Publications by authors named "Ronghua Zhu"

22 Publications

  • Page 1 of 1

Clone Selection Artificial Intelligence Algorithm-Based Positron Emission Tomography-Computed Tomography Image Information Data Analysis for the Qualitative Diagnosis of Serous Cavity Effusion in Patients with Malignant Tumors.

J Healthc Eng 2021 18;2021:4272411. Epub 2021 Dec 18.

Department of Nuclear Medicine, Huai'an First People's Hospital, 6 Beijing West Road, Huaiyin District, Huai'an 223300, Jiangsu, China.

This study aimed to investigate the application of positron emission tomography- (PET-) computed tomography (CT) image information data combined with serous cavity effusion based on clone selection artificial intelligence algorithm in the diagnosis of patients with malignant tumors. A total of 97 patients with PET-CT scanning and empirically confirmed as serous cavity effusion were retrospectively analyzed in this study. The clone selection artificial intelligence algorithm was applied to register the PET-CT images, and the patients were rolled into a benign effusion group and a malignant effusion group according to the benign and malignant conditions of the serous cavity effusion. Besides, the causes of patients from the two groups were analyzed, and there was a comparison of their physiological conditions. Subsequently, CT values of different KeV, lipid/water, water/iodine, and water/calcium concentrations were measured, and the differences of the above quantitative parameters between benign and malignant serous cavity effusion were compared, as well as the registration results of the clone algorithm. The results showed that the registration time and misalignment times of clonal selection algorithm (13.88, 0) were lower than those of genetic algorithm (18.72, 8). There were marked differences in CT values of 40-60 keV and 130-140 keV between the two groups. The concentrations of lipid/water, water/iodine, and water/calcium in basal substances of the malignant effusion group were obviously higher than the concentrations of the benign effusion group ( < 0.05). Benign and malignant effusions presented different manifestations in PET-CT, which was conducive to the further diagnosis of malignant tumors. Based on clone selection artificial intelligence algorithm, PET-CT could provide a new multiparameter method for the identification of benign and malignant serous cavity effusions and benign and malignant tumors.
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http://dx.doi.org/10.1155/2021/4272411DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8710161PMC
January 2022

Effects of Dietary Mannan Oligosaccharides on Non-Specific Immunity, Intestinal Health, and Antibiotic Resistance Genes in Pacific White Shrimp .

Front Immunol 2021 24;12:772570. Epub 2021 Nov 24.

The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture), The Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao, China.

This study was conducted to comprehensively investigate the beneficial effects of a mannan oligosaccharide product (hereinafter called MOS) on and optimum level of MOS. Five isonitrogenous and isolipid diets were formulated by adding 0%, 0.02%, 0.04%, 0.08%, and 0.16% MOS in the basal diet. Each diet was randomly fed to one group with four replicates of shrimp in an 8-week feeding trial. The results showed that dietary MOS improved the growth performance and the ability of digestion of shrimp. Dietary MOS significantly increased the activity of total superoxide dismutase, catalase, and glutathione peroxidase and decreased the content of malondialdehyde in plasma of shrimp. Dietary MOS significantly increased the activity of alkaline phosphatase and lysozyme in plasma and the hemocyte counts. Dietary MOS significantly upregulated the expression of Toll, lysozyme, anti-lipopolysaccharide factor, Crustin, and heat shock protein 70 in the hepatopancreas. And dietary MOS significantly upregulated the expression of intestinal mucin-2, mucin-5B, and mucin-19, while it decreased the expression of intestinal mucin-1 and macrophage migration inhibitory factor. Dietary MOS improved the bacterial diversity; increased the abundance of , , , and ; and decreased the abundance of in the intestine. Shrimp fed MOS diets showed lower mortality after being challenged by . Notably, this study found a decrease in antibiotic resistance genes and mobile genetic elements after MOS supplementation for the first time. The present results showed that diet with MOS supplementation enhanced the organismal antioxidant capacity and immunity, improved intestinal immunity, optimized intestinal microecology, mitigated the degree of antibiotic resistance, and increased the resistance to in , especially when supplemented at 0.08% and 0.16%.
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http://dx.doi.org/10.3389/fimmu.2021.772570DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8652215PMC
November 2021

A Numerical Study on 3D Printed Cementitious Composites Mixes Subjected to Axial Compression.

Materials (Basel) 2021 Nov 15;14(22). Epub 2021 Nov 15.

Institute of Marine Structures and Naval Architectures, Ocean College, Zhejiang University, Zhoushan 316021, China.

Aptly enabled by recent developments in additive manufacturing technology, the concept of functionally grading some cementitious composites to improve structural compression forms is warranted. In this work, existing concrete models available in Abaqus Finite Element (FE) packages are utilized to simulate the performance of some cementitious composites numerically and apply them to functional grading using the multi-layer approach. If yielding good agreement with the experimental results, two-layer and three-layer models case combinations are developed to study the role of layer position and volume. The optimal and sub-optimal performance of the multi-layer concrete configurations based on compressive strength and sustained strains are assessed. The results of the models suggest that layer volume and position influence the performance of multi-layer concrete. It is observed that when there exists a substantial difference in material strengths between the concrete mixes that make up the various layers of a functionally graded structure, the influence of position and of material volume are significant in a two-layer configuration. In contrast, in a three-layer configuration, layer position is of minimal effect, and volume has a significant effect only if two of the three layers are made from the same material. Thus, a multilayered design approach to compression structures can significantly improve strength and strain performance. Finally, application scenarios on some structural compression forms are shown, and their future trajectory is discussed.
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http://dx.doi.org/10.3390/ma14226882DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8623701PMC
November 2021

Single Dose of SHR-1222, a Sclerostin Monoclonal Antibody, in Healthy Men and Postmenopausal Women With Low Bone Mass: A Randomized, Double-Blind, Placebo-Controlled, Dose-Escalation, Phase I Study.

Front Pharmacol 2021 20;12:770073. Epub 2021 Oct 20.

National Clinical Research Center for Metabolic Diseases, Key Laboratory of Diabetes Immunology, Ministry of Education, and Department of Metabolism and Endocrinology, The Second Xiangya Hospital of Central South University, Changsha, China.

SHR-1222 is a humanized monoclonal antibody targeting sclerostin and has the potential to promote bone formation and reduce bone resorption. This study was aimed to assess the safety, tolerability, pharmacokinetics, pharmacodynamics, and immunogenicity of SHR-1222 in healthy men and postmenopausal women with low bone mass (BMD). It was a randomized, double-blind, placebo-controlled, dose-escalation, phase I study. Subjects received SHR-1222 at 50, 100, 200, 300, and 400 mg sequentially or matching placebo subcutaneously. Totally, 50 subjects with low BMD were enrolled and randomly assigned; 10 received placebo and 40 received SHR-1222 (50 mg, n = 4; 100, 200, 300, or 400 mg, n = 9). The most common adverse events that occurred at least 10% higher in subjects with SHR-1222 treatment than those with placebo were decreased blood calcium, blood urine present, increased blood cholesterol, electrocardiogram T wave abnormal, urinary tract infection, increased blood pressure diastolic, and positive bacterial test. All the above adverse events were mild in severity and well resolved except one of increased blood cholesterol in a subject lost to follow-up. The serum SHR-1222 concentration increased in a dose-dependent manner. Administration of SHR-1222 upregulated the bone-formation markers N-terminal propeptide of type 1 procollagen, osteocalcin, and bone-specific alkaline phosphatase, while downregulated the bone-resorption marker β-C-telopeptide. The BMD at the lumbar spine notably rose after a single dose of SHR-1222. The largest increase occurred in the 400 mg cohort (3.8, 6.7, and 6.1% on day 29, 57, and 85, respectively; compared with 1.4, 0.8, and 1.0% in the placebo group). Although 10.0% of subjects receiving SHR-1222 tested positive for anti-SHR-1222 antibodies, no obvious effects of antibody formation were found on pharmacokinetics. Overall, SHR-1222 was well tolerated at doses from 50 to 400 mg and is a promising new remedy for osteoporosis. http://www.clinicaltrials.gov, NCT03870100.
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http://dx.doi.org/10.3389/fphar.2021.770073DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8564351PMC
October 2021

Chlorogenic acid ameliorates -induced pneumonia in immunosuppressed mice inhibiting the activation of NLRP3 inflammasomes.

Food Funct 2021 Oct 4;12(19):9466-9475. Epub 2021 Oct 4.

College of Medical Science, China Three Gorges University, Yichang, Hubei, 443000, China.

Chlorogenic acid (CGA) possesses a wide variety of bioactive properties, such as antioxidation, anti-inflammation and anti-bacteria. This study was aimed at exploring the effects of CGA of anti-inflammation and anti-bacteria on mouse pneumonia prepared by immunosuppressed mice infected with () and the cellular inflammasomes through lipopolysaccharide (LPS) and adenosine triphosphate (ATP)-induced RAW 264.7 murine macrophages . Mice received CGA treatment (30 and 90 mg kg) for 8 consecutive days and on the fourth day immunosuppression in mice was induced by cyclophosphamide (40 mg kg) for 5 days before inoculation of . Immunosuppressed mice infected with developed severe pneumonia, with marked interstitial vascular congestion, widened alveolar intervals, infiltration of monocytes, lymphocytes and macrophages as well as the damage of epithelial architecture, with growing mortality and count forming unit (CFU). CGA treatment significantly decreased the ratio of lung/body weight, reduced the severity of pneumonia induced by , decreased the lung injury, inflammatory cell infiltration scores and CD68 protein expression, inhibited the expression of interleukin (IL)-6, IL-8, tumor necrosis factor (TNF)-α, and elevated the expression of IL-10. Meanwhile, we investigated the mechanism of CGA to counter -induced pneumonia and found that CGA remarkably repressed the activation of nucleotide-binding domain like receptor protein 3 (NLRP3) inflammasome. Altogether, our results indicate that the dietary intake of CGA or its rich foods ameliorates -induced pneumonia by inhibiting the activation of NLRP3 inflammasomes.
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http://dx.doi.org/10.1039/d0fo03185bDOI Listing
October 2021

[Simultaneous determination of 37 mycotoxins in grain and animal feed by impurity adsorption purification coupled with ultra-performance liquid chromatography-tandem mass spectrometry].

Se Pu 2020 Jul;38(7):817-825

Alltech Biological Products(China)Co., Ltd, Beijing 100600, China.

A rapid method based on ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed for the simultaneous determination of 37 mycotoxins having various physicochemical properties in grain and animal feed samples. The 37 analytes were extracted from ground samples with acetonitrile-water-formic acid (84:15.9:0.1, v/v/v) by 20 min vibration, and purified using a commercial MLJ-1 pass-through solid-phase extraction (SPE) clean-up cartridge. The analytes were then separated on a reversed-phase BEH RP18 column by a gradient elution program with 0.1 mmol/L ammonium acetate (containing 0.1% (v/v) formic acid) aqueous solution and 0.1% (v/v) formic acid methanol solution as mobile phases. The separated analytes were detected by MS/MS in the multiple reaction monitoring (MRM) mode via ESI+/- ionization. The results showed that the purification was completed in 1 min and that the 37 analytes could be separated on the chromatographic column in 15 min. The 37 mycotoxins showed a linear relationship within their respective linear ranges, and the correlation coefficients of the matrix-matched calibration curves were greater than 0.98. The average recoveries at four spiked levels (limit of quantification (LOQ), LOQ×5, LOQ×10, LOQ×25) for all the targets except fumonisins ranged from 80% to 120%, with the relative standard deviations (RSDs) lower than 20% (=6). The limits of quantification (LOQs) for all the analytes were between 2 and 40 μg/kg. The proposed method is simple, fast, and accurate, thus being suitable for detecting multiple mycotoxins in grain and animal feed samples.
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http://dx.doi.org/10.3724/SP.J.1123.2019.12013DOI Listing
July 2020

Evaluation of bioequivalence between generic and brand-name clozapine in Chinese schizophrenic patients: A randomized, two-period, crossover study.

Int J Clin Pharmacol Ther 2021 Aug;59(8):578-584

Objective: Clozapine is the most effective therapy for schizophrenia. This study compared the bioequivalence of a generic formulation of clozapine (ChangZhou Pharmaceutical Factory Co. Ltd. Jiangsu, China) to the brand name formulation (Clozaril, HLS Therapeutics, Inc., Philadelphia, PA, USA) after multiple doses in Chinese schizophrenic patients.

Materials And Methods: This was a randomized, open-label, multiple-dose, 2-way crossover study in which patients with schizophrenia received the generic clozapine or Clozaril 100 mg twice daily for 10 days before crossing over to the alternate formulation for the next 10 days. Blood samples were collected at regular intervals during each treatment period, and plasma concentration of clozapine was determined by high-performance liquid chromatography.

Results: 26 patients were enrolled, of whom 24 completed the study and were included in the steady-state analyses. The mean AUC was 6,003.29 h×ng/mL for generic clozapine and 6,347.53 h×ng/mL for Clozaril. The mean C was 698.52 ng/mL for generic clozapine and 739.75 ng/mL for Clozaril. The ratio of the adjusted geometric means and its 90% CI of the ratios for AUC was 96.24% (89.60 - 103.36%), and for C was 95.90% (88.91 - 103.44%). A total of 66 adverse events were reported by 22 (84.62%) subjects. Among them, 34 occurred in 17 (65.38%) patients during dosing of generic clozapine, and 32 occurred in 16 (61.54%) patients during dosing of brand-name clozapine.

Conclusion: The result demonstrated that the generic clozapine was bioequivalent to brand-name clozapine (Clozaril). This would provide physicians with reassurance that patients who receive the studied generic clozapine will achieve similar plasma drug concentrations to those of brand-name clozapine (Clozaril).
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http://dx.doi.org/10.5414/CP203864DOI Listing
August 2021

Self-Triggered Thermomechanical Metamaterials with Asymmetric Structures for Programmable Response under Thermal Excitations.

Materials (Basel) 2021 Apr 23;14(9). Epub 2021 Apr 23.

Chongqing Key Laboratory of Nonlinear Circuits and Intelligent Information Processing, Southwest University, Chongqing 400715, China.

In this study, we propose self-triggered thermomechanical metamaterials (ST-MM) by applying thermomechanical materials in mechanical metamaterials designed with asymmetric structures (i.e., microstructural hexagons and chiral legs). The thermomechanical metamaterials are observed with programmable mechanical response under thermal excitations, which are used in mechanical metamaterials to obtain chiral tubes with negative Poisson's ratio and microgrippers with temperature-induced grabbing response. Theoretical and numerical models are developed to analyze the thermomechanical response of the ST-MM from the material and structural perspectives. Finally, we envision advanced applications of the ST-MM as chiral stents and thermoresponsive microgrippers with maximum grabbing force of approximately 101.7 N. The emerging ST-MM provide a promising direction for the design and perception of smart mechanical metamaterials.
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http://dx.doi.org/10.3390/ma14092177DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8123077PMC
April 2021

Evaluation of the mitigation efficacy of a yeast cell wall extract toward deoxynivalenol contaminated diet fed to turbot (Scophthalmus maximus).

Ecotoxicol Environ Saf 2021 Apr 13;216:112221. Epub 2021 Apr 13.

The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture), The Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao 266003, China.

Deoxynivalenol (DON) is one of the most common mycotoxins in animal feed worldwide and causes significant threats to the animal health. Increased use of plant ingredients in aquaculture feeds increased the risk of mycotoxin contamination. To evaluate the effects of dietary deoxynivalenol (DON) on growth performance, immune response and intestinal health of turbot and the mitigation efficacy of yeast cell wall extract (YCWE) toward DON, nine isonitrogenous and isolipidic diets were formulated: Diet 1 (control): No DON added; Diets 2-5 or Diets 6-9: 0.5 or 3.0 mg added DON/kg diet + 0%, 0.1%, 0.2%, or 0.4% YCWE, respectively. Results showed that Diet 6 (3 mg/kg DON, 0% YCWE) significantly decreased weight gain, specific growth rate and feed efficiency ratio of fish and reduced immunoglobulin M and complement 4 concentrations in serum. Fish fed Diet 6 presented morphological alterations, lower activity of superoxide dismutase, catalase and total antioxidant capacity but higher malondialdehyde content, lower claudin-4 and occludin expression but higher interleukin-1β expression in intestine. Besides, Diet 6 decreased the abundance of potential helpful bacteria but increased the abundance of potential pathogens in intestine. While, dietary YCWE, especially Diet 8 (3 mg/kg DON, 0.2% YCWE) and 9 (3 mg/kg DON, 0.4% YCWE), markedly improved growth performance and immune response and enhanced the intestinal health of turbot. In conclusion, dietary YCWE could mitigate the toxic effects induced by DON in turbot, and could be used as an effective strategy to control DON contamination in fish feed.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112221DOI Listing
April 2021

The Assessment of Diet Contaminated with Aflatoxin B in Juvenile Turbot () and the Evaluation of the Efficacy of Mitigation of a Yeast Cell Wall Extract.

Toxins (Basel) 2020 09 15;12(9). Epub 2020 Sep 15.

The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture), the Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao 266003, China.

This study aimed to investigate the effects of dietary AFB on growth performance, health, intestinal microbiota communities and AFB tissue residues of turbot and evaluate the mitigation efficacy of yeast cell wall extract, Mycosorb (YCWE) toward AFB contaminated dietary treatments. Nine experimental diets were formulated: Diet 1 (control): AFB free; Diets 2-5 or Diets 6-9: 20 μg AFB/kg diet or 500 μg AFB/kg diet + 0%, 0.1%, 0.2%, or 0.4% YCWE, respectively). The results showed that Diet 6 significantly decreased the concentrations of TP, GLB, C3, C4, T-CHO, TG but increased the activities of AST, ALT in serum, decreased the expressions of CAT, SOD, GPx, CYP1A but increased the expressions of CYP3A, GST-, p53 in liver. Diet 6 increased the AFB residues in serum and muscle, altered the intestinal microbiota composition, decreased the bacterial community diversity and the abundance of some potential probiotics. However, Diet 8 and Diet 9 restored the immune response, relieved adverse effects in liver, lowered the AFB residues in turbot tissues, promoted intestinal microbiota diversity and lowered the abundance of potentially pathogens. In conclusion, YCWE supplementation decreased the health effects of AFB on turbot, restoring biomarkers closer to the mycotoxin-free control diet.
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http://dx.doi.org/10.3390/toxins12090597DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7551837PMC
September 2020

WITHDRAWN: Dynamic Observation of Postoperative Infection and Neuron-specific Enolase Levels in Serum in Patients with Non-small Cell Lung Cancer According to Positron Emission Tomography-Computed Tomography.

Neurosci Lett 2020 Jul 2:135226. Epub 2020 Jul 2.

Department of Radiotherapy, Huai'an First People's Hospital, Huai'an, 223300, Jiangsu, China.

This article has been withdrawn at the request of the Editor-in-Chief. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at https://www.elsevier.com/about/our-business/policies/article-withdrawal.
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http://dx.doi.org/10.1016/j.neulet.2020.135226DOI Listing
July 2020

miR-21 modulates cisplatin resistance of gastric cancer cells by inhibiting autophagy via the PI3K/Akt/mTOR pathway.

Anticancer Drugs 2020 04;31(4):385-393

Department of General Surgery, Xinhua Hospital Chongming Branch, School of Medicine, Shanghai Jiaotong University, Shanghai, People's Republic of China.

Resistance to cisplatin (DDP) remains a major obstacle in the control of gastric cancer (GC) progression. A previous study revealed that microRNA-21 (miR-21) contributes to DDP resistance in GC cells via the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) pathway. The aim of the current study was to explore the mechanisms underlying the cytoprotective function of miR-21. In this study, DDP-resistant GC cells were obtained by continuous exposure of human gastric adenocarcinoma cells to increasing concentrations of DDP. Western blot analysis was used to evaluate activation of the PI3K/Akt/mechanistic target of rapamycin kinase (mTOR) pathway. The level of miR-21 was altered by transfection of miR-21 mimic and inhibitor. Autophagy was assessed by detecting autophagosome formation, Beclin-1 and LC3 expression. An Annexin V-propidium iodide assay was performed to estimate the survival and death of GC cells. GC cells became refractory to the growth inhibition and apoptosis induced by DDP treatment, activation of Akt and mTOR were increased in DDP-resistant GC cells. Inhibition of autophagy decreased the sensitivity of GC cells to DDP, and autophagy induction produced the opposite effect. DDP-resistant GC cells expressed higher levels of miR-21 compared with the parent cells. Transfection of GC cells with miR-21 mimics contributed to restored DDP resistance by suppressing autophagy, while miR-21 inhibitor sensitized DDP-resistant GC cells by promoting autophagy. In conclusion, the results demonstrated that miR-21 is associated with DDP resistance in GC cells by inhibiting autophagy via the PI3K/Akt/mTOR pathway, and autophagy inducers could be therapeutic targets for the effective treatment of DDP resistance in GC.
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http://dx.doi.org/10.1097/CAD.0000000000000886DOI Listing
April 2020

Novel Semi-Analytical Solutions for the Transient Behaviors of Functionally Graded Material Plates in the Thermal Environment.

Materials (Basel) 2019 Dec 6;12(24). Epub 2019 Dec 6.

Ocean College, Zhejiang University, Room 302, Ocean Engineering Building, Zhoushan Campus, Zhejiang University, Dinghai, Zhoushan 316021, China.

The primary objective of this article is to present a semi-analytical algorithm for the transient behaviors of Functionally Graded Materials plates (FGM plates) considering both the influence of in-plane displacements and the influence of temperature changes. Based on the classical plate theory considering the effect of in-plane displacements, the equilibrium equations of the motion system are derived by Hamilton's principle. Here, we propose a novel, accurate, and efficient semi-analytical method that incorporates the Fourier series expansion, the Laplace transforms, and its numerical inversion and the Differential Quadrature Method (DQM) to simulate the transient behaviors. This paper validates the proposed method by comparisons with semi-analytical natural frequency results and those from the literature. Expressly, the results of dynamic response also agree well with those generated by the Navier's method and Finite Element Method (FEM). A convergence study that utilizes the different numbers of sampling points shows that the process can converge quickly, and a few sampling points can achieve high accuracy. The effects of various boundary conditions at the ends, material graded index, and temperature change are further investigated. From the detailed parametric study, it is seen that the peak displacement increases as the edge degrees of freedom, the gradient index of the material, and temperature change increase.
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http://dx.doi.org/10.3390/ma12244084DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947207PMC
December 2019

Upregulation of lncRNA H19 promotes nasopharyngeal carcinoma proliferation and metastasis in let-7 dependent manner.

Artif Cells Nanomed Biotechnol 2019 Dec;47(1):3854-3861

The First Affiliated Hospital of Zhengzhou University , Zhengzhou , China.

The aim of this study is to analyse the expression status of long non-coding RNA (lncRNA) H19 in nasopharyngeal carcinoma and to unravel its oncogenic properties at molecular level. The abundance of H19, let-7a, b, g, i and HRAS was quantified by real-time PCR. Cell viability was measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. Cell proliferation was evaluated by the cell counting. Cell migration and cell invasion were determined using transwell chamber and scattering colony formation. Tumour progression was monitored in xenograft tumour model and tail vein injection was adopted for lung metastasis assessment. Luciferase reporter assay was employed to interrogate the potential regulatory action of let-7 genes on H19 expression. The endogenous HRAS protein was quantified by western blotting. H19 was aberrantly over-expression in nasopharyngeal carcinoma, which intimately associated with poorer prognosis. H19-deficency significantly inhibited cell viability and suppressed cell proliferation. Furthermore, both migrative and invasive capacity were compromised by H19 knockdown. H19-silencing remarkably delayed xenograft tumour progression and lung metastasis. Mechanistically, H19 competitively sponged let-7 genes and therefore up-regulated HRAS, which consequently contributed to its oncogenic activity in nasopharyngeal carcinomas. Our study uncovered the oncogenic properties of H19 in nasopharyngeal carcinoma and highlighted the H19-let-7-HRAS signalling axis underlying the incidence and metastasis of this disease.
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http://dx.doi.org/10.1080/21691401.2019.1669618DOI Listing
December 2019

Curcumin suppresses the progression of laryngeal squamous cell carcinoma through the upregulation of miR-145 and inhibition of the PI3K/Akt/mTOR pathway.

Onco Targets Ther 2018 19;11:3521-3531. Epub 2018 Jun 19.

Department of Otorhinolaryngology, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, People's Republic of China.

Background: Curcumin is a polyphenol extracted from the rhizomes of with extensive biological and pharmacological effects. The present study aimed to investigate the mechanisms of curcumin in laryngeal squamous cell carcinoma (LSCC).

Methods: Quantitative real-time reverse transcriptase-polymerase chain reaction was performed to detect the expressions of miR-145 in LSCC tissues and cells. The effects of miR-145 and curcumin on cell proliferation, apoptosis, cell cycle, migration and invasion were explored by MTT assay, flow cytometry analysis, Transwell migration and invasion assay, respectively. The effects of miR-145 combined with curcumin on the phosphoinositol 1,3 kinase (PI3K)/protein kinase B (Akt)/mammalian target of rapamycin (mTOR) pathway were detected by Western blot analysis.

Results: miR-145 was significantly downregulated in LSCC tissues and cells. Curcumin administration upregulated miR-145 expression in LSCC cells in a dose-dependent manner. miR-145 overexpression and curcumin treatment both markedly suppressed cell proliferation, migration and invasion and induced cell cycle arrest and apoptosis in LSCC cells. Moreover, curcumin treatment reversed the enhanced effects on cell viability, migration and invasion and the inhibitory effects on apoptosis conferred by anti-miR-145 in LSCC cells. Curcumin treatment dramatically aggravated miR-145-induced inhibition of the PI3K/Akt/mTOR pathway and reversed anti-miR-145-mediated activation of the PI3K/Akt/mTOR pathway in LSCC cells.

Conclusion: Curcumin suppressed LSCC progression through the upregulation of miR-145 and inhibition of the PI3K/Akt/mTOR pathway.
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http://dx.doi.org/10.2147/OTT.S159236DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6016259PMC
June 2018

Coactivator-associated arginine methyltransferase 1 promotes cell growth and is targeted by microRNA-195-5p in human colorectal cancer.

Tumour Biol 2017 Mar;39(3):1010428317694305

Department of General Surgery, Xinhua Hospital, Chongming Branch, School of Medicine, Shanghai Jiao Tong University, Shanghai, P.R. China.

The pathogenesis of colorectal cancer remains poorly understood. Here, we show that coactivator-associated arginine methyltransferase 1 is frequently upregulated in colorectal cancer tissues and promotes cell growth in vitro and in vivo. Using bioinformatics-based prediction and luciferase reporter system, we found that coactivator-associated arginine methyltransferase 1 is post-transcriptionally targeted by microRNA-195-5p in colorectal cancer. Ectopic expression of microRNA-195-5p led to the suppression of the coactivator-associated arginine methyltransferase 1 3'-untranslated regions activity and downregulation of the endogenous coactivator-associated arginine methyltransferase 1 protein in colorectal cancer cells. Expression analysis verified that microRNA-195-5p was markedly downregulated in human colorectal cancer tissues, which was negatively correlated with the elevated levels of coactivator-associated arginine methyltransferase 1 protein. Enhanced levels of microRNA-195-5p in colorectal cancer cells resulted in a sharp reduction of cell proliferative and colony-formative capacities in vitro. Remarkably, restoration of coactivator-associated arginine methyltransferase 1 in microRNA-195-5p-transfected colorectal cancer cells partially abrogated the inhibition of cell proliferation and colony formation mediated through microRNA-195-5p. These data confirm that microRNA-195-5p might function as an anti-tumor microRNA in colorectal cancer exerting critical control over coactivator-associated arginine methyltransferase 1 expression. The newly identified microRNA-195-5p/coactivator-associated arginine methyltransferase 1 axis may act as a novel promising therapeutic target for colorectal cancer treatment.
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http://dx.doi.org/10.1177/1010428317694305DOI Listing
March 2017

Performances of different subset shapes and control points in subset-based digital image correlation and their applications in boundary deformation measurement.

Appl Opt 2015 Feb;54(6):1290-301

Digital image correlation (DIC) is an effective and popular tool for displacement and strain measurements. In the standard subset-based algorithms, the center point of a subset is considered by default as the control point for calculation, and it is difficult to obtain the deformation information at the boundary. Proper selection of the subset shape and the location of control points are vital to the displacement calculation at the boundary. In this paper, registration accuracies of several typical types of subset shapes and different locations of control points are investigated. The results illustrate that different choices of subset shapes can greatly affect the registration accuracy, while different choices for the locations of control points have little impact on it. Based on these results, the noncentral algorithm is developed for the whole-field deformation measurement.
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http://dx.doi.org/10.1364/AO.54.001290DOI Listing
February 2015

Tetrandrine induces apoptosis in gallbladder carcinoma in vitro.

Int J Clin Pharmacol Ther 2014 Oct;52(10):900-5

Objective: The aims of this study were to observe the apoptosis effects of tetrandrine on human gallbladder carcinoma cell line (SGC-996), and to explore its related mechanism.

Methods: First, the anti-proliferative activities of tetrandrine on SGC-996 cells were determined by using the MTT assays. Then, cell cycle changes were detected by flow cytometry analysis. The apoptosis of cells was detected by the annexin V/propidium iodide double-staining assay. Detection of mitochondrial membrane potential was used to validate the ability of tetrandrine on inducing apoptosis. Finally, the expressions of the apoptosis-related proteins (caspase-3, PARP, Bcl-2, and Bax) were analyzed by western blot. Statistical analyses were performed using the Student’s t-test for comparison of the results obtained from cells with or without treatment of tetrandrine.

Results: The MTT assay revealed a significant inhibition of cell proliferation in a dose- and time-dependent manner. Cells treated with tetrandrine were arrested at the S phase, according to the flow cytometric analysis. Tetrandrine produced a dose-dependent increase in the apoptotic cell population compared with control cells. Tetrandrine can also affect mitochondrial function by changing the mitochondrial membrane potential. Furthermore, western blot assay demonstrated that the tetrandrine induced apoptosis in SGC-996 cells by regulating the ratio of Bcl-2/Bax and activating the expression of cleaved caspase-3.

Conclusions: The results indicate that tetrandrine may be a potential agent for the treatment of gallbladder carcinoma.
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http://dx.doi.org/10.5414/CP202123DOI Listing
October 2014

Ultra-performance liquid chromatography-tandem mass spectrometric method for the determination of strychnine and brucine in mice plasma.

J Chromatogr B Analyt Technol Biomed Life Sci 2011 Sep 30;879(26):2714-9. Epub 2011 Jul 30.

Clinical Pharmacy and Pharmacology Research Institute, Second Xiangya Hospital, Central South University, Changsha 410011, People's Republic of China.

A selective, simple and efficient method-ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed for determination of two toxic alkaloids, namely strychnine and brucine in mice plasma. The UPLC separation was carried out using a 1.7 μm BEH C(18) column (50 mm × 2.1 mm) with a mobile phase consisting of methanol:0.1% formic acid (25:75, v/v), hence providing high efficiency, high resolution and excellent peak shape for the analytes and internal standard. The method was validated over the range of 2.48-496.4 ng/ml for strychnine and 2.64-528 ng/ml for brucine, respectively. Intra- and inter-day accuracy ranged from 95.0% to 107.9% for strychnine, 93.4% to 103.3% for brucine, and the precisions were within 13.8%. The extraction recoveries of both the two alkaloids exceed 81.9%. With a simple and minor sample preparation procedure and short run-time (<3 min), the proposed method was applicable for the pharmacokinetic and toxicological analysis of strychnine and brucine in vivo.
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http://dx.doi.org/10.1016/j.jchromb.2011.07.033DOI Listing
September 2011

Comparative studies on the interaction of caffeic acid, chlorogenic acid and ferulic acid with bovine serum albumin.

Spectrochim Acta A Mol Biomol Spectrosc 2010 Oct 5;77(3):680-6. Epub 2010 May 5.

Central South University, Changsha, Hunan 410083, People's Republic China. lishuang

The substitution of the hydrogen on aromatic and esterification of carboxyl group of the phenol compounds plays an important role in their bio-activities. In this paper, caffeic acid (CaA), chlorogenic acid (ChA) and ferulic acid (FA) were selected to investigate the binding to bovine serum albumin (BSA) using UV absorption spectroscopy, fluorescence spectroscopy and synchronous fluorescence spectroscopy. It was found that the methoxyl group substituting for the 3-hydroxyl group of CaA decreased the affinity for BSA and the esterification of carboxyl group of CaA with quinic acid increased the affinities. The affinities of ChA and FA with BSA were more sensitive to the temperature than that of CaA with BSA. Synchronous fluorescence spectroscopy and time-resolved fluorescence indicated that the Stern-Volmer plots largely deviated from linearity at high concentrations and were caused by complete quenching of the tyrosine fluorescence of BSA.
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http://dx.doi.org/10.1016/j.saa.2010.04.026DOI Listing
October 2010

Ultra-performance liquid chromatography-tandem mass spectrometry for the determination of lacidipine in human plasma and its application in a pharmacokinetic study.

J Pharm Biomed Anal 2008 Aug 25;47(4-5):923-8. Epub 2008 Apr 25.

Clinical Pharmacy & Pharmacology Institute, The Second Xiang Ya Hospital, Central South University, Changsha 410011, PR China.

A selective, rapid and sensitive ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-ESI-MS/MS) method was developed and validated for the quantification of lacidipine in human plasma. With nifedipine as an internal standard, sample pretreatment involved a simple liquid-liquid extraction with tert-butyl methyl ether of 1 ml plasma. The analysis was carried out on an Acquity UPLC BEH C18 column (50 mm x 2.1 mm, 1.7 microm) with flow rate of 0.28 ml/min. The mobile phase was 30 mM ammonium acetate buffer-acetonitrile (18:82, v/v, pH 5.5). The detection was performed on a triple-quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) mode via electrospray ionization (ESI). Linear calibration curves were obtained in the concentration range of 0.025-10.000 ng/ml, with a lower limit of quantification of 0.025 ng/ml. The intra- and inter-day precision (R.S.D.) values were below 15% and accuracy (RE) was -12.7% to 11.9% at all QC levels. The method was successfully applied to a clinical pharmacokinetic study of lacidipine in healthy volunteers following oral administration.
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http://dx.doi.org/10.1016/j.jpba.2008.04.018DOI Listing
August 2008

Simultaneous determination of clozapine, olanzapine, risperidone and quetiapine in plasma by high-performance liquid chromatography-electrospray ionization mass spectrometry.

J Chromatogr B Analyt Technol Biomed Life Sci 2004 Apr;802(2):257-62

Clinical Pharmaceutical Research Institute of Second Xiangya Hospital of Central South University, Changsha 410011, PR China.

Unlabelled: Clozapine (CLZ), olanzapine (OLZ), risperidone (RIP) and quetiapine (QTP) have been widely used in the treatment of schizophrenia. However, no study (or little study) has been conducted to determine the four drugs simultaneously by the use of high-performance liquid chromatography-electrospray ionization mass spectrometry (HPLC-MS/ESI).

Objective: To develop a sensitive method for simultaneous determination of CLZ, OLZ, RIP and QTP in human plasma by HPLC-MS/ESI.

Methods: The analytes were extracted twice by ether after samples had been alkalinized. The HPLC separation of the analytes was performed on a MACHEREY-NAGEL C(18) (2.0 mm x 125 mm, 3 microm, Germany) column, using water (formic acid: 2.70 mmol/l, ammonium acetate: 10 mmol/l)-acetonitrile (53:47) as mobile phase, with a flow-rate of 0.16 ml/min. The compounds were ionized in the electrospray ionization (ESI) ion source of the mass spectrometer and were detected in the selected ion recording (SIR) mode.

Results: The calibration curves were linear in the ranges of 20-1000 ng/ml for CLZ and QTP, 1-50 ng/ml for OLZ and RIP, respectively. The average extraction recoveries for all the four analysts were at least above 80%. The methodology recoveries were higher than 91% for the analysts. The intra- and inter-day R.S.D. were less than 15%.

Conclusion: The method is accurate, sensitive and simple for routine therapeutic drug monitoring (TDM) and for the study of the pharmacokinetics of the four drugs.
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http://dx.doi.org/10.1016/j.jchromb.2003.11.037DOI Listing
April 2004
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