Publications by authors named "Rong Zhang"

2,155 Publications

  • Page 1 of 1

Saliva nitrite is higher in male children with autism spectrum disorder and positively correlated with serum nitrate.

Redox Rep 2021 Dec;26(1):124-133

International Joint Research Center for General Health, Precision Medicine & Nutrition, Hubei University of Technology, Wuhan, People's Republic of China.

Objectives: Nitric oxide (NO) plays a vital role in neurological development. As an easily accessible and non-invasive fluid, saliva hasn't been evaluated for nitrite among children with autism spectrum disorder (ASD). This study aims to quantify saliva nitrite and explore its relation with serum NO.

Methods: Saliva sampling and pretreatment methods were optimized, followed by NO measurement via chemiluminescence for 126 ASD children and 129 normally developing children (ND).

Results: In the ASD group, saliva nitrite was significantly higher than that in the ND, with concentrations of 4.97 ± 3.77 μM and 2.66 ± 2.07 μM ( < 0.0001), respectively. Positive correlation was observed between saliva NO and serum NO in ASD children, which didn't exist in the ND group. Male children in the ASD group had significantly higher NO than that in boys of the ND group, without significant difference between girls in both groups. Correlation was not found between saliva or serum NO and severity of these ASD children.

Discussion: It is reported for the first time that saliva nitrite was positively correlated with serum nitrate in ASD children, with significantly higher NO only in autistic boys. Non-invasive saliva might serve as a predictor of health status of ASD children.
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http://dx.doi.org/10.1080/13510002.2021.1959133DOI Listing
December 2021

Mobile Colistin Resistance Enzyme MCR-3 Facilitates Bacterial Evasion of Host Phagocytosis.

Adv Sci (Weinh) 2021 Jul 29:e2101336. Epub 2021 Jul 29.

Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.

Mobile colistin resistance enzyme MCR-3 is a phosphoethanolamine transferase modifying lipid A in Gram-negative bacteria. MCR-3 generally mediates low-level (≤8 mg L ) colistin resistance among Enterobacteriaceae, but occasionally confers high-level (>128 mg L ) resistance in aeromonads. Herein, it is determined that MCR-3, together with another lipid A modification mediated by the arnBCADTEF operon, may be responsible for high-level colistin resistance in aeromonads. Lipid A is the critical site of pathogens for Toll-like receptor 4 recognizing. However, it is unknown whether or how MCR-3-mediated lipid A modification affects the host immune response. Compared with the wild-type strains, increased mortality is observed in mice intraperitoneally-infected with mcr-3-positive Aeromonas salmonicida and Escherichia coli strains, along with sepsis symptoms. Further, mcr-3-positive strains show decreased clearance rates than wild-type strains, leading to bacterial accumulation in organs. The increased mortality is tightly associated with the increased tissue hypoxia, injury, and post-inflammation. MCR-3 expression also impairs phagocytosis efficiency both in vivo and in vitro, contributing to the increased persistence of mcr-3-positive bacteria in tissues compared with parental strains. This study, for the first time, reveals a dual function of MCR-3 in bacterial resistance and pathogenicity, which calls for caution in treating the infections caused by mcr-positive pathogens.
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http://dx.doi.org/10.1002/advs.202101336DOI Listing
July 2021

Application of traditional Chinese and Western medicine combined with chronic disease management in pulmonary rehabilitation and evaluation of efficacy.

Am J Transl Res 2021 15;13(6):6372-6381. Epub 2021 Jun 15.

Department of Pain, Shanghai Quyang Hospital Hongkou, Shanghai 200092, China.

Objective: This study evaluated the efficacy of traditional Chinese and western medicine combined with chronic disease management on rehabilitation of chronic obstructive pulmonary disease (COPD) patients.

Methods: A total of 199 COPD patients in Shanghai Construction Group (SCG) Hospital were recruited as research objects. The control group (CG) consisted of 100 patients treated with conventional western chronic disease management, and the research group (RG) consisted of 99 patients treated with chronic disease management with combined traditional Chinese and western medicine. The efficacy, pulmonary rehabilitation performance, compliance score, 6-minute walk test (6MWT), modified Medical Research Council dyspnoea scale (MMRC), COPD assessment test (CAT), pulmonary function (PaO, PaCO, FEV1, PEF), self-rating anxiety scale (SAS), self-rating depression scale (SDS) and patient satisfaction between the two groups were compared.

Results: Pulmonary rehabilitation performance, 6MWT results, and patient satisfaction in the RG were significantly better than those in the CG. The total effective rate, compliance score, PaO, FEV1 and PEF of the RG were significantly higher than those of the CG. After treatment, the COPD symptom score, CAT score, PaCO, SAS score and SDS score in the RG were significantly lower than those in the CG.

Conclusion: Chronic disease management with combined traditional Chinese and western medicine has great application value and high efficacy in pulmonary rehabilitation.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290697PMC
June 2021

Activatable Rare Earth Near-Infrared-II Fluorescence Ratiometric Nanoprobes.

Nano Lett 2021 Jul 24. Epub 2021 Jul 24.

School of Nano-Tech and Nano-Bionics, University of Science and Technology of China, Hefei 230026, China.

Rational design of efficient lanthanide-doped down-shifting nanoparticles (DSNPs) has attracted tremendous attention. However, energy loss was inevitable in the multiple Ln doping systems owing to complex energy migration processes. Here, an efficient [email protected]@NaYF:10%[email protected] DSNP was tactfully designed, in which a buffer layer of NaYF was modulated to restrict the interionic energy migration between Er and Nd; meanwhile, the surface defects were passivated by an outermost layer of NaYF. Therefore, the as-prepared DSNPs exhibited two intensive near-infrared-II fluorescence emissions of 1525 nm from Er and 1060 nm from doped Nd under 808 nm excitation. Further, a novel ratiometric nanoprobe [email protected]@NaYF:10%[email protected]@A1094 was fabricated by coupling an organic dye of A1094 onto the DSNP surface to quench the 1060 nm emission by the efficient Förster resonance energy transfer, while emission at 1525 nm retained. Thereafter, these activatable ratiometric nanoprobes were used for rapid and sensitive detection of peroxynitrite (ONOO) in vivo.
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http://dx.doi.org/10.1021/acs.nanolett.1c01962DOI Listing
July 2021

Genome-Wide Identification of Gene Family in Pitaya Reveals an Involved in Flowering Process.

Int J Mol Sci 2021 Jul 19;22(14). Epub 2021 Jul 19.

Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou 510642, China.

Aquaporins (AQPs) are essential membrane proteins involved in seed maturation and germination, stomata movement, photosynthesis, and regulation of plant flowering processes. Pitaya flowers are open at night and wither at daybreak, which shows an obvious circadian rhythm. In this study, a comprehensive genome-wide analysis of in was conducted to screen key genes associated with flowering processes. A total of 33 genes were identified from the genome. The 33 were grouped into four subfamilies: 10 PIPs, 13 TIPs, 8 NIPs, and 2 SIPs, which were distributed on 9 out of 11 pitaya chromosomes (Chr) (except for Chr7 and Chr10). Results from expression profiles showed that may be involved in pitaya's floral opening. was localized exclusively in the cell membrane. Overexpression of in significantly promoted early flowering through regulating negative flowering regulators of , , and , suggesting that plays key roles in regulating flowering time. The present study provides the first genome-wide analysis of the gene family in pitaya and valuable information for utilization of .
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http://dx.doi.org/10.3390/ijms22147689DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8306030PMC
July 2021

Recombinant chimpanzee adenovirus AdC7 expressing dimeric tandem-repeat spike protein RBD protects mice against COVID-19.

Emerg Microbes Infect 2021 Jul 22:1-40. Epub 2021 Jul 22.

Key Laboratory of Tropical Translational Medicine of Ministry of Education, School of Tropical Medicine and Laboratory Medicine, The First Affiliated Hospital, Hainan Medical University, Haikou, China.

A safe and effective vaccine is urgently needed to control the unprecedented COVID-19 pandemic. Four adenovirus vectored vaccines expressing spike (S) protein have been approved for use. Here, we generated several recombinant chimpanzee adenovirus (AdC7) vaccines expressing S, receptor-binding domain (RBD) or tandem-repeat dimeric RBD (RBD-tr2). We found vaccination via either intramuscular or intranasal route was highly immunogenic in mice to elicit both humoral and cellular immune responses. AdC7-RBD-tr2 showed higher antibody responses compared to either AdC7-S or AdC7-RBD. Intranasal administration of AdC7-RBD-tr2 additionally induced mucosal immunity with neutralizing activity in bronchoalveolar lavage fluid. Either single-dose or two-dose mucosal administration of AdC7-RBD-tr2 protected mice against SARS-CoV-2 challenge, with undetectable subgenomic RNA in lung and relieved lung injury. AdC7-RBD-tr2-elicted sera preserved the neutralizing activity against the circulating variants, especially the Delta variant. These results support AdC7-RBD-tr2 as a promising COVID-19 vaccine candidate.
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http://dx.doi.org/10.1080/22221751.2021.1959270DOI Listing
July 2021

Molecular mechanism associated with the use of magnetic fermentation in modulating the dietary lipid composition and nutritional quality of goat milk.

Food Chem 2021 Jul 7;366:130554. Epub 2021 Jul 7.

School of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi'an 710021, China; Shaanxi Research Institute of Agricultural Products Processing Technology, Xi'an 710021, China. Electronic address:

Standard fermentation (SF) mainly affected the metabolism of glycerophospholipid and sphingolipid, and increased the total lipid content of goat milk. Content of total lipid was decreased by magnetic fermentation compared with SF, mainly due to triacylglycerol and diacylglycerol. Comprehensive characteristic of lipids dynamic changes during standard and magnetic fermentation was performed using high-throughput quantitative lipidomics. Totally, 488 lipid molecular species covering 12 subclasses were detected, and triacylglycerol was the highest levels, followed by diacylglycerol and phosphoethanolamine in the whole fermentation stage. Specifically, except for ceramide and simple Glc series, the content of all polar lipids in SF was dropped and neutral lipids subjoined. Compared with SF, the decrease of triacylglycerol (1752.47 to 784.78 μg/mL), diacylglycerol (60.36 to 24.89 μg/mL) and simple Glc series (4.36 to 2.40 μg/mL) were observed, while ceramide (6.54 to 25.87 μg/mL) increased, suggesting magnetic fermentation as effective approach to potentially improve the nutritional of goat milk.
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http://dx.doi.org/10.1016/j.foodchem.2021.130554DOI Listing
July 2021

Mitochondria damage in ambient particulate matter induced cardiotoxicity: Roles of PPAR alpha/PGC-1 alpha signaling.

Environ Pollut 2021 Jul 14;288:117792. Epub 2021 Jul 14.

Department of Toxicology, School of Public Health, Qingdao University, Qingdao, China. Electronic address:

Particulate matter (PM) had been associated with cardiotoxicity, while the mechanism of toxicity has yet to be elucidated, with mitochondria dysfunction as a potential candidate. To investigate the potential cardiotoxic effects of ambient PM exposure and assess the damage to cardiac mitochondria, C57/B6 mice were exposed to filtered air or real ambient PM for three or six weeks. Furthermore, to reveal the role of peroxisome proliferators-activated receptor alpha (PPAR alpha) in PM exposure induced cardiotoxicity/mitochondria damage, animals were also co-treated with PPAR alpha agonist WY 14,643 or PPAR alpha antagonist GW 6471. Cardiotoxicity was assessed with echocardiography and histopathology, while mitochondria damage was evaluated with mitochondria membrane potential measurement and transmission electron microscopy. Potential impacts of PM exposure to PPAR alpha signaling were detected with co-immunoprecipitation and western blotting. The results indicated that exposure to ambient PM exposure induced cardiotoxicity in C57/B6 mice, including altered cardiac functional parameters and morphology. Cardiac mitochondria damage is detected, in the form of compromised mitochondria membrane potential and morphology. Molecular investigations revealed disruption of PPAR alpha interaction with peroxisome proliferator-activated receptor gamma coactivator-1A (PGC-1a) as well as altered expression levels of PPAR alpha downstream genes. Co-treatment with WY 14,643 alleviated the observed toxicities, while co-treatment with GW 6471 had mixed results, exaggerating most cardiotoxicity and mitochondrial damage endpoints but alleviating some cardiac functional parameters. Interestingly, WY 14,643 and GW 6471 co-treatment seemed to exhibit similar regulative effects towards PPAR alpha signaling in animals exposed to PM. In conclusion, ambient PM exposure indeed induced cardiotoxicity in C57/B6 mice, in which cardiac mitochondria damage and disrupted PPAR alpha signaling are contributors.
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http://dx.doi.org/10.1016/j.envpol.2021.117792DOI Listing
July 2021

Study of membrane fouling mechanism during the phenol degradation in microbial fuel cell and membrane bioreactor coupling system.

Bioresour Technol 2021 Jul 5;338:125504. Epub 2021 Jul 5.

School of the Environment and Safety Engineering, North University of China, Taiyuan 030051, China. Electronic address:

This study evaluated the feasibility of phenol degradation in microbial fuel cell (MFC) and membrane bioreactor (MBR) coupling system, and explored the mechanism of MBR membrane fouling. Four aspects were researched in open and closed circuit conditions: the degradation capacity of the coupling system, the increase of trans-membrane pressure (TMP), and the adhesion of phenol degradation products and microorganisms on the membrane. The results showed that the degradation of phenol and COD in the closed circuit coupling system was higher than that in the open circuit. The micro-electric field can inhibit the growth of TMP and keep dodecamethylcyclohexasiloxane away from the membrane, meanwhile can also reduce the abundance and species diversity of microorganisms. Nevertheless, the micro-electric field could not completely eliminate the membrane fouling due to the fact that the phenol degradation product of ethanethiol, microorganisms of Proteobacteria and Actinobacteria were more favorable on the membrane.
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http://dx.doi.org/10.1016/j.biortech.2021.125504DOI Listing
July 2021

A systematic review and meta-analysis: effects of glucocorticoids on rheumatoid arthritis and systemic lupus erythematosus.

Ann Palliat Med 2021 Jul 8. Epub 2021 Jul 8.

Department of Rheumatology and Immunology, The First Hospital of China Medical University, Shenyang, China.

Background: Meta-analysis was performed to explore the efficacy of glucocorticoids in the treatment of rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE), to provide a theoretical basis for the clinical treatment of patients.

Methods: Relevant literatures from the establishment of the database to December 31, 2020, were searched from databases such as PubMed. The literatures with randomized controlled trial of the clinical efficacy of glucocorticoids in the treatment of RA and SLE were screened for meta-analysis.

Results: Eleven documents were included, including 1,298 participants. It was found that the cardiovascular system [mean difference (MD) =1.23; 95% confidence interval (CI): 0.64 to 2.34; Z=0.62; P=0.53], respiratory system (MD =1.87; 95% CI: -0.66 to 5.29; Z=1.18; P=0.24), nervous system (MD =1.22; 95% CI: 0.25-5.84; Z=0.25; P=0.8), visual impairment (MD =1.41; 95% CI: 0.79-2.52; Z=1.15; P=0.25), endocrine system (MD =8.53; 95% CI: 2.71-26.88; Z=3.66; P=0.0003), digestive system (MD =1.41; 95% CI: 0.76- 2.63; Z=1.09; P=0.28), genitourinary system (MD =1.06; 95% CI: 0.35-3.17; Z=0.1; P=0.92), blood system (MD =2.96; 95% CI: 0.62-14.26; Z=1.35; P=0.18), Z=0.48; P=0.63), infection status (MD =1.36; 95% CI: 0.98-1.87; Z=1.86; P=0.06), clinical efficacy (MD =1.79; 95% CI: 1.27-2.52; Z=3.32; P=0.0009), pain (MD =1.16; 95% CI: 0.76-1.78; Z=0.68; P=0.5), and joint swelling score (MD =0.03; 95% CI: -0.38 to 0.45; Z=0.15; P=0.88) of experimental group after treatment were all superior versus controls. However, the skin and mucous membranes (MD =0.87; 95% CI: 0.55-1.37; Z=0.61; P=0.54), musculoskeletal (MD =0.85; 95% CI: 0.43-1.66; Z=0.48; P=0.63), radiation injury (MD =-1.93; 95% CI: -3.68 to -0.18; Z=2.17; P=0.03), and C-reactive protein (CRP) level (MD =-8.66; 95% CI: -10.16 to -7.16; Z=11.34; P<0.00001) of experimental group were inferior to those of control group.

Conclusions: Glucocorticoids in the treatment of RA and SLE can improve the clinical efficacy, but it was easy to cause multiple system adverse reactions. Therefore, the clinical treatment should follow the doctor's advice.
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http://dx.doi.org/10.21037/apm-21-1485DOI Listing
July 2021

Mutations of contribute to the pathogenesis of non-alcoholic fatty liver disease and related metabolic disorders.

Diabetes 2021 Jul 14. Epub 2021 Jul 14.

Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Clinical Centre for Diabetes, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.

Neuregulin 4 (Nrg4), an adipose tissue-enriched endocrine factor, participates in adipocyte-to-hepatocyte communication, eliciting beneficial metabolic effects in non-alcoholic fatty liver disease (NAFLD). Here, we evaluate the physiological roles of Nrg4 in humans and unravel the role of variants in the pathogenesis of NAFLD and related metabolic disorders. We identified two rare missense mutations-p. R44H and p.E47Q- in the NRG4 EGFL domain by whole exome sequencing in 224 severely obese subjects and exome genotyping in 2,388 subjects from Shanghai Obesity Study. The over-expression animal models showed wild-type (WT) Nrg4 could attenuate high-fat diet-induced hepatic lipogenesis and improve energy metabolism. Nrg4 E47Q enhanced the protective effect, whereas Nrg4 R44H lost this function. Unlike Nrg4 R44H, Nrg4 E47Q activated the phosphorylation of ErbB4 and negatively regulated the lipogenesis via the ErbB4-STAT5-SREBP1C pathway. The surface plasmon resonance experiments revealed a higher affinity of E47Q Nrg4 than WT to bind ErbB4, while R44H showed no binding. In conclusion, the study suggests that genetic variations in could produce mutant proteins with aberrant functions, and impaired or enhanced Nrg4 function could either be a risk factor or protective factor for NAFLD and associated metabolic disorders.
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http://dx.doi.org/10.2337/db21-0064DOI Listing
July 2021

Characteristics of Three Person-to-Person Transmission Clusters of Severe Fever with Thrombocytopenia Syndrome in Southeastern China.

Am J Trop Med Hyg 2021 Jul 12. Epub 2021 Jul 12.

Key Laboratory of Vaccine, Prevention and Control of Infectious Disease of Zhejiang Province, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China.

Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease worldwide. It can be transmitted from person to person, and the fatality rate is very high. During this study, three SFTS clusters including 12 associated cases were identified in three counties in Zhejiang Province from 2018 to 2020. The median age of the three index patients was 70 years, and that of secondary case patients was 59 years. Of note, the mortality rate of the index patients was 100%. The mortality rate of secondary case patients was 11%. The total secondary attack rate (SAR) was 30% (9/30). The SARs of cluster A, cluster B, and cluster C were 38% (3/8), 21% (3/14), and 38% (3/8), respectively. Additionally, the interval from onset to diagnosis was 4 days. The intervals from disease onset to confirmation of the index cases and secondary cases were 7 days and 4 days, respectively. All secondary case patients had a history of close contact with blood or body fluids of the index patients. These results indicate that SFTS patients should not be discharged until recovery. When SFTS patients die, the corpses should be transferred directly from the hospital to the crematorium for cremation by persons wearing proper protective equipment to prevent virus transmission.
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http://dx.doi.org/10.4269/ajtmh.21-0366DOI Listing
July 2021

miR-19a-3p downregulates tissue factor and functions as a potential therapeutic target for sepsis-induced disseminated intravascular coagulation.

Biochem Pharmacol 2021 Jul 8:114671. Epub 2021 Jul 8.

The Key Laboratory for Human Disease Gene Study of Sichuan Province, Prenatal Diagnosis Center, Sichuan Academy of Medical Sciences&Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, No.32, West section2, 1st ring road, Qingyang District, Chengdu, Sichuan, 610072, China. Electronic address:

Sepsis-induced disseminated intravascular coagulation (DIC) is a common life-threatening terminal-stage disease with high mortality. This study aimed to identify effective miRNAs as therapeutic targets for DIC.In the present study, bioinformatics and luciferase reporter gene analyses were performed to predict miR-19a-3p and validate that it targets tissue factor (TF). Quantitative real-time PCR was used to detect the expression of miR-19a-3p and TF, and TF procoagulant activity was determined by chromogenic substrate method.Western blotting was used to detect the protein levels of TF, AKT, Erk1/2, NF-κB P65, IκB-a and their phosphorylated counterparts in cell experiments. Furthermore, a rat model was established to explore the potential of miR-19a-3p in DIC treatment. As a result, the human clinical study revealed that miR-19a-3p was downregulated and that TF was upregulated in neonates with sepsis-induced DIC compared with control group. The luciferase reporter assay showed that TF was a direct target of miR-19a-3p. Cell experiments verified that the mRNA and protein levels of TF, and the p-AKT/AKT, p-Erk/Erk, p-P65/P65, p-IκB/IκB-a ratios, and TF procoagulant activity were significantly decreased in lipopolysaccharide-induced human peripheral blood mononuclear cells and human umbilical vein endothelial cells inhibiting by overexpression of miR-19a-3p. miR-19a-3p regulating TF involved with NF-kB pathway and ATK pathway. In vivo, miR-19a-3p injection into DIC rats suppressed the mRNA of TF, and More importantly, significant improvements in coagulation function indicators and in histopathologies of lung and kidney were observed.In conclusion, miR-19a-3p may suppress DIC via targeting TF and might be a potential therapeutic target in treating sepsis-induced DIC.
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http://dx.doi.org/10.1016/j.bcp.2021.114671DOI Listing
July 2021

Public mental health problems during COVID-19 pandemic: a large-scale meta-analysis of the evidence.

Transl Psychiatry 2021 07 9;11(1):384. Epub 2021 Jul 9.

Department of Medical Psychology, Army Medical University, Chongqing, China.

The coronavirus disease 2019 (COVID-19) pandemic has exposed humans to the highest physical and mental risks. Thus, it is becoming a priority to probe the mental health problems experienced during the pandemic in different populations. We performed a meta-analysis to clarify the prevalence of postpandemic mental health problems. Seventy-one published papers (n = 146,139) from China, the United States, Japan, India, and Turkey were eligible to be included in the data pool. These papers reported results for Chinese, Japanese, Italian, American, Turkish, Indian, Spanish, Greek, and Singaporean populations. The results demonstrated a total prevalence of anxiety symptoms of 32.60% (95% confidence interval (CI): 29.10-36.30) during the COVID-19 pandemic. For depression, a prevalence of 27.60% (95% CI: 24.00-31.60) was found. Further, insomnia was found to have a prevalence of 30.30% (95% CI: 24.60-36.60). Of the total study population, 16.70% (95% CI: 8.90-29.20) experienced post-traumatic stress disorder (PTSD) symptoms during the COVID-19 pandemic. Subgroup analysis revealed the highest prevalence of anxiety (63.90%) and depression (55.40%) in confirmed and suspected patients compared with other cohorts. Notably, the prevalence of each symptom in other countries was higher than that in China. Finally, the prevalence of each mental problem differed depending on the measurement tools used. In conclusion, this study revealed the prevalence of mental problems during the COVID-19 pandemic by using a fairly large-scale sample and further clarified that the heterogeneous results for these mental health problems may be due to the nonstandardized use of psychometric tools.
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http://dx.doi.org/10.1038/s41398-021-01501-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8266633PMC
July 2021

EEG Spectral Power: A Proposed Physiological Biomarker to Classify the Hypoxic-Ischemic Encephalopathy Severity in Real Time.

Pediatr Neurol 2021 Jun 12;122:7-14. Epub 2021 Jun 12.

Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas. Electronic address:

Background: Mild hypoxic-ischemic encephalopathy (HIE) constitutes a large unstudied population with considerable debate on how to define and treat due to the dynamic evolution of the clinical signs of encephalopathy. We propose to address this gap with quantitative physiological biomarkers to aid in stratification of the disease severity. The objectives of this prospective cohort study were to measure the electroencephalographic (EEG) power as an objective biomarker of the evolution of the clinical encephalopathy in newborns with mild to severe HIE.

Methods: EEG was collected in infants with HIE using four bipolar electrodes analyzed for the first three hours of the recording. Delta power (DP, 0.5 to 4 Hz) and total power (TP, 0.5 to 20 Hz) were compared between groups with different HIE severity using a univariate ordinal logistic regression model and receiver operating characteristic curves.

Results: A total of 44 term-born infants with mild to severe HIE were identified within six hours of birth. The DP and TP values were significantly higher for the mild group than for the moderate group for all bipolar electrodes. A one-unit increase in DP was associated with significantly lower odds of encephalopathy. DP best distinguished mild from higher encephalopathy grades by area under the curve.

Conclusions: We conclude that DP and TP are sensitive real-time biomarkers for monitoring the dynamic evolution of the encephalopathy severity in the first day of life. The quantitative EEG power may lead to timely recognition of the worsening of the encephalopathy and guide future therapeutic interventions targeting mild HIE.
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http://dx.doi.org/10.1016/j.pediatrneurol.2021.06.001DOI Listing
June 2021

Effects of long-term high-altitude exposure on fibrinolytic system.

Hematology 2021 Dec;26(1):503-509

Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College, Chengdu, People's Republic of China.

Objective: High altitude (HA), with the main feature of hypobaric hypoxia, is an independent risk factor for thrombosis. However, little is known on the alterations of fibrinolytic system in adaptation to HA. In this study, we investigated changes of fibrinolytic system parameters between individuals permanently living at HA and low altitude (LA) regions, and provided data for further studies on HA-induced thrombotic disease.

Material And Methods: A total of 226 eligible participants, including 103 LA participants, 100 healthy HA subjects and 23 high altitude polycythemia (HAPC) patients, were recruited in this study. Six fibrinolytic parameters, i.e. fibrinogen (Fbg), D-dimer (DDi), antithrombin III (AT-III), plasminogen activator inhibitor-1 (PAI-1), tissue plasminogen activator (tPA) and plasminogen (PLG) were analyzed respectively. PAI-1 and tPA were performed by using bio-immuno-assays and an automated coagulation analyzer was used to conduct Fbg, DDi, AT-III and PLG tests.

Results: Plasma levels of Fbg, DDi, PAI-1 and PLG were significantly higher in healthy HA group than in LA group (all < 0.05), whereas tPA was significantly lower in healthy HA group. No significant difference in AT-III was observed between healthy HA and LA groups ( > 0.05). All these fibrinolytic parameters showed no significant distinctions between healthy HA subjects and HAPC patients (all  > 0.05). HGB showed no relationship with fibrinolytic parameters in HA cohort.

Conclusion: This study demonstrates that HA environment has a significant effect on fibrinolytic system and provides a foundation for further studies on HA hypobaric hypoxia-induced thrombotic disease.
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http://dx.doi.org/10.1080/16078454.2021.1946265DOI Listing
December 2021

Discovery of a potent and selective inhibitor of histone lysine demethylase KDM4D.

Eur J Med Chem 2021 Jun 26;223:113662. Epub 2021 Jun 26.

State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Sichuan, 610041, China. Electronic address:

Histone lysine demethylase 4D (KDM4D) plays an important role in the regulation of tumorigenesis, progression and drug resistance and has been considered a potential target for cancer treatment. However, there is still a lack of potent and selective KDM4D inhibitors. In this investigation, we report a new class of KDM4D inhibitors containing the 2-(aryl(pyrrolidine-1-yl)methyl)phenol scaffold, identified through AlphaLisa-based screening, structural optimization, and structure-activity relationship analyses. Among these inhibitors, 24s was the most potent, with an IC value of 0.023 ± 0.004 μM. This compound exhibited more than 1500-fold selectivity towards KDM4D versus KDM4A as well as other JMJD subfamily members, indicating good selectivity for KDM4D. Kinetic analysis indicated that 24s did not occupy the 2-oxoglutarate binding pocket. In an in vitro assay, 24s significantly suppressed the proliferation and migration of colorectal cancer (CRC) cells. Overall, this study has identified a good tool compound to explore the biological function of KDM4D and a good lead compound for drug discovery targeting KDM4D.
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http://dx.doi.org/10.1016/j.ejmech.2021.113662DOI Listing
June 2021

Facile preparation of a superamphiphilic nitrocellulose membrane enabling on-demand and energy-efficient separation of oil/water mixtures and emulsions by prewetting.

Biomater Sci 2021 Jul 8. Epub 2021 Jul 8.

Ministry of Education Key Laboratory for the Green Preparation and Application of Functional Materials, School of Materials Science & Engineering and Hubei Key Laboratory of Polymer Materials, Hubei University, Wuhan 430062, People's Republic of China. and State Key Laboratory of Solid Lubrication, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, People's Republic of China.

A membrane with superamphiphilicity presents many advantages in various oil/water separation applications due to its switchable wettability by prewetting. However, it is still a great challenge to switch between two types of superwettability on a single cellulose surface by switching between different liquid media. Herein, in order to obtain in-air superamphiphilic and under-liquid dual superlyophobic membranes, dopamine-modified nitrocellulose membranes (with a pore size of 0.22 μm) were prepared via a facile immersion modification approach. Under 0.08 MPa, the as-prepared NC membrane switches wettability by prewetting to achieve on-demand oil/water separation, and the separation efficiency is more than 99.9%. Futhermore, the membrane prepared in this work can also be applied to high-efficiency on-demand separation of surfactant-stabilized emulsions with a separation efficiency greater than 99.0%. Hence, the PDA-modified NC membrane is a promising controllable oil/water separation material in terms of repeatable cycles, separation efficiency, flux, prominent long-term durability and anti-oil fouling.
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http://dx.doi.org/10.1039/d1bm00521aDOI Listing
July 2021

Computed tomography radiomics to predict EBER positivity in Epstein-Barr virus-associated gastric adenocarcinomas: a retrospective study.

Acta Radiol 2021 Jul 7:2841851211029083. Epub 2021 Jul 7.

Department of Radiology, Sun Yat-sen University Cancer Center; State Key Laboratory of Oncology in Southern China, Guangzhou, PR China.

Background: The relevance of Epstein-Barr virus (EBV) in gastric carcinoma has been represented by the existence of EBV-encoded small RNA (EBER) in the tumor cells and has prognostic significance in gastric cancer, while gastric adenocarcinoma represents the most frequently occurring gastric malignancy.

Purpose: To observe the capacity of radiomic features extracted from contrast-enhanced computed tomography (CE-CT) images to differentiate EBER-positive gastric adenocarcinoma from EBER-negative ones.

Material And Methods: A total of 54 patients with gastric adenocarcinoma (EBER-positive: 27, EBER-negative: 27) were retrospectively examined. Radiomic imaging features were extracted from all regions of interest (ROI) delineated by two experienced radiologists on late arterial phase CT images. We distinguished related radiomic features through the two-tailed t test and applied them to construct a decision tree model to evaluate whether EBER in situ hybridization positive had appeared.

Results: Nine radiomics features were significantly related to EBER in situ hybridization status ( < 0.05), four of which were used to build the decision tree through backward elimination: Correlation_ AllDirection_offset7, Correlation_ angle135_offset7, RunLengthNonuniformity_ AllDirection_offset1_SD, and HighGreyLevelRunEmphasis_ AllDiretion_offset1_SD. The decision tree model consisted of seven decision nodes and six terminal nodes, three of which demonstrated positive EBER in situ hybridization. The specificity, sensitivity, and accuracy of the model were 84%, 80%, and 81.7%, respectively. The area under the curve of the decision tree model was 0.87.

Conclusion: Radiomics based on CE-CT could be applied to predict EBER in situ hybridization status preoperatively in patients with gastric adenocarcinoma.
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http://dx.doi.org/10.1177/02841851211029083DOI Listing
July 2021

Characterization and molecular analysis of the viable but nonculturable state of a yeast sp. strain LN1 induced by high phenol concentrations.

Appl Environ Microbiol 2021 Jul 7:AEM0111021. Epub 2021 Jul 7.

College of Geography and Environmental Science, Zhejiang Normal University, Jinhua 321004, China.

Microbial degradation plays an important role in the environmental remediation. However, most microorganisms' pollutant-degrading capability is weakened due to their entry into a viable but non-culturable (VBNC) state. Despite there is some evidence for the VBNC state of pollutant-degrading bacteria, limited studies have been conducted to investigate the VBNC state of pollutant degraders among fungi. In this work, the morphological, physiological and molecular changes of a phenol-degrading yeast strain LN1 exposed to high phenol concentrations were investigated. The results confirmed that sp. strain LN1, which possessed highly efficient capability in degrading 1000 mg/L of phenol, as well as high potential for aromatic compounds degradation, entered into the VBNC state after 14 h of incubation at 6000 mg/L phenol. Resuscitation of VBNC cells can restore their phenol degradation performance. Compared to normal cells, significant dwarfing, surface damage and physiological changes of VBNC cells were observed. Molecular analysis indicated that the down-regulated genes were related to oxidative stress response, xenobiotics degradation, carbohydrate and energy metabolism, whereas the up-regulated genes were related to RNA polymerase, amino acid metabolism, DNA replication and repair. This report revealed that a pollutant-degrading yeast strain entered into the VBNC state under high concentrations of contaminants, providing new insights into its survival status and bioremediation potential under stress. The viable but non-culturable (VBNC) state is known to affect the culturability and activity of microorganisms. However, limited studies have been conducted to investigate the VBNC state of other pollutant degraders such as fungi. In this study, the VBNC state of a phenol-degrading yeast strain was discovered. In addition, comprehensive analysis of the morphological, physiological and molecular changes of the VBNC cells were performed. This study provides new insight into the VBNC state of pollutant degraders, and how they restored the activities that was inhibited by stressful conditions. Enhanced bioremediation performance of indigenous microorganisms could be expected by preventing and controlling the formation of VBNC state.
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http://dx.doi.org/10.1128/AEM.01110-21DOI Listing
July 2021

Consecutive Monitoring of Interleukin-6 Is Needed for COVID-19 Patients.

Virol Sin 2021 Jul 7. Epub 2021 Jul 7.

State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, 430071, China.

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http://dx.doi.org/10.1007/s12250-021-00425-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261036PMC
July 2021

Triglyceride-Glucose Index and Extracellular Volume Fraction in Patients With Heart Failure.

Front Cardiovasc Med 2021 17;8:704462. Epub 2021 Jun 17.

Department of Radiology, Shunde Hospital, Southern Medical University, Foshan, China.

The triglyceride-glucose (TyG) index had been proposed as a reliable surrogate marker of insulin resistance. We aimed to evaluate the association between TyG index and myocardial fibrosis, which was quantified by extracellular volume (ECV) fraction using cardiovascular magnetic resonance (CMR) examination, and their prognostic value in patients with heart failure (HF). In this retrospective cohort study, 103 hospitalized HF patients were included. ECV fraction was calculated using CMR measurements and T1 mapping. TyG index was calculated using fasting triglyceride and blood glucose. The primary outcome events were defined as all-cause mortality and HF hospitalization during follow-up. During the median follow-up of 12.3 months, 39 patients (37.9%) experienced primary outcome events and had higher levels of TyG index, N-terminal pro-B-type natriuretic peptide (NT-proBNP), and ECV fraction compared with those without events. Multivariate linear regression analysis showed that the TyG index was the significant factor determined for ECV fraction ( = 0.36, = 0.01). In multivariate Cox regression analysis, presence of diabetes [hazard ratio (HR) = 1.28, 95% confidence interval (CI) = 1.01-1.62], higher TyG index (HR = 2.01, 95% CI = 1.03-4.01), ECV fraction (HR = 1.73, 95% CI = 1.04-2.88), and NT-proBNP (HR = 2.13, 95% CI = 1.08-4.20) were independent risk factors for the primary outcome events. TyG index is a novel biomarker of myocardial fibrosis in HF patients and can be considered as a useful risk stratification metric in the management of HF.
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http://dx.doi.org/10.3389/fcvm.2021.704462DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8247445PMC
June 2021

Combining Metagenomic Sequencing With Whole Exome Sequencing to Optimize Clinical Strategies in Neonates With a Suspected Central Nervous System Infection.

Front Cell Infect Microbiol 2021 18;11:671109. Epub 2021 Jun 18.

Department of Neonatology, Children's Hospital, Fudan University, Shanghai, China.

Objectives: Central nervous system (CNS) infection has a high incidence and mortality in neonates, but conventional tests are time-consuming and have a low sensitivity. Some rare genetic diseases may have some similar clinical manifestations as CNS infection. Therefore, we aimed to evaluate the performance of metagenomic next-generation sequencing (mNGS) in diagnosing neonatal CNS infection and to explore the etiology of neonatal suspected CNS infection by combining mNGS with whole exome sequencing (WES).

Methods: We prospectively enrolled neonates with a suspected CNS infection who were admitted to the neonatal intensive care unit(NICU) from September 1, 2019, to May 31, 2020. Cerebrospinal fluid (CSF) samples collected from all patients were tested by using conventional methods and mNGS. For patients with a confirmed CNS infection and patients with an unclear clinical diagnosis, WES was performed on blood samples.

Results: Eighty-eight neonatal patients were enrolled, and 101 CSF samples were collected. Fourty-three blood samples were collected for WES. mNGS showed a sample diagnostic yield of 19.8% (20/101) compared to 4.95% (5/101) for the conventional methods. In the empirical treatment group, the detection rate of mNGS was significantly higher than that of conventional methods [27% vs. 6.3%, p=0.002]. Among the 88 patients, 15 patients were etiologically diagnosed by mNGS alone, five patients were etiologically identified by WES alone, and one patient was diagnosed by both mNGS and WES. Twelve of 13 diagnoses based solely on mNGS had a likely clinical effect. Six patients diagnosed by WES also experienced clinical effect.

Conclusions: For patients with a suspected CNS infections, mNGS combined with WES might significantly improve the diagnostic rate of the etiology and effectively guide clinical strategies.
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http://dx.doi.org/10.3389/fcimb.2021.671109DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8253254PMC
July 2021

Virulence alterations in staphylococcus aureus upon treatment with the sub-inhibitory concentrations of antibiotics.

J Adv Res 2021 07 23;31:165-175. Epub 2021 Jan 23.

Department of Microbiology, College of Basic Medical Sciences, Army Medical University, Key Laboratory of Microbial Engineering under the Educational Committee in Chongqing, Chongqing 400038, PR China.

Background: The treatment of patients with infections mainly relies on antistaphylococcal regimens that are established with effective antibiotics. In antibiotic therapy or while living in nature, pathogens often face the sub-inhibitory concentrations (sub-MICs) of antibiotics due to drug pharmacokinetics, diffusion barriers, waste emission, resistant organism formation, and farming application. Different categories of antibiotics at sub-MICs have diverse effects on the physiological and chemical properties of microorganisms. These effects can result in virulence alterations. However, the mechanisms underlying the actions of antibiotics at sub-MICs on virulence are obscure.

Aim Of Review: In this review, we focus on the effects of sub-MICs of antibiotics on virulence from the aspects of cell morphological change, virulence factor expression, bacterial adherence and invasion, staphylococcal biofilm formation, and small-colony variant (SCV) production. The possible mechanisms of antibiotic-induced virulence alterations are also addressed.

Key Scientific Concepts Of Review: Five main aspects of bacterial virulence can be changed in exposure to the sub-MIC levels of antibiotics, resulting in deformed bacterial cells to stimulate abnormal host immune responses, abnormally expressed virulence factors to alter disease development, changed bacterial adhesion and invasion abilities to affect colonization and diffusion, altered biofilm formation to potentate material-related infections, and increased SCV formation to achieve persistent infection and recurrence. These advanced findings expand our knowledge to rethink the molecular signaling roles of antibiotics beyond their actions as antimicrobial agents.
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http://dx.doi.org/10.1016/j.jare.2021.01.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8240104PMC
July 2021

Effects of Kangaroo Mother Care on Repeated Procedural Pain and Cerebral Oxygenation in Preterm Infants.

Am J Perinatol 2021 Jun 30. Epub 2021 Jun 30.

Department of Neonatology, Affiliated Hospital of Southwest Medical University, Luzhou, China.

Objective:  The study aimed to investigate the effects of kangaroo mother care (KMC) on repeated procedural pain and cerebral oxygenation in preterm infants.

Study Design:  Preterm infants of 31 to 33 weeks of gestational age were randomly divided into an intervention group ( = 36) and a control group ( = 37). Premature infant pain profile (PIPP) scores, heart rate, oxygen saturation, regional cerebral tissue oxygenation saturation (rcSO), and cerebral fractional tissue oxygen extraction (cFTOE) were evaluated during repeated heel stick procedures. Each heel stick procedure included three phases: baseline, blood collection, and recovery. KMC was given to the intervention group 30 minutes before baseline until the end of the recovery phase.

Results:  Compared with the control group, the intervention group showed lower PIPP scores and heart rates, higher oxygen saturation, and rcSO from the blood collection to recovery phases during repeated heel sticks. Moreover, there were significant changes in cFTOE for the control group, but not the intervention group associated with repeated heel stick procedures.

Conclusion:  The analgesic effect of KMC is sustained over repeated painful procedures in preterm infants, and it is conducive to stabilizing cerebral oxygenation, which may protect the development of brain function.

Key Points: · KMC stabilizes cerebral oxygenation during repeated heel sticks in preterm infants.. · The analgesic effect of KMC is sustained over repeated painful procedures in preterm infants.. · KMC may protect the development of brain function..
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http://dx.doi.org/10.1055/s-0041-1731650DOI Listing
June 2021

Effect of Acupuncture on Neuroplasticity of Stroke Patients with Motor Dysfunction: A Meta-Analysis of fMRI Studies.

Neural Plast 2021 2;2021:8841720. Epub 2021 Jun 2.

Department of Neurology and Stroke Center, Dongzhimen Hospital, The First Affiliated Hospital of Beijing University of Chinese Medicine, Beijing, China.

Objective: To analyze the pattern of intrinsic brain activity variability that is altered by acupuncture compared with conventional treatment in stroke patients with motor dysfunction, thus providing the mechanism of stroke treatment by acupuncture.

Methods: Chinese and English articles published up to May 2020 were searched in the PubMed, Web of Science, EMBASE, and Cochrane Library databases, China National Knowledge Infrastructure, Chongqing VIP, and Wanfang Database. We only included randomized controlled trials (RCTs) using resting-state fMRI to observe the effect of acupuncture on stroke patients with motor dysfunction. R software was used to analyze the continuous variables, and Seed-based d Mapping with Permutation of Subject Images (SDM-PSI) was used to perform an analysis of fMRI data. . A total of 7 studies comprising 143 patients in the treatment group and 138 in the control group were included in the meta-analysis. The results suggest that acupuncture treatment helps the healing process of motor dysfunction in stroke patients and exhibits hyperactivation in the bilateral basal ganglia and insula and hypoactivation in motor-related areas (especially bilateral BA6 and left BA4).

Conclusion: Acupuncture plays a role in promoting neuroplasticity in subcortical regions that are commonly affected by stroke and cortical motor areas that may compensate for motor deficits, which may provide a possible mechanism underlying the therapeutic effect of acupuncture.
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http://dx.doi.org/10.1155/2021/8841720DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8192216PMC
June 2021

Dysregulated metabolism and behaviors by disrupting gut microbiota in prenatal and neonatal mice.

Anim Sci J 2021 Jan;92(1):e13566

Laboratory of Regulation in Metabolism and Behavior, Department of Bioresource Sciences, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan.

The live microbiota ecosystem in the intestine plays a critical role in maintaining the normal physiological and psychological functions in both animals and human beings. However, the chronic effect of microbiota disturbances during prenatal and neonatal developing periods on animal's health remains less studied. In the current study, pregnant ICR mice were fed with an antibiotic diet (7-g nebacitin [bacitracin-neomycin sulphate 2:1]/kg standard diet) from day 14 of conception, and their offspring were provided with the same diet till the termination of the experiments. Dams treated with antibiotics showed increased body weight along with enlarged gut. Antibiotic-treated offspring revealed decreased bodyweight, increased food, water, and sucrose intake. Administration of antibiotics affected corticosterone responsivity to acute 20 min restraint challenge in male pups. In behavior tests, female pups showed decreased movement in open field while male pups revealed decreased latency to open arms in elevated plus maze test and immobility time in tail suspension test. Together, these results suggested that early antibiotic exposure may impact on the food intake, body weight gain, and emotional behavior regulation in mice.
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http://dx.doi.org/10.1111/asj.13566DOI Listing
January 2021

NADPH is superior to NADH or edaravone in ameliorating metabolic disturbance and brain injury in ischemic stroke.

Acta Pharmacol Sin 2021 Jun 24. Epub 2021 Jun 24.

Department of Pharmacology and Laboratory of Aging and Nervous Diseases, Jiangsu Key Laboratory of Neuropsychiatric Diseases, College of Pharmaceutical Sciences, Soochow University, Suzhou, 215123, China.

Our previous studies confirm that exogenous reduced nicotinamide adenine dinucleotide phosphate (NADPH) exerts a neuroprotective effect in animal models of ischemic stroke, and its primary mechanism is related to anti-oxidative stress and improved energy metabolism. However, it is unknown whether nicotinamide adenine dinucleotide (NADH) also plays a neuroprotective role and whether NADPH is superior to NADH against ischemic stroke? In this study we compared the efficacy of NADH, NADPH, and edaravone in ameliorating brain injury and metabolic stress in ischemic stroke. Transient middle cerebral artery occlusion/reperfusion (t-MCAO/R) mouse model and in vitro oxygen glucose deprivation/reoxygenation (OGD/R) model were established. The mice were intravenously administered the optimal dose of NADPH (7.5 mg/kg), NADH (22.5 mg/kg), or edaravone (3 mg/kg) immediately after reperfusion. We showed that the overall efficacy of NADPH in ameliorating ischemic injury was superior to NADH and edaravone. NADPH had a longer therapeutic time window (within 5 h) after reperfusion than NADH and edaravone (within 2 h) for ischemic stroke. In addition, NADPH and edaravone were better in alleviating the brain atrophy, while NADH and NADPH were better in increasing the long-term survival rate. NADPH showed stronger antioxidant effects than NADH and edaravone; but NADH was the best in terms of maintaining energy metabolism. Taken together, this study demonstrates that NADPH exerts better neuroprotective effects against ischemic stroke than NADH and edaravone.
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http://dx.doi.org/10.1038/s41401-021-00705-5DOI Listing
June 2021

A zwitterionic polypeptide nanocomposite with unique NIR-I/II photoacoustic imaging for NIR-I/II cancer photothermal therapy.

J Mater Chem B 2021 Jul;9(27):5484-5491

Joint Research Center for Precision Medicine, Department of Obstetrics and Gynecology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital South Campus, Shanghai Fengxian Central Hospital, Shanghai 201499, P. R. China.

The second near infrared photoacoustic imaging (NIR-II PAI) and photothermal therapy (NIR-II PTT) have attracted wide interest in cancer theranostics because of maximum permission exposure (MPE), deep penetration, and lower scattering and background noise compared to NIR-I counterparts; however, it is imperative to develop biocompatible nanomaterials having NIR-II response. By utilizing multivalent Au-S coordination bonds, we constructed a zwitterionic polypeptide nanocomposite of [email protected] with a suitable size of 48 ± 2 nm, which possessed a strong and broad absorbance at 650-1100 nm and an excellent photothermal conversion efficiency of 49.5%. In vitro biological studies demonstrated that NIR-II PTT within MPE was more effective than NIR-I PTT beyond MPE. Along with X-ray computed tomography and photothermal imaging functions, [email protected] in vivo presented unique NIR-I/II PAI with 2.6-5.9 times signal enhancement compared to the contrast. By single dose and NIR-II irradiation (1064 nm, 1 W cm-2, 10 min), NIR-II PTT within MPE completely eradicated MCF-7 tumors without tissue damage and tumor recurrence within 24 days, inducing a better antitumor efficacy than NIR-I PTT beyond MPE. Importantly, this study provides an innovative method for the fabrication of biocompatible zwitterionic polypeptide nanocomposites with unique NIR-I/II PAI and NIR-II PTT attributes, thus holding great potential for precise cancer theranostics and further clinical transitions.
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http://dx.doi.org/10.1039/d1tb00823dDOI Listing
July 2021

Comparison of the Metabolic Profiles in the Plasma and Urine Samples Between Autistic and Typically Developing Boys: A Preliminary Study.

Front Psychiatry 2021 4;12:657105. Epub 2021 Jun 4.

Key Laboratory for Neuroscience, Ministry of Education of China, Neuroscience Research Institute, Beijing, China.

Autism spectrum disorder (ASD) is defined as a pervasive developmental disorder which is caused by genetic and environmental risk factors. Besides the core behavioral symptoms, accumulated results indicate children with ASD also share some metabolic abnormalities. To analyze the comprehensive metabolic profiles in both of the first-morning urine and plasma samples collected from the same cohort of autistic boys. In this study, 30 autistic boys and 30 tightly matched healthy control (HC) boys (age range: 2.4~6.7 years) were recruited. First-morning urine and plasma samples were collected and the liquid chromatography-mass spectrometry (LC-MS) was applied to obtain the untargeted metabolic profiles. The acquired data were processed by multivariate analysis and the screened metabolites were grouped by metabolic pathway. Different discriminating metabolites were found in plasma and urine samples. Notably, taurine and catechol levels were decreased in urine but increased in plasma in the same cohort of ASD children. Enriched pathway analysis revealed that perturbations in taurine and hypotaurine metabolism, phenylalanine metabolism, and arginine and proline metabolism could be found in both of the plasma and urine samples. These preliminary results suggest that a series of common metabolic perturbations exist in children with ASD, and confirmed the importance to have a comprehensive analysis of the metabolites in different biological samples to reveal the full picture of the complex metabolic patterns associated with ASD. Further targeted analyses are needed to validate these results in a larger cohort.
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http://dx.doi.org/10.3389/fpsyt.2021.657105DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8211775PMC
June 2021
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