Publications by authors named "Rong Zeng"

621 Publications

Effect Evaluation of Preoperative Psychological Nursing Intervention on Sinusitis Patients Undergoing General Anesthesia by Multiplanner Reformation-Based CT.

Comput Math Methods Med 2022 28;2022:7516339. Epub 2022 Jul 28.

Day Operating Room, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Guangzhou, 510000 Guangdong, China.

The aim of this study was at exploring the clinical effect of CT images based on multiplanner reformation (MPR) combined with a preoperative psychological nursing intervention model in sinusitis patients undergoing general anesthesia. Sixty sinusitis patients who received MPR-based CT examination and general anesthesia were selected as the study subjects and randomly divided into the control group ( = 30) and the experimental group ( = 30). The control group used traditional preoperative education. The experimental group added the psychological nursing intervention based on traditional preoperative education. The blood pressure and heart rate before and after the operation, the self-rating anxiety scale (SAS) score before and after intervention, and satisfaction were comprehensively assessed. The results showed that CT based on MPR could observe the lesions and anatomical structures of the sinus wall and sinus in detail from multiple angles. The blood pressure (systolic blood pressure 135.12 ± 14.89 mmHg, diastolic blood pressure 87.05 ± 11.24 mmHg), heart rate (78.42 ± 12.19 beats/min), SAS score (45.85 ± 4.97 points), and nursing satisfaction (78.9%) of the experimental group were significantly better than those of the control group (145.83 ± 15.62 mmHg, 94.21 ± 10.86 mmHg, 86.44 ± 13.65 beats/min, 56.44 ± 5.12 points, 56.4%), and the differences were statistically significant ( < 0.05). In summary, the preoperative psychological care model has a positive role in reducing the tension and anxiety of patients before general anesthesia surgery and CT based on MPR is important for the clinical diagnosis and treatment of sinusitis. This study provides a theoretical reference for the clinical treatment of patients with sinusitis.
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http://dx.doi.org/10.1155/2022/7516339DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9352500PMC
July 2022

Effects and safety of metformin in patients with concurrent diabetes mellitus and chronic obstructive pulmonary disease: a systematic review and meta-analysis.

Endocr Connect 2022 Jul 1. Epub 2022 Jul 1.

L Dong, Shandong Qianfoshan Hospital, Ji-nan, 250014, China.

Aim: This study aimed to investigate the effects and safety of metformin in patients with concurrent diabetes mellitus (DM) and chronic obstructive pulmonary disease (COPD).

Methods: PubMed, Embase, Web of Science, the China National Knowledge, and Cochrane Database were searched to find studies that examined the effects and safety of metformin in patients with concurrent DM and COPD. We conducted a meta-analysis with a risk ratio (RR) and assessed the quality of included studies and pooled evidence.

Results: Eight articles were involved. Metformin decreased the risk of COPD-related hospitalizations (RR 0.72, 95% CI 0.53-0.98; I2=89%) and all-cause mortality (RR 0.60, 95% CI 0.36-1.01, I2=69%) in patients with concurrent DM and COPD, but didn't increase the risk of hyperlactatemia (RR 1.14, 95%CI 0.92-1.41, I2 =8%).

Conclusions: Metformin use in patients with concurrent DM and COPD might lower COPD-related hospitalizations and the risk of all-cause mortality without increasing the risk of hyperlactatemia. Considerations should be given to conduct more high-quality randomized trials involving larger samples.
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http://dx.doi.org/10.1530/EC-22-0289DOI Listing
July 2022

Analysis of Revision Surgery Following Surgical Reconstruction of the Sigmoid Sinus Wall in Patients with Pulsatile Tinnitus.

J Int Adv Otol 2022 Jul;18(4):358-364

Department of Otolaryngology Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

Background: To explore potential causes leading to the failure of the surgical reconstruction of sigmoid sinus wall in patients with pulsatile tinnitus.

Methods: This study retrospectively analyzed pulsatile tinnitus patients with sigmoid sinus wall dehiscence and/or sigmoid sinus diverticulum, who underwent revision surgeries following the surgical reconstruction of the sigmoid sinus wall.

Results: A total of 7 pulsatile tinnitus patients were included. After the first surgery, 5 of 7 patients showed varying degrees of remission in pulsatile tinnitus, but pulsatile tinnitus gradually recurred. The other 2 patients experienced no change in pulsatile tinnitus. Images after the first surgeries revealed that the surgical failure of Patients 1-5 was caused by incompleted elimination of sigmoid sinus wall dehiscence and sigmoid sinus diverticulum. Following revision surgeries, they all acquired great or complete resolution of pulsatile tinnitus. In Patient 6, an abnormal diploic vein entering the sigmoid sinus was overlooked during the first surgery for repairing sigmoid sinus wall dehiscence. As soon as the diploic vein was blocked during the revision surgery, the patient's pulsatile tinnitus was alleviated significantly. Patient 7 had a recurrence of pulsatile tinnitus after transient improvement following the first surgery, and still experienced no change in pulsatile tinnitus after the revision surgery. However, after venous sinus stenting in the stenosis of transverse-sigmoid sinus junction, she got complete resolution of pulsatile tinnitus. It sug- gested that the unresolved stenosis of the venous sinus was the cause of the surgical failure.

Conclusions: Both sigmoid sinus diverticulum and sigmoid sinus wall dehiscence are recommended to be resolved during surgery. Abnormal diploic veins and venous sinus stenosis are potential causes of pulsatile tinnitus.
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http://dx.doi.org/10.5152/iao.2022.21471DOI Listing
July 2022

An Intratumor Heterogeneity-Related Signature for Predicting Prognosis, Immune Landscape, and Chemotherapy Response in Colon Adenocarcinoma.

Front Med (Lausanne) 2022 7;9:925661. Epub 2022 Jul 7.

Department of Gastroenterology, Digestive Disease Hospital, The First Affiliated Hospital of Nanchang University, Nanchang, China.

Background: Colon adenocarcinoma (COAD) is a frequent malignancy of the digestive system with a poor prognosis and high mortality rate worldwide. Intratumor heterogeneity (ITH) is associated with tumor progression, poor prognosis, immunosuppression, and therapy resistance. However, the relationship between ITH and prognosis, the immune microenvironment, and the chemotherapy response in COAD patients remains unknown, and this knowledge is urgently needed.

Methods: We obtained clinical information and gene expression data for COAD patients from The Cancer Genome Atlas (TCGA) database. The DEPTH2 algorithm was utilized to evaluate the ITH score. X-tile software was used to determine the optimal cutoff value of the ITH score. The COAD patients were divided into high- and low-ITH groups based on the cutoff value. We analyzed prognosis, tumor mutation burden (TMB), gene mutations, and immune checkpoint expression between the high- and low-ITH groups. Differentially expressed genes (DEGs) in the high- and low-ITH groups were subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses. We performed univariate Cox regression and least absolute shrinkage and selection operator (LASSO) regression analyses to screen the prognosis-related genes for the construction of an ITH-related prognostic signature. The nomogram was used to predict the overall survival (OS) of COAD patients. The protein-protein interaction (PPI) network was constructed by using the GeneMANIA database. Principal component analysis (PCA) and single-sample gene set enrichment analysis (ssGSEA) were employed to explore the differences in biological pathway activation status between the high- and low-risk groups. The proportion and type of tumor-infiltrating immune cells were evaluated by the CIBERSORT and ESTIMATE algorithms. Additionally, we assessed the chemotherapy response and predicted small-molecule drugs for treatment. Finally, the expression of the prognosis-related genes was validated by using the UALCAN database and Human Protein Atlas (HPA) database.

Results: The OS of the high-ITH group was worse than that of the low-ITH group. A positive correlation between ITH and TMB was identified. In subgroups stratified by age, gender, and tumor stage, the OS of the low-ITH group remained better than that of the high-ITH group. There were dramatic differences in the mutated genes, single nucleotide variant classes, variant types, immune checkpoints and cooccurring and mutually exclusive mutations of the DEGs between the high- and low-ITH groups. Based on the DEGs between the high- and low-ITH groups, we constructed a five-gene signature consisting of CEACAM5, ENO2, GABBR1, MC1R, and SLC44A4. The COAD patients were divided into high- and low-risk groups according to the median risk score. The OS of the high-risk group was worse than that of the low-risk group. The nomogram was used to accurately predict the 1-, 3- and 5-year OS of COAD patients and showed good calibration and moderate discrimination ability. The stromal score, immune score, and ESTIMATE score of the high-risk group were significantly higher than those of the low-risk group, whereas tumor purity showed the opposite trend. The patients classified by the risk score had distinguishable sensitivity to chemotherapeutic drugs. Finally, two public databases confirmed that CEACAM5 and SLC44A4 were upregulated in normal tissues compared with COAD tissues, and ENO2, GABBR1, and MC1R were upregulated in COAD tissues compared with normal tissues.

Conclusion: Overall, we identified an ITH-related prognostic signature for COAD that was closely related to the tumor microenvironment and chemotherapy response. This signature may help clinicians make more personalized and precise treatment decisions for COAD patients.
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http://dx.doi.org/10.3389/fmed.2022.925661DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302538PMC
July 2022

Endoplasmic Reticulum Stress Contributes to Ventilator-Induced Diaphragm Atrophy and Weakness in Rats.

Front Physiol 2022 27;13:897559. Epub 2022 Jun 27.

Emergency Center, Hubei Clinical Research Center for Emergency and Resuscitation, Zhongnan Hospital of Wuhan University, Wuhan, China.

Accumulating evidence indicates that endoplasmic reticulum (ER) stress plays a critical role in the regulation of skeletal muscle mass. In recent years, much attention has been given to ventilator-induced diaphragm dysfunction (VIDD) because it strongly impacts the outcomes of critically ill patients. Current evidence suggests that the enhancement of oxidative stress is essential for the development of VIDD, but there are no data on the effects of ER stress on this pathological process. VIDD was induced by volume-controlled mechanical ventilation (MV) for 12 h; Spontaneous breathing (SB, for 12 h) rats were used as controls. The ER stress inhibitor 4-phenylbutyrate (4-PBA), the antioxidant N-acetylcysteine (NAC), and the ER stress inducer tunicamycin (TUN) were given before the onset of MV or SB. Diaphragm function, oxidative stress, and ER stress in the diaphragms were measured at the end of the experiments. ER stress was markedly increased in diaphragms relative to that in SB after 12 h of MV (all < 0.001). Inhibition of ER stress by 4-PBA downregulated the expression levels of proteolysis-related genes in skeletal muscle, including -1 and -1, reduced myofiber atrophy, and improved diaphragm force-generating capacity in rats subjected to MV (all < 0.01). In addition, mitochondrial reactive oxygen species (ROS) production and protein level of 4-HNE (4-hydroxynonenal) were decreased upon 4-PBA treatment in rats during MV (all < 0.01). Interestingly, the 4-PBA treatment also markedly increased the expression of peroxisome proliferator-activated receptor-gamma co-activator-1alpha (PGC-1α) ( < 0.01), a master regulator for mitochondrial function and a strong antioxidant. However, the antioxidant NAC failed to reduce ER stress in the diaphragm during MV ( 0.05). Finally, ER stress inducer TUN largely compromised diaphragm dysfunction in the absence of oxidative stress (all < 0.01). ER stress is induced by MV and the inhibition of ER stress alleviates oxidative stress in the diaphragm during MV. In addition, ER stress is responsible for diaphragm dysfunction in the absence of oxidative stress. Therefore, the inhibition of ER stress may be another promising therapeutic approach for the treatment of VIDD.
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http://dx.doi.org/10.3389/fphys.2022.897559DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9273093PMC
June 2022

γ-Polyglutamic Acid Production, Biocontrol, and Stress Tolerance: Multifunction of A-5 and the Complete Genome Analysis.

Int J Environ Res Public Health 2022 Jun 22;19(13). Epub 2022 Jun 22.

Eco-Environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.

A-5 has the capabilities of high-molecular-weight γ-PGA production, antagonism to plant pathogenic fungi, and salt/alkaline tolerance. This multifunctional bacterium has great potential for enhancing soil fertility and plant security in agricultural ecosystem. The genome size of A-5 was 4,190,775 bp, containing 1 Chr and 2 plasmids (pA and pB) with 43.37% guanine-cytosine content and 4605 coding sequences. The γ-PGA synthase gene cluster was predicted to consist of and factor (). The γ-PGA-degrading enzymes were mainly pgdS, GGT, and cwlO. Nine gene clusters producing secondary metabolite substances, namely, four unknown function gene clusters and five antibiotic synthesis gene clusters (surfactin, fengycin, bacillibactin, subtilosin_A, and bacilysin), were predicted in the genome of A-5 using antiSMASH. In addition, A-5 contained genes related to carbohydrate and protein decomposition, proline synthesis, pyruvate kinase, and stress-resistant proteins. This affords significant insights into the survival and application of A-5 in adverse agricultural environmental conditions.
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http://dx.doi.org/10.3390/ijerph19137630DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9265942PMC
June 2022

Downregulated Copper Homeostasis-Related Gene FOXO1 as a Novel Indicator for the Prognosis and Immune Response of Breast Cancer.

J Immunol Res 2022 28;2022:9140461. Epub 2022 Jun 28.

Xiangya International Medical Center, Xiangya Hospital, Central South University, Changsha 410008, China.

Copper (Cu) is one of the essential microelements for all living systems. Studies have illustrated the biological significance of Cu homeostasis in human cancers, including breast cancer (BRCA). Nevertheless, the detailed roles of Cu homeostasis in BRCA need to be further explored. Here, we identified a downregulated Cu homeostasis-related gene FOXO1 and investigated the potential functions of FOXO1 in BRCA through several bioinformation databases. The BRCA patients with high level of FOXO1 displayed favorable prognostic values. Subsequently, enrichment analysis of FOXO1 coexpressed genes revealed that the top three enriched KEGG pathways were spliceosome, oxidative phosphorylation, and ribosome. Immunoinfiltration analysis indicated that aberrantly expressed FOXO1 showed positive correlations with the subcellular infiltration of macrophages and neutrophils in BRCA. Moreover, FOXO1 expression was positively associated with multiple immune checkpoints, such as sialic acid-binding immunoglobulin-like lectin 15 (SIGLEC15), indoleamine 2,3-dioxygenase 1 (IDO1), programmed cell death 1 ligand 1 (PD-L1/CD274), hepatitis A virus cellular receptor 2 (HAVCR2), programmed cell death 1 (PDCD1), cytotoxic T lymphocyte antigen 4 (CTLA4), and programmed cell death 1 ligand 2 (PDCD1LG2). Overall, these findings would deepen our understanding of FOXO1 in BRCA prognosis and immunotherapy response, representing a promising therapeutic strategy for BRCA patients.
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http://dx.doi.org/10.1155/2022/9140461DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9256448PMC
July 2022

Blood Pressure Control and Renal Function Preservation of ex vivo Renal Artery Repair with Orthotopic Renal Autotransplantation for Complex Renal Artery Diseases.

J Vasc Surg 2022 Jul 4. Epub 2022 Jul 4.

Department of Vascular Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, PR China. Electronic address:

Objective: To assess the outcome of ex vivo renal artery repair with orthotopic renal autotransplantation for complex renal artery disease patients.

Methods: The single-center study collected and analyzed the data from complex renal artery disease patients undergoing ex vivo renal artery repair with orthotopic renal autotransplantation, retrospectively.

Results: A total of 21 complex renal artery lesions from 19 patients were included. The mean blood pressure showed a significant decrease from the preoperative to the postoperative period (p<.05). Renal function kept stable for the perioperative period. No significant serum creatinine & estimated glomerular filtration rate alteration was observed compared with immediate postoperative period (p=.439 and .904, respectively). The median renal cold ischemia time was 35.5 (76) min. Two patients developed perioperative complications, one with acute cholecystitis and one with acute renal failure after graft occlusion in solitary kidney. During the median follow-up of 48 months, one single bypass graft of a solitary kidney was occluded, and four grafts developed restenosis. The primary, and primary-assisted patency rates at the 5-year follow-up were 81.3%, and 87.5%, respectively. No deaths were observed in the follow-up period.

Conclusions: Ex vivo renal artery reconstruction with orthotopic renal autotransplantation in complex renal artery disease patients offer stable control of blood pressure and renal function preservation, and should be considered as a potential alternative for other open surgical procedures.
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http://dx.doi.org/10.1016/j.jvs.2022.04.057DOI Listing
July 2022

Mild Amide Synthesis Using Nitrobenzene under Neutral Conditions.

Org Lett 2022 Jul 27;24(26):4766-4771. Epub 2022 Jun 27.

School of Chemistry, Xi'an Jiaotong University, Xi'an 710049, P. R. China.

Amide synthesis is one of the most important transformations in organic chemistry due to the broad application in pharmaceutical drugs and organic materials. In this report, we describe a mild protocol for amide formation using the readily available nitroarenes as nitrogen sources and an inexpensive iron complex as a catalyst. Because of the use of the pH-neutral conditions and the avoidance of the strong oxidant or reductant, a wide range of aromatic and aliphatic aldehydes as well as nitroarenes with various functional groups could be tolerated well. A plausible mechanism is proposed based on the detailed studies, in which iron catalyst initiates the radical process and the solvent plays a key role as O-atom acceptor.
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http://dx.doi.org/10.1021/acs.orglett.2c01743DOI Listing
July 2022

Multi-responsive mesoporous polydopamine composite nanorods cooperate with nano-enzyme and photosensitiser for intensive immunotherapy of bladder cancer.

Immunology 2022 Jun 25. Epub 2022 Jun 25.

Shengli Clinical Medical College of Fuiian Medical University, Fuzhou, China.

Bladder cancer is a common malignancy in the urinary system. Defects of drug molecules in bladder during treatment, such as passive diffusion, rapid clearance of periodic urination, poor adhesion and permeation abilities, lead to low delivery efficiency of conventional drugs and high recurrence rate of disease. In this study, we designed multi-responsive mesoporous polydopamine (PDA) composite nanorods cooperating with nano-enzyme and photosensitiser for intensive immunotherapy of bladder cancer. The strongly adhesive mesoporous PDA with wheat germ agglutinin on nanoparticles could specifically adhere to epithelial glycocalyx and made the nanoparticles aggregate in urinary pathways. Meanwhile, 2,3-dimethylmaleic anhydride could be hydrolysed in acidic conditions of tumour microenvironment, giving it a positive charge (charge reversal), which is more amenable to enter cancer cells. Afterwards, manganese dioxide nanorods could catalyse the reaction of excess H O in tumour microenvironment to generate active oxygen, so as to change the hypoxic environment in tumour, and achieve a pH-responsive for slow release of PD-L1. After the ICG was irradiated by infrared light, a large amount of singlet oxygen was generated, thereby enhancing the therapeutic effect and reducing toxicity in vivo. Besides, mesoporous PDA with indocyanine green photothermal agent could have a local heat up quickly under the near-infrared light to kill cancer cells, thereby enhancing therapeutic efficacy. Accordingly, this mesoporous PDA composite nanorods shed a light on bladder tumour treatment.
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http://dx.doi.org/10.1111/imm.13534DOI Listing
June 2022

The Interferon Gamma-Related Long Noncoding RNA Signature Predicts Prognosis and Indicates Immune Microenvironment Infiltration in Colon Adenocarcinoma.

Front Oncol 2022 7;12:876660. Epub 2022 Jun 7.

Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang, China.

Colon adenocarcinoma (COAD) is one of the most common clinically malignant tumours of the digestive system, with high incidence and mortality and poor prognosis. Interferon-gamma (IFN-γ) and long noncoding RNAs (lncRNAs) have prognostic values and were closely associated with immune microenvironment in COAD. Thus, identifying IFN-γ-related lncRNAs may be valuable in predicting the survival of patients with COAD. In this study, we identified IFN-γ-related lncRNAs and divided COAD patients from the Cancer Genome Atlas (TCGA) database into training and validation sets. Pearson's correlation analysis and least absolute shrinkage and selection operator (LASSO) Cox regression were performed to select IFN-γ-related lncRNA-associated prognoses. Thirteen lncRNAs (AC025165.8, AC091633.3, FENDRR, LINC00882, LINC01828, LINC01829, MYOSLID, RP11-154H23.4, RP11-20J15.3, RP11-324L17.1, RP11-342A23.2, RP11-805I24.3, SERTAD4-AS1) were identified to construct an IFN-γ-related lncRNA prognostic signature in TCGA training (n =213) and validation (n =213) cohorts. COAD patient risk scores were calculated and classified into high- and low-risk groups based on the median value of the risk scores in each dataset. We compared the overall survival (OS) of patients stratified by age, gender, and stage. The OS in the high-risk group was significantly shorter than that in the low-risk group. In addition, the clinical nomogram incorporating the prognostic signature and clinical features showed a high concordance index of 0.78 and accurately predicted 1-, 3-, and 5-year survival times among COAD patients in the high- and low-risk groups. Based on the risk model, the high- and low-risk groups exhibited distinct differences in the immune system by gene set enrichment analysis (GSEA) functional annotation, and differentially expressed genes (DEGs) between the high- and low-risk groups were subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. We investigated the expression of multiple immune checkpoint genes in the high- and low-risk groups and plotted Kaplan-Meier survival curves, indicating that immune checkpoint genes, such as LAG3 and PD. L1, STING and TIM 3, were also expressed differently between the two risk groups. Subsequently, there were dramatic differences in mutated genes, SNV (single nucleotide variants) classes, variant types and variant allele frequencies between low- and high-risk patients with COAD. Patients stratified by risk scores had different sensitivities to common chemotherapeutic agents. Finally, we used quantitative real-time polymerase chain reaction (qRT-PCR) assays to demonstrate that three lncRNAs were significantly differentially expressed in COAD tissues and adjacent normal tissues. Considered together, a thirteen-lncRNA prognostic signature has great potential to be a prognostic biomarker and could play an essential role in the immune microenvironment of COAD.
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http://dx.doi.org/10.3389/fonc.2022.876660DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9211770PMC
June 2022

The Effect of Humanized Nursing Intervention Guided by Computed Tomography Images on Elderly Patients Undergoing Anesthesia for Femur Intertrochanteric Fractures under Intelligent Reconstruction Algorithm.

Comput Math Methods Med 2022 24;2022:5070518. Epub 2022 May 24.

Department of Anesthesia Surgery Department, Changsha Fourth Hospital, Changsha, 410006 Hunan, China.

This research was aimed at analyzing the effect of humanized nursing intervention combined with computed tomography (CT) imaging in the surgical anesthesia of femur intertrochanteric fractures (FIF) in the elderly. An image reconstruction algorithm was proposed based on nonlocal mean (NLM) algorithm, which was named as ONLM, and its performance was analyzed. A total of 114 elderly patients with FIF were equally and randomly divided into a humanized nursing group (57 cases) and a routine nursing group (57 cases). They were performed with CT imaging scan based on the ONLM algorithm, and the clinical indicators of the two groups of patients were recorded. The root mean square error (RMSE) and mean absolute error (MAE) of the CT images constructed using the ONLM algorithm were significantly lower than those using NLM algorithm, edge filtering algorithm, and total variation model, while the peak signal-to-noise ratio (PSNR) was the opposite ( < 0.05). The operation time, hospitalization days, intraoperative blood loss, postoperative drainage, and anesthesia preparation time of patients in the humanized nursing group were significantly lower than those in the routine nursing group. The number of patients with excellent Harris scores in the humanized nursing group was higher than that in the routine nursing group, and the number of patients with poor Harris scores was lower ( < 0.05). The language pain score, facial pain score, and visual analog simulation (VAS) scores of patients in the humanized nursing group were significantly lower than those in the routine nursing group. The numbers of postoperative hip varus and fracture nonunion cases in the humanized nursing group were significantly more than those in the routine nursing group. In short, CT images constructed by the ONLM showed higher performance than those by the traditional algorithm. In addition, CT images constructed by ONLM combined with humanized nursing intervention could more effectively improve the cooperation of patients with surgical anesthesia, reduce surgical pain and fear of patients, improve the prognosis of patients, and lower the occurrence of adverse events.
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http://dx.doi.org/10.1155/2022/5070518DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9155936PMC
June 2022

No significant relationship exists between tumor size and prognosis in distant metastatic hepatocellular carcinoma: a propensity score matching analysis based on SEER database.

BMC Gastroenterol 2022 Jun 2;22(1):274. Epub 2022 Jun 2.

Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, No. 17 Yongwaizheng Street, Nanchang, Jiangxi Province, China.

Background: Previous studies have shown that tumor size has an impact on the prognosis of hepatocellular carcinoma (HCC). Whether tumor size is related to the prognosis of distant metastatic HCC is unclear. The purpose of this study was to investigate the effect of tumor size on the prognosis of distant metastatic HCC.

Methods: Data on patients with HCC were collected from the (SEER) database of surveillance, epidemiology and final results. Propensity score matching (PSM) was used to reduce confounding factors and comprehensively evaluate the clinicopathological features and prognosis of distant metastatic HCC.

Results: There were 189 patients with distant metastatic HCC whose tumor size was ≤ 50 mm and 615 patients with a tumor size > 50 mm. The tumor sizes of distant metastatic HCC patients were associated with race, grade, surgical treatment, N and AFP. The Kaplan-Meier analysis showed that the mortality rate of patients with a tumor size > 50 mm was higher than that of patients with a tumor size ≤ 50 mm (p = 0.00062). However, there were no significant differences in mortality rates after adjusting for confounding variables by using propensity score matching (p = 0.23).

Conclusion: This propensity score matching study provides the best data in support of the following assertions: tumor size is not an independent prognostic factor for distant metastatic HCC.
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http://dx.doi.org/10.1186/s12876-022-02355-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9161599PMC
June 2022

Analgesic Effects of Dexmedetomidine Combined with Spinal and Epidural Anesthesia Nursing on Prostate Hyperplasia Patients after Transurethral Resection of Prostate by Intelligent Algorithm-Based Magnetic Resonance Imaging.

Comput Math Methods Med 2022 21;2022:4243244. Epub 2022 May 21.

Department of Anesthesia Surgery, Changsha Fourth Hospital, Changsha, 410006 Hunan, China.

To analyze the investigation of the application effects of different doses of dexmedetomidine (Dex) with combined spinal and epidural anesthesia nursing on analgesia after transurethral resection of prostate (TURP) by intelligent algorithm-based magnetic resonance imaging (MRI), MRI imaging segmentation model of mask regions with convolutional neural network (Mask R-CNN) features was proposed in the research. Besides, the segmentation effects of Mask R-CNN, U-net, and V-net algorithms were compared and analyzed. Meanwhile, a total of 184 patients receiving TURP were selected as the research objects, and they were divided into A, B, C, and D groups based on random number table method, each group including 46 cases. Patients in each group were offered different doses of Dex, and visual analogue scale (VAS) and Ramsay scores of different follow-up visit time, use of other analgesics, the incidence of postoperative cystospasm, and nursing satisfaction of patients in four groups were compared. The results demonstrated that Dice similarity coefficient (DSC) value, specificity, and positive predictive value of Mask R-CNN algorithm were 0.623 ± 0.084, 98.61%, and 69.57%, respectively, all of which were higher than those of U-net and V-net algorithms. Pain VAS scores and the incidence of cystospasm at different time periods of groups B and C were both significantly lower than those of group D ( < 0.05). Ramsay scores of groups B and C at 8 hours, 12 hours, 24 hours, and 48 hours after the operation were all remarkably higher than those in group D ( < 0.05). Besides, nursing satisfaction of groups B and C was obviously superior to that in group D, and the difference demonstrated statistical meaning ( < 0.05). The differences revealed that Dex showed excellent analgesic and sedative effects and could effectively reduce the incidence of complications after TURP, including cystospasm and nausea. In addition, it helped improve nursing satisfaction and patient prognosis.
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http://dx.doi.org/10.1155/2022/4243244DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9148224PMC
June 2022

First Report of Pectobacterium brasiliense causing Soft Rot on Conophytum luiseae in China.

Plant Dis 2022 May 25. Epub 2022 May 25.

Shanghai Academy of Agricultural Sciences, 74594, Eco-Environmental Protection Research Institute, Shanghai, China;

Conophytum luiseae is native to the Namaqualand region of Cape, South Africa. It is a lovely plant with many short succulent spines on ingot-shaped fleshy leaf surfaces, and a high-value ornamental plant in China. In August to October 2021, a serious soft rot disease on Conophytum luiseae plants was observed in four greenhouses at a horticultural farm in Songjiang District, Shanghai, China. 70% of Conophytum luiseae plants on this farm had severe rot symptoms. Initially, wilting and soft rot symptoms appeared on fleshy leaves, then progressed into browning and withering symptoms of all fleshy leaves. To isolate and identify the causal agent, small pieces of lesion tissues were sterilized by 75% ethanol for 30 s, and rinsed three times with sterile water. Later, the tissues were crushed in sterile 2.0 mL centrifuge tube with 100 μl of sterile water. The suspension was serially diluted and spread on Luria-Bertani agar (LB) medium. After incubation at 28°C for 48 h, the bacterial colonies were tiny and streaked on LB plate for purification. After purification, five independent representative colonies were used for further confirmation. Genomic DNA from the bacterial isolate was extracted and used as the template to amplify 16s rDNA with primers 27F/1492R (Ying et al. 2012) and the housekeeping genes, dnaX with primers dnaXF/ dnaXR (Slawiak et al. 2009), and leuS with primers leuSF/ leuSR (Portier et al. 2019), respectively, by a polymerase chain reaction (PCR). The 16S rRNA sequences of one bacterial isolate was deposited in GenBank (GenBank accession OM333246) and showed a 99% similarity to that of Pectobacterium brasiliense (syn. Pectobacterium carotovorum subsp. brasiliense, Pcb) strains HG1501090309 (KU997683), BC1(CP009769), KC08 (KY021029). The dnaX (OM320998) and leuS (OM321306) sequences showed high similarity (> 99%) to P. brasiliense sequences. To further validate this identification, Pcb-specific primers BR1f/L1r was used for PCR, and it produced a predicted amplicon of 322 bp expected for P. brasiliense (Duarte et al. 2004). All five isolates could be detected by BR1f/L1r primer. To fulfill Koch's postulates, five healthy Conophytum luiseae were inoculated by spraying bacterial inoculum (108 CFU/ml), meanwhile five additional healthy Conophytum luiseae were implemented with sterilized distilled water as a negative control. The plants were then kept at 70% humidity and 25ºC. Seven days after inoculation, the inoculated plants showed serious soft rot, while the control samples remained healthy. Bacteria were re-isolated from rot of inoculated tissues, and the isolates were identified as the original pathogen by the 16S rRNA gene sequences. P. Brasiliense has been reported to cause soft rot on diverse plant hosts, like sweet potato, radish, tobacco (Liu et al. 2019; Voronina et al., 2019; Wang et al., 2017). Best to our knowledge, this is the first report that P. Brasiliense causes soft rot on Conophytum luiseae in China.
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http://dx.doi.org/10.1094/PDIS-03-22-0541-PDNDOI Listing
May 2022

The transcription factor bZIP68 negatively regulates cold tolerance in maize.

Plant Cell 2022 Jul;34(8):2833-2851

State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, Center for Crop Functional Genomics and Molecular Breeding, China Agricultural University, Beijing 100193, China.

Maize (Zea mays) originated in tropical areas and is thus susceptible to low temperatures, which pose a major threat to maize production. Our understanding of the molecular basis of cold tolerance in maize is limited. Here, we identified bZIP68, a basic leucine zipper (bZIP) transcription factor, as a negative regulator of cold tolerance in maize. Transcriptome analysis revealed that bZIP68 represses the cold-induced expression of DREB1 transcription factor genes. The stability and transcriptional activity of bZIP68 are controlled by its phosphorylation at the conserved Ser250 residue under cold stress. Furthermore, we demonstrated that the bZIP68 locus was a target of selection during early domestication. A 358-bp insertion/deletion (Indel-972) polymorphism in the bZIP68 promoter has a significant effect on the differential expression of bZIP68 between maize and its wild ancestor teosinte. This study thus uncovers an evolutionary cis-regulatory variant that could be used to improve cold tolerance in maize.
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http://dx.doi.org/10.1093/plcell/koac137DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338793PMC
July 2022

Overexpression of miRNA-93-5p Promotes Proliferation and Migration of Bladder Urothelial Carcinoma via Inhibition of KLF9.

Comput Math Methods Med 2022 9;2022:8911343. Epub 2022 Mar 9.

Provincial Clinical Medical College of Fujian Medical University, Fuzhou 350001, China.

We focused on studying the effects of a key miRNA-mRNA axis in bladder urothelial carcinoma (BUC). Firstly, miRNAs and mRNAs differentially expressed in BUC were analyzed. Clinical information in the TCGA database was used for survival analysis, and the regulator of miRNA-93-5p was predicted. miRNA-93-5p and KLF9 mRNA expression were detected by qRT-PCR. Protein level detection and targeting measurement were, respectively, achieved by western blot and dual-luciferase approaches. The proliferative, invasive, and migratory abilities were tested through CCK-8, Transwell, and wound healing methods. Cell apoptosis in each group was detected through flow cytometry. As discovered, miRNA-93-5p level was markedly high in BUC cells while KLF9 expression was remarkably low. miRNA-93-5p overexpression promoted BUC cell abilities. Besides, miRNA-93-5p inhibited KLF9 expression. Furthermore, KLF9 overexpression dramatically attenuated such promotion on cancer cell abilities. On the whole, miRNA-93-5p/KLF9 axis facilitated BUC progression, offering a new potential target for BUC patients.
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http://dx.doi.org/10.1155/2022/8911343DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9042641PMC
May 2022

EXOC4 Promotes Diffuse-Type Gastric Cancer Metastasis via Activating FAK Signal.

Mol Cancer Res 2022 07;20(7):1021-1034

Department of General Surgery, Zhongshan Hospital, General Surgery Research Institute, Fudan University, Shanghai, China.

In comparison with intestinal-type gastric cancer, diffuse-type gastric cancer (DGC) is more likely to recur, metastasize, and exhibit worse clinical outcomes; however, the underlying mechanism of DGC recurrence remains elusive. By employing an LC/MS-MS proteomic approach, we identified that exocyst complex component 4 (EXOC4) was significantly upregulated in DGC with recurrence, compared to those with nonrecurrence. High expression of EXOC4 was correlated with tumor metastasis and poor prognosis in patients with DGC. Moreover, EXOC4 promoted cell migration and invasion as well as the tumor metastasis of DGC cells. Mechanistically, EXOC4 regulated the phosphorylation of focal adhesion kinase (FAK) at Y397 sites by stimulating the secretion of integrin α5/β1/EGF and enhancing the interaction of FAK and integrin or EGFR. The FAK inhibitor VS-4718 reversed the metastasis mediated by EXOC4 overexpression and suppressed the tumor growth of patient-derived xenografts derived from DGC with high EXOC4 expression. The EXOC4-FAK axis could be a potential therapeutic target for patients with DGC with high expression of EXOC4.

Implications: The EXOC4-FAK axis promoted DGC metastasis and could be a potential therapeutic target for patients with DGC.
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http://dx.doi.org/10.1158/1541-7786.MCR-21-0441DOI Listing
July 2022

Lipidomic Signatures of Dairy Consumption and Associated Changes in Blood Pressure and Other Cardiovascular Risk Factors Among Chinese Adults.

Hypertension 2022 Aug 26;79(8):1617-1628. Epub 2022 Apr 26.

Shanghai Institute of Nutrition and Health (H.Y., L.S., Y.L., H.L., G.Z., X.L.), University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China.

Background: Omics data may provide a unique opportunity to discover dairy-related biomarkers and their linked cardiovascular health.

Methods: Dairy-related lipidomic signatures were discovered in baseline data from a Chinese cohort study (n=2140) and replicated in another Chinese study (n=212). Dairy intake was estimated by a validated food-frequency questionnaire. Lipidomics was profiled by high-coverage liquid chromatography-tandem mass spectrometry. Associations of dairy-related lipids with 6-year changes in cardiovascular risk factors were examined in the discovery cohort, and their causalities were analyzed by 2-sample Mendelian randomization using available genome-wide summary data.

Results: Of 350 lipid metabolites, 4 sphingomyelins, namely sphingomyelin (OH) C32:2, sphingomyelin C32:1, sphingomyelin (2OH) C30:2, and sphingomyelin (OH) C38:2, were identified and replicated to be positively associated with total dairy consumption (β=0.130 to 0.148; <1.43×10), but not or weakly with nondairy food items. The score of 4 sphingomyelins showed inverse associations with 6-year changes in systolic (-2.68 [95% CI, -4.92 to -0.43]; =0.019), diastolic blood pressures (-1.86 [95% CI, -3.12 to -0.61]; =0.004), and fasting glucose (-0.25 [95% CI, -0.41 to -0.08]; =0.003). Mendelian randomization analyses further revealed that genetically inferred sphingomyelin (OH) C32:2 was inversely associated with systolic (-0.57 [95% CI, -0.85 to -0.28]; =9.16×10) and diastolic blood pressures (-0.39 [95% CI, -0.59 to -0.20]; =7.09×10).

Conclusions: The beneficial effects of dairy products on cardiovascular health might be mediated through specific sphingomyelins among Chinese with overall low dairy consumption.
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http://dx.doi.org/10.1161/HYPERTENSIONAHA.122.18981DOI Listing
August 2022

Glutathione-Responsive Pyraclostrobin-Loaded Polyurea Microcapsules for Their Intelligent Controlled Release.

J Agric Food Chem 2022 May 25;70(17):5310-5318. Epub 2022 Apr 25.

Shanghai Engineering Research Center of Green Energy Chemical Engineering, College of Chemistry and Materials Science, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, P. R. China.

The utilization of intelligent controlled release technology to create stimuli-responsive pesticide formulations has been shown to effectively improve pesticide efficacy and reduce environmental pollution. Herein, a glutathione-responsive release polyurea (PU) microcapsules (MCs) loaded with pyraclostrobin were developed via the interface polymerization method. The pyraclostrobin-loaded PU-MCs showed a regular spherical shape with an average diameter of 480 nm. It also showed good thermal stability and rheological properties. Furthermore, the pyraclostrobin-loaded PU-MCs exhibited favorable wettability on wheat leaves, which was beneficial for enhancing the retention capacity of pesticide droplets and improving pesticide utilization. The pyraclostrobin can be released from MCs and directly proportional to glutathione (GSH) concentrations with Fickian diffusion. Importantly, the control efficacy of pyraclostrobin-loaded PU-MCs against was positively correlated with GSH, indicating a promising candidate for a controlled release of pesticides in agriculture and laying the foundation for further field experiments.
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http://dx.doi.org/10.1021/acs.jafc.1c08182DOI Listing
May 2022

Radical Acylalkylation of 1,3-Enynes To Access Allenic Ketones via -Heterocyclic Carbene Organocatalysis.

J Org Chem 2022 04 29;87(8):5229-5241. Epub 2022 Mar 29.

State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, P. R. China.

An -heterocyclic carbene organocatalytic 1,4-difunctionalization of 1,3-enynes was developed. This organocatalytic strategy was suitable for a broad spectrum of substrates to efficiently synthesize allenic ketones bearing diverse substituents. Preliminary mechanistic studies suggest a radical reaction pathway for this organocatalytic acylalkylation process.
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http://dx.doi.org/10.1021/acs.joc.2c00037DOI Listing
April 2022

The effect of mitochondrial fusion on chondrogenic differentiation of cartilage progenitor/stem cells via Notch2 signal pathway.

Stem Cell Res Ther 2022 03 25;13(1):127. Epub 2022 Mar 25.

Department of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University, School of Medicine, Hangzhou, 310000, Zhejiang Province, People's Republic of China.

Background: Osteoarthritis (OA) is a debilitating disease that inflicts intractable pain, a major problem that humanity faces, especially in aging populations. Stem cells have been used in the treatment of many chronic diseases, including OA. Cartilage progenitor/stem cells (CPSCs) are a type of stem cells with the ability to self- renew and differentiate. They hold a promising future for the understanding of the progression of OA and for its treatment. Previous studies have reported the relationship between mitochondrial dynamics and mesenchymal stem cell (MSC) proliferation, differentiation and aging. Mitochondrial dynamic and morphology change during stem cell differentiation.

Methods: This study was performed to access the relationship between mitochondrial dynamics and chondrogenic differentiation of CPSCs. Mitochondrial fusion and fission levels were measured during the chondrogenic differentiation process of CPSCs. After that, we used mitochondrial fusion promoter to induce fusion in CPSCs and then the chondrogenic markers were measured. Transmission electron microscopy (TEM) and confocal microscopy were used to capture the mass and fusion status of mitochondria. Lentiviruses were used to detect the role of mitofusin 2 (Mfn2) in CPSC chondrogenic differentiation. In vivo, Mfn2 was over-expressed in sheets of rat CPSCs, which were then injected intra-articularly into the knees of rats.

Results: Mitochondrial fusion markers were upregulated during the chondrogenic induction process of CPSCs. The mass of mitochondria was higher in differentiated CPSC, and the fusion status was obvious relative to un-differentiated CPSC. Chondrogenesis of CPSCs was upregulated with the induction by mitochondrial fusion promoter. Mfn2 over-expression significantly increased chondrocyte-specific gene expression and reversed OA through NOTCH2 signal pathway.

Conclusions: Our study demonstrated that the mitochondrial fusion promotes chondrogenesis differentiation of CPSCs. Mfn2 accelerates the chondrogenesis differentiation of CPSCs through Notch2. In vivo, Mfn2-OE in sheets of rCPSCs ameliorated OA in the rat model.
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http://dx.doi.org/10.1186/s13287-022-02758-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8951683PMC
March 2022

Visit-to-Visit Heart Rate Variability Is Positively Associated With the Risk of Adverse Cardiovascular Outcomes.

Front Cardiovasc Med 2022 7;9:850223. Epub 2022 Mar 7.

Cardiac Care Unit, Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.

Introduction: Previous studies found visit-to-visit heart rate variability (VVHRV) may be positively associated with risks of several cardiovascular events, but whether VVHRV affected the benefit of intensive blood pressure control remained unknown. In this study, we assessed the risk of the composite cardiovascular outcomes associated with VVHRV among the older patients with hypertension and evaluated whether the benefit of intensive blood pressure control in the prevention of the composite cardiovascular outcomes was consistent in the context of elevated VVHRV.

Methods: This was a analysis of the Systolic Blood Pressure Intervention Trial (SPRINT). We explored the relationship between VVHRV and the composite cardiovascular outcomes by multivariate Cox proportional hazard regressions. The primary endpoint was the composite cardiovascular outcomes, same as SPRINT, defined as a composite of myocardial infarction, stroke, heart failure, and/or death from cardiovascular causes. We used multiple adjustment models for all regressions.

Results: Nine thousand two hundred and fourty-seven patients from the SPRINT were included in our analysis. We found a positive association between VVHRV and the risk of composite cardiovascular outcomes among the elderly with hypertension. Per 1 CV increment in HRCV, the hazard ratio of the risk of composite cardiovascular outcomes was 1.04 (95CI: 1.03, 1.05) in the fully adjusted Model. The benefit of intensive blood pressure control in managing cardiovascular events was consistent in different VVHRV subgroups. There was no significant interaction in other confounders.

Conclusion: We found the VVHRV was associated with the composite cardiovascular outcomes among the elderly with hypertension, intensive blood pressure control did not change the above association, and the benefits of intensive blood pressure management were consistent across different VVHRV groups.
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http://dx.doi.org/10.3389/fcvm.2022.850223DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8936423PMC
March 2022

An Oxidant- and Catalyst-Free Electrooxidative Cross-Coupling Approach to Synthesize -Substituted Porphyrin Derivatives.

J Org Chem 2022 04 18;87(7):4742-4749. Epub 2022 Mar 18.

College of Pharmacy, Third Military of Medical University, Chongqing 400038, PR China.

The synthesis of porphyrin and chlorin derivatives has attracted significant attention due to their numerous applications. Herein, we report an environment friendly oxidant- and catalyst-free electrooxidative cross-coupling approach for multiple coupling reactions to synthesize C-N, C-O, and C-S substituted porphyrin and chlorin derivatives. For C-N cross-coupling reactions, diaminated porphyrins were obtained as the main products, while using 4-bromo-2,6-dimethyl aniline resulted in monoaminated product. Similarly, electrochemical catalysis of porphyrins with phenol and thiophene produced -disubstituted porphyrins in moderate yields under a smaller current. Chlorins were also applicable, and 20-substituted products were efficiently produced regioselectively. To the best of our knowledge, this work represents the first example of electrooxidative C-X cross-coupling of porphyrins and chlorins.
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http://dx.doi.org/10.1021/acs.joc.2c00031DOI Listing
April 2022

Baseline sensitivity of Rhizoctonia solani to four DMI fungicides.

J Basic Microbiol 2022 Jun 14;62(6):701-710. Epub 2022 Mar 14.

Shanghai Engineering Research Centre of Low-Carbon Agriculture, Institute of Eco-Environment and Plant Protection, Shanghai Academy of Agricultural Sciences, Shanghai, China.

A set of 78 Rhizoctonia solani isolates from Shanghai, China, were examined for their sensitivity in vitro to four demethylase inhibitor (DMI) fungicides, epoxiconazole, difenoconazole, hexaconazole, and tebuconazole, by the mycelial growth rate method. The isolates were very sensitive to the four DMI fungicides. The ranges and means of the EC values were 0.004-0.045 and 0.014 ± 0.007 μg ml for epoxiconazole, 0-0.103 and 0.010 ± 0.016 μg ml for difenoconazole, 0.001-0.078 and 0.011 ± 0.013 μg ml for hexaconazole, and 0.001-0.210 and 0.034 ± 0.035 μg ml for tebuconazole, respectively. The frequency distribution of the EC values showed a normal curve for epoxiconazole and an approximately unimodal curve for difenoconazole, hexaconazole, and tebuconazole. There was no correlation between the sensitivities of the R. solani population to any two of the four fungicides (r < 0.3). In field trials, in 2015 and 2016, the control efficacies of hexaconzole, epoxiconazole, and tebuconazole were >90.0% at 75 g.a.i. ha , 80.0%-90.0% at 75 g.a.i. ha and 80.0%-90.0% at 97 g.a.i. ha , respectively; these values are significantly higher (p < 0.05) than those obtained in this study for difenoconazole (65.0%-70.0% at 80 g.a.i. ha ) and jinggangmycin (68.0%-71.0% at 150 g.a.i. ha ). These results show that sensitivity data can be used to monitor and detect any variations in the sensitivity of the R. solani population to these four DMI fungicides, and demonstrate that epoxiconazole, hexaconazole, and tebuconazole are likely to be good alternatives to jinggangmycin for controlling rice sheath blight.
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http://dx.doi.org/10.1002/jobm.202100642DOI Listing
June 2022

HDL quality features revealed by proteome‒lipidome connectivity are associated with atherosclerotic disease.

J Mol Cell Biol 2022 Jul;14(3)

CAS Key Laboratory of Systems Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China.

Lipoprotein, especially high-density lipoprotein (HDL), particles are composed of multiple heterogeneous subgroups containing various proteins and lipids. The molecular distribution among these subgroups is closely related to cardiovascular disease (CVD). Here, we established high-resolution proteomics and lipidomics (HiPL) methods to depict the molecular profiles across lipoprotein (Lipo-HiPL) and HDL (HDL-HiPL) subgroups by optimizing the resolution of anion-exchange chromatography and comprehensive quantification of proteins and lipids on the omics level. Furthermore, based on the Pearson correlation coefficient analysis of molecular profiles across high-resolution subgroups, we achieved the relationship of proteome‒lipidome connectivity (PLC) for lipoprotein and HDL particles. By application of these methods to high-fat, high-cholesterol diet-fed rabbits and acute coronary syndrome (ACS) patients, we uncovered the delicate dynamics of the molecular profile and reconstruction of lipoprotein and HDL particles. Of note, the PLC features revealed by the HDL-HiPL method discriminated ACS from healthy individuals better than direct proteome and lipidome quantification or PLC features revealed by the Lipo-HiPL method, suggesting their potential in ACS diagnosis. Together, we established HiPL methods to trace the dynamics of the molecular profile and PLC of lipoprotein and even HDL during the development of CVD.
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http://dx.doi.org/10.1093/jmcb/mjac004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9254886PMC
July 2022

Upregulated YB-1 protein promotes glioblastoma growth through a YB-1/CCT4/mLST8/mTOR pathway.

J Clin Invest 2022 04;132(8)

State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry and Cell Biology, CAS Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China.

Y-box-binding protein 1 (YB-1) is a multifunctional RNA binding protein involved in virtually every step of RNA metabolism. However, the functions and mechanisms of YB-1 in one of the most aggressive cancers, glioblastoma, are not well understood. In this study, we found that YB-1 protein was markedly overexpressed in glioblastoma and acted as a critical activator of both mTORC1 and mTORC2 signaling. Mechanistically, YB-1 bound the 5'UTR of CCT4 mRNA to promote the translation of CCT4, a component of the CCT chaperone complex, that in turn activated the mTOR signaling pathway by promoting mLST8 folding. In addition, YB-1 autoregulated its own translation by binding to its 5'UTR, leading to sustained activation of mTOR signaling. In patients with glioblastoma, high protein expression of YB-1 correlated with increased expression of CCT4 and mLST8 and activated mTOR signaling. Importantly, the administration of RNA decoys specifically targeting YB-1 in a mouse xenograft model resulted in slower tumor growth and better survival. Taken together, these findings uncover a disrupted proteostasis pathway involving a YB-1/CCT4/mLST8/mTOR axis in promoting glioblastoma growth, suggesting that YB-1 is a potential therapeutic target for the treatment of glioblastoma.
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http://dx.doi.org/10.1172/JCI146536DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9012288PMC
April 2022

Characteristics of Heteroresistance in Gastric Biopsies and Its Clinical Relevance.

Front Cell Infect Microbiol 2021 4;11:819506. Epub 2022 Feb 4.

Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang, China.

Background: Antimicrobial susceptibility testing (AST) plays a vital role in anti- treatment, but the traditional AST method has difficulty detecting heteroresistance, which may cause an increased prevalence of resistant strains and eradication failure.

Aims: To investigate the characteristics of heteroresistance in in gastric biopsies and investigate its clinical relevance.

Method: A total of 704 gastric biopsies were selected for 23S rRNA and gyrA gene sequencing, 470 isolates from these biopsies were selected for AST, and the clinical characteristics of the patients were reviewed.

Result: For the 699 biopsies that were positive for 23S rRNA gene, 98 (14.0%) showed a heteroresistance genotype, and a wild type (WT) combined with A2143G (86.7%) genotype was found in most samples. For the 694 biopsies that were positive for gyrA gene, 99 (14.3%) showed a heteroresistance genotype, and a WT combined with 87K (26.3%) or WT combined with 91N (23.2%) genotype was predominant. According to the E-test results, the resistance rates of heteroresistance genotype samples for clarithromycin and levofloxacin were 36.2% and 68.1%, respectively. When dividing the heteroresistance samples into different groups according to the sequencing profile peaks of the mutation position, the resistance rates were higher along with mutation peaks at the mutation position. In addition, patients infected with mutated or heteroresistant strains showed lower peptic ulcer detection rates than those infected with the WT strain (p < 0.05).

Conclusion: Heteroresistance genotypes for clarithromycin and levofloxacin were not rare in . Most cases with a heteroresistance genotype showed a susceptible phenotype for clarithromycin and a resistance phenotype for levofloxacin. Patients infected with heteroresistance genotype strains showed a lower peptic ulcer detection rate than those infected with the WT strain.
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http://dx.doi.org/10.3389/fcimb.2021.819506DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8855363PMC
April 2022

Altered Neurovascular Coupling in Unilateral Pulsatile Tinnitus.

Front Neurosci 2021 21;15:791436. Epub 2022 Jan 21.

Department of Radiology, Beijing Friendship Hospital, Capital Medical University, Beijing, China.

Objective: Altered cerebral blood flow (CBF) and regional homogeneity (ReHo) have been reported in pulsatile tinnitus (PT) patients. We aimed to explore regional neurovascular coupling changes in PT patients.

Materials And Methods: Twenty-four right PT patients and 25 sex- and age-matched normal controls were included in this study. All subjects received arterial spin labeling imaging to measure CBF and functional MRI to compute ReHo. CBF/ReHo ratio was used to assess regional neurovascular coupling between the two groups. We also analyzed the correlation between CBF/ReHo ratio and clinical data from the PT patients.

Results: PT patients exhibited increased CBF/ReHo ratio in left middle temporal gyrus and right angular gyrus than normal controls, and no decreased CBF/ReHo ratio was found. CBF/ReHo ratio in the left middle temporal gyrus of PT patients was positively correlated with Tinnitus Handicap Inventory score ( = 0.433, = 0.035).

Conclusion: These findings indicated that patients with PT exhibit abnormal neurovascular coupling, which provides new information for understanding the neuropathological mechanisms underlying PT.
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http://dx.doi.org/10.3389/fnins.2021.791436DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8815060PMC
January 2022
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