Publications by authors named "Rossana Giancaspro"

7 Publications

  • Page 1 of 1

Chronic rhinosinusitis with nasal polyposis (CRSwNP): the correlation between expression of Galectin-10 and Clinical-Cytological Grading (CCG).

Am J Rhinol Allergy 2021 Oct 14:19458924211049867. Epub 2021 Oct 14.

Unit of Clinical Pathology and Center for Molecular Medicine, 507873Department of Medical and Surgical Sciences, 18972University of Foggia, Foggia, Italy.

Background: Chronic rhinosinusitis with nasal polyps (CRSwNP) is typically characterized by Type 2 inflammation. Several biomarkers of eosinophilic inflammation, including Galectin-10, also known as Charcot-Leyden crystal protein (CLCP), have been identified to establish eosinophilic infiltration of polyps, a reliable predictor of recurrence. We aimed to evaluate the Galectin-10 expression in nasal polyps of patients with CRSwNP and to assess the correlation of Charcot-Leyden crystals expression to the severity of CRSwNP according to Clinical-Cytological Grading (CCG).

Methods: A double-label immunofluorescence was performed to evaluate the expression of Gal-10, CD15, Tryptase, and CD63 and their eventual co-localization on histological samples of 18 patients with CRSwNP. Double-positive Gal-10+CD15+ and Galectin-10+Tryptase+ inflammatory cells were counted by confocal microscopy.

Results: Galectin-10 was detectable in all examined tissues from CRSwNP patients, and its expression increased as low, medium and high CCG tissues were examined, respectively. Galectin-10 was extensively present in inflammatory cells, while limited Galectin-10 deposits were detected around mucosal epithelial cells.

Conclusion: We showed the strong correlation between CCG and Galectin-10 expression, mainly colocalized with infiltrating eosinophils and mast-cells, in patients affected by CRSwNP.
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http://dx.doi.org/10.1177/19458924211049867DOI Listing
October 2021

The Underestimated Role of Mast Cells in the Pathogenesis of Rhinopathies.

Int Arch Allergy Immunol 2021 Sep 22:1-7. Epub 2021 Sep 22.

Department of Basic Medical Sciences, Neurosciences and Sensory Organs, University of Bari Medical School, Bari, Italy.

Mast cells (MCs) are involved in several biological processes, such as defense against pathogens, immunomodulation, tissue repair after injury, and angiogenesis. MCs have been shown to change from protective immune cells to potent pro-inflammatory cells, influencing the progression of many pathological conditions, including autoimmune diseases and cancers. The role of MCs in the pathogenesis of rhinopathies has often been underestimated, since previous studies have focused their attention on eosinophils and neutrophils, while MCs were considered involved exclusively in allergic rhinitis. However, recent nasal cytology findings have shown the involvement of MCs in several rhinopathies, such as NARMA, NARESMA, and CRSwNP. These recent evidences highlight the crucial role that MCs play in orchestrating the inflammation of the nasal mucosa, through complex biological mechanisms, not yet fully understood. In this context, a better understanding of these mechanisms is fundamental for practicing Precision Medicine, which requires careful population selection and stratification into subgroups based on the phenotype/endotype of the patients, in order to guarantee the patient a tailored therapy. Based on this background, further studies are needed to understand the pathophysiological mechanisms involving MCs and, consequently, to develop targeted therapies aimed to obtain a selective inhibition of tissue remodeling and preventing MC-mediated immune suppression.
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http://dx.doi.org/10.1159/000518924DOI Listing
September 2021

Dysphagia in non-intubated patients affected by COVID-19 infection.

Eur Arch Otorhinolaryngol 2021 Sep 1. Epub 2021 Sep 1.

Department of Otolaryngology-Head and Neck Surgery, University of Foggia, Via Luigi Pinto 1, 71122, Foggia, Italy.

Purpose: Patients affected by COVID-19 are assumed to be at high risk of developing swallowing disorders. However, to our best knowledge, data on the characteristics and incidence of dysphagia associated with COVID-19 are lacking, especially in non-intubated patients. Therefore, we investigated the onset of swallowing disorders in patients with laboratory-confirmed COVID-19 infection who have not been treated with invasive ventilation, in order to evaluate how the virus affected swallowing function regardless of orotracheal intubation.

Methods: We evaluated 41 patients admitted to the COVID department of our Hospital when they had already passed the acute phase of the disease and were therefore asymptomatic but still positive for SARS-CoV-2 RNA by RT-PCR. We examined patients' clinical history and performed the Volume-Viscosity Swallow Test (VVST). Each patient also answered the Swallowing Disturbance Questionnaire (SDQ). After 6 months, we performed a follow-up in patients with swallowing disorders.

Results: Eight of 41 patients (20%) presented with dysphagia symptoms during hospitalization and 2 of them (25%) still presented a SDQ high score and swallowing disorders with liquid consistency after 6 months.

Conclusion: Non-intubated patients can experience various grades of swallowing impairment that probably directly related to pulmonary respiratory function alterations and viral direct neuronal lesive activity. Although these symptoms show natural tendency to spontaneous resolution, their impact on a general physical impaired situation should not be underestimated, since it can adversely affect patients' recovery from COVID-19 worsening health outcomes.
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http://dx.doi.org/10.1007/s00405-021-07062-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8408570PMC
September 2021

Nasal cytology in allergic rhinitis: Rare observation of pollen degranulation.

Int Forum Allergy Rhinol 2021 Dec 29;11(12):1710-1711. Epub 2021 Jun 29.

Department of Otolaryngology, University Hospital of Foggia, Foggia, Italy.

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http://dx.doi.org/10.1002/alr.22860DOI Listing
December 2021

Role of fern test in rhinosinusitis.

Int Forum Allergy Rhinol 2021 Dec 27;11(12):1712-1713. Epub 2021 Jun 27.

Department of Otolaryngology, University Hospital of Foggia, Foggia, Italy.

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http://dx.doi.org/10.1002/alr.22857DOI Listing
December 2021

Surgical mask and N95 in healthcare workers of Covid-19 departments: clinical and social aspects.

Acta Biomed 2020 11 10;91(4):e2020171. Epub 2020 Nov 10.

University of Foggia.

Introdution. In order to prevent or slow down the transmission of COVID-19, various public health measures have been introduced, including social distancing, environmental disinfection and the use of personal protective equipment (PPE). In this perspective, the clinical practice of healthcare professionals has changed dramatically. As a matter of fact, the use of surgical masks and N95 has significantly worsened the job performance of workers who deal directly with COVID-19 disease.

Methods: The study included 116 health workers employed in the pulmonology, intensive care and infectious diseases departments of Bari and Foggia Hospital, directly involved in the healthcare of patients affected by COVID-19. Between May 1, 2020 and May 31, 2020, each participant completed an online questionnaire aimed to investigate the impact of the COVID-19 pandemic on workers' lifestyle changes and job performances. We compared the results based on the type of mask used by each participant (surgical mask vs N95).

Results: Although disturbances related to the use of the mask arose earlier in subjects who wore the N95 (p = 0.0094), healthcare workers that wore surgical masks reported a statistically higher average score for a greater number of disorders.

Conclusions: This is the first study that compares the effects of the two most used PPE on the quality of life of health workers and which highlights the greater discomfort caused by surgical masks. This result brings to light a serious social problem, being surgical masks widely used in everyday life by ordinary people and non-healthcare workers.
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http://dx.doi.org/10.23750/abm.v91i4.10660DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7927551PMC
November 2020
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