Publications by authors named "Rongfang Shen"

5 Publications

  • Page 1 of 1

Current progress and quality of radiomic studies for predicting EGFR mutation in patients with non-small cell lung cancer using PET/CT images: a systematic review.

Br J Radiol 2021 Apr 21:20201272. Epub 2021 Apr 21.

Department of Radiology, The First People's Hospital of Kashi Area, Kashi, Xinjiang, China.

Objectives: To assess the methodological quality of radiomic studies based on positron emission tomography/computed tomography (PET/CT) images predicting epidermal growth factor receptor (EGFR) mutation status in patients with non-small cell lung cancer (NSCLC).

Methods: We systematically searched for eligible studies in the PubMed and Web of Science datasets using the terms "radiomics", "PET/CT", "NSCLC", and "EGFR". The included studies were screened by two reviewers independently. The quality of the radiomic workflow of studies was assessed using the Radiomics Quality Score (RQS). Interclass correlation coefficient (ICC) was used to determine inter rater agreement for the RQS. An overview of the methodologies used in steps of the radiomics workflow and current results are presented.

Results: Six studies were included with sample sizes of 973 ranging from 115 to 248 patients. Methodologies in the radiomic workflow varied greatly. The first-order statistics were the most reproducible features. The RQS scores varied from 13.9 to 47.2%. All studies were scored below 50% due to defects on multiple segmentations, phantom study on all scanners, imaging at multiple time points, cut-off analyses, calibration statistics, prospective study, potential clinical utility, and cost-effectiveness analysis. The ICC results for majority of RQS items were excellent. The ICC for summed RQS was 0.986 [95% confidence interval (CI): 0.898-0.998].

Conclusions:: The PET/CT based radiomics signature could serve as a diagnostic indicator of EGFR mutation status in NSCLC patients. However, the current conclusions should be interpreted with care due to the suboptimal quality of the studies. Consensus for standardization of PET/CT based radiomic workflow for EGFR mutation status in NSCLC patients is warranted to further improve research.

Advances In Knowledge: Radiomics can offer clinicians better insight into the prediction of EGFR mutation status in NSCLC patients, whereas the quality of relative studies should be improved before application to the clinical setting.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1259/bjr.20201272DOI Listing
April 2021

Fabrication and Potential Applications of Highly Durable Superhydrophobic Polyethylene Terephthalate Fabrics Produced by In-Situ Zinc Oxide (ZnO) Nanowires Deposition and Polydimethylsiloxane (PDMS) Packaging.

Polymers (Basel) 2020 Oct 13;12(10). Epub 2020 Oct 13.

CAS Center for Excellence on TMSR Energy System, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, No. 2019 Jialuo Road, Jiading District, Shanghai 201800, China.

Considerable attention has been devoted to the in-situ deposition of zinc oxide (ZnO) nanowires (ZnO-NWs) on the surface of organic supports, due to their very wide applications in superhydrophobicity, UV shielding, and nanogenerators. However, the poor interfacial bond strength between ZnO-NWs and its support limits their applications. Herein, we developed a facile process to grow robust ZnO-NWs on a polyethylene terephthalate (PET) fabric surface through simultaneous radiation-induced graft polymerization, hydrothermal processing, and in-situ nano-packaging; the obtained materials were denoted as PDMS@ZnO-NWs@PET. The introduction of an adhesion and stress relief layer greatly improved the attachment of the ZnO-NWs to the support, especially when the material was subjected to extreme environment conditions of external friction forces, strong acidic or alkaline solutions, UV-irradiation and even washing with detergent for a long time. The PDMS@ZnO-NWs@PET material exhibited excellent UV resistance, superhydrophobicity, and durability. The ZnO-NWs retained on the fabric surface even after 30 cycles of accelerated washing. Therefore, this process can be widely applied as a universal approach to overcome the challenges associated with growing inorganic nanowires on polymeric support surfaces.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/polym12102333DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7600959PMC
October 2020

Identification of Distinct Immune Subtypes in Colorectal Cancer Based on the Stromal Compartment.

Front Oncol 2019 10;9:1497. Epub 2020 Jan 10.

State Key Laboratory of Molecular Oncology, Department of Etiology and Carcinogenesis, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

The tumor environment is of vital importance for the incidence and development of colorectal cancer. Increasing evidence in recent years has elaborated the vital role of the tumor environment in cancer subtype classification and patient prognosis, but a comprehensive understanding of the colorectal tumor environment that is purely dependent on the stromal compartment is lacking. To decipher the tumor environment in colorectal cancer and explore the role of its immune context in cancer classification, we performed a gene expression microarray on the stromal compartment of colorectal cancer and adjacent normal tissues. Through the integrated analysis of our data with public gene expression microarray data of stromal and epithelial colorectal cancer tissues processed through laser capture microdissection, we identified four highly connected gene modules representing the biological features of four tissue compartments by applying a weighted gene coexpression network analysis algorithm and classified colorectal cancers into three immune subtypes by adopting a nearest template prediction algorithm. A systematic analysis of the four identified modules mainly reflected the close interplay between the biological changes of intrinsic and extrinsic characteristics at the initiation of colorectal cancer. Colorectal cancers were stratified into three immune subtypes based on gene templates identified from representative gene modules of the stromal compartment: active immune, active stroma, and mixed type. These immune subtypes differed by the immune cell infiltration pattern, expression of immune checkpoint inhibitors, mutation landscape, extent of mutation burden, extent of copy number burden, prognosis and chemotherapeutic sensitivity. Further analysis indicated that activation of the signaling pathway was the major mechanism causing the no immune infiltration milieu in the active stroma subtype and that inhibitors of the signaling pathway could be candidate drugs for treating patients with an active stroma. Overall, these results suggest that characterizing colorectal cancer by the tumor environment is of vital importance in predicting patients' clinical outcomes and helping guide precision and personalized treatment.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2019.01497DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6965328PMC
January 2020

Co anchored on surface-functionalized PET non-woven fabric and used as high efficiency monoatom-like catalyst for activating Oxone in water.

Sci Total Environ 2020 Jan 5;699:134286. Epub 2019 Sep 5.

CAS Center for Excellence on TMSR Energy System, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, No. 2019 Jialuo Road, Jiading District, Shanghai 201800, China; School of Physical science and Technology, ShanghaiTech University, Shanghai 200031, China. Electronic address:

Fenton-like processes have emerged as most promising techniques for generating reactive oxygen-containing radicals to deal with increasing levels of environmental pollution. Developing novel catalysts with simple manufacturing requirements, excellent activity levels, and stability remains a long-term goal in terms of practical application. So herein, a new polyethylene terephthalate (PET) non-woven fabric based composite catalyst has been fabricated, using radiation-induced graft polymerization of a functionalized group to chelate Co ions as heterogeneous catalysts in peroxymonosulfate (Oxone) activation. Several impact factors, including catalyst dosage, Oxone concentration, reaction temperature, pH value, Co precipitation ratio (of Co@PET at different pH values), and highly concentrated NaCl have been investigated here. Notably, Co@PET has shown the lowest activation energy of any reported catalyst, for degrading RhB by activating Oxone. Interestingly, as experimental RhB and Oxone solutions were passed through single Co@PET sheets, the RhB was decomposed into a colorless solution in the penetration process. Based on radical trapping and quenching experiments, a channel was determined to dominate RhB degradation, and furthermore, Co@PET could be re-used for RhB degradation by activating Oxone. These results showed that Co@PET effectively provided improved Fenton-like catalytic performance and stability, and was suitable for practical applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.scitotenv.2019.134286DOI Listing
January 2020

Immune microenvironments differ in immune characteristics and outcome of glioblastoma multiforme.

Cancer Med 2019 06 30;8(6):2897-2907. Epub 2019 Apr 30.

State Key Laboratory of Molecular Oncology, Department of Etiology and Carcinogenesis, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Understanding the interactions between tumors and the host immune system holds great promise to uncover biomarkers for targeted therapies, predict the prognosis of patients and guide clinical treatment. However, the immune signatures of glioblastoma multiforme (GBM) remain largely unstudied in terms of systematic analyses. We aimed to classify GBM samples according to immune-related genes and complement the existing immunotherapy system knowledge. In this study, we designed a strategy to identify 3 immune subtypes representing 3 different immune microenvironments (M1-M3) and associated with prognosis. The 3 subtypes were significantly different in terms of specific immune characteristics (immune cell subpopulations, immune responses, immune cells, and checkpoint gene interactions). In additional, copy number variations and methylation changes were identified that correlated with genes related to a worse prognosis subtype in the microenvironment. More importantly, in M3 (worst prognosis subtype) and M2 (best prognosis subtype), the interaction between immune cells and checkpoint genes was different, which had an important effect on the prognosis. Finally, we used risk scores of immune cells and checkpoint genes to evaluate the prognosis of GBM patients and validated the results with 3 independent datasets. Disordered interactions between immune cells and checkpoint genes result in a change in the immune microenvironment and affects the prognosis of patients. We propose that a better understanding of the immune microenvironment of advanced cancers may provide new insights into immunotherapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/cam4.2192DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6558448PMC
June 2019