Publications by authors named "Rong Chen"

1,312 Publications

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Detection of functional and structural brain alterations in female schizophrenia using elastic net logistic regression.

Brain Imaging Behav 2021 Jul 27. Epub 2021 Jul 27.

Neuropsychiatry Imaging Center, Shenzhen Mental Health Center, Shenzhen, China.

Neuroimaging technique is a powerful tool to characterize the abnormality of brain networks in schizophrenia. However, the neurophysiological substrate of schizophrenia is still unclear. Here we investigated the patterns of brain functional and structural changes in female patients with schizophrenia using elastic net logistic regression analysis of resting-state functional magnetic resonance imaging data. Data from 52 participants (25 female schizophrenia patients and 27 healthy controls) were obtained. Using an elastic net penalty, the brain regions most relevant to schizophrenia pathology were defined in the models using the amplitude of low-frequency fluctuations (ALFF) and gray matter, respectively. The receiver operating characteristic analysis showed reliable classification accuracy with 85.7% in ALFF analysis, and 77.1% in gray matter analysis. Notably, our results showed eight common regions between the ALFF and gray matter analyses, including the Frontal-Inf-Orb-R, Rolandic-Oper-R, Olfactory-R, Angular-L, Precuneus-L, Precuenus-R, Heschl-L, and Temporal-Pole-Mid-R. In addition, the severity of symptoms was found positively associated with the ALFF within the Rolandic-Oper-R and Frontal-Inf-Orb-R. Our findings indicated that elastic net logistic regression could be a useful tool to identify the characteristics of schizophrenia -related brain deterioration, which provides novel insights into schizophrenia diagnosis and prediction.
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http://dx.doi.org/10.1007/s11682-021-00501-zDOI Listing
July 2021

The effects of early enteral nutrition on the nutritional statuses, gastrointestinal functions, and inflammatory responses of gastrointestinal tumor patients.

Am J Transl Res 2021 15;13(6):6260-6269. Epub 2021 Jun 15.

Clinical Nutrition Division, Wujin Hospital Affiliated With Jiangsu University Changzhou 213017, Jiangsu Province, China.

Objective: This paper was designed to investigate the effects of early enteral nutrition (EEN) on the nutritional statuses, gastrointestinal functions, and inflammatory responses of gastrointestinal tumor patients.

Methods: A total of 194 gastrointestinal tumor patients treated in our hospital from May 2017 to February 2019 were recruited as the study cohort. Among them, 101 patients were administered enteral nutrition (the study group), and 93 patients were administered parenteral nutrition (the control group). The two groups were compared in terms of their nutritional statuses, gastrointestinal functions, inflammatory responses, and other indicators.

Results: On the third day after the operation (postoperative 3 d), the serum albumin (ALB) and prealbumin (PA) levels were significantly reduced in both groups (P>0.05). On the seventh day after the operation (postoperative 7 d), the two nutritional indices increased significantly in both groups (P<0.05), and were significantly higher in the study group (P<0.05). Compared with the control group, the patients in the study group experienced shorter lengths of stay (LOS), earlier first anal exhaust times, and faster intestinal peristalsis recovery times (P<0.05). On the third day after the operations, the high-sensitive C-reactive protein (hs-CRP) and prostaglandin E (PGE) levels were significantly reduced in both groups (P<0.05), and the decrease was significantly greater in the study group (P<0.05). In addition, the CD3, CD4, CD8 and CD4/CD8 levels were significantly reduced in both groups on the third day after the operation (P>0.05). On the seventh day after the operation, the first three immune indices increased significantly in both groups (P<0.05), and they were significantly higher in the study group (P<0.05). The incidences of vomiting, diarrhea, and abdominal distension in the study group were significantly lower than they were in the control group (P<0.05). After the treatment, the patients' quality of life (QOL) was significantly higher in the study group (P<0.05).

Conclusion: For gastrointestinal tumor patients, EEN can improve their gastrointestinal functions, enhance their immune functions, and reduce their expressions of inflammatory cytokines while improving their nutritional statuses and QOL.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8290805PMC
June 2021

A Dissipation Function-Based Method for Calculating the Energy Loss of Intracranial Aneurysms.

Front Neurol 2021 8;12:639690. Epub 2021 Jul 8.

Beijing Key Laboratory of Fundamental Research on Biomechanics in Clinical Application, School of Biomedical Engineering, Capital Medical University, Beijing, China.

At present, the energy loss (EL) mechanism of intracranial aneurysm (IA) rupture is explored based on the global EL calculated by Bernoulli equation, but the details of EL are still unclear. This study aimed to explore the temporal and spatial characteristics of EL of IAs and reveal its mechanism. A novel method for calculating the EL of IAs based on dissipation function (DF) was proposed. DF was derived from the differential form of the energy equation and reflected the irreversible conversion from mechanical energy to internal energy caused by the friction between the fluid micelles. Eight sidewall IAs located at the posterior communicating segment of the internal carotid artery were collected; the three-dimensional (3D) geometric models of IAs were established employing image segmentation and 3D reconstruction. Computational fluid dynamics was applied to obtain hemodynamic parameters of IAs. The temporal and spatial characteristics of EL of IAs were achieved utilizing our proposed method. The simulation results indicated that EL occurred mainly in the boundary layer and the region adjacent to high-velocity inflow jet, EL increased rapidly during cardiac systole and reached its maximum at end-systolic phase and then decreased gradually during diastole until the end of cardiac cycle. The proposed method achieved some improvements over the traditional Bernoulli equation-based method by acquiring the temporal and spatial characteristics of EL, and it could provide insights into the EL of IAs and contribute to further rupture mechanism investigation.
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http://dx.doi.org/10.3389/fneur.2021.639690DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8296906PMC
July 2021

Field to laboratory comparison of metal accumulation on aged microplastics in coastal waters.

Sci Total Environ 2021 Jul 21;797:149108. Epub 2021 Jul 21.

State Key Laboratory of Marine Environmental Science and College of the Environment and Ecology, Xiamen University, Xiamen, Fujian 361102, China; Key Laboratory of the Coastal and Wetland Ecosystems of Ministry of Education, Xiamen, Fujian 361102, China; Center for Marine Environmental Chemistry and Toxicology, Xiamen University, Xiamen, Fujian 361102, China. Electronic address:

The ubiquity of microplastics in the environment has attracted much attention on their risks. Though newly produced plastics were considered inert to aqueous metals, a few studies suggest aged microplastics can accumulate metals. Still, knowledge gap exists on the comparability of metal accumulation in field condition and that acquired in controlled laboratory settings. Accordingly, we comparatively assessed the field accumulation and laboratory adsorption of metals on aged microplastics in coastal waters. Microplastics of different polymeric types were aged for 8 weeks at three coastal sites with different contamination levels. Microplastics accumulated metals to substantial concentrations during ageing (median concentrations, μg g: Fe = 950, Mn = 94, Zn = 19, Cu = 2.8, Ni = 1.7, Pb = 1.6, and Cd = 0.005). Adsorption capacity of (aged) microplastics was evaluated in laboratory using a stable isotope tracer method. At environmentally realistic concentrations (μg L, Cd = 1.7, Cu = 4.4, Ni = 5.4, Pb = 0.5, and Zn = 13), the median concentrations of newly adsorbed isotopes on the aged microplastics were 0.01, 1.4, 0.07, 0.56, and 1.1 μg g, respectively, one to two orders of magnitude higher than those adsorbed on pristine microplastics. However, the composition pattern of metals accumulated on aged microplastics differed from the composition of metals newly adsorbed in laboratory: the prior one reflected the contamination status of ageing sites and varied by polymeric types; whereas the laboratory newly adsorbed metals on aged microplastics were uniformly correlated to particulate Fe and Mn concentrations, suggesting Fe and Mn mineral coatings mediated the ensuing metal adsorption. Such discrepancy unveiled the complexity of metal accumulation behavior in the real environment and highlighted that cares should be taken when translating laboratory findings to risk assessment of metal contaminated microplastics in the real environment.
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http://dx.doi.org/10.1016/j.scitotenv.2021.149108DOI Listing
July 2021

Highly Luminescent Zero-Dimensional Organic Copper Halides for X-ray Scintillation.

J Phys Chem Lett 2021 Jul 20;12(29):6919-6926. Epub 2021 Jul 20.

School of Optical and Electronic Information, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.

The present work reports highly efficient flexible and reabsorption-free scintillators based on two zero-dimensional (0D) organic copper halides (TBA)CuX (TBA = tetrabutylammonium cation; X = Cl, Br). The (TBA)CuX exhibit highly luminescent green and sky-blue emissions peaked at 510 and 498 nm, with large Stokes shifts of 224 and 209 nm and high photoluminescence quantum yields (PLQYs) of 92.8% and 80.5% at room temperature for (TBA)CuCl and (TBA)CuBr single crystals (SCs), respectively. Interestingly, above room temperature, their PLQYs increase with temperature and reach near unity at 320 and 345 K for (TBA)CuCl and (TBA)CuBr, respectively. The excellent properties originate from self-trapped excitons (STEs) in individual [CuX] quantum rods, which is demonstrated by the temperature-dependent PL, ultrafast transient absorption (TA) combined with density functional theory (DFT) calculations. The (TBA)CuX scintillators show bright radioluminescence (RL), impressive linear response to dose rate in a broad range, and high light yields. Their potential application in X-ray imaging is demonstrated by using (TBA)CuX composite scintillation screens. Importantly, flexible scintillators are demonstrated to be superior than flat ones for imaging nonplanar objects by conformally coating, which produce accurate images with negligible distortion.
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http://dx.doi.org/10.1021/acs.jpclett.1c01946DOI Listing
July 2021

Long-Residence Pneumonia Vaccine Developed Using PEG-Grafted Hybrid Nanovesicles from Cell Membrane Fusion of Mycoplasma and IFN-γ-Primed Macrophages.

Small 2021 Jul 16:e2101183. Epub 2021 Jul 16.

School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, 210009, China.

CD8 T cell responses play a critical regulatory role in protection against mycoplasma infection-related respiratory diseases. Nanovesicles derived from cell membranes have been shown to induce CD8 T cell responses. Moreover, the short residence time of mycoplasma membrane-related vaccines in local lymph nodes limits the efficacy of current mycoplasma vaccines. Here, a long-residence pneumonia vaccine is developed using nanovesicles prepared by cell membrane fusion of Mycoplasma hyopneumoniae and interferon-γ (IFN-γ   )-primed macrophages, which are grafted with polyethylene glycol to increase residence time in the lymph nodes. Upregulation of intercellular adhesion molecule-1 (ICAM-1) on the membrane of IFN-γ-primed macrophages increases the targeting of the hybrid nanovesicle vaccine to the local lymph nodes, with increased CD8 T cell activation. A mechanistic study reveals that CD8 T cell activation is achieved via a pathway involving upregulation of C-C motif chemokine ligand 2/3 expression by E26 transformation-specific sequences, followed by increased immune-stimulatory activity of dendritic cells. In vivo, prophylactic testing reveals that the hybrid nanovesicle vaccine triggers a long-term immune response, as evidenced by a memory CD8 T cell response against mycoplasma infection. The current study provides a new design strategy for mycoplasma vaccines that involves a hybrid method using biological sources and artificial modification.
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http://dx.doi.org/10.1002/smll.202101183DOI Listing
July 2021

Identification of Potential Key Genes and Regulatory Markers in Essential Thrombocythemia Through Integrated Bioinformatics Analysis and Clinical Validation.

Pharmgenomics Pers Med 2021 5;14:767-784. Epub 2021 Jul 5.

Department of Hematology, First Affiliated Hospital of Xinjiang Medical University, Urumqi, 830011, People's Republic of China.

Introduction: Essential thrombocytosis (ET) is a group of myeloproliferative neoplasms characterized by abnormal proliferation of platelet and megakaryocytes. Research on potential key genes and novel regulatory markers in essential thrombocythemia (ET) is still limited.

Methods: Downloading array profiles from the Gene Expression Omnibus database, we identified the differentially expressed genes (DEGs) through comprehensive bioinformatic analysis. GO, and REACTOME pathway enrichment analysis was used to predict the potential functions of DEGs. Besides, constructing a protein-protein interaction (PPI) network through the STRING database, we validated the expression level of hub genes in an independent cohort of ET, and the transcription factors (TFs) were detected in the regulatory networks of TFs and DEGs. And the candidate drugs that are targeting hub genes were identified using the DGIdb database.

Results: We identified 63 overlap DEGs that included 21 common up-regulated and 42 common down-regulated genes from two datasets. Functional enrichment analysis shows that the DEGs are mainly enriched in the immune system and inflammatory processes. Through PPI network analysis, , and were selected as hub genes. Interestingly, we found that the dysregulated hub genes are also aberrantly expressed in a bone marrow cohort of ET. Moreover, we found that the expression of , and genes were significantly under-expressed in ET (<0.05), which is consistent with our bioinformatics analysis. The ROC curve analysis also shows that these hub genes have good diagnostic value. Besides, we identified 4 TFs (SPI1, IRF4, SRF, and AR) as master transcriptional regulators that were associated with regulating the DEGs in ET. Cyclophosphamide, prednisone, fluorouracil, ruxolitinib, and lenalidomide were predicted as potential candidate drugs for the treatment of ET.

Discussion: These dysregulated genes and predicted key regulators had a significant relationship with the occurrence of ET with affecting the immune system and inflammation of the processes. Some of the immunomodulatory drugs have potential value by targeting , and genes in the treatment of ET.
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http://dx.doi.org/10.2147/PGPM.S309166DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275175PMC
July 2021

Systematic mining of fungal chimeric terpene synthases using an efficient precursor-providing yeast chassis.

Proc Natl Acad Sci U S A 2021 Jul;118(29)

Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, Wuhan University School of Pharmaceutical Sciences, Wuhan 430071, People's Republic of China;

Chimeric terpene synthases, which consist of C-terminal prenyltransferase (PT) and N-terminal class I terpene synthase (TS) domains (termed PTTSs here), is unique to fungi and produces structurally diverse di- and sesterterpenes. Prior to this study, 20 PTTSs had been functionally characterized. Our understanding of the origin and functional evolution of genes is limited. Our systematic search of sequenced fungal genomes among diverse taxa revealed that genes were restricted to Dikarya. Phylogenetic findings indicated different potential models of the origin and evolution of genes. One was that genes originated in the common Dikarya ancestor and then underwent frequent gene loss among various subsequent lineages. To understand their functional evolution, we selected 74 genes for biochemical characterization in an efficient precursor-providing yeast system employing chassis-based, robot-assisted, high-throughput automatic assembly. We found 34 genes that encoded active enzymes and collectively produced 24 di- and sesterterpenes. About half of these di- and sesterterpenes were also the products of the 20 known PTTSs, indicating functional conservation, whereas the PTTS products included the previously unknown sesterterpenes, sesterevisene (1), and sesterorbiculene (2), suggesting that a diversity of PTTS products awaits discovery. Separating functional PTTSs into two monophyletic groups implied that an early gene duplication event occurred during the evolution of the PTTS family followed by functional divergence with the characteristics of distinct cyclization mechanisms.
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http://dx.doi.org/10.1073/pnas.2023247118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8307374PMC
July 2021

Dielectric constants of organic pollutants determine their strength for enhancing microbial iron reduction.

Environ Sci Pollut Res Int 2021 Jul 12. Epub 2021 Jul 12.

State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, 430078, People's Republic of China.

Physicochemical properties are essential characteristics of organic compounds, which not only impact the fate of organic pollutants but also determine their application in biological processes. Here, we first found that the dielectric constants (ɛ) of organic pollutants negatively correlated to their strength for enhancing microbial Fe(III) reduction. Those with lower ɛ values than 2.61 potentially promoted the above process following the sequence carbon tetrachloride (CT) > benzene > toluene > tetrachloroethylene (PCE) due to their different ability to deprotonate the phosphorus-related groups on the outer cell membrane of iron-reducing bacteria Shewanella oneidensis MR-1 (MR-1). The stronger deprotonation of phosphorus-related groups induced more negative charge of cell surface and more strongly increased cell membrane permeability and consequently stimulated faster release of flavin mononucleotide (FMN) as an electron shuttle/cofactor for Fe(III) reduction. These findings are significant for understanding the biogeochemistry in multi-organic contaminated subsurface and providing knowledge for remediation strategies and current production.
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http://dx.doi.org/10.1007/s11356-021-14060-9DOI Listing
July 2021

Transcriptome sequencing and functional characterization of new sesquiterpene synthases from Curcuma wenyujin.

Arch Biochem Biophys 2021 Sep 9;709:108986. Epub 2021 Jul 9.

Key Laboratory of Elemene Class Anti-cancer Chinese Medicine of Zhejiang Province, Engineering Laboratory of Development and Application of Traditional Chinese Medicine from Zhejiang Province, Holistic Integrative Pharmacy Institute, Health Science Center, Hangzhou Normal University, Hangzhou, Zhejiang, 311121, China. Electronic address:

Tubers of Curcuma wenyujin are rich in essential oils, mainly various sesquiterpenes, showing antibacterial, anti-viral and anti-tumor effects. However, the molecular mechanism of C. wenyujin is deficient and related sesquiterpene synthases are still unclear. In this study, the transcriptome data of tubers and leaves from C. wenyujin were obtained and assembled into 78 092 unigenes. Of them, 244 unigenes were predicted to be involved in terpenoid biosynthesis while 131 unigenes were categorized as the "Terpenoid backbone biosynthesis" (TBB) term. Twenty-two unigenes possessed terpene synthase domain; five were predicted to be sesquiterpene synthases. Of the 208 unigenes annotated as cytochromes P450, 8 unigenes with full-length coding sequences were part of the CYP71 clade that primarily may perform hydroxylations of specialized metabolites. Furthermore, Ten DEGs related to the C5 precursor supply and sesquiterpene synthesis were validated by Real-time PCR; that showed a close correspondence with transcriptome sequence. A novel germacrene B synthase (CwGBS) and α-santalene synthase (CwSS) were identified in metabolically engineering E. coli. This study provided the first de novo transcriptome comparative analysis of leaf and tuber tissues from C. wenyujin, aiming to understand genetic mechanisms. Key genes involved in the biosynthesis of sesquiterpene will help for revealing the underlying mechanisms of C. wenyujin.
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http://dx.doi.org/10.1016/j.abb.2021.108986DOI Listing
September 2021

Research on a modularized long pulse generator based on anti-resonance network and transmission line transformer.

Rev Sci Instrum 2021 Apr;92(4):044702

College of Advanced Interdisciplinary Studies, National University of Defense Technology, Changsha 410073, China.

A modularized generator with three long pulse modules is designed and constructed in this work. The long pulse module consists of a three-section anti-resonance network and a three-stage transmission line transformer. A single module can output a pulse with an amplitude of 33 kV and a full width at half maximum (FWHM) of 400 ns, when the primary energy storage system provides a voltage of 24 kV. After the three modules are connected in series in experiment, the modularized long pulse generator delivers a high-voltage pulse to the matched load resistor with a maximum voltage of 96 kV and an FWHM of 400 ns. Repetitive experiments at a repetition frequency of 10 Hz are also carried out.
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http://dx.doi.org/10.1063/5.0039673DOI Listing
April 2021

A durable microsecond solid-state pulsed power system.

Rev Sci Instrum 2021 May;92(5):054707

College of Advanced Interdisciplinary Studies, National University of Defense Technology, Changsha 410073, China.

An all-solid-state microsecond pulsed power system has been tested in this paper. It can produce larger than 50 kV and microsecond-range pulses continuously for more than 9 × 10 shots into a resistive load at a repetition rate of 10 pps. The whole device has accumulatively operated more than 15 000 pulses at 100 Hz and 3 × 10 pulses at 10 Hz. This all-solid-state pulsed power system consists of a repetitive power supply, a four-stage Marx generator, a pulse forming network, and a resistive load. The repetitive power supply of the pulsed power system is calculated and analyzed first, followed by the introduction of the Marx generator and the three-section anti-resonance network. A polymer box of sodium chloride solution is applied as a resistive load with an impedance of 200 Ω. Repetitive experiments showed that this system is able to operate stably at 100 Hz repetition rate without failure.
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http://dx.doi.org/10.1063/5.0049795DOI Listing
May 2021

Reversing neural circuit and behavior deficit in mice exposed to maternal inflammation by Zika␣virus.

EMBO Rep 2021 Jul 7:e51978. Epub 2021 Jul 7.

Center for Craniofacial Molecular Biology, University of Southern California (USC), Los Angeles, CA, USA.

Zika virus (ZIKV) infection during pregnancy is linked to various developmental brain disorders. Infants who are asymptomatic at birth might have postnatal neurocognitive complications. However, animal models recapitulating these neurocognitive phenotypes are lacking, and the circuit mechanism underlying behavioral abnormalities is unknown. Here, we show that ZIKV infection during mouse pregnancy induces maternal immune activation (MIA) and leads to autistic-like behaviors including repetitive self-grooming and impaired social memory in offspring. In the medial prefrontal cortex (mPFC), ZIKV-affected offspring mice exhibit excitation and inhibition imbalance and increased cortical activity. This could be explained by dysregulation of inhibitory neurons and synapses, and elevated neural activity input from mPFC-projecting ventral hippocampus (vHIP) neurons. We find structure alterations in the synaptic connections and pattern of vHIP innervation of mPFC neurons, leading to hyperconnectivity of the vHIP-mPFC pathway. Decreasing the activity of mPFC-projecting vHIP neurons with a chemogenetic strategy rescues social memory deficits in ZIKV offspring mice. Our studies reveal a hyperconnectivity of vHIP to mPFC projection driving social memory deficits in mice exposed to maternal inflammation by ZIKV.
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http://dx.doi.org/10.15252/embr.202051978DOI Listing
July 2021

Measuring Metal Uptake and Loss in Individual Organisms: A Novel Double Stable Isotope Method and its Application in Explaining Body Size Effects on Cadmium Concentration in Mussels.

Environ Sci Technol 2021 Jul 30;55(14):9979-9988. Epub 2021 Jun 30.

Fujian Provincial Key Laboratory for Coastal Ecology and Environmental Studies, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian 361102, China.

Interindividual variabilities in metal bioaccumulation confound our interpretation of the biomonitoring data. Measuring metal toxicokinetics in organism "individuals" may provide insights into the processes underlying the variabilities. Therefore, we developed a double stable isotope method that can simultaneously measure uptake and elimination of metals in individual organisms and thus the distribution of the toxicokinetic parameters. Specifically, we exposed organisms to both isotopes (Cd and Cd; Cd = cadmium) during the first stage and to only one isotope (Cd) during the second stage. Metal uptake and elimination rate constants (i.e., and ) were simultaneously estimated from the content of the two isotopes measured in each organism at the end of the second stage. We applied the method to investigate the interindividual variability in Cd concentrations caused by body size in two marine mussel species. Cd concentrations are higher in larger but lower in smaller . Size-dependent Cd uptake is found to be responsible for size effects on Cd concentrations in the mussels and the interspecies differences in the relationship between Cd concentration and body size. Specifically, Cd increases with size in (0.057-0.297 L g d) but decreases with size in (0.155-0.351 L g d). In contrast, Cd is not influenced by body size (: 0.002-0.060 d; : 0.008-0.060 d). Overall, we extended the applicability of the stable isotope methods to measure metal toxicokinetics in "individual" organisms, providing a readily available tool for investigating problems related to metal bioaccumulation.
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http://dx.doi.org/10.1021/acs.est.1c01582DOI Listing
July 2021

Metabolomics analysis of poly(l-lactic acid) nanofibers' performance on PC12 cell differentiation.

Regen Biomater 2021 Aug 21;8(4):rbab031. Epub 2021 Jun 21.

State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, 2# Si Pailou, Nanjing 210096, China.

The aim of this article is to reveal the influence of aligned/random poly(l-lactic acid) (PLLA) nanofibers on PC12 cell differentiation from the perspective of metabolic level. First, three materials-PLLA aligned nanofibers (PLLA AF), PLLA random nanofibers (PLLA RF) and PLLA films (control)-were prepared by electrospinning and spin coating. Their surface morphologies were characterized. Subsequently, the cell viability, cell morphology and neurite length of PC12 cells on the surface of the three materials were evaluated, indicating more neurites in the PLLA RF groups but the longer average neurite length in the PLLA AF groups. Next, the metabolite profiles of PC12 cells cultured on the surface of the three nanofibers after 12 h, 24 h and 36 h showed that, compared with the control, 51, 48 and 31 types of differential metabolites were detected at the three time points among the AF groups, respectively; and 56, 45 and 41 types among the RF groups, respectively. Furthermore, the bioinformatics analysis of differential metabolites identified two pathways and three metabolites critical to PC12 cell differentiation influenced by the nanofibers. In addition, the verification experiment on critical metabolites and metabolic pathways were performed. The integrative analysis combining cytology, metabolomics and bioinformatics approaches revealed that though both PLLA AF and RF were capable of stimulating the synthesis of neurotransmitters, the PLLA AF were more beneficial for PC12 cell differentiation, whereas the PLLA RF were less effective.
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http://dx.doi.org/10.1093/rb/rbab031DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8218933PMC
August 2021

Parafibromin Abnormalities in Ossifying Fibroma.

J Endocr Soc 2021 Jul 8;5(7):bvab087. Epub 2021 May 8.

Center for Molecular Oncology, University of Connecticut School of Medicine, Farmington, CT 06030, USA.

Ossifying fibromas are very rare tumors that are sometimes seen as part of the hyperparathyroidism-jaw tumor syndrome (HPT-JT), which is caused by inactivating mutations of the tumor suppressor gene mutations have been identified in a subset of sporadic cases but aberrant expression of the encoded protein, parafibromin, has not been demonstrated in ossifying fibroma. We sought to determine if loss of parafibromin regularly contributes to the development of sporadic, nonsyndromic ossifying fibroma. We examined a series of 9 ossifying fibromas, including ossifying, cemento-ossifying, and juvenile active variants, for parafibromin protein expression by immunohistochemistry and for sequence abnormalities by Sanger sequencing and/or targeted AmpliSeq panel sequencing. Four ossifying fibromas showed a complete absence of nuclear parafibromin expression; loss of parafibromin expression was coupled with aberrant cytoplasmic parafibromin expression in 1 case. mutations were detected in 2 cases with aberrant parafibromin expression. These results provide novel evidence, at the level of protein expression, that loss of the parathyroid /parafibromin tumor suppressor may play a role in the pathogenesis of a subset of ossifying fibromas.
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http://dx.doi.org/10.1210/jendso/bvab087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212678PMC
July 2021

Identification and validation of inferior prognostic genes associated with immune signatures and chemotherapy outcome in acute myeloid leukemia.

Aging (Albany NY) 2021 06 18;13(12):16445-16470. Epub 2021 Jun 18.

School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing 211198, China.

Acute myeloid leukemia (AML) is a group of heterogeneous hematological malignancies. We identified key genes as and lncRNA through different bioinformatics tools. Furthermore, qPCR was performed to verify the expression level of essential genes in clinical samples. Retrospective research on 179 AML cases was used to investigate the relationship between the expression of and the characteristics of AML. The critical gene relationship with immune infiltration in AML was estimated. The clinical validation and prognostic investigation showed that , , and are highly expressed in AML ( < 0.001) and significantly associated with the overall survival in AML. Moreover, the retrospective research on 179 clinical cases showed that positive expression of is substantially related to AML classification ( < 0.001), higher count of white blood cells ( < 0.01), and poor chemotherapy outcome ( < 0.05). Furthermore, based on grouping as the high and low expression in TCGA-LAML profile, we found that genes in the highly expressed group are mainly involved in immune infiltration and inflammation-related signaling pathways. Finally, we discovered that the expression level of and lncRNA are not just closely related to the immune score and stromal score ( < 0.001) but also significantly positively correlated with various Immune signatures in AML ( < 0.001), indicating the association of these genes with immunosuppression in AML. The prediction of candidate drugs indicated that certain immunosuppressive drugs have potential therapeutic effects for AML. The critical genes could be used as potential biomarkers to evaluate the survival and prognosis of AML.
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http://dx.doi.org/10.18632/aging.203166DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8266366PMC
June 2021

Human GPR17 missense variants identified in metabolic disease patients have distinct downstream signaling profiles.

J Biol Chem 2021 Jul 16;297(1):100881. Epub 2021 Jun 16.

Herman B. Wells Center for Pediatric Research, Department of Pediatrics, Indiana University School of Medicine, Indianapolis, Indiana, USA; Center for Diabetes and Metabolic Diseases, Indiana University School of Medicine, Indianapolis, Indiana, USA; Department of Pharmacology & Toxicology, Indiana University School of Medicine, Indianapolis, Indiana, USA; Stark Neurosciences Research Institute, Indiana University School of Medicine, Indianapolis, Indiana, USA; Department of Biochemistry & Molecular Biology, Indiana University School of Medicine, Indianapolis, Indiana, USA; Department of Anatomy, Cell Biology & Physiology, Indiana University School of Medicine, Indianapolis, Indiana, USA. Electronic address:

GPR17 is a G-protein-coupled receptor (GPCR) implicated in the regulation of glucose metabolism and energy homeostasis. Such evidence is primarily drawn from mouse knockout studies and suggests GPR17 as a potential novel therapeutic target for the treatment of metabolic diseases. However, links between human GPR17 genetic variants, downstream cellular signaling, and metabolic diseases have yet to be reported. Here, we analyzed GPR17 coding sequences from control and disease cohorts consisting of individuals with adverse clinical metabolic deficits including severe insulin resistance, hypercholesterolemia, and obesity. We identified 18 nonsynonymous GPR17 variants, including eight variants that were exclusive to the disease cohort. We characterized the protein expression levels, membrane localization, and downstream signaling profiles of nine GPR17 variants (F43L, V96M, V103M, D105N, A131T, G136S, R248Q, R301H, and G354V). These nine GPR17 variants had similar protein expression and subcellular localization as wild-type GPR17; however, they showed diverse downstream signaling profiles. GPR17-G136S lost the capacity for agonist-mediated cAMP, Ca, and β-arrestin signaling. GPR17-V96M retained cAMP inhibition similar to GPR17-WT, but showed impaired Ca and β-arrestin signaling. GPR17-D105N displayed impaired cAMP and Ca signaling, but unaffected agonist-stimulated β-arrestin recruitment. The identification and functional profiling of naturally occurring human GPR17 variants from individuals with metabolic diseases revealed receptor variants with diverse signaling profiles, including differential signaling perturbations that resulted in GPCR signaling bias. Our findings provide a framework for structure-function relationship studies of GPR17 signaling and metabolic disease.
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http://dx.doi.org/10.1016/j.jbc.2021.100881DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8267566PMC
July 2021

Activated Galectin-9/Tim3 promotes Treg and suppresses Th1 effector function in chronic lymphocytic leukemia.

FASEB J 2021 07;35(7):e21556

Basic Medical College of Xinjiang Medical University, Urumqi, China.

Tim-3 is a negative immunoregulator in anti-tumor response, but its mechanism in chronic lymphocytic leukemia (CLL) is not yet clear. The aim of this study was to understand the role of Galectin-9/Tim-3 signaling pathway in the regulation of CD4 T cell subsets in CLL patients. Flow cytometry results showed that the number of Treg cells obviously increased, and there was a significant Treg/Th17 imbalance in CLL patients. In addition, Tim-3 overexpressed on the surface of Th1 and Treg cells in CLL patients. The levels of Galectin-9 and IL-10 were significantly elevated in patients of CLL, especially in stages of Binet B, and C. However, IFN-γ decreased. Moreover, Galectin-9 in CLL patients was positively correlated with the number of Tim-3 Treg cells and the level of IL-10. Interestingly, when the Tim-3/Galectin-9 pathway was blocked in vitro, the level of IL-10 in the culture supernatant of CD4 T was significantly reduced, while the levels of IFN-γ and TNF-α were increased. After co-culture with activated Th1 cells, the apoptosis of CLL cells was significantly increased, and this effect was reversed after treatment with Tim-3 Tregs. In summary, Galectin-9/Tim-3 are elevated in CLL and associated with disease progression. By the negative regulation of CD4 T cells, activated Galectin-9/Tim-3 suppresses Th1 effector function and also promotes Treg to be involved in immune escape of CLL. This pathway might become the potential target of immunotherapy in CLL patients.
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http://dx.doi.org/10.1096/fj.202100013RDOI Listing
July 2021

SARS-CoV-2 Causes Acute Kidney Injury by Directly Infecting Renal Tubules.

Front Cell Dev Biol 2021 31;9:664868. Epub 2021 May 31.

Department of Nephrology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Acute kidney injury (AKI) is one of the most prevalent complications among hospitalized coronavirus disease 2019 (COVID-19) patients. Here, we aim to investigate the causes, risk factors, and outcomes of AKI in COVID-19 patients. We found that angiotensin-converting enzyme II (ACE2) and transmembrane protease serine 2 (TMPRSS2) were mainly expressed by different cell types in the human kidney. However, in autopsy kidney samples, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleoprotein was detected in ACE2 or TMPRSS2 renal tubular cells, whereas the RNAscope Assay targeting the SARS-CoV-2 Spike gene was positive mainly in the distal tubular cells and seldom in the proximal tubular cells. In addition, the TMPRSS2 and kidney injury marker protein levels were significantly higher in the SARS-CoV-2-infected renal distal tubular cells, indicating that SARS-CoV-2-mediated AKI mainly occurred in the renal distal tubular cells. Subsequently, a cohort analysis of 722 patients with COVID-19 demonstrated that AKI was significantly related to more serious disease stages and poor prognosis of COVID-19 patients. The progressive increase of blood urea nitrogen (BUN) level during the course of COVID-19 suggests that the patient's condition is aggravated. These results will greatly increase the current understanding of SARS-CoV-2 infection.
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http://dx.doi.org/10.3389/fcell.2021.664868DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201778PMC
May 2021

A cohort autopsy study defines COVID-19 systemic pathogenesis.

Cell Res 2021 Jun 16. Epub 2021 Jun 16.

Department of Critical Care Medicine, PLA Key Laboratory of Emergency and Critical Care Research, Jinling Hospital, Nanjing University, Nanjing, Jiangsu, China.

Severe COVID-19 disease caused by SARS-CoV-2 is frequently accompanied by dysfunction of the lungs and extrapulmonary organs. However, the organotropism of SARS-CoV-2 and the port of virus entry for systemic dissemination remain largely unknown. We profiled 26 COVID-19 autopsy cases from four cohorts in Wuhan, China, and determined the systemic distribution of SARS-CoV-2. SARS-CoV-2 was detected in the lungs and multiple extrapulmonary organs of critically ill COVID-19 patients up to 67 days after symptom onset. Based on organotropism and pathological features of the patients, COVID-19 was divided into viral intrapulmonary and systemic subtypes. In patients with systemic viral distribution, SARS-CoV-2 was detected in monocytes, macrophages, and vascular endothelia at blood-air barrier, blood-testis barrier, and filtration barrier. Critically ill patients with long disease duration showed decreased pulmonary cell proliferation, reduced viral RNA, and marked fibrosis in the lungs. Permanent SARS-CoV-2 presence and tissue injuries in the lungs and extrapulmonary organs suggest direct viral invasion as a mechanism of pathogenicity in critically ill patients. SARS-CoV-2 may hijack monocytes, macrophages, and vascular endothelia at physiological barriers as the ports of entry for systemic dissemination. Our study thus delineates systemic pathological features of SARS-CoV-2 infection, which sheds light on the development of novel COVID-19 treatment.
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http://dx.doi.org/10.1038/s41422-021-00523-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8208380PMC
June 2021

Clinical effects of direct anterior internal fixation with Herbert screws on hip joint function and quality of life in patients with Pipkin II femoral head fractures.

Arch Orthop Trauma Surg 2021 Jun 14. Epub 2021 Jun 14.

Department of Traumatic Orthopedics, Renmin Hospital, Hubei University of Medicine, No.39 Chaoyang Road, Maojian, Shiyan, 442000, Hubei, China.

Objective: The study aimed to explore the therapeutic effects of direct anterior internal fixation with Herbert screws on hip joint function and quality of life in patients with Pipkin type II femoral head fractures (FHF).

Methods: From Dec 2017 to Jul 2020, 68 patients with Pipkin type II FHF were received in our hospital and divided into two groups. The direct anterior internal fixation (DAIF) group including 34 cases were treated by direct anterior internal fixation with Herbert screws. The control group of 34 patients received modified internal fixation with Herbert screws via posterior superior iliac spine and ectogluteus. The duration time and blood loss in operation as well as the postoperative drainage volume, hospital stays and complications were observed. The comparison of pain degree, hip functions, and life quality between two groups was performed.

Results: All the patients were followed up, and the operative time, intraoperative blood loss, postoperative drainage and hospital stay of the DAIF group were all significantly lower than those in the control group, with (p < 0.05, respectively). The pain degree of the DAIF group was significantly lower than that of the control group 7, 15 and 30 days after the operation (p < 0.05, respectively). At 3, 6 and 9 months after the operation, the hip function recovery of the DAIF group was significantly better than control group (p < 0.05). There were no significant differences between the two groups in preoperative physiological function, physiological function, emotional role, physical pain, general health, vitality, social function and mental health (p > 0.05). Six months after the operation, the physiological function, physiological function, emotional role, physical pain, general health, vitality, social function and mental health of the DAIF group were significantly higher than those of the control group (p < 0.05). No postoperative complications occurred in both groups.

Conclusion: The treatment of directly anterior internal fixation with Herbert screws is effective for Pipkin type II FHF, like improving the function of hip joint and quality of life in patients. The method is reliable and worth clinical use.
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http://dx.doi.org/10.1007/s00402-021-03973-2DOI Listing
June 2021

Erratum: Assessment of radiotherapy effect for nasopharyngeal cancer using plasma surface-enhanced Raman spectroscopy technology: errata.

Biomed Opt Express 2021 May 2;12(5):2557-2558. Epub 2021 Apr 2.

Fujian Normal University, Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fuzhou, 350007, China.

[This corrects the article on p. 3413 in vol. 9, PMID: 29984106.].
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http://dx.doi.org/10.1364/BOE.426301DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176798PMC
May 2021

Ganoderma lucidum polysaccharide modulates gut microbiota and immune cell function to inhibit inflammation and tumorigenesis in colon.

Carbohydr Polym 2021 Sep 20;267:118231. Epub 2021 May 20.

College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, PR China. Electronic address:

This study investigated the effects of water-soluble polysaccharide extracted from the sporoderm-removed spores of Ganoderma lucidum (GLP) against AOM/DSS-induced inflammation, tumorigenesis, and gut microbiota modification, which has never been reported before. Our data revealed that GLP (200 and 300 mg/kg) decreased AOM/DSS-induced colitis and tumorigenesis, manifested by significantly reduced disease activity index score, and total number and size of tumors. Furthermore, GLP ameliorated AOM/DSS-induced microbiota dysbiosis, increased short-chain fatty acid production, and alleviated endotoxemia by inhibiting TLR4/MyD88/NF-κB signaling. Besides, GLP profoundly improved gut barrier function as evidenced by increased numbers of goblet cells, MUC2 secretion, and tight junction protein expressions. GLP treatment inhibited macrophage infiltration and downregulated IL-1β, iNOS, and COX-2 expressions. Additionally, GLP inhibited lipopolysaccharides (LPS)-induced inflammation markers and MAPK (JNK and ERK) activation in macrophage RAW264.7, intestinal HT-29, and NCM460 cells. In conclusion, these results indicate that GLP is a promising prebiotic for the treatment of colorectal cancer.
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http://dx.doi.org/10.1016/j.carbpol.2021.118231DOI Listing
September 2021

Light-Fueled Beating Coffee-Ring Deposition for Droplet Evaporative Crystallization.

Anal Chem 2021 06 10;93(25):8817-8825. Epub 2021 Jun 10.

Key Laboratory of Low-grade Energy Utilization Technologies and Systems, Chongqing University, Ministry of Education, Chongqing 400030, China.

Condensed deposition favors biochemical analysis, bioassays, and clinical diagnosis, but the existing strategies may suffer from low resolution, inaccurate control, cross-contamination, or miscellaneous apparatus. Herein, we propose a noncontact light strategy to enable the condensed deposition for droplet evaporative crystallization, in which the photothermal effect of a focused infrared laser is employed to induce intense evaporation. Due to the localized heating effect, not only can the droplet evaporative crystallization on the hydrophobic substrate be promoted, but also the resultant intensified Marangoni flow enables the movement of the early-formed crystals, preventing the pinning of the triple-phase contact line. Synergy of the Marangoni flow and nonuniform evaporation makes the solutes tend to accumulate near the focused light beam region, which facilitates the condensed deposition. More importantly, this light strategy not only enables condensed deposition on the hydrophobic surface with low hysteresis, but also works successfully on the hydrophilic substrate with high hysteresis via adjusting input laser power. It is demonstrated that the light strategy proposed in the present study has great potential for relevant applications.
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http://dx.doi.org/10.1021/acs.analchem.1c00605DOI Listing
June 2021

Incidence and Risk Factors of In-Hospital Prosthesis-Related Complications Following Total Knee Arthroplasty: A Retrospective Nationwide Inpatient Sample Database Study.

Orthop Surg 2021 Jul 9;13(5):1579-1586. Epub 2021 Jun 9.

Division of Orthopaedic Surgery, Department of Orthopaedics, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Objective: To examine the incidence and risk factors of in-hospital prosthesis-related complications (PRCs) following total knee arthroplasty (TKA) using a large-scale national database.

Methods: A retrospective database analysis was performed based on Nationwide Inpatient Sample (NIS) from 2005-2014. Patients who underwent TKA were included. The recruited cases were divided into two groups according to the occurrence of PRCs. Patient demographics (age, sex, and race), hospital characteristics (type of admission and payer, and bedsize, teaching status, location, and region of hospital), length of stay (LOS), total charges during hospitalization, in-hospital mortality, comorbidities, and perioperative complications were analyzed.

Results: A total of 1,227,244 TKAs were captured from the NIS database. There were 8484 cases of in-hospital PRCs after TKA and the overall incidence was 0.69%, with a slight downward trend annually. Periprosthetic joint infection (PJI) was the main category among PRCs (0.20%), followed by mechanical loosening (0.04%), dislocation (0.02%), and periprosthetic fracture (PPF) (0.01%). Patients suffered from in-hospital PRCs were 3 years younger (64 years vs 67 years) and 6.51% more likely to be male (43.60% vs 37.09%) compared to the nonaffected population (P < 0.0001). Additionally, patients experiencing in-hospital PRCs after TKA were 2.11% less likely through elective admission (92.07% vs 94.18%) while 2.34% more likely in teaching hospital (45.53% vs 43.19%) than those without these complications (P < 0.0001). Furthermore, the occurrence of in-hospital PRCs was associated with longer LOS (4 days vs 3 days; P < 0.0001), more total charges ($53,418 vs $41,204, P < 0.0001), and higher in-hospital mortality (0.30% vs 0.07%; P < 0.0001). Multivariate logistic regression was performed to identify independent risk factors of in-hospital PRCs after TKA which included younger age, male, non-elective admission, teaching hospital, deficiency and chronic blood loss anemia, coagulopathy, congestive heart failure, depression, diabetes with chronic complications, fluid and electrolyte disorders, pulmonary circulation disorders, metastatic cancer, and weight loss. Besides, in-hospital PRCs after TKA were associated with secondary osteoarthritis, inflammatory arthritis, prior knee arthroscopy, acute renal failure, acute myocardial infarction, deep vein thrombosis, sepsis, transfusion, and wound dehiscence.

Conclusion: It is beneficial to study the risk factors of in-hospital PRCs after TKA to ensure the appropriate management and optimize consequences although a relatively low incidence was identified.
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http://dx.doi.org/10.1111/os.13008DOI Listing
July 2021

TMK1-based auxin signaling regulates abscisic acid responses via phosphorylating ABI1/2 in .

Proc Natl Acad Sci U S A 2021 Jun;118(24)

FAFU-UCR Joint Center, Horticulture and Metabolic Biology Center, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, People's Republic of China;

Differential concentrations of phytohormone trigger distinct outputs, which provides a mechanism for the plasticity of plant development and an adaptation strategy among plants to changing environments. However, the underlying mechanisms of the differential responses remain unclear. Here we report that a high concentration of auxin, distinct from the effect of low auxin concentration, enhances abscisic acid (ABA) responses in , which partially relies on TRANS-MEMBERANE KINASE 1 (TMK1), a key regulator in auxin signaling. We show that high auxin and TMK1 play essential and positive roles in ABA signaling through regulating ABA INSENSITIVE 1 and 2 (ABI1/2), two negative regulators of the ABA pathway. TMK1 inhibits the phosphatase activity of ABI2 by direct phosphorylation of threonine 321 (T321), a conserved phosphorylation site in ABI2 proteins, whose phosphorylation status is important for both auxin and ABA responses. This TMK1-dependent auxin signaling in the regulation of ABA responses provides a possible mechanism underlying the high auxin responses in plants and an alternative mechanism involved in the coordination between auxin and ABA signaling.
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http://dx.doi.org/10.1073/pnas.2102544118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8214701PMC
June 2021

Inherently Area-Selective Atomic Layer Deposition of Manganese Oxide through Electronegativity-Induced Adsorption.

Molecules 2021 May 20;26(10). Epub 2021 May 20.

State Key Laboratory of Digital Manufacturing Equipment and Technology, School of Mechanical Science and Engineering, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430063, China.

Manganese oxide (MnO) shows great potential in the areas of nano-electronics, magnetic devices and so on. Since the characteristics of precise thickness control at the atomic level and self-align lateral patterning, area-selective deposition (ASD) of the MnO films can be used in some key steps of nanomanufacturing. In this work, MnO films are deposited on Pt, Cu and SiO substrates using Mn(EtCp) and HO over a temperature range of 80-215 °C. Inherently area-selective atomic layer deposition (ALD) of MnO is successfully achieved on metal/SiO patterns. The selectivity improves with increasing deposition temperature within the ALD window. Moreover, it is demonstrated that with the decrease of electronegativity differences between M (M = Si, Cu and Pt) and O, the chemisorption energy barrier decreases, which affects the initial nucleation rate. The inherent ASD aroused by the electronegativity differences shows a possible method for further development and prediction of ASD processes.
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http://dx.doi.org/10.3390/molecules26103056DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8161048PMC
May 2021

COVID-19-another influential event impacts on laboratory medicine management.

J Clin Lab Anal 2021 Jun 25;35(6):e23804. Epub 2021 May 25.

Shanghai center for clinical laboratory, Shanghai, China.

Background: Before public health emergencies became a major challenge worldwide, the scope of laboratory management was only related to developing, maintaining, improving, and sustaining the quality of accurate laboratory results for improved clinical outcomes. Indeed, quality management is an especially important aspect and has achieved great milestones during the development of clinical laboratories.

Current Status: However, since the coronavirus disease 2019 (COVID-19) pandemic continues to be a threat worldwide, previous management mode inside the separate laboratory could not cater to the demand of the COVID-19 public health emergency. Among emerging new issues, the prominent challenges during the period of COVID-19 pandemic are rapid-launched laboratory-developed tests (LDTs) for urgent clinical application, rapid expansion of testing capabilities, laboratory medicine resources, and personnel shortages. These related issues are now impacting on clinical laboratory and need to be effectively addressed.

Conclusion: Different from traditional views of laboratory medicine management that focus on separate laboratories, present clinical laboratory management must be multidimensional mode which should consider consolidation of the efficient network of regional clinical laboratories and reasonable planning of laboratories resources from the view of overall strategy. Based on relevant research and our experience, in this review, we retrospect the history trajectory of laboratory medicine management, and also, we provide existing and other feasible recommended management strategies for laboratory medicine in future.
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http://dx.doi.org/10.1002/jcla.23804DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8183907PMC
June 2021
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