Publications by authors named "Rozina Kousar"

11 Publications

  • Page 1 of 1

Anti-bacterial and wound healing-promoting effects of zinc ferrite nanoparticles.

J Nanobiotechnology 2021 Feb 5;19(1):38. Epub 2021 Feb 5.

Department of Biomedical Sciences, Pak-Austria Fachhochschule: Institute of Applied Sciences and Technology, Haripur, 22620, Pakistan.

Background: Increasing antibiotic resistance continues to focus on research into the discovery of novel antimicrobial agents. Due to its antimicrobial and wound healing-promoting activity, metal nanoparticles have attracted attention for dermatological applications. This study is designed to investigate the scope and bactericidal potential of zinc ferrite nanoparticles (ZnFeO NPs), and the mechanism of anti-bacterial action along with cytocompatibility, hemocompatibility, and wound healing properties.

Results: ZnFeO NPs were synthesized via a modified co-precipitation method. Structure, size, morphology, and elemental compositions of ZnFeO NPs were analyzed using X-ray diffraction pattern, Fourier transform infrared spectroscopy, and field emission scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy. In PrestoBlue and live/dead assays, ZnFeO NPs exhibited dose-dependent cytotoxic effects on human dermal fibroblasts. In addition, the hemocompatibility assay revealed that the NPs do not significantly rupture red blood cells up to a dose of 1000 µg/mL. Bacterial live/dead imaging and zone of inhibition analysis demonstrated that ZnFeO NPs showed dose-dependent bactericidal activities in various strains of Gram-negative and Gram-positive bacteria. Interestingly, NPs showed antimicrobial activity through multiple mechanisms, such as cell membrane damage, protein leakage, and reactive oxygen species generation, and were more effective against gram-positive bacteria. Furthermore, in vitro scratch assay revealed that ZnFeO NPs improved cell migration and proliferation of cells, with noticeable shrinkage of the artificial wound model.

Conclusions: This study indicated that ZnFeO NPs have the potential to be used as a future antimicrobial and wound healing drug.
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http://dx.doi.org/10.1186/s12951-021-00776-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7866648PMC
February 2021

Pharmaceutical and Biomedical Applications of Green Synthesized Metal and Metal Oxide Nanoparticles.

Curr Pharm Des 2020 ;26(45):5844-5865

Department of Biomedical Sciences, Pak-Austria Fachhochschule: Institute of Applied Sciences and Technology, Mang, Khanpur Road, Haripur, Pakistan.

Background: Due to the rapid growth in life threatening diseases such as cancer, diabetes, chronic wound and HIV/AIDS along with rise of side effects of the current treatments, world is now focusing to utilize new treatment options. Currently, the development of green nanotechnology field seems as a potential alternate for diseases diagnosis and treatment by preparation of various sizes and shapes of nanomaterials.

Objective: This review is to present the explored biological sources in synthesis of nanomaterials particularly metal and metal oxides nanoparticles and critical review of the applications of biosynthesized nanoparticles in pharmaceutical and biomedical fields.

Methods: In this review, the various biological sources including bacteria, fungi, algae and plants used in synthesis of nanomaterials and mechanism involved in preparation are elaborated. In addition, biosynthesized nanomaterials applied as drug delivery system for anticancer, antibiotic, antidiabetic agent and functioned as potential diagnostic, antimicrobial, anticancer and wound healing candidates are comprehensively reviewed.

Results: The synthesized metal and metal oxides from green protocol proved to have advantages such as being biocompatible, effective and cheap. Furthermore, the green synthesized metal and metal oxide nanoparticles showed to possess prominent physical, chemical and biological properties that can be efficiently utilized for pharmaceutical and biomedical applications.

Conclusion: The information gathered in this review will provide a baseline for exploring more potential usage of green synthesized metal and metal oxide nanomaterials for various other applications. However, a concrete understanding of the safety of these nanomaterials is still needed to minimize the potential side effects.
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http://dx.doi.org/10.2174/1381612826666201126144805DOI Listing
April 2021

Corrigendum to "What Do the Hospital Pharmacists Think about the Quality of Pharmaceutical Care Services in a Pakistani Province? A Mixed Methodology Study".

Biomed Res Int 2018 27;2018:8705128. Epub 2018 Feb 27.

Department of Environmental Sciences, COMSATS Institute of Information Technology, Abbottabad 22060, Pakistan.

[This corrects the article DOI: 10.1155/2015/756180.].
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http://dx.doi.org/10.1155/2018/8705128DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5848052PMC
February 2018

Corrigendum to "What Do the Hospital Pharmacists Think about the Quality of Pharmaceutical Care Services in a Pakistani Province? A Mixed Methodology Study".

Biomed Res Int 2018 27;2018:8705128. Epub 2018 Feb 27.

Department of Environmental Sciences, COMSATS Institute of Information Technology, Abbottabad 22060, Pakistan.

[This corrects the article DOI: 10.1155/2015/756180.].
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http://dx.doi.org/10.1155/2018/8705128DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5848052PMC
February 2018

Randomized controlled trials covering pharmaceutical care and medicines management: A systematic review of literature.

Res Social Adm Pharm 2018 06 19;14(6):521-539. Epub 2017 Jun 19.

School of Pharmacy, University of Auckland, Private Mail Bag 92019, Auckland, New Zealand.

Objective: To review the effects of pharmaceutical care on hospitalizations, mortality and clinical outcomes in patients.

Methods: Systematic searches were conducted in MEDLINE, EMBASE and International Pharmaceutical Abstracts (IPA) databases to identify studies that were published between 2004 and January 2017. Studies included in this review were randomized controlled trials (RCTs) that spanned across both community and hospital settings. Using strict inclusion/exclusion criteria studies were included if they reported level 1 or 2 outcomes in the hierarchy of outcome measure i.e. clinical and surrogate outcomes (e.g. blood pressure (BP) control, blood glucose level, cholesterol BMI). Each study was assessed for quality using the Jadad scoring system.

Results: Fifty-four RCTs were included in the present review. Forty-six of these studies ranked high quality according to the Jadad scoring system. Studies were categorized into their general condition groups. Interventions in patients with diabetes, depression, respiratory disorders, cardiovascular disorders, epilepsy, osteoporosis, and interventions in older adults were identified. In the majority of studies pharmaceutical care was found to lead to significant improvements in clinical outcomes and/or hospitalizations when compared to the non-intervention group. Some conditions had a large number of RCTs, for example for cardiovascular conditions and in diabetes. Statistically significant improvements were seen in the majority of the studies included for both of these conditions, with studies indicating positive clinical outcomes and/or hospitalizations rates. Within the cardiovascular condition, a subset of studies, focusing on cardiac heart failure and coronary heart disease, had more mixed results. In other conditions the number of RCTs conducted was small and the evidence did not show improvements after pharmaceutical care, i.e. in depression, osteoporosis, and epilepsy. The majority of interventions were face to face interactions with patients, whilst a smaller number were conducted via the telephone and one via a web-based system. Patient education was a key component of most interventions, either verbal and/or written. Longitudinal data, post intervention cessation, was not collected in the majority of cases.

Conclusions: RCTs conducted to evaluate pharmaceutical care appear to be effective in improving patient short-term outcomes for a number of conditions including diabetes and cardiovascular conditions, however, other conditions such as depression are less well researched. Future research should attempt to evaluate the conditions where there is a lack of data, whether the positive effects of pharmaceutical care persist in patient populations after the interventions cease and what the long-term clinical outcomes would be of continued pharmaceutical care.
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http://dx.doi.org/10.1016/j.sapharm.2017.06.008DOI Listing
June 2018

EVOLVING SCENARIO OF PHARMACEUTICAL CARE IN PAKISTAN AND OTHER COUNTRIES: HEALTH IMPACT ASSESSMENT IN PUBLIC HEALTH PRACTICE.

Acta Pol Pharm 2016 Sep;73(5):1101-1109

Worldwide, pharmaceutical care has been recognized as the primary mission of pharmacy. According to the philosophy of pharmaceutical care, pharmacist is not only responsible to dispense the medicines but also responsible to improve the patient's quality of life. Pharmaceutical care practice is required to be introduced in the developing countries to decrease drug related mortality and morbidity. This paper aimed to highlight the quality of pharmaceutical care practice in the developing countries, predominantly in Pakistan. The paper highlights the health status and current scenario and barriers to pharmaceutical care practice in Pakistan. Pharmacists in Pakistan are not much involved in the provision of pharmaceutical care services due to a number of barriers that include insufficient number of pharmacists, lack of proper time, inadequate skills and training, lack of fmancial support and limited recognition of pharmacists in the public. A majority of community pharmacies are running without a pharmacist under the supervision of unprofessional personnel.
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September 2016

What do the hospital pharmacists think about the quality of pharmaceutical care services in a Pakistani province? A mixed methodology study.

Biomed Res Int 2015 8;2015:756180. Epub 2015 Jan 8.

Department of Environmental Sciences, COMSATS Institute of Information Technology, Abbottabad 22060, Pakistan.

The objective of this study was to evaluate the perception of hospital pharmacists regarding quality of pharmaceutical care services in Khyber Pakhtunkhwa (KPK) Province, Pakistan, through qualitative as well as quantitative approach. For qualitative study, snow ball sampling technique was used. In quantitative part, a cross-sectional study was conducted in 112 hospital pharmacists (out of 128 accessed ones) from both private and public hospitals in six major divisions (divisions are the third tier of government in Pakistan, between the provinces and districts) of KPK. The qualitative study yielded five major themes during thematic analysis: (a) patients reporting, (b) lack of patient counseling, (c) lack of participation in health awareness programs, (d) pharmacists reducing the prescribing errors, and (e) insufficient number of pharmacists. A great proportion (67.9%) of the pharmacists was unsatisfied with their participation in health awareness programs. Findings of both phases revealed that hospital pharmacists in Pakistan are not actively participating in the provision of pharmaceutical care services. They are facing various hurdles for their active participation in patient care; major obstacles include the unavailability of sufficient number of pharmacists, lack of appropriate time for patient counseling, and poor relationship between pharmacists and other health care providers.
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http://dx.doi.org/10.1155/2015/756180DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4306404PMC
September 2015

Iron deficiency anaemia in reproductive age women attending obstetrics and gynecology outpatient of university health centre in Al-Ahsa, Saudi Arabia.

Afr J Tradit Complement Altern Med 2014 28;11(2):339-42. Epub 2014 Jan 28.

Department of Pharmaceutical Sciences.

Background: Iron deficiency is the most common nutritional disorder in the world. The aim of this questionnaire based survey study was to determine the prevalence of iron deficiency anemia in reproductive age women, and their relation to variables such as age, marital status, education with those attending obstetrics and gynecology outpatient of King Faisal University Health Centre in Al-Ahsa in eastern region of Kingdom of Saudi Arabia.

Materials And Methods: This study was conducted for the period of 6 month staring from September 2012 to February 2013. The questionnaire had three sections on personal information: their educational indicators, gynecological clinical history, and hematological indices.

Results: The average age was 25.97±7.17 years. According to the gynecological clinical history of the respondents, 15 (48.4%) respondents were pregnant while 16 (51.6%) were not pregnant. There was significant effect of pregnancy status on Hb level. Majority of the anemic respondents 15/17 were married. Moreover 14/17 anemic women were experiencing severe menstrual bleeding, 11/17 respondents were pregnant. 54.8% of respondents were hemoglobin deficient while 77.4% were found to have low Hct. In 87.1 % of the respondents, transferrin saturation was found to be abnormal.

Conclusion: In this study iron deficiency anemia is quite prevalent in the university community especially among pregnant women. The fetus's and newborn infant's iron status depends on the iron status of the pregnant woman and therefore, iron deficiency in the mother-to-be means that growing fetus probably will be iron deficient as well. Thus iron deficiency anemia during pregnancy in well-educated set up needs more attention by the concerned authorities.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4202643PMC
http://dx.doi.org/10.4314/ajtcam.v11i2.19DOI Listing
July 2015

Pharmacokinetic study of hydroxypropylmethylcellulose microparticles loaded with cimetidine.

Adv Clin Exp Med 2013 Jan-Feb;22(1):41-5

Faculty of Pharmacy & Alternative Medicine, the Islamia University of Bahawalpur, Punjab, Pakistan.

Objectives: The objective of this study was to assess the pharmacokinetic behavior of floating hydroxypropylmethylcellulose microparticles loaded with cimetidine (FMC) prepared using the non-solvent addition coacervation technique.

Material And Methods: Based on the physico-chemical characteristics of three formulations (FMC1, FMC2 and FMC3), FMC2 having a 1:3 ratio of cimetidine:HPMC was found optimum. For in vivo analysis, a new HPLC analytical method was developed and validated. The optimized formulations were subjected to in vivo studies to calculate the various pharmacokinetic parameters for developed optimized microparticulate formulation FMC3. The developed floating microparticles of cimetidine were further evaluated by in vivo experimentation.

Results: The bioavailability parameters were found as: Cmax 1508.79 ± 37.95 ng/ml, Tmax 3.67 ± 0.17 h and AUC 14366.19 ± 377.64 ng h /mL.

Conclusions: For prolonged drug release in the stomach, developed floating microparticles of cimetidine (FMC3) may be used, thereby improving the bioavailability and patient compliance.
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June 2013

In vitro-in vivo correlation study on nimesulide loaded hydroxypropylmethylcellulose microparticles.

Yao Xue Xue Bao 2010 Jun;45(6):772-7

Faculty of Pharmacy & Alternative Medicine, Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan.

This study involves mathematical simulation model such as in vitro-in vivo correlation (IVIVC) development for various extended release formulations of nimesulide loaded hydroxypropylmethylcellulose (HPMC) microparticles (M1, M2 and M3 containing 1, 2, and 3 g HPMC, respectively and 1 g drug in each) having variable release characteristics. In vitro dissolution data of these formulations were correlated to their relevant in vivo absorption profiles followed by predictability worth analysis of these Level A IVIVC. Nimaran was used as control formulation to validate developed formulations and their respective models. The regression coefficients of IVIVC plots for M1, M2, M3 and Nimaran were 0.834 9, 0.831 2, 0.927 2 and 0.898 1, respectively. The internal prediction error for all formulations was within limits, i.e., < 10%. A good IVIVC was found for controlled release nimesulide loaded HPMC floating M3 microparticles. In other words, this mathematical simulation model is best fit for biowaiver studies which involves study parameters as those adopted for M3 because the value of its IVIVC regression coefficient is the closest to 1 as compared to M1 and M2.
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June 2010

Formulation of two-drug controlled release non-biodegradable microparticles for potential treatment of muscles pain and spasm and their simultaneous spectrophotometeric estimation.

Acta Pol Pharm 2010 May-Jun;67(3):299-306

Department of Pharmacy, Faculty of Pharmacy and Alternative Medicines, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan.

The objective of this study was to formulate stable and controlled release microparticles for simultaneous delivery and UV spectrophotometric detection in combined dosage of an non-steroidal anti-inflammatory drug (NSAID) (nimesulide, NMS) and a spasmolytic agent (tizanidine, TZN) to maintain plasma concentration that may increase patients compliance, improved therapeutic efficacy, The aim was also to reduce severity of upper GI side effects of NMS because of alteration in delivery pattern via slow release of drug from microparticles and to increase the benefits of spasticity and disability for spastic patients by administering TZN in a modified release formulation as these two drugs are often prescribed in combination for the management of pain associated with muscles spasm. Ethyl cellulose was used as a retardant polymer. Drug-polymer and drug-drug compatibility study were conducted by different analytical tests. Microparticles were prepared by coacervation thermal change method. The prepared microparticles were characterized for their micromeritics and drug loading. The prepared microparticles were light yellow, free flowing and spherical in shape. The drug-loaded microparticles showed 87% and 91% entrapment efficiency of NMS and TZN, respectively, and release was extended up to 10 h. The infrared spectra, differential scanning calorimetry thermograms and XRD spectra showed the stable character of both the drugs in the drug-loaded microparticles. The in vitro release study of microparticles was performed in phosphate buffer pH 6.8. Linearity was observed in the concentration range of 5.0-30.0 microg/mL of NMS and 0.5-3.0 microg/mL of TZN. The microparticles have a potential for the prolongation and simultaneous delivery of the NIM and TIZ. The proposed UV method for simultaneous detection can be used for routine analysis of combined dosage form.
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June 2010
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