Publications by authors named "Rong Huang"

919 Publications

New species and material of Hagloidea (Insecta, Ensifera) from the Yanliao biota of China.

Zookeys 2021 22;1033:183-190. Epub 2021 Apr 22.

College of Life Sciences, Capital Normal University, 105 Xisanhuanbeilu, Haidian District, Beijing, 100048, China Capital Normal University Beijing China.

A new species of Cyrtophyllitinae, Gu, Yue & Ren, is described from the Middle Jurassic Jiulongshan Formation, Daohugou Village, Inner Mongolia, China. The species is characterized by its ScA reaching the anterior wing margin at the level of the divergence of M+ CuA, distally branched RP, lengths of free CuA and free M equal, and numerous branches of CuA + CuPaα. A new fossil of Gu & Ren, 2012 is described which increases knowledge of its wing venation and indicates that should be assigned to the Tuphellidae.
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http://dx.doi.org/10.3897/zookeys.1033.63571DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8084857PMC
April 2021

The Cell-impermeable Ru(II) Polypyridyl Complex as a Potent Intracellular Photosensitizer under Visible Light Irradiation via Ion-Pairing with Suitable Lipophilic Counter-Anions.

Free Radic Biol Med 2021 May 3. Epub 2021 May 3.

State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, And University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing, 100085, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China; Joint Institute for Environmental Science, Research Center for Eco-Environmental Sciences and Hong Kong Baptist University, Hong Kong PR China. Electronic address:

Developing the cell-impermeable Ru(II) polypyridyl cationic complexes as effective photosensitizers (PS) which have high cellular uptake and photo-toxicity, but low dark toxicity, is quite challenging. Here we found that the highly reactive singlet oxygen (O) can be generated by the irradiation of a typical Ru(II) polypyridyl complex Ru(II)tris(tetramethylphenanthroline) ([Ru(TMP)]) under visible light irradiation by ESR with TEMPO (2,2,6,6-tetramethyl-4-piperidone-N-oxyl) as O probe. Effective cellular and nuclear delivery of cationic [Ru(TMP)] was achieved through our recently developed ion-pairing method, and 2,3,4,5-tetrachlorophenol (2,3,4,5-TeCP) was found to be the most effective among all chlorophenols tested. The accelerated cellular, especially nuclear uptake of [Ru(TMP)] results in the formation of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) and DNA strand breaks, caspase 3/7 activation and cell apoptosis in HeLa cells upon light irradiation. More importantly, compared with other traditional photosensitizers, [Ru(TMP)] showed significant photo-toxicity but low dark toxicity. Similar effects were observed when 2,3,4,5-TeCP was substituted by the currently clinically used anti-inflammatory drug flufenamic acid. This represents the first report that the cell-impermeable Ru(II) polypyridyl complex ion-paired with suitable lipophilic counter-anions functions as potent intracellular photosensitizer under visible light irradiation mainly via a O-mediated mechanism. These findings should provide new perspectives for future investigations on other metal complexes with similar characteristics as promising photosensitizers for potential photodynamic therapy.
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http://dx.doi.org/10.1016/j.freeradbiomed.2021.04.035DOI Listing
May 2021

The Implementation and Five-Year Evolution of a Childhood Healthy Weight Program: Making a Health Care-Community Partnership Work.

Child Obes 2021 May 3. Epub 2021 May 3.

School of Public Health, University of Texas Health Science Center, Dallas, TX, USA.

Sustained implementation of moderate to high-intensity interventions to treat childhood obesity meets many barriers. This report uses the Centers for Disease Control and Prevention's (CDC's) Replicating Effective Programs framework to describe and evaluate the implementation of a 5-year health care-community collaborative program. Interviews with program leadership provided information on setting, organizational culture, program creation and adaptation, and costs. Administrative data were used for number of sessions and their characteristics; referrals; and 2018-2019 participant enrollment, attendance, completion numbers, and completer outcomes. Preconditions for this program were high childhood obesity prevalence, and the complementary strengths of the health care organization (primary care treatment referral stream, population health orientation, alternative Medicaid funding) and the community organization (accessible space and time, staffing model, and organization mission). Preimplementation steps included collaborative design of a curriculum and allocation of administrative tasks. Implementation led to simultaneous deployment in as many as 17 community locations, with sessions offered free to families weekday evenings or weekends, delivered in English or Spanish. From 2018 to 2019, 2746 children were referred from nearly 300 providers, 832 (30.3%) enrolled, and 553 (66.3%) attended at least once, with 392 (70.8% of attenders and 47.1% of enrolled) completing the program. Outcomes in completers included improvement in %BMI [-2.34 (standard deviation, SD 4.19)] and Progressive Aerobic Cardiovascular Endurance Run (PACER) laps [2.46 (SD 4.74)],  < 0.0001 for both. Evolution, including in referral process, Spanish program material and delivery, and range of ages, occurred continuously rather than at discrete intervals. Major system disruptions also affected the implementation. Maintenance of the program relied on the health care organization's administrative team and the collaboration with the community organization. This program's collaboration across organizations and ongoing adaptation were necessary to build and sustain a program with broad reach and positive health outcomes. The lessons learned may be helpful for other programs.
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http://dx.doi.org/10.1089/chi.2021.0010DOI Listing
May 2021

Flexible integrated sensing platform for monitoring wound temperature and predicting infection.

Microb Biotechnol 2021 May 4. Epub 2021 May 4.

Department of Burn and Plastic Surgery, Second Affiliated Hospital of Air Force Military Medical University, Xi'an, 710000, China.

Wound infection is a challenging clinical problem that imposes substantial economic and psychological burdens on patients. However, the wound covered by a dressing is in an 'unknown' state. Recently, researchers have focused on understanding the condition of the wound without removing the dressing. Here, we presented a flexible integrated sensing platform (FISP) that can monitor multiple indicators, including local temperature. The platform consists of a flexible sensor chip (FSC), a controlled printed circuit board (CPCB) and a customized application installed on a smartphone that can receive and display data from the sensor chip through Bluetooth Low Energy 4.0 (BLE4.0) and upload real-time wound information. This device exhibits satisfactory measurement accuracy, stability, durability, skin compliance and biocompatibility. It was applied to infected wounds on the back of rabbits to reveal the temperature changes characteristic of wounds infected with different bacteria, and this information was compared with the changes in the core body temperature of animals. We found differences in the temperature among wounds infected with different pathogens and the temperature of the wound infection occurred earlier than the change in anal temperature. The combined application of the FISP and dressings might help identify the 'unknown' state of wounds in the clinic.
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http://dx.doi.org/10.1111/1751-7915.13821DOI Listing
May 2021

An integrated method to produce fermented liquid feed and biologically modified biochar as cadmium adsorbents using corn stalks.

Waste Manag 2021 Apr 29;127:112-120. Epub 2021 Apr 29.

College of Resources, Sichuan Agricultural University, Chengdu 611130, China. Electronic address:

The recycling of agricultural waste is a global challenge to the sustainable development of agriculture. By using corn stalks, we studied the feasibility of combining anaerobic fermentation and pyrolysis processes to produce both fermentated liquid feed and biologically modified biocharas cadmium adsorbents. Anaerobic ensiling enhanced the biodegradation of corn stalks by increasing crude protein and reducing fiber contents. After 24-h anaerobic fermentation, corn stalks silage was decomposed into the liquid filtrate and non-fermented residue. Fermented liquid feed (FLF) was prepared by storing feed and liquid filtrate (1:4.0, wt/wt) in a closed tank at 20 °C for 4 days, which showed desired properties (pH < 4.5, lactic acid bacteria greater than 9.0 lg cfu g, lactic acid greater than 100 mmol L). The non-fermented residue was pyrolyzed at 500 °C to prepare biologically modified biochar (BCB24). In comparison with pristine biochar produced from corn stalks (CB), anaerobic ensiling and anaerobic fermentation significantly increased the surface area, oxygen-containing functional groups, as well as mineral components in BCB24. The maximum sorption capacity of Cd(II) for BCB24 was 2.1 times of CB, suggesting that BCB24 is an effective adsorbent for Cd(II) removal from water. Our results indicated that coupling anaerobic fermentation and pyrolysis technology can significantly improve the efficiency of corn stalks recycling.
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http://dx.doi.org/10.1016/j.wasman.2021.04.027DOI Listing
April 2021

Infectious disease screening in a dedicated primary care clinic for children in foster care.

Child Abuse Negl 2021 Apr 28;117:105074. Epub 2021 Apr 28.

Children's Health System, 1935 Medical District Drive, Dallas, TX 75219, United States.

Background: Children in foster care are considered at high risk for infectious diseases, and guidelines recommend screening for tuberculosis, hepatitis B and C, syphilis, gonorrhea and chlamydia. Little is known about the prevalence of infectious disease in children in foster care.

Objectives: Describe infectious disease screening practices in a primary care clinic dedicated to caring for children in foster care.

Participants And Setting: Patients evaluated at a foster care primary care clinic at a southwestern academic center.

Methods: Retrospective chart review.

Results: From January 1, 2017 through December 31, 2018, 2868 unique patients were evaluated (53 % male, 41 % white, 30 % black, 19 % Hispanic); 1638 (57 %) had any infectious disease laboratory screening done. About 50 % of children had completed screens for tuberculosis, HIV, syphilis and hepatitis C. Tuberculosis screens were positive in 3.6 % of children, 5.5 % of adolescents were positive for chlamydia and <1 % of children were positive for HIV, syphilis or hepatitis C. Increasing age and number of visits were associated with completed tuberculosis, HIV, syphilis and hepatitis C screenings (p < 0.01); female adolescents with completed labs were more likely to be screened for gonorrhea and chlamydia than male adolescents.

Conclusions: Few positive infectious disease screenings were identified in children evaluated in a dedicated foster care primary care clinic despite presence of risk factors. Multiple visits to a primary care foster care clinic may increase the likelihood of completed screenings. Targeted infectious disease screening based on age and local epidemiology may be less traumatizing but still clinically appropriate.
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http://dx.doi.org/10.1016/j.chiabu.2021.105074DOI Listing
April 2021

Characterization of the β-Glucosidase activity in indigenous yeast isolated from wine regions in China.

J Food Sci 2021 Apr 30. Epub 2021 Apr 30.

College of Enology, Northwest A&F University, Yangling, Shaanxi, China.

β-glucosidase is a pivotal enzyme that hydrolyzes bound volatile aromatic compounds. However, the activity of β-glucosidase in winemaking and the mechanism by which it affects the flavor and taste of wines have not been fully investigated. In this study, we profiled the characteristics of β-glucosidase derived from wine-related yeasts isolated from different wine-making regions in China, and analyzed the enzyme activity from different parts of the cells under aerobic and anaerobic conditions. A total of 56 strains of wine-related yeasts producing β-glucosidases were screened using the YNB-C medium (YNB 6.7 g L , cellobiose 5 g L , pH 5.0). We found that strain Clavispora lusitaniae C117 produced the highest enzyme activity (152.39 µmol pNP ml h ). In most strains, β-glucosidase were located in whole cells (periplasmic space) and permeabilized cells (intracellular). The non-Saccharomyces species had the highest enzymatic activity in a strain-dependent manner. Under aerobic conditions, C. lusitaniae C117, Hanseniaspora guilliermondii A27-3-4, Metschnikowia pulcherrima F-1-6, and Pichia anomala C84 had the highest β-glucosidase activity. We further investigated the β-glucosidase activity during the wine fermentation and the effects of sugar, pH, temperature, and ethanol on the enzyme activities of P. anomala C84 and commercial Saccharomyces yeast strains RC212 and VL1. The presence of fructose, glucose, and sucrose strongly inhibited enzyme activity. Similarly, low pH and low temperature inhibited the activity of β-glucosidase, whereas ethanol promoted enzyme activity. Our findings provide a theoretical basis on understanding the different yeast characteristics of β-glucosidase and their potential application for further improving wine aroma complexity.
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http://dx.doi.org/10.1111/1750-3841.15741DOI Listing
April 2021

Large birth size, infancy growth pattern, insulin resistance and β-cell function.

Eur J Endocrinol 2021 Apr 1. Epub 2021 Apr 1.

Z Luo, Department of Obstetrics and Gynecology, Prosserman Centre for Population Health Research, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, University of Toronto, Toronto, Canada.

Objective: Large birth size programs an elevated risk of type 2 diabetes in adulthood, but data are absent concerning glucose metabolic health impact in infancy. We sought to determine whether large birth size is associated with insulin resistance and β-cell function in infancy, and evaluate the determinants.

Design And Participants: In the Canadian 3D birth cohort, we conducted a nested matched (1:2) study of 70 large-for-gestational-age (LGA, birth weight >90th percentile) and 140 optimal-for-gestational-age (OGA, 25th-75th percentiles) control infants. The primary outcomes were homeostasis model assessment of insulin resistance (HOMA-IR) and beta-cell function (HOMA-β) at age 2-years.

Results: HOMA-IR and HOMA-β were similar in LGA and OGA infants. Adjusting for maternal and infant characteristics, decelerated growth in length during early infancy (0-3 months) was associated a 25.8% decrease (95% confidence intervals 6.7-41.0%) in HOMA-β. During mid-infancy (3-12 months), accelerated growth in weight was associated with a 25.5% (0.35-56.9%) increase in HOMA-IR, in length with a 69.3% increase (31.4-118.0%) in HOMA-IR and a 24.5% (0.52-54.3%) increase in HOMA-β. Decelerated growth in length during late infancy (1-2 years) was associated with a 28.4% (9.5-43.4%) decrease in HOMA-IR and a 21.2% (3.9-35.4%) decrease in HOMA-β. Female sex was associated with higher HOMA-β, Caucasian ethnicity with lower HOMA-IR, and maternal smoking with lower HOMA-β.

Conclusions: The study is the first to demonstrate that large birth size is not associated with insulin resistance and β-cell function in infancy, but infancy growth pattern matters. Decelerated infancy growth may be detrimental to beta-cell function.
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http://dx.doi.org/10.1530/EJE-20-1332DOI Listing
April 2021

Invited Response on: Nanofat Injection for the Treatment of Depressed Facial Scars.

Aesthetic Plast Surg 2021 Apr 22. Epub 2021 Apr 22.

Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Badachu Road, Shijingshan District, No. 33, Beijing, 100144, China.

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http://dx.doi.org/10.1007/s00266-021-02283-7DOI Listing
April 2021

Ferroportin 3 is a dual-targeted mitochondrial/chloroplast iron exporter necessary for iron homeostasis in Arabidopsis.

Plant J 2021 Apr 22. Epub 2021 Apr 22.

Department of Biology, Amherst College, Amherst, Massachusetts, 01002, USA.

Mitochondria and chloroplasts are organelles with high iron demand that are particularly susceptible to iron-induced oxidative stress. Despite the necessity of strict iron regulation in these organelles, much remains unknown about mitochondrial and chloroplast iron transport in plants. Here, we propose that Arabidopsis Ferroportin 3 (FPN3) is an iron exporter dual-targeted to mitochondria and chloroplasts. FPN3 is expressed in shoots regardless of iron conditions, but its transcripts accumulate under iron deficiency in roots. fpn3 mutants cannot grow as well as wild type under iron-deficient conditions and their shoot iron levels are lower compared to wild type. Analyses of iron homeostasis gene expression in fpn3 mutants and ICP-MS measurements show that iron levels in the mitochondria and chloroplasts are increased relative to wild type, consistent with the proposed role of FPN3 as a mitochondrial/plastid iron exporter. In iron deficient fpn3 mutants, abnormal mitochondrial ultrastructure was observed, whereas chloroplast ultrastructure was not affected, implying that FPN3 plays a critical role in the mitochondria. Overall, our study suggests that FPN3 is essential for optimal iron homeostasis.
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http://dx.doi.org/10.1111/tpj.15286DOI Listing
April 2021

Effects of plant growth regulator and chelating agent on the phytoextraction of heavy metals by Pfaffia glomerata and on the soil microbial community.

Environ Pollut 2021 Apr 15;283:117159. Epub 2021 Apr 15.

Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China; Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou, 511458, China. Electronic address:

Pfaffia glomerata is a candidate for the remediation of heavy metal-contaminated soil, but phytoremediation efficiency requires enhancement. In this study, we evaluated how application of DA-6, EDTA, or CA affected the growth and heavy metal accumulation of P. glomerata and soil microorganisms. We found that P. glomerata removed more Cd and Zn than Pb or Cu from contaminated soil. When compared to the control, application of DA-6, CA, or CA + DA-6 increased plant biomass and increased stem Cd concentration by 1.28-, 1.20-, and 1.31-fold respectively; increased leaf Cd concentration by 1.25-, 1.28-, and 1.20-fold, respectively; and increased the total quantity of Cd extracted by 1.37-, 1.37-, and 1.38-fold, respectively. When compared to the control, application EDTA or EDTA + DA-6 significantly increased the soil available metal and Na concentrations, which harmed plant growth. Application of EDTA or EDTA + DA-6 also significantly decreased the Cd concentration in roots and stems. 16S rRNA high-throughput sequencing analysis revealed that application of EDTA or CA alone to soil significantly reduced the richness and diversity of soil bacteria, while foliar spraying of DA-6 combined with EDTA or CA slightly alleviated this reduction. EDTA or CA addition significantly changed the proportion of Actinobacteria and Proteobacteria. In addition, EDTA or CA addition caused changes in soil properties (e.g. heavy metal availability, K concentration, Na concentration, soil pH, soil CEC, and soil DOC concentration) that were associated with changes in the bacterial community. EDTA addition mainly affected the soil bacterial community by changing soil DOC concentration, the soil available Pb and Na concentration, and CA addition mainly affected the soil bacterial community by changing the soil available Ca concentration.
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http://dx.doi.org/10.1016/j.envpol.2021.117159DOI Listing
April 2021

Photocatalytic nitrogen fixation of metal-organic frameworks (MOFs) excited by ultraviolet light: insights into the nitrogen fixation mechanism of missing metal cluster or linker defects.

Nanoscale 2021 Apr;13(16):7801-7809

State Key Laboratory of High-efficiency Utilization of Coal and Green Chemical Engineering, School of Chemistry and Chemical Engineering, Ningxia University, Yinchuan, Ningxia 750021, P.R. China.

The vacancies of semiconductors have proven to be effective active sites for photocatalytic nitrogen fixation, but what about the role of defects in MOF materials? Herein, we report the first UiO-66 with photo-excited cluster defects and linker defects for photocatalytic nitrogen fixation. It was determined through the post-synthetic ligand exchange (PSE) process that the linker defects, rather than cluster defects, can greatly improve the performance, which is due to linker defects forming unsaturated metal nodes such as the vacancy in a semiconductor. Specifically, for photo-activated UiO-66, the NH4+ production rate was 196 and 68 μmol g-1 h-1 in air atmosphere under ultraviolet-visible (UV-Vis) and visible light, respectively. This report provides a new effective strategy to design efficient nitrogen fixation photocatalysts.
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http://dx.doi.org/10.1039/d1nr00697eDOI Listing
April 2021

Spherical Formation Tracking Control of Nonlinear Second-Order Agents With Adaptive Neural Flow Estimate.

IEEE Trans Neural Netw Learn Syst 2021 Apr 19;PP. Epub 2021 Apr 19.

This article addresses the spherical formation tracking control problem of nonlinear second-order vehicles moving in flowfields under both undirected networks and directed, strongly connected networks. Different from the previous adaptive estimate of the time-invariant parameters of flowfields, the flowfields under our consideration are spatial and absolutely unknown dynamics. Adaptive neural networks (ANNs) with the novel cooperative adaptive algorithms are proposed to approximate the flowfield acting on the channel of each vehicle's velocity (i.e., the mismatched flowfield) and the flowfield pushing the acceleration (i.e., the matched flowfield), respectively. For the purpose of avoiding the complex derivation derived from backstepping, the novel first-order filters are generated by dynamic surface based on barrier functions and relative positions of neighbors. The proposed control algorithms and adaptive upgrade law are fully distributed without using any global information of the graph. The uniform boundedness is analyzed in the Lyapunov sense. Simulation results are given to verify the theoretical analysis.
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http://dx.doi.org/10.1109/TNNLS.2021.3071317DOI Listing
April 2021

CPA-seq reveals small ncRNAs with methylated nucleosides and diverse termini.

Cell Discov 2021 Apr 19;7(1):25. Epub 2021 Apr 19.

School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China.

High-throughput sequencing reveals the complex landscape of small noncoding RNAs (sRNAs). However, it is limited by requiring 5'-monophosphate and 3'-hydroxyl in RNAs for adapter ligation and hindered by methylated nucleosides that interfere with reverse transcription. Here we develop Cap-Clip acid pyrophosphatase (Cap-Clip), T4 polynucleotide kinase (PNK) and AlkB/AlkB(D135S)-facilitated small ncRNA sequencing (CPA-seq) to detect and quantify sRNAs with terminus multiplicities and nucleoside methylations. CPA-seq identified a large number of previously undetected sRNAs. Comparison of sRNAs with or without AlkB/AlkB(D135S) treatment reveals nucleoside methylations on sRNAs. Using CPA-seq, we profiled the sRNA transcriptomes (sRNomes) of nine mouse tissues and reported the extensive tissue-specific differences of sRNAs. We also observed the transition of sRNomes during hepatic reprogramming. Knockdown of mesenchymal stem cell-enriched U1-5' snsRNA promoted hepatic reprogramming. CPA-seq is a powerful tool with high sensitivity and specificity for profiling sRNAs with methylated nucleosides and diverse termini.
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http://dx.doi.org/10.1038/s41421-021-00265-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8053708PMC
April 2021

An Efficiency of 16.46% and a Lifetime of Over 4000 h for the PM6:Y6 Inverted Organic Solar Cells Enabled by Surface Acid Treatment of the Zinc Oxide Electron Transporting Layer.

ACS Appl Mater Interfaces 2021 Apr 13;13(15):17869-17881. Epub 2021 Apr 13.

School of Nano-Tech and Nano-Bionics, University of Science and Technology of China, Hefei 230027, P. R. China.

For the inverted organic solar cells (OSCs), the interface contacts between the ZnO electron transporting layer and the organic active layer play an important role in the device performance and stability. Since the solution-processed ZnO surface always contains some base or zinc salt contaminants, we explored how the surface pH conditions influence the performance and stability of the nonfullerene acceptor (NFA) cells. A tight relationship between the surface pH condition and the device performance and stability was established. Specifically, device performance and stability were improved by treating the ZnO films with acid solutions but worsened after base treatment. The large number of hydroxyl groups on the surface of the solution-processed ZnO films was proved to be the main reason for the surface pH condition-related performance, which caused oxygen-deficient defects and unfavorable vertical phase separation in the blend films, hindered the photogenerated charge transfer and collection, and consequently resulted in low short-circuit current density and power conversion efficiency (PCE). The surface -OH groups also boosted the photocatalytic activity and led to fast degradation of the nonfullerene acceptor. Removal of the surface -OH groups can alleviate such problems. Different acid solutions, ZrAcac, 2-phenylethylmercaptan (PET), and glutamic acid (GC), were used to treat the ZnO films, and PET treatment was the most effective treatment for performance improvement. An efficiency of 16.46% was achieved for the PM6:Y6 cells and the long-term stability under continuous illumination conditions was significantly improved with a lifetime of over 4000 h (4410 h), showing the excellent long-term stability of this heterojunction solar cell. Our understanding of the surface pH condition-related device performance and stability would guide the development of a feasible method for solving the interface problems in OSCs. We also provide a practical strategy to modify ZnO with acid solutions for high-performance and stable NFA OSCs.
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http://dx.doi.org/10.1021/acsami.1c02613DOI Listing
April 2021

In-situ plasmonic tracking oxygen evolution reveals multistage oxygen diffusion and accumulating inhibition.

Nat Commun 2021 04 12;12(1):2164. Epub 2021 Apr 12.

School of Chemistry and Molecular Engineering, East China Normal University, Shanghai, 200241, China.

Understanding mass transfer processes concomitant with electrochemical conversion for gas evolution reactions at the electrode-electrolyte interface plays a key role in advancing renewable energy storage and conversion. However, due to the complicated diffusion behavior of gas at the dynamic catalytic interfaces, it is still a great challenge to accurately portray mass transfer of gas during electrocatalysis process. Here, we track the diffusion of dissolved oxygen on Cu nanostructured plasmonic interface, which reveals multistage oxygen diffusion behaviors, including premature oxygen accumulation, spontaneous diffusion and accelerated oxygen dissipation. This work uncovers an accumulating inhibition effect on oxygen evolution arising from interfacial dissolved oxygen. With these knowledges, we develop a programmable potential scan strategy to eliminate interfacial gas products, which alleviates the concentration polarization, releases accessible actives sites and promotes electrocatalytic performance. Our findings provide a direct observation of the interfacial mass transfer processes that governs the kinetics of gas-involved multiphases catalysis.
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http://dx.doi.org/10.1038/s41467-021-22434-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8041856PMC
April 2021

Past, present, and perspectives of protein N-terminal methylation.

Curr Opin Chem Biol 2021 Apr 8;63:115-122. Epub 2021 Apr 8.

Department of Medicinal Chemistry and Molecular Pharmacology, Purdue Institute for Drug Discovery, Purdue University Center for Cancer Research, Purdue University, 720 Clinic Drive, West Lafayette, IN, 47907, United States. Electronic address:

The posttranslational methylation of the α-N-terminal amino group of proteins was first documented over 40 years ago, but the functional significance of this modification has been underexplored relative to lysine and arginine methylation. Increasing reports implicates α-N-terminal methylation as a widespread and critical regulator of mitosis, chromatin interactions, DNA repair, and translation fidelity. Here, we summarize advances in the current understanding of protein α-N-terminal methylation biological functions and mechanisms across eukaryotic organisms. Also, we describe the recent literature on substrate recognition and the discovery of potent and selective inhibitors for protein N-terminal methyltransferases. Finally, we summarize the emergent crosstalk between α-N-terminal methylation and other N-terminal modifications.
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http://dx.doi.org/10.1016/j.cbpa.2021.02.017DOI Listing
April 2021

Interaction between β-lactam antibiotic and phosphorus-accumulating organisms.

Environ Sci Pollut Res Int 2021 Apr 1. Epub 2021 Apr 1.

College of Environmental Science and Engineering, Tianjin University, Tianjin, 300350, China.

β-Lactam antibiotics have been widely used in clinic due to strong antibacterial activity with mild adverse side effects and have been detected in the environment. In the enhanced biological phosphorus removal (EBPR) process, phosphorus-accumulating organisms (PAOs) play a major role. In this study, amoxicillin, aztreonam, and cefoperazone are the selected antibiotics that applied in investigating the interaction mechanism of β-lactam antibiotics and PAO. The effects of β-lactam antibiotics on PAOs were analyzed comprehensively from the aspects of antibiotic impacts on phosphorus removal rate, intracellular polymer, their toxicity to PAOs, and PAO impacts on the fate of β-lactam antibiotics. It was found that the phosphorus removal rate of PAO increased by 19.21% and 15.75%, respectively at 10 mg/L amoxicillin and aztreonam, while cefoperazone had certain inhibition effect on phosphorus removal efficiency. Quantitative analysis shows that in the aerobic stage, three kinds of β-lactam antibiotics could promote the synthesis of polyphosphates (poly-P). The degradation rates of three antibiotics were as follows: amoxicillin > aztreonam > cefoperazone. The fate characteristics of antibiotics provide a theoretical basis for environmental risk assessment. The toxic effects of three antibiotics were as follows: cefoperazone > aztreonam > amoxicillin according to the bacteriostatic test. It provided a scientific theoretical basis for systematically evaluating the biological toxicity of antibiotic pollutants.
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http://dx.doi.org/10.1007/s11356-021-13631-0DOI Listing
April 2021

Recharged Catalyst with Memristive Nitrogen Reduction Activity through Learning Networks of Spiking Neurons.

J Am Chem Soc 2021 Apr 31;143(14):5378-5385. Epub 2021 Mar 31.

Department of Chemistry, University of Utah, 315 South 1400 East, Salt Lake City, Utah 84112, United States.

Electrocatalysis from N to NH has been increasingly studied because it provides an environmentally friendly avenue to take the place of the current Haber-Bosch method. Unfortunately, the conversion of N to NH is far below the necessary level for implementation at a large scale. Inspired by signal memory in a spiking neural network, we developed rechargeable catalyst technology to activate and remember the optimal catalytic activity using manageable electrical stimulation. Herein, we designed double-faced FeReS Janus layers that mimic a multiple-neuron network consisting of resistive switching synapses, enabling a series of intriguing multiphase transitions to activate undiscovered catalytic activity; the activation energy barrier is clearly reduced via an active site conversion between two nonequivalent surfaces. Electrical field-stimulated FeReS demonstrates a Faradaic efficiency of 43% and the highest rate of 203 μg h mg toward NH synthesis. Moreover, this rechargeable catalyst displays unprecedented catalytic performance that persists for up to 216 h and can be repeatedly activated through a simple charging operation.
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http://dx.doi.org/10.1021/jacs.0c12458DOI Listing
April 2021

Preliminary study on clinical characteristics of Chinese patients with positional obstructive sleep apnea.

Sleep Breath 2021 Mar 30. Epub 2021 Mar 30.

Department of Respiratory and Critical Care Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, No.1 Shuaifuyuan Street, Dongcheng District, Beijing, 100730, China.

Purpose: The objective of this study was to evaluate the prevalence, the clinical characteristics, and the possible predictors of Chinese patients with positional obstructive sleep apnea (POSA) according to the Amsterdam Positional Obstructive Sleep Apnea Classification (APOC).

Methods: A retrospective study in the sleep unit of Peking Union Medical College Hospital was conducted to analyze the clinical and polysomnography data of Chinese patients with obstructive sleep apnea (OSA).

Results: Of 372 patients with OSA, 54% met the APOC criteria for POSA. The prevalence of POSA was significantly higher in women with OSA than in men. Chinese patients with POSA had a lower apnea-hypopnea index, oxygen desaturation index, and the percentage of time spent at oxygen saturation below 90% in total sleep time; and a higher mean oxygen saturation (SaO) and minimum SaO during sleep, which were remarkable in the APOC I group. By multivariate logistic regression analyses, the higher mean SaO (≥95%) during sleep and mild and moderate OSA were positive predictors of POSA. Mild and moderate OSA was the independent predictor of POSA in women. Higher mean SaO (≥95%) during sleep was the independent predictor of POSA in men.

Conclusion: According to the APOC, the prevalence of POSA is high in Chinese patients with OSA, especially in women. Chinese patients with POSA had less severe OSA and a lower degree of nocturnal hypoxia, which was remarkable in the APOC I group.
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http://dx.doi.org/10.1007/s11325-021-02346-8DOI Listing
March 2021

CuBiS/MXenes with Excellent Solar-Thermal Conversion for Continuous and Efficient Seawater Desalination.

ACS Appl Mater Interfaces 2021 Apr 30;13(14):16246-16258. Epub 2021 Mar 30.

Key Laboratory of Polar Materials and Devices (MOE), Department of Electronics, East China Normal University, Shanghai 200241, China.

Two-dimensional materials with unique physical and chemical properties have recently attracted widespread attention in the field of solar thermal conversion. However, affected by the Fresnel effect, traditional two-dimensional materials such as MXenes, graphene, transition metal disulfide often have relatively significant light reflection losses at the solid-liquid or gas interface. So how to improve the light absorption of the two-dimensional material performance has become a new challenge in photothermal conversion. Here, we use an improved thermal-injection method to uniformly grow Tricopper(I) Bismuth Sulfide (CuBiS, CBS) on the surface of TiC nanosheets in a nonaqueous polar solvent environment. A three-dimensional nanoflower-nanosheet structure CBS-TiC for photothermal conversion has been constructed successfully. Owing to the excellent photothermal performance of CuBiS in the near-infrared region, the good thermal conductivity of TiC, and the unique porous structure of the composite material, the composite achieves broadband absorption of light (more than 90% in the visible light region, more than 80% in the near-infrared region), which optical model and finite element simulation have theoretically verified. The composite material has obtained higher solar-to-heat conversion performance than similar material systems, and the steady-state temperature can reach 62.3 °C under 1 sun incident light intensity. CBS-TiC is expected to become a light-absorbing layer material for solar vapor generation devices due to its excellent light-to-heat conversion performance and good material flexibility. It still guarantees a reasonably high steam generation rate (1.32 kg·m·h) even with a thinner material thickness (0.48 mg·cm) and a comprehensive conversion efficiency higher than 90%. Besides, CBS-TiC also exhibits the characteristics of resisting surface salt accumulation, which is conducive to maintaining the long-lasting photothermal seawater evaporation process. The material's electronic structure and the charge transfer process of the heterojunction interface have been studied with the first-principles calculation. The high light absorption performance and good thermal conductivity of the composite material are theoretically explained and supported.
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http://dx.doi.org/10.1021/acsami.0c22761DOI Listing
April 2021

Molecular mechanisms, immune cell infiltration, and potential drugs for prostate cancer.

Cancer Biomark 2021 Mar 23. Epub 2021 Mar 23.

Institute of Urology and Nephrology, the First Affiliated Hospital of Guangxi Medical University, Guangxi Medical University, Guangxi, China.

Background: The molecular mechanisms involved in the prostate cancer and their relationship with immune cell infiltration are not fully understood. The prostate cancer patients undergoing standard androgen deprivation therapy eventually develop castration resistant prostate cancer (CRPC) for which there is no effective treatment currently available, and the hub genes involved in this process remain unclear.

Objective: To study prostate cancer systematically and comprehensively.

Methods: Differentially expressed genes (DEGs) of prostate cancer were screened in The Cancer Genome Atlas (TCGA) database. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed. Connectivity Map (Cmap) software was applied to discover potential treatment drugs. A protein-protein interaction (PPI) analysis was performed to obtained the hub genes, and the relationship between hub genes and immune cell infiltration was investigated. Next, RNAseq data of hormone-sensitive prostate cancer samples and CRPC samples obtained from TCGA database was further analyzed to identify DEGs. Finally, a PPI analysis was performed to obtain the hub genes.

Results: A total of 319 DEGs were identified between prostate cancer samples and normal adjacent samples from TCGA database using comparative analysis. The KEGG pathway analysis showed significant correlations with drug metabolism, metabolism of xenobiotics by cytochrome P450, and chemical carcinogenesis. AMACR, FOLH1 and NPY, three hub genes, were found to be upregulated. FOLH1 was positively correlated with CD8+ T cell infiltration. FOLH1, AMACR, and NPY were negatively correlated with CD4+ T cell infiltration. A total of 426 DEGs were identified from RNAseq data of hormone-sensitive prostate cancer samples and CRPC samples using further comparative analysis. KEGG pathway enrichment analysis showed significant correlations with arachidonic acid metabolism, PPAR signaling pathway, AMPK signaling pathway, and metabolic.
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http://dx.doi.org/10.3233/CBM-200939DOI Listing
March 2021

An Environmentally Benign Cascade Reaction of 1,1-Enediamines (EDAMs) for Site-Selective Synthesis of Highly Functionalized 2,10-Dihydro-1-imidazo[1',2':1,6]pyrido[2,3-]indoles and Pyrroles.

J Org Chem 2021 Apr 27;86(8):5744-5756. Epub 2021 Mar 27.

Key Laboratory of Medicinal Chemistry for Natural Resource (Yunnan University), Ministry of Education, School of Chemical Science and Technology, Yunnan University, Kunming, 650091, P. R. China.

A novel protocol for the synthesis of pyrido[2,3-]indoles (α-carbolines, ) from ()-3-(2-oxo-2-phenylethylidene) indolin-2-one derivatives and 1,1-enediamine (EDAM) via an unexpected cascade reaction in ethanol was developed. Pyrido[2,3-]indole derivatives were obtained by the same reaction, albeit by stirring the mixture for a longer period of time (about 48 h). As a result, two kinds of functionalized α-carbolines and were synthesized by the facile reaction of the ()-3-(2-oxo-2-phenylethylidene)indolin-2-one derivatives and 2-(nitromethylene)imidazolidine under basic conditions (CsCO) in ethanol. In addition, a diverse array of EDAM substrates (-) were tested in this reaction to afford the expected target compounds . This protocol is suitable for the combinatorial and parallel syntheses of natural-like products, including highly functionalized α-carbolines and pyrroles, especially 2-oxoindolin-3-yl pyrroles. This approach features several advantages, such as being a simple and practical operation (requiring only filtration and washing without column chromatography), furnishing excellent yields (72-98%), and producing diverse libraries of target compounds with potential biological activities.
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http://dx.doi.org/10.1021/acs.joc.1c00211DOI Listing
April 2021

Chloroplast genomes of two Mediterranean Bupleurum species and the phylogenetic relationship inferred from combined analysis with East Asian species.

Planta 2021 Mar 25;253(4):81. Epub 2021 Mar 25.

School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, 510515, China.

Main Conclusion: The chloroplast genomes of Mediterranean Bupleurum species are reported for the first time. Phylogenetic analysis supports the species as a basal clade of Bupleurum with divergence time at 35.40 Ma. Bupleurum is one of the most species-rich genus with high medicinal value in Apiaceae. Although infrageneric classifications of Bupleurum have been the subject of numerous studies, it still remains controversial. Chloroplast genome information will prove essential in advancing our understanding on phylogenetic study. Here we report cp genomes of two woody Bupleurum species (Bupleurum gibraltaricum and B. fruticosum) endemic to Mediterranean. The complete cp genomes of the two species were 157,303 and 157,391 bp in size, respectively. They encoded 114 unique genes including 30 tRNA genes, 4 rRNA genes and 80 protein coding genes. Genome structure, distributions of SDRs and SSRs, gene content exhibited similarities among Bupleurum species. High variable hotspots were detected in eight intergenic spacers and four genes. Most of genes were under purifying selection with two exceptions: atpF and clpP. The phylogenetic analysis based on 80 coding genes revealed that the genus was divided into 2 distinct clades corresponding to the 2 subgenera (subg. Penninervia, subg. Bupleurum) with divergence time at the end of collision of India with Eurasia. Most species diversified mainly during the later period of uplift of Qinghai-Tibetan Plateau. The cp genomes of the two Bupleurum species can be significant complementary to insights into the cp genome characteristics of this genus. The comparative chloroplast genomes and phylogenetic analysis advances our understanding of the evolution of cp genomes and phylogeny in Bupleurum.
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http://dx.doi.org/10.1007/s00425-021-03602-7DOI Listing
March 2021

HDAC11 inhibition disrupts porcine oocyte meiosis via regulating α-tubulin acetylation and histone modifications.

Aging (Albany NY) 2021 03 19;13(6):8849-8864. Epub 2021 Mar 19.

Key Laboratory of Organ Regeneration and Transplantation of Ministry of Education, First Hospital, Jilin University, Changchun 130021, Jilin, China.

HDAC11, the sole member of HDAC class IV family, plays vital roles in activating mitosis and apoptosis of tumor cells, but its functions in meiosis are rarely investigated. In the present study, the effect of HDAC11 on meiosis during porcine oocytes maturation was fully studied. The results showed that HDAC11 inhibition by its specific inhibitor JB-3-22 dramatically decreased the porcine oocyte maturation rate by disturbing spindle organization and chromosomes alignment without affecting the cytoplasmic maturation. Further study indicated that HDAC11 inhibition significantly elevated the acetylation levels of α-tubulin and H4K16, which are crucial for spindle organization and chromosomes alignment. Moreover, immunofluorescence staining results showed that HDAC11 inhibition also disturbed other meiosis-related histone modifications, such as increased H3S10pho, H4K5ac and H4K12ac levels and reduced H3T3pho level. Furthermore, RNA-seq analysis results indicated that HDAC11 inhibition disturbed porcine oocytes transcriptome (157 up-regulation, 106 down-regulation). In addition, HDAC11 inhibition compromised oocytes quality and subsequent development after parthenogenetic activation, which may be caused by the aberrant nuclear maturation and transcriptome expression profile during oocytes maturation. Therefore, our results elucidate the function of HDAC11 in porcine oocytes maturation and embryos development through regulating α-tubulin acetylation, meiosis-related histone modifications and transcriptome.
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http://dx.doi.org/10.18632/aging.202697DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034937PMC
March 2021

Cord blood IGF-I, proinsulin, leptin, HMW adiponectin and ghrelin in short or skinny small-for-gestational-age infants.

J Clin Endocrinol Metab 2021 Mar 19. Epub 2021 Mar 19.

Ministry of Education-Shanghai Key Laboratory of Children's Environmental Health, Department of Behavioral Pediatrics and Child Primary Care, Xinhua Hospital, Shanghai Jiao-Tong University School of Medicine, Shanghai, China.

Context: Small-for-gestational-age (SGA) is an indicator of poor fetal growth "programming" an elevated risk of type 2 diabetes in adulthood. Little is known about early life endocrine characteristics in SGA subtypes. Stunting (short) and wasting (skinny) are considered distinct SGA phenotypes in neonatal prognosis.

Objectives: To assess whether SGA infants with stunting or wasting have similar alterations in neonatal endocrine metabolic health biomarkers.

Design: A nested case-control study.

Setting: The 3D (Design, Develop and Discover) birth cohort in Canada.

Participants: 146 SGA (birth weight <10 th percentile) and 155 optimal for gestational age (OGA, 25 th-75 th percentiles) infants. Stunting was defined as birth length <10 th percentile, and wasting as body mass index <10 th percentile for sex and gestational age, respectively.

Main Outcomes: Cord plasma concentrations of insulin-like growth factor I (IGF-I), proinsulin, leptin, high-molecular-weight (HMW) adiponectin and ghrelin.

Results: Comparing to OGA infants adjusted for maternal and neonatal characteristics, SGA infants with either stunting only or wasting only had lower cord plasma IGF-I and leptin concentrations. HMW adiponectin concentrations were lower in SGA infants with wasting only (P=0.004), but similar in SGA infants with stunting only (P=0.816). Only SGA infants with both stunting and wasting had substantially lower proinsulin (P<0.001) and higher ghrelin concentrations (P<0.001) than OGA infants.

Conclusions: The study is the first to demonstrate that SGA infants with wasting only are characterized by low HMW adiponectin concentrations, while those with stunting only are not. SGA with both stunting and wasting are characterized by low proinsulin and high ghrelin concentrations.
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http://dx.doi.org/10.1210/clinem/dgab178DOI Listing
March 2021

Structure-based Discovery of Cell-Potent Peptidomimetic Inhibitors for Protein N-Terminal Methyltransferase 1.

ACS Med Chem Lett 2021 Mar 1;12(3):485-493. Epub 2021 Mar 1.

Department of Medicinal Chemistry and Molecular Pharmacology, Purdue Institute for Drug Discovery, Purdue University Center for Cancer Research, Purdue University, West Lafayette, Indiana 47907, United States.

Protein N-terminal methyltransferases (NTMTs) catalyze the methylation of the α-N-terminal amines of proteins starting with an X-P-K/R motif. NTMT1 has been implicated in various cancers and in aging, implying its role as a potential therapeutic target. Through structural modifications of a lead NTMT1 inhibitor, , we designed and synthesized a diverse set of inhibitors to probe the NTMT1 active site. The incorporation of a naphthyl group at the N-terminal region and an -aminobenzoic amide at the C-terminal region of generates the top cell-potent inhibitor , demonstrating increased activity on both purified NTMT1 (IC of 0.34 ± 0.02 μM) and the cellular α-N-terminal methylation level of regulator of chromosome condensation 1 (RCC1, IC value of 30 μM) in human colorectal cancer HT29 cells. Furthermore, exhibits over 300-fold selectivity to several methyltransferases. This study points out the direction for the development of more cell-potent inhibitors for NTMT1.
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http://dx.doi.org/10.1021/acsmedchemlett.1c00012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7958150PMC
March 2021

Semiconducting and Metallic [5,5] Fullertube Nanowires: Characterization of Pristine D(1)-C and D(1)-C.

J Am Chem Soc 2021 Mar 18;143(12):4593-4599. Epub 2021 Mar 18.

Department of Chemistry, Virginia Tech, Blacksburg, Virginia 24061, United States.

Although fullerenes were discovered nearly 35 years ago, scientists still struggle to isolate "single molecule" tubular fullerenes larger than C. In similar fashion, there is a paucity of reports for pristine single-walled carbon nanotubes (SWNTs). In spite of Herculean efforts, the isolation and properties of pristine members of these carbonaceous classes remain largely unfulfilled. For example, the low abundance of spherical and tubular higher fullerenes in electric-arc extracts (<0.01-0.5%) and multiplicity of structural isomers remain a major challenge. Recently, a new isolation protocol for highly tubular , also called f ullertubes, was reported. Herein, we describe spectroscopic characterization including C NMR, XPS, and Raman results for purified [5,5] fullertube family members, D-C and D-C. In addition, DFT computational HOMO-LUMO gaps, polarizability indices, and electron density maps were also obtained. The Raman and C NMR results are consistent with semiconducting and metallic properties for D-C and D-C, respectively. Our report suggests that short [5,5] fullertubes with aspect ratios of only ∼1.5-2 are metallic and could exhibit unique electronic properties.
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http://dx.doi.org/10.1021/jacs.0c11357DOI Listing
March 2021

CircRNA-016901 silencing attenuates irradiation-induced injury in bone mesenchymal stem cells via regulating the miR-1249-5p/HIPK2 axis.

Exp Ther Med 2021 Apr 11;21(4):355. Epub 2021 Feb 11.

Department of Laboratory Medicine, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan 410013, P.R. China.

Currently, bone marrow transplantation remains the basic treatment for various hematological tumors and irradiation is one of the most important pretreatment methods. However, irradiation pretreatment may result in damage to bone mesenchymal stem cells (BMSCs). The present study aimed to investigate the effect of circular RNA-016901 (circ-016901) on the injury of irradiation-induced BMSCs and the underlying mechanism. The expression levels of circ-016901, microRNA-1249-5p (miR-1249-5p) and homeodomain interacting protein kinase 2 (HIPK2) in irradiation-induced mouse BMSCs at various irradiation doses were detected via reverse transcription-quantitative PCR (RT-qPCR). The effect of circ-016901 on cell proliferation was examined using Cell Counting Kit-8 assays following silencing or overexpression of circ-016901. Cell apoptosis was detected by flow cytometry and caspase-3/7 activity. The expression of autophagy-related markers, including Beclin-1 and LC3-II/I, was detected at the mRNA and protein levels by RT-qPCR and western blotting, respectively. Irradiation treatment upregulated the expression of circ-016901 and HIPK2 and downregulated miR-1249-5p expression. The expression levels of LC3-II/I and Beclin-1 in BMSCs were downregulated in a dose-dependent manner. Silencing of circ-016901 promoted proliferation of irradiation-induced BMSCs and attenuated irradiation-induced apoptosis. Moreover, silencing of circ-016901 elevated the expressions of LC3-II/I and Beclin-1 in irradiation-induced BMSCs. Similar results were obtained with miR-1249-5p overexpression and HIPK2 silencing. These results demonstrated that circ-016901 silencing attenuated injury in irradiation-induced mouse BMSCs by regulating the miR-1249-5p/HIPK2 axis, providing a novel target for future research on the mechanism of radiation resistance in BMSCs.
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http://dx.doi.org/10.3892/etm.2021.9786DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7903417PMC
April 2021

Predictive value of blood concentration of biologics on endoscopic inactivity in inflammatory bowel disease: A systematic review.

World J Gastroenterol 2021 Mar;27(9):886-907

Department of Gastroenterology, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310006, Zhejiang Province, China.

Background: Although blood concentration of biologics is an important composition of disease management in inflammatory bowel disease (IBD) patients, complexity and uncertainty of biological management encourage many disputes in predicting the outcome of IBD patients through blood concentration of biologics.

Aim: To verify the predictive value of blood concentration of biologics on endoscopic inactivity in IBD patients under different situations.

Methods: We searched PubMed/MEDLINE, Embase, and Web of Science up to May 2020 and identified IBD patients as the research cohort as well as the correlations between blood concentration of biologics and endoscopic inactivity in IBD patients as the research direction.

Results: A total of 23 articles with 30 clinical studies and 1939 IBD patients were included. The predictive cut-off value of blood concentration of infliximab on mucosal healing should be 2.7-10.6 μg/mL in IBD. Blood concentration of infliximab reaching 5.0-12.7 μg/mL or more increased the probability of fistula healing/closure in perianal fistulizing Crohn's disease. Blood concentration of adalimumab reaching 7.2-16.2 μg/mL or more could predict mucosal healing in IBD. The predictive cut-off value of blood concentration of adalimumab on fistula healing/closure should be 5.9-9.8 μg/mL in perianal fistulizing Crohn's disease. Blood concentration of vedolizumab surpassing 25.0 μg/mL indicated mucosal healing in ulcerative colitis patients under maintenance therapy and the predictive cut-off value of blood concentration on mucosal healing or endoscopic remission under induction therapy in IBD could be 8.0-28.9 μg/mL.

Conclusion: Blood concentration of biologics should not be utilized to predict endoscopic inactivity of IBD independently due to discrepancies in clinical studies, whereas conducting therapeutic drug monitoring intensively contributes to precise therapy.
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http://dx.doi.org/10.3748/wjg.v27.i9.886DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7941861PMC
March 2021