Publications by authors named "Rong Ma"

406 Publications

Research on Characteristic of Chronic Spontaneous Urticaria Based on Multiscale Entropy.

Comput Math Methods Med 2021 25;2021:6691356. Epub 2021 May 25.

College of Mathematical Sciences, Harbin Engineering University, Harbin 150001, China.

Chronic spontaneous urticaria (CSU) is a common skin disease which symptom is local pruritus and pain. In medicine, researchers take a certain point that the brain is the control center of CSU, but in previous experiments, the researchers found that cerebellum also had a certain effect on CSU. In order to find out the influence of CSU in the brain and cerebellum, we collected the brain resting-state fMRI data from 40 healthy controls and 32 CSU patients and used DPABI to preprocess. We calculated the entropy values of five scales by using multiscale entropy (MSE) and the average entropy values of two groups' BOLD signals; 15 regions with significant differences were found which not only had a more detailed impact in the brain but also had an impact in the cerebellum, such as precentral gyrus, lenticular putamen, and vermis of cerebellum. In addition, we found that compared with the healthy controls, the entropy values of CSU patients showed two trends which need further study. The advantage of our experiment is that the multiscale entropy value is used to get more influence regions of CSU in the brain and cerebellum. The results of this paper may provide some help for the pathological study of CSU.
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http://dx.doi.org/10.1155/2021/6691356DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8172304PMC
May 2021

miR-1258 Attenuates Tumorigenesis Through Targeting E2F1 to Inhibit and Transcription in Glioblastoma.

Front Oncol 2021 17;11:671144. Epub 2021 May 17.

Department of Physiology, School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing, China.

MicroRNAs are a group of endogenous small non-coding RNAs commonly dysregulated in tumorigenesis, including glioblastoma (GBM), the most malignant brain tumor with rapid proliferation, diffuse invasion, and therapeutic resistance. Accumulating evidence has manifested that miR-1258 exerts an inhibitory role in many human cancers. However, the expression pattern of miR-1258 and its potential function in GBM tumorigenesis remain unclear. In this study, we reported that miR-1258 expression decreased with the ascending pathological grade of glioma, which indicated an unfavorable prognosis of patients. Functional assays revealed an inhibitory effect of miR-1258 on malignant proliferation, therapeutic resistance, migration, and invasion of GBM . Moreover, xenograft models also suggested a repression effect of miR-1258 on gliomagenesis. Mechanistically, miR-1258 directly targeted E2F1 in 3'-untranslated regions and attenuated E2F1-mediated downstream gene and transcriptions. Furthermore, restoration of E2F1 expression in GBM cells effectively rescued the tumor-suppressive effect of miR-1258. Our studies illustrated that miR-1258 functioned as a tumor suppressor in GBM by directly targeting E2F1, subsequently inhibiting and transcriptions, which contributed to new potential targets for GBM therapy and other E2F1-driven cancers.
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http://dx.doi.org/10.3389/fonc.2021.671144DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8166228PMC
May 2021

Amphiphilicity-adaptable graphene quantum dots to stabilize pH-responsive pickering emulsions at a very low concentration.

J Colloid Interface Sci 2021 May 21;601:106-113. Epub 2021 May 21.

Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, TX 77843, USA. Electronic address:

Hypothesis: Stimuli-responsive Pickering emulsions have attracted considerable interest due to their widespread potential applications. Especially pH-responsive behavior could be easily implemented. In this work, we reported a pH-responsive Pickering emulsion based on amphiphilic graphene quantum dots at a low concentration which shows a great potential from the environmental and economic perspective. The stimuli responsive properties would make the smart Pickering emulsifiers recyclable and reusable.

Experiments: The amphiphilic-adaptable graphene quantum dots functionalized by alkyl groups (C-GQDs) were synthesized by a facile one-step pyrolysis method. The pH-responsive emulsion performances were investigated, and the mechanism of pH-responsive of C-GQDs was studied by dynamic light scattering.

Findings: The amphiphilicity of C-GQDs could be acquired controllably and effectively by this facile one-step pyrolysis method, which are able to stabilize Pickering emulsion at a very low concentration (0.001%). The amphiphilicity of C-GQDs are capable of changing in response to environmental stimuli. When the pH value of aqueous solution adjusts to 2, these C-GQDs aggregate in contrast to their stability in neutral condition due to the alternation of surface charges. The pH-responsive aggregation/ dispersion behavior of C-GQDs allows us to tune the interactions between oil-in-water emulsion droplets without introduction of destabilization agents. This will provide huge economic benefits in industrial applications in the future.
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http://dx.doi.org/10.1016/j.jcis.2021.05.104DOI Listing
May 2021

Progress in Borneol Intervention for Ischemic Stroke: A Systematic Review.

Front Pharmacol 2021 4;12:606682. Epub 2021 May 4.

College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Borneol is a terpene and bicyclic organic compound that can be extracted from plants or chemically synthesized. As an important component of proprietary Chinese medicine for the treatment of stroke, its neuroprotective effects have been confirmed in many experiments. Unfortunately, there is no systematic review of these studies. This study aimed to systematically examine the neuroprotective effects of borneol in the cascade reaction of experimental ischemic stroke at different periods. Articles on animal experiments and cell-based research on the actions of borneol against ischemic stroke in the past 20°years were collected from Google Scholar, Web of Science, PubMed, ScienceDirect, China National Knowledge Infrastructure (CNKI), and other biomedical databases. Meta-analysis was performed on key indicators experiments. After sorting the articles, we focused on the neuroprotective effects and mechanism of action of borneol at different stages of cerebral ischemia. Borneol is effective in the prevention and treatment of nerve injury in ischemic stroke. Its mechanisms of action include improvement of cerebral blood flow, inhibition of neuronal excitotoxicity, blocking of Ca overload, and resistance to reactive oxygen species injury in the acute ischemic stage. In the subacute ischemic stage, borneol may antagonize blood-brain barrier injury, intervene in inflammatory reactions, and prevent neuron excessive death. In the late stage, borneol promotes neurogenesis and angiogenesis in the treatment of ischemic stroke. Borneol prevents neuronal injury after cerebral ischemia via multiple action mechanisms, and it can mobilize endogenous nutritional factors to hasten repair and regeneration of brain tissue. Because the neuroprotective effects of borneol are mediated by various therapeutic factors, deficiency caused by a single-target drug is avoided. Besides, borneol promotes other drugs to pass through the blood-brain barrier to exert synergistic therapeutic effects.
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http://dx.doi.org/10.3389/fphar.2021.606682DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129537PMC
May 2021

DNA-based microparticle tension sensors (µTS) for measuring cell mechanics in non-planar geometries and for high-throughput quantification.

Angew Chem Int Ed Engl 2021 May 12. Epub 2021 May 12.

Emory University, Chemistry, 1515 Dickey Dr., 30322, Atlanta, UNITED STATES.

Mechanotransduction, the interplay between physical and chemical signaling, plays vital roles in many biological processes ranging from cell differentiation to metastasis. The state-of-the-art techniques to quantify cell forces employ deformable polymer films or molecular probes tethered to glass substrates. These types of flat substrates limit applications in investigating mechanotransduction on non-planar geometries where physiological activities such as phagocytosis and immunological synapse formation mostly occur. A second challenge is the low throughput of microscopy readout which limits the application of current assays in fundamental and clinical research. We address these challenges by developing a DNA-based microparticle tension sensor (µTS), which features a spherical surface and thus allows for investigation of mechanical events at curved interfaces or within groups of cells in suspension. Importantly, the micron-scale of µTS enables flow cytometry readout, which is rapid and high throughput. To demonstrate the scope of µTS, we applied the method to map and measure T-cell receptor (TCR) forces and platelet integrin forces at 12 and 56 pN thresholds. Furthermore, we quantified the inhibition efficiency of two anti-platelet drugs providing a proof-of-concept demonstration of µTS to screen drugs that modulate cellular mechanics.
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http://dx.doi.org/10.1002/anie.202102206DOI Listing
May 2021

Neuroprotective Effect for Cerebral Ischemia by Natural Products: A Review.

Front Pharmacol 2021 22;12:607412. Epub 2021 Apr 22.

Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.

Natural products have a significant role in the prevention of disease and boosting of health in humans and animals. Stroke is a disease with high prevalence and incidence, the pathogenesis is a complex cascade reaction. In recent years, it's reported that a vast number of natural products have demonstrated beneficial effects on stroke worldwide. Natural products have been discovered to modulate activities with multiple targets and signaling pathways to exert neuroprotection via direct or indirect effects on enzymes, such as kinases, regulatory receptors, and proteins. This review provides a comprehensive summary of the established pharmacological effects and multiple target mechanisms of natural products for cerebral ischemic injury and preclinical models, and their potential neuro-therapeutic applications. In addition, the biological activity of natural products is closely related to their structure, and the structure-activity relationship of most natural products in neuroprotection is lacking, which should be further explored in future. Overall, we stress on natural products for their role in neuroprotection, and this wide band of pharmacological or biological activities has made them suitable candidates for the treatment of stroke.
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http://dx.doi.org/10.3389/fphar.2021.607412DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8102015PMC
April 2021

A two-miRNA signature of upregulated miR-185-5p and miR-362-5p as a blood biomarker for breast cancer.

Pathol Res Pract 2021 Jun 29;222:153458. Epub 2021 Apr 29.

Department of Breast Surgery, General Surgery, Qilu Hospital of Shandong University, 107 West Wenhua Road, Jinan, 250012, Shandong, People's Republic of China. Electronic address:

Background: Differentially expressed microRNAs (miRNAs) in the blood of breast cancer patients may serve as diagnostic biomarkers.

Methods: In this study, miRNA microarray of the blood of breast cancer patients and healthy controls was performed. Candidate differentially expressed miRNAs were further verified by real-time polymerase chain reaction in 68 breast cancer patients and 13 healthy controls.

Results: Six upregulated blood miRNAs (miR-26b-5p, miR-106b-5p, miR-142-3p, miR-142-5p, miR-185-5p, and miR-362-5p) were identified in breast cancer patients. These six miRNAs could discriminate breast cancer patients from healthy controls, with areas under the receiver operating characteristic curve (AUCs) of 0.8891, 0.8158, 0.8529, 0.8507, 0.9050, and 0.9333, respectively. Bioinformatic analysis showed that the six miRNAs were potentially involved in numerous cancer-related pathways, including the mitogen-activated protein kinase signaling pathway, nuclear factor-kappa B signaling pathway, and the transforming growth factor-beta signaling pathway. Importantly, two miRNAs (miR-185-5p and miR-362-5p) were used to construct a two-miRNA panel by logistic regression. The two-miRNA panel displayed a better diagnostic performance than each of the miRNAs alone, with a higher AUC (0.957), sensitivity (92.65 %), and specificity (92.31 %). Additionally, the high expression of the six miRNAs or the two-miRNA panel was associated with poor prognosis of breast cancer.

Conclusions: We identified six upregulated miRNAs and a two-miRNA panel in the blood as potential biomarkers for the diagnosis and prognosis of breast cancer.
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http://dx.doi.org/10.1016/j.prp.2021.153458DOI Listing
June 2021

Treatment of Degenerative Lumbar Scoliosis with Oblique Lumbar Interbody Fusion in Conjunction with Unilateral Pedicle Screw Fixation via the Wiltse Approach.

Orthop Surg 2021 May 4. Epub 2021 May 4.

Department of Orthopedics, General Hospital of Ningxia Medical University, Yinchuan, China.

Objective: To evaluate the clinical outcomes of oblique lumbar interbody fusion (OLIF) in conjunction with unilateral pedicle screw fixation (UPSF) via the Wiltse approach in treating degenerative lumbar scoliosis (DLS).

Methods: The article is a retrospective analysis. Twelve patients with DLS who underwent combined OLIF and UPSF between July 2017 and December 2018 were included. The study included 2 male and 10 female patients, with a mean age at the time of the operation of 67.2 ± 9.1 years. The surgical characteristics and complications were evaluated. The clinical and radiological data such as the correction of deformity, coronal and sagittal profile were analyzed.

Results: The mean follow-up time of the study was 26.8 ± 1.8 months. At the final follow-up, all patients who underwent combined OLIF and UPSF achieved statistically significant improvements in coronal Cobb angle (from 19.6° ± 4.8° to 6.9° ± 3.8°, P < 0.01), distance between the C7 plumb line and central sacral vertebral line (from 2.5 ± 1.7 cm to 0.9 ± 0.6 cm, P < 0.01), sagittal vertebral axis (from 4.3 ± 4.3 cm to 1.5 ± 1.0 cm, P = 0.03), lumbar lordosis (from 29.4° ± 8.6° to 40.8° ± 5.8°, P < 0.01), pelvic tilt (from 27.6° ± 10.8° to 18.3° ± 7.0°, P < 0.01), pelvic incidence-lumbar lordosis mismatch (from 23.3° ± 10.5° to 11.9° ± 8.4°, P < 0.01), and cross-sectional area of the dural sac (from 87.33 ± 39.41 mm to 124.70 ± 39.26 mm , P < 0.01). The visual analogue score for back and leg pain and Oswestry Disability Index of all patients significantly improved postoperatively (P < 0.01). One case of lumbar plexus injury was found after surgery. During the follow-up period, one patient had cage subsidence. A fusion rate of 100% and good positioning of the pedicle screws were achieved in all patients at the final follow-up.

Conclusion: OLIF in conjunction with UPSF is a safe and effective minimally invasive procedure for correcting both coronal and sagittal deformities, as it results in an improved quality of life in patients with DLS.
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http://dx.doi.org/10.1111/os.12960DOI Listing
May 2021

Sanguinarine Attenuates Collagen-Induced Platelet Activation and Thrombus Formation.

Biomedicines 2021 Apr 21;9(5). Epub 2021 Apr 21.

Department of Pharmacology, School of Basic Medicine, Tongji Medical College of Huazhong, University of Science and Technology, 13 Hangkong Road, Wuhan 430030, China.

Sanguinarine, a benzophenanthridine alkaloid, has been described to have an antiplatelet activity. However, its antithrombotic effect and the mechanism of platelet inhibition have not thoroughly been explored. The current study found that sanguinarine had an inhibitory effect on thrombus formation. This inhibitory effect was quite evident both in the flow-chamber assays as well as in a murine model of FeCl-induced carotid artery thrombosis. Further investigations also revealed that sanguinarine inhibited the collagen-induced human platelet aggregation and granule release. At the same time, it also prevented platelet spreading and adhesion to immobilized fibrinogen. The molecular mechanisms of its antiplatelet activity were found to be as follows: 1. Reduced phosphorylation of the downstream signaling pathways in collagen specific receptor GPVI (Syk-PLCγ2 and PI3K-Akt-GSK3β); 2. Inhibition of collagen-induced increase in the intracellular Ca concentration ([Ca]i); 3. Inhibition of integrin αIIbβ3 outside-in signaling via reducing β3 and Src (Tyr-416) phosphorylation. It can be concluded that sanguinarine inhibits collagen-induced platelet activation and reduces thrombus formation. This effect is mediated via inhibiting the phosphorylation of multiple components in the GPVI signaling pathway. Current data also indicate that sanguinarine can be of some clinical value to treat cardiovascular diseases involving an excess of platelet activation.
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http://dx.doi.org/10.3390/biomedicines9050444DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8142988PMC
April 2021

Homoharringtonine inhibited breast cancer cells growth via miR-18a-3p/AKT/mTOR signaling pathway.

Int J Biol Sci 2021 2;17(4):995-1009. Epub 2021 Mar 2.

The General Hospital of Ningxia Medical University, Biochip Research Center, Yinchuan, 750001, China.

Homoharringtonine (HHT), a natural alkaloid derived from the , exhibited its anti-cancer effects in hematological malignancies clinically. However, its pesticide effects and mechanisms in treating solid tumors remain unclear. In this study, we found that HHT was capable of inhibiting tumor growth after 5-days treatment of breast cancer cells, MCF-7, . Furthemore, HHT also significantly inhibited the cancer cell growth and induced cell apoptosis . miRNA sequencing proved miR-18a-3p was noticeably downregulated in the cells after HHT treatment. Moreover, downregulating miR-18a-3p increased HHT-induced cell apoptosis; our data supported that HHT suppressed miR-18a-3p expression and inhibited tumorigenesis might via AKT-mTOR signaling pathway. In conclusion: our study proved that HHT suppressed breast cancer cell growth and promoted apoptosis mediated by regulating of the miR-18a-3p-AKT-mTOR signaling pathway, HHT may be a promising antitumor agent in breast cancer treatment.
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http://dx.doi.org/10.7150/ijbs.44907DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8040299PMC
March 2021

[Retracted] Regulation of the cell cycle and PI3K/Akt/mTOR signaling pathway by tanshinone I in human breast cancer cell lines.

Mol Med Rep 2021 06 13;23(6). Epub 2021 Apr 13.

First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210046, P.R. China.

Following the publication of the above paper, a concerned reader drew to the Editor's attention that Fig. 5 contained apparent anomalies, including unexpectedly similar-looking cells and repeated patternings of the cells in terms of their layout/arrangement within the data panels. After having conducted an independent investigation in the Editorial Office, the Editor of has determined that the above paper should be retracted from the Journal on account of a lack of confidence regarding the authenticity of the data. The authors were asked for an explanation to account for these concerns, but the Editorial Office never received any reply. The Editor regrets any inconvenience that has been caused to the readership of the Journal. [the original article was published in 11: 931‑939, 2015; DOI: 10.3892/mmr.2014.2819].
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http://dx.doi.org/10.3892/mmr.2021.12080DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8060802PMC
June 2021

Biogenesis, physiological functions and potential applications of extracellular vesicles in substance use disorders.

Cell Mol Life Sci 2021 Apr 5. Epub 2021 Apr 5.

Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, 68198-5880, USA.

Substance use disorder (SUD) is a growing health problem that affects several millions of people worldwide, resulting in negative socioeconomic impacts and increased health care costs. Emerging evidence suggests that extracellular vesicles (EVs) play a crucial role in SUD pathogenesis. EVs, including exosomes and microvesicles, are membrane-encapsulated particles that are released into the extracellular space by most types of cells. EVs are important players in mediating cell-to-cell communication through transfer of cargo such as proteins, lipids and nucleic acids. The EV cargo can alter the status of recipient cells, thereby contributing to both physiological and pathological processes; some of these play critical roles in SUD. Although the functions of EVs under several pathological conditions have been extensively reviewed, EV functions and potential applications in SUD remain less studied. In this review, we provide an overview of the current knowledge of the role of EVs in SUD, including alcohol, cocaine, heroin, marijuana, nicotine and opiate abuse. The review will focus on the biogenesis and cargo composition of EVs as well as the potential use of EVs as biomarkers of SUD or therapeutic targets in SUD.
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http://dx.doi.org/10.1007/s00018-021-03824-8DOI Listing
April 2021

DNA Tension Probes to Map the Transient Piconewton Receptor Forces by Immune Cells.

J Vis Exp 2021 03 20(169). Epub 2021 Mar 20.

Department of Chemistry, Emory University; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University;

Mechanical forces transmitted at the junction between two neighboring cells and at the junction between cells and the extracellular matrix are critical for regulating many processes ranging from development to immunology. Therefore, developing the tools to study these forces at the molecular scale is critical. Our group developed a suite of molecular tension sensors to quantify and visualize the forces generated by cells and transmitted to specific ligands. The most sensitive class of molecular tension sensors are comprised of nucleic acid stem-loop hairpins. These sensors use fluorophore-quencher pairs to report on the mechanical extension and unfolding of DNA hairpins under force. One challenge with DNA hairpin tension sensors is that they are reversible with rapid hairpin refolding upon termination of the tension and thus transient forces are difficult to record. In this article, we describe the protocols for preparing DNA tension sensors that can be "locked" and prevented from refolding to enable "storing" of mechanical information. This allows for the recording of highly transient piconewton forces, which can be subsequently "erased" by the addition of complementary nucleic acids that remove the lock. This ability to toggle between real-time tension mapping and mechanical information storing reveals weak, short-lived, and less abundant forces, that are commonly employed by T cells as part of their immune functions.
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http://dx.doi.org/10.3791/62348DOI Listing
March 2021

Combination of urine and faeces metabolomics to reveal the intervention mechanism of Polygala tenuifolia compatibility with Magnolia officinalis on gastrointestinal motility disorders.

J Pharm Pharmacol 2021 Mar;73(2):247-262

School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China.

Objectives: To explore the intervention mechanism of combining Polygala tenuifolia (PT) with Magnolia officinalis (MO) on gastrointestinal motility disorders caused by PT.

Methods: Urine and faeces of rats were collected; the effects of PT and MO on the gastric emptying and small intestine advancing rates in mice were analysed via ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) to determine the potential metabolites. Changes in the metabolic profiles of the urine and faeces were revealed by untargeted metabolomics, followed by multivariate statistical analysis. The integration of urine and faeces was applied to reveal the intervention mechanism of PT-MO on PT-induced disorders.

Key Findings: PT + MO (1:2) improved the gastrointestinal function in mice suffering from PT-induced gastrointestinal motility disorder. Metabolomics indicated that the PT-MO mechanism was mainly associated with the regulations of 17 and 12 metabolites and 11 and 10 pathways in urine and faeces, respectively. The common metabolic pathways were those of tyrosine, purine, tricarboxylic acid cycle, pyruvate and gluconeogenesis, which were responsible for the PT-MO intervention mechanism.

Conclusions: The PT-MO (1:2) couple mechanism mitigated the PT-induced disorders, which were related to the energy, amino acid and fatty metabolisms.
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http://dx.doi.org/10.1093/jpp/rgaa022DOI Listing
March 2021

Lymphopenia in Esophageal Cancer: What Have We Learned?

Front Oncol 2021 11;11:625963. Epub 2021 Mar 11.

Department of Radiation Oncology, General Hospital of Ningxia Medical University, Yinchuan, China.

Lymphopenia caused by disease or treatment is frequent in patients with cancer, which seriously affects the prognosis of these patients. Immune checkpoint inhibitors (ICIs) have garnered attention as one of the most promising strategies for the treatment of esophageal cancer (EC). The status of the immune system, such as, the lymphocyte count, is now considered to be an important biomarker for ICI treatments. Recognition of the significant impact of the lymphocyte count on the survival of patients with EC in the era of immunotherapy has revived interest in understanding the causes of lymphopenia and in developing strategies to predict, prevent and eliminate the adverse effect of lymphopenia. Here, we review what we have learned about lymphopenia in EC, including the prognostic and predictive value of lymphopenia in patients with EC, the predictors of lymphopenia, and the strategies to ameliorate the effect of lymphopenia in patients with EC.
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http://dx.doi.org/10.3389/fonc.2021.625963DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8006429PMC
March 2021

Acupuncture for treating tic disorders in children: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 Mar;100(12):e24860

Department of Pediatrics, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Xiqing.

Background: Tic disorders (TDs) are a group of neurodevelopmental disorders in children, while pharmacotherapy is often associated with various side effects and has limited clinical effects for some patients, thus significantly affecting patients' quality of life. Studies have found acupuncture shows certain advantages in the treatment of TDs. However, there is no high level of evidence evaluating the effectiveness and safety of acupuncture for children with TDs.

Methods: Each data of acupuncture for treating TDs will be searched. We will search for related English and Chinese databases. The time is limited from inception until November 2020. The primary outcome is the reduction rate (amount) of tic severity using related scales or methods, and the secondary outcomes include recurrence rate and adverse events. The risk of bias will be assessed, and the RevMan5.3 and Stata14.0 will be performed for meta-analysis. Finally, we will assess the level of the resulting evidence.

Results: The results of the study will synthesize the current evidence and be published in peer-reviewed journals.

Conclusions: This research aims to provide convincing evidence of the effectiveness and safety of acupuncture for treating TDs in children.

Inplasy Registration Number: INPLASY2020110050.
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http://dx.doi.org/10.1097/MD.0000000000024860DOI Listing
March 2021

Chi-miR-370-3p regulates hair follicle morphogenesis of Inner Mongolian cashmere goats.

G3 (Bethesda) 2021 May;11(5)

College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, Inner Mongolia, China.

MicroRNAs (miRNAs), a class of 22 nucleotide (nt) noncoding RNAs, negatively regulate mRNA posttranscriptional modification in various biological processes. Morphogenesis of skin hair follicles in cashmere goats is a dynamic process involving many key signaling molecules, but the associated cellular biological mechanisms induced by these key signaling molecules have not been reported. In this study, differential expression, bioinformatics, and Gene Ontology/Kyoto Encyclopedia of Genes and Genomes enrichment analyses were performed on miRNA expression profiles of Inner Mongolian cashmere goats at 45, 55, and 65 days during the fetal period, and chi-miR-370-3p was identified and investigated further. Real-time fluorescence quantification (qRT-PCR), dual luciferase reporting, and Western blotting results showed that transforming growth factor beta receptor 2 (TGF-βR2) and fibroblast growth factor receptor 2 (FGFR2) were the target genes of chi-miR-370-3p. Chi-miR-370-3p also regulated the expression of TGF-βR2 and FGFR2 at mRNA and protein levels in epithelial cells and dermal fibroblasts. DNA staining, Cell Counting Kit-8, and fluorescein-labelled Annexin V results showed that chi-miR-370-3p inhibited the proliferation of epithelial cells and fibroblasts but had no effect on apoptosis. Cell scratch test results showed that chi-miR-370-3p promoted the migration of epithelial cells and fibroblasts. Chi-miR-370-3p inhibits the proliferation of epithelial cells and fibroblasts by targeting TGF-βR2 and FGFR2, thereby improving cell migration ability and ultimately regulating the fate of epithelial cells and dermal fibroblasts to develop the placode and dermal condensate, inducing hair follicle morphogenesis.
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http://dx.doi.org/10.1093/g3journal/jkab091DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8104936PMC
May 2021

-Borneol Exerted the Neuroprotective Effect by Promoting Angiogenesis Coupled With Neurogenesis via Ang1-VEGF-BDNF Pathway.

Front Pharmacol 2021 5;12:641894. Epub 2021 Mar 5.

Department of Pathology, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.

At present, Stroke is still one of the leading causes of population death worldwide and leads to disability. Traditional Chinese medicine plays an important role in the prevention or treatment of stroke. -borneol, a traditional Chinese medicine, has been used in China to treat stroke for thousands of years. However, its mechanism of action is unclear. After cerebral ischemia, promoting angiogenesis after cerebral ischemia and providing nutrition for the infarct area is an important strategy to improve the damage in the ischemic area, but it is also essential to promote neurogenesis and replenish new neurons. Here, our research shows that -borneol can significantly improve the neurological deficits of pMCAO model rats, reduce cerebral infarction, and improve the pathological damage of cerebral ischemia. and significantly increase serum level of Ang-1 and VEGF, and significantly decrease level of ACE and Tie2 to promote angiogenesis. PCR and WB showed the same results. Immunohistochemistry also showed that borneol can increase the number of CD34 positive cells, further verifying that borneol can play a neuroprotective effect by promoting angiogenesis after cerebral ischemia injury. In addition, borneol can significantly promote the expression level of VEGF, BDNF and inhibit the expression levels of TGF-β1 and MMP9 to promote neurogenesis. The above suggests that borneol can promote angiogenesis coupled neurogenesis by regulating Ang1-VEGF-BDNF to play a neuroprotective effect. Molecular docking also shows that borneol has a very high binding rate with the above target, which further confirmed the target of borneol to improve cerebral ischemic injury. These results provide strong evidence for the treatment of cerebral ischemia with borneol and provide reference for future research.
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http://dx.doi.org/10.3389/fphar.2021.641894DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7973462PMC
March 2021

XT-considered multiple backup paths and resources shared protection scheme based on ring covers.

Opt Express 2021 Mar;29(5):6737-6755

With the rapid development of space division multiplexing (SDM) and flexible grid technology, the problem of resource allocation in optical network becomes much more complicated. Although there emerge a substantial number of works about link protection or restoration in SDM-EONs mesh networks, the topic of survivability is dug deeper in this work. It is acknowledged that protection schemes based on ring covers bring the advantages of shorter restoration time and lower costs. However, the RSCA problem in SDM-EON for link protection based on ring covers has rarely been investigated. To enhance the survivability of SDM-EON and make the best use of ring covers, we initially select a set of rings for the protection of all the links in a network topology according to constrains, aiming at taking full advantages of network resources. After that, we propose an algorithm to recover the traffic which will break off under link failures, basing on the set of rings. At the end of this protection scheme, we arrange the resources fulfilling the constrains of spectrum contiguity and core continuity constrains by using several different algorithms considering physical impairment. Based on the allocation in both spacial and spectral dimensions, our algorithms achieve better results of survivability. According to the simulations conducted for the evaluation of the proposed algorithms, our algorithms manage to recover at least 72.8% of traffic when the traffic request number is set to be 1000.
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http://dx.doi.org/10.1364/OE.400042DOI Listing
March 2021

Chinese herbal compounds containing scorpion in the treatment of epilepsy: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2021 Mar;100(10):e25134

Department of Pediatrics, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Xiqing.

Background: Epilepsy is 1 of the common neurodevelopmental diseases. It can affect about 0.5% to 1.0% of the population regardless of their race and social class. Despite the development of a wide range of treatments, there remaining about one-third of patients still experience seizures. Chinese herbal compounds containing scorpion (CHCCS) have shown an outstanding curative effect on nerve protection and epilepsy. But there's no study to assess its clinical efficacy and safety.

Methods: Each data of CHCCS in treating epilepsy from related English and Chinese databases will be searched. The primary outcome is the efficacy of the CHCCS on epilepsy. And the secondary outcomes include recurrence rate and side effects. The risk of bias will be assessed, and RevMan5.3 and Stata14.0 will be performed for meta-analysis. Finally, we will assess the level of the resulting evidence.

Results: The results of the study will be combined with current evidence and published in a peer-reviewed journal.

Conclusions: This study will specifically investigate the effectiveness and safety of CHCCS in treating epilepsy.

Inplasy Registration Number: INPLASY202120056.
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http://dx.doi.org/10.1097/MD.0000000000025134DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969296PMC
March 2021

Safety and efficacy of long-term mild hypothermia for severe traumatic brain injury with refractory intracranial hypertension (LTH-1): A multicenter randomized controlled trial.

EClinicalMedicine 2021 Feb 28;32:100732. Epub 2021 Jan 28.

Head Trauma Center, Department of Neurosurgery, Renji Hospital, Shanghai Jiao Tong University/School of Medicine, Shanghai Institute of Head Trauma, Shanghai, China.

Background: Therapeutic hypothermia may need prolonged duration for the patients with severe traumatic brain injury (sTBI).

Methods: The Long-Term Hypothermia trial was a prospective, multicenter, randomized, controlled clinical trial to examine the safety and efficacy in adults with sTBI. Eligible patients were 18-65, Glasgow Coma Scale score at 4 to 8, and initial intracranial pressure (ICP) ≥ 25 mm Hg, randomly assigned to the long-term mild hypothermia group (34-35 °C for 5 days) or normothermia group at 37 °C. The primary outcome was the Glasgow outcome scale (GOS) at 6 months. Secondary outcomes included ICP control, complications and laboratory findings, the length of ICU and hospital stay, and GOS at 6 months in patients with initial ICP ≥ 30 mm Hg. This trial is registered with ClinicalTrials.gov, NCT01886222.

Findings: 302 patients were enrolled from June 25, 2013, to December 31, 2018, with 6 months follow-up in 14 hospitals, 156 in hypothermia group and 146 in normothermia group. There was no difference in favorable outcome (OR 1·55, 95%CI 0·91-2·64;  = 0·105) and in mortality ( = 0·111) between groups. In patients with an initial ICP ≥ 30 mm Hg, hypothermic treatment significantly increased favorable outcome over normothermia group (60·82%, 42·71%, respectively; OR 1·861, 95%CI 1·031-3·361;  = 0·039). Long-term mild hypothermia did not increase the incidences of complications.

Interpretation: Long-term mild hypothermia did not improve the neurological outcomes. However, it may be a potential option in sTBI patients with initial ICP ≥ 30 mm Hg.

Funding: : Shanghai municipal government and Shanghai Jiao Tong University/School of Medicine.
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http://dx.doi.org/10.1016/j.eclinm.2021.100732DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7910713PMC
February 2021

Hypermethylation of heparanase 2 promotes colorectal cancer proliferation and is associated with poor prognosis.

J Transl Med 2021 03 5;19(1):98. Epub 2021 Mar 5.

Research Center for Clinical Oncology, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University, 42 Baiziting, Nanjing, 210000, Jiangsu, People's Republic of China.

Background: The epigenetic abnormality of tumor-associated genes contributes to the pathogenesis of colorectal carcinoma (CRC). However, methylation in colorectal cancer is still poorly characterized.

Method: By integration of DNA methylation data from the GEO database and gene expression data from The Cancer Genome Atlas database, the aberrantly methylated genes involved in CRC tumorigenesis were identified. Subsequent in vitro experiments further validated their role in CRC.

Results: We performed integrative genomic analysis and identified HPSE2, a novel tumor suppressor gene that is frequently inactivated through promoter methylation in CRC. K-M survival analysis showed that hypermethylation-low expression of heparanase 2 (HPSE2) was related to poor patient prognosis. Overexpression of HPSE2 reduced cell proliferation in vivo and in vitro. HPSE2 could regulate the p53 signaling pathway to block the cell cycle in G1 phase.

Conclusion: HPSE2, a novel tumor suppressor gene that is frequently inactivated through promoter methylation in CRC. HPSE2 performs a tumor suppressive function by activating the p53/ p21 signaling cascade. The promoter hypermethylation of HPSE2 is a potential therapeutic target in patients with CRC, especially those with late-stage CRC.
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http://dx.doi.org/10.1186/s12967-021-02770-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7934273PMC
March 2021

Dl-3-n-Butylphthalide Reduces Cognitive Deficits and Alleviates Neuropathology in P301S Tau Transgenic Mice.

Front Neurosci 2021 10;15:620176. Epub 2021 Feb 10.

Tongji Medical College, Wuhan Union Hospital, Huazhong University of Science and Technology, Wuhan, China.

Alzheimer's disease (AD) is a destructive and burdensome neurodegenerative disease, one of the most common characteristics of which are neurofibrillary tangles (NFTs) that are composed of abnormal tau protein. Animal studies have suggested that dl-3-n-butylphthalide (dl-NBP) alleviates cognitive impairment in mouse models of APP/PS1 and SAMP8. However, the underlying mechanisms related to this remain unclear. In this study, we examined the effects of dl-NBP on learning and memory in P301S transgenic mice, which carry the human tau gene with the P301S mutation. We found that dl-NBP supplementation effectively improved behavioral deficits and rescued synaptic loss in P301S tau transgenic mice, compared with vehicle-treated P301S mice. Furthermore, we also found that it markedly inhibited the hyperphosphorylated tau at the Ser262 site and decreased the activity of MARK4, which was associated with tau at the Ser262 site. Finally, dl-NBP treatment exerted anti-inflammatory effects and reduced inflammatory responses in P301S mice. In conclusion, our results provide evidence that dl-NBP has a promising potential for the therapy of tauopathies, including AD.
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http://dx.doi.org/10.3389/fnins.2021.620176DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7902884PMC
February 2021

Down-regulation of estrogen-related receptor alpha (ERRα) inhibits gastric cancer cell migration and invasion and .

Aging (Albany NY) 2021 02 11;13(4):5845-5857. Epub 2021 Feb 11.

Department of Medical Oncology, Jiangsu Cancer Hospital and Jiangsu Institute of Cancer Research and The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing 210009, Jiangsu Province, China.

Objective: To investigate the correlation between estrogen-related receptor a (ERRα) expression level and gastric cancer (GC).

Methods: We collected GC and adjacent normal tissues from 50 patients. The parameters of the patients were summarized, and correlation with the expression level of ERRα was calculated. Downregulated ERRα using lentivirus was designed and transfected to SGC-7901 and MGC-803 cells. Cell migration, invasion and wound assays were conducted to determine the correlation between ERRα and capacity for cell migration and invasion. The expression level of the genes involved in epithelial-mesenchymal transition, including E-cadherin, γ-catenin, N-cadherin and vimentin, was determined via real-time or quantitative polymerase chain reaction(qPCR) and Western blot analysis.

Results: The expression of ERRα tends to be higher in GC tissues than in adjacent normal tissues. Analyses ofthe expression level of ERRα and patient parameters show that the ERRα level is significantly correlated with TNM staging and patient survival (<0.05). The downregulation of ERRα can inhibit cell invasion and migration, which was proven by Transwell and cell wound assays. The levels of E-cadherin and γ-catenin increased by conducting qPCR and Western blot analysis. Meanwhile, the levels of N-cadherin and vimentin decreased when ERRα expression was reduced.

Conclusion: ERRα is highly expressed in GC tissues and can promote the migration and invasion of cancer cells. It can be a potential marker for GC diagnosis.
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http://dx.doi.org/10.18632/aging.202508DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7950300PMC
February 2021

Chi-miR-130b-3p regulates Inner Mongolia cashmere goat skin hair follicles in fetuses by targeting Wnt family member 10A.

G3 (Bethesda) 2021 Jan;11(1)

Engineering Research Center for Goat Genetics and Breeding, Hohhot 010018, Inner Mongolia Autonomous Region, China.

The development of hair follicles (HFs) is dependent on interactions between epithelial cells and dermal fibroblasts, which may play an important role in maintaining the structure of HFs during their development and maturation. Wnt family member 10 (WNT10A) is a hub gene during HF development and maturation that may regulate the proliferation of dermal fibroblasts and epithelial cells through microRNAs (miRNAs) and messenger RNAs (mRNAs) to maintain the structural stability of HFs. In the present study, we confirmed that WNT10A is the target gene of chi-miR-130b-3p by real-time quantitative PCR, western blotting, and a dual-luciferase reporter gene assay. We successfully cultured fetal epithelial cells and dermal fibroblasts using the tissue block attachment method, and Cell Counting Kit-8 (CCK8) results showed that chi-miR-130b-3p regulates epithelial cell and dermal fibroblast proliferation by targeting WNT10A.
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http://dx.doi.org/10.1093/g3journal/jkaa023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8022718PMC
January 2021

Compound Cocktail Inhibits Influenza Viral Pneumonia via Phospholipase Cγ1 Phosphorylation-Related Necroptosis and Partial Autophagy in Natural Killer Cells.

Planta Med 2021 Jun 5;87(7):538-549. Epub 2021 Feb 5.

Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.

Influenza viral infections are prone to global outbreaks and cause pneumonia in affected populations. High morbidity and mortality caused by pneumonia occur during an influenza pandemic. Antivirals or control of inflammation is the primary means of influenza treatment. A compound cocktail composed of arctiin, daidzein, glycyrrhizic acid, and liquiritin inhibited mouse pneumonia resulting from a PR8 viral infection and caused a weight gain after oral administration. Natural killer cell activating receptors, both Ly49D and Ly49H in the lungs, were increased in the treatment in mice. In H3N2 virus-infected natural killer-92MI cells, the cocktail treatment had different effects on phosphorylation sites of phospholipase C1 (PLC1) and killed infected cells through necroptosis or late apoptosis, in which RIP3 was increased and both caspase-3 and phosphorylated-JNK in the cells were downregulated. Acid phosphatase activity in viral-infected natural killer-92MI cells was induced by the compound cocktail treatment, which could be related to the p62 decrease in natural killer-92MI cells. In addition, an autophagic flux induction was observed in alveolar basal epithelial cells (A549). Protein p65, but not phosphorylated-p65, was significantly decreased by the treatment. Our results indicate that the compound cocktail strengthened the phosphorylation of PLC1-related necroptosis and partial autophagy in natural killer cells, which could yield an inhibitory effect on viral pneumonia in influenza.
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http://dx.doi.org/10.1055/a-1353-6672DOI Listing
June 2021

Predicting Intentions to Vaccinate against COVID-19 and Seasonal Flu: The Role of Consideration of Future and Immediate Consequences.

Authors:
Zexin Ma Rong Ma

Health Commun 2021 Feb 4:1-10. Epub 2021 Feb 4.

Department of Communication and Media Studies, College of Communication, Butler University.

Understanding the social-psychological determinants of the public's perceptions and intentions related to vaccination is key to promoting vaccination. The current study examines how individual differences in consideration of future and immediate consequences (CFC-F and CFC-I) impact risk perceptions of, and intentions to vaccinate against, COVID-19 and seasonal flu. A survey of 395 adults on Amazon Mechanical Turk during April and May of 2020 showed that CFC-F predicted vaccination intentions, whereas CFC-I did not. Moreover, CFC-F and CFC-I positively predicted affective risk perceptions, perceived susceptibility, and perceived severity of both COVID-19 and seasonal flu. Last, both CFC constructs had a positive indirect effect on vaccination intentions of COVID-19 and seasonal flu through increasing perceived severity of the corresponding disease. This study makes theoretical contributions to the CFC literature and offers valuable insights for the design of effective vaccine promotion messages.
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http://dx.doi.org/10.1080/10410236.2021.1877913DOI Listing
February 2021

A new immunotherapy strategy targeted CD30 in peripheral T-cell lymphomas: CAR-modified T-cell therapy based on CD30 mAb.

Cancer Gene Ther 2021 Jan 29. Epub 2021 Jan 29.

Research Center of Clinical Oncology, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & Nanjing Medical University Affiliated Cancer Hospital, Nanjing, 210009, P. R. China.

Chimeric antigen receptor T-cell immunotherapy (CAR-T) has shown remarkable efficacy in treating tumors of lymphopoietic origin. Herein, we demonstrate an effective CAR-T cell treatment for recurrent and malignant CD30-positive peripheral T-cell lymphomas (PTCL) has been demonstrated. The extracellular fragment gene sequences of CD30 were obtained from tumor tissues of PTCL patients and cloned into a plasmid vector to express the CD30 antigen. The CD30 targeting single-chain antibody fragment (scFv) was obtained from CD30-positive monoclonal hybridoma cells, which were obtained from CD30 antigen immunized mice. After a second-generation of CAR lentiviral construction, CD30 CAR T cells were produced and used to determine the cytotoxicity of this construct toward Karpas 299 cells. The results of CD30 CAR T-mediated cell lysis show that 9C11-2 CAR T cells could significantly promote the lysis of CD30-positive Karpas 299 cells in both LDH and real-time cell electronic sensing (RTCA) assays. In vivo data show that 9C11-2 CAR T cells effectively suppress the tumor growth in a Karpas 299 cell xenograft NCG mouse model. The CD30 CAR T cells exhibited an efficient cytotoxic effect after being co-cultured with the target cells and they also exhibited a significant tumor-inhibiting ability after being intravenously injected into PTCL xenograft tumors; these observations suggest that the new CD30 CAR-T cell may be a promising therapeutic candidate for cancer therapy.
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http://dx.doi.org/10.1038/s41417-021-00295-8DOI Listing
January 2021

lncRNA-Xist/miR-101-3p/KLF6/C/EBPα axis promotes TAM polarization to regulate cancer cell proliferation and migration.

Mol Ther Nucleic Acids 2021 Mar 10;23:536-551. Epub 2020 Dec 10.

Department of Pharmacology, School of Pharmacy, China Medical University, Shenyang City, 110122 Liaoning Province, China.

The phenotypic switch in tumor-associated macrophages (TAMs) mediates immunity escape of cancer. However, the underlying mechanisms in the TAM phenotypic switch have not been systematically elucidated. In this study, long noncoding RNA (lncRNA)-Xist, CCAAT/enhancer-binding protein (C/EBP)α, and Kruppel-like factor 6 (KLF6) were upregulated, whereas microRNA (miR)-101 was downregulated in M1 macrophages-type (M1). Knockdown of Xist or overexpression of miR-101 in M1 could induce M1-to-M2 macrophage-type (M2) conversion to promote cell proliferation and migration of breast and ovarian cancer by inhibiting C/EBPα and KLF6 expression. Furthermore, miR-101 could combine with both Xist and C/EBPα and KLF6 through the same microRNA response element (MRE) predicted by bioinformatics and verified by luciferase reporter assays. Moreover, we found that miR-101 knockdown restored the decreased M1 marker and the increased M2 marker expression and also reversed the promotion of proliferation and migration of human breast cancer cells (MCF-7) and human ovarian cancer (OV) cells caused by silencing Xist. Generally, the present study indicates that Xist could mediate macrophage polarization to affect cell proliferation and migration of breast and ovarian cancer by competing with miR-101 to regulate C/EBPα and KLF6 expression. The promotion of Xist expression in M1 macrophages and inhibition of miR-101 expression in M2 macrophages might play an important role in inhibiting breast and ovarian tumor proliferation and migration abilities.
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http://dx.doi.org/10.1016/j.omtn.2020.12.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7810606PMC
March 2021