Publications by authors named "Rong Wang"

2,472 Publications

  • Page 1 of 1

Thermo-responsive nonionic amphiphilic copolymers as draw solutes in forward osmosis process for high-salinity water reclamation.

Water Res 2022 Jun 16;221:118768. Epub 2022 Jun 16.

Singapore Membrane Technology Centre, Nanyang Environment and Water Research Institute, Nanyang Technological University, 1 Cleantech Loop, Singapore 637141, Singapore; School of Civil and Environmental Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore. Electronic address:

Recently, thermo-responsive nonionic amphiphilic copolymers have shown a great potential as forward osmosis (FO) draw solutes for high-salinity water desalination and zero-liquid discharge (ZLD). However, the relationship between the copolymer structural properties and key characteristics as draw solutes, as well as copolymer's chemical stability after regeneration have not been much studied. In this work, we systematically investigated poly (ethylene oxide)-block-poly (propylene oxide)-block-poly (ethylene oxide) (PEO-PPO-PEO) copolymers as draw solute. The results showed that the PEO segments significantly influenced the viscosity, osmotic pressure and lowest phase separation temperature of the copolymer aqueous solutions. Among four commercial copolymers studied, Pluronic® L35 with moderate molecular weight (Mn 1,900 Da), 50% PEO, and relatively high hydrophilic-lipophilic balance (HLB) showed the best draw solution (DS) performance. It also showed great stability in physiochemical properties and draw capacity after more than ten cycles of regeneration. On the other hand, despite the fact that membrane fouling was observed due to the use of copolymer DS, the FO flux (∼1.2 L m h, as similar with the virgin membrane) was not affected when high-salinity feedwater such as seawater RO brine was applied. Overall, our study has provided a more comprehensive understanding on the characteristics of nonionic amphiphilic copolymer DS and showcased the promise of copolymer-driven FO process in high-salinity water desalination and ZLD.
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http://dx.doi.org/10.1016/j.watres.2022.118768DOI Listing
June 2022

The genome of Prunus humilis provides new insights to drought adaption and population diversity.

DNA Res 2022 Jun 25. Epub 2022 Jun 25.

Institute of Horticulture, Ningxia Academy of Agricultural and Forestry Sciences, Yinchuan, 750012, China.

Prunus humilis (2n =2x = 16) is dwarf shrub fruit tree native to China and distributed widely in the cold and arid northern region. In this study, we obtained the whole genome sequences of P. humilis by combining Illumina, Pacbio and HiC sequencing technologies. This genome was 254.38Mb long and encodes 28,301 putative proteins. Phylogenetic analysis indicated that P. humilis shares the same ancestor with Prunus mume and Prunus armeniaca at ∼ 29.03 Mya. Gene expansion analysis implied that the expansion of WAX-related and LEA genes might be associated with high drought tolerance of P. humilis and LTR maybe one of the driver factors for the drought adaption by increase the copy number of LEAs. Population diversity analysis among 20 P. humilis accessions found that the genetic diversity of P. humilis populations was limited, only 1.40% base pairs were different with each other, more wild resources need to be collected and utilized in the breeding and improvement. This study provides new insights to the drought adaption and population diversity of P. humilis, that could be used as a potential model plant for horticultural research.
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http://dx.doi.org/10.1093/dnares/dsac021DOI Listing
June 2022

Gross Tumor Volume Predicts Survival and Pathological Complete Response of Locally Advanced Esophageal Cancer After Neoadjuvant Chemoradiotherapy.

Front Oncol 2022 7;12:898383. Epub 2022 Jun 7.

Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Background: Neoadjuvant chemoradiotherapy (neo-CRT) plus surgery has greatly improved the prognosis of locally advanced esophageal cancer (EC) patients. But which factors may influence the pathological tumor response and long-term survival remains unclear. The purpose of this study was to identify the prognostic biomarkers of locally advanced EC patients receiving neo-CRT.

Methods: We reviewed the data of 72 patients with cT2-4N0-3M0 EC who underwent neo-CRT at our hospital. The patients received intensity-modulated radiation therapy with a total radiation dose of 41.4-60.0 Gy. Most patients received platinum + paclitaxel-based combination regimens every three weeks for 2-4 cycles. The recorded data included age, sex, smoking history, alcohol use, histology, tumor location, clinical TNM stage, tumor length, gross tumor volume (GTV), GTV of primary tumor (GTVp), GTV of lymph nodes (GTVn), radiation dose, and number of chemotherapy cycles. Overall survival (OS), progression-free survival (PFS), and pathological complete response (pCR) were analyzed.

Results: The 3-year OS and PFS rates of these patients who underwent neo-CRT were 51.14% and 43.28%, respectively. In the univariate analyses, smoking history, clinical stage, GTV, GTVp, and GTVn were significantly associated with OS, whereas alcohol use, GTV, GTVp, and GTVn were significantly associated with PFS. Furthermore, in the multivariate analysis, GTV was an independent prognostic predictor of OS (hazard ratio (HR): 14.14, 95% confidence interval (CI): 3.747-53.33, < 0.0001) and PFS (HR: 6.090, 95% CI: 2.398-15.47, < 0.0001). In addition, GTV < 60.50 cm compared to > 60.50 cm was significantly associated with higher pCR rate (59.3% and 27.8%, respectively, = 0.038). High dose (> 50 Gy) and increased number of chemotherapy cycles (≥ 3) didn't improve the OS or PFS in patients with GTV > 60.50 cm.

Conclusion: GTV was an independent prognostic factor of long-term survival in EC patients, which may be because GTV is associated with histological response to neo-CRT. Additionally, patients with GTV > 60.50 cm didn't benefit from increased radiation dose or increased number of chemotherapy cycles.
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http://dx.doi.org/10.3389/fonc.2022.898383DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9209710PMC
June 2022

Assessing the Capability of Government Information Intervention and Socioeconomic Factors of Information Sharing during the COVID-19 Pandemic: A Cross-Country Study Using Big Data Analytics.

Behav Sci (Basel) 2022 Jun 15;12(6). Epub 2022 Jun 15.

Department of Human and Organizational Development, Peabody College of Education and Human Development, Vanderbilt University, Nashville, TN 37240, USA.

(1) Background: This study introduces a novel computational approach to examine government capabilities in information intervention for risk management, influential agents in a global information network, and the socioeconomic factors of information-sharing behaviors of the public across regions during the COVID-19 pandemic. (2) Methods: Citation network analysis was employed to gauge the online visibility of governmental health institutions across regions. A bipartite exponential random graph modeling (ERGM) procedure was conducted to measure network dynamics. (3) Results: COVID-19 response agencies in Europe had the highest web impact, whereas health agencies in North America had the lowest. Various stakeholders, such as businesses, non-profit organizations, governments, and educational institutions played a key role in sharing the COVID-19 response by agencies' information given on their websites. Income inequality and GDP per capita were associated with the high online visibility of governmental health agencies. Other factors, such as population size, an aging population, death rate, and case percentage, did not contribute to the agencies' online visibility, suggesting that demographic characteristics and health status are not predictors of sharing government resources. (4) Conclusions: A combination of citation network analysis and ERGM helps reveal information flow dynamics and understand the socioeconomic consequences of sharing the government's COVID-19 information during the pandemic.
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http://dx.doi.org/10.3390/bs12060190DOI Listing
June 2022

Exploration for the Optical Properties and Fluorescent Prediction of Nitrotriazole and Nitrofurazan: First-Principles and TD-DFT Calculations.

ACS Omega 2022 Jun 3;7(23):19694-19705. Epub 2022 Jun 3.

School of Chemistry and Chemical Engineering, Southwest Petroleum University, Chengdu 610500, Sichuan, China.

High-energy materials containing azole and furazan have revealed numerous properties; however, the underlying optical properties need to be solved. Meanwhile, the uncertainty for the choice of fluorescent matrix materials and the flexible situational conditions prompted us to estimate the optical and fluorescent properties of 5,5'-dinitro-2,2'-3,3'-bi-1,2,4-triazole (), 4,4'-dinitroazolefurazan (), and 4,4'-dinitro-3,3'-4,3'-ter-1,2,5-oxadiazole (). The first-principles calculation with improved dispersion correction terms and time-dependent density functional theory were utilized to calculate the absorbance and excitation energy of , , and , as well as characterization for their crystal structure, electronic structure, molecular orbitals, and so forth, synchronously. In this work, the absorbance anisotropy of and is stronger than that of . The absorbance for each of the (0,0,1) crystal planes in the three compounds is greater than that of the other two crystal planes. Moreover, has the maximum absorbance on the (0,0,1) crystal plane. The N-N-H from and N-O-N from and are responsible for these results, while N=N in weakens the performance of N-O-N. UV-vis spectra show that the maximum absorption wavelengths λ for , , and are 225, 228, and 201 nm, respectively. The number of five-membered rings and the coplanarity of groups in the intermolecular non-conjugation interaction potentially improve this ability due to the results from the crystal diffraction analysis. In addition, the polarization rate > > based on the molecular orbital analysis and the electrostatic potential calculation implies that the excitation energy of is less than , and the excitation energy of is less than . This work is beneficial to the expansion of energetic materials into the optical field and the accelerated application process of the related industry.
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http://dx.doi.org/10.1021/acsomega.2c01438DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202023PMC
June 2022

Effects of Light Spectra on Morphology, Gaseous Exchange, and Antioxidant Capacity of Industrial Hemp.

Front Plant Sci 2022 2;13:937436. Epub 2022 Jun 2.

School of Agriculture, Yunnan University, Kunming, China.

One of the most important growth factors in cannabis cultivation is light which plays a big role in its successful growth. However, understanding that how light controls the industrial hemp growth and development is poor and needs advanced research. Therefore, a pot study was conducted to investigate the effects of different colors of light, that is, white light (WL), blue light (BL), red light (RL), and 50% red with 50% blue mix light (RBL) on morphology, gaseous exchange and antioxidant capacity of industrial hemp. Compared with WL, BL significantly increase hemp growth in terms of shoot fresh biomass (15.1%), shoot dry biomass (27.0%), number of leaves per plant (13.7%), stem diameter (10.2%), root length (6.8%) and chlorophyll content (7.4%). In addition, BL promoted net photosynthesis, stomatal conductance, and transpiration, while reduces the lipid peroxidation and superoxide dismutase and peroxidase activities. However, RL and RBL significantly reduced the plant biomass, gas exchange parameters with enhanced antioxidant enzymes activities. Thus, blue light is useful for large-scale sustainable production of industrial hemp.
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http://dx.doi.org/10.3389/fpls.2022.937436DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201404PMC
June 2022

Hyperhomocysteinemia Is a Predictor for Poor Postoperative Angiogenesis in Adult Patients With Moyamoya Disease.

Front Neurol 2022 2;13:902474. Epub 2022 Jun 2.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Background And Purposes: The risk factors of poor postoperative angiogenesis in moyamoya disease (MMD) patients remain unknown. We aimed to investigate the association between hyperhomocysteinemia (HHcy) and postoperative angiogenesis of adult patients with MMD.

Methods: A total of 138 adult patients with MMD were prospectively recruited from July 1 to December 31, 2019. After excluding 10 patients accepting conservative therapy and 77 individuals without postoperative digital subtraction angiography (DSA), all 51 MMD patients were enrolled, and 28 patients received bilateral operations separately. Patients were grouped according to postoperative angiogenesis and HHcy presentation, respectively. Clinical data and laboratory examinations were compared. Potential risk factors were evaluated by univariate and multivariate logistic regression analysis. Nomogram was further performed. The biological functions of homocysteine (Hcy) were explored .

Results: Comparing to the normal, patients with poor postoperative angiogenesis were higher in serum Hcy ( = 0.004), HHcy ratio ( = 0.011), creatinine (Cr) ( < 0.001), uric acid (UA) ( = 0.036), Triglyceride ( = 0.001), high-density lipoprotein cholesterol (HDL-C) ( = 0.001), low-density lipoprotein cholesterol (LDL-C) ( = 0.009), ApoA ( = 0.022), apolipoprotein B (ApoB) ( = 0.013). Furthermore, HHcy was more common in men ( = 0.003) than women. Logistic analysis results showed that Hcy ( = 0.817, 95% = 0.707-0.944, = 0.006) was an independent risk factor. HHcy and Cr were significantly associated with poor postoperative angiogenesis in MMD patients. Further, Hcy could inhibit the proliferation, migration, and tube formation of human brain microvascular endothelial cells (HBMECs), which can be reversed by vascular endothelial growth factor (VEGF).

Conclusion: The HHcy was significantly correlated with poor postoperative angiogenesis in adult patients with MMD. Hcy significantly inhibits HBMECs proliferation, migration, and tube formation. Furthermore, VEGF could reverse the inhibition effect induced by Hcy. Lowering the level of Hcy may be beneficial for postoperative MMD patients. Focusing on the pathophysiology and mechanism of HHcy might help to guide postoperative clinical management.
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http://dx.doi.org/10.3389/fneur.2022.902474DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201052PMC
June 2022

Hispanic Ethnicity Differences in Birth Characteristics, Maternal Birth Place, and Risk of Early-Onset Hodgkin Lymphoma: A Population-Based Case-Control Study.

Cancer Epidemiol Biomarkers Prev 2022 Jun 16. Epub 2022 Jun 16.

Yale School of Public Health, New Haven, CT, United States.

Background: Hispanic ethnicity differences in the risk of early-onset Hodgkin lymphoma (HL) diagnosed at < 40 years are understudied. We conducted a population-based case-control study to evaluate associations between birth characteristics and early-onset HL with a focus on potential ethnic differences.

Methods: This study included 1,651 non-Hispanic White and 1,168 Hispanic cases with HL endorsing a range of races diagnosed at the age of 0-37 years during 1988-2015 and 140,950 controls without cancer matched on race/ethnicity and year of birth from the California Linkage Study of Early-Onset Cancers. Odds ratios (OR) and 95% confidence intervals (CI) were estimated from multivariable logistic regression models.

Results: Having a foreign-born mother versus a United States-born mother (i.e., the reference group) was associated with an increased risk of early onset HL among non-Hispanic Whites (OR=1.52, 95%CI:1.31-1.76; P<0.01) and a decreased risk among Hispanics (OR=0.78, 95%CI:0.69-0.88; P<0.01). Among both race groups, risk of early onset HL increased with birthweight and maternal age (all P-trends<0.01). Among Non-Hispanic Whites, each 5-year increase in maternal age and paternal age was associated with increased risk of early onset HL (OR=1.07, 95%CI:1.02-1.13; P-trend=0.01). Compared to female Hispanics, male Hispanics had an increased risk of early onset HL (OR =1.26, 95% CI:1.12-1.42; P<0.01).

Conclusion: Maternal birthplace may play a role in risk of early-onset HL that differs by ethnicity.

Impact: The ethnic differences observed between certain birth characteristics, maternal birthplace and early onset HL raise questions about the underlying biological, generational, lifestyle, residential, and genetic contributions to the disease.
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http://dx.doi.org/10.1158/1055-9965.EPI-22-0335DOI Listing
June 2022

Association between genetic variants of microRNA-21 and microRNA-155 and systemic lupus erythematosus: A case-control study from a Chinese population.

J Clin Lab Anal 2022 Jun 16:e24518. Epub 2022 Jun 16.

Department of Laboratory Medicine, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China.

Background: Systemic lupus erythematosus (SLE) is a common autoimmune disease, and its pathogenesis remains unclear. The alteration of genetic materials is believed to play a role in SLE development. This study evaluated the association between the genetic variants of microRNA-21 (miR-21) and microRNA-155 (miR-155) and SLE.

Methods: The SNaPshot genotyping method was used to detect the genotypes of selected SNPs in patients and controls. The expression of miR-21 and miR-155 was analyzed using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The functional annotation and the biological effects of SNPs were assessed by HaploReg V4.1 and Regulome DB V2.0 software. The Hardy-Weinberg equilibrium test was used to gather statistics, and odds ratios (ORs) and 95% confidence intervals (CIs) were evaluated by logistic regression.

Results: The distribution difference of TA genotype in rs767649 was observed (TA vs. T/T: OR = 0.68, 95%CI, 0.48-0.95, p = 0.026). There was a significant difference in the T/A + A/A (T/A + A/A vs. T/T: OR = 0.68, 95%CI, 0.49-0.94, p = 0.020). A significant difference in T allele distribution was found in the depressed complement of SLE (T vs. A: OR = 0.67, 95%CI, 0.47-0.95, p = 0.026). There were significant differences in genetic variants of rs13137 between the positive and the negative SSB antibodies (Anti-SSB) (T vs. A: OR = 0.67, 95%CI, 0.47-0.95, p = 0.026; T/A + T/T vs. AA: OR = 2.23, 1.18-4.49, p = 0.013). The expression levels of miR-21 and miR-155 were significantly higher in patients than in controls (p < 0.001).

Conclusions: This study provides novel insight that genetic variants of rs767649 and rs13137 are associated with susceptibility to SLE.
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http://dx.doi.org/10.1002/jcla.24518DOI Listing
June 2022

Exploring the impact of energy factor prices and environmental regulation on China's green innovation efficiency.

Environ Sci Pollut Res Int 2022 Jun 15. Epub 2022 Jun 15.

Business School, Nanjing Xiaozhuang University, No.3601 Hongjing Avenue, Jiangning District, Nanjing, 211171, Jiangsu, China.

This study adopts super-DEA model to measure the current level of green innovation efficiency in Chinese provinces. At the same time, Tobit model is used to analyze the impact of energy factor prices and environmental regulation on its efficiency, the results are as follows: (1) Chinese green innovation efficiency average is 0.545, which is at a low level, but shows a steady upward trend. The results have shown that large differences exist in different provinces. Average value of efficiency is highest in the eastern and lowest in the western, and low green innovation efficiency is focus on main provinces of producing coal-based mineral resources. (2) The price change of energy factors inhibits the national green innovation efficiency. The impact on the eastern part of China is positive, but did not pass the significance test, and in central region is negative and also did not pass test, but in the western, it inhibits green innovation efficiency significantly. Environmental regulation has a negative effect on the national green innovation efficiency, the impact coefficient of east is positive, and also positive in the central part, but it fails to pass significance test, while in the west is negative. It can be seen that there are significant regional differences in the impact of the two on the efficiency of green innovation. This paper proposes that we can pay attention to the promotion of environmental regulation, encourages enterprises to actively carry out green innovation activities, continues to deepen the market-oriented reform of energy prices, and increases credit support and introduce financial resources for corporate R&D activities to explore policies to improve the efficiency of regional green innovation.
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http://dx.doi.org/10.1007/s11356-022-21371-yDOI Listing
June 2022

The transcription factor OsMYBc and an E3 ligase regulate expression of a K+ transporter during salt stress.

Plant Physiol 2022 Jun 13. Epub 2022 Jun 13.

National Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.

Na+ and K+ homeostasis is essential for plant survival in saline soils. A member of the High-Affinity K+ Transporter (HKT) family in rice (Oryza sativa), OsHKT1;1, is a vital regulator of Na+ exclusion from shoots and is bound by a MYB transcription factor (OsMYBc). Here, we generated transgenic rice lines in the oshkt1;1 mutant background for genetic complementation using genomic OsHKT1;1 containing a native (Com) or mutated (mCom) promoter that cannot be bound by OsMYBc. In contrast to wild-type (WT) or Com lines, the mCom lines were not able to recover the salt-sensitive phenotype of oshkt1;1. The OsMYBc-overexpressing plants were more tolerant to salt stress than WT plants. A yeast two-hybrid screen using the OsMYBc N terminus as bait identified a rice MYBc stress-related RING finger protein (OsMSRFP). OsMSRFP is an active E3 ligase that ubiquitinated OsMYBc in vitro and mediated 26S proteasome-mediated degradation of OsMYBc under semi-in vitro and in vivo conditions. OsMSRFP attenuated OsMYBc-mediated OsHKT1;1 expression, and knockout of OsMSRFP led to rice salt tolerance. These findings uncover a regulatory mechanism of salt response that fine-tunes OsHKT1;1 transcription by ubiquitination of OsMYBc.
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http://dx.doi.org/10.1093/plphys/kiac283DOI Listing
June 2022

One-step coordination of metal-phenolic networks as antibacterial coatings with sustainable and controllable copper release for urinary catheter applications.

RSC Adv 2022 May 25;12(25):15685-15693. Epub 2022 May 25.

Zhejiang International Scientific and Technological Cooperative Base of Biomedical Materials and Technology, Zhejiang Engineering Research Center for Biomedical Materials, Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences Ningbo 315300 China

Catheter-associated urinary tract infections (CAUTIs) draw great concern due to increased demand for urinary catheters in hospitalization. Encrustation caused by urinary pathogens, especially , results in blocking of the catheter lumen and further infections. In this study, a facile and low-cost surface modification strategy of urinary catheters was developed using one-step coordination of tannic acid (TA) and copper ions. The copper content of the coating could be manipulated by the number of TA-Cu (TC) layers, and the coating released copper in a pH-responsive manner. The coating exhibited high antibacterial efficiency (killed >99% of planktonic bacteria, and reduced biofilm coverage to <1% after 24 h) due to the synergistic antimicrobial effect of TA and copper ions. study with a rabbit model indicated that with two TC layers, the coated catheter could effectively inhibit bacterial growth in urine and colonization on the surface, and reduce encrustation formation. In addition, the TC-coated catheter exhibited better tissue compatibility compared to the unmodified catheter, probably due to the antibacterial performance of the coating. Such a straightforward coating strategy with good and antibacterial properties and biocompatibility holds great promise for combating CAUTIs in clinical practice.
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http://dx.doi.org/10.1039/d2ra01675cDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9132196PMC
May 2022

Diagnostic Accuracy of Wireless Capsule Endoscopy in Polyp Recognition Using Deep Learning: A Meta-Analysis.

Int J Clin Pract 2022 19;2022:9338139. Epub 2022 Mar 19.

Digestive Endoscopy Center, Shanxi Provincial People's Hospital, Taiyuan, China.

Aim: As the completed studies have small sample sizes and different algorithms, a meta-analysis was conducted to assess the accuracy of WCE in identifying polyps using deep learning.

Method: Two independent reviewers searched PubMed, Embase, the Web of Science, and the Cochrane Library for potentially eligible studies published up to December 8, 2021, which were analysed on a per-image basis. STATA RevMan and Meta-DiSc were used to conduct this meta-analysis. A random effects model was used, and a subgroup and regression analysis was performed to explore sources of heterogeneity.

Results: Eight studies published between 2017 and 2021 included 819 patients, and 18,414 frames were eventually included in the meta-analysis. The summary estimates for the WCE in identifying polyps by deep learning were sensitivity 0.97 (95% confidence interval (CI), 0.95-0.98); specificity 0.97 (95% CI, 0.94-0.98); positive likelihood ratio 27.19 (95% CI, 15.32-50.42); negative likelihood ratio 0.03 (95% CI 0.02-0.05); diagnostic odds ratio 873.69 (95% CI, 387.34-1970.74); and the area under the sROC curve 0.99.

Conclusion: WCE uses deep learning to identify polyps with high accuracy, but multicentre prospective randomized controlled studies are needed in the future.
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http://dx.doi.org/10.1155/2022/9338139DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9159236PMC
June 2022

The identification and validation of EphA7 hypermethylation, a novel biomarker, in cervical cancer.

BMC Cancer 2022 Jun 9;22(1):636. Epub 2022 Jun 9.

Department of Laboratory Medicine, Tianjin Medical University, Tianjin, China.

Background: Aberrant methylation of EphA7 has been reported in the process of carcinogenesis but not in cervical cancer. Therefore, an integration study was performed to explore the association between EphA7 hypermethylation and cervical cancer and validate the potential value of EphA7 hypermethylation in the diagnosis of cervical cancer.

Methods: We performed an integration study to identify and validate the association between EphA7 methylation and cervical cancer. First, data on EphA7 methylation and expression in cervical cancer were extracted and analyzed via bioinformatics tools. Subsequently, CRISPR-based methylation perturbation tools (dCas9-Tet1/DNMT3a) were constructed to further demonstrate the association between DNA methylation and EphA7 expression. Ultimately, the clinical value of EphA7 methylation in cervical cancer was validated in cervical tissues and Thinprep cytologic test (TCT) samples by methylation-specific PCR (MSP) and quantitative methylation-specific PCR (QMSP), respectively.

Results: Pooled analysis showed that EphA7 promoter methylation levels were significantly increased in cervical cancer compared to normal tissues (P < 0.001) and negatively correlated with EphA7 expression. These prediction results were subsequently confirmed in cell lines; moreover, CRISPR-based methylation perturbation tools (dCas9-Tet1/DNMT3a) demonstrated that DNA methylation participates in the regulation of EphA7 expression directly. Consistent with these findings, the methylation level and the positive rate of EphA7 gradually increased with severity from normal to cancer stages in TCT samples (P < 0.01).

Conclusions: EphA7 hypermethylation is present in cervical cancer and is a potential biomarker for the diagnosis of cervical cancer.
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http://dx.doi.org/10.1186/s12885-022-09653-7DOI Listing
June 2022

Tumor Microbiome in Nasopharyngeal Carcinoma and Its Association With Prognosis.

Front Oncol 2022 23;12:859721. Epub 2022 May 23.

Cancer Center, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, China.

Introduction: Previous studies have reported a close relationship between cancer and microbes, particularly gut and tumor microbiota; however, the presence of tumor microbiome in nasopharyngeal carcinoma (NPC) and its role in the prognosis of NPC remain unclear.

Methods: We collected 64 samples including tissues from 50 patients with NPC (NPC group) and 14 patients with chronic nasopharyngitis (control group) receiver operating characteristics and we applied 16S ribosome RNA gene sequencing of all samples to assess microbiome profiles and immunohistochemistry to detect tumor microbiome in NPC.

Results: Patients in the control group harbored higher species diversity than those in the NPC group; however, the beta diversity was more distinct in the NPC group. In total, three genera with statistically significant differences between the two groups were identified. The area under the receiver operating characteristics (ROC) curve (AUC) was calculated using the relative abundance of these three significant genera, and a value of 0.842 was achieved. Furthermore, was confirmed as a potentially independent prognostic factor for NPC patients, and the progression-free survival (PFS) was markedly prolonged in patients with a low relative abundance of compared to patients with a high relative abundance of this genus (cutoff: 0.0046, hazard ratio: 5.10, 95% confidence interval: 2.04-12.77, = 0.004).

Conclusions: The present study provided strong evidence of a correlation between tumor microbiome and NPC; the tumor microbiome may be considered a biomarker for early NPC diagnosis. potentially served as a independently prognostic indicator for NPC patients.
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http://dx.doi.org/10.3389/fonc.2022.859721DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9168217PMC
May 2022

Molecular basis of mEAK7-mediated human V-ATPase regulation.

Nat Commun 2022 Jun 7;13(1):3272. Epub 2022 Jun 7.

Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA.

The activity of V-ATPase is well-known to be regulated by reversible dissociation of its V and V domains in response to growth factor stimulation, nutrient sensing, and cellular differentiation. The molecular basis of its regulation by an endogenous modulator without affecting V-ATPase assembly remains unclear. Here, we discover that a lysosome-anchored protein termed (mammalian Enhancer-of-Akt-1-7 (mEAK7)) binds to intact V-ATPase. We determine cryo-EM structure of human mEAK7 in complex with human V-ATPase in native lipid-containing nanodiscs. The structure reveals that the TLDc domain of mEAK7 engages with subunits A, B, and E, while its C-terminal domain binds to subunit D, presumably blocking V-V torque transmission. Our functional studies suggest that mEAK7, which may act as a V-ATPase inhibitor, does not affect the activity of V-ATPase in vitro. However, overexpression of mEAK7 in HCT116 cells that stably express subunit a4 of V-ATPase represses the phosphorylation of ribosomal protein S6. Thus, this finding suggests that mEAK7 potentially links mTOR signaling with V-ATPase activity.
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http://dx.doi.org/10.1038/s41467-022-30899-zDOI Listing
June 2022

Insights gained from Single-Cell analysis of immune cells on Cyclosporine A treatment in autoimmune uveitis.

Biochem Pharmacol 2022 Jun 4;202:115116. Epub 2022 Jun 4.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangzhou 510060, China. Electronic address:

Cyclosporine A (CsA) is a widely known immunosuppressive agent that is clinically important in autoimmune diseases owing to its selective suppression of T lymphocytes. Although it has long been recognized to inhibit T cell responses by blocking calcineurin, the potential targets and specific downstream mechanisms remain elusive. Herein, we built a comprehensive single-cell transcriptomic landscape of immune cells in the blank, untreated experimental autoimmune uveitis (EAU), and CsA-treated EAU mice. CsA reversed EAU-associated changes in cell type composition, genomic expression, cell trajectory, and cell-cell communication. We found that CsA reverses the proportion change of disease-related immune cells; regulates several crucial pathogenic factors (eg. IL1r1, CD48, and Bhlhe40) in T helper 17 cells (Th17), the transcription factor Bhlhe40 was also rescued in T helper 1 cells (Th1); and may differentiate Tregs into a state of enhanced immunosuppression. In addition, we revealed the rescued impact of CsA on all immune cell types, especially on plasma B cells differentiation and immunoglobulin secretion. Furthermore, comparisons with glucocorticoids showed that CsA might have a more premium rescue effect involved in attenuating the pathogenicity of autoreactive T cells. Our work provides a comprehensive single-cell transcriptional atlas of immune cells under CsA therapy, providing advanced insights into the mechanisms underlying CsA and a reference for developing new therapeutic strategies for autoimmune diseases.
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http://dx.doi.org/10.1016/j.bcp.2022.115116DOI Listing
June 2022

Enhancement of L-ribulose Production from L-ribose Through Modification of Ochrobactrum sp. CSL1 Ribose-5-phosphate Isomerase A.

Appl Biochem Biotechnol 2022 Jun 7. Epub 2022 Jun 7.

School of Chemistry and Life Sciences, Suzhou University of Science and Technology, No. 99 Xuefu Rd., Huqiu District, Suzhou, Jiangsu Province, 215009, People's Republic of China.

L-ribulose, a kind of high-value rare sugar, could be utilized to manufacture L-form sugars and antiviral drugs, generally produced from L-arabinose as a substrate. However, the production of L-ribulose from L-arabinose is limited by the equilibrium ratio of the catalytic reaction, hence, it is necessary to explore a new biological enzymatic method to produce L-ribulose. Ribose-5-phosphate isomerase (Rpi) is an enzyme that can catalyze the reversible isomerization between L-ribose and L-ribulose, which is of great significance for the preparation of L-ribulose. In order to obtain highly active ribose-5-phosphate isomerase to manufacture L-ribulose, ribose-5-phosphate isomerase A (OsRpiA) from Ochrobactrum sp. CSL1 was engineered based on structural and sequence analyses. Through a rational design strategy, a triple-mutant strain A10T/T32S/G101N with 160% activity was acquired. The enzymatic properties of the mutant were systematically investigated, and the optimum conditions were characterized to achieve the maximum yield of L-ribulose. Kinetic analysis clarified that the A10T/T32S/G101N mutant had a stronger affinity for the substrate and increased catalytic efficiency. Furthermore, molecular dynamics simulations indicated that the binding of the substrate to A10T/T32S/G101N was more stable than that of wild type. The shorter distance between the catalytic residues of A10T/T32S/G101N and L-ribose illuminated the increased activity. Overall, the present study provided a solid basis for demonstrating the complex functions of crucial residues in RpiAs as well as in rare sugar preparation.
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http://dx.doi.org/10.1007/s12010-022-04015-2DOI Listing
June 2022

Acute stress promotes brain network integration and reduces state transition variability.

Proc Natl Acad Sci U S A 2022 Jun 6;119(24):e2204144119. Epub 2022 Jun 6.

Department of Management, Hong Kong Baptist University, Hong Kong.

Despite the prevalence of stress, how brains reconfigure their multilevel, hierarchical functional organization in response to acute stress remains unclear. We examined changes in brain networks after social stress using whole-brain resting-state functional MRI (fMRI) by extending our recently published nested-spectral partition method, which quantified the functional balance between network segregation and integration. Acute stress was found to shift the brain into a more integrated and less segregated state, especially in frontal-temporal regions. Stress also stabilized brain states by reducing the variability of dynamic transition between segregated and integrated states. Transition frequency was associated with the change of cortisol, and transition variability was correlated with cognitive control. Our results show that brain networks tend to be more integrated and less variable after acute stress, possibly to enable efficient coping.
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http://dx.doi.org/10.1073/pnas.2204144119DOI Listing
June 2022

Perioperative Myocardial Injury/Infarction After Non-cardiac Surgery in Elderly Patients.

Front Cardiovasc Med 2022 19;9:910879. Epub 2022 May 19.

Department of Comprehensive Surgery, General Hospital of Chinese People's Liberation Army and National Clinical Research Center for Geriatric Disease, Beijing, China.

At present, we have entered an aging society. Many diseases suffered by the elderly, such as malignant tumors, cardiovascular diseases, fractures, surgical emergencies and so on, need surgical intervention. With the improvement of Geriatrics, surgical minimally invasive technology and anesthesia level, more and more elderly patients can safely undergo surgery. Elderly surgical patients are often complicated with a variety of chronic diseases, and the risk of postoperative myocardial injury/infarction (PMI) is high. PMI is considered to be the increase of cardiac troponin caused by perioperative ischemia, which mostly occurs during operation or within 30 days after operation, which can increase the risk of short-term and long-term death. Therefore, it is suggested to screen troponin in elderly patients during perioperative period, timely identify patients with postoperative myocardial injury and give appropriate treatment, so as to improve the prognosis. The pathophysiological mechanism of PMI is mainly due to the increase of myocardial oxygen consumption and / the decrease of myocardial oxygen supply. Preoperative and postoperative risk factors of myocardial injury can be induced by mismatch of preoperative and postoperative oxygen supply. The treatment strategy should first control the risk factors and use the drugs recommended in the guidelines for treatment. Application of cardiovascular drugs, such as antiplatelet β- Receptor blockers, statins and angiotensin converting enzyme inhibitors can effectively improve postoperative myocardial ischemia. However, the risk of perioperative bleeding should be fully considered before using antiplatelet and anticoagulant drugs. This review is intended to describe the epidemiology, diagnosis, pathophysiology, risk factors, prognosis and treatment of postoperative myocardial infarction /injury.
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http://dx.doi.org/10.3389/fcvm.2022.910879DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9160386PMC
May 2022

Aligned Collagen-CNT Nanofibrils and the Modulation Effect on Ovarian Cancer Cells.

J Compos Sci 2021 Jun 2;5(6). Epub 2021 Jun 2.

Department of Chemistry, Illinois Institute of Technology, 3101 S. Dearborn St., Chicago, IL 60616, USA.

Fibrillar collagen is a one-dimensional biopolymer and is the most abundant structural protein in the extracellular matrix (ECM) of connective tissues. Due to the unique properties of carbon nanotubes (CNTs), considerable attention has been given to the application of CNTs in developing biocomposite materials for tissue engineering and drug delivery. When introduced to tissues, CNTs inevitably interact and integrate with collagen and impose a discernible effect on cells in the vicinity. The positive effect of the collagen-CNT (COL-CNT) matrix in tissue regeneration and the cytotoxicity of free CNTs have been investigated extensively. In this study, we aimed to examine the effect of COL-CNT on mediating the interaction between the matrix and SKOV3 ovarian cancer cells. We generated unidirectionally aligned collagen and COL-CNT nanofibrils, mimicking the structure and dimension of collagen fibrils in native tissues. AFM analysis revealed that the one-dimensional structure, high stiffness, and low adhesion of COL-CNT greatly facilitated the polarization of SKOV3 cells by regulating the β-1 integrin-mediated cell-matrix interaction, cytoskeleton rearrangement, and cell migration. Protein and gene level analyses implied that both collagen and COL-CNT matrices induced the epithelial-mesenchymal transition (EMT), and the COL-CNT matrix prompted a higher level of cell transformation. However, the induced cells expressed CD44 at a reduced level and MMP2 at an increased level, and they were responsive to the chemotherapy drug gemcitabine. The results suggested that the COL-CNT matrix induced the transdifferentiation of the epithelial cancer cells to mature, less aggressive, and less potent cells, which are inapt for tumor metastasis and chemoresistance. Thus, the presence of CNT in a collagen matrix is unlikely to cause an adverse effect on cancer patients if a controlled dose of CNT is used for drug delivery or tissue regeneration.
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http://dx.doi.org/10.3390/jcs5060148DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9164112PMC
June 2021

Chronic Kidney Disease and Cancer: Inter-Relationships and Mechanisms.

Front Cell Dev Biol 2022 18;10:868715. Epub 2022 May 18.

Department of Nephrology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China.

Chronic kidney disease (CKD) has been recognized as an increasingly serious public health problem globally over the decades. Accumulating evidence has shown that the incidence rate of cancer was relatively higher in CKD patients than that in general population, which, mechanistically, may be related to chronic inflammation, accumulation of carcinogenic compounds, oxidative stress, impairment of DNA repair, excessive parathyroid hormone and changes in intestinal microbiota, etc. And in patients with cancer, regardless of tumor types or anticancer treatment, it has been indicated that the morbidity and incidence rate of concomitant CKD was also increased, suggesting a complex inter-relationship between CKD and cancer and arousing increasing attention from both nephrologists and oncologists. This narrative review focused on the correlation between CKD and cancer, and underlying molecular mechanisms, which might provide an overview of novel interdisciplinary research interests and the potential challenges related to the screening and treatment of CKD and cancer. A better understanding of this field might be of help for both nephrologists and oncologists in the clinical practice.
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http://dx.doi.org/10.3389/fcell.2022.868715DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9158340PMC
May 2022

Drivers of foliar N trends in southern China over the last century.

Glob Chang Biol 2022 Jun 2. Epub 2022 Jun 2.

South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China.

Foliar stable nitrogen (N) isotopes (δ N) generally reflect N availability to plants and have been used to infer about changes thereof. However, previous studies of temporal trends in foliar δ N have ignored the influence of confounding factors, leading to uncertainties on its indication to N availability. In this study, we measured foliar δ N of 1811 herbarium specimens from 12 plant species collected in southern China forests from 1920 to 2010. We explored how changes in atmospheric CO , N deposition and global warming have affected foliar δ N and N concentrations ([N]) and identified whether N availability decreased in southern China. Across all species, foliar δ N significantly decreased by 0.82‰ over the study period. However, foliar [N] did not decrease significantly, implying N homeostasis in forest trees in the region. The spatiotemporal patterns of foliar δ N were explained by mean annual temperature (MAT), atmospheric CO ( ), atmospheric N deposition, and foliar [N]. The spatiotemporal trends of foliar [N] were explained by MAT, temperature seasonality, , and N deposition. N deposition within the rates from 5.3 to 12.6 kg N ha  year substantially contributed to the temporal decline in foliar δ N. The decline in foliar δ N was not accompanied by changes in foliar [N] and therefore does not necessarily reflect a decline in N availability. This is important to understand changes in N availability, which is essential to validate and parameterize biogeochemical cycles of N.
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http://dx.doi.org/10.1111/gcb.16285DOI Listing
June 2022

The Mutational, Epigenetic, and Transcriptional Effects Between Mixed High-Energy Particle Field (CR) and Li-Ion Beams (LR) Radiation in Wheat M Seedlings.

Front Plant Sci 2022 11;13:878420. Epub 2022 May 11.

Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Food Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan, China.

Ionizing radiation (IR) is an effective approach for mutation breeding. Understanding the mutagenesis and transcriptional profiles induced by different mutagens is of great significance for improving mutation breeding efficiency. Here, using RNA sequencing and methylation-sensitive amplification polymorphism (MSAP) approaches, we compared the genetic variations, epigenetics, and transcriptional responses induced by the mixed high-energy particle field (CR) and Li-ion beam (LR) radiation in M seedlings of two wheat genotypes (Yangmai 18 and Yangmai 20). The results showed that, in both wheat genotypes, CR displayed significantly a higher mutation efficiency (1.79 × 10/bp) than that by LR (1.56 × 10/bp). The induced mutations were not evenly distributed across chromosomes and varied across wheat genotypes. In Y18 M, the highest number of mutations were detected on Chr. 6B and Chr. 6D, whilst in Y20 M, Chr. 7A and Chr. 3A had the highest mutations. The transcript results showed that total of 4,755 CR-regulated and 1,054 LR-regulated differentially expressed genes (DEGs) were identified in the both genotypes. Gene function enrichment analysis of DEGs showed that these DEGs overlapped or diverged in the cascades of molecular networks involved in "phenylpropanoid biosynthesis" and "starch and sucrose metabolism" pathways. Moreover, IR type specific responses were observed between CR an LR irradiation, including specific TFs and response pathways. MSAP analysis showed that DNA methylation level increased in LR treatment, while decreased at CR. The proportion of hypermethylation was higher than that of hypomethylation at LR, whereas a reverse pattern was observed at CR, indicating that DNA methylation plays critical roles in response to IR irradiation. All these results support that the response to different IRs in wheat includes both common and unique pathways, which can be served as a useful resource to better understand the mechanisms of responses to different IRs in other plants.
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http://dx.doi.org/10.3389/fpls.2022.878420DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9131052PMC
May 2022

Neuroplasticity of Glioma Patients: Brain Structure and Topological Network.

Front Neurol 2022 13;13:871613. Epub 2022 May 13.

Department of Radiology, Huashan Hospital, Fudan University, Shanghai, China.

Glioma is the most common primary malignant brain tumor in adults. It accounts for about 75% of such tumors and occurs more commonly in men. The incidence rate has been increasing in the past 30 years. Moreover, the 5-year overall survival rate of glioma patients is < 35%. Different locations, grades, and molecular characteristics of gliomas can lead to different behavioral deficits and prognosis, which are closely related to patients' quality of life and associated with neuroplasticity. Some advanced magnetic resonance imaging (MRI) technologies can explore the neuroplasticity of structural, topological, biochemical metabolism, and related mechanisms, which may contribute to the improvement of prognosis and function in glioma patients. In this review, we summarized the studies conducted on structural and topological plasticity of glioma patients through different MRI technologies and discussed future research directions. Previous studies have found that glioma itself and related functional impairments can lead to structural and topological plasticity using multimodal MRI. However, neuroplasticity caused by highly heterogeneous gliomas is not fully understood, and should be further explored through multimodal MRI. In addition, the individualized prediction of functional prognosis of glioma patients from the functional level based on machine learning (ML) is promising. These approaches and the introduction of ML can further shed light on the neuroplasticity and related mechanism of the brain, which will be helpful for management of glioma patients.
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http://dx.doi.org/10.3389/fneur.2022.871613DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9136300PMC
May 2022

β-elemene regulates M1-M2 macrophage balance through the ERK/JNK/P38 MAPK signaling pathway.

Commun Biol 2022 05 31;5(1):519. Epub 2022 May 31.

Research Center for Food Safety, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, 113-8657, Japan.

Macrophages are classified into classically activated M1 macrophages and alternatively activated M2 macrophages, and the two phenotypes of macrophages are present during the development of various chronic diseases, including obesity-induced inflammation. In the present study, β-elemene, which is contained in various plant substances, is predicted to treat high-fat diet (HFD)-induced macrophage dysfunction based on the Gene Expression Omnibus (GEO) database and experimental validation. β-elemene impacts the imbalance of M1-M2 macrophages by regulating pro-inflammatory cytokines in mouse white adipose tissue both in vitro and in vivo. In addition, the RAW 264 cell line, which are macrophages from mouse ascites, is used to identify the effects of β-elemene on inhibiting bacterial endotoxin lipopolysaccharide (LPS)-induced phosphorylation of mitogen-activated protein kinase (MAPK) pathways. These pathways both induce and are activated by pro-inflammatory cytokines, and they also participate in the process of obesity-induced inflammation. The results highlight that β-elemene may represent a possible macrophage-mediated therapeutic medicine.
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http://dx.doi.org/10.1038/s42003-022-03369-xDOI Listing
May 2022

Enzymatic co-crosslinking of star-shaped poly(ethylene glycol) tyramine and hyaluronic acid tyramine conjugates provides elastic biocompatible and biodegradable hydrogels.

Bioact Mater 2023 Feb 20;20:53-63. Epub 2022 May 20.

Department of Developmental BioEngineering, Faculty of Science and Technology, Tech Med Centre, University of Twente, P.O. Box 217, 7500 AE, Enschede, the Netherlands.

A combination of the viscoelastic properties of hyaluronic acid (HA) and the elastic properties of star shaped 8-arm poly(ethylene glycol) (8-arm PEG) was used to design in-situ forming hydrogels. Hydrogels were prepared by the enzymatic crosslinking of a partially tyramine modified 8-arm PEG and a tyramine conjugated HA using horseradish peroxidase in the presence of hydrogen peroxide. Hydrogels of the homopolymer conjugates and mixtures thereof were rapidly formed within seconds under physiological conditions at low polymer and enzyme concentrations. Elastic hydrogels with high gel content (≥95%) and high storage moduli (up to 22.4 kPa) were obtained. An in vitro study in the presence of hyaluronidase (100 U/mL) revealed that with increasing PEG content the degradation time of the hybrid hydrogels increased up to several weeks, whereas hydrogels composed of only hyaluronic acid degraded within 2 weeks. Human mesenchymal stem cells (hMSCs) incorporated in the hybrid hydrogels remained viable as shown by a PrestoBlue and a live-dead assay, confirming the biocompatibility of the constructs. The production of an extracellular matrix by re-differentiation of encapsulated human chondrocytes was followed over a period of 28 days. Gene expression indicated that these highly elastic hydrogels induced an enhanced production of collagen type II. At low PEG-TA/HA-TA ratios a higher expression of SOX 9 and ACAN was observed. These results indicate that by modulating the ratio of PEG/HA, injectable hydrogels can be prepared applicable as scaffolds for tissue regeneration applications.
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http://dx.doi.org/10.1016/j.bioactmat.2022.05.020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9127275PMC
February 2023

PRRSV Induces HMGB1 Phosphorylation at Threonine-51 Residue to Enhance Its Secretion.

Viruses 2022 05 8;14(5). Epub 2022 May 8.

Laboratory Animal Center, Xi'an Jiaotong University, Xi'an 710061, China.

Porcine reproductive and respiratory syndrome virus (PRRSV) induces secretion of high mobility group box 1 (HMGB1) to mediate inflammatory response that is involved in the pulmonary injury of infected pigs. Our previous study indicates that protein kinase C-delta (PKC-delta) is essential for HMGB1 secretion in PRRSV-infected cells. However, the underlying mechanism in HMGB1 secretion induced by PRRSV infection is still unclear. Here, we discovered that the phosphorylation level of HMGB1 in threonine residues increased in PRRSV-infected cells. A site-directed mutagenesis study showed that HMGB1 phosphorylation at threonine-51 was associated with HMGB1 secretion induced by PRRSV infection. Co-immunoprecipitation (co-IP) of HMGB1 failed to precipitate PKC-delta, but interestingly, mass spectrometry analysis of the HMGB1 co-IP product showed that PRRSV infection enhanced HMGB1 binding to ribosomal protein S3 (RPS3), which has various extra-ribosomal functions. The silencing of RPS3 by siRNA blocked HMGB1 secretion induced by PRRSV infection. Moreover, the phosphorylation of HMGB1 at threonine-51 was correlated with the interaction between HMGB1 and RPS3. In vivo, PRRSV infection also increased RPS3 levels and nuclear accumulation in pulmonary alveolar macrophages. These results demonstrate that PRRSV may induce HMGB1 phosphorylation at threonine-51 and increase its interaction with RPS3 to enhance HMGB1 secretion. This finding provides insights into the pathogenesis of PRRSV infection.
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http://dx.doi.org/10.3390/v14051002DOI Listing
May 2022

Comparisons of Corn Stover Silages after Fresh- or Ripe-Corn Harvested: Effects on Digestibility and Rumen Fermentation in Growing Beef Cattle.

Animals (Basel) 2022 May 13;12(10). Epub 2022 May 13.

Key Laboratory for Agro-Ecological Processes in Subtropical Region/National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China.

Both waxy corn stover after fresh- (CF) and ripe-corn (CR) harvested are important byproducts of corn cropping system and have 20 d difference in harvest time. The study aimed to investigate the effects of prolonging harvest time on the nutritive value of corn stover silage by comparing CF with CR silages. In vitro ruminal experiment was firstly performed to investigate substrate degradation and fermentation of CF and CR silages. The CR diet was formulated by replacing 50% forage of CF silage with CR silage on a dry matter (DM) basis. Fourteen crossbred steers (Simmental × Limousin × local Chinese) aged 13 months with an average weight of 318.1 ± 37.1 kg were selected and randomly allocated into two dietary treatment groups. Although the CR silage had greater DM and fiber contents than CF silage, it did not alter in vitro degradation ( > 0.05), but with lower molar percentage of propionate and acetate to propionate ratio ( < 0.05). The cattle fed CR diet had a higher DM intake and lower fiber digestibility with reduction in 18S rRNA gene copies of protozoa and fungi and 16S rRNA gene copies of ( < 0.05). Further 16S rRNA gene amplicon analysis indicated a similar diversity of bacteria community between CR and CF treatments ( > 0.05). Few differences were observed in the abundance of genera larger than 1% ( > 0.05), except for the reduction in abundance of genera _NK4A214_group in CR treatment ( < 0.05). In summary, prolonging 20 d harvest time of corn stover silage increases the forage fiber and DM content, which promotes feed intake with decreased fiber degradation, although rumen fermentation and growth performance are not changed in growing beef cattle.
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http://dx.doi.org/10.3390/ani12101248DOI Listing
May 2022
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