital, Chinese Academy of Medical Sciences.

Background: Data on the association of baseline thrombocytopenia (TP) with long-term outcomes of patients with acute ST-segment elevated myocardial infarction (STEMI) are still limited.Methods and Results:A total of 16,957 consecutive cases of patients with STEMI from multiple centers that participated in the China Acute Myocardial Infarction (CAMI) registry were included in this study. Two-year clinical outcomes were evaluated between patients with TP and those with a normal platelet count (PLT). Cases coexisting with baseline TP accounted for 2.1%. The rates of 2-year all-cause death (21.4% and 11.4%, P<0.001) and major adverse cardiovascular and cerebrovascular events (MACCE) (23.6% and 13.9%, P<0.001) were significantly higher in cases with TP, compared with the normal PLT group. After multivariate adjustment, compared with the control, cases with TP were not independently associated with 2-year all-cause death (HR: 1.21; 95% CI: 0.96-1.52; P=0.110) and MACCE (HR: 1.18; 95% CI: 0.95-1.47; P=0.132). After propensity score matching (PSM), the rates of 2-year all-cause death and MACCE were similar between the 2 groups (20.7% and 17.9%, P=0.317; 23.0% and 19.9%, P=0.288). Multivariable adjustment after PSM showed baseline TP was not independently associated with all-cause death (HR: 1.21; 95% CI: 0.88-1.67; P=0.240) and MACCE (HR: 1.21; 95% CI: 0.89-1.63; P=0.226).

Conclusions: Patients with STEMI and baseline TP had higher rates of all-cause death and MACCE; however, baseline TP was not independently associated with 2-year adverse outcomes in patients with STEMI after multivariate adjustment and controlling for baseline differences.
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http://dx.doi.org/10.1253/circj.CJ-20-0781DOI Listing
January 2021

Theoretical mechanistic study on the reaction of the methoxymethyl radical with nitrogen dioxide.

J Mol Model 2021 Jan 7;27(2):18. Epub 2021 Jan 7.

School of Chemical Engineering, Northwest University, Xi'an, 710069, China.

Mechanism of the reaction of CHOCH with NO is explored theoretically at the M062X/MG3S and G4 levels. The calculated results indicate two stable association intermediates, CHOCHNO (IM1) and CHOCHONO (IM2), which can be produced by the attack of the nitrogen or oxygen atom of NO to terminal carbon atom of CHOCH without barrier involved. IM2 is found to take trans (IM2a)-cis (IM2b) conversion and isomerization to IM1, with the following stability order IM2a > IM2b > IM1. Starting from IM2a, the most feasible pathway is the direct O-NO bond cleavage leading to P1 (CHOCHO + NO) or the H-shift and O-NO bond rupture to produce P2 (CHOCHO + HNO), both of which have comparable contribution to the title reaction. There also involves an H-transfer from the methyl group of IM2a to the N atom with the simultaneous dissociations of the C-O and O-N bonds to produce P4 (2CHO + HNO). In addition, another dissociation pathway is open to IM2b which decompose to P5 (CHO + CHONO) by the O-N and C-O bond scissions and the recombination of CHO and NO. Because all the intermediates and transition states involved in the above pathways lie below reactants, the CHOCH + NO reaction is expected to be rapid. Subsequent dissociation of IM1 and direct H-abstraction between CHOCH and NO are kinetically almost inhibited due to significantly high barriers. The present results can lead us to deeply understand the mechanism of the title reaction and may be helpful for understanding NO combustion chemistry.
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http://dx.doi.org/10.1007/s00894-020-04657-1DOI Listing
January 2021

Double-crosslinked effect of TGase and EGCG on myofibrillar proteins gel based on physicochemical properties and molecular docking.

Food Chem 2021 May 19;345:128655. Epub 2020 Nov 19.

College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, PR China; Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Wuhan 430070, PR China. Electronic address:

The aim of this study was to determine the influence and mechanism of combining EGCG with TGase on properties of myofibrillar protein (MP) gel. A double-crosslinked effect was observed when EGCG and TGase were added into MP gel. Breaking force, deformation, water holding capacity and hardness of double-crosslinked MP gel increased by 25.3 ± 3.0 g, 0.5 ± 0.3 mm, 1.76 ± 0.4% and 34.11 ± 2.56 g, compared with those of TGase induced gel. Light microscopy and low-field nuclear magnetic resonance results indicated with EGCG content increasing, pores and structure of double-crosslinked gels became smaller and denser, T decreased from 266.162 ms to 252.845 ms and its proportion increased from 94.103% to 96.956%. Molecular docking illustrated covalent and non-covalent interactions between EGCG and myosin heavy chain Ⅱ A, and confirmed TGase catalytic mechanism with myosin heavy chain Ⅱ A as substrate. Therefore the mixture of EGCG and TGase could be used as novel cross-linker in surimi.
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http://dx.doi.org/10.1016/j.foodchem.2020.128655DOI Listing
May 2021

Contribution of DNA methyltransferases to spared nerve injury induced depression partially through epigenetically repressing Bdnf in hippocampus: Reversal by ketamine.

Pharmacol Biochem Behav 2021 01 24;200:173079. Epub 2020 Nov 24.

Department of Anesthesiology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, China. Electronic address:

Long-lasting pain can induce depression, which seriously affects life quality of the patients, but little is known about the underlying mechanism. Chronic neuropathic pain can modulate DNA methylation in target genes related to neuroplasticity and mood regulation, which was induced by DNA methyltransferases (DNMTs). Methylation changes of brain-derived neurotrophic factor (Bdnf) in the hippocampus are critical for neuropathic pain and depression. Thus, we hypothesized that DNMTs are required for depression genesis, probably by repressing hippocampus Bdnf gene expression in rats with neuropathic pain, which can be rescued by ketamine. In the present study, rats were randomly subjected to spared nerve injury (SNI) or sham surgery. SNI upregulated DNMTs and downregulated Bdnf and exon I in the hippocampus and induced depression behaviors, whereas blocking the upregulation of DNMTs with RG108 alleviated SNI-induced depression by up-regulation of the expression of Bdnf and exon I. In addition, we showed that a single dose of ketamine could ameliorate SNI-induced depression-like behaviors, which was related to normalization of DNMTs and Bdnf. In conclusion, our study suggested that DNMTs-induced decreased expression of Bdnf may induce the comorbid of pain and depression, which can be prevented by ketamine.
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http://dx.doi.org/10.1016/j.pbb.2020.173079DOI Listing
January 2021

Association of and Gene Polymorphisms with Major Adverse Cardiac and Cerebrovascular Events in Patients with Three-Vessel Disease.

Hum Gene Ther 2021 Jun 22;32(11-12):581-588. Epub 2021 Jan 22.

State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Three-vessel disease (TVD) is a severe coronary heart disease (CHD) with poor prognosis. Niemann-Pick C1-like 1 () is a transporter protein for exogenous cholesterol absorption, and 3-hydroxy-3-methylglutaryl-coenzyme A reductase () is a rate-limiting enzyme for cholesterol synthesis. We aimed to investigate the association between and gene polymorphisms and major adverse cardiac and cerebrovascular events (MACCE) in patients with TVD. A total of 342 TVD patients were consecutively enrolled and followed up for 1-year MACCE (a composite of all-cause death, myocardial infarction, revascularization, readmission, and stroke) as TVD event group, and 344 patients without CHD were control group. Four single-nucleotide polymorphisms (SNPs), rs11763759, rs4720470, rs2072183, and rs2073547, on gene and four SNPs, rs12916, rs2303151, rs2303152, and rs4629571, on gene were genotyped. Multivariate logistic regression analysis showed that rs4720470 of was associated with higher risk of TVD with MACCE in codominant model (odds ratio [OR]: 1.315; 95% confidence intervals [CI]: 1.007-1.716,  = 0.044), and that rs2303151 of was associated with higher in recessive (OR: 3.383; 95% CI: 1.040-10.998,  = 0.043) and codominant (OR: 1.458; 95% CI: 1.038-2.047,  = 0.030) model, respectively. Patients with both variant rs4720470 in codominant model and variant rs2303151 in recessive model related to a higher risk (OR: 6.772, CI: 1.338-34.280;  = 0.021). We reported for the first time that the rs4720470 on gene and rs2303151 on gene were associated with risk of 1-year MACCE in TVD.
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http://dx.doi.org/10.1089/hum.2020.229DOI Listing
June 2021

Interaction of myofibrillar proteins and epigallocatechin gallate in the presence of transglutaminase in solutions.

Food Funct 2020 Nov;11(11):9560-9572

College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, P. R. China.

The rheological behavior, assembly measurements, thermal stability, molecular conformation, and molecular interactions of myofibrillar proteins (MP) modified by transglutaminase (TGase) and epigallocatechin-3-gallate (EGCG) were investigated. Fluorescence and ultraviolet spectra showed that TGase and ECCG quenched the endogenous fluorescence of MP and improved the exposure of chromophoric and hydrophobic groups of MP caused by unraveling the tertiary structure. TGase and EGCG treatment increased the α-helix and β-turn contents and the structure became more ordered. It also increased the thermal stability of MP and the storage modulus of MP gels as reflected in the rheological and thermal properties tests. Meanwhile, changes in SDS-PAGE, FT-IR, and sulfhydryl contents showed that TGase increased the disulfide bond contents, whereas it decreased after EGCG was added, suggesting that EGCG could react with MP via non-covalent and covalent interactions. Assembly measurements illustrated that TGase increased the turbidity and particle size of MP, while TGase and EGCG treatment accelerated MP aggregation and it was associated with the increase in the EGCG concentration, which was further confirmed by SEM and AFM. This work could contribute new insights into the synergistic effect of TGase and EGCG on modifying MP and guide for its application in surimi processing.
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http://dx.doi.org/10.1039/d0fo02294bDOI Listing
November 2020

Dialdehyde modified cellulose nanofibers enhanced the physical properties of decorative paper impregnated by aldehyde-free adhesive.

Carbohydr Polym 2020 Dec 16;250:116941. Epub 2020 Aug 16.

Department of Chemical Engineering, University of New Brunswick, Fredericton, NBE3B5A3, Canada. Electronic address:

Impregnated decorative paper was an important wood-based panel finishing material. However, traditional impregnated decorative paper was impregnated with melamine-formaldehyde resin, which will release formaldehyde and harm the human health. To solve this problem, small molecule polyacrylate-polyethylene glycol (PEG) adhesive was used to achieve the non-formaldehyde addition of the impregnation system. The dialdehyde modified CNF (D-CNF), modified by sodium periodate (NaIO), and triethylenediamine were introduced to enhance the surface properties of the impregnated decorative paper. The results showed that the incorporation of D-CNF and triethylenediamine imparted excellent physical strength and surface properties to impregnated decorative paper. When the dosage of 0.3 wt% D-CNF and 3 mL/100 g triethylenediamine in the compound emulsion, the hardness, abrasion resistant value and surface bonding strength of impregnated decorative paper adhered fiberboard reached 3H, 330 r of damage and 1.13 MPa, respectively. Thus, it could be effectively used for making high-performance formaldehyde-free impregnated decorative paper.
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http://dx.doi.org/10.1016/j.carbpol.2020.116941DOI Listing
December 2020

Effect of high intensity ultrasound on gelation properties of silver carp surimi with different salt contents.

Ultrason Sonochem 2021 Jan 1;70:105326. Epub 2020 Sep 1.

College of Food Science and Technology, Huazhong Agricultural University/National R and D Branch Center for Conventional Freshwater Fish Processing (Wuhan), Wuhan, Hubei Province 430070, PR China; Key Laboratory of Environment Correlative Dietology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei Province 430070, PR China. Electronic address:

Surimi from silver carp with different salt contents (0-5%) was obtained treated by high intensity ultrasound (HIU, 100 kHz 91 W·cm). The gelation properties of samples were evaluated by puncture properties, microstructures, water-holding capacity, dynamic rheological properties and intermolecular interactions. As the salt content increased from 0 to 5%, gel properties of surimi without HIU significantly improved. For samples with low-salt (0-2% NaCl) content, HIU induced obvious enhancement in breaking force and deformation. HIU promoted the protein aggregation linked by SS bonds, hydrophobic interactions and non-disulfide covalent bonds in surimi gels with low-salt content. Moreover, microstructures of HIU surimi gels with low-salt content were more compact than those of the corresponding control samples. HIU also improved the gelation properties of surimi with 3% NaCl to an extent. However, for high-salt (4-5% NaCl) samples, HIU decreased the breaking force and deformation of surimi gels due to the degradation of proteins suggested by increased TCA-soluble peptides. In conclusion, HIU effectively improved the gelation properties of surimi with low-salt content (0-2% NaCl), but was harmful for high-salt (4-5% NaCl) surimi. This might provide the theoretical basis for the production of low-salt surimi gels.
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http://dx.doi.org/10.1016/j.ultsonch.2020.105326DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7786557PMC
January 2021

Suppressing the Radiation-Induced Corrosion of Bismuth Nanoparticles for Enhanced Synergistic Cancer Radiophototherapy.

ACS Nano 2020 10 29;14(10):13016-13029. Epub 2020 Sep 29.

CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics and National Center for Nanoscience and Technology, Chinese Academy of Sciences, Beijing 100049, China.

The level of tumor killing by bismuth nanoparticles (BiNPs) as radiosensitizers depends strongly on the powerful particle-matter interaction. However, this same radiation leads to the structural damage in BiNPs, consequently weakening their specific physicochemical properties for radiosensitization. Herein, we studied the radiation-induced corrosion behavior of BiNPs and demonstrated that these damages were manifested by the change in their morphology and crystal structure as well as self-oxidation at their surface. Furthermore, artificial heterostructures were created with graphene nanosheets to greatly suppress the radiation-induced corrosion in BiNPs and enhance their radiocatalytic activity for radiotherapy enhancement. Such a nanocomposite allows the accumulation of overexpressed glutathione, a natural hole scavenger, at the reaction interfaces. This enables the rapid removal of radiogenerated holes from the surface of BiNPs and minimizes the self-radiooxidation, therefore resulting in an efficient suppression of radiation corrosion and a decrease of the depletion of reactive oxygen species (ROS). Meanwhile, the radioexcited conduction band electrons react with the high-level HO within cancer cells to yield more ROS, and the secondary electrons are trapped by HO molecules to produce hydrated electrons capable of reducing a highly oxidized species such as cytochrome . These radiochemical reactions together with hyperthermia can regulate the tumor microenvironment and accelerate the onset of cellular redox disequilibrium, mitochondrial dysfunction, and DNA damage, finally triggering tumor apoptosis and death. The current work will shed light on radiosensitizers with an enhanced corrosion resistance for controllable and synergistic radio-phototherapeutics.
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http://dx.doi.org/10.1021/acsnano.0c04375DOI Listing
October 2020

Comparison of Percutaneous Coronary Intervention, Coronary Artery Bypass Grafting and Medical Therapy in Non-ST Elevation Acute Coronary Syndrome Patients With 3-Vessel Disease.

Circ J 2020 09 26;84(10):1718-1727. Epub 2020 Aug 26.

Fu Wai Hospital, National Center for Cardiovascular Diseases, Peking Union Medical College & Chinese Academy of Medical Sciences.

Background: The aim of this study is to compare the long-term prognosis of non-ST elevation acute coronary syndrome (NSTE-ACS) patients with 3-vessel disease (3VD) who underwent percutaneous coronary intervention (PCI), coronary artery bypass grafting (CABG) or medical therapy (MT).Methods and Results:Overall, 3,928 NSTE-ACS patients with 3VD were consecutively enrolled from April 2004 to February 2011 at Fu Wai Hospital. Patients were followed up for a median of 7.5 years, and were divided into PCI, CABG or MT groups according to their treatment. Compared with patients undergoing PCI, CABG patients had lower rates of myocardial infarction (MI), unplanned revascularization, major adverse cardiovascular and cerebrovascular events (MACCE) and a higher rate of stroke (all P<0.05). Compared with MT, PCI and CABG had lower incidences of all adverse outcomes (all P<0.05), except for a similar rate of stroke between PCI and MT. Kaplan-Meier analysis showed similar results. After adjusting for confounders, CABG was independently associated with a lower risk of cardiac death, revascularization and MACCE compared with PCI (all P<0.05). Compared with MT, PCI reduced long-term risk of death, whereas CABG reduced long-term risk of death, revascularization and MACCE events (all P<0.05).

Conclusions: In NSTE-ACS patients with 3VD, CABG is independently associated with a lower risk of long-term cardiac death, revascularization and MACCE compared with PCI. Patients who received MT alone had the highest risk of long-term MACCE.
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http://dx.doi.org/10.1253/circj.CJ-20-0300DOI Listing
September 2020

Pepsin Digestion Characteristics of Silver Carp () Surimi Gels with Different Degrees of Cross-Linking Induced by Setting Time and Microbial Transglutaminase.

J Agric Food Chem 2020 Aug 23;68(31):8413-8430. Epub 2020 Jul 23.

College of Food Science and Technology/National R&D Branch Center for Conventional Freshwater Fish Processing (Wuhan), Huazhong Agricultural University, Wuhan, Hubei Province 430070, P. R. China.

Surimi gels are favored for their abundant proteins and unique taste. In this study, the pepsin digestion behaviors of surimi gels with different degrees of cross-linking induced by microbial transglutaminase (MTGase) and different setting times were investigated. For gels without (CK group) and with (TG group) MTGase, the slowest digestion rate ( = 20.13 and 79.19 min for CK and TG group, respectively), the least amino acid concentration (5.32 and 3.73 μmol/mL for CK and TG group, respectively), and the peptide amounts (1355 and 1788 for CK and TG group, respectively) were obtained at a moderate setting time (1-4 h) with the finest microstructure. However, the excessive setting time (8-12 h) formed an inhomogenous network, which accelerated the hydrolysis of gel proteins ( = 9.40 and 52.33 min for CK and TG group, respectively) and produced more amino acids (6.63 and 5.15 μmol/mL for CK and TG group, respectively) and peptide amounts (1644 and 2143 for CK and TG group, respectively). The above results also demonstrated that the presence of MTGase strengthened the compactness of gels as well as slowed down the digestion process with the release of less amino acids but more peptides. A large proportion of unique peptides were from the tail domain of myosin heavy chain. The discrepancy in bioactive peptides between different gels might be reduced in the subsequent intestinal digestion according to the methods, demonstrating the diminished difference in the gastrointestinal digestion process in the aspect of releasing functional peptides. This study provides the theoretical basis and guideline in the field of gelation food digestion and surimi food industry to produce healthier surimi-based food.
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http://dx.doi.org/10.1021/acs.jafc.0c03014DOI Listing
August 2020
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