Publications by authors named "Rong Gu"

61 Publications

Colchicine alleviates inflammation and improves diastolic dysfunction in heart failure rats with preserved ejection fraction.

Eur J Pharmacol 2022 Aug 30;929:175126. Epub 2022 Jun 30.

Department of Cardiology, Affiliated Drum Tower Hospital, Medical School of Nanjing University, Nanjing, 210008, PR China. Electronic address:

Purpose: Several studies have reported that colchicine attenuates cardiac inflammation and improves cardiac function in myocardial infarction and atrial fibrillation. However, no study has investigated its effect on heart failure with preserved ejection fraction (HFpEF). Hence, this study aimed to assess its efficacy in a high salt diet (HSD)-induced HFpEF rat model.

Methods: A rat hypertension-induced HFpEF model was created by treating Dahl/SS salt-sensitive rats with an HSD for 6 weeks. Colchicine was given via gavage daily as treatment. Cardiac function and inflammation were assessed using echocardiography, histology, and ELISA. Furthermore, the expression levels of NLRP3 and NF-κB signaling pathways were examined.

Results: Treatment with colchicine increased survival and attenuated cardiac dysfunction, as indicated by decreased echocardiographic E/A ratio and longer exercise endurance along with reduced ventricular fibrosis and remodeling in HSD-induced Dahl rats. The treatment also reduced cardiac oxidative stress and inflammatory cell infiltration, as inferred from lower mRNA expressions of TNFα and CCL2 as well as protein expressions of NLRP3 and NF-κB pathways.

Conclusion: The findings signify that colchicine plays a crucial role in alleviating systemic inflammation and NLRP3 inflammation activation as well as in attenuating cardiac dysfunction and fibrosis in HSD-induced HFpEF model. Colchicine, therefore, holds therapeutic potential for further clinical applications.
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http://dx.doi.org/10.1016/j.ejphar.2022.175126DOI Listing
August 2022

Colchicine Ameliorates Dilated Cardiomyopathy Via SIRT2-Mediated Suppression of NLRP3 Inflammasome Activation.

J Am Heart Assoc 2022 Jul 29;11(13):e025266. Epub 2022 Jun 29.

State Key Laboratory of Pharmaceutical Biotechnology, Department of Cardiology, Nanjing Drum Tower Hospital Medical School of Nanjing University Nanjing China.

Background Dilated cardiomyopathy remains a leading cause of heart failure worldwide. Immune inflammation response is recognized as a significant player in the progression of heart failure; however, immunomodulatory strategies remain a long-term challenge. Colchicine, a potent anti-inflammatory drug, has many benefits in ischemic cardiovascular events, but its role in nonischemic heart failure remains unclear. Methods and Results Doxorubicin administration was used to establish a murine dilated cardiomyopathy model, and colchicine or saline was orally given. At the end point, cardiac function and fibrosis were measured to investigate the effects of colchicine. Inflammatory cytokine levels, neutrophil recruitment, and NLRP3 (NOD-like receptor protein 3) inflammasome activation were detected to evaluate the inflammatory response. Furthermore, to examine the downstream target of colchicine, SIRT2 (Sirtuin 2) was pharmacologically inhibited in vitro; thus, changes in the NLRP3 inflammasome were detected by immunoblotting. These results showed that murine cardiac function was significantly improved and fibrosis was significantly alleviated after colchicine treatment. Moreover, the infiltration of neutrophils and the levels of inflammatory cytokines in the failing myocardium were both decreased by colchicine treatment. Mechanistically, colchicine upregulated the expression of SIRT2, leading to the inactivation of the NLRP3 inflammasome in an NLRP3 deacetylated manner. Conversely, the inhibition of SIRT2 attenuated the suppressive effect of colchicine on NLRP3 inflammasome activation. Conclusions This study indicated that colchicine could be a promising therapeutic candidate for dilated cardiomyopathy and other nonischemic heart failure associated with the inflammatory response.
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http://dx.doi.org/10.1161/JAHA.122.025266DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9333380PMC
July 2022

Prognostic value of triglyceride glucose (TyG) index in patients with acute decompensated heart failure.

Cardiovasc Diabetol 2022 05 31;21(1):88. Epub 2022 May 31.

Department of Cardiology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, 210008, Jiangsu, China.

Background: The triglyceride glucose (TyG) index has been proposed as a reliable marker of insulin resistance (IR) and an independent predictor of cardiovascular disease risk. However, its prognostic value in patients with acute decompensated heart failure (ADHF) remains unclear.

Methods: A total of 932 hospitalized patients with ADHF from January 1st, 2018 to February 1st, 2021 were included in this retrospective study. The TyG index was calculated as ln [fasting triglyceride level (mg/dL) × fasting plasma glucose level (mg/dL)/2]. Patients were divided into tertiles according to TyG index values. The primary endpoints were all-cause death, cardiovascular (CV) death and major adverse cardiac and cerebral events (MACCEs) during follow-up. We used multivariate adjusted Cox proportional hazard models and restricted cubic spline analysis to investigate the associations of the TyG index with primary endpoints.

Results: During a median follow-up time of 478 days, all-cause death, CV death and MACCEs occurred in 140 (15.0%), 103 (11.1%) and 443 (47.9%) cases, respectively. In multivariate Cox proportional hazard models, the risk of incident primary endpoints was associated with the highest TyG tertile. After adjustment for confounding factors, hazard ratios (HRs) for the highest tertile (TyG index ≥ 9.32) versus the lowest tertile (TyG index < 8.83) were 2.09 (95% confidence interval [CI], 1.23-3.55; p = 0.006) for all-cause death, 2.31 (95% CI, 1.26-4.24; p = 0.007) for CV death and 1.83 (95% CI, 1.18-3.01; p = 0.006) for MACCEs. Restricted cubic spline analysis also showed that the cumulative risk of primary endpoints increased as TyG index increased. When the TyG index was used as a continuous variable, the hazard ratios of the three primary endpoints rapidly increased within the higher range of the TyG index (all cause death, TyG > 9.08; CV death, TyG > 9.46; MACCEs, TyG > 9.87).

Conclusions: The elevated TyG index was independently associated with poor prognosis, and thus would be useful in the risk stratification in patients with ADHF.
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http://dx.doi.org/10.1186/s12933-022-01507-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9158138PMC
May 2022

Renal Denervation Attenuates Adverse Remodeling and Intramyocardial Inflammation in Acute Myocardial Infarction With Ischemia-Reperfusion Injury.

Front Cardiovasc Med 2022 28;9:832014. Epub 2022 Apr 28.

Department of Cardiology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, China.

Background: Inhibition of sympathetic activity and renin-angiotensin system with renal denervation (RDN) was proved to be effective in managing refractory hypertension, and improving left ventricular (LV) performance in chronic heart failure. The inhibition of sustained sympathetic activation prevents or delays the development of cardiac fibrosis and dysfunction that occurs after myocardial infarction and ischemia-reperfusion (I/R) injury. The translational efficiency of RDN remains to be defined in preclinical animal studies.

Objectives: This study investigated the therapeutic role of RDN in adverse remodeling and intramyocardial inflammation in myocardial ischemia-reperfusion (MI/R) injury.

Methods: Herein, 15 minipigs were subjected to 90-min percutaneous occlusion of the left anterior descending artery followed by reperfusion. Eight animals received simultaneous RDN using catheter-based radiofrequency ablation (MI/R-RDN). Cardiac function and infarct volume were measured , followed by histological and biochemical analyses.

Results: The infarct volume in I/R-RDN pigs reduced at 30 days postreperfusion, compared to I/R-Sham animals. The levels of catecholamine and cytokines in the serum, kidney cortex, the border, and infarcted regions of the heart were significantly reduced in I/R-RDN group. Moreover, the gene expression of collagen and the protein expression of adrenergic receptor beta 1 in heart were also decreased in I/R-RDN mice. Additionally, RDN therapy alleviated myocardial oxidative stress.

Conclusion: RDN is an effective therapeutic strategy for counteracting postreperfusion myocardial injury and dysfunction, and the application of RDN holds promising prospects in clinical practice.
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http://dx.doi.org/10.3389/fcvm.2022.832014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9095912PMC
April 2022

Optimizing the Bacteriostatic and Cytocompatibility Properties of Poly(hexamethylene guanidine) Hydrochloride (PHMG) via the Guanidine/Alkane Ratio.

Biomacromolecules 2022 05 24;23(5):2170-2183. Epub 2022 Apr 24.

State and Local Joint Engineering Laboratory for Novel Functional Polymeric Materials, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Ren'ai Road, Suzhou 215123, P. R. China.

The emergence of "superbugs" is not only problematic and potentially lethal for infected subjects but also poses serious challenges for the healthcare system. Although existing antibacterial agents have been effective in some cases, the side effects and biocompatibility generally present difficulties. The development of new antibacterial agents is therefore urgently required. In this work, we have adapted a strategy for the improvement of poly(hexamethylene guanidine) hydrochloride (PHMG), a common antibacterial agent. This involves copolymerization of separate monomer units in varying ratios to find the optimum ratio of the hydrocarbon to guanidine units for antibacterial activity. A series of these copolymers, designated as PGB, was synthesized. By varying the guanidine/hydrophobic ratio and the copolymer molecular weight, a structure-optimized PGB was identified that showed broad-spectrum antibacterial activity and excellent biocompatibility in solution. In an antibacterial assay, the copolymer with the optimum composition (hydrophobic unit content 25%) inhibited >99% and was compatible with mammalian cells. A polyurethane emulsion containing this PGB component formed transparent, flexible films (PGB-PU films) on a wide range of substrate surfaces, including soft polymers and metals. The PGB-PU films showed excellent bacteriostatic efficiency against nosocomial drug-resistant bacteria, such as and methicillin-resistant (MRSA). It is concluded that our PGB polymers can be used as bacteriostatic agents generally and in particular for the design of antibacterial surfaces in medical devices.
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http://dx.doi.org/10.1021/acs.biomac.2c00233DOI Listing
May 2022

Robust, anti-biofouling 2D nanogel films from poly(-vinyl caprolactam--vinylimidazole) polymers.

J Mater Chem B 2022 05 18;10(19):3723-3733. Epub 2022 May 18.

State and Local Joint Engineering Laboratory for Novel Functional Polymeric Materials, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, P. R. China.

In analogy with adsorbed protein films, we have fabricated a family of 2D nanofilms composed of poly(-vinyl caprolactam--vinylimidazole) (PNVCL) nanogels. NVCL was copolymerized with 1-vinylimidazole (VIM), and then cross-linked with α,ω-dibromoalkanes with 2 to 8 carbons quaternization to form the nanogels. The swelling ratio of the gels was precisely controlled by regulating the inter-chain spacing of the polymers at the level of the carbon atom chain length of the cross-linker. The short-chain alkanes used are relatively rigid and their dimensions provide an accurate estimate of the chain spacing in the nanogels. It was shown that small differences in the carbon atom number of the cross-linking agent led to significant differences in the mechanical properties of the nanogels, in particular in the softness, deformability, and contact area (in film form), all of which increased with increasing carbon number. Films of the softer gels not only showed good adhesion to a number of substrates, but were also mechanically robust. In addition, the films showed excellent light transmission and nontoxicity to L929 cells. Nanogels of intermediate softness were shown to inhibit the adhesion of bacteria and human umbilical vein smooth muscle cells (HUVSMCs), and to be resistant to the adsorption of the plasma protein fibrinogen, indicating strong anti-biofouling properties. Gels that were either too stiff or too soft showed somewhat weaker anti-fouling activity in terms both of HUVSMCs adhesion and protein adsorption.
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http://dx.doi.org/10.1039/d1tb02726cDOI Listing
May 2022

Major vault protein attenuates cardiomyocyte injury in doxorubicin-induced cardiomyopathy through activating AKT.

BMC Cardiovasc Disord 2022 03 4;22(1):77. Epub 2022 Mar 4.

Department of Cardiology, Nanjing Drum Tower Hospital, Affiliated Hospital of Nanjing University Medical School, Nanjing, 210008, China.

Background: Doxorubicin (DOX) has limited chemotherapy application for malignancies due to cardiotoxicity. The pathogenesis of DOX-induced cardiomyopathy (DiCM) is yet to be elucidated. Increasing studies proved that activation of AKT prevented cardiomyocyte apoptosis and cardiac dysfunction in response to DOX insult. Our previous studies indicated that major vault protein (MVP) deficiency was accompanied by suppressed phosphorylation of AKT in metabolic diseases. This study aimed to investigate the role and underlying mechanism of MVP on cardiomyocyte apoptosis in DiCM.

Methods: Mice were intraperitoneally injected with DOX 5 mg/kg, once a week for 5 weeks, the total cumulative dose was 25 mg/kg. Cardiomyocyte-specific MVP overexpression was achieved using an adeno-associated virus system under the cTnT promoter after the fourth DOX injection. Cardiac function was examined by echocardiography followed by euthanasia. Tissue and serum were collected for morphology analysis and biochemical examination.

Results: Herein, we found that MVP expression was upregulated in DOX-treated murine hearts. Cardiac-specific MVP overexpression alleviated DOX-induced cardiac dysfunction, oxidative stress and fibrosis. Mechanistically, MVP overexpression activated AKT signaling and decreased cardiomyocyte apoptosis in DiCM.

Conclusions: Based on these findings, we supposed that MVP was a potential therapeutic agent against DiCM.
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http://dx.doi.org/10.1186/s12872-022-02517-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8896232PMC
March 2022

One-step surface modification strategy with composition-tunable microgels: From bactericidal surface to cell-friendly surface.

Colloids Surf B Biointerfaces 2022 Apr 29;212:112372. Epub 2022 Jan 29.

State and Local Joint Engineering Laboratory for Novel Functional Polymeric Materials, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, PR China. Electronic address:

As modifiers for biomaterial surfaces, soft colloidal particles not only have good film-forming properties, but can also contribute to the function of the biomaterial via their chemical and biological properties. This general approach has proven effective for surface modification, but little is known about methods to control the properties of the colloidal particles to regulate film formation and biological function. In this work, we prepared poly (N-isopropylacrylamide) microgels (ZQP) containing both a zwitterionic component (Z) to provide anti-fouling functionality, and a quaternary ammonium salt (Q) to give bactericidal functionality. Fine-tuning of the Z and Q contents allowed the preparation of microgels over a range of particle size, size distribution, charge, and film-forming capability. The films showed anti-adhesion and contact-killing properties versus Escherichia coli (E. Coli), depending on the chemical composition. They also showed excellent cytocompatibility relative to L929 cells. A variety of microgel-coated substrates (silicon wafer, PDMS, PU, PVC) showed long-term anti-bacterial activity and resistance to chemical and mechanical treatments. It is concluded that this approach allows the preparation of effective bactericidal, cytocompatible surfaces. The properties can be fine-tuned by regulation of the microgel composition, and the method is applicable universally, i.e., independent of substrate.
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http://dx.doi.org/10.1016/j.colsurfb.2022.112372DOI Listing
April 2022

High-Z-Sensitized Radiotherapy Synergizes with the Intervention of the Pentose Phosphate Pathway for In Situ Tumor Vaccination.

Adv Mater 2022 Apr 20;34(13):e2109726. Epub 2022 Feb 20.

State Key Laboratory of Pharmaceutical Biotechnology, Medical School and School of Life Science, and Jiangsu Key Laboratory for Nano Technology, Nanjing University, Nanjing, 210093, China.

In situ tumor vaccination is preliminarily pursued to strengthen antitumor immune response. Immunogenic tumor cell death spontaneously releases abundant antigens and adjuvants for activation of dendritic cells, providing a paragon opportunity for establishing efficient in situ vaccination. Herein, [email protected] nanosheets are constructed by integrating physcion (Phy, an inhibitor of the pentose phosphate pathway (PPP)) with layered gadolinium hydroxide (PLGdH) nanosheets to boost radiation-therapy (RT)-induced immunogenic cell death (ICD) for potent in situ tumor vaccination. It is first observed that sheet-like PLGdH can present superior X-ray deposition and tumor penetrability, exhibiting improved radiosensitization in vitro and in vivo. Moreover, the destruction of cellular nicotinamide adenine dinucleotide phosphate (NADPH) and nucleotide homeostasis by Phy-mediated PPP intervention can further amplify PLGdH-sensitized RT-mediated oxidative stress and DNA damage, which correspondingly results in effective ICD and enhance the immunogenicity of irradiated tumor cells. Consequently, [email protected] RT successfully primes robust CD8 -T-cell-dependent antitumor immunity to potentiate checkpoint blockade immunotherapies against primary and metastatic tumors.
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http://dx.doi.org/10.1002/adma.202109726DOI Listing
April 2022

Long-Life and High-Rate-Charging Lithium Metal Batteries Enabled by a Flexible Active Solid Electrolyte Interphase Layer.

ACS Appl Mater Interfaces 2021 Dec 8;13(50):60678-60688. Epub 2021 Dec 8.

Shanghai Key Laboratory of Materials Protection and Advanced Materials Electric Power, Shanghai Engineering Research Center of Energy-Saving in Heat Exchange Systems, Shanghai University of Electric Power, Shanghai 200090, P.R. China.

Commercially, lithium metal batteries are still limited by the growth of lithium dendrites and excessive consumption of the electrolyte. A stable multifunctional solid electrolyte interface is the development strategy of lithium metal batteries in the future. However, most of the artificial solid electrolyte interphases (SEIs) cannot meet the original intention of multifunctional design and cannot form an SEI film with a high conductivity and low nucleation potential. In this work, we report a universal and simple method of adding multifunctional fluorosulfonate to a commercial electrolyte, so increasing the inorganic LiF in the SEI. In addition, the imidazole ring in the fluorosulfonate combines with the alkyl group in the electrolyte to form a flexible interface layer, which inhibits the growth of lithium dendrites and makes lithium deposition more uniform, thereby realizing a stable fast charge cycle. With an ultralow capacity of 2 mAh/cm deposited, the symmetrical battery can be deposited stably for nearly 300 h at a high current density of 20 mA/cm. The capacity retention rate of the Li-LiFePO (LFP) full cell was still at 90.6% after 1000 cycles at 5 C. Even with 5 C high-rate fast charging, the capacity was maintained at 76.56% after 200 cycles, which is four times that of commercial electrolytes. This simple addition strategy gives insights into the practical application of the new electrolyte and provides a new idea for the construction of a stable SEI for commercial lithium metal batteries.
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http://dx.doi.org/10.1021/acsami.1c19952DOI Listing
December 2021

Harnessing superhydrophobic coatings for enhancing the surface corrosion resistance of magnesium alloys.

J Mater Chem B 2021 12 15;9(48):9893-9899. Epub 2021 Dec 15.

State and Local Joint Engineering Laboratory for Novel Functional Polymeric Materials, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, P. R. China.

Clinical applications of magnesium (Mg) alloys have been severely limited by their rapid corrosion in physiological environments. Superhydrophobic coatings can prevent water from contacting the Mg alloy surface, retarding alloy corrosion. In this study, a superhydrophobic Mg alloy was prepared by depositing fluorine-modified SiO nanoparticles (SiO-F NPs) with polydopamine (PDA). The dip-coating strategy presented is simple, green, can be undertaken without any complex equipment, has reduced power consumption, and decreased threat to human health. The as-prepared superhydrophobic Mg alloy is resistant to corrosion in simulated body fluid (SBF). The superhydrophobicity was stable after the tape, water-impact and sand-impact tests over multiple cycles. In addition, cytotoxicity investigations demonstrated that the superhydrophobic Mg alloy exhibited excellent cytocompatibility. This work opens up a new avenue in the design of superhydrophobic Mg alloys for corrosion resistance.
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http://dx.doi.org/10.1039/d1tb01974kDOI Listing
December 2021

Quasi-Isotropically Thermal Conductive, Highly Transparent, Insulating and Super-Flexible Polymer Films Achieved by Cross Linked 2D Hexagonal Boron Nitride Nanosheets.

Small 2021 Nov 11;17(46):e2101409. Epub 2021 Oct 11.

Key Laboratory of Science and Technology on Wear and Protection of Materials, Chinese Academy of Sciences, Lanzhou, 730000, P. R. China.

Polymer-based thermal management materials (TIMs) show great potentials as TIMs due to their excellent properties, such as high insulation, easy processing, and good flexibility. However, the limited thermal conductivity seriously hinders their practical applications in high heat generation devices. Herein, highly transparent, insulating, and super-flexible cellulose reinforced polyvinyl alcohol/nylon12 modified hexagonal boron nitride nanosheet (PVA/(CNC/PA-BNNS)) films with quasi-isotropic thermal conductivity are successfully fabricated through a vacuum filtration and subsequent self-assembly process. A special structure composed of horizontal stacked hexagonal boron nitride nanosheets (h-BNNSs) connected by their warping edges in longitudinal direction, which is strengthened by cellulose nanocrystals, is formed in PVA matrix during self-assembly process. This special structure makes the PVA/(CNC/PA-BNNS) films show excellent thermal conductivity with an in-plane thermal conductivity of 14.21 W m K and a through-plane thermal conductivity of 7.29 W m K . Additionally, the thermal conductive anisotropic constants of the as-obtained PVA/(CNC/PA-BNNS) films are in the range of 1 to 4 when the h-BNNS contents change from 0 to 60 wt%, exhibiting quasi-isotropic thermal conductivity. More importantly, the PVA/(CNC/PA-BNNS) films exhibit excellent transparency, super flexibility, outstanding mechanical strength, and electric insulation, making them very promising as TIMs for highly efficient heat dissipation of diverse electronic devices.
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http://dx.doi.org/10.1002/smll.202101409DOI Listing
November 2021

Zoledronic Acid-Gadolinium Coordination Polymer Nanorods for Improved Tumor Radioimmunotherapy by Synergetically Inducing Immunogenic Cell Death and Reprogramming the Immunosuppressive Microenvironment.

ACS Nano 2021 05 3;15(5):8450-8465. Epub 2021 May 3.

State Key Laboratory of Pharmaceutical Biotechnology, Medical School and School of Life Science, Nanjing University, Nanjing 210093, China.

Radiation therapy can potentially elicit a systemic immune response and cause the regression of nonirradiated tumors, and the checkpoint blockade immunotherapies have been introduced to improve their clinical response rate. However, the therapeutic benefits of radioimmunotherapy are still far from satisfactory. Herein, the self-assembled "carrier-free" coordination polymer nanorods are constructed based on gadolinium and zoledronic acid, which can deposit X-ray for improved reactive oxygen species production to induce potent immunogenic cell death (ICD), simultaneously deplete tumor-associated macrophages with regulatory cytokines inhibition, respectively. With the potent ICD induction and reprogrammed immunosuppressive microenvironment, this synergetic strategy can promote antigen presentation, immune priming and T-cell infiltration, and potentiate checkpoint blockade immunotherapies against primary, distant, and metastatic tumors.
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http://dx.doi.org/10.1021/acsnano.0c10764DOI Listing
May 2021

Index of microcirculatory resistance predicts long term cardiac systolic function in patients with STEMI undergoing primary PCI.

BMC Cardiovasc Disord 2021 02 2;21(1):66. Epub 2021 Feb 2.

Department of Cardiology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, 210008, China.

Background: To evaluate the predictive value of the index of microcirculatory resistance (IMR) for long-term cardiac systolic function after primary percutaneous coronary intervention (pPCI) in patients with acute anterior wall ST-segment elevation myocardial infarction (STEMI).

Methods: A total of 53 acute anterior wall STEMI patients were included and followed up within 1-year. IMR was measured to evaluate the immediate intraoperative reperfusion. IMR > 40 U was defined as the high IMR group and ≤ 40 U was defined as the low IMR group. Left ventricular ejection fraction (LVEF) was measured by echocardiography at 24 h, 1 month, 3 months, and 1 year after PCI to analyze the correlation between IMR and cardiac systolic function. Heart failure was estimated according to classification within one year.

Results: The ratio of TMPG (TIMI myocardial perfusion grade) 3 (85.7% vs. 52%, p = 0.015) and STR (ST-segment resolution) > 70% (82.1% vs. 48%, p = 0.019) were significantly higher in the low IMR group. The LVEF in the low IMR group was significantly higher than that in the high IMR group at 3 months (43.06 ± 2.63% vs. 40.20 ± 2.67%, p < 0.001) and 1 year (44.16 ± 2.40% vs. 40.13 ± 3.48%, p < 0.001). IMR was negatively correlated with LVEF at 3 months (r = - 0.1014, p = 0.0040) and 1 year (r = - 0.1754, p < 0.0001).

Conclusions: The IMR showed significant negative correlation with the LVEF value after primary PCI. The high IMR is a strong predictor of heart failure within 1 year after anterior myocardial infarction.
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http://dx.doi.org/10.1186/s12872-021-01887-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7852219PMC
February 2021

Genetic Diversity, Community Assembly, and Shaping Factors of Benthic Microbial Eukaryotes in Dongshan Bay, Southeast China.

Front Microbiol 2020 23;11:592489. Epub 2020 Dec 23.

State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, China.

Microbial eukaryotes are pivotal components of marine ecosystems. However, compared with the pelagic environments, the diversity distribution and the driving mechanisms of microbial eukaryotes in the marine sediments have rarely been explored. In this study, sediment cores were collected along a transect from inner to outer Dongshan Bay, Southeast China. By combining high throughput sequencing of small-subunit (SSU) rRNA gene with measurements on multiple environmental variables, the genetic diversity, community structure and assembly processes, and environmental shaping factors were investigated. Alveolata (mainly Ciliophora and Dinophyceae), Rhizaria (mainly Cercozoa), and Stramenopiles (mainly Bacillariophyta) were the most dominant groups in terms of both relative sequence abundance and operational taxonomic unit (OTU) richness. Grain size composition of the sediment was the primary factor determining the alpha diversity of microbial eukaryotes followed by sediment depth and heavy metal, including chromium (Cr), zinc (Zn), and plumbum (Pb). Geographic distance and water depth surpassed other environmental factors to be the primary factors shaping the microbial eukaryotic communities. Dispersal limitation was the primary driver of the microbial eukaryotic communities, followed by drift and homogeneous selection. Overall, our study shed new light on the spatial distribution patterns and controlling factors of benthic microbial eukaryotes in a subtropical bay which is subjected to increasing anthropogenic pressure.
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http://dx.doi.org/10.3389/fmicb.2020.592489DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7785585PMC
December 2020

Trophoblast Stem-Cell-Derived Exosomes Improve Doxorubicin-Induced Dilated Cardiomyopathy by Modulating the let-7i/YAP Pathway.

Mol Ther Nucleic Acids 2020 Dec 15;22:948-956. Epub 2020 Oct 15.

Department of Cardiology, Affiliated Drum Tower Hospital, Medical School of Nanjing University, Nanjing 21008, P.R. China.

Trophoblast stem cells (TSCs) have been confirmed to play a cardioprotective role in heart failure. However, whether TSC-derived exosomes (TSC-exos) can protect against cardiac injury remains unclear. In the present study, TSC-exos were isolated from the supernatant of TSCs using the ultracentrifugation method and characterized by transmission electron microscopy and western blotting. Utilizing the public Gene Expression Omnibus (GEO) database, we found that let-7i and Yes-associated protein 1 (YAP) could participate in the development of heart failure. , AC16 cardiomyocytes subjected to doxorubicin (DOX) were treated with TSC-exos or let-7i mimic. Flow cytometry showed that TSC-exos and let-7i both decreased cardiomyocyte apoptosis. , mice that were intraperitoneally injected into DOX received either PBS, TSC-exos, or AAV9-let7i for let-7i overexpression. Mice receiving TSC-exos and AAV9-let7i showed improved cardiac function and decreased inflammatory responses, accompanied by downregulated YAP signaling. Mechanistically, TSC-exos could transfer let-7i to cardiomyocytes and silence the YAP signaling pathway. In conclusion, TSC-exos could alleviate DOX-induced cardiac injury via the let-7i/YAP pathway, which sheds new light on the application of TSC-exos as a potential therapeutic tool for heart failure.
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http://dx.doi.org/10.1016/j.omtn.2020.10.014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7680701PMC
December 2020

Human trophoblast-derived exosomes attenuate doxorubicin-induced cardiac injury by regulating miR-200b and downstream Zeb1.

J Nanobiotechnology 2020 Nov 20;18(1):171. Epub 2020 Nov 20.

Department of Cardiology, Affiliated Drum Tower Hospital, Medical School of Nanjing University, Nanjing, 210008, Jiangsu, People's Republic of China.

Human trophoblast stem cells (TSCs) have been confirmed to play a cardioprotective role in heart failure. However, whether trophoblast stem cell-derived exosomes (TSC-Exos) can protect cardiomyocytes from doxorubicin (Dox)-induced injury remains unclear. In the present study, TSC-Exos were isolated from the supernatants of human trophoblasts using the ultracentrifugation method and characterized by transmission electron microscopy and western blotting. In vitro, primary cardiomyocytes were subjected to Dox and treated with TSC-Exos, miR-200b mimic or miR-200b inhibitor. Cellular apoptosis was observed by flow cytometry and immunoblotting. In vivo, mice were intraperitoneally injected into Dox to establish a heart failure model. Then, different groups of mice were administered either PBS, adeno-associated virus (AAV)-vector, AAV-miR-200b-inhibitor or TSC-Exos via tail vein injection. Then, the cardiac function, cardiac fibrosis and cardiomyocyte apoptosis in each group were evaluated, and the downstream molecular mechanism was explored. TSC-Exos and miR-200b inhibitor both decreased primary cardiomyocyte apoptosis. Similarly, mice receiving TSC-Exos and AAV-miR-200b inhibitor exhibited improved cardiac function, accompanied by reduced apoptosis and inflammation. The bioinformatic prediction and luciferase reporter results confirmed that Zeb1 was a downstream target of miR-200b and had an antiapoptotic effect. TSC-Exos attenuated doxorubicin-induced cardiac injury by playing antiapoptotic and anti-inflammatory roles. The underlying mechanism could be an increase in Zeb1 expression by the inhibition of miR-200b expression. In summary, this study sheds new light on the application of TSC-Exos as a potential therapeutic tool for heart failure.
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http://dx.doi.org/10.1186/s12951-020-00733-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7678329PMC
November 2020

Circulating exosomal miR-144-3p inhibits the mobilization of endothelial progenitor cells post myocardial infarction via regulating the MMP9 pathway.

Aging (Albany NY) 2020 08 25;12(16):16294-16303. Epub 2020 Aug 25.

Department of Cardiology, Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical University, Nanjing 210008, China.

Background: The angiogenesis post myocardial infarction (MI) is compromised in diabetes. MiR-144-3p is reported to be highly expressed in circulating exosomes of diabetic patients, implying its role in diabetic complications. However, whether circulating exosomes and enriched miR-144-3p are involved in the impaired neovascularization in diabetes and the underlying mechanism is unclear.

Results: DMexo and miR-144-3p mimic-treated MSCs had elevated miR-144-3p levels and decreased MMP9, Ets1 and PLG expression. The percentage of EPCs were relatively lower in DMexo-treated or agomir-treated MI mice compared with MI mice. Finally, the luciferase assay confirmed the direct binding between miR-144-3p and Ets1.

Conclusion: Exosomal miR-144-3p could impair the mobilization ability of EPCs, which was associated with impaired ischemia-induced neovascularization.

Methods: Circulating exosomes were isolated from Streptozotocin (STZ)-induced mice. , mesenchymal stem cells (MSCs) were incubated with exosomes from diabetic mice (DMexo), and miR-144-3p mimic or inhibitor. miR-144-3p, and MMP9 pathway were measured using qPCR and immunoblotting. , MI mice induced by left anterior descending ligation were treated with DMexo, as well as miR-144-3p agomir. Flow cytometry was used to profile endothelial progenitor cells (EPCs) in peripheral blood and bone marrow post 24 hours respectively.
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http://dx.doi.org/10.18632/aging.103651DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7485705PMC
August 2020

Comparison of the prognosis for different onset stage of cardiogenic shock secondary to ST-segment elevation myocardial infarction.

BMC Cardiovasc Disord 2020 06 19;20(1):302. Epub 2020 Jun 19.

Department of Cardiology, Drum Tower Hospital, Medical School of Nanjing University, Nanjing, 210008, China.

Objectives: The study was conducted to evaluate the outcomes of different onset stage of cardiogenic shock (CS) in the patients with ST-segment elevation myocardial infarction (STEMI).

Methods: Total 675 STEMI patients who had undergone primary percutaneous coronary intervention (pPCI) from November 2010 to December 2017 in Nanjing Drum Tower Hospital were enrolled. According to the onset time of CS, the cohort was divided into three groups: Non-CS group, CS on admission group and Developed CS group. The short-term (30 days), middle-term (12 months) and long-term (80 months) outcomes were analyzed. COX proportional hazard models were established for identification of the predictors.

Results: The all cause death, cardiac death and major adverse cardiac events (MACE) at 30 days were similar among the three groups. The incidence of MACE in the CS on admission group was significantly higher than the other two groups at 12 months. As to the long-term outcomes, the CS on admission group had lower survival rate than the other two groups. The Develop CS group had lower survival rate than Non-CS group numerically with a trend towards statistical significance. The incidence of cardiac death in the Non-CS group was the lowest. The incidence of MACE in the CS on admission group was much higher compared with the other two groups. After multivariate analysis, the independent predictors of all cause death included age, male sex, prior stroke and LVEF. The independent predictors of cardiac death included age, male sex, prior stroke, LVEF, CS on admission and developed CS. The independent predictors of MACE included age, prior stroke, LVEF, multivessel lesions, post-PCI TIMI grade 1 and CS on admission.

Conclusions: The long-term outcomes of CS on admission group were the worst of all. The outcomes of Developed CS group laid between the other two groups. The consequences highlighted the importance of prevention for CS developing in the STEMI patients during hospitalization.
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http://dx.doi.org/10.1186/s12872-020-01583-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7304156PMC
June 2020

PDK1-AKT signaling pathway regulates the expression and function of cardiac hyperpolarization-activated cyclic nucleotide-modulated channels.

Life Sci 2020 Jun 14;250:117546. Epub 2020 Mar 14.

Department of Cardiology, Nanjing University Medical School Affiliated Nanjing Drum Tower Hospital, Nanjing, China. Electronic address:

Aim: The enzyme 3-phosphoinositide-dependent protein kinase-1 (PDK1) is associated with cardiac and pathological remodeling and ion channel function regulation. However, whether it regulates hyperpolarization-activated cyclic nucleotide-modulated channels (HCNs) remains unclear.

Main Methods: In the atrial myocytes of heart-specific PDK1 "knockout" mouse model and neonatal mice, protein kinase B (AKT)-related inhibitors or agonists as well as knockdown or overexpression plasmids were used to study the relationship between PDK1 and HCNs.

Key Findings: HCN1 expression and AKT phosphorylation at the Thr308 site were significantly decreased in atrial myocytes after PDK1 knockout or inhibition; in contrast, HCN2 and HCN4 levels were significantly increased. Also, a similar trend of HCNs expression has been observed in cultured atrial myocytes after PDK1 inhibition, as further demonstrated via immunofluorescence and patch-clamp experiments. Moreover, these results of PDK1 overexpression indicate an opposite trend compared with the previous experimental results. However, the results of PDK1 inhibition or overexpression could be reversed by activating or inhibiting AKT, respectively.

Significance: These results indicate that the PDK1-AKT signaling pathway is involved in the regulation of HCN mRNA transcription, protein expression, HCN current density, and cell membrane location.
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http://dx.doi.org/10.1016/j.lfs.2020.117546DOI Listing
June 2020

Regenerated Cellulose Films with Amino-Terminated Hyperbranched Polyamic Anchored Nanosilver for Active Food Packaging.

ACS Appl Bio Mater 2020 Jan 23;3(1):602-610. Epub 2019 Dec 23.

Key Laboratory of Bio-Based Material Science & Technology, Northeast Forestry University, Ministry of Education, Harbin 150040, China.

The nanosilver-based antibacterial composite film used as food packaging has a potential hazard of silver leakage into the human body. In this study, hyperbranched polyamide-amine (HPAMAM) was used as a template, reducing agent, and stabilizer to synthesize Ag nanoparticles (Ag NPs) in situ, and then HPAMAM anchored Ag NPs onto oxidized cellulose to construct a regenerated cellulose film with a low silver leakage for antibacterial food packaging. Alkali hydroxide/urea solution was used to dissolve cotton fibers, and the hydroxyl groups at C-2 and C-3 of the glucose residues were oxidized to two aldehyde groups by NaIO. Then, HPAMAM/Ag NPs composites ([email protected] NPs) were grafted on the oxidized cellulose by the reaction between aldehyde groups and amino groups. The [email protected] NPs-embedded cellulose films were achieved by regenerating the dissolved cellulose with ethanol and drying naturally. With a low silver leakage (<10%), [email protected] NPs-embedded cellulose films exhibited a strong antibacterial effect on and and effectively extended the storage life of cherry tomatoes as food packaging. In addition, the physical properties of [email protected] NPs-embedded cellulose films were improved due to the anchor of Ag NPs onto oxidized cellulose by HPAMAM. This study provides a strategy for synthesizing [email protected] NPs-embedded cellulose film, which has the potential to be used as a biodegradable, renewable, and safe antibacterial food package material.
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http://dx.doi.org/10.1021/acsabm.9b00992DOI Listing
January 2020

The self-perceptions of aging were an important factor associated with the quality of life in Chinese elderly with hypertension.

Psychogeriatrics 2019 Jul 20;19(4):391-398. Epub 2019 Feb 20.

Division of Cardiology, The First Affiliated Hospital of Soochow University, Suzhou, China.

Background: In China, quality of life (QOL) of the elderly with hypertension was lower than that of normal elderly and lower self-perceptions of aging (SPA) in the elderly with chronic diseases have been shown. Previous studies showed that SPA played a key role in QOL of the elderly. We hypothesised that SPA were associated with QOL in Chinese elderly with hypertension, with more negative SPA predicting lower QOL.

Methods: A cross-sectional survey was conducted in one comprehensive hospital, 15 urban community clinics and 22 rural village clinics in Suzhou. In total, 1029 Chinese elderly with hypertension completed the self-administered questionnaires, including socio-demographic and clinical characteristics, aging perceptions questionnaire which contained seven dimensions and Medical Outcomes Study 36-item Short Form Health Survey evaluating QOL which included physical component summary (PCS) and mental component summary (MCS).

Results: Correlation analysis between SPA and QOL showed that most dimensions of SPA were correlated with PCS and MCS, except for Emotional Representations not correlated with MCS. The results of multivariate logistic regression analysis showed: the dimensions Timeline Chronic (B = -2.651, P < 0.001), Consequences Negative (B = -2.155, P < 0.001) and Emotional Representations (B = -2.672, P < 0.001) had a negative association with PCS, while Timeline Cyclical (B = 1.401, P = 0.023), Consequences Positive (B = 1.200, P = 0.011) and Control Positive (B = 1.425, P = 0.004) had a positive association with PCS; the dimensions Timeline Cyclical (B = -1.734, P < 0.001) and Consequences Negative (B = -1.646, P < 0.001) had a negative association with MCS, while Timeline Chronic (B = 1.313, P < 0.001), Consequences Positive (B = 0.962, P = 0.012), Control Positive (B = 2.453, P < 0.001) and Control Negative (B = 1.860, P < 0.001) had a positive association with MCS.

Conclusions: Our results suggest that negative SPA tended to be associated with lower QOL in Chinese elderly with hypertension. Some measures to improve unhealthy negative SPA should be delivered to improve QOL in Chinese elderly with hypertension.
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http://dx.doi.org/10.1111/psyg.12400DOI Listing
July 2019

Combined Primary PCI with Multiple Thrombus Burden Reduction Therapy Improved Cardiac Function in Patients with Acute Anterior Myocardial Infarction.

Int Heart J 2019 Jan 20;60(1):27-36. Epub 2018 Nov 20.

Key laboratory of Cardiovascular & Cerebrovascular Medicine, School of Pharmacy, Nanjing Medical University.

High thrombus burden induced slow-flow and no-reflow during primary percutaneous coronary intervention (PCI) and is associated with a poor prognosis. We aimed to investigate whether a combined thrombus burden reduction therapy during primary PCI, could improve microcirculation and enhance cardiac function in the long-term.Anterior wall STEMI patients with high thrombus burden were randomly assigned to receive a combined thrombus burden reduction therapy or thrombus aspiration alone. The primary end points included the percentage of patients with TMPG (TIMI myocardial perfusion grade) 3, STR (ST-segment resolution) above 70%, the index of microcirculatory resistance (IMR) and left ventricular ejection fraction (LVEF) difference.Twenty-two patients in the combined interventional group and 24 in the control group completed 1-year follow-up. The percentages of patients with TMPG 3 (68.2% versus 33.3%, P = 0.006) and STR above 70% (63.6% versus 25%, P = 0.016) were significantly higher in the combined group. IMR was significantly lower in the combined interventional group (31.50 ± 13.39 U versus 62.72 ± 22.80 U, P = 0.002). At 3 months and 1 year, the overall LVEF value was better in the combined interventional group (42.1% versus 40.0%, P = 0.049; 41.9% versus 39.8%, P = 0.042), respectively. The IMR value was negatively correlated with the EF value at 3 months (r = -0.145, P = 0.013) and 1 year (r = -0.333, P = 0.031).A combined thrombus burden reduction therapy during primary PCI can safely reduce thrombus burden, improve myocardial tissue perfusion, and improve cardiac function among STEMI patients with high thrombus burden. IMR might be a good predictor for post-myocardial infarction cardiac function.
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http://dx.doi.org/10.1536/ihj.18-064DOI Listing
January 2019

Adsorption behavior of Ni(II) onto activated carbons from hide waste and high-pressure steaming hide waste.

Ecotoxicol Environ Saf 2018 Jul 20;156:294-300. Epub 2018 Mar 20.

Shandong Provincial Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Jinan 250100, China. Electronic address:

This work reports the preparation and adsorption of Ni(II) via activated carbons which produced from hide waste (HWAC) and high-pressure steaming hide waste (HWSAC) with potassium silicate as the activating agent. The best preparation condition for HWAC and HWSAC was the activation temperature of 700 °C using an impregnation ratio of 2:1. Both of them were characterized by N adsorption/desorption isotherms, SEM and FT-IR spectra. The surface area of HWAC and HWSAC was 1804.37 and 1361.26 m/g, respectively. Despite the surface area of HWAC being larger than that of HWSAC, but the adsorption capacity of Ni(II) for HWAC was lower than that for HWSAC. Furthermore, the adsorption capacity of Ni(II) for both HWAC and HWSAC showed pH-dependent behavior and increased with the increase in pH value, which can be attributed to the functional groups of HWAC and HWSAC materials through the electrostatic attraction. The adsorption data for HWAC and HWSAC were fitted with four isotherm models (Langmuir, Freundlich, Temkin, and Dubinin-Radushkevich) and four kinetic models (pseudo-first order model, pseudo-second order model, intra-particle diffusion and Elovich equation), indicating that Langmuir isotherm and Pseudo-second order model fitted well with high coefficient of determination (R > 0.99) for both the two adsorbents. The positive enthalpy of adsorption (ΔH) and free energy of adsorption (ΔG) indicate a spontaneous and endothermic nature of the process. These results demonstrated that activated carbon can be prepared from hide waste which could remove heavy metal such as Ni(II) effectively.
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http://dx.doi.org/10.1016/j.ecoenv.2018.03.017DOI Listing
July 2018

Expression of integrin-linked kinase improves cardiac function in a swine model of myocardial infarction.

Exp Ther Med 2017 May 23;13(5):1868-1874. Epub 2017 Feb 23.

Department of Cardiology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, Jiangsu 210008, P.R. China.

Previous studies have described the beneficial effects of overexpressing integrin-linked kinase (ILK) after myocardial infarction (MI) in small animal models. However, the effects of ILK in pre-clinical large animals are not known. To move closer to clinical translation, we examined the effects of ILK gene transfer in a swine model of ischemic heart disease. Swine received percutaneous intracoronary injections of adenoviral vector expressing ILK (n=10) or empty ad-null (n=10) in the left anterior descending coronary artery (LAD) following LAD occlusion. Four weeks after transfection, we confirmed that transgene expression was restricted to the infarcted area in the cardiac tissue. Imaging studies demonstrated preserved cardiac function in the ILK group. ILK treatment was associated with reduced infarcted scar size and preserved left ventricular (LV) geometry (LV diameter and LV wall thickness). Enhanced angiogenesis was preserved in the ILK animals, along with reduction of apoptosis. ILK gene therapy improves cardiac remodeling and function in swine following MI associated with increased angiogenesis, reduced apoptosis, and increased cardiomyocyte proliferation with no signs of toxicity. These results may deliver a new approach to treat post-infarct remodeling and subsequent heart failure.
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http://dx.doi.org/10.3892/etm.2017.4162DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5443207PMC
May 2017

[Clinical features of acute poisoning in hospitalized children: an analysis of 586 cases].

Zhongguo Dang Dai Er Ke Za Zhi 2017 Apr;19(4):441-445

Department of Pharmacy/Ministry of Education Key Laboratory of Child Development and Disorders/China International Science and Technology Cooperation Base of Child Development and Critical Disorders/Chongqing Key Laboratory of Pediatrics, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.

Objective: To investigate the clinical features of acute poisoning in hospitalized children.

Methods: A retrospective analysis was performed on the clinical data of 586 hospitalized children who were diagnosed with poisoning and discharged from the Children's Hospital of Chongqing Medical University between January 2006 and December 2015.

Results: The patients included 354 males and 232 females (age: 24 days to 15.8 years). Of the 586 cases, 450 (76.8%) were infants and preschool children; 463 (79.0%) came from rural areas; 551 (94.0%) were hospitalized because of unintentional poisoning. The drug poisoning, pesticide poisoning, and rodenticide poisoning accounted for 221 cases (37.7%), 167 cases (28.5%), and 175 cases (29.9%) respectively. There was a significant difference in the distribution of the poisoning toxins between urban and rural children (P<0.01), and drugs and pesticides were the most common toxins for urban and rural children respectively. There were significant differences in main clinical manifestations between the children with drug poisoning, pesticide poisoning, and rodenticide poisoning (P<0.01), who presented with main clinical symptoms of the nervous system, digestive system, and circulatory system respectively. There was no significant difference in overall response rate between the children poisoned by different toxins.

Conclusions: Acute poisoning is most common in infants and preschool children. The majority of the patients are from rural areas. The majority of acute poisoning is unintentional. Poisoning by drugs is the main type of acute poisoning. There is no significant difference in overall response rate between the children poisoned by different toxins, but their clinical manifestations are different.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7389664PMC
April 2017

Syndecan-4 deficiency accelerates the transition from compensated hypertrophy to heart failure following pressure overload.

Cardiovasc Pathol 2017 May - Jun;28:74-79. Epub 2017 Mar 30.

Department of Cardiology, Affiliated Drum Tower Hospital, Nanjing University Medical School, Nanjing, China. Electronic address:

Increasing evidence suggests that a mismatch between angiogenesis and myocardial growth contributes to the transition from adaptive cardiac hypertrophy to heart failure following pressure overload. Syndecan-4 is a transmembrane proteoglycan that binds to growth factors and extracellular matrix proteins and is critical in focal adhesion formation. However, its effects on coronary angiogenesis during pressure overload-induced heart failure have not been studied. Here, we hypothesize that syndecan-4 modulates cardiac remodeling in response to pressure overload through its ability to regulate adaptive angiogenesis. Syndecan-4 knockout (syndecan-4 KO) and wild-type (WT) mice were subjected to pressure overload induced by transverse aortic constriction (TAC). Syndecan-4 KO mice exhibited reduced capillary density, attenuated cardiomyocyte size, and worsened left ventricular cardiac function after TAC surgery compared with WT mice. Moreover, syndecan-4 KO mice showed a significant decrease in protein kinase C alpha expression. Our data suggest that syndecan-4 is essential for the compensated hypertrophy and the maintenance of cardiac function during the process of heart failure following pressure overload.
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http://dx.doi.org/10.1016/j.carpath.2017.03.008DOI Listing
December 2017

Cardiac repair in a mouse model of acute myocardial infarction with trophoblast stem cells.

Sci Rep 2017 03 15;7:44376. Epub 2017 Mar 15.

Department of Cardiology, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School, Nanjing, Jiangsu, 210008, China.

Various stem cells have been explored for the purpose of cardiac repair. However, any individual stem cell population has not been considered as the ideal source. Recently, trophoblast stem cells (TSCs), a newly described stem cell type, have demonstrated extensive plasticity. The present study evaluated the therapeutic effect of TSCs transplantation for heart regeneration in a mouse model of myocardial infarction (MI) and made a direct comparison with the most commonly used mesenchymal stem cells (MSCs). Transplantation of TSCs and MSCs led to a remarkably improved cardiac function in contrast with the PBS control, but only the TSCs exhibited the potential of differentiation into cardiomyocytes in vivo. In addition, a significantly high proliferation level of both transplanted stem cells and resident cardiomyocytes was observed in the TSCs group. These findings primary revealed the therapeutic potential of TSCs in transplantation therapy for MI.
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http://dx.doi.org/10.1038/srep44376DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5353648PMC
March 2017

[Association between use of antibacterial agents in the first year of life and childhood asthma: a Meta analysis].

Zhongguo Dang Dai Er Ke Za Zhi 2016 Oct;18(10):995-1000

Department of Pharmacy, Children's Hospital of Chongqing Medical University/Ministry of Education Key Laboratory of Child Development and Disorders/China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing 400014, China.

Objective: To evaluate the association between the use of antibacterial agents in the first years of life and childhood asthma.

Methods: The Chinese and English databases CNKI, Wanfang Data, VIP, PubMed, and EBSCO were searched for prospective cohort studies on the association between the use of antibacterial agents in the first years of life and childhood asthma. Stata12.0 software was used to analyze the association through a Meta analysis.

Results: The articles with a high quality score and adjusted effective values for factors for lower respiratory tract infection were pooled, and a total of 8 studies were included. The results of the Meta analysis showed that the use of antibacterial agents in the first years of life increased the risk of childhood asthma (OR=1.14, 95%CI: 1.10-1.17, P<0.05). Compared with the children who used antibacterial agents 0-1 times in the first years of life, those who used more than 4 times had an increased risk of asthma (OR=1.28, 95%CI: 1.19-1.38, P<0.05). High-risk children (at least one immediate family member had asthma) who used antibacterial agents had an increased risk of asthma (OR=1.47, 95%CI: 1.20-1.81, P<0.05).

Conclusions: The use of antibacterial agents in the first years of life increases the risk of childhood asthma. High-risk children who use antibacterial agents have an increased risk of asthma. The increased frequency of use of antibacterial agents in the first years of life is associated with an increased risk of childhood asthma, but the detailed dose relationship needs further investigation.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7389538PMC
October 2016
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